CN108531390A - A kind of portable equipment that can be used for Site Detection nucleic acid - Google Patents
A kind of portable equipment that can be used for Site Detection nucleic acid Download PDFInfo
- Publication number
- CN108531390A CN108531390A CN201810319744.8A CN201810319744A CN108531390A CN 108531390 A CN108531390 A CN 108531390A CN 201810319744 A CN201810319744 A CN 201810319744A CN 108531390 A CN108531390 A CN 108531390A
- Authority
- CN
- China
- Prior art keywords
- heating module
- storage tank
- nucleic acid
- upper cover
- foam
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 41
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 35
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 34
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 34
- 238000010438 heat treatment Methods 0.000 claims abstract description 54
- 229920001821 foam rubber Polymers 0.000 claims abstract description 17
- -1 apparatus body Chemical class 0.000 claims abstract description 4
- 239000003153 chemical reaction reagent Substances 0.000 claims description 11
- 239000004065 semiconductor Substances 0.000 claims description 3
- 230000017525 heat dissipation Effects 0.000 claims 1
- 230000003321 amplification Effects 0.000 abstract description 16
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 16
- 238000000034 method Methods 0.000 abstract description 4
- 230000005284 excitation Effects 0.000 abstract description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000005611 electricity Effects 0.000 description 3
- 238000009413 insulation Methods 0.000 description 3
- 230000003190 augmentative effect Effects 0.000 description 2
- 239000000428 dust Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003032 phytopathogenic effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
Abstract
The invention discloses a kind of portable equipment that can be used for Site Detection nucleic acid, including apparatus body, the bottom end of apparatus body is provided with storage tank, upper cover is provided at the top of apparatus body, support column is installed between upper cover and storage tank, supporting plate is provided at the top of support column, the bottom of support column is provided with fixed seat, fixed seating is in groove, the bottom end of upper cover is provided with link block, the top of storage tank is equipped with foam-rubber cushion, several placing grooves are provided on foam-rubber cushion, one end of placing groove is provided with card slot, the inside of placing groove is equipped with the first heating module and the second heating module.Structure of the invention is novel, easy to operate, this equipment application nucleic acid amplification and the principle of detection have carried out the detection work of paired samples, the fluorescence excitation and collection used when the temperature for calculating amplification procedure is controlled and detected integrates, and realizes the miniaturization of equipment；Simplify equipment when in use equipped with touch screen operation；Chargeable internal battery.
Description
Technical field
The present invention relates to detection devices, and in particular to a kind of portable equipment that can be used for Site Detection nucleic acid.
Background technology
Currently, nucleic acid amplification and detection are the standardization programs often carried out in scientific research and laboratory.Many standard detections
Amplification and the relevant nucleic acid of detection are relied primarily on to diagnose the illness.For example, relying on pathogenic microorganisms or dynamic in detection of nucleic acids food
Phytopathogenic microorganism etc. is in order not to same purpose, and promotes ease, efficiency, specificity or the reduction of nucleic acid amplification or detection
Cost, it is a large amount of to be developed suitable for nucleic acid amplification and the method and device of detection.Such as rely on PCR (PCR)
Nucleic acid amplification detection method.It is expensive since traditional nucleic acid augmentative instrument such as PCR instrument is bulky, may not all users can
With burden.In order to be more convenient and be simply completed nucleic acid amplification and detection, such as portable nucleic acid augmentative instrument, miniature PCR amplification
(pcr chip), constant-temperature amplification etc. have been developed that there are many currently marketed detection of nucleic acids unit type, nucleic acid amplification
Temperature control mode has air heating, semiconductor heating etc..But the overwhelming majority is the supply for relying on extraneous power supply and completing energy, this
It limits detection of nucleic acids equipment and is only capable of laboratory environment in condition abundance, it cannot should be in field, open country by detection of nucleic acids equipment
Realize detection in outer equal scene.This proposes detection of nucleic acids equipment new requirement.
Invention content
The technical problem to be solved by the present invention is to overcome the existing detection of nucleic acids equipment overwhelming majority be to rely on extraneous electricity
The supply of energy is completed in source, and which also limits detection of nucleic acids equipment to be only capable of the laboratory environment in condition abundance, cannot be by nucleic acid
Detection device should provide the problem of realizing detection at the scene such as field, field and a kind of portable can be used for Site Detection nucleic acid
Equipment.
In order to solve the above technical problem, the present invention provides the following technical solutions：
The present invention provides a kind of portable equipment that can be used for Site Detection nucleic acid, including apparatus body, the equipment
The bottom end of ontology is provided with storage tank, upper cover is provided at the top of the apparatus body, between the upper cover and the storage tank
Support column is installed, supporting plate is provided at the top of the support column, the bottom of the support column is provided with fixed seat, the fixation
Seat is located in groove, and the bottom end of the upper cover is provided with link block, and the top of the storage tank is equipped with foam-rubber cushion, the sponge
Several placing grooves are provided on pad, one end of the placing groove is provided with card slot, and the inside of the placing groove is equipped with first and adds
It is provided with eight through holes, institute on thermal modules and the second heating module, first heating module and second heating module
The both ends for stating the first heating module and second heating module are mounted on heat emission fan, and the outer wall setting of the placing groove is limited
Position block, the inner wall of the limited block are equipped with several LED light, and the bottom end of the placing groove is equipped with fluorescence detector, the storage
The bottom of object case is equipped with accumulator, and control circuit board, the storage are equipped between the accumulator and the fluorescence detector
The both ends and bottom end of battery are mounted on heat sink, and one end of the storage tank is provided with several USB interfaces, the USB interface
Touch screen is connected by USB wiring, the other end of the storage tank is equipped with handle, between the foam-rubber cushion and the storage tank
It is provided with link slot.
As a preferred technical solution of the present invention, the output end of the touch screen connects the control electricity with input terminal
Road plate, the output end of the control circuit board connect first heating module, second heating module, described with input terminal
LED light and fluorescence detector.
As a preferred technical solution of the present invention, first heating module and second heating module pass through institute
It states placing groove and connects the foam-rubber cushion, the support column connects the upper cover by the supporting plate, and the support column passes through described
Fixed seat connects the foam-rubber cushion with the groove.
As a preferred technical solution of the present invention, the upper cover connects the link slot, institute by the link block
The inside for stating through hole is equipped with Reagent Tube.
As a preferred technical solution of the present invention, first heating module and second heating module are partly to lead
Body heating module.
The advantageous effect that is reached of the present invention is：Structure of the invention is novel, easy to operate, this equipment application nucleic acid amplification
The detection work for having carried out paired samples with the principle of detection, the fluorescence used when the temperature for calculating amplification procedure is controlled and detected
Excitation and collection integrate, and realize the miniaturization of equipment；Simplify equipment when in use equipped with touch screen operation；It can
The internal battery of charging, which is equipment, can be detached from laboratory environment, break through the limitation of extraneous power supply, be that equipment is truly realized
Site Detection completes the amplification to nucleic acid and real-time result monitoring work.
Description of the drawings
Attached drawing is used to provide further understanding of the present invention, and a part for constitution instruction, the reality with the present invention
It applies example to be used to explain the present invention together, not be construed as limiting the invention.In the accompanying drawings：
Fig. 1 is the structural schematic diagram of the present invention；
Fig. 2 is the storage box structure schematic diagram of the present invention；
Fig. 3 is the control module structural schematic diagram of the present invention.
In figure：1, apparatus body；2, upper cover；3, link block；4, supporting plate；5, support column；6, fixed seat；7, USB interface；8、
USB wiring；9, touch screen；10, storage tank；11, accumulator；12, fluorescence detector；13, control circuit board；14, heat sink；
15, heat emission fan；16, through hole；17, the first heating module；18, LED light；19, the second heating module；20, placing groove；21, it limits
Position block；22, card slot；23, groove；24, handle；25, link slot；26, foam-rubber cushion.
Specific implementation mode
Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings, it should be understood that preferred reality described herein
Apply example only for the purpose of illustrating and explaining the present invention and is not intended to limit the present invention.
Embodiment 1
As shown in Figs. 1-3, the present invention provides a kind of portable equipment that can be used for Site Detection nucleic acid, including apparatus body
1, the bottom end of apparatus body 1 is provided with storage tank 10, and the top of apparatus body 1 is provided with upper cover 2, upper cover 2 and storage tank 10 it
Between support column 5 is installed, the top of support column 5 is provided with supporting plate 4, and the bottom of support column 5 is provided with fixed seat 6, fixed seat 6
In in groove 23, the bottom end of upper cover 2 is provided with link block 3, and the top of storage tank 10 is equipped with foam-rubber cushion 26, is set on foam-rubber cushion 26
Several placing grooves 20 are equipped with, one end of placing groove 20 is provided with card slot 22, and the inside of placing groove 20 is equipped with the first heating module
17 and second heating module 19, be provided with eight through holes 16 on the first heating module 17 and the second heating module 19, first plus
The both ends of thermal modules 17 and the second heating module 19 are mounted on heat emission fan 15, and the outer wall of placing groove 20 is provided with limited block 21,
The inner wall of limited block 21 is equipped with several LED light 18, and the bottom end of placing groove 20 is equipped with fluorescence detector 12, the bottom of storage tank 10
Portion is equipped with accumulator 11, and control circuit board 13, the both ends of accumulator 11 are equipped between accumulator 11 and fluorescence detector 12
It is mounted on heat sink 14 with bottom end, one end of storage tank 10 is provided with several USB interfaces 7, and USB interface 7 passes through USB wiring 8
Touch screen 9 is connected, the other end of storage tank 10 is equipped with handle 24, link slot is provided between foam-rubber cushion 26 and storage tank 10
25。
The output end of touch screen 9 connects control circuit board 13, the output end and input terminal of control circuit board 13 with input terminal
The first heating module 17, the second heating module 19, LED light 18 and fluorescence detector 12 are connected, is conducive to touch screen 9 and passes through control
Circuit board 13 controls the first heating module 17, the second heating module 19, LED light 18 and fluorescence detector 12.
First heating module 17 and the second heating module 19 connect foam-rubber cushion 26, the first heating module 17 by placing groove 20
With the second heating module 19 for being heated to reagent in Reagent Tube, heat insulation function, support column 5 connects upper cover 2, branch by supporting plate 4
Dagger 5 connects foam-rubber cushion 26 by fixed seat 6 and groove 23, shuts out the light convenient for upper cover 2, blocks extraneous dust and foreign matter,
With certain heat insulation function.
Upper cover 2 connects link slot 25 by link block 3, and the inside of through hole 16 is equipped with Reagent Tube.
First heating module 17 and the second heating module 19 are semiconductor heating module.
The device is a kind of portable equipment that can be used for Site Detection nucleic acid, and apparatus body 1 opens upper cover 2 first, it
Rear support strut 6 is fixed on by fixed seat 6 in groove 23 by 4 fixed cover 2 of supporting plate, support column 6, is convenient for 2 shielding light of upper cover
Line blocks extraneous dust and foreign matter, has certain heat insulation function, places reagent in Reagent Tube again later, later USB interface
7 connect touch screen 9 by USB wiring 8, have 8 through holes 16 on the first heating module 17 and the second heating module 19, are used for
Reagent Tube is placed, the first heating module 17 is controlled by touch screen 9 and the second heating module 19 heats reagent in Reagent Tube, simultaneously
It is 470nm light to be used for launch wavelength by the LED light 18 of bottom, excites reagent fluorescence in sample cell, and bottom also has identification
The fluorescence detector 12 of the light of wavelength 510nm or so is detected, while detection data is delivered to touch screen 9, control electricity
Road plate 13 is loaded with operating system, the data and offer apparatus body 1 for processing detection and the extraneous USB interface 7 connecting,
It is connect with touch screen 9, receives the signal sent out on touch screen 9, control the display content on touch screen 9, the first heating of control control
Module 17, the second heating module 19, LED light 18 and fluorescence detector 12, by heat emission fan 15 to the first heating module 17 and second
Heating module 19 carries out radiating treatment, carries out radiating treatment to storage tank 10 by cooling fin 14, accumulator 11 is whole equipment sheet
1 supplying energy of body, and can be charged repeatedly by USB interface 7, every time after charging, equipment operation is not less than 4 hours, does not make
Used time lays down support column, is placed in card slot 22, and lid 2 connects link slot 25 by link block 3, and storage tank is closed by upper cover 2
10, it is carried by handle 24.
The advantageous effect that is reached of the present invention is：Structure of the invention is novel, easy to operate, this equipment application nucleic acid amplification
The detection work for having carried out paired samples with the principle of detection, the fluorescence used when the temperature for calculating amplification procedure is controlled and detected
Excitation and collection integrate, and realize the miniaturization of equipment；Simplify equipment when in use equipped with touch screen operation；It can
The internal battery of charging, which is equipment, can be detached from laboratory environment, break through the limitation of extraneous power supply, be that equipment is truly realized
Site Detection completes the amplification to nucleic acid and real-time result monitoring work.
Finally it should be noted that：The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention,
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, still may be used
With technical scheme described in the above embodiments is modified or equivalent replacement of some of the technical features.
All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in the present invention's
Within protection domain.
Claims (5)
1. a kind of portable equipment that can be used for Site Detection nucleic acid, including apparatus body (1), which is characterized in that the equipment
The bottom end of ontology (1) is provided with storage tank (10), and upper cover (2), the upper cover (2) are provided at the top of the apparatus body (1)
Support column (5) is installed between the storage tank (10), supporting plate (4), the support are provided at the top of the support column (5)
The bottom of column (5) is provided with fixed seat (6), and the fixed seat (6) is located in groove (23), the bottom end setting of the upper cover (2)
There are link block (3), the top of the storage tank (10) that foam-rubber cushion (26) is installed, several put is provided on the foam-rubber cushion (26)
Slot (20) is set, one end of the placing groove (20) is provided with card slot (22), and the inside of the placing groove (20) is equipped with first and adds
Thermal modules (17) and the second heating module (19) are arranged on first heating module (17) and second heating module (19)
There are eight through holes (16), the both ends of first heating module (17) and second heating module (19) are mounted on heat dissipation
It fans (15), the outer wall of the placing groove (20) is provided with limited block (21), and the inner wall of the limited block (21) is equipped with several LED
The bottom end of lamp (18), the placing groove (20) is equipped with fluorescence detector (12), and the bottom of the storage tank (10) is equipped with storage
Battery (11) is equipped with control circuit board (13), the electric power storage between the accumulator (11) and the fluorescence detector (12)
The both ends and bottom end in pond (11) are mounted on heat sink (14), and one end of the storage tank (10) is provided with several USB interfaces
(7), the USB interface (7) connects touch screen (9) by USB wiring (8), and the other end of the storage tank (10) is equipped with handle
Hand (24) is provided with link slot (25) between the foam-rubber cushion (26) and the storage tank (10).
2. a kind of portable equipment that can be used for Site Detection nucleic acid according to claim 1, which is characterized in that described to touch
The output end for touching screen (9) connects the control circuit board (13) with input terminal, the output end of the control circuit board (13) and defeated
Enter end and connects first heating module (17), second heating module (19), the LED light (18) and fluorescence detector
(12)。
3. a kind of portable equipment that can be used for Site Detection nucleic acid according to claim 1, which is characterized in that described
One heating module (17) and second heating module (19) connect the foam-rubber cushion (26) by the placing groove (20), described
Support column (5) connects the upper cover (2) by the supporting plate (4), and the support column (5) passes through the fixed seat (6) and described
Groove (23) connects the foam-rubber cushion (26).
4. a kind of portable equipment that can be used for Site Detection nucleic acid according to claim 1, which is characterized in that on described
It covers (2) and the link slot (25) is connected by the link block (3), the inside of the through hole (16) is equipped with Reagent Tube.
5. a kind of portable equipment that can be used for Site Detection nucleic acid according to claim 1, which is characterized in that described
One heating module (17) and second heating module (19) are semiconductor heating module.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810319744.8A CN108531390A (en) | 2018-04-11 | 2018-04-11 | A kind of portable equipment that can be used for Site Detection nucleic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810319744.8A CN108531390A (en) | 2018-04-11 | 2018-04-11 | A kind of portable equipment that can be used for Site Detection nucleic acid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108531390A true CN108531390A (en) | 2018-09-14 |
Family
ID=63480827
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810319744.8A Pending CN108531390A (en) | 2018-04-11 | 2018-04-11 | A kind of portable equipment that can be used for Site Detection nucleic acid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108531390A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109666585A (en) * | 2019-01-07 | 2019-04-23 | 宁波大学 | A kind of nucleic acid augmentative instrument |
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| CN103347994A (en) * | 2010-12-03 | 2013-10-09 | 拜欧法诊断有限公司 | Thermal cycler apparatus and related methods |
| CN204714811U (en) * | 2015-02-09 | 2015-10-21 | 张国豪 | A kind of portable detection of nucleic acids equipment |
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| JP3203237U (en) * | 2016-01-07 | 2016-03-17 | サーモジェン有限会社 | Portable DNA amplification device |
| CN105765054A (en) * | 2013-10-07 | 2016-07-13 | 道格拉斯科学有限责任公司 | Portable testing device for analyzing biological samples |
| CN206646095U (en) * | 2017-03-02 | 2017-11-17 | 张薇 | One-stop site nucleic acid augmentation detection carrying case |
| CN107513490A (en) * | 2017-09-29 | 2017-12-26 | 重庆京因生物科技有限责任公司 | A kind of full-automatic medical fluorescent PCR analysis system based on POCT patterns |
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2018
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|---|---|---|---|---|
| JP2009171921A (en) * | 2008-01-28 | 2009-08-06 | Olympus Corp | Apparatus and method for detecting fluorescent amplified nucleic acid |
| CN101328503A (en) * | 2008-07-18 | 2008-12-24 | 杭州博日科技有限公司 | Fluorescent quantitative PCR detection system based on bottom scan detection |
| CN103347994A (en) * | 2010-12-03 | 2013-10-09 | 拜欧法诊断有限公司 | Thermal cycler apparatus and related methods |
| CN105765054A (en) * | 2013-10-07 | 2016-07-13 | 道格拉斯科学有限责任公司 | Portable testing device for analyzing biological samples |
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| CN109666585A (en) * | 2019-01-07 | 2019-04-23 | 宁波大学 | A kind of nucleic acid augmentative instrument |
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| PB01 | Publication | ||
| PB01 | Publication | ||
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Application publication date: 20180914 |