CN213012879U - PCR augmentor - Google Patents
PCR augmentor Download PDFInfo
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- CN213012879U CN213012879U CN202021271346.2U CN202021271346U CN213012879U CN 213012879 U CN213012879 U CN 213012879U CN 202021271346 U CN202021271346 U CN 202021271346U CN 213012879 U CN213012879 U CN 213012879U
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- peltier
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Abstract
The utility model discloses a PCR augmentor relates to biomolecule instrument field, including casing, sample loading module, peltier and circulating fan, sample loading module sets up in the casing and sample loading module is used for the holding sample, and peltier sets up in the below of sample loading module, can generate heat or refrigerate after peltier circular telegram, and circulating fan sets up the one side at peltier to make circulating fan can blow the air that peltier refrigerates or heats to sample loading module. The sample loading module is used for placing a PCR sample needing amplification, and the PCR sample can be refrigerated or heated after being electrified by the Peltier, so that air in the shell can be heated or refrigerated, the circulating fan can circulate hot gas or cold gas in the shell, the sample placed on the sample recording module can be rapidly heated or cooled, and the amplification rate of the PCR sample is improved.
Description
Technical Field
The utility model relates to the technical field of biological instruments, in particular to a PCR amplification instrument.
Background
Polymerase Chain Reaction (PCR) is a molecular biology technique for amplifying and amplifying specific DNA fragments, and can be regarded as special DNA replication in vitro, and the greatest feature of PCR is that a trace amount of DNA can be greatly increased. PCR consists of several steps of high temperature denaturation, low temperature annealing, proper temperature extension and other reactions, and is performed circularly to amplify target DNA fast, and has the features of high specificity, high sensitivity, simple operation, saving in time, etc. It can be used not only for basic research of gene separation, cloning and nucleic acid sequence analysis, but also for treating infectious diseases, tumors and genetic diseases, so to speak, PCR is needed in places where DNA and RNA are researched. PCR is therefore immediately widely used and rapidly developed in many fields such as molecular biology, microbiology, medicine and genetics. In the PCR amplification process, the module with the sample needs to be rapidly heated and cooled, so that the purposes of performing high-temperature denaturation, low-temperature annealing and proper-temperature extension on the sample are achieved. The conventional PCR amplification instrument generally adopts a PID temperature control mode to directly control the temperature, the temperature of a heat conduction module is kept unchanged after reaching a target temperature, the temperature rise of a module provided with a sample has hysteresis, the temperature rise rate is slower and slower along with the temperature rise, the maximum temperature rise and fall rate is 2-3 ℃ per second, the temperature rise and fall rate can prolong the time required for completing the PCR amplification, and the experiment cost is high due to the long service cycle.
SUMMERY OF THE UTILITY MODEL
In view of this, the utility model aims at overcoming the not enough of prior art, provide a PCR amplificator to solve the inefficiency of current amplificator intensification and cooling and lead to the problem that PCR amplificator is inefficient.
The utility model provides a technical scheme that its technical problem adopted is:
the PCR amplification instrument comprises a shell, a sample loading module, a Peltier and a circulating fan, wherein the sample loading module is arranged in the shell and used for accommodating a sample, the Peltier is arranged below the sample loading module, the Peltier can be heated or cooled after being electrified, and the circulating fan is arranged on one side of the Peltier so that the circulating fan can blow air cooled or heated by the Peltier to the sample loading module.
On the basis of the technical scheme, the utility model discloses can also do following improvement.
Further, the temperature control circuit board is arranged below the Peltier, the Peltier is mounted on the temperature control circuit board, and the temperature control circuit board provides electric energy for the Peltier.
Further, still include temperature detect sensor, temperature detect sensor sets up on the control by temperature change circuit board, temperature detect sensor is used for the temperature in the monitoring casing.
And the temperature protection switch is arranged on the temperature control circuit board and used for implementing power-off protection when the temperature in the shell exceeds a process set value.
Further, the circulating fan is provided with a plurality of circulating fans, and the plurality of circulating fans are arranged around the Peltier.
Further, the sample loading module comprises a sample groove and a gene chip, and the gene chip is arranged in the sample groove.
Further, a guide edge is arranged in the sample groove and used for limiting the gene chip to prevent the gene chip from moving.
Further, a heat radiation fan located below the Peltier is arranged in the shell.
Furthermore, heat dissipation holes used for discharging heat in the shell are formed in two side walls of the shell, and a perspective window used for observing the sample loading module is arranged at the top of the shell.
Further, the highest temperature rise rate in the shell is 10 ℃/s, and the highest temperature drop rate in the shell is 8 ℃/s.
Compared with the prior art, the utility model discloses the beneficial effect who has is:
the utility model provides a can refrigerate or heat after the circular telegram of peltier to air in the casing heats or refrigerates, and circulating fan can be with hot gas or cold gas at the casing internal loop, makes the sample of placing on sample record module can heat up fast or cool down, thereby improves the amplification rate of PCR sample.
The utility model discloses other beneficial effects that still have are:
the utility model provides a temperature control circuit board provides the power for the paler subsides, and temperature control passes through temperature control circuit board system and realizes according to the power supply mode of settlement technology, real-time acquisition temperature detection sensor signal and through controlling inside circulating fan in the casing. The utility model discloses can carry out temperature compensation to the gene chip that is close to sample loading module lateral wall, reduce edge effect for each gene chip is heated evenly, thereby has effectively improved PCR amplification's heating and cooling rate, shortens PCR amplification required time. In addition, the perspective window is arranged on the top of the shell, so that the abnormal condition of the internal gene chip in the amplification process can be observed in real time in the amplification process.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic view of the present invention;
fig. 2 is a schematic view of a peltier device of the present invention;
fig. 3 is a schematic view of the heat dissipation holes of the present invention.
Wherein the figures include the following reference numerals: 100-shell, 110-cooling fan, 120-cooling hole, 130-perspective window, 200-sample loading module, 210-sample groove, 220-gene chip, 300-Peltier, 400-circulating fan, 500-temperature control circuit board, 510-temperature detection sensor and 520-temperature protection switch.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be described in detail below. It is to be understood that the embodiments described are merely exemplary of the invention and are not intended to be exhaustive. Based on the embodiments of the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
Example 1:
a PCR amplification instrument, as shown in FIGS. 1-3, comprises a housing 100, a sample loading module 200, a Peltier 300 and a circulating fan 400, wherein the sample loading module 200 is arranged in the housing 100, the sample loading module 200 is used for accommodating a sample, the Peltier 300 is arranged below the sample loading module 200, the Peltier 300 can generate heat or cool after being electrified, and the circulating fan 400 is arranged at one side of the Peltier 300, so that the circulating fan 400 can blow air cooled or heated by the Peltier 300 to the sample loading module 200.
The sample loading module 200 is used for placing a PCR sample to be amplified, and the Peltier 300 can be cooled or heated after being electrified, so that air in the shell 100 can be heated or cooled, and the circulating fan 400 can circulate hot gas or cold gas in the shell 100, so that the sample placed on the sample recording module can be rapidly heated or cooled, and the amplification rate of the PCR sample is improved.
In the present embodiment, a temperature control circuit board 500 is further provided, the temperature control circuit board 500 is disposed below the peltier 300, the peltier 300 is mounted on the temperature control circuit board 500 and the temperature control circuit board 500 provides power to the peltier 300, the temperature control circuit board 500 functions as a power supply, and at the same time, it can adjust the heating or cooling temperature of the peltier 300, so that the temperature inside the casing 100 conforms to the actually required temperature.
In this embodiment, a temperature detection sensor 510 is further provided, the temperature detection sensor 510 is disposed on the temperature control circuit board 500, the temperature detection sensor 510 is used for monitoring the temperature inside the casing 100, and the temperature detection sensor 510 is electrically connected to the temperature control circuit board 500, and can feed back the temperature condition inside the casing 100 to the temperature control circuit board 500 so as to adjust the peltier 300 to control the temperature inside the casing 100.
In this embodiment, a temperature protection switch 520 is further included, the temperature protection switch 520 is disposed on the temperature-controlled circuit board 500, and the temperature protection switch 520 is used for performing power-off protection when the temperature inside the casing 100 exceeds a process set value, so as to avoid damaging the PCR amplification process due to too high or too low temperature.
In the present embodiment, the circulation fan 400 has a plurality of circulation fans 400, and the plurality of circulation fans 400 are disposed around the peltier 300, and the rate of temperature change in the casing 100 can be increased by providing the plurality of circulation fans 400.
In this embodiment, the sample loading module 200 includes a plurality of sample wells 210 and a plurality of gene chips 220, the gene chips 220 are disposed in the sample wells 210, the sample wells 210 are uniformly distributed on the sample loading module 200, and the number of the gene chips 220 corresponds to the number of the sample wells 210, so that the sample is placed on the gene chips 220 during amplification.
In this embodiment, a guide edge is provided in the sample well 210, and the guide edge is used to limit the movement of the gene chip 220.
In this embodiment, the heat dissipation fan 110 located below the peltier 300 is disposed in the casing 100, specifically, the heat dissipation fan 110 is located at the bottom of the casing 100, and is capable of discharging heat generated in the working process of the temperature control circuit board 500 and the circulation fan 400, so as to achieve the purpose of dissipating heat from the temperature control circuit board 500 and the circulation fan 400, and thus, the work of the heat dissipation fan is more stable.
In this embodiment, heat dissipation holes 120 for discharging heat inside the casing 100 are formed on two side walls of the casing 100, and a transparent window 130 for observing the sample loading module 200 is formed on the top of the casing 100.
In this embodiment, the maximum temperature rise rate in the casing 100 is 10 ℃/s, and the maximum temperature drop rate in the casing 100 is 8 ℃/s.
The utility model discloses a theory of operation:
the sample to be amplified is placed on the gene chip 220 in the sample groove 210, the housing 100 is in a closed state, the peltier 300 is in an energized state through the temperature control circuit board 500, so that the peltier 300 is heated or cooled, and the temperature detection sensor 510 can monitor the temperature in the housing 100 in real time and feed back the temperature to the temperature control circuit board 500, so that the PCR sample can be amplified. The temperature control circuit board 500, the temperature detection sensor 510 and the temperature protection switch 520 are mature existing devices, and can be applied to products on the market.
The above description is only for the specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art can easily think of the changes or substitutions within the technical scope of the present invention, and all should be covered within the protection scope of the present invention.
Claims (10)
1. The PCR amplification instrument is characterized by comprising a shell (100), a sample loading module (200), a Peltier (300) and a circulating fan (400), wherein the sample loading module (200) is arranged in the shell (100), the sample loading module (200) is used for accommodating a sample, the Peltier (300) is arranged below the sample loading module (200), the Peltier (300) can be heated or cooled after being electrified, and the circulating fan (400) is arranged on one side of the Peltier (300) so that the circulating fan (400) can blow air cooled or heated by the Peltier (300) to the sample loading module (200).
2. The PCR amplifier of claim 1, further comprising a temperature controlled circuit board (500), wherein the temperature controlled circuit board (500) is disposed below the peltier (300), the peltier (300) is mounted on the temperature controlled circuit board (500) and the temperature controlled circuit board (500) provides electrical energy to the peltier (300).
3. The PCR amplifier of claim 2, further comprising a temperature detection sensor (510), the temperature detection sensor (510) being disposed on the temperature-controlled circuit board (500), the temperature detection sensor (510) being configured to monitor a temperature within the housing (100).
4. The PCR amplification apparatus of claim 2, further comprising a temperature protection switch (520), wherein the temperature protection switch (520) is disposed on the temperature-controlled circuit board (500), and the temperature protection switch (520) is used to protect the power supply when the temperature inside the housing (100) exceeds a set value of the process.
5. The PCR amplification apparatus according to claim 1, wherein the circulation fan (400) has a plurality of circulation fans (400), and the plurality of circulation fans (400) are disposed around the Peltier (300).
6. The PCR amplification apparatus of claim 1, wherein the sample loading module (200) comprises a sample well (210) and a gene chip (220), the gene chip (220) being disposed within the sample well (210).
7. The PCR amplification apparatus of claim 6, wherein a guide edge is disposed in the sample chamber (210), and the guide edge is used to limit the movement of the gene chip (220).
8. The PCR amplification apparatus of claim 1, wherein a heat dissipation fan (110) is disposed in the housing (100) below the Peltier (300).
9. The PCR amplification apparatus of claim 1, wherein two side walls of the housing (100) are provided with heat dissipation holes (120) for discharging heat in the housing (100), and a transparent window (130) for observing the sample loading module (200) is disposed at the top of the housing (100).
10. The PCR amplifier of claim 1, wherein the maximum temperature rise rate in the housing (100) is 10 ℃/s, and the maximum temperature fall rate in the housing (100) is 8 ℃/s.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021271346.2U CN213012879U (en) | 2020-07-01 | 2020-07-01 | PCR augmentor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021271346.2U CN213012879U (en) | 2020-07-01 | 2020-07-01 | PCR augmentor |
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| Publication Number | Publication Date |
|---|---|
| CN213012879U true CN213012879U (en) | 2021-04-20 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202021271346.2U Active CN213012879U (en) | 2020-07-01 | 2020-07-01 | PCR augmentor |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116574600A (en) * | 2023-05-25 | 2023-08-11 | 山东东大检测科技有限公司 | PCR detection equipment |
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2020
- 2020-07-01 CN CN202021271346.2U patent/CN213012879U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116574600A (en) * | 2023-05-25 | 2023-08-11 | 山东东大检测科技有限公司 | PCR detection equipment |
| CN116574600B (en) * | 2023-05-25 | 2023-11-17 | 山东东大检测科技有限公司 | PCR detection equipment |
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