CN109666585A - A kind of nucleic acid augmentative instrument - Google Patents
A kind of nucleic acid augmentative instrument Download PDFInfo
- Publication number
- CN109666585A CN109666585A CN201910011371.2A CN201910011371A CN109666585A CN 109666585 A CN109666585 A CN 109666585A CN 201910011371 A CN201910011371 A CN 201910011371A CN 109666585 A CN109666585 A CN 109666585A
- Authority
- CN
- China
- Prior art keywords
- instrument
- nucleic acid
- control system
- instrument body
- electrically connected
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 22
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 22
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 22
- 230000003190 augmentative effect Effects 0.000 title claims abstract description 20
- 238000010438 heat treatment Methods 0.000 claims abstract description 43
- 238000009413 insulation Methods 0.000 claims description 6
- 239000002184 metal Substances 0.000 claims description 4
- 230000005855 radiation Effects 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract description 8
- 238000010586 diagram Methods 0.000 description 3
- 230000003321 amplification Effects 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
- 230000005611 electricity Effects 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000017525 heat dissipation Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of nucleic acid augmentative instruments, feature be include instrument body, amplified reaction chamber and the lid for closing amplified reaction chamber closing cap are provided in instrument body, lid is arranged on instrument body turnablely, the control system for being provided with the heating mechanism for providing reaction temperature to amplified reaction chamber in instrument body and being heated for controlling heating mechanism, heating mechanism is electrically connected to the control system, the rechargeable battery for powering to control system is provided in instrument body, rechargeable battery is electrically connected to the control system, charging interface is provided on instrument body, charging interface is electrically connected with rechargeable battery.Advantage is that the volume of entire nucleic acid augmentative instrument is relatively small, is convenient for carrying, and is suitable for directly using at detection scene.
Description
Technical field
The present invention relates to technical field of molecular biological detection more particularly to a kind of nucleic acid augmentative instruments.
Background technique
Molecular biology Fast Detection Technique medical diagnosis on disease, in terms of playing the work to become more and more important
With nucleic acid amplification detection is exactly one such detection mode the most conventional, and the detection side being most widely used at present
One of formula.Nucleic acid augmentative instrument is a kind of instrument for largely synthesize in vitro to specific gene order using archaeal dna polymerase, is
One of the laboratory apparatus of molecular biology routine.Current existing nucleic acid augmentative instrument is needed through 220V alternating current come real
It now powers, this makes the instrument need to use in fixed place, greatly limits the application that this technology detects at the scene.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of compact, be convenient for carrying, be suitable for detection scene into
The nucleic acid augmentative instrument of enforcement.
The technical scheme of the invention to solve the technical problem is:
A kind of nucleic acid augmentative instrument, including instrument body are provided with amplified reaction chamber in the instrument body and for will be described
The lid that closes of amplified reaction chamber closing cap, the lid is arranged in turnablely on the instrument body, the instrument
The heating mechanism for providing reaction temperature to the amplified reaction chamber is provided in device ontology and for controlling described add
The control system of hot mechanism heats, the heating mechanism are electrically connected with the control system, are set in the instrument body
It is equipped with the rechargeable battery for powering to the control system, the rechargeable battery is electrically connected with the control system
It connects, charging interface is provided on the instrument body, the charging interface is electrically connected with the rechargeable battery.
The amplified reaction is intracavitary to be provided with reaction block, is provided on the reaction block multiple for grafting PCR pipe
Spliced eye.It is used to accept the PCR pipe equipped with biological sample by reaction block, wherein the size and spliced eye of the PCR pipe selected
It is adapted, so that reaction can be carried out preferably.
The heating mechanism includes heating plate, and the heating plate is electrically connected with the control system, and described adds
The lower section of the reaction block is arranged in hot plate.Above-mentioned heating mechanism structure is simple, controls heating plate fever by control system,
It is used to provide reaction temperature to reaction block by heating plate.
Be provided with heat-conducting plate between the heating plate and the reaction block, the upper surface of the heat-conducting plate with it is described
Reaction block lower end surface be bonded setting, the lower end surface of the heat-conducting plate is bonded setting with the upper surface of the heating plate.
By the setting of heat-conducting plate the heat generated in heating plate is preferably transmitted on reaction block, so that reaction effect
Rate is improved.
Thermal insulation board is provided with below the heating plate, the bottom plate of the instrument body is arranged in the thermal insulation board
On.Instrument body is formed by thermal insulation board and is protected, the high temperature for avoiding heating plate from generating impacts instrument body.
It is provided with radiator fan in the lid, is respectively arranged with position on the upper surface and lower end surface of the lid
Corresponding upper air outlet and lower air outlet, the radiator fan be arranged in the upper air outlet and the lower air outlet it
Between, two sides of amplified reaction chamber described in the lower air outlet face, the lid are respectively arranged with air inlet, institute
The radiator fan stated is electrically connected with the control system.Radiator fan carries out rapid cooling to instrument body after being used to react,
To effectively extend the service life of entire instrument, two air inlets of lid side are set for air draught, upper air outlet and
Lower air outlet is used for outlet air, and starting and stopping for radiator fan is controlled by control system.
Touch-sensitive display screen, the touch-sensitive display screen and the control system are provided on the instrument body
Electrical connection.By touch-sensitive display screen for manipulating control system.
The reaction block is heat-conducting metal.Reaction block is heat-conducting metal, so that heat transfer is more efficient, thus effectively
Improve reaction efficiency.
One end of the lid is rotatably arranged on the instrument body.
The amplified reaction is intracavitary to be provided with temperature sensor, the temperature sensor and control system electricity
Connection.Be used to detect temperature in reaction chamber by temperature sensor, feed back to control system so that heating plate be capable of providing compared with
For suitable reaction temperature.
Power switch is provided on the instrument body, the power switch is electrically connected with the control system.
On-off of the power switch for entire instrument is electrically operated.
Compared with the prior art, the advantages of the present invention are as follows: the amplified reaction chamber being arranged in instrument body is used for biological sample
This amplified reaction, heating mechanism are used to provide suitable reaction temperature to reaction, and control system adds for controlling heating mechanism
Heat, rechargeable battery are used to power to control system, and charging interface is used to charge to rechargeable battery, so that entire nucleic acid amplification
The volume of instrument is relatively small, is convenient for carrying, and is suitable for directly using at detection scene.
Detailed description of the invention
Fig. 1 is overall structure diagram of the invention；
Fig. 2 is the structural schematic diagram under lid opening state of the present invention；
Fig. 3 is the structural schematic diagram that Fig. 2 removes reaction block；
Fig. 4 is schematic cross-sectional view of the invention.
Specific embodiment
The present invention will be described in further detail below with reference to the embodiments of the drawings.
As shown in Figures 1 to 4, a kind of nucleic acid augmentative instrument, including instrument body 1 are provided with amplified reaction in instrument body 1
Chamber 11 and lid 12 for closing 11 closing cap of amplified reaction chamber, lid 12 are arranged in turnablely on instrument body 1, instrument
The heating mechanism for providing reaction temperature to amplified reaction chamber 11 is provided in ontology 1 and for controlling heating mechanism heating
Control system 9, heating mechanism are electrically connected with control system 9, be provided in instrument body 1 for control system 9 power can
Rechargeable battery 2, rechargeable battery 2 are electrically connected with control system 9, and charging interface 13, charging interface 13 are provided on instrument body 1
It is electrically connected with rechargeable battery 2.
In this particular embodiment, it is provided with reaction block 3 in amplified reaction chamber 11, multiple be used for is provided on reaction block 3
The spliced eye 31 of grafting PCR pipe.
In this particular embodiment, heating mechanism includes heating plate 4, and heating plate 4 is electrically connected with control system 9, heating plate 4
The lower section of reaction block 3 is set.
In this particular embodiment, be provided with heat-conducting plate 5 between heating plate 4 and reaction block 3, the upper surface of heat-conducting plate 5 with
The lower end surface of reaction block 3 is bonded setting, and the lower end surface of heat-conducting plate 5 is bonded setting with the upper surface of heating plate 4.
In this particular embodiment, the lower section of heating plate 4 is provided with thermal insulation board 6, and instrument body 1 is arranged in thermal insulation board 6
On bottom plate.
In this particular embodiment, it is provided with radiator fan 7 in lid 12, distinguishes on the upper surface and lower end surface of lid 12
It is provided with the corresponding upper air outlet 121 in position and lower air outlet 122, radiator fan 7 is arranged in upper air outlet 121 and lower air outlet
Between 122, lower 122 face amplified reaction chamber 11 of air outlet, two sides of lid 12 are respectively arranged with air inlet 123, heat dissipation
Fan 7 is electrically connected with control system 9.
In this particular embodiment, touch-sensitive display screen 8 is provided on instrument body 1, touch-sensitive display screen 8 and control are
9 electrical connection of system.
In this particular embodiment, reaction block 3 is heat-conducting metal.
In this particular embodiment, one end of lid 12 is rotatably arranged on instrument body 1.
In this particular embodiment, temperature sensor (not shown), temperature sensing are provided in amplified reaction chamber 11
Device is electrically connected with control system 9.
In this particular embodiment, power switch 14,9 electricity of power switch 14 and control system are provided on instrument body 1
Connection.
In this particular embodiment, control system 9 is conventional control circuit, be mainly used for controlling the heating of heating plate 4,
The the opening or closing of radiator fan 7, on-off of power supply etc..
Claims (10)
1. a kind of nucleic acid augmentative instrument, it is characterised in that including instrument body, amplified reaction chamber is provided in the instrument body
With the lid for closing the amplified reaction chamber closing cap, the instrument body is arranged in the lid turnablely
On, it is provided in the instrument body for providing the heating mechanism of reaction temperature to the amplified reaction chamber and for controlling
The control system of the system heating mechanism heating, the heating mechanism are electrically connected with the control system, the instrument
The rechargeable battery for powering to the control system, the rechargeable battery and the control are provided in device ontology
System processed is electrically connected, and charging interface, the charging interface and the rechargeable battery are provided on the instrument body
Electrical connection.
2. a kind of nucleic acid augmentative instrument as described in claim 1, it is characterised in that the amplified reaction is intracavitary to be provided with reaction
Block is provided with multiple spliced eyes for grafting PCR pipe on the reaction block.
3. a kind of nucleic acid augmentative instrument as claimed in claim 2, it is characterised in that the heating mechanism includes heating plate, described
Heating plate be electrically connected with the control system, the lower section of the reaction block is arranged in the heating plate.
4. a kind of nucleic acid augmentative instrument as claimed in claim 3, it is characterised in that the heating plate and the reaction block it
Between be provided with heat-conducting plate, the upper surface of the heat-conducting plate is bonded setting with the lower end surface of the reaction block, and described is thermally conductive
The lower end surface of plate is bonded setting with the upper surface of the heating plate.
5. a kind of nucleic acid augmentative instrument as claimed in claim 3, it is characterised in that be provided with below the heating plate heat-insulated
Plate, the thermal insulation board are arranged on the bottom plate of the instrument body.
6. a kind of nucleic acid augmentative instrument as described in claim 1, it is characterised in that be provided with radiator fan, institute in the lid
The corresponding upper air outlet in position and lower air outlet, the radiation air are respectively arranged on the upper surface and lower end surface of the lid stated
Fan is arranged between the upper air outlet and the lower air outlet, amplified reaction described in the lower air outlet face
Two sides of chamber, the lid are respectively arranged with air inlet, and the radiator fan is electrically connected with the control system.
7. a kind of nucleic acid augmentative instrument as described in claim 1, it is characterised in that be provided with touch on the instrument body
Display screen, the touch-sensitive display screen are electrically connected with the control system.
8. a kind of nucleic acid augmentative instrument as claimed in claim 2, it is characterised in that the reaction block is heat-conducting metal.
9. a kind of nucleic acid augmentative instrument as described in claim 1, it is characterised in that one end of the lid can be rotatably set
On the instrument body.
10. a kind of nucleic acid augmentative instrument as described in claim 1, it is characterised in that the amplified reaction is intracavitary to be provided with temperature
Sensor, the temperature sensor are electrically connected with the control system.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910011371.2A CN109666585A (en) | 2019-01-07 | 2019-01-07 | A kind of nucleic acid augmentative instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910011371.2A CN109666585A (en) | 2019-01-07 | 2019-01-07 | A kind of nucleic acid augmentative instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109666585A true CN109666585A (en) | 2019-04-23 |
Family
ID=66149248
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910011371.2A Pending CN109666585A (en) | 2019-01-07 | 2019-01-07 | A kind of nucleic acid augmentative instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109666585A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110791422A (en) * | 2019-11-08 | 2020-02-14 | 宁波胤瑞生物医学仪器有限责任公司 | Regulating and controlling method of nucleic acid amplification instrument |
| CN114350760A (en) * | 2022-01-12 | 2022-04-15 | 浙江正合谷生物科技有限公司 | PCR amplification instrument and method for nucleic acid on-site temperature-variable amplification |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202530071U (en) * | 2012-02-14 | 2012-11-14 | 宁波青华科教仪器有限公司 | Gene amplification instrument |
| US20170157613A1 (en) * | 2014-05-21 | 2017-06-08 | Coyote Bioscience Co., Ltd. | Systems and methods for thermal cycling |
| CN108531390A (en) * | 2018-04-11 | 2018-09-14 | 张薇 | A kind of portable equipment that can be used for Site Detection nucleic acid |
-
2019
- 2019-01-07 CN CN201910011371.2A patent/CN109666585A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202530071U (en) * | 2012-02-14 | 2012-11-14 | 宁波青华科教仪器有限公司 | Gene amplification instrument |
| US20170157613A1 (en) * | 2014-05-21 | 2017-06-08 | Coyote Bioscience Co., Ltd. | Systems and methods for thermal cycling |
| CN108531390A (en) * | 2018-04-11 | 2018-09-14 | 张薇 | A kind of portable equipment that can be used for Site Detection nucleic acid |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110791422A (en) * | 2019-11-08 | 2020-02-14 | 宁波胤瑞生物医学仪器有限责任公司 | Regulating and controlling method of nucleic acid amplification instrument |
| CN114350760A (en) * | 2022-01-12 | 2022-04-15 | 浙江正合谷生物科技有限公司 | PCR amplification instrument and method for nucleic acid on-site temperature-variable amplification |
| CN114350760B (en) * | 2022-01-12 | 2024-04-12 | 浙江正合谷生物科技有限公司 | PCR amplification instrument and amplification method for on-site variable-temperature amplification of nucleic acid |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109666585A (en) | A kind of nucleic acid augmentative instrument | |
| KR101949829B1 (en) | Thermal cycler | |
| TW201723168A (en) | Apparatus and methods for conducting chemical reactions | |
| WO2009014139A1 (en) | Tissue piece treating apparatus | |
| CN108636471A (en) | A kind of nucleic acid amplifier and its application | |
| WO2015176674A1 (en) | Systems and methods for thermal cycling cross-reference | |
| CN106299543A (en) | A kind of graphene battery group heat management system | |
| CN115074236A (en) | Temperature control device for PCR instrument, amplification equipment and PCR instrument | |
| CN112779150A (en) | Constant temperature amplification nucleic acid detector | |
| CN107051598A (en) | PCR micro-fluidic chips and its preparation and application method and PCR equipment | |
| CN107109334B (en) | Apparatus and method for carrying out chemical reactions | |
| CN204939449U (en) | The portable PCR instrument of a kind of new microization | |
| CN210656874U (en) | Fluorescent quantitative pcr instrument | |
| CN106299503B (en) | A kind of uniform regulator control system of temperature of powered cell | |
| US11913649B2 (en) | Cooking appliance | |
| CN209778874U (en) | Nucleic acid amplification instrument | |
| CN216385071U (en) | Vacuum smelting furnace with waste heat recovery device | |
| TWI578595B (en) | Battery device and thermal control method thereof | |
| CN201909532U (en) | Debinding furnace | |
| CN101735949A (en) | Temperature change device | |
| CN208494282U (en) | Low-temperature metal bath apparatus | |
| CN217418692U (en) | PCR detection system | |
| CN216764929U (en) | Nucleic acid extraction amplification detection kit | |
| CN103047765B (en) | A kind of boiler with temp controlled thermal insulation function | |
| CN108531390A (en) | A kind of portable equipment that can be used for Site Detection nucleic acid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20210125 Address after: 315040 Ningbo New Materials Innovation Center East District, Ningbo High-tech Zone, Zhejiang Province, No. 1 11-2-1 Applicant after: Zhejiang Zhenghegu Biotechnology Co.,Ltd. Address before: 315211, Fenghua Road, Jiangbei District, Zhejiang, Ningbo 818 Applicant before: Ningbo University |
|
| TA01 | Transfer of patent application right |