CN106861779A - A kind of segmented micro-fluidic chip temperature regulating device - Google Patents
A kind of segmented micro-fluidic chip temperature regulating device Download PDFInfo
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- CN106861779A CN106861779A CN201710090572.7A CN201710090572A CN106861779A CN 106861779 A CN106861779 A CN 106861779A CN 201710090572 A CN201710090572 A CN 201710090572A CN 106861779 A CN106861779 A CN 106861779A
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- temperature
- hot plate
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- temperature control
- fluidic chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1894—Cooling means; Cryo cooling
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Control Of Temperature (AREA)
Abstract
The invention discloses the temperature control platform that a kind of segmented controls micro-fluidic chip temperature, including temperature control platform, intelligent temperature regulator and constant temperature circulator, the temperature control platform ecto-entad is made up of the upward semiconductor chilling plate of shell, cold drawing and hot plate, huyashi-chuuka (cold chinese-style noodles) and heater, radiator, metal catheter, there is temperature meter in cold-hot plate.The present apparatus has used high-performance micro semiconductor chilling plate and ceramic heating flake, greatly reduces device volume, the characteristics of area is small in itself for micro-fluidic chip, improves the practicality of temperature control platform;Hot plate and the temperature data of cold drawing that the present apparatus is collected using commercial temperature controller Tempco TEC 220 according to platinum electrode, by the operation and stopping of precise control semiconductor chilling plate and ceramic heating flake, realize High Precision Automatic temperature control;Using the type constant temperature circulators of HX 1050, the temperature range of circulating liquid is set to be maintained at 60 DEG C 50 DEG C, meet the cooling requirements of the present apparatus, reduce experiment energy consumption, reduce use cost, it is adaptable to which biological PCR, drop and capsule solidification thawings, crystal-phase transformation, materials synthesis during temperature control realizes micro-fluidic chip in the micro-fluidic chip etc. are tested.
Description
Technical field
The invention belongs to thermoelectric applied technical field, more particularly to a kind of segmented micro-fluidic chip temperature regulating device.
Background technology
With continuing to develop and maturation, increasing various biological and chemical experimental examples such as numeral for microflow control technique
PCR, cell culture, materials synthesis, drug screening etc. will all be realized in a slight micro-fluidic chip.Generally these experiments
It is required for smoothly reacting at a certain temperature, this temperature range is general at -40-100 DEG C.
At present, the above-mentioned experiment in micro-fluidic chip is all mostly by using high-low temperature chamber or temperature cycling test
Case carries out temperature control to be tested, and not only temperature-controlled precision is relatively low for these equipment, and operating temperature range is also smaller, and volume and work(
Consumption is all very big.
The content of the invention
The present invention is solution problems of the prior art, there is provided a kind of temperature control equipment, the present apparatus has control
Warm high precision, operating temperature range are big, good heat dissipation effect, small energy consumption the characteristics of.
The purpose of the present invention is achieved through the following technical solutions:
A kind of segmented micro-fluidic chip temperature regulating device, it is characterised in that:Including temperature control platform, intelligent temperature regulator,
And constant-temperature circulating device, the temperature control platform ecto-entad by the upward semiconductor chilling plate of shell, cold drawing and hot plate, huyashi-chuuka (cold chinese-style noodles),
Heater, radiator, metal catheter are constituted, and have temperature meter in cold-hot plate.Intelligent temperature regulator and heater and the temperature difference
Electric refrigerating assembly is connected;The delivery port of water jacket, going out on constant temperature circulator in import connection temperature control platform on constant temperature circulator
The water inlet of water jacket in mouth of a river connection temperature control platform.
Further, the temperature control platform is fixed on using among 3D printing shell out, the cold drawing of temperature control platform and
Hot plate is the copper structure of stepped ramp type, and the boss position of cold drawing and hot plate is embedded in the square hole of shell upper, boss upper surface with
Case surface is in one plane;Hot and cold plate is be bonded using heat conductive silica gel with thermoelectric cooling module, and cold drawing and hot plate side have
One hole, the axis in hole is parallel with cold drawing upper surface, and the temperature meter is the PT100 platinum being placed in cold drawing and hot plate lateral aperture
Electrode;Thermoelectric cooling module is by high performance micro semiconductor refrigerator (M of a Ferrotec 9503/035/025) structure
Into the huyashi-chuuka (cold chinese-style noodles) of thermoelectric cooling module, hot face are coated with heat-conducting silicone grease;Described heater assembly is by ceramic heater structure
Into heater is linked together using heat conductive silica gel with hot plate;Radiator is copper stepped part, and there is U-shaped groove inside
For installing coolant duct, shell side face corresponding position has water jacket water inlet and water jacket delivery port, and the constant temperature circulator is
The HX-1050 type constant temperature circulators being placed in outside casing.
Further, the cooling piece and heating plate are micro semiconductor cooling piece and miniature ceramic heating flake.
Further, the shell forms the size and number that can as needed change cold drawing and hot plate for 3D printing.
Further, the temperature control platform is made up of multiple independent cold drawings and hot plate, each cold drawing and hot plate temperature
Independent control is independent of each other.
Preferably, the conduit is red copper conduit.
The present invention has the advantages and positive effects that:
1st, using the high performance micro semiconductor refrigerator (M of Ferrotec 9503/035/025) of import, it is ensured that this
Device has enough refrigeration depth and refrigeration speed;Refrigerated medium hot arc is divided into seven sections, and refrigeration section is all by an independence
The semiconductor chilling plate of control carrys out regulating and controlling temperature, heats area and comes regulating and controlling temperature, the temperature of different sections by an independent heater
Degree can be independent control;High Precision Automatic temperature control is realized by Tempco TEC-220 program-type intelligent temperature regulators, temperature
Control scope and precision reach -40 DEG C -100 DEG C (± 0.2 DEG C), and the minisize refrigeration piece for using and hot meet micro-fluidic chip body
The characteristics of product is small, can substantially reduce the number experiment energy consumption, reduce use cost, whole temperature measuring area uniform distribution of temperature field, steady
It is fixed.
2nd, using the external HX-1050 type constant temperature circulators with automatic pid control function, by circulating liquid by the temperature difference
The used heat that the hot face of electric refrigerating assembly produces is taken away, by automatic PID control, make circulating liquid temperature range be maintained at -10 DEG C -
50 DEG C, meet the cooling requirements of the present apparatus.
3rd, using Tempco TEC-220 program-type intelligent temperature regulators, realize that man-machine interaction is used, and and computer
Real-time communication, it is ensured that temperature control is accurately and reliably.
4th, the present apparatus suitable for the temperature control the micro-fluidic chip realize micro-fluidic chip in biological PCR, drop and capsule
The experiments such as solidification thawing, crystal-phase transformation, materials synthesis.
Brief description of the drawings
Fig. 1 is temperature regulating device appearance schematic diagram;
Fig. 2 is temperature regulating device schematic front view in Fig. 1;
Fig. 3 is A-A schematic cross-sectional views in Fig. 2;
Specific embodiment
The invention will be further described below in conjunction with the accompanying drawings and by the way that example is embodied, and following embodiment is to retouch
The property stated, be not limited, it is impossible to limit protection scope of the present invention with this.
Refer to Fig. 1 to Fig. 3:From left to right successively by intelligent temperature regulator 1, temperature control platform, and constant-temperature circulating device
12 constitute segmented micro-fluidic chip temperature regulating device, have the com used with external connection of computer to connect in the intelligent temperature regulator
Mouth 4, the module 2 being connected with temperature meter 22, the module 3 being connected with heating plate and cooling piece 21, the temperature control platform is by outer
Inwardly by shell 8, cold drawing 10 and hot plate 7,9,11, the upward semiconductor chilling plate 21 of huyashi-chuuka (cold chinese-style noodles) and heating plate, radiator 16, metal
Conduit 17,18 is constituted, and has temperature meter 22 in cold-hot plate.Intelligent temperature regulator and heater and thermoelectric cooling module it
Between using copper conductor 5 be connected, be connected using copper conductor 6 between intelligent temperature regulator and temperature meter;Constant temperature circulator 12
On import 14 be connected with the delivery port 18 of copper pipe in temperature control platform using rubber tube 13, the delivery port 15 on constant temperature circulator connects
Connect the water inlet 17 of copper pipe in temperature control platform.
Temperature control platform is fixed among use 3D printing shell out, and the cold drawing and hot plate of temperature control platform are stepped ramp type copper
The boss position of matter structure, cold drawing and hot plate is embedded in the square hole of shell upper, and boss upper surface is with case surface at one
In plane;Cold drawing is be bonded using heat conductive silica gel with thermoelectric cooling module, and there is a hole cold drawing and hot plate side, the axis in hole with it is cold
Plate upper surface is parallel, and the temperature meter is the PT100 platinum electrodes being placed in cold drawing and hot plate lateral aperture;Thermoelectric (al) cooling group
Part is made up of a high performance micro semiconductor refrigerator (M of Ferrotec 9503/035/025), difference electricity refrigerating assembly
Huyashi-chuuka (cold chinese-style noodles), hot face are coated with heat-conducting silicone grease;Radiator is copper stepped part, and there is U-shaped groove inside for installing cooling
Liquid pipe road, shell side face corresponding position has water jacket water inlet and water jacket delivery port, and the constant temperature circulator is to be placed in outside casing
HX-1050 type constant temperature circulators.
Tempco TEC-220 program-types intelligent temperature regulators realize Tempco TEC-220 programs by RS232COM
Type intelligent temperature regulator and the connection of host computer, complete real-time communication and data-transformation facility;Tempco TEC-220 programs
Type intelligent temperature regulator is joined directly together with cooling piece and heater, works as electric power starting, Tempco TEC-220 program-type intelligence
Thermoregulator ensure that the M thermoelectric cooling modules of Ferrotec 9503/035/025 and heater normal DC are powered,
Ensure the refrigeration demand of refrigerator and the demand for heat of heater;Tempco TEC-220 program-types intelligent temperature regulators with
PT100 RTD terminals are connected, by PT100 RTDs for Tempco TEC-220 program-type intelligent temperature regulators are provided
Resistance |input paramete, realizes temperature real-time monitoring.
Temperature control
When Tempco TEC-220 program-type intelligent temperature regulators switch on power, Tempco TEC-220 program-type intelligence
Thermoregulator receives the temperature feedback information from PT100 RTDs, by programme-control Ferrotec 9503/035/025
The startup of M thermoelectric cooling modules and ceramic heater and the state that is stopped, realize the temperature control requirement of temperature controller, full
- 40 DEG C -100 DEG C of foot, the requirement of temperature-controlled precision ± 0.2 DEG C.Tempco TEC-220 program-types intelligent temperature regulator ensures dress
The requirement of heating-cooling speed is put, when setting speed more than system maximum parameter, system automatic time delay is according to maximum rate parameter liter
Cooling.Temperature control method can also be set using segmented program as needed, realize temperature monitoring, implementation procedure control and real-time people
Machine is operated, information mutual communication, it is ensured that temperature control is accurately and reliably.Tempco TEC-220 program-type intelligent temperature regulators possess PID certainly
Setting function, is capable of achieving the heating-cooling control of any slope, with the programmable operations order such as cycling jump, operation, pause,
Program is changed in permission at any time in program operation process.HX-1050 types constant temperature circulator radiates as external liquid SAPMAC method and fills
Put, with circulating liquid as carrier, it is ensured that fluid homeostasis at the uniform velocity flow, there is provided the power of liquid-circulating heat radiation, by Ferrotec
The used heat that the hot face of 9503/035/025 M thermoelectric cooling modules produces is taken away, and circulating liquid temperature-control range is maintained at -10 DEG C -50
DEG C, fully meet high-low temperature tester radiating requirements.
The operation principle of the present apparatus:Sufficiently large refrigerating capacity is locally being produced using thermoelectric cooling module, is being realized enough
Big refrigeration depth and sufficiently fast refrigeration speed, is heated using ceramic heating flake to hot plate, realizes the temperature control of hot plate
System, using Tempco TEC-220 program-types intelligent temperature regulators by collecting the feedback parameter of temperature meter, automatic control
On experiment porch processed in each hot plate and cold drawing heating plate and cooling assembly operation and stopping, realizing each in operation temperature area
The High Precision Automatic temperature control of temperature.
Specific embodiment of the invention is described above.It is to be appreciated that the invention is not limited in above-mentioned
Particular implementation, those skilled in the art can within the scope of the claims make various deformations or amendments, this not shadow
Sound substance of the invention.
Claims (6)
1. a kind of segmented micro-fluidic chip temperature regulating device, it is characterised in that:Including temperature control platform, intelligent temperature regulator and
Constant-temperature circulating device, the temperature control platform ecto-entad by the upward semiconductor chilling plate of shell, cold drawing and hot plate, huyashi-chuuka (cold chinese-style noodles), plus
Hot device, radiator, metal catheter are constituted, and have temperature meter, intelligent temperature regulator and heater and thermoelectric in cold-hot plate
Refrigerating assembly is connected;The delivery port of water jacket, the water outlet on constant temperature circulator in import connection temperature control platform on constant temperature circulator
The water inlet of water jacket in mouth connection temperature control platform.
2. segmented micro-fluidic chip temperature regulating device according to claim 1, it is characterised in that:The temperature control platform is fixed
Among using 3D printing shell out, the cold drawing and hot plate of temperature control platform are the copper structure of stepped ramp type, cold drawing and hot plate
Boss position is embedded in the square hole of shell upper, and boss upper surface is with case surface in one plane;Cold drawing and thermoelectric
Refrigerating assembly is bonded using heat conductive silica gel, and hot plate is be bonded using heat conductive silica gel with heater assembly, and cold drawing and hot plate side have one
Hole, the axis in hole is parallel with cold drawing upper surface, and the temperature meter is the PT100 platinum electricity being placed in cold drawing and hot plate lateral aperture
Pole;Thermoelectric cooling module is made up of a high performance micro semiconductor refrigerator (Ferrotec 9503/035/025M),
The huyashi-chuuka (cold chinese-style noodles) of difference electricity refrigerating assembly, hot face are coated with heat-conducting silicone grease;Described heater assembly is made up of ceramic heater;Radiating
Device is copper stepped part, and there is U-shaped groove inside for installing coolant duct, and shell side face corresponding position has water jacket to enter
The mouth of a river and water jacket delivery port, the constant temperature circulator are the HX-1050 type constant temperature circulators being placed in outside casing.
3. segmented micro-fluidic chip temperature regulating device according to claim 1 and 2, it is characterised in that:The cooling piece and
Heating plate is micro semiconductor cooling piece and miniature ceramic heating flake.
4. segmented micro-fluidic chip temperature regulating device according to claim 1 and 2, it is characterised in that:The shell is 3D
Printing forms the size and number that can as needed change cold drawing and hot plate.
5. segmented micro-fluidic chip temperature regulating device according to claim 1 and 2, it is characterised in that:The temperature control platform
It is made up of multiple independent cold drawings and hot plate, each cold drawing and hot plate temperature independent control are independent of each other.
6. segmented micro-fluidic chip temperature regulating device according to claim 1 and 2, it is characterised in that:The conduit is purple
Copper conductor tube.
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Cited By (5)
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CN110270387A (en) * | 2019-06-11 | 2019-09-24 | 南京理工大学 | A kind of accurate radiator and its control method based on electrowetting on dielectric |
WO2019184913A1 (en) * | 2018-03-28 | 2019-10-03 | 京东方科技集团股份有限公司 | Microfluidic control chip and preparation method therefor, and drive method |
CN110554651A (en) * | 2019-09-19 | 2019-12-10 | 哈尔滨工业大学 | Private Internet of things system for measuring and controlling temperature of microfluidic chip |
CN110888471A (en) * | 2018-09-07 | 2020-03-17 | 中兴通讯股份有限公司 | Terminal heat treatment method, device, equipment and storage medium |
WO2023005796A1 (en) * | 2021-07-28 | 2023-02-02 | 京东方科技集团股份有限公司 | Digital microfluidic apparatus and driving method therefor |
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