CN215667985U - Fluorescent quantitative PCR instrument - Google Patents
Fluorescent quantitative PCR instrument Download PDFInfo
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- CN215667985U CN215667985U CN202121745878.XU CN202121745878U CN215667985U CN 215667985 U CN215667985 U CN 215667985U CN 202121745878 U CN202121745878 U CN 202121745878U CN 215667985 U CN215667985 U CN 215667985U
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Abstract
The utility model discloses a fluorescent quantitative PCR instrument, which comprises a shell and a top cover, wherein the top cover is hinged with the shell through a hinge shaft, an EP pipe mounting piece is arranged in the shell, a plurality of mounting holes are formed in the EP pipe mounting piece, Peltier is arranged at the bottom in each mounting hole and used for accommodating an EP pipe to be tested, a plugging component is arranged on one side, facing the shell, of the top cover and can abut against the port of the EP pipe to be tested, and the plugging component is also used for heating the EP pipe to be tested. The fluorescent quantitative PCR instrument provided by the utility model can heat the upper end and the lower end of the EP tube to be detected through the plugging component and the Peltier simultaneously, so that the heating efficiency of the EP tube to be detected can be obviously improved, and the detection efficiency is improved.
Description
Technical Field
The utility model relates to the field of PCR detection, in particular to a fluorescent quantitative PCR instrument.
Background
Molecular detection diagnostic techniques based on gene detection are an important development field in the life of scientific health industry in recent years. The molecular detection mainly comprises three technologies of nucleic acid amplification, biochip and nucleic acid sequencing. The real-time fluorescent quantitative PCR nucleic acid amplification technology mainly based on the polymerase chain reaction has the advantages of high sensitivity, strong specificity, short window period and low cost, can be widely used for medical detection of infectious diseases, genetic diseases, tumors, personalized medicine, organ transplantation and the like, can also be used in the fields of import and export inspection and quarantine, food safety, judicial identification, animal (pet) medical treatment and the like, and is the highest point of development of the current molecular diagnosis technology. The equipment and reagents of the fluorescence quantitative PCR technology become the main products of the molecular diagnosis market at present.
Most of the existing PCR detection equipment adopts a technical route for alternately controlling high and low temperatures at fixed points, and has the advantages of simple structure and convenient control. However, the method has the main problems that long time is consumed in high-low temperature alternative control and cyclic detection, the heating speed is slow, one-time detection needs 3-4 hours, the detection efficiency is low, a user needs to wait for a long time, a detection result cannot be obtained in time, and great inconvenience is brought to the user.
SUMMERY OF THE UTILITY MODEL
The utility model mainly aims to provide a fluorescent quantitative PCR instrument, aiming at solving the technical problems.
In order to achieve the purpose, the fluorescent quantitative PCR instrument provided by the utility model comprises a shell and a top cover, wherein the top cover is hinged with the shell through a hinge shaft, an EP pipe mounting piece is arranged in the shell, a plurality of mounting holes are formed in the EP pipe mounting piece, Peltier is arranged at the bottom in each mounting hole and used for accommodating an EP pipe to be detected, a plugging component is arranged on one side, facing the shell, of the top cover and can abut against the port of the EP pipe to be detected, and the plugging component is also used for heating the EP pipe to be detected.
In one embodiment, the outer wall surface of the top cover is provided with a display and control screen.
In an embodiment, the shutoff subassembly is in including being annular mounting plate, setting a plurality of metal shutoff heads and the PCB board on the mounting plate, it is a plurality of the metal shutoff head is all installed on the PCB board, all there is the heater strip in the metal shutoff head, the heater strip with PCB board electric connection.
In an embodiment, the fluorescent quantitative PCR instrument further includes an adjusting component, and the adjusting component is disposed on the mounting base plate and connected to the PCB to adjust a distance between the metal plugging head and the EP tube mounting member.
In one embodiment, the adjusting assembly comprises a mounting plate which is U-shaped and is mounted on the mounting base plate, first connecting columns are arranged on two sides of the mounting plate, second connecting columns corresponding to the first connecting columns are arranged at two ends of the mounting base plate, and the first connecting columns are connected with the corresponding second connecting columns through elastic connecting pieces.
In an embodiment, the adjusting assembly further comprises an adjusting bolt, one end of the adjusting bolt is embedded in the top of the mounting plate, and the other end of the adjusting bolt penetrates through the top cover.
In an embodiment, the mounting panel includes roof and two curb plates, first spliced pole wears to establish the roof, seted up vertical spout on the curb plate, the tip correspondence of first spliced pole set up in the vertical spout.
In the technical scheme of the utility model, the fluorescent quantitative PCR instrument comprises a shell and a top cover, wherein the top cover is hinged with the shell through a hinged shaft, an EP pipe mounting part is arranged in the shell, a plurality of mounting holes are formed in the EP pipe mounting part, Peltier patches are arranged at the bottoms of the mounting holes, the mounting holes are used for accommodating an EP pipe to be tested, a plugging component is arranged on one side, facing the shell, of the top cover and can abut against the port of the EP pipe to be tested, the plugging component is also used for heating the EP pipe to be tested, in the technical scheme, after the EP pipe to be tested is arranged in the EP pipe mounting part, the top cover is covered on the shell, the plugging component can abut against the port of the EP pipe to be tested, and simultaneously the upper end and the lower end of the EP pipe to be tested can be heated through the plugging component and the Peltier patches, so that the heating efficiency of the EP pipe to be tested can be obviously improved, thereby improving the detection efficiency.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.
FIG. 1 is a schematic structural diagram of a fluorescence quantitative PCR instrument according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of the internal structure of the fluorescence quantitative PCR instrument according to the embodiment of the present invention;
fig. 3 is an enlarged schematic view of a point a in fig. 2.
The reference numbers illustrate: 10. a housing; 20. a top cover; 30. an EP tube mount; 40. a plugging component; 41. mounting a bottom plate; 42. a metal blocking head; 43. a PCB board; 44. a second connecting column; 50. an adjustment assembly; 51. mounting a plate; 511. a top plate; 512. a side plate; 513. a vertical chute; 52. a first connecting column; 53. an elastic connecting member; 54. adjusting the bolt; 60. and displaying a control screen.
The implementation, functional features and advantages of the objects of the present invention will be further explained with reference to the accompanying drawings.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that all the directional indicators (such as up, down, left, right, front, and rear … …) in the embodiment of the present invention are only used to explain the relative position relationship between the components, the movement situation, etc. in a specific posture (as shown in the drawing), and if the specific posture is changed, the directional indicator is changed accordingly.
In addition, the descriptions related to "first", "second", etc. in the present invention are only for descriptive purposes and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.
Moreover, the technical solutions in the embodiments of the present invention may be combined with each other, but it is necessary to be able to be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should be considered to be absent, and is not within the protection scope of the present invention.
The utility model provides a fluorescent quantitative PCR instrument.
As shown in fig. 1 and fig. 2, the fluorescence quantitative PCR instrument provided by the embodiment of the present invention includes a housing 10 and a top cover 20, where the top cover 20 is hinged to the housing 10 through a hinge shaft, an EP tube mounting member 30 is disposed in the housing 10, the EP tube mounting member 30 is provided with a plurality of mounting holes, the bottom inside the mounting holes is provided with peltier, the mounting holes are used for accommodating an EP tube to be measured, one side of the top cover 20 facing the housing 10 is provided with a plugging component 40, the plugging component 40 can abut against a port of the EP tube to be measured, and the plugging component 40 is further used for heating the EP tube to be measured.
In this embodiment, the fluorescence quantitative PCR instrument includes a housing 10 and a top cover 20, the top cover 20 is hinged to the housing 10 through a hinge shaft, an EP tube mounting member 30 is disposed in the housing 10, a plurality of mounting holes are disposed on the EP tube mounting member 30, peltier patches are disposed at the bottoms of the mounting holes, the mounting holes are used for accommodating an EP tube to be tested, a plugging component 40 is disposed on one side of the top cover 20 facing the housing 10, the plugging component 40 can abut against a port of the EP tube to be tested, the plugging component 40 is further used for heating the EP tube to be tested, in this technical scheme, after the EP tube to be tested is disposed in the EP tube mounting member 30, when the top cover 20 is covered on the housing 10, the plugging component 40 can abut against the port of the EP tube to be tested, and simultaneously the upper and lower ends of the EP tube to be tested can be heated through the plugging component 40 and the peltier patches, therefore, the heating efficiency of the EP pipe to be detected can be obviously improved, and the detection efficiency is improved.
It can be understood that, in this embodiment, the fluorescence quantitative PCR instrument further includes an optical detection module disposed in the housing 10, and the optical detection module is configured to scan fluorescence signals of the EP tubes to be detected, collect fluorescence signal data, and upload the fluorescence signal data to the upper computer.
Wherein, be provided with on the outer wall of top cap 20 and show screen control 60, after the EP pipe that awaits measuring is placed, at the in-process that shell 10 was established to top cap 20 lid, plugging component 40 initially can contact with the port of the EP pipe that awaits measuring earlier, later at further rotatory in-process, plugging component 40 can closely press fitting on the EP pipe that awaits measuring, thereby can make the EP pipe that awaits measuring can not rocked when detecting, promote stability, and when using in the field or portable carrying, can guarantee that the intraductal liquid of EP that awaits measuring can not reveal, further promote sealing performance, simultaneously after top cap 20 lid, show screen control 60 and can show the EP pipe fluorescence information that awaits measuring in real time, it shows through showing screen control after the fluorescence signal data is gathered again through the host computer, so that the staff looks over.
Specifically, shutoff subassembly 40 is in including being annular mounting plate 41, setting a plurality of metal shutoff heads 42 and PCB board 43 on the mounting plate 41, it is a plurality of metal shutoff head 42 all installs on the PCB board 43, all there is the heater strip in the metal shutoff head 42, the heater strip with PCB board 43 electric connection. In this embodiment, the PCB board 43 and the heating wire electric connection, therefore when the metal shutoff head 42 leans on the EP pipe that awaits measuring, can make the heating wire circular telegram heat, and the metal shutoff head 42 is because possessing good heat conductivility, can very fast with the heat conduction of heating wire to the EP pipe that awaits measuring intraductally, collocate the paler of bottom in the EP pipe installed part 30 simultaneously and paste to reach the purpose that the upper and lower both sides of the EP pipe that awaits measuring heaied up simultaneously, thereby promoted the heating efficiency of the EP pipe that awaits measuring.
Further, the fluorescent quantitative PCR instrument further comprises an adjusting assembly 50, wherein the adjusting assembly 50 is disposed on the mounting base plate 41 and connected to the PCB 43 to adjust a distance between the metal blocking head 42 and the EP tube mounting member 30. In this embodiment, since the EP tubes to be measured are not standardized, there may be a plurality of types of EP tubes to be measured having different heights, and the distance between the end of the metal blocking head 42 and the EP mounting member can be adjusted by the adjusting assembly 50, so as to adapt to the EP tubes to be measured having different heights, thereby improving the compatibility of the fluorescence quantitative PCR instrument.
Specifically, the adjusting assembly 50 includes and is the U type and installs mounting panel 51 on the mounting plate 41, the equal first spliced pole 52 in both sides of mounting panel 51, the both ends of mounting plate 41 be provided with the corresponding second spliced pole 44 of first spliced pole 52, first spliced pole 52 rather than corresponding connect through elastic connecting piece 53 between the second spliced pole 44. The adjusting assembly 50 further comprises an adjusting bolt 54, one end of the adjusting bolt 54 is embedded in the top of the mounting plate 51, and the other end of the adjusting bolt 54 penetrates through the top cover 20. In this embodiment, please refer to fig. 3, the mounting plate 51 includes a top plate 511 and two side plates 512, the first connecting column 52 penetrates through the top plate 511, a vertical sliding groove 513 is formed in the side plate 512, and an end of the first connecting column 52 is correspondingly disposed in the vertical sliding groove 513, so that in the process of rotation through the adjusting bolt 54, the adjusting bolt 54 can drive the top of the mounting plate 51 to move downward or upward, thereby changing the distance between the metal plug 42 and the EP tube to be measured, and meanwhile, the vertical sliding groove 513 can not only guide and slide the first connecting column 52, but also prevent the first connecting column 52 from being disconnected, so as to ensure stability.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention, and all modifications and equivalents of the present invention, which are made by the contents of the present specification and the accompanying drawings, or directly/indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (7)
1. The utility model provides a fluorescence quantitative PCR appearance, its characterized in that, fluorescence quantitative PCR appearance includes shell and top cap, the top cap pass through the articulated shaft with the shell is articulated to be connected, the inside EP pipe installed part that sets up of shell, be provided with a plurality of mounting holes on the EP pipe installed part, the inside bottom of mounting hole is provided with the Peltier, the mounting hole is used for the holding EP pipe that awaits measuring, the top cap faces one side of shell is provided with the shutoff subassembly, the shutoff subassembly can support and lean on the port department of the EP pipe that awaits measuring, the shutoff subassembly still is used for right the EP pipe that awaits measuring heats.
2. The fluorescence quantitative PCR instrument of claim 1, wherein the outer wall surface of the top cover is provided with a display screen.
3. The fluorescence quantitative PCR instrument of claim 2, wherein the blocking assembly comprises an annular mounting base plate, a plurality of metal blocking heads and a PCB, the metal blocking heads are arranged on the mounting base plate, the metal blocking heads are all mounted on the PCB, heating wires are all arranged in the metal blocking heads, and the heating wires are electrically connected with the PCB.
4. The fluorescence quantitative PCR instrument of claim 3, further comprising an adjusting component disposed on the mounting base plate and connected to the PCB plate to adjust a distance between the metal blocking head and the EP tube mounting member.
5. The fluorescence quantitative PCR instrument of claim 4, wherein the adjusting component comprises a U-shaped mounting plate mounted on the mounting base plate, the mounting plate has first connecting posts on both sides, the mounting base plate has second connecting posts corresponding to the first connecting posts at both ends, and the first connecting posts are connected with the corresponding second connecting posts through elastic connecting members.
6. The fluorescence quantitative PCR instrument of claim 5, wherein the adjusting assembly further comprises an adjusting bolt, one end of the adjusting bolt is embedded in the top of the mounting plate, and the other end of the adjusting bolt penetrates through the top cover.
7. The fluorescence quantitative PCR instrument of claim 6, wherein the mounting plate comprises a top plate and two side plates, the first connecting column penetrates through the top plate, a vertical sliding groove is formed in each side plate, and the end of the first connecting column is correspondingly arranged in the vertical sliding groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121745878.XU CN215667985U (en) | 2021-07-29 | 2021-07-29 | Fluorescent quantitative PCR instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121745878.XU CN215667985U (en) | 2021-07-29 | 2021-07-29 | Fluorescent quantitative PCR instrument |
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| Publication Number | Publication Date |
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| CN215667985U true CN215667985U (en) | 2022-01-28 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202121745878.XU Active CN215667985U (en) | 2021-07-29 | 2021-07-29 | Fluorescent quantitative PCR instrument |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117247832A (en) * | 2023-08-18 | 2023-12-19 | 湖南菲思特精准医疗科技有限公司 | Real-time fluorescence quantitative PCR analyzer and fluorescence detection method |
-
2021
- 2021-07-29 CN CN202121745878.XU patent/CN215667985U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117247832A (en) * | 2023-08-18 | 2023-12-19 | 湖南菲思特精准医疗科技有限公司 | Real-time fluorescence quantitative PCR analyzer and fluorescence detection method |
| CN117247832B (en) * | 2023-08-18 | 2024-04-12 | 湖南菲思特精准医疗科技有限公司 | Real-time fluorescence quantitative PCR analyzer and fluorescence detection method |
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