CN103060187B - Detecting device for detecting electrochemical genes - Google Patents
Detecting device for detecting electrochemical genes Download PDFInfo
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- CN103060187B CN103060187B CN201210590669.1A CN201210590669A CN103060187B CN 103060187 B CN103060187 B CN 103060187B CN 201210590669 A CN201210590669 A CN 201210590669A CN 103060187 B CN103060187 B CN 103060187B
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Abstract
The invention provides a detecting device for detecting electrochemical genes, which is connected with a gene chip. The detecting device comprises a loading module, a heating module, a probe module and a micro diaphragm gas pump, wherein the loading module is used for loading the gene chip; the heating module is used for heating the chip; the probe module is used for detecting the chip; the micro diaphragm gas pump is used for driving a fluid in the gene chip to move; the heating module and the probe module are arranged in the gene chip loading module and connected with the gene chip; the probe module comprises a probe fixing seat and a probe which is arranged on the probe fixing seat; at least one probe is arranged; the probe module outputs a detecting signal; and the micro diaphragm gas pump is connected with the heating module and an external power supply. Through adoption of an electrochemical detecting technology, the detecting device for detecting the electrochemical genes, provided by the invention, has the advantages of rapidness, stability, sensitivity, accuracy, wide detecting range and the like; and as the detecting device mainly uses an electric control technology and a computer technology, the detecting device provided by the invention has the advantages of no optical device, no moving part, small volume and simplicity and convenience in maintenance.
Description
Technical field
The present invention relates to technique of gene detection field, particularly relate to a kind of proofing unit detecting for electrochemical gene.
Background technology
Gene test is the technology by the DNA of blood, other body fluid or cell is detected.Gene test can diagnose the illness, also can be for the prediction of disease risks.Medical diagnosis on disease is to detect with technique of gene detection the mutator gene that causes heredopathia.Current most widely used gene test is virus detection, transmissible disease detection, the diagnosis of genetic diseases and the auxiliary diagnosis of some common disease.The feature of gene test is fast, accurately.The method of gene test at present mainly contains: quantitative fluorescent PCR, gene chip, liquid biochip and microflow control technique etc.
The most frequently used gene molecule measuring technology is quantitative fluorescent PCR (polymerase chain reaction), but this technology can only once be surveyed 1-2 kind gene.Need to a large amount of gene informations be carried out efficiently, detects fast, be analyzed, must utilize biochip technology.Gene chip plays a role in can not only diagnosing in early days; Compare with traditional detection method, it can, on a chip, carry out the detection of various diseases simultaneously to a plurality of patients; Utilize gene chip, can also understand disease from molecular level.These advantages of gene chip, can make medical worker grasp at short notice a large amount of medical diagnosis on disease information, find correct treatment measure.In addition, gene chip, at the screening of new drug, the aspects such as guidance of clinical application, also plays an important role.
At present, the gene chip of applying on market mainly contains three types: 1) be fixed on polymer matrix film (nylon membrane, nitrocellulose membranes etc.) lip-deep nucleic acid probe or cDNA fragment, be hybrid with it with isotope-labeled target gene conventionally, by radiography technology, detects.2) by point sample method, be fixed on the DNA probe array on sheet glass, by detecting with fluorescently-labeled target gene hybridization.3) direct synthetic oligonucleotide probe array on the hard surfaces such as glass, detects with fluorescently-labeled target gene hybridization.
The order-checking principle of gene chip is sequencing by hybridization method, and sequence to be measured, by hybridizing with the nucleic acid probe of one group of known array, is then determined result according to the detection of hybridization signal.Therefore the detection of hybridization signal is the key component in biochip technology.Gene chip is because used marker is different, thereby corresponding detection method is also different.Most researchers is used fluorescent marker, also has some investigators to use biotin labeling, and the color reaction of associating antibiotin binding substances (as in conjunction with chemical luminous substrate enzyme, fluorescein etc.) detects.Conventional fluorescent marker detects the method for hybridization signal at present, mostly by laser, the scanning of fluorescent substance is analyzed, detected, such as fluorescent microscope or the Fibre Optical Sensor of laser scanning fluorescent microscope, laser scanning confocal microscope, employing CCD camera.But all there are some shortcomings in current detection method:
Current fluorescently-labeled Laser Scanning, testing process is all more complicated, need pass through, the mark of fluorescent substance, hybridization in conjunction with, clean that non-specific fluorescent substance, chip are dry, the exciting of incident light, veiling glare filters, differentiate, collect the steps such as fluorescent signal; In testing process, need to use different equipment, such as hybridization device, cleaning equipment, test set etc.; Test set utilizes optical principle to detect mostly, and light path is complicated, apparatus expensive, often need to safeguard and difficult in maintenance, take time and effort; In the performance of test set, the detection sensitivity of dissimilar equipment, resolving power differ larger, all exist and detect poor repeatability, analyze the general problems such as narrower of enclosing.
Summary of the invention
Goal of the invention of the present invention is to overcome the defect of prior art, and a kind of proofing unit detecting for electrochemical gene that can greatly improve detection efficiency is provided.
In order to realize above-mentioned goal of the invention, the invention provides:
A kind of proofing unit detecting for electrochemical gene, be used for connecting gene chip, comprise: for loading, connect the loading module of gene chip, the heating module that is used to chip heating, the probe module for detecting, the miniature diaphragm air pump for driving gene chip inner fluid to move, described heating module and probe module are arranged in gene chip loading module, and are connected with gene chip; Described probe module comprises: probe permanent seat, be arranged on probe and signal transmission interface on probe permanent seat, described probe comprises at least one, and described miniature diaphragm air pump, heating module are connected with external power source.Use the advantage of miniature diaphragm air pump: efficiently, the voltage of random adjusting air pump, can be efficiently, vibrational frequency and the amplitude of random change tympanum, thus the flow velocity of miniflow pipeline can be regulated fast, meet the requirement of different in flow rate.The advantage of well heater: efficiently, operating voltage and the electric current of random regulation heating chip, obtain very easily different experimental temperatures, and can temperature be transmitted in electrochemical gene detection chip rapidly by copper base.Adopt probe groups input and output signal, can in limited space, realize hyperchannel, large-scale signal transmission, and effectively avoid interchannel signal to disturb mutually.
Preferably, described gene chip loading module comprises: hold down assembly and locating module, described in hold down assembly for gene chip is held out against and is fixed on locating module, on described locating module, be provided with gene chip installation position.Hold down assembly and being used in conjunction with of locating module, can change fast electrochemical gene detection chip, solve to greatest extent the size difference of electrochemical gene detection chip, very effective assurance electrochemical gene detection chip contact and probe groups are combined closely, and significantly improve the accuracy of experimental result.
Preferably, described holding down assembly comprises successively the base that connects, is arranged on pressure strip handle on base, is arranged on compact heap and the compact heap pressing plate in base, surveyed, described compact heap pressing plate is connected with base, and described compact heap is connected for fixing gene chip and controlling gene chip and miniature diaphragm air pump.
Preferably, described in hold down assembly and also comprise: tracheae and be arranged on the gas fittings on compact heap, described miniature diaphragm air pump is connected with tracheae, described tracheae is connected with gas fittings, described gas fittings is connected with gene chip.
Preferably, be provided with pressure strip handle return spring on described compact heap pressing plate, on described pressure strip handle, be provided with nose bar, described nose bar is connected with pressure strip handle return spring group.
Preferably, described locating module comprises: positioning base, strongback, card bead and gene chip installation position, described gene chip installation position is arranged on positioning base inner side, described strongback is arranged on gene chip installation position side, and described card bead is evenly arranged on positioning base both sides for carrying out spacing to the gene chip inserting.
Preferably, described locating module also comprises and lifts piece, described in lift piece and be connected with pressure strip handle, described in lift the side that piece is arranged on gene chip installation position, and near gene chip installation position opening part, described in lift piece for the disconnection of controlling gene chip and probe module be connected.
Preferably, be provided with probe module installation position on described locating module, described probe module installation position is a through hole, is provided with the buckle for stationary probe module on described installation position.
Preferably, be provided with heating module installation position on described locating module, described heating module installation position is a through hole.
Preferably, described heating module comprises interconnective well heater base and well heater, and described well heater comprises copper base, euthermic chip, resettable fuse, resistance and power interface.
Compared with prior art, the invention has the advantages that:
The application adopts the advantages such as electrochemical measuring technique has fast, stable, sensitive, accurate, sensing range is wide, and testing equipment mainly utilizes electronic control technology and computer technology, and without optics, movement-less part, volume is little, safeguards simple and convenient.Electrochemical measuring technique can be combined well with technique of gene detection, has complementary advantages, and has improved efficiency, susceptibility, reliability and industrialization, the standardization level of gene test comprehensively, has expanded the range of application of gene test.In addition, the applicability of electrochemical measuring technique has very large extendability, coupling or integration well in many detection fields.
The electrochemical scanning that the application adopts is three electrode alternating-current voltammetries (ACV) of employing standard, at volt-ampere, sends out the alternating-current of superpose in scanning fixed frequency and amplitude.The feature of this scanning is to reduce to disturb, and improves the sensitivity of detection signal, can detect the Weak current occurring on electrode and change, thereby improve the detection sensitivity of target gene.In addition, the chemical reaction current signal on scanning detecting electrode surface, also analyzes these faint current signals by the method for harmonic signal analysis.Harmonic signal analysis has higher immunity from interference, and signal to noise ratio is higher.Simultaneously, because need to pressing close to surface of metal electrode very much, the ferrocene molecule of mark could obtain volt-ampere sweep voltage, produce redox reaction, there is the generation of electrical signal, therefore target dna and the signal probe that obtains probe height specific hybridization at large can not be detected, so the scanning of gene chip does not need to clean not combined target gene sequence and signal probe.This has simplified the flow process of detection and the design of plant and instrument greatly, improves detection efficiency.And most of traditional biochip technologies need automatic equipment or do manually multiple stage fluid treating processes.
accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
Fig. 2 is compact heap structural representation of the present invention.
Fig. 3 is the structural representation of probe module of the present invention.
Fig. 4 is the structural representation of locating module of the present invention.
Fig. 5 is the structural representation of heating module of the present invention.
Fig. 6 is the structural representation of well heater of the present invention.
Fig. 7 is the syndeton of middle probe module of the present invention and base.
Embodiment
In embodiment, please supplement the concrete mode of connection of each assembly, if in the part the application who comprises for each module, need to explain please describe out, being that prior art is known can describe.
As shown in Fig. 1～6, the application provides a kind of proofing unit detecting for electrochemical gene, be used for connecting gene chip, specifically comprise: for loading, connect the loading module of gene chip, the heating module 3 that is used to chip heating, the probe module 4 for detecting, the miniature diaphragm air pump 5 for driving gene chip inner fluid to move, described heating module 3 and probe module 4 are arranged in gene chip loading module, and are connected with gene chip; Described probe module 4 comprises: probe permanent seat 41, be arranged on probe 42 and signal transmission interface on probe permanent seat 41, described probe 42 comprises at least one, and described miniature diaphragm air pump 5, heating module 3 are connected with external power source.
Further, during use, gene chip device, on apparatus module, is provided with chip installation position in this loading module, is provided with heating module 3 and probe module 4 on this chip installation position.
Particularly, these heating module 3 devices, in loading module, are connected with gene chip, and for gene chip provides heating, this probe module 4 is arranged on loading module inside equally, is connected with gene chip.
Embodiment 2
Further, on the basis of above-described embodiment 1, gene chip loading module comprises: hold down assembly 1 and locating module 2, hold down assembly and 1 for gene chip is held out against, be fixed on locating module 2, on locating module 2, be provided with gene chip installation position, 1 coupling corresponding with locating module 2 that hold down assembly connects, and jointly forms semi-surrounding structure, loads gene chip.
Further, hold down assembly and 1 comprise successively the base 11 that connects, be arranged on pressure strip handle 12 on base 11, be arranged on compact heap 13 and the compact heap pressing plate 14 in base, surveyed, described compact heap pressing plate 14 is connected with base 11, and compact heap 13 is connected for fixedly gene chip and controlling gene chip and miniature diaphragm air pump 5.
Wherein, pressing handle 12 is relations of interlock with compact heap 13, as shown in Figure 1, pressing handle 12 on the right, spring on compact heap pressing plate 14 lifts compact heap 13, and compact heap 13 is separated with the gene chip inserting, and pulls the centre of pressing handle 12 to Fig. 1, compact heap 13 is stretched, with gene chip laminating, the pipe joint on compact heap 13 and gene chip are connected, and gene chip is under the effect of compact heap 13 simultaneously, toward probe module 4, near also laminating, gene chip and probe are connected.Pull 12 go back tos the right of pressing handle, gene chip and compact heap 13, probe separates, can extract gene chip out again.
Further, hold down assembly on 1 and also comprise: tracheae 132 and be arranged on the gas fittings 133 on compact heap 13, miniature diaphragm air pump 5 is connected with tracheae 132, and tracheae 132 is connected with gas fittings 133, and gas fittings 133 is connected with gene chip.
Further, on this compact heap 13, be provided with gasket 131, gasket 131 is after installing gene chip, and for compressing gene chip, gas fittings 133 is arranged on gasket 131 simultaneously, and tracheae 132 is connected with gas fittings 133 through gasket 131.
Further, on compact heap pressing plate 14, have through hole, the through hole of gasket 131 from compact heap pressing plate 14 by and be connected with gene chip.
Further, be provided with pressure strip handle return spring 141 on block pressur plate 14, be provided with nose bar 121 on pressure strip handle 12, nose bar 121 is connected with 141 groups of pressure strip handle return springs.Form the resetting structure of pressure strip handle 12.
Further, locating module 2 comprises: positioning base 21, strongback 22, card bead 23 and gene chip installation position, gene chip installation position front end is provided with opening, for gene chip, insert, gene chip installation position is arranged on positioning base 21 inner sides, strongback 22 is arranged on gene chip installation position side or rear end, and card bead 23 is evenly arranged on positioning base 21 both sides for carrying out spacing to the gene chip inserting.
Further, on locating module 2, be also provided with and lift piece 24, utilize spring to lift, describedly lift the side that piece 24 is arranged on gene chip installation position, and near gene chip installation position opening part, described 24 bulge-structures of piece that lift, for making gene chip separated with probe module 4 when gene chip inserts gene location chip installation position, thereby when pulling pressure strip handle 12 under gene chip with lift piece and decline simultaneously and make gene chip be connected with probe module 4, and then the disconnection that realizes controlling gene chip and probe module be connected.
Further, be provided with probe module installation position on locating module 2, probe module installation position is a through hole, is provided with the buckle 26 for stationary probe module on described installation position.
As shown in Figure 7, buckle 26 is minor structures on positioning base 21.
Further, on locating module 2, be provided with heating module installation position 27, on described heating module installation position 27, be provided with spacer spring 28.
This spacer spring 28, for lifting heating module 3, makes heating module 3 separated with positioning base 21.
As shown in Fig. 6-7, further, heating module 3 comprises interconnective well heater base 37 and well heater 38, between well heater base 37 and well heater 38, by web member 39, connects.
Further, well heater 38 comprises substrate 31, is respectively arranged with euthermic chip 23, resistance 35, power interface 34, resettable fuse 33 on substrate 31.
Further, be provided with through hole 331 on well heater base 37, described resettable fuse 33 is through through hole 331.
Claims (5)
1. the proofing unit detecting for electrochemical gene, be used for connecting gene chip, it is characterized in that, comprise: for loading, connect the loading module of gene chip, the heating module that is used to chip heating, the probe module for detecting, the miniature diaphragm air pump for driving gene chip inner fluid to move, described heating module and probe module are arranged in gene chip loading module, and are connected with gene chip; Described probe module comprises: probe permanent seat, be arranged on probe and signal transmission interface on probe permanent seat, described probe comprises at least one, and described miniature diaphragm air pump, heating module are connected with external power source;
Described gene chip loading module comprises: hold down assembly and locating module, described in hold down assembly for gene chip is held out against and is fixed on locating module, on described locating module, be provided with gene chip installation position;
Described locating module comprises: positioning base, strongback, card bead and gene chip installation position, described gene chip installation position is arranged on positioning base inner side, described strongback is arranged on gene chip installation position side, and described card bead is evenly arranged on positioning base both sides for carrying out spacing to the gene chip inserting;
Described holding down assembly comprises successively the base that connects, is arranged on pressure strip handle on base, is arranged on compact heap and the compact heap pressing plate in base, surveyed, described compact heap pressing plate is connected with base, and described compact heap is connected for fixing gene chip and controlling gene chip and miniature diaphragm air pump;
Described holding down assembly also comprises: tracheae and be arranged on the gas fittings on compact heap, and described miniature diaphragm air pump is connected with tracheae, and described tracheae is connected with gas fittings, and described gas fittings is connected with gene chip;
On described compact heap pressing plate, be provided with pressure strip handle return spring, on described pressure strip handle, be provided with nose bar, described nose bar is connected with pressure strip handle return spring group.
2. the proofing unit detecting for electrochemical gene according to claim 1, it is characterized in that, described locating module also comprises and lifts piece, the described piece that lifts is connected with pressure strip handle, describedly lift the side that piece is arranged on gene chip installation position, and near gene chip installation position opening part, described in lift piece for the disconnection of controlling gene chip and probe module be connected.
3. the proofing unit detecting for electrochemical gene according to claim 1, it is characterized in that, on described locating module, be provided with probe module installation position, described probe module installation position is a through hole, is provided with the buckle for stationary probe module on described installation position.
4. the proofing unit detecting for electrochemical gene according to claim 1, is characterized in that, on described locating module, be provided with heating module installation position, described heating module installation position is a through hole.
5. the proofing unit detecting for electrochemical gene according to claim 1, it is characterized in that, described heating module comprises interconnective well heater base and well heater, and described well heater comprises copper base, euthermic chip, resettable fuse, resistance and power interface.
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| CN201210590669.1A CN103060187B (en) | 2012-12-31 | 2012-12-31 | Detecting device for detecting electrochemical genes |
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| CN201210590669.1A CN103060187B (en) | 2012-12-31 | 2012-12-31 | Detecting device for detecting electrochemical genes |
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| CN103224878B (en) * | 2013-05-08 | 2014-07-16 | 中山大学达安基因股份有限公司 | Gene chip based detection apparatus |
| CN107955778B (en) * | 2017-11-19 | 2021-03-19 | 杭州安弼晟生物科技有限公司 | Novel molecular diagnostic device |
| CN109187475B (en) * | 2018-09-17 | 2021-06-04 | 赵勇 | Gene chip mounting device and gene detection equipment thereof |
| CN112452945B (en) * | 2020-08-28 | 2023-09-15 | 武汉光谷航天三江激光产业技术研究院有限公司 | Device and method for high-precision laser cleaning and cleaning quality on-line monitoring |
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| JP2004028694A (en) * | 2002-06-24 | 2004-01-29 | Canon Inc | Temperature control device for nucleic acid probe array substrate, and gene detection method using the same |
| KR100612879B1 (en) * | 2004-12-06 | 2006-08-14 | 삼성전자주식회사 | Hybridization device using the control of pump and valves in closed system |
| CN102199529A (en) * | 2011-03-22 | 2011-09-28 | 博奥生物有限公司 | Biochip hybridization system |
| CN102533544B (en) * | 2011-12-31 | 2013-07-10 | 中山大学达安基因股份有限公司 | Electrochemical gene detection system |
| CN102559488A (en) * | 2012-01-16 | 2012-07-11 | 福建医科大学 | Quantitative polymerase chain reaction (PCR) microfluidic chip integrated device for integrated electrochemical detection technology |
| CN203048944U (en) * | 2012-12-31 | 2013-07-10 | 中山大学达安基因股份有限公司 | Electrochemical gene detecting device |
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Address after: No.19 Xiangshan Road, Science City, high tech Zone, Guangzhou, Guangdong 510665 Patentee after: Guangzhou Da'an gene Co.,Ltd. Address before: 510665 No. 19 incense Hill Road, hi tech Industrial Development Zone, Guangdong, Guangzhou Patentee before: DA AN GENE CO., LTD. OF SUN YAT-SEN University |