CN103060187A - Detecting device for detecting electrochemical genes - Google Patents
Detecting device for detecting electrochemical genes Download PDFInfo
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- CN103060187A CN103060187A CN2012105906691A CN201210590669A CN103060187A CN 103060187 A CN103060187 A CN 103060187A CN 2012105906691 A CN2012105906691 A CN 2012105906691A CN 201210590669 A CN201210590669 A CN 201210590669A CN 103060187 A CN103060187 A CN 103060187A
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Abstract
The invention provides a detecting device for detecting electrochemical genes, which is connected with a gene chip. The detecting device comprises a loading module, a heating module, a probe module and a micro diaphragm gas pump, wherein the loading module is used for loading the gene chip; the heating module is used for heating the chip; the probe module is used for detecting the chip; the micro diaphragm gas pump is used for driving a fluid in the gene chip to move; the heating module and the probe module are arranged in the gene chip loading module and connected with the gene chip; the probe module comprises a probe fixing seat and a probe which is arranged on the probe fixing seat; at least one probe is arranged; the probe module outputs a detecting signal; and the micro diaphragm gas pump is connected with the heating module and an external power supply. Through adoption of an electrochemical detecting technology, the detecting device for detecting the electrochemical genes, provided by the invention, has the advantages of rapidness, stability, sensitivity, accuracy, wide detecting range and the like; and as the detecting device mainly uses an electric control technology and a computer technology, the detecting device provided by the invention has the advantages of no optical device, no moving part, small volume and simplicity and convenience in maintenance.
Description
Technical field
The present invention relates to the technique of gene detection field, particularly relate to a kind of proofing unit for the electrochemical gene detection.
Background technology
Gene test is the technology that detects by the DNA to blood, other body fluid or cell.Gene test can diagnose the illness, and also can be used for the prediction of disease risks.Medical diagnosis on disease is to detect the mutator gene that causes heredopathia with technique of gene detection.Present most widely used gene test is virus detection, transmissible disease detection, the diagnosis of genetic diseases and the auxiliary diagnosis of some common disease.The characteristics of gene test are fast, accurately.The method of at present gene test mainly contains: quantitative fluorescent PCR, gene chip, liquid biochip and microflow control technique etc.
The most frequently used gene molecule measuring technology is quantitative fluorescent PCR (polymerase chain reaction), but this technology can only once be surveyed 1-2 kind gene.Need to carry out efficiently, detect fast, analyze a large amount of gene informations, must utilize biochip technology.Gene chip plays a role in can not only diagnosing in early days; Compare with traditional detection method, it can on a chip, carry out the detection of various diseases simultaneously to a plurality of patients; Utilize gene chip, can also understand disease from molecular level.These advantages of gene chip can make the medical worker grasp at short notice a large amount of medical diagnosis on disease information, find correct treatment measure.In addition, gene chip also plays an important role at the screening of new drug, the aspects such as guidance of clinical application.
At present, the gene chip of using on the market mainly contains three types: 1) be fixed on polymer matrix film (nylon membrane, nitrocellulose membranes etc.) lip-deep nucleic acid probe or cDNA fragment are hybrid with it with isotope-labeled target gene usually, detect by the radiography technology.2) be fixed on dna probe array on the sheet glass with the point sample method, by detecting with fluorescently-labeled target gene hybridization.3) oligonucleotide probe array that directly synthesizes on the hard surfaces such as glass detects with fluorescently-labeled target gene hybridization.
The order-checking principle of gene chip is the sequencing by hybridization method, and namely then sequence to be measured determines the result according to the detection of hybridization signal by hybridizing with the nucleic acid probe of one group of known array.So the detection of hybridization signal is the key component in the biochip technology.Gene chip is because employed marker is different, thereby corresponding detection method is also different.Most researchers is used fluorescent marker, also has some investigators to use biotin labeling, and the color reaction of associating antibiotin binding substances (as in conjunction with chemical luminous substrate enzyme, fluorescein etc.) detects.Fluorescent marker commonly used detects the method for hybridization signal at present, mostly by laser the scanning of fluorescent substance is analyzed, detected, such as fluorescent microscope or the Fibre Optical Sensor of laser scanning fluorescent microscope, laser scanning confocal microscope, employing CCD camera.But all there are some shortcomings in present detection method:
Present fluorescently-labeled Laser Scanning, testing process is all complicated, need pass through, the mark of fluorescent substance, hybridization in conjunction with, clean that non-specific fluorescent substance, chip are dry, the exciting of incident light, veiling glare filters, differentiate, collect the steps such as fluorescent signal; Need in the testing process to use different equipment, such as hybridization device, cleaning equipment, test set etc.; Test set utilizes optical principle to detect mostly, and light path is complicated, apparatus expensive, often need to safeguard and difficult in maintenance, take time and effort; On the performance of test set, the detection sensitivity of dissimilar equipment, resolving power differ larger, all have the detection poor repeatability, analyze the general problems such as narrower of enclosing.
Summary of the invention
Goal of the invention of the present invention is to overcome the defective of prior art, and a kind of proofing unit that electrochemical gene detects that is used for that can greatly improve detection efficiency is provided.
In order to realize above-mentioned goal of the invention, the invention provides:
A kind of proofing unit for the electrochemical gene detection, be used for connecting gene chip, comprise: be used for to load connect the loading module of gene chip, the heating module that is used to the chip heating, the probe module that is used for detecting, be used for driving the miniature diaphragm air pump that the gene chip inner fluid moves, described heating module and probe module are arranged in the gene chip loading module, and are connected with gene chip; Described probe module comprises: the probe permanent seat, be arranged on probe and signal transmission interface on the probe permanent seat, described probe comprises at least one, and described miniature diaphragm air pump, heating module are connected with external power source.Use the advantage of miniature diaphragm air pump: efficiently, the voltage of random adjusting air pump, can be efficiently, vibrational frequency and the amplitude of random change tympanum, thus the flow velocity of miniflow pipeline can be regulated fast, satisfy the requirement of different in flow rate.The advantage of well heater: efficiently, operating voltage and the electric current of random regulation heating chip, obtain very easily different experimental temperatures, and can temperature be transmitted on the electrochemical gene detection chip rapidly by copper base.Adopt the probe groups input and output signal, can in limited space, realize hyperchannel, large-scale signal transmission, and effectively avoid interchannel signal to disturb mutually.
Preferably, described gene chip loading module comprises: hold down assembly and locating module, described holding down assembly is fixed on locating module for gene chip is held out against, and is provided with the gene chip installation position on the described locating module.Hold down assembly and being used of locating module, can change fast the electrochemical gene detection chip, solve to greatest extent the size difference of electrochemical gene detection chip, very effective assurance electrochemical gene detection chip contact and probe groups are combined closely, and significantly improve the accuracy of experimental result.
Preferably, described holding down assembly comprises successively the base that connects, is arranged on pressure strip handle on the base, is arranged on compact heap and compact heap pressing plate on surveying in the base, described compact heap pressing plate is connected with base, and described compact heap is used for fixedly being connected of gene chip and controlling gene chip and miniature diaphragm air pump.
Preferably, described holding down assembly also comprises: tracheae and the gas fittings that is arranged on the compact heap, and described miniature diaphragm air pump is connected with tracheae, and described tracheae is connected with gas fittings, and described gas fittings is connected with gene chip.
Preferably, be provided with pressure strip handle return spring on the described compact heap pressing plate, be provided with nose bar on the described pressure strip handle, described nose bar is connected with pressure strip handle return spring group.
Preferably, described locating module comprises: positioning base, strongback, card bead and gene chip installation position, described gene chip installation position is arranged on the positioning base inboard, described strongback is arranged on gene chip installation position side, and described card bead evenly is arranged on the positioning base both sides and is used for carrying out spacing to the gene chip that inserts.
Preferably, described locating module also comprises and lifts piece, and the described piece that lifts is connected with the pressure strip handle, describedly lifts the side that piece is arranged on the gene chip installation position, and near gene chip installation position opening part, describedly lift disconnection that piece is used for controlling gene chip and probe module and be connected.
Preferably, be provided with the probe module installation position on the described locating module, described probe module installation position is a through hole, is provided with the buckle for the stationary probe module on the described installation position.
Preferably, be provided with the heating module installation position on the described locating module, described heating module installation position is a through hole.
Preferably, described heating module comprises interconnective well heater base and well heater, and described well heater comprises copper base, euthermic chip, resettable fuse, resistance and power interface.
Compared with prior art, the invention has the advantages that:
The application adopt electrochemical measuring technique have fast, stablize, sensitive, accurately, the advantage such as sensing range is wide, testing equipment mainly utilizes electronic control technology and computer technology, without optics device, movement-less part, volume is little, safeguards simple and convenient.Electrochemical measuring technique can be combined well with technique of gene detection, has complementary advantages, and has improved efficient, susceptibility, reliability and industrialization, the standardization level of gene test comprehensively, has expanded the range of application of gene test.In addition, the applicability of electrochemical measuring technique has very large extendability, well coupling or integration in many detection fields.
The electrochemical scanning that the application adopts is three electrode alternating-current voltammetries (ACV) of employing standard, namely sends out the alternating-current of scanning stack fixed frequency and amplitude at volt-ampere.The characteristics of this scanning are reduce disturbances, improve the sensitivity of detection signal, can detect the Weak current that occurs on the electrode and change, thereby improve the detection sensitivity of target gene.In addition, the chemical reaction current signal on scanning detecting electrode surface is also analyzed these faint current signals with the method for harmonic signal analysis.The harmonic signal analysis has higher immunity from interference, and signal to noise ratio is higher.Simultaneously, because need to pressing close to surface of metal electrode very much, the ferrocene molecule of mark could obtain the volt-ampere sweep voltage, produce redox reaction, the generation of electrical signal is arranged, therefore target dna and the signal probe that obtains the probe height specific hybridization at large can not be detected, so the scanning of gene chip does not need to clean not combined target gene sequence and signal probe.This has simplified the flow process of detection and the design of plant and instrument greatly, improves detection efficiency.And most of traditional biochip technologies need automatic equipment or do manually the multiple stage fluid treating processes.
Description of drawings
Fig. 1 is structural representation of the present invention.
Fig. 2 is compact heap structural representation of the present invention.
Fig. 3 is the structural representation of probe module of the present invention.
Fig. 4 is the structural representation of locating module of the present invention.
Fig. 5 is the structural representation of heating module of the present invention.
Fig. 6 is the structural representation of well heater of the present invention.
Fig. 7 is the syndeton of middle probe module of the present invention and base.
Embodiment
Please replenish the concrete mode of connection of each assembly among the embodiment, if please describe out for what need among the part the application who comprises in each module to explain, being that prior art is known can describe.
Shown in Fig. 1～6, the application provides a kind of proofing unit for the electrochemical gene detection, be used for connecting gene chip, specifically comprise: be used for to load connect the loading module of gene chip, the heating module 3 that is used to the chip heating, the probe module 4 that is used for detecting, be used for driving the miniature diaphragm air pump 5 that the gene chip inner fluid moves, described heating module 3 and probe module 4 are arranged in the gene chip loading module, and are connected with gene chip; Described probe module 4 comprises: probe permanent seat 41, be arranged on probe 42 and signal transmission interface on the probe permanent seat 41, described probe 42 comprises at least one, and described miniature diaphragm air pump 5, heating module 3 are connected with external power source.
Further, the gene chip device is provided with the chip installation position during use in this loading module on apparatus module, is provided with heating module 3 and probe module 4 on this chip installation position.
Particularly, these heating module 3 devices are connected with gene chip in loading module, and for gene chip provides heating, this probe module 4 is arranged on loading module inside equally, is connected with gene chip.
Embodiment 2
Further, on the basis of above-described embodiment 1, the gene chip loading module comprises: hold down assembly 1 and locating module 2, hold down assembly and 1 be used for gene chip held out against and be fixed on locating module 2, be provided with the gene chip installation position on the locating module 2, the 1 corresponding couplings with locating module 2 that hold down assembly connect, and jointly consist of the semi-surrounding structure, load gene chip.
Further, hold down assembly and 1 comprise successively the base 11 that connects, be arranged on pressure strip handle 12 on the base 11, be arranged on compact heap 13 and compact heap pressing plate 14 on surveying in the base, described compact heap pressing plate 14 is connected with base 11, and compact heap 13 is used for fixedly being connected of gene chip and controlling gene chip and miniature diaphragm air pump 5.
Wherein, pressing handle 12 is relations of interlock with compact heap 13, as shown in Figure 1, pressing handle 12 on the right, spring on the compact heap pressing plate 14 lifts compact heap 13, and compact heap 13 is to separate with the gene chip that inserts, and pulls the centre of pressing handle 12 to Fig. 1, compact heap 13 is stretched, fit with gene chip, the pipe joint on the compact heap 13 and gene chip are connected, and gene chip is under the effect of compact heap 13 simultaneously, near also fitting, gene chip and probe are connected toward probe module 4.Pull 12 go back tos the right of pressing handle, gene chip and compact heap 13, probe separates can be extracted gene chip out again.
Further, holding down assembly also comprises on 1: tracheae 132 and be arranged on gas fittings 133 on the compact heap 13, and miniature diaphragm air pump 5 is connected with tracheae 132, and tracheae 132 is connected with gas fittings 133, and gas fittings 133 is connected with gene chip.
Further, be provided with gasket 131 on this compact heap 13, gasket 131 is used for compressing gene chip after gene chip is installed, and gas fittings 133 is arranged on the gasket 131 simultaneously, and tracheae 132 is connected with gas fittings 133 through gasket 131.
Further, have through hole on the compact heap pressing plate 14, the through hole of gasket 131 from the compact heap pressing plate 14 by and be connected with gene chip.
Further, be provided with pressure strip handle return spring 141 on the block pressur plate 14, be provided with nose bar 121 on the pressure strip handle 12, nose bar 121 is connected with 141 groups of pressure strip handle return springs.Consist of the resetting structure of pressure strip handle 12.
Further, locating module 2 comprises: positioning base 21, strongback 22, card bead 23 and gene chip installation position, gene chip installation position front end is provided with opening, being used for gene chip inserts, the gene chip installation position is arranged on positioning base 21 inboards, strongback 22 is arranged on gene chip installation position side or rear end, and card bead 23 evenly is arranged on positioning base 21 both sides and is used for carrying out spacing to the gene chip that inserts.
Further, also be provided with on the locating module 2 and lift piece 24, utilize spring to lift, describedly lift the side that piece 24 is arranged on the gene chip installation position, and near gene chip installation position opening part, described 24 bulge-structures of piece that lift, be used for when gene chip inserts gene location chip installation position, gene chip being separated with probe module 4, thereby when pulling pressure strip handle 12 under the gene chip be connected piece and descend simultaneously gene chip is connected with probe module 4, and then the disconnection that realizes controlling gene chip and probe module be connected.
Further, be provided with the probe module installation position on the locating module 2, the probe module installation position is a through hole, is provided with the buckle 26 for the stationary probe module on the described installation position.
As shown in Figure 7, buckle 26 is minor structures on the positioning base 21.
Further, be provided with heating module installation position 27 on the locating module 2, be provided with spacer spring 28 on the described heating module installation position 27.
This spacer spring 28 is used for lifting heating module 3, and heating module 3 is separated with positioning base 21.
Shown in Fig. 6-7, further, heating module 3 comprises interconnective well heater base 37 and well heater 38, connects by web member 39 between well heater base 37 and the well heater 38.
Further, well heater 38 comprises substrate 31, is respectively arranged with euthermic chip 23, resistance 35, power interface 34, resettable fuse 33 at substrate 31.
Further, be provided with through hole 331 on the well heater base 37, described resettable fuse 33 passes through hole 331.
Claims (10)
1. one kind is used for the proofing unit that electrochemical gene detects, be used for connecting gene chip, it is characterized in that, comprise: be used for to load connect the loading module of gene chip, the heating module that is used to the chip heating, the probe module that is used for detecting, be used for driving the miniature diaphragm air pump that the gene chip inner fluid moves, described heating module and probe module are arranged in the gene chip loading module, and are connected with gene chip; Described probe module comprises: the probe permanent seat, be arranged on probe and signal transmission interface on the probe permanent seat, described probe comprises at least one, and described miniature diaphragm air pump, heating module are connected with external power source.
2. the proofing unit that detects for electrochemical gene according to claim 1, it is characterized in that, described gene chip loading module comprises: hold down assembly and locating module, described holding down assembly is fixed on locating module for gene chip is held out against, and is provided with the gene chip installation position on the described locating module.
3. the proofing unit that detects for electrochemical gene according to claim 2, it is characterized in that, described holding down assembly comprises successively the base that connects, is arranged on pressure strip handle on the base, is arranged on compact heap and compact heap pressing plate on surveying in the base, described compact heap pressing plate is connected with base, and described compact heap is used for fixedly being connected of gene chip and controlling gene chip and miniature diaphragm air pump.
4. the proofing unit that detects for electrochemical gene according to claim 3, it is characterized in that, described holding down assembly also comprises: tracheae and the gas fittings that is arranged on the compact heap, described miniature diaphragm air pump is connected with tracheae, described tracheae is connected with gas fittings, and described gas fittings is connected with gene chip.
5. the proofing unit that detects for electrochemical gene according to claim 3, it is characterized in that, be provided with pressure strip handle return spring on the described compact heap pressing plate, be provided with nose bar on the described pressure strip handle, described nose bar is connected with pressure strip handle return spring group.
6. the proofing unit that detects for electrochemical gene according to claim 2, it is characterized in that, described locating module comprises: positioning base, strongback, card bead and gene chip installation position, described gene chip installation position is arranged on the positioning base inboard, described strongback is arranged on gene chip installation position side, and described card bead evenly is arranged on the positioning base both sides and is used for carrying out spacing to the gene chip that inserts.
7. the proofing unit that detects for electrochemical gene according to claim 6, it is characterized in that, described locating module also comprises and lifts piece, the described piece that lifts is connected with the pressure strip handle, describedly lift the side that piece is arranged on the gene chip installation position, and near gene chip installation position opening part, describedly lift disconnection that piece is used for controlling gene chip and probe module and be connected.
8. the proofing unit that detects for electrochemical gene according to claim 5, it is characterized in that, be provided with the probe module installation position on the described locating module, described probe module installation position is a through hole, is provided with the buckle for the stationary probe module on the described installation position.
9. the proofing unit for the electrochemical gene detection according to claim 4 is characterized in that be provided with the heating module installation position on the described locating module, described heating module installation position is a through hole.
10. the proofing unit that detects for electrochemical gene according to claim 1, it is characterized in that, described heating module comprises interconnective well heater base and well heater, and described well heater comprises copper base, euthermic chip, resettable fuse, resistance and power interface.
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| CN201210590669.1A CN103060187B (en) | 2012-12-31 | 2012-12-31 | Detecting device for detecting electrochemical genes |
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| CN201210590669.1A CN103060187B (en) | 2012-12-31 | 2012-12-31 | Detecting device for detecting electrochemical genes |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103224878A (en) * | 2013-05-08 | 2013-07-31 | 中山大学达安基因股份有限公司 | Gene chip based detection apparatus |
| CN107955778A (en) * | 2017-11-19 | 2018-04-24 | 杭州安弼晟生物科技有限公司 | Novel molecular diagnostic device |
| CN109187475A (en) * | 2018-09-17 | 2019-01-11 | 赵勇 | A kind of detection device and its gene detection equipment |
| CN112452945A (en) * | 2020-08-28 | 2021-03-09 | 武汉光谷航天三江激光产业技术研究院有限公司 | High-precision laser cleaning and cleaning quality on-line monitoring device and method |
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Address after: No.19 Xiangshan Road, Science City, high tech Zone, Guangzhou, Guangdong 510665 Patentee after: Guangzhou Da'an gene Co.,Ltd. Address before: 510665 No. 19 incense Hill Road, hi tech Industrial Development Zone, Guangdong, Guangzhou Patentee before: DA AN GENE CO., LTD. OF SUN YAT-SEN University |
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