CN215250876U - Real-time fluorescence quantitative PCR instrument - Google Patents

Real-time fluorescence quantitative PCR instrument Download PDF

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Publication number
CN215250876U
CN215250876U CN202120847640.1U CN202120847640U CN215250876U CN 215250876 U CN215250876 U CN 215250876U CN 202120847640 U CN202120847640 U CN 202120847640U CN 215250876 U CN215250876 U CN 215250876U
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pcr instrument
water bath
real
electric heating
main part
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CN202120847640.1U
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马晓晶
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Wuhan Healthcare Biotechnology Co ltd
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Wuhan Healthcare Biotechnology Co ltd
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Abstract

The utility model discloses a real-time fluorescence quantitative PCR appearance, including PCR appearance main part, the front end surface mosaic of PCR appearance main part has controls the panel, and the surface of controlling the panel is provided with temperature display screen and adjust the temperature knob, and the below of sampling detection box is provided with the water bath heating groove, and the water bath heating groove is located the inside of PCR appearance main part, install temperature sensor on the inside table wall of water bath heating groove, the bottom of water bath heating groove is provided with the electric heating groove, and the internally mounted of electric heating groove has electric heating pipe, through electric connection between temperature sensor and the temperature display screen, through electric connection between adjust the temperature knob and the electric heating pipe power control module. The utility model discloses a be provided with portable heating control mechanism, the convenience is sampled fixedly and is heated the detection thing, and this equipment possesses thermal-insulated heat dissipation and the good advantage of stability simultaneously.

Description

Real-time fluorescence quantitative PCR instrument
Technical Field
The utility model relates to a PCR appearance detects technical field, specifically is a real-time fluorescence ration PCR appearance.
Background
The PCR amplification apparatus, also known as PCR gene amplification apparatus, PCR nucleic acid amplification apparatus, is an apparatus for amplifying specific DNA by using PCR technology, and is widely used in medical and biological laboratories, for example, to determine whether a sample will show a map of a certain genetic disease, diagnose infectious diseases, copy genes, and identify parents. A PCR instrument with a fluorescence signal acquisition system and a computer analysis and processing system added on the basis of a common PCR instrument is called as a fluorescence quantitative PCR instrument. The PCR amplification principle is the same as that of common PCR instrument, and the primer added during PCR amplification is labeled with isotope, fluorescein, etc. and is used together with the specific template combination for amplification. The amplified result is transmitted to a computer analysis processing system through a fluorescent signal acquisition system for real-time signal acquisition and then is output as a quantized real-time result. This PCR instrument is called a real-time fluorescent quantitative PCR instrument. The fluorescence quantitative PCR instrument has single channel, double channels and multiple channels. When only one fluorescent probe is used for labeling, a single channel is selected, and when multiple fluorescent probes are used, multiple channels are used. The single channel can also detect the target gene expression product of multiple fluorescent markers, because the amplification amount of only one target gene can be detected at one time, and the amount of different target gene fragments can be detected only by multiple amplifications. The method is mainly used for medical clinical detection, biological medicine research and development, food industry, scientific research institutions and other institutions.
The current real-time fluorescence quantitative PCR instrument can not carry out convenient sampling and heating control on an object to be detected, so that the detection efficiency is low, and therefore, the market urgently needs to develop a novel real-time fluorescence quantitative PCR instrument.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a real-time fluorescence quantitative PCR appearance to current real-time fluorescence quantitative PCR appearance of proposing in solving above-mentioned background art can't treat the measuring thing and carry out portable sampling and heating control, leads to the lower problem of detection efficiency.
In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a real-time fluorescence ration PCR appearance, includes PCR appearance main part, the front end surface mosaic of PCR appearance main part has controls the panel, and the surface of controlling the panel is provided with temperature display screen and adjust the temperature knob, the upper surface of PCR appearance main part is provided with fixed recess, and the inside of fixed recess is provided with the sampling detection box, and the below of sampling detection box is provided with the water bath heating tank, and the water bath heating tank is located the inside of PCR appearance main part, install temperature sensor on the inside table wall of water bath heating tank, the bottom of water bath heating tank is provided with the electrical heating groove, and the internally mounted of electrical heating groove has electric heating pipe, through electric connection between temperature sensor and the temperature display screen, through electric connection between adjust the temperature knob and the electric heating pipe power control module, electric heating pipe and electric heating pipe power control module pass through electric connection.
Preferably, four corners of the bottom of the PCR instrument main body are respectively provided with a fixed sucker.
Preferably, the surfaces of the two sides of the PCR instrument main body are provided with heat dissipation grooves, a heat insulation plate is arranged in the PCR instrument main body, and the heat insulation plate is positioned below the electric heating groove.
Preferably, be movable detachable structure between sampling test box and the PCR appearance main part, the top of sampling test box is provided with sealed apron, and hinge and fixed recess both sides surface swing joint are passed through to the both ends of sealed apron.
Preferably, the surface of the sampling detection box is provided with a fixed slot, and a sampling test tube is arranged inside the fixed slot.
Preferably, the front end surface of the PCR instrument main body is provided with a power interface.
Compared with the prior art, the beneficial effects of the utility model are that:
(1) a water bath heating groove is arranged below the sampling detection box, a temperature sensor is arranged on the inner surface wall of the water bath heating groove, an electric heating groove is arranged at the bottom of the water bath heating groove, an electric heating pipe is arranged inside the electric heating groove, the sampling test tube is placed and fixed inside the sampling detection box, convenient heating detection is carried out on the sampling test tube by using water bath heating, the water temperature can be detected in real time by the temperature sensor, the water temperature value is displayed by a temperature display screen, and the power of the electric heating pipe can be controlled by using a temperature adjusting knob to be matched with a power control module of the electric heating pipe, so that the advantage of convenience in temperature control is achieved;
(2) the both sides surface of PCR appearance main part is provided with the radiating groove, and the radiating groove conveniently dispels the heat fast after heating internal heating mechanism, and the inside of PCR appearance main part is provided with the heat insulating board, and the heat insulating board can prevent effectively that inside heat from transmitting to table surface, and four corners in the bottom of PCR appearance main part all are provided with fixed sucking disc, and fixed sucking disc possesses the fixed effect of good actuation, stability when can increase PCR appearance main part operation.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention;
fig. 2 is a schematic view of the local internal structure of the present invention.
In the figure: 1. a PCR instrument main body; 2. a temperature display screen; 3. a control panel; 4. a temperature adjusting knob; 5. sealing the cover plate; 6. sampling a detection box; 7. fixing the slotted hole; 8. fixing the groove; 9. a power interface; 10. a heat sink; 11. sampling a test tube; 12. a water bath heating tank; 13. a temperature sensor; 14. an electrical heating tank; 15. an electric heating tube; 16. a heat insulation plate; 17. and fixing the sucker.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.
Referring to fig. 1-2, the present invention provides an embodiment: the utility model provides a real-time fluorescence quantitative PCR appearance, including PCR appearance main part 1, the front end surface mosaic of PCR appearance main part 1 has control panel 3, the surface of control panel 3 is provided with temperature display screen 2 and adjust the temperature knob 4, the upper surface of PCR appearance main part 1 is provided with fixed recess 8, the inside of fixed recess 8 is provided with sampling detection box 6, the below of sampling detection box 6 is provided with water bath heating tank 12, water bath heating tank 12 is located the inside of PCR appearance main part 1, install temperature sensor 13 on the inside table wall of water bath heating tank 12, the bottom of water bath heating tank 12 is provided with electrical heating tank 14, the internally mounted of electrical heating tank 14 has electric heating pipe 15, pass through electric connection between temperature sensor 13 and the temperature display screen 2, pass through electric connection between adjust the temperature knob 4 and the electric heating pipe power control module, electric heating pipe 15 passes through electric connection with electric heating pipe power control module.
Further, four corners of the bottom of the PCR instrument main body 1 are provided with fixed suckers 17.
Further, heat dissipation grooves 10 are formed in the surfaces of both sides of the PCR instrument body 1, and a heat insulation plate 16 is disposed inside the PCR instrument body 1, wherein the heat insulation plate 16 is located below the electric heating groove 14.
Further, be movable detachable structure between sampling test box 6 and PCR appearance main part 1, it is specific, sampling test box 6 can dismantle with PCR appearance main part 1 through the spiral shell and be connected, and sampling test box 6's top is provided with sealed apron 5, and hinge and 8 both sides surface swing joint of fixed recess are passed through at the both ends of sealed apron 5. Magnet is embedded in the bottom surface of sealing cover plate 5, and sealing cover plate 5 passes through the magnetic adsorption to be fixed in fixed recess 8, is convenient for accomodate.
Further, the surface of the sampling detection box 6 is provided with a fixed slot hole 7, and the inside of the fixed slot hole 7 is provided with a sampling test tube 11.
Further, the front end surface of the PCR instrument main body 1 is provided with a power interface 9.
The working principle is as follows: when in use, the upper surface of the PCR instrument main body 1 is provided with a fixed groove 8, the inside of the fixed groove 8 is provided with a sampling detection box 6, the surface of the sampling detection box 6 is provided with a fixed slotted hole 7, the inside of the fixed slotted hole 7 is provided with a sampling test tube 11, the sampling test tube 11 is placed and fixed inside the sampling detection box 6, the lower part of the sampling detection box 6 is provided with a water bath heating groove 12, the water bath heating groove 12 is positioned inside the PCR instrument main body 1, the inner surface wall of the water bath heating groove 12 is provided with a temperature sensor 13, the bottom of the water bath heating groove 12 is provided with an electric heating groove 14, the electric heating tube 15 is arranged inside the electric heating groove 14, the water bath heating is used for convenient heating detection, the water temperature can be detected in real time through the temperature sensor 13, the water temperature value is displayed through a temperature display screen 2, the power of the electric heating tube 15 can be controlled by the temperature adjusting knob 4 in cooperation with the electric heating tube power control module, thereby reach the convenient advantage of control by temperature change, the both sides surface of PCR appearance main part 1 is provided with radiating groove 10, and the inside of PCR appearance main part 1 is provided with heat insulating board 16, and radiating groove 10 conveniently dispels the heat fast after heating the internal heating mechanism, and heat insulating board 16 can prevent effectively that inside heat from transmitting to table surface, plays good thermal-insulated protection effect.
It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims (6)

1. The utility model provides a real-time fluorescence quantitative PCR appearance, includes PCR appearance main part (1), its characterized in that, the front end surface mosaic of PCR appearance main part (1) has control panel (3), and the surface of controlling panel (3) is provided with temperature display screen (2) and adjust temperature knob (4), the upper surface of PCR appearance main part (1) is provided with fixed recess (8), and the inside of fixed recess (8) is provided with sampling test box (6), and the below of sampling test box (6) is provided with water bath heating tank (12), and water bath heating tank (12) are located the inside of PCR appearance main part (1), install temperature sensor (13) on the inside table wall of water bath heating tank (12), and the bottom of water bath heating tank (12) is provided with electrical heating tank (14), and the internally mounted of electrical heating tank (14) has electric heating pipe (15), through electric connection between temperature sensor (13) and temperature display screen (2), the temperature adjusting knob (4) is electrically connected with the electric heating tube power control module, and the electric heating tube (15) is electrically connected with the electric heating tube power control module.
2. The real-time fluorescent quantitative PCR instrument according to claim 1, wherein: four corners of the bottom of the PCR instrument main body (1) are provided with fixed suckers (17).
3. The real-time fluorescent quantitative PCR instrument according to claim 1, wherein: the heat dissipation groove (10) is arranged on the surfaces of the two sides of the PCR instrument main body (1), the heat insulation plate (16) is arranged inside the PCR instrument main body (1), and the heat insulation plate (16) is located below the electric heating groove (14).
4. The real-time fluorescent quantitative PCR instrument according to claim 1, wherein: the sampling detection box (6) and the PCR instrument main body (1) are movably detachable, a sealing cover plate (5) is arranged above the sampling detection box (6), and two ends of the sealing cover plate (5) are movably connected with the surfaces of two sides of the fixed groove (8) through hinges.
5. The real-time fluorescent quantitative PCR instrument according to claim 1, wherein: the surface of the sampling detection box (6) is provided with a fixed slot hole (7), and a sampling test tube (11) is arranged inside the fixed slot hole (7).
6. The real-time fluorescent quantitative PCR instrument according to claim 1, wherein: the front end surface of the PCR instrument main body (1) is provided with a power interface (9).
CN202120847640.1U 2021-04-23 2021-04-23 Real-time fluorescence quantitative PCR instrument Active CN215250876U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202120847640.1U CN215250876U (en) 2021-04-23 2021-04-23 Real-time fluorescence quantitative PCR instrument

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202120847640.1U CN215250876U (en) 2021-04-23 2021-04-23 Real-time fluorescence quantitative PCR instrument

Publications (1)

Publication Number Publication Date
CN215250876U true CN215250876U (en) 2021-12-21

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202120847640.1U Active CN215250876U (en) 2021-04-23 2021-04-23 Real-time fluorescence quantitative PCR instrument

Country Status (1)

Country Link
CN (1) CN215250876U (en)

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