CN103224878B - Gene chip based detection apparatus - Google Patents
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- CN103224878B CN103224878B CN201310166553.XA CN201310166553A CN103224878B CN 103224878 B CN103224878 B CN 103224878B CN 201310166553 A CN201310166553 A CN 201310166553A CN 103224878 B CN103224878 B CN 103224878B
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Abstract
The invention relates to the technical field of gene detection, and concretely relates to a gene chip based detection apparatus. The apparatus comprises a loading module, a chip heating module and a probe module, the chip heating module and the probe module are arranged on the loading module, the probe module is provided with a probe group, the apparatus also comprises an electrochemical gene chip installed on the loading module and a circuit board used for providing an electrochemical scan excitation voltage for the electrochemical gene chip and detecting the signal current generated in the detection process of the electrochemical gene chip, and the chip heating module is in close contact with the electrochemical gene chip; and the circuit board is arranged outside the loading module, and is in electric connection with the electrochemical gene chip through the probe group. The apparatus adopts an electrochemical detection technology to carry out gene detection without adopting an optical device or complex running parts, has a simple structure and is convenient to maintain.
Description
Technical field
The present invention relates to technique of gene detection field, more specifically, relate to a kind of proofing unit based on gene chip.
Background technology
Gene test is the technology by the DNA of blood, other body fluid or cell is detected.Gene test can diagnose the illness, also can be for the prediction of disease risks.Medical diagnosis on disease is to detect with technique of gene detection the mutator gene that causes heredopathia.Current most widely used gene test is virus detection, transmissible disease detection, the diagnosis of genetic diseases and the auxiliary diagnosis of some common disease.The feature of gene test is fast, accurately.The method of gene test at present mainly contains: quantitative fluorescent PCR, gene chip (gene sensor), liquid biochip and micro-fluidic chip technology etc.
The most frequently used gene molecule measuring technology is quantitative fluorescent PCR (polymerase chain reaction), but this technology can only once be surveyed 1-2 kind gene.Need to a large amount of gene informations be carried out efficiently, detects fast, be analyzed, must utilize biochip technology.Gene chip plays a role in can not only diagnosing in early days; Compared with traditional detection method, it can, on a chip, carry out the detection of various diseases simultaneously to multiple patients; Utilize gene chip, can also understand disease from molecular level.These advantages of gene chip, can make medical worker grasp at short notice a large amount of medical diagnosis on disease information, find correct treatment measure.In addition, gene chip, at the aspect such as screening, the guidance of clinical application of new drug, also plays an important role.
The order-checking principle of gene chip is sequencing by hybridization method, and sequence to be measured, by hybridizing with the nucleic acid probe of one group of known array, is then determined result according to the detection of hybridization signal.Therefore the detection of hybridization signal is the key component in biochip technology.Gene chip is due to used marker difference, thereby corresponding detection method is also different.Most researchers is used fluorescent marker, also has some investigators to use biotin labeling, and the color reaction of associating antibiotin binding substances (as in conjunction with chemical luminous substrate enzyme, fluorescein etc.) detects.Conventional fluorescent marker detects the method for hybridization signal at present, mostly by laser, the scanning of fluorescent substance is analyzed, detected, such as fluorescent microscope or the Fibre Optical Sensor of laser scanning fluorescent microscope, laser scanning confocal microscope, employing CCD camera.But all there are some shortcomings in current detection method:
(1) fluorescently-labeled Laser Scanning, testing process is all more complicated, need by the mark of fluorescent substance, hybridization in conjunction with, clean that non-specific fluorescent substance, chip are dry, the exciting of incident light, veiling glare filters, differentiate, collect the steps such as fluorescent signal;
(2) in testing process, need to use different equipment, such as hybridization device, cleaning equipment, test set etc.;
(3) test set utilizes optical principle to detect mostly, light path complexity, apparatus expensive, often need to safeguard and difficult in maintenance, take time and effort;
(4) in the performance of test set, the detection sensitivity of dissimilar equipment, resolving power differ larger, all exist and detect poor repeatability, analyze the general problems such as narrower of enclosing.
Summary of the invention
The present invention is at least one defect (deficiency) overcoming described in above-mentioned prior art, and a kind of proofing unit based on gene chip simple in structure is provided.
For solving the problems of the technologies described above, technical scheme of the present invention is as follows:
A kind of proofing unit based on gene chip, comprise loading module, chip heating module and probe module, chip heating module and probe module are arranged on loading module, on probe module, be provided with probe groups, the circuit card that also comprises the electrochemical gene chip that is arranged on loading module and electrochemical gene chip and detect for the signalling current that provides Electrochemical Scanning energizing voltage to electrochemical gene chip and electrochemical gene chip is produced in testing process, chip heating module and electrochemical gene chip close contact; Circuit card is arranged on the outside of loading module, and it is electrically connected with electrochemical gene chip by probe groups.
As a kind of preferred version, described electrochemical gene chip is provided with fluid cavity, channel module, the probe circuit plate fitting with channel module, channel module is provided with micro-flow groove, channel module is contacted and is formed miniflow pipeline by micro-flow groove and probe circuit plate surface with after the laminating of probe circuit plate, fluid cavity is connected with miniflow pipeline and forms circulation loop, on probe circuit plate, be provided with reference electrode along miniflow pipeline, supporting electrode and the working electrode that is fixed with unimolecular layer and DNA capture probe, reference electrode, supporting electrode is connected with circuit card by probe groups respectively with working electrode.
As further preferred version, reference electrode, supporting electrode and working electrode array are arranged, wherein reference electrode comprises one, reference electrode is positioned at miniflow pipeline foremost, working electrode comprises several, supporting electrode comprises interconnective two, and two supporting electrodes are arranged on the two ends of all working electrode.
As preferred version further, on described circuit card, be provided with analog to digital conversion circuit, this analog to digital conversion circuit is connected with reference electrode by probe groups, and analog to digital conversion circuit gathers the absolute volt of reference electrode as the reference voltage of Electrochemical Scanning energizing voltage.
As preferred version further, on described circuit card, be provided with Electrochemical Scanning pump circuit and the working electrode array scanning and the switching circuit that are connected with Electrochemical Scanning pump circuit, described Electrochemical Scanning pump circuit is connected with supporting electrode and working electrode by probe groups, a supporting electrode forms multiple electrode pairs with several working electrodes respectively, and working electrode array scanning and switching circuit control volt-ampere scanning and excitation circuit carry out Electrochemical Scanning to each electrode pair in turn.
As preferred version further, on described circuit card, be provided with current detection circuit and the data processing circuit being connected with current detection circuit; Current detection circuit is connected with each working electrode by probe groups, for the signalling current of each electrode of Polling work; The data analysis processing of data processing circuit for current detection circuit is detected.
As a kind of preferred version, on described circuit card, be provided with probe groups interface, chip heating module interface and external interface, described probe groups interface is connected with probe groups, and chip heating module interface is connected with chip heating module, and external interface is used for connecting peripheral equipment.
As a kind of preferred version, also comprise that mutual coupling is connected to form drain pan and the upper casing of semi-surrounding structure, circuit card and loading module are all arranged in semi-surrounding structure, and wherein circuit card is arranged in drain pan, and loading module is arranged on circuit card top.
As a kind of preferred version, it is characterized in that, on described loading module, be provided with chip installation position, electrochemical gene chip is arranged on chip installation position with removably.
Compared with prior art, the beneficial effect of technical solution of the present invention is:
(1) the present invention adopts electrochemical measuring technique to carry out gene test, without adopting optics and complicated operation parts, simple in structure, easy to maintenance.
(2) the present invention adopts electrochemical measuring technique to scan and detects electrochemical gene chip power stream signal, the current signal collecting by analysis just can draw the result of gene order to be detected, and it is quick, sensitive, stable and sensing range is wide that electrochemical measuring technique has advantages of.
(3) the present invention carries out thermal conduction by chip heating module is set to electrochemical gene chip, chip heating module can fast, at random regulate operating voltage and the electric current of himself, thereby can regulate the temperature of electrochemical gene chip in testing process, for gene test provides various experimental temperatures.
(4) the present invention also utilizes probe groups to carry out input and output signal, can in the finite space of loading module, realize hyperchannel, large-scale signal transmission, and can effectively avoid interchannel signal to disturb.
Brief description of the drawings
Fig. 1 is the structural representation of a kind of proofing unit specific embodiment 1 based on gene chip in the present invention.
Fig. 2 is the structural representation of electrochemical gene chip in the present invention.
Fig. 3 is the structural representation of electrochemical gene chip in the present invention.
Fig. 4 is the structural representation of circuit card in the present invention.
Fig. 5 is the structural representation holding down assembly of loading module in the present invention.
Fig. 6 is the structural representation of the locating module of loading module in the present invention.
Fig. 7 is the phantom view of Fig. 6.
Fig. 8 is the structural representation of chips heating module of the present invention.
Fig. 9 is the structural representation of well heater in the present invention.
Figure 10 is the structural representation of middle probe module of the present invention.
Embodiment
Accompanying drawing, only for exemplary illustration, can not be interpreted as the restriction to this patent;
For better explanation the present embodiment, some parts of accompanying drawing have omission, zoom in or out, and do not represent the size of actual product;
To those skilled in the art, in accompanying drawing some known features and explanation thereof may to omit be understandable.
Below in conjunction with drawings and Examples, technical scheme of the present invention is described further.
Embodiment 1
As shown in Figure 1, a kind of proofing unit based on gene chip, comprise loading module 1, chip heating module 2, probe module 3, electrochemical gene chip 4 and circuit card 5, chip heating module 2, probe module 3 and electrochemical gene chip 4 are arranged on loading module 1, on probe module 3, be provided with probe groups, chip heating module 2 and electrochemical gene chip 4 close contacts, circuit card 5 is arranged on the outside of loading module 1, it is electrically connected with electrochemical gene chip 4 by probe groups, circuit card 5 detects for the signalling current of Electrochemical Scanning energizing voltage being provided to electrochemical gene chip 4 and to produce in testing process electrochemical gene chip 4.
Based on such scheme, the present invention applies Electrochemical Scanning energizing voltage by circuit card 5 to electrochemical gene chip 4 and gathers the signalling current that electrochemical gene chip 4 produces in testing process and carries out analyzing and processing, the signalling current collecting by analysis just can draw the result of gene order to be detected, and it is quick, sensitive, stable and sensing range is wide that electrochemical measuring technique has advantages of.And the present invention adopts electrochemical measuring technique to carry out gene test, without adopting optics and complicated operation parts, testing process is simple, easy to maintenance.In addition, the present invention carries out thermal conduction by chip heating module 2 is set to electrochemical gene chip 4, chip heating module 2 can fast, at random regulate operating voltage and the electric current of himself, thereby can regulate the temperature of electrochemical gene chip 4 in testing process, for gene test provides various experimental temperatures.The present invention also utilizes probe groups to carry out input and output signal, can in the finite space of loading module 1, realize hyperchannel, large-scale signal transmission, and can effectively avoid interchannel signal to disturb.
One preferred embodiment in, as shown in Figure 1, the present embodiment also comprises that mutual coupling is connected to form drain pan 6 and the upper casing 7 of semi-surrounding structure, circuit card 5 and loading module 1 are all arranged in semi-surrounding structure, wherein circuit card 5 is arranged in drain pan 6, and loading module 1 is arranged on circuit card 5 tops.
One preferred embodiment in, as shown in Figure 1, on loading module 1, be provided with chip installation position 13, electrochemical gene chip 4 is arranged on chip installation position 13 with removably.Electrochemical gene chip 4 is arranged in loading module 1 with removably, can insert easily and extract.Because electrochemical gene chip 4 is consumptive materials of gene test, in loading module 1, inserting an electrochemical gene chip 4 completes after test, this electrochemical gene chip 4 can be dismantled to the new electrochemical gene chip 4 of rear insertion just can test, simple and convenient, reproducible, without cleaning, safeguard simple and convenient.
One preferred embodiment in, the electrochemical gene chip of the present embodiment adopts following structure to realize:
As shown in Figures 2 and 3, electrochemical gene chip is provided with fluid cavity 41, channel module 42, the probe circuit plate 43 fitting with channel module 42, channel module 42 is provided with micro-flow groove 421, channel module 42 is contacted and is formed miniflow pipeline 44 by micro-flow groove 421 and probe circuit plate 43 surfaces with after 43 laminatings of probe circuit plate, fluid cavity 42 is connected with miniflow pipeline 44 and forms circulation loop, on probe circuit plate 43, be provided with reference electrode 431 along miniflow pipeline 44, supporting electrode 432 and the working electrode 433 that is fixed with unimolecular layer and DNA capture probe, reference electrode 431, supporting electrode 432 is connected with circuit card by probe groups respectively with working electrode 433.In this embodiment, in working electrode 433, be fixed with the capture probe of unimolecular layer and particular sequence, fluid cavity 41 is interior can load hybridization solution, and the present invention utilizes homologous sequence (pathogenic agent target dna) in capture probe and hybridization solution and the specific recognition effect of the signal probe taking electroactive substance as mark to carry out gene test.The sequence of signal probe and capture probe is designed to and the complementation of corresponding pathogenic agent DNA sequence dna, has electroactive substance ferrocene molecule to serve as a mark on signal probe; Hybridization solution flows out and enters into miniflow pipeline 44 from fluid cavity 41, hybridization solution contacts with working electrode 433, signal probe, forms after complicated specific hybrid between capture probe and pathogenic agent DNA, and the ferrocene molecule on signal probe is close to working electrode 433 surfaces; When having while applying voltage by circuit card to the working electrode 433 of electrochemical gene chip, ferrocene molecule can produce redox reaction, causes the generation of electric current, gene order to be measured just can be detected by circuit card to the analysis of current signal.Because electrochemical gene chip internal forms circulation loop, hybridization solution is recycled in fluid cavity 41 from the interior outflow of fluid cavity 41 after miniflow pipeline, reclaim simple and convenient, only need from proofing unit, dismantle electrochemical gene chip can reclaim hybridization solution, clear without whole proofing unit is carried out, safeguard simple and convenient.
Preferably, as shown in Figure 2, reference electrode 431, supporting electrode 432 and working electrode 433 along miniflow pipeline 44 with array mode spread configuration;
Reference electrode 431 comprises one, is positioned at miniflow pipeline 44 foremost, for the scanning of electrochemistry volt-ampere provides absolute reference voltage;
Working electrode 433 can arrange different numbers as required, but be subject to size and the miniflow pipeline 44 liquid circulation effect limits of probe circuit plate 43, magnitude setting can be between 2-128, each working electrode 433 can be fixed unimolecular layer and a kind of specific DNA capture probe, specific target dna is detected, therefore, each working electrode 433 is independently, and each working electrode 433 can interconnect by the circuit on probe circuit plate 43 and circuit card;
Supporting electrode 432 comprises two, be positioned at the two ends of all working electrode 433, and two supporting electrodes 432 interconnect, it interconnects and can realize by the circuit on probe circuit plate 43, after two supporting electrodes 432 are directly communicated with, can be interconnected with circuit card by the circuit on probe circuit plate 43 again.Two supporting electrodes 432 form some to electrode pair with each working electrode 433 respectively, when circuit card carries out the scanning of electrochemistry volt-ampere, each electrode pair wheel scan flow is detected, and apply a volt-ampere scanning and excitation voltage between supporting electrode 432 and working electrode 433, and detect the current signal in loop simultaneously.
In the present invention, electrochemical gene chip can adopt above-mentioned concrete structure to realize, but it does not limit said structure, and it is being as the criterion with the gene test that cooperatively interacted of circuit card, probe module.
In specific implementation process, described circuit card can adopt following structure to realize:
On circuit card, be provided with analog to digital conversion circuit, Electrochemical Scanning pump circuit, working electrode array scanning and switching circuit, current detection circuit and data processing circuit;
Analog to digital conversion circuit is connected with reference electrode by probe groups, and analog to digital conversion circuit gathers the absolute volt of reference electrode as the reference voltage of Electrochemical Scanning energizing voltage, to produce energizing voltage accurately.
Electrochemical Scanning pump circuit is connected with supporting electrode and working electrode by probe groups, working electrode array scanning is connected with Electrochemical Scanning pump circuit with switching circuit, a supporting electrode forms multiple electrode pairs with several working electrodes respectively, and working electrode array scanning and switching circuit control volt-ampere scanning and excitation circuit carry out Electrochemical Scanning to each electrode pair in turn.
Current detection circuit is connected with each working electrode by probe groups, and data processing circuit is connected with current detection circuit, the signalling current of each electrode of current detection circuit Polling work; The data analysis processing of data processing circuit for current detection circuit is detected.Wherein, data processing circuit can adopt the realization such as arm processor, dsp processor.
In specific implementation process, as shown in Figure 4, circuit card 5 is arranged on drain pan 6, on circuit card 5, be provided with probe groups interface 51, chip heating module interface 52 and external interface 53, probe groups interface 51 is connected with probe groups, chip heating module interface 52 is connected with chip heating module, and external interface 53 is for connecting peripheral equipment.Meanwhile, on circuit card 5, external power interface can also be set, this external power source structure can be connected external power source with chip heating module by power circuit board 5.
Embodiment 2
On the basis of embodiment 1, in order to regulate the flow velocity of hybridization solution in electrochemical gene chip, as shown in Figure 1, the present embodiment also comprises miniature diaphragm air pump 8, for electrochemical gene chip provides positive and negative air-pressure, it is connected with external power source, and is connected with electrochemical gene chip 4, moves for the fluid that drives electrochemical gene chip 4.
Loading module in the present embodiment can adopt following structure to realize:
As shown in Figure 1, loading module 1 comprises and holds down assembly 11 and locating module 12,11 couplings corresponding with locating module 12 that hold down assembly connect, common formation semi-surrounding structure, chip installation position 13 is arranged on locating module 12 and is positioned at semi-surrounding structure, electrochemical gene chip 4 is arranged in chip installation position 13, and 11 electrochemical gene chip 4 is held out against and is fixed on locating module 12 by holding down assembly.
Further, as shown in Figure 5, hold down assembly and 11 comprise successively the base 111 that connects, be arranged on the pressure strip handle 112 on base 111, the compact heap 113 that is arranged on base 111 inner sides and compact heap pressing plate 114, compact heap pressing plate 114 is connected with base 111, and compact heap 113 is for fixing electrochemical gene chip and control being connected of electrochemical gene chip and miniature diaphragm air pump.
Wherein, pressure strip handle 112 is relations of interlock with compact heap 113, as shown in Figure 1, pressure strip handle 112 is in the time of rightmost, compact heap can be in bottom, slowly pressure strip handle 112 is moved toward centre, spring on compact heap 113 lifts compact heap, the electrochemical gene chip 4 of compact heap and insertion is to separate, pull pressure strip handle 112 to middle, compact heap is stretched, fit with electrochemical gene chip 4, gas fittings on compact heap and electrochemical gene chip 4 are connected, simultaneous electrochemical gene chip 4 is under the effect of compact heap, close and the laminating toward probe module 3, electrochemical gene chip 4 is connected with probe groups.Pull again 112 go back tos the right of pressure strip handle, electrochemical gene chip 4 and compact heap, Probe components from, can extract electrochemical gene chip 4 out.
Further, as shown in Figure 5, on this compact heap 113, be provided with tracheae 1131 and gasket 1132, gasket 1132 is being installed after electrochemical gene chip, be used for compressing electrochemical gene chip, be provided with gas fittings 1133 on gasket 1132, miniature diaphragm air pump is connected with tracheae 1131 simultaneously, tracheae 1131 is connected with gas fittings 1133 through gasket 1132, and gas fittings 1133 is connected with electrochemical gene chip again.
Further, as shown in Figure 5, on compact heap pressing plate 114, have through hole, the through hole of gasket 1132 from compact heap pressing plate 114 passes through and is connected with electrochemical gene chip.
Further, as shown in Figure 5, be provided with pressure strip handle return spring 1141 on compact heap pressing plate 114, on pressure strip handle 112, be provided with nose bar, nose bar is connected with 1141 groups of pressure strip handle return springs.Form the resetting structure of pressure strip handle 112.
Further, as shown in Figure 6, locating module 12 comprises: positioning base 121, strongback 122, card bead 123, chip installation position 13 is arranged on locating module 12 and front end is provided with opening, insert for electrochemical gene chip, chip installation position 13 is arranged on positioning base 121 inner sides, and strongback 122 is arranged on chip installation position 13 sides or rear end, and card bead 123 is evenly arranged on positioning base 121 both sides for carrying out spacing to the electrochemical gene chip inserting.It is spacing that card bead 123 and strongback 122 are all that the electrochemical gene chip to inserting carries out, and electrochemical gene chip inserts to be encountered after strongback 122, and electrochemical gene chip can not continue toward interpolation again, prevents from inserting excessively.After putting in place, card bead 123 can be blocked electrochemical gene chip, prevents that electrochemical gene chip from running out.
Further, on locating module 12, be also provided with and lift piece 124, utilize spring to lift, lift the side that piece 124 is arranged on chip installation position 13, and near chip installation position 13 opening parts, lifting piece 124 is a bulge-structure, for electrochemical gene chip is separated in the time that electrochemical gene chip inserts chip installation position 13 with probe module, thereby in the time pulling pressure strip handle under the effect of compact heap electrochemical gene chip with lift piece 124 and decline and make electrochemical gene chip be connected with probe module simultaneously, and then realize and control the disconnection of electrochemical gene chip and probe module and be connected.The effect of lifting piece 124 is mainly to prevent from inserting electrochemical gene chip to encounter probe groups; encountering probe groups can touch probe groups curved; electrochemical gene chip also is likely inserted not enter; add and lifted after piece 124; while inserting electrochemical gene chip; electrochemical gene chip can be lifted certain altitude, can not encounter probe groups, plays the effect of protection probe groups.Lift piece 124 by spring supporting, when compact heap compresses electrochemical gene chip while moving pressure strip handle, lift piece 124 and be depressed simultaneously, electrochemical gene chip just can link together with probe groups.
Further, as shown in Figure 7, be provided with probe module installation position on locating module 12, probe module installation position is through-hole structure, is provided with the buckle 126 for stationary probe module on probe module installation position, and buckle 126 is minor structures on positioning base.
Further, be provided with chip heating module installation position on locating module, chip heating module is provided with spacer spring on installation position.This spacer spring is used for lifting chip heating module, and chip heating module is separated with positioning base.
As Figure 8-9, further, chip heating module comprises interconnective well heater base 21 and well heater 22, between well heater base 21 and well heater 22, connects by web member 23.
Further, well heater 22 comprises substrate 221, is respectively arranged with euthermic chip 222, resistance 223, power interface 224, resettable fuse 225 on substrate 221.Wherein, substrate 221 is as thermally conductive material, for the heat of euthermic chip 222 is passed to electrochemical gene chip.On substrate 221, design heating power device, temperature sensor and pilot circuit, realize power device to substrate 221 direct heating, and realize Temperature Feedback and temperature control by temperature sensor and pilot circuit.Chip heating module and electrochemical gene chip close contact, and electrochemical gene chip is accurately heated, for electrochemical gene chip hybridization and the scanning of electrochemistry volt-ampere provide essential temperature.
In the present embodiment, probe module can adopt following structure to realize:
As shown in figure 10, probe module 3 comprises: probe permanent seat 31, be arranged on probe groups 32 and signal transmission interface on probe permanent seat 31, at least comprise a probe in probe groups 32.
The corresponding same or analogous parts of same or analogous label;
In accompanying drawing, describe position relationship for only for exemplary illustration, can not be interpreted as the restriction to this patent;
Obviously, the above embodiment of the present invention is only for example of the present invention is clearly described, and is not the restriction to embodiments of the present invention.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here without also giving exhaustive to all embodiments.All any amendments of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in the protection domain of the claims in the present invention.
Claims (6)
1. the proofing unit based on gene chip, comprise loading module, chip heating module and probe module, chip heating module and probe module are arranged on loading module, on probe module, be provided with probe groups, it is characterized in that, also comprise the electrochemical gene chip being arranged on loading module and the circuit card detecting for the signalling current that provides Electrochemical Scanning energizing voltage to electrochemical gene chip and electrochemical gene chip is produced in testing process, chip heating module and electrochemical gene chip close contact; Circuit card is arranged on the outside of loading module, and it is electrically connected with electrochemical gene chip by probe groups;
The probe circuit plate that described electrochemical gene chip is provided with fluid cavity, channel module, fits with channel module, channel module is provided with micro-flow groove, channel module is contacted and is formed miniflow pipeline by micro-flow groove and probe circuit plate surface with after the laminating of probe circuit plate, fluid cavity is connected with miniflow pipeline and forms circulation loop, on probe circuit plate, be provided with reference electrode, supporting electrode and be fixed with unimolecular layer and the working electrode of DNA capture probe along miniflow pipeline, reference electrode, supporting electrode and working electrode are connected with circuit card by probe groups respectively;
Described reference electrode, supporting electrode and working electrode array are arranged, wherein reference electrode comprises one, and reference electrode is positioned at miniflow pipeline foremost, and working electrode comprises several, supporting electrode comprises interconnective two, and two supporting electrodes are arranged on the two ends of all working electrode;
On described circuit card, be provided with analog to digital conversion circuit, this analog to digital conversion circuit is connected with reference electrode by probe groups, and analog to digital conversion circuit gathers the absolute volt of reference electrode as the reference voltage of Electrochemical Scanning energizing voltage;
Each working electrode is independently.
2. the proofing unit based on gene chip according to claim 1, it is characterized in that, on described circuit card, be provided with Electrochemical Scanning pump circuit and the working electrode array scanning and the switching circuit that are connected with Electrochemical Scanning pump circuit, described Electrochemical Scanning pump circuit is connected with supporting electrode and working electrode by probe groups, a supporting electrode forms multiple electrode pairs with several working electrodes respectively, and working electrode array scanning and switching circuit control Electrochemical Scanning pump circuit carry out Electrochemical Scanning to each electrode pair in turn.
3. the proofing unit based on gene chip according to claim 1, is characterized in that, is provided with current detection circuit and the data processing circuit being connected with current detection circuit on described circuit card; Current detection circuit is connected with each working electrode by probe groups, for the signalling current of each electrode of Polling work; The data analysis processing of data processing circuit for current detection circuit is detected.
4. the proofing unit based on gene chip according to claim 1, it is characterized in that, on described circuit card, be provided with probe groups interface, chip heating module interface and external interface, described probe groups interface is connected with probe groups, chip heating module interface is connected with chip heating module, and external interface is used for connecting peripheral equipment.
5. the proofing unit based on gene chip according to claim 1, it is characterized in that, also comprise that mutual coupling is connected to form drain pan and the upper casing of semi-surrounding structure, circuit card and loading module are all arranged in semi-surrounding structure, wherein circuit card is arranged in drain pan, and loading module is arranged on circuit card top.
6. according to the proofing unit based on gene chip described in claim 1 to 5 any one, it is characterized in that, on described loading module, be provided with chip installation position, electrochemical gene chip is arranged on chip installation position with removably.
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| CN107807232B (en) * | 2016-09-09 | 2020-05-26 | 京元电子股份有限公司 | Test module of biochip and test equipment thereof |
| CN109187475B (en) * | 2018-09-17 | 2021-06-04 | 赵勇 | Gene chip mounting device and gene detection equipment thereof |
| CN111239026B (en) * | 2018-11-29 | 2022-08-05 | 肖光文 | Immune cell movement speed detection system |
| CN110031531B (en) * | 2019-04-28 | 2021-12-14 | 河海大学常州校区 | Device for connecting electrochemical workstation and microelectrode array and operation method thereof |
| CN110982666A (en) * | 2019-12-19 | 2020-04-10 | 深圳市华迈生物医疗科技有限公司 | Device, system and method for real-time fluorescence quantitative nucleic acid amplification detection |
| CN112592818A (en) * | 2020-12-18 | 2021-04-02 | 覃日华 | Automatic detection equipment for genotyping chip |
| CN114210377B (en) * | 2021-12-21 | 2023-01-06 | 哈尔滨工业大学 | Portable multifunctional visual microfluid equipment based on electric field regulation and control |
| CN114437911B (en) * | 2022-01-13 | 2023-08-01 | 深圳清华大学研究院 | Gene sequencing device |
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| CN100401048C (en) * | 2005-05-19 | 2008-07-09 | 复旦大学 | Multiple channel micro-flow control chip, process for making same and use thereof |
| KR100725374B1 (en) * | 2006-05-02 | 2007-06-07 | 삼성전자주식회사 | Dna chip, dna chip kit and method for fabricating dna chip |
| US20080087554A1 (en) * | 2006-05-24 | 2008-04-17 | Antara Biosciences Inc. | Electrochemical detection device |
| CN103060187B (en) * | 2012-12-31 | 2014-04-23 | 中山大学达安基因股份有限公司 | Detecting device for detecting electrochemical genes |
| CN203269943U (en) * | 2013-05-08 | 2013-11-06 | 中山大学达安基因股份有限公司 | Detection device based on gene chip |
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