CN216513851U

CN216513851U - Handheld micro-fluidic device for nucleic acid detection

Info

Publication number: CN216513851U
Application number: CN202122862486.8U
Authority: CN
Inventors: 孙浩; 余勇健; 东辉
Original assignee: Fuzhou University
Current assignee: Fuzhou University
Priority date: 2021-11-19
Filing date: 2021-11-19
Publication date: 2022-05-13
Anticipated expiration: 2031-11-19

Abstract

The utility model relates to a handheld nucleic acid detection microfluidic device which comprises a device shell, an LED lamp, a band-pass filter, a ceramic heating sheet, a detection sample microfluidic chip, a long-pass filter and a camera device. The device has scientific and reasonable design, simple and compact structure, small volume, simple and convenient operation and convenient carrying.

Description

Handheld micro-fluidic device for nucleic acid detection

Technical Field

The utility model belongs to the technical field of nucleic acid detection, and particularly relates to a handheld micro-fluidic nucleic acid detection device.

Background

Infectious diseases have always posed a serious threat to public health and are a leading cause of death and disability in the world. In recent years, the types of life-threatening infectious diseases are increasing, especially new coronary pneumonia outbreaks in the world, and the pandemic in the global range causes huge loss to the whole world due to strong infectivity and high morbidity.

The nucleic acid detection method has been one of the gold standards for infectious disease detection because of its remarkable advantages such as excellent sensitivity and strong specificity. First, nucleic acids, a common genetic material, have unique sequences and numbers in different species of organisms. Secondly, nucleic acids are easily detected because all DNA and RNA sequences are composed of four basic nucleotides. Third, many nucleic acid sequences can now be synthesized in vitro in a short time and at low cost. Finally, with the rapid development of nucleic acid sequencing technologies, nucleic acid detection can meet the requirements of clinical applications. These accurate and sensitive nucleic acid detection results provide valid evidence for medical diagnosis. Particularly, POCT (Point-of-caretest) on-site rapid nucleic acid detection has been increasingly paid close attention to people due to its remarkable advantages of short diagnosis time, high automation level, simple operation and the like.

Disclosure of Invention

The utility model aims to provide a handheld nucleic acid detection microfluidic device which is scientific and reasonable in design, simple and compact in structure, small in size, simple and convenient to operate and convenient to carry.

In order to achieve the purpose, the utility model adopts the technical scheme that: the utility model provides a handheld nucleic acid detection micro-fluidic device, its characterized in that includes device casing, LED lamp, band pass filter, ceramic heating piece, detection sample micro-fluidic chip, long pass filter and camera device, the device adopts parallel type light path design, sets gradually LED lamp, band pass filter, ceramic heating piece, detection sample micro-fluidic chip, long pass filter, camera device in the device casing along vertical direction.

Furthermore, the ceramic heating plate is of a hollow structure with a central through hole, so that light rays emitted by the LED lamp can pass through the ceramic heating plate while the micro-fluidic chip for detecting the sample is heated.

Further, the device shell comprises a main shell, a top sealing cover and an optical filter mounting rack, wherein a step through hole with a step table top is vertically formed in the middle of the main shell, the optical filter mounting rack is arranged on a first step surface in the step through hole to mount the long-pass optical filter, the ceramic heating plate is mounted on a second step surface on the lower side of the first step surface, the band-pass optical filter is mounted on a third step surface on the lower side of the second step surface, and the LED lamp is mounted in the through hole on the lower side of the third step surface; the upper end of the top seal cover is provided with a first groove, the camera device is installed in the first groove, the middle of the top seal cover is vertically provided with a first light-transmitting through hole, the lower end of the top seal cover is provided with a butt joint structure matched with the upper end of the stepped through hole of the main shell body, and the butt joint structure is in close butt joint fit with the stepped through hole of the main shell body.

Furthermore, a first channel is further formed in the main shell, one end of the first channel is communicated with the second stepped surface, and the other end of the first channel is communicated with the bottom or the side part of the main shell, so that a connection wire of the ceramic heating plate is led out, and the ceramic heating plate is subjected to energization heating control; and a second channel is further formed in the main shell, one end of the second channel is communicated with the through hole on the lower side of the third step surface, and the other end of the second channel is communicated with the bottom or the side part of the main shell so as to lead out the wiring of the LED lamp and further carry out power-on control on the LED lamp.

Furthermore, a second groove is formed in the upper end of the optical filter mounting frame, the long-pass optical filter is installed in the second groove, and a second light-transmitting through hole is formed in the middle of the optical filter mounting frame in the vertical direction.

Further, be equipped with control module, power module and mutual display module on the device, control module respectively with LED lamp, ceramic heating plate, camera device and mutual display module electric connection to control LED lamp, ceramic heating plate, camera device work, and carry out human-computer interaction and relevant information display through mutual display module.

Compared with the prior art, the utility model has the following beneficial effects:

1. the optical path design is simplified. In a conventional fluorescence detection device, an excitation light source and a detection device for detecting a sample to be excited to emit fluorescence are arranged on the upper side of the sample, so that an excitation light path and a fluorescence light path are easy to interfere with each other, and a detection result is influenced. And the light path is generally complex in design, needs optical elements such as a spectroscope and the like, is high in cost, and is complex in detection operation. In view of this, the utility model adopts a vertical light path design, and uses the ceramic heating plate with a central through hole as a heating device, so that the sample chip can be heated and reacted at the lower part, and the excitation light source at the lower part can also irradiate the reaction area. The light path has simple and ingenious design, avoids the mutual interference between the light source and the excited fluorescence, and simplifies the detection operation.

2. Flexible manufacture, low cost and can be improved at any time according to actual conditions. This device casing adopts the hot extrusion 3d to print the manufacturing, and the consumptive material is with low costs, and the printing mode adopts network structure to pile up the material layer upon layer for device casing inside has certain space, can save material and print the time, can alleviate the weight of device again, can play thermal-insulated effect that keeps warm moreover. To the size of different light filters and ceramic heating piece and camera, can carry out reasonable design and adjustment to inner structure, the flexibility is high.

3. And the tedious operation is avoided. The device can be used as a micro-fluidic device for instant detection, and detection can be carried out without professional operators. The device is simple and convenient in assembly method, convenient in detection operation flow and capable of providing help for remote medical and health condition laggard areas.

4. Light weight, small volume, low power consumption and convenient carrying. Different from large-scale instruments of laboratory detection systems, the instrument can only carry out fixed-point detection and is difficult to carry. As a portable POC medical diagnosis micro-fluidic device, the utility model has light weight, about 170.5 g and small occupied volume, and the basal area is 55 x 55mm²Height of 120mm and total volume of 0.000363m³And is convenient to carry. Because the power consumption of the utility model is lower, the power supply device can adopt the charger to supply power, and the USB adjustable voltage which is designed and customized independently is connected with the charger to adjust the power supply voltage.

Drawings

FIG. 1 is a schematic diagram of the internal structure of an apparatus according to an embodiment of the present invention;

FIG. 2 is a schematic structural view of a main housing in an embodiment of the present invention;

FIG. 3 is a schematic view of the construction of a top closure in an embodiment of the present invention;

fig. 4 is a schematic structural diagram of a filter mounting frame in an embodiment of the utility model.

Detailed Description

The utility model is further explained below with reference to the drawings and the embodiments.

It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.

It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.

As shown in fig. 1-4, the present embodiment provides a handheld nucleic acid detection microfluidic device, which includes a device housing, an LED lamp 5 (460-. The ceramic heating sheet 3 is a hollow structure with a central through hole, so that light emitted by the LED lamp can pass through the ceramic heating sheet 3 while the micro-fluidic chip 18 for detecting a sample is heated. In the device, due to the adoption of the parallel light path design, the excitation light path is parallel to the emission light path, the excitation light is filtered by the light filter and then vertically irradiates the detection area of the chip, and the generated fluorescence is shot by the camera device for detection after the excitation light and other stray light are filtered by the emission light filter.

In this embodiment, the device housing comprises a main housing 6, a top cover and a filter mount 16. As shown in fig. 2, the vertical ladder through hole that has the ladder mesa has been seted up along main casing 6 middle part, set up on the first step face 7 in the ladder through hole light filter mounting bracket 16, in order to install long pass filter 2, install on the second step face 8 of first step face downside ceramic heater chip 3, install on the third step face 11 of second step face downside band pass filter 4, the through-hole 12 installation of third step face downside LED lamp 5. As shown in fig. 3, a square first groove 13 is formed in the upper end of the top cover, the camera device 1 is installed in the first groove 13, a first light-transmitting through hole 14 is vertically formed in the middle of the top cover to allow the camera device to well collect images, and a square butt joint structure 15 matched with the upper end of the stepped through hole of the main housing is arranged at the lower end of the top cover and is in close butt joint with the stepped through hole of the main housing 6 through the butt joint structure 15.

In addition, a first channel 9 is further formed in the main shell 6, one end of the first channel 9 is communicated with the second stepped surface 8, and the other end of the first channel 9 is communicated with the bottom or the side of the main shell 6, so that the connection wire of the ceramic heating plate 3 is led out, and the ceramic heating plate is subjected to energization heating control; still seted up second passageway 10 in the main casing body 6, second passageway 10 one end and the through-hole 12 intercommunication of third step face 11 downside, the other end and the bottom or the other side portion intercommunication of main casing body 6 to draw the wiring of LED lamp 5, and then carry out the circular telegram control to the LED lamp.

As shown in fig. 4, in order to arrange the long-pass filter above, the present apparatus is designed with a square filter mount 16 and placed on the first step surface 7 in the stepped through-hole. The upper end of the optical filter mounting rack 16 is provided with a second groove 17, the long-pass optical filter is mounted in the second groove, and the middle part of the optical filter mounting rack is vertically provided with a second light-transmitting through hole.

In this embodiment, the device casing adopts the thermal extrusion 3d printer to make, and used material is PLA, and the material is piled up to the printing mode adoption network structure successive layer to make the inside certain space that has of device casing, both save material can alleviate the weight of device again, can play thermal-insulated effect of heat preservation moreover.

The device can be powered by an external 5V lithium battery. The control module is used for controlling the input voltage to adjust the brightness of the LED lamp and the temperature of the heating sheet. The camera is connected with a computer and is matched with corresponding detection software to complete the detection function. In addition, in order to realize the automatic work control of the device and improve the portability of the device, in a preferred embodiment of the utility model, a control module, a power module and an interactive display module can be integrated on the device, and the control module is respectively electrically connected with the LED lamp, the ceramic heating plate, the camera device and the interactive display module so as to control the LED lamp, the ceramic heating plate and the camera device to work and carry out human-computer interaction and related information display through the interactive display module.

In this embodiment, the operation process of the handheld nucleic acid detection microfluidic device is as follows: firstly, taking down a top sealing cover and an optical filter mounting rack, placing a sample detection microfluidic chip right above a ceramic heating sheet, and aligning a central through hole of the ceramic heating sheet to a detection area; then, the optical filter mounting rack and the top sealing cover are installed back; then, turning on the LED lamp, and adjusting the LED lamp to proper brightness by controlling input voltage; then, electrifying the ceramic heating sheet to heat the ceramic heating sheet to a set temperature (about 65 ℃); and finally, acquiring an image of the sample microfluidic chip in real time through the camera device, transmitting the image to a computer or a control device, calculating the average gray value of pixel points in the detection area in real time, drawing a gray value curve, and further judging the detection result. If the curve is amplified within a predetermined time (e.g., 1 hour), the result is determined to be positive.

The utility model realizes a field rapid nucleic acid detection device, simplifies the light path design, and has the advantages of low cost, simple and convenient operation, convenient carrying and the like. Can realize nucleic acid detection based on LAMP nucleic acid amplification method, and can be used for rapid detection of 2019-nCoV virus which has been outbreaked around the world in recent years. The device has important reference value for relieving the 2019-nCoV detection pressure in China and improving the disease diagnosis, prevention and control level in the remote medical condition laggard areas.

The foregoing is directed to preferred embodiments of the present invention, other and further embodiments of the utility model may be devised without departing from the basic scope thereof, and the scope thereof is determined by the claims that follow. However, any simple modification, equivalent change and modification of the above embodiments according to the technical essence of the present invention are within the protection scope of the technical solution of the present invention.

Claims

1. The utility model provides a handheld nucleic acid detection micro-fluidic device, its characterized in that includes device casing, LED lamp, band pass filter, ceramic heater piece, detection sample micro-fluidic chip, long-pass filter and camera device, the device adopts parallel type light path design, sets gradually LED lamp, band pass filter, ceramic heater piece, detection sample micro-fluidic chip, long-pass filter, camera device in the device casing along vertical direction.

2. The microfluidic device for detecting nucleic acid of claim 1, wherein the ceramic heating plate is a hollow structure with a central through hole, so that light emitted from the LED lamp can pass through the ceramic heating plate while heating the microfluidic chip for detecting nucleic acid.

3. The microfluidic device for handheld nucleic acid detection according to claim 1, wherein the device housing comprises a main housing, a top cover and a filter mounting bracket, wherein a step through hole with a step table is vertically formed in the middle of the main housing, the filter mounting bracket is arranged on a first step surface in the step through hole to mount the long-pass filter, the ceramic heating sheet is mounted on a second step surface on the lower side of the first step surface, the band-pass filter is mounted on a third step surface on the lower side of the second step surface, and the LED lamp is mounted in the through hole on the lower side of the third step surface; the camera device is installed in the first groove, a first light-transmitting through hole is vertically formed in the middle of the top sealing cover, a butt joint structure matched with the upper end of the stepped through hole of the main shell is arranged at the lower end of the top sealing cover, and the butt joint structure is in close butt joint fit with the stepped through hole of the main shell.

4. The microfluidic device for detecting nucleic acid according to claim 3, wherein the main housing further comprises a first channel, one end of the first channel is communicated with the second step surface, and the other end of the first channel is communicated with the bottom or the side of the main housing, so as to lead out a connection wire of the ceramic heating plate and further control the ceramic heating plate to be heated by electricity; and a second channel is further formed in the main shell, one end of the second channel is communicated with the through hole on the lower side of the third step surface, and the other end of the second channel is communicated with the bottom or the side part of the main shell so as to lead out the wiring of the LED lamp and further carry out power-on control on the LED lamp.

5. The microfluidic device for handheld nucleic acid detection according to claim 3, wherein the optical filter mounting frame is provided with a second groove at an upper end thereof, the long-pass optical filter is mounted in the second groove, and the optical filter mounting frame is vertically provided with a second light-transmitting through hole in a middle portion thereof.

6. The microfluidic device for handheld nucleic acid detection according to claim 1, wherein the microfluidic device is provided with a control module, a power supply module and an interactive display module, the control module is electrically connected with the LED lamp, the ceramic heating sheet, the camera device and the interactive display module respectively so as to control the LED lamp, the ceramic heating sheet and the camera device to work, and the interactive display module is used for performing human-computer interaction and related information display.