CN219348718U - Reagent detection device and electronic equipment - Google Patents
Reagent detection device and electronic equipment Download PDFInfo
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- CN219348718U CN219348718U CN202223506432.9U CN202223506432U CN219348718U CN 219348718 U CN219348718 U CN 219348718U CN 202223506432 U CN202223506432 U CN 202223506432U CN 219348718 U CN219348718 U CN 219348718U
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The application belongs to the technical field of detection, provides a reagent detection device and electronic equipment, and reagent detection device includes: sample base plate, working electrode, biological probe, sensing electrode, power interface, working electrode interface, sensing electrode interface and field effect transistor. In this application, sample base plate, working electrode, sensing electrode and biological probe are connected with field effect transistor through the externally-hung mode, when needs carry out sample solution and examine time, only need drop sample solution and add on sample base plate, working electrode, sensing electrode and biological probe, after the test is accomplished, can remove sample base plate, working electrode, sensing electrode and biological probe through the mode with working electrode, sensing electrode and the separation of working electrode interface and sensing electrode interface, field effect transistor can recycle, every time test need to change new sample base plate, working electrode, sensing electrode and biological probe, greatly reduced the cost of test.
Description
Technical Field
The application belongs to the technical field of detection, and particularly relates to a reagent detection device and electronic equipment.
Background
The reagent detection device is a sensor for quantitatively analyzing the matrix content in the sample liquid by utilizing the molecular recognition ability of biological materials such as microorganisms, enzymes, antibodies, DNA, RNA and the like and using the biological materials as molecular recognition elements. That is, the substrate contained in the sample liquid is quantitatively analyzed by utilizing a reaction generated when the biological material is recognized as the target substrate, for example, by utilizing oxygen consumption, enzyme reaction, luminescence, or the like caused by respiration of the microorganism.
The existing reagent detection device generally directly modifies the biological probe on the gate of the field effect tube, so that the biological probe and the field effect tube are used as consumable materials at the same time, and the detection cost is greatly caused.
Disclosure of Invention
In order to solve the technical problems, the embodiment of the application provides a reagent detection device and electronic equipment, and aims to solve the problem that the existing reagent detection device is high in detection cost.
A first aspect of embodiments of the present application provides a reagent detection device, the reagent detection device comprising:
a sample substrate for receiving a sample solution;
the working electrode is arranged on the sample substrate;
a biological probe arranged on the working electrode;
the induction electrode is arranged on the sample substrate, and the induction electrode and the working electrode are not contacted with each other;
the power interface is used for accessing pulse voltage;
a working electrode interface;
the induction electrode interface and the working electrode interface are used for respectively connecting the induction electrode and the working electrode;
and the drain electrode of the field effect transistor is connected with the power interface, and the grid electrode of the field effect transistor is connected with the induction electrode interface.
In one embodiment, the reagent detection device further comprises:
and the testing circuit is connected with the source electrode of the field effect transistor and is used for detecting the current output by the source electrode of the field effect transistor to generate a current detection signal and generating a detection signal of the object to be detected according to the current detection signal.
In one embodiment, the distance between the working electrode and the sense electrode is equal to the distance between the working electrode interface and the sense electrode interface.
In one embodiment, the working electrode interface and the sensing electrode interface are female receptacle interfaces, and the female receptacle interfaces mate with the working electrode and the sensing electrode.
In one embodiment, the working electrode and the sensing electrode are affixed to the sample substrate.
In one embodiment, the sample substrate is a glass substrate or a plastic substrate.
In one embodiment, the sensing electrode and the working electrode are disposed in parallel.
In one embodiment, the distance between the sensing electrode and the working electrode is less than 1 μm.
In one embodiment, the material of the induction electrode and the working electrode is any one of gold, silver, copper, iron and aluminum.
A second aspect of embodiments of the present application provides an electronic device, including: the reagent detecting apparatus according to any one of the above.
Compared with the prior art, the embodiment of the application has the beneficial effects that: the sample substrate, the working electrode, the sensing electrode and the biological probe are connected with the field effect transistor in an externally hung mode, when the sample solution is required to be detected, the sample solution is only required to be dripped on the sample substrate, the working electrode, the sensing electrode and the biological probe, after the detection is finished, the sample substrate, the working electrode, the sensing electrode and the biological probe can be removed in a mode of separating the working electrode, the sensing electrode from a working electrode interface and a sensing electrode interface, and the field effect transistor is not contacted with the sample solution, so that the field effect transistor can be recycled, and the sample substrate, the working electrode, the sensing electrode and the biological probe are required to be replaced each time of the detection, thereby greatly reducing the cost of the detection.
Drawings
FIG. 1 is a schematic diagram of a reagent detecting apparatus according to one embodiment of the present application;
FIG. 2 is a schematic diagram II of a reagent detecting apparatus according to an embodiment of the present application;
fig. 3 is a schematic diagram of a reagent detection device according to an embodiment of the present disclosure.
Detailed Description
In order to make the technical problems, technical schemes and beneficial effects to be solved by the present application more clear, the present application is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the present application.
It will be understood that when an element is referred to as being "mounted" or "disposed" on another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element.
It is to be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the present application and simplify description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be configured and operated in a particular orientation, and therefore should not be construed as limiting the present application.
Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present application, the meaning of "a plurality" is one or more than one unless specifically defined otherwise.
Reference in the specification to "one embodiment," "some embodiments," or "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in one or more embodiments of the application. Thus, appearances of the phrases "in one embodiment," "in some embodiments," "in other embodiments," "in a specific embodiment," "in a specific application," or the like in various places throughout this specification are not necessarily all referring to the same embodiment, but mean "one or more but not all embodiments" unless specifically emphasized otherwise. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.
The reagent detection device is a sensor for quantitatively analyzing the matrix content in the sample liquid by utilizing the molecular recognition ability of biological materials such as microorganisms, enzymes, antibodies, DNA, RNA and the like and using the biological materials as molecular recognition elements. That is, the substrate contained in the sample liquid is quantitatively analyzed by utilizing a reaction generated when the biological material is recognized as the target substrate, for example, by utilizing oxygen consumption, enzyme reaction, luminescence, or the like caused by respiration of the microorganism.
The existing reagent detection device generally directly modifies the biological probe on the gate of the field effect tube, so that the biological probe and the field effect tube are used as consumable materials at the same time, and the detection cost is greatly caused.
To solve the above technical problem, an embodiment of the present application provides a reagent detection device, referring to fig. 1 and 2, the reagent detection device includes: sample substrate 10, working electrode 20, bio-probe 30, sensing electrode 40, power interface 50, working electrode interface 60, sensing electrode interface 70, and field effect transistor 80.
Specifically, the sample substrate 10 is used for receiving a sample solution. The working electrode 20 is provided on the sample substrate 10. The biological probe 30 is provided on the working electrode 20. The sensing electrode 40 is disposed on the sample substrate 10, and the sensing electrode 40 and the working electrode 20 are not in contact with each other. The power interface 50 is used to switch on the pulsed voltage. The working electrode interface 60 is connected to the power interface 50. Sense electrode interface 70 and working electrode interface 60 are used to connect sense electrode 40 and working electrode 20, respectively. The drain 81 of the field effect transistor 80 is connected to the power interface 50, and the gate 82 of the field effect transistor 80 is connected to the sense electrode interface 70.
In this embodiment, when it is required to test whether the sample solution contains the object to be detected, the sample solution can be dripped onto the sample substrate 10, because the working electrode 20, the sensing electrode 40 and the biological probe 30 are all disposed above the sample substrate 10, and the working electrode 20, the sensing electrode 40 and the biological probe 30 need to be covered by the sample solution.
In this embodiment, it can be understood that the sample substrate 10 and the working electrode 20 and the sensing electrode 40 on the sample substrate 10 are connected to the field effect transistor 80 through the working electrode interface 60 and the sensing electrode interface 70 by plugging. Specifically, when a sample solution is to be tested, working electrode 20 is connected to drain 81 of field effect transistor 80 via working electrode interface 60, and sensing electrode 40 is connected to gate 82 of field effect transistor 80 via sensing electrode interface 70. The sample solution is dripped on the working electrode 20, the sensing electrode 40 and the biological probe 30, and the power interface 50 is connected with pulse voltage, at this time, whether the sample solution contains an object to be detected can be determined by detecting the current magnitude in the channel of the field effect transistor 80.
In this embodiment, the sample substrate 10, the working electrode 20, the sensing electrode 40 and the biological probe 30 are connected with the field effect transistor 80 by means of plug-in connection, when the sample solution is required to be detected, the sample solution is only required to be dripped on the sample substrate 10, the working electrode 20, the sensing electrode 40 and the biological probe 30, after the test is completed, the sample substrate 10, the working electrode 20, the sensing electrode 40 and the biological probe 30 can be removed by means of separating the working electrode 20, the sensing electrode 40 from the working electrode interface 60 and the sensing electrode interface 70, and since the field effect transistor 80 is not contacted with the sample solution, the field effect transistor 80 can be recycled, and the sample substrate 10, the working electrode 20, the sensing electrode 40 and the biological probe 30 need to be replaced each time, thereby greatly reducing the test cost.
In one embodiment, the bioprobe 30 is: antibodies/antigens/nucleic acids, and the like.
In one embodiment, the bioprobe 30 is: a DNA fragment or an RNA fragment.
In one embodiment, referring to fig. 3, the reagent detecting apparatus further includes: the circuit 90 is tested.
Specifically, the test circuit 90 is connected to the source 83 of the field effect transistor 80, and the test circuit 90 is configured to detect a current output from the source 83 of the field effect transistor 80 to generate a current detection signal, and generate a detection signal of the object to be detected according to the current detection signal.
In the present embodiment, the source 83 of the field effect transistor 80 is grounded, and the circuit under test is connected to the source 83 of the field effect transistor 80. When the solution contains the object to be detected, the object to be detected in the detection solution will react with the biological probe 30 in a combined manner, an induced potential will be generated between the working electrode 20 and the sensing electrode 40, the sensing electrode 40 is connected to the gate 82 of the field effect transistor 80 through the sensing electrode interface 70, so that the induced potentials generated by the gate 82 of the field effect transistor 80 are different, and the test circuit 90 can detect the current output by the source 83 of the field effect transistor 80 to generate a current detection signal, and generate the object to be detected detection signal according to the current detection signal. For example, when the voltage of the current detection signal is greater than the preset voltage, a first level detection signal of the to-be-detected object is generated to indicate that the sample solution contains the to-be-detected object at the moment, when the voltage of the current detection signal is less than or equal to the preset voltage, a second level detection signal of the to-be-detected object is generated to indicate that the sample solution does not contain the to-be-detected object at the moment,
in this embodiment, by detecting the current of the source 83 of the field effect transistor 80, whether the sample solution contains the object to be detected is determined according to the current, and a long detection time is required to be waited, in the experiment, the detection time is only 1 minute, so that the detection time is greatly shortened, and in addition, the sensitivity of detecting the sample solution by using the field effect transistor 80 can be improved to 1pg/mL, so that the detection sensitivity is greatly improved.
In one embodiment, the distance between working electrode 20 and sense electrode 40 is equal to the distance between working electrode interface 60 and sense electrode interface 70.
In the present embodiment, the distance between the working electrode 20 and the sensing electrode 40 refers to the distance from the center of the working electrode 20 to the center of the sensing electrode 40. The distance between the working electrode interface 60 and the sensing electrode interface 70 refers to the distance from the center of the working electrode interface 60 to the center of the sensing electrode interface 70, and in this embodiment, by setting the distance between the working electrode 20 and the sensing electrode 40 to be equal to the distance between the working electrode interface 60 and the sensing electrode interface 70, the working electrode 20 and the sensing electrode 40 can be accurately inserted when being inserted into the working electrode interface 60 and the sensing electrode interface 70, so that an insertion error is avoided, and the stability of the reagent detection device is improved.
In one embodiment, working electrode interface 60 and sense electrode interface 70 are female receptacle interfaces, and the female receptacle interfaces mate with working electrode 20 and sense electrode 40. For example, the working electrode interface 60 and the sensing electrode interface 70 are female socket interfaces, the working electrode 20 and the sensing electrode 40 are male socket plugs, and the female socket interfaces and the male socket plugs are matched, so that the working electrode 20 and the sensing electrode 40 on the sample substrate 10 can be accurately connected with the field effect transistor 80 through the working electrode interface 60 and the sensing electrode interface 70, the plugging error is avoided, and the stability of the reagent detection device is improved.
In one embodiment, working electrode 20 and sensing electrode 40 are affixed to sample substrate 10.
Specifically, the working electrode 20 and the sensing electrode 40 are adhered to the sample substrate 10 through the insulating colloid, so that the working electrode 20 and the sensing electrode 40 can be fixed, the problem that the working electrode 20 and the sensing electrode 40 move in the working process to influence the final detection result is avoided, and the stability of the reagent detection device is improved.
In one embodiment, the material used for the sample substrate 10 is an insulating material.
In the present embodiment, by using the insulating material for the sample substrate 10, the influence of the working electrode 20 and the sensing electrode 40 on the sample substrate 10 during the operation is avoided, and the error is reduced.
In one embodiment, the sample substrate 10 is a glass substrate or a plastic substrate.
Specifically, the glass substrate or the plastic substrate is a good insulating material, and the cost is low, so that the influence of the sample substrate 10 on the working electrode 20 and the sensing electrode 40 in the working state can be reduced by arranging the sample substrate 10 as the glass substrate or the plastic substrate, the error is reduced, and the cost can be reduced.
In one embodiment, sense electrode 40 and working electrode 20 are disposed in parallel.
Specifically, the bio-probe 30 is disposed on the working electrode 20, when the sample solution contains the object to be detected, the bio-probe is combined with the bio-probe 30, and induced electromotive force is generated between the working electrode 20 and the sensing electrode 40, and by setting the sensing electrode 40 and the working electrode 20 in parallel, the strength of the generated induced electromotive force is stronger, the detection accuracy is increased, and the detection accuracy is improved.
In one embodiment, the distance between sense electrode 40 and working electrode 20 is less than 1 μm.
In this embodiment, when the sample solution contains the object to be detected, the sensing electrode 40 and the working electrode 20 start to work to generate the induced potential, but the distance between the sensing electrode 40 and the working electrode 20 cannot be too large, so that the induced potential cannot be generated, the detection result is inaccurate, and the detection accuracy can be improved by setting the distance between the sensing electrode 40 and the working electrode 20 to be less than 1 μm.
In one embodiment, sense electrode 40 and working electrode 20 are each rectangular in configuration.
In one embodiment, sensing electrode 40 and working electrode 20 are each 2CM in length, and biological probe 30 is one quarter of the length of working electrode 20.
In one embodiment, the material of sensing electrode 40 and working electrode 20 is any one of gold, silver, copper, iron, and aluminum.
In this embodiment, by setting the material of the sensing electrode 40 and the working electrode 20 to be a metal material, the induced potential can be better generated, and the accuracy of the detection result can be improved.
In one embodiment, the material of sense electrode 40 and working electrode 20 is gold.
The embodiment of the application also provides electronic equipment, which comprises: a reagent detecting apparatus according to any one of the above.
In this embodiment, by integrating the reagent detection device in the electronic device, when the sample solution is required to be detected, only the sample solution is required to be dripped on the sample substrate 10, the working electrode 20, the sensing electrode 40 and the biological probe 30, after the test is completed, the sample substrate 10, the working electrode 20, the sensing electrode 40 and the biological probe 30 can be removed by separating the working electrode 20, the sensing electrode 40 from the working electrode interface 60 and the sensing electrode interface 70, and since the field effect transistor 80 is not in contact with the sample solution, the field effect transistor 80 can be recycled, and a new sample substrate 10, the working electrode 20, the sensing electrode 40 and the biological probe 30 are required to be replaced for each test, thereby greatly reducing the cost of the test.
In the foregoing embodiments, the descriptions of the embodiments are emphasized, and in part, not described or illustrated in any particular embodiment, reference is made to the related descriptions of other embodiments.
The above embodiments are only for illustrating the technical solution of the present application, and are not limiting thereof; although the present application has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present application, and are intended to be included in the scope of the present application.
Claims (10)
1. A reagent testing device, comprising:
a sample substrate for receiving a sample solution;
the working electrode is arranged on the sample substrate;
a biological probe arranged on the working electrode;
the induction electrode is arranged on the sample substrate, and the induction electrode and the working electrode are not contacted with each other;
the power interface is used for accessing pulse voltage;
a working electrode interface;
the induction electrode interface and the working electrode interface are used for respectively connecting the induction electrode and the working electrode;
and the drain electrode of the field effect transistor is connected with the power interface, and the grid electrode of the field effect transistor is connected with the induction electrode interface.
2. The reagent detecting apparatus according to claim 1, wherein the reagent detecting apparatus further comprises:
and the testing circuit is connected with the source electrode of the field effect transistor and is used for detecting the current output by the source electrode of the field effect transistor to generate a current detection signal and generating a detection signal of the object to be detected according to the current detection signal.
3. The reagent testing device of claim 1, wherein the distance between the working electrode and the sensing electrode is equal to the distance between the working electrode interface and the sensing electrode interface.
4. The reagent testing device of claim 1, wherein the working electrode interface and the sensing electrode interface are female interface, and the female interface is matched with the working electrode and the sensing electrode.
5. The reagent detecting apparatus according to claim 1, wherein the working electrode and the sensing electrode are attached to the sample substrate.
6. The reagent detecting apparatus according to claim 1, wherein the sample substrate is a glass substrate or a plastic substrate.
7. The reagent detecting apparatus according to any one of claims 1 to 6, wherein the sensing electrode and the working electrode are arranged in parallel.
8. The reagent detecting apparatus according to any one of claims 1 to 6, wherein a distance between the sensing electrode and the working electrode is less than 1 μm.
9. The reagent detecting apparatus according to any one of claims 1 to 6, wherein the material of the sensing electrode and the working electrode is any one of gold, silver, copper, iron, and aluminum.
10. An electronic device, comprising: the reagent detecting apparatus according to any one of claims 1 to 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223506432.9U CN219348718U (en) | 2022-12-27 | 2022-12-27 | Reagent detection device and electronic equipment |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202223506432.9U CN219348718U (en) | 2022-12-27 | 2022-12-27 | Reagent detection device and electronic equipment |
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| Publication Number | Publication Date |
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| CN219348718U true CN219348718U (en) | 2023-07-14 |
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| CN202223506432.9U Active CN219348718U (en) | 2022-12-27 | 2022-12-27 | Reagent detection device and electronic equipment |
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