CN215517478U

CN215517478U - On-site detection system for LAMP or RT-LAMP and portable device box

Info

Publication number: CN215517478U
Application number: CN202121453727.7U
Authority: CN
Inventors: 张锦海; 陈文琦; 罗正汉; 韩一芳; 王太武; 胡丹; 叶福强; 陈月; 路大峰; 汪春晖
Original assignee: Eastern Theater Disease Prevention And Control Center Of Pla
Current assignee: Eastern Theater Disease Prevention And Control Center Of Pla
Priority date: 2021-06-29
Filing date: 2021-06-29
Publication date: 2022-01-14
Anticipated expiration: 2031-06-29

Abstract

The present invention relates to an on-site detection system for LAMP or RT-LAMP and a portable device case. The on-site detection system comprises a detection device and a constant temperature detector, wherein the constant temperature detector comprises a shell, the top surface of the shell is provided with a placement groove, and a support is arranged in the placement groove; a metal heating block is arranged in the shell; the bracket is provided with an accommodating space for accommodating the detection device; the front side surface of the shell is provided with a control component; the shell is also internally provided with a battery and a circuit board; the circuit board is provided with a controller; the detection device comprises a fixed plate and a movable plate, and the fixed plate is provided with a reaction tube; the top surface of the fixed plate is provided with a mounting part, and the two ends of the movable plate are in sliding connection with the corresponding mounting parts; the movable plate is provided with a washing hole, and an FTA card is arranged in the washing hole; the bottom surface of the movable plate is also provided with a color development component. The portable device case is matched with the on-site detection system. The utility model can directly extract, amplify and display the result of the sample nucleic acid on site, does not depend on expensive automatic instruments, and is suitable for various on-site detection scenes.

Description

On-site detection system for LAMP or RT-LAMP and portable device box

Technical Field

The utility model relates to a field detection system for LAMP or RT-LAMP and a portable device box, belonging to the technical field of nucleic acid detection auxiliary devices.

Background

The main clinical manifestations of some infectious diseases with the same syndrome (such as viral hemorrhagic fever series with fever with hemorrhage, viral rash series with fever with eruption and the like) are similar, the early stage is difficult to identify and diagnose, the final cause confirmation depends on the cause detection, and the screening and the definite diagnosis as soon as possible are crucial to control the epidemic situation of the infectious diseases. Because of the sudden and rapid spreading of the epidemic situation of the infectious disease, the rapid pathogen detection can be preferably carried out on site at the first time, and because the patient specimen or the insect-borne specimen is rolled from the site or the field and then sent to a laboratory with conditions, the distance is long, the time consumption is long, the treatment and the prevention and the control are delayed, the specimen is volatile and can cause false negative, and in addition, certain transmission hidden troubles exist. However, the existing field detection means for infectious disease pathogens are relatively thin, mainly take colloidal gold test strips, have the problems of low sensitivity, narrow detection spectrum, hook effect and the like, and often cause false negative.

Nucleic acid detection is an early, reliable, sensitive method. However, the conventional PCR and DNA probe hybridization techniques are not suitable for clinical diagnosis due to the problems of high false positive and the like; the real-time fluorescent quantitative PCR technology has high sensitivity and good reliability, is a totally enclosed reaction, but the technology needs a fluorescent quantitative PCR instrument with high price, and often needs a fluorescent labeled probe to ensure high specificity, the detection cost is higher, and the precision expensive instrument has higher requirements on quality control, operation environment and professional ability of personnel, is difficult to popularize and use in basic health departments and epidemic prevention institutions, and is also difficult to be directly used for field detection.

Compared with the detection means, the LAMP (loop-mediated isothermal amplification) or RT-LAMP (reverse transcription loop-mediated isothermal amplification) method can better screen early pathogeny on site.

The LAMP/RT-LAMP method has higher specificity and anti-interference capability, and can amplify only when the primer group is matched with 6-8 regions of the target fragment; the reaction system is stable and reliable, is still stable after being placed for 2 weeks at room temperature, is still insensitive to irrelevant and interfering fragments of original or pollution in the sample, and cannot be realized by other similar technologies; high sensitivity, fast amplification, high efficiency, and product amount up to 10 in 1 hr¹⁰The reverse transcription reaction and LAMP amplification can be carried out simultaneously, and the amplification efficiency and the detection sensitivity are about 10 to 100 times higher than those of the common RT-PCR; the result identification is relatively simple and convenient, whether the amplification is carried out or not can be judged by detecting the precipitation turbidity of the reaction tube, the color change after the reaction can be judged by directly observing the color change of a wax-sealed (high melting point) SYBR fluorescent dye at the tube cover or the tube bottom, or the color development can be greatly enhanced by directly adding a hydroxynaphthol blue dye (HNB) which does not influence the amplification efficiency into the reaction system. In a word, the LAMP/RT-LAMP method has lower requirements on sample treatment, operation technology and instruments and equipment, and is more suitable for being used in the scenes of field detection, medical institutions (such as various small and medium hospitals, community hospitals and the like), Point of care testing (POCT) and the like.

The research team of the inventor has devoted long to the research of pathogen detection of infectious diseases, and has applied many patents related to the "integrated test strip for LAMP or RT-LAMP" in 2019. Further research results have been reported by the inventor's research team and reported in this patent.

The utility model patent No. CN202021100924.6 and publication No. CN212549623U disclose a portable charging constant temperature heating device, the bottom of the right side of the outside of the main machine is provided with a USB interface and a switch, the front side is provided with an operation panel, and the USB interface is provided with a transmission line and a voltage converter; the inside bottom of host computer is provided with rechargeable lithium cell and the heating device that can charge, and rechargeable lithium cell and the top of heating device are equipped with the heating bath that can put into heating module or flat cell culture board. The portable charging constant-temperature heating device can heat a detection sample. However, this solution is not directly applicable to on-site detection of LAMP or RT-LAMP.

SUMMERY OF THE UTILITY MODEL

The utility model aims to: aiming at the problems in the prior art, the field detection system for LAMP or RT-LAMP is provided, can directly extract, amplify and display the result of the sample nucleic acid on the field, gets rid of the dependence on expensive automatic instruments, and is particularly suitable for field control of infectious diseases, clinical departments of hospitals and port inspection and quarantine. Meanwhile, a portable device box matched with the on-site detection system is also provided.

In order to achieve the above purpose, the technical scheme of the utility model is as follows:

the field detection system for LAMP or RT-LAMP comprises an LAMP or RT-LAMP detection device and a constant temperature detector, and is characterized in that the constant temperature detector comprises a shell, a placement groove is formed in the top surface of the shell, and a support is arranged in the placement groove; a metal heating block is arranged in the shell, penetrates through the bottom of the placement groove and is positioned below the support; the bracket is provided with a containing space for arranging an LAMP or RT-LAMP detection device; the front side surface of the shell is provided with a control assembly, and the control assembly comprises a display screen, a power supply button and a group of control buttons; a battery and a circuit board are also arranged in the shell; the battery is electrically connected with the circuit board; the circuit board is electrically connected with the metal heating block, the display screen, the power supply button and the control button respectively; the circuit board has a controller;

the LAMP or RT-LAMP detection device comprises a fixed plate and a movable plate, wherein the fixed plate is provided with a row of reaction tubes penetrating through the fixed plate, the upper ends of the reaction tubes are open and are positioned on the top surface of the fixed plate, and the lower ends of the reaction tubes are closed; the bottom surface of the fixed plate is also provided with a supporting piece; the top surface of the fixed plate is provided with a pair of mounting parts, and two ends of the movable plate are respectively connected with the corresponding mounting parts in a sliding manner through slide rail assemblies; the movable plate is provided with a row of flushing holes penetrating through the movable plate, FTA cards are respectively arranged in the flushing holes, first sealing rings are respectively arranged at the bottoms of the flushing holes, and the first sealing rings are abutted to the top surface of the fixed plate; the bottom surface of the movable plate is also provided with a row of second sealing rings and a color development assembly which is arranged in a long strip shape.

In the system, the LAMP or RT-LAMP detection device can concentrate all LAMP or RT-LAMP detection processes into one set of device, and can detect a plurality of samples or a plurality of target nucleic acids at one time; the matched constant temperature detector can provide a field reaction environment for the LAMP or RT-LAMP detection device, thereby thoroughly getting rid of the dependence on expensive automatic instruments, and is particularly suitable for field detection scenes such as infectious disease field prevention and control, hospital clinical departments, port inspection and quarantine and the like.

The constant temperature detector mainly utilizes the metal heating block, the control assembly and the controller, and can provide a metal bath constant temperature reaction environment for the detection device; the metal heating block can be controlled by the control component through the controller to heat.

The reaction tube of the LAMP or RT-LAMP detection device is internally provided with amplification reaction reagent freeze-dried powder (namely LAMP or RT-LAMP detection reagent and amplification primer) in advance, and the color developing component is internally provided with color developing agent in advance; when in use, the complex solution is added into the reaction tube to form a reaction system; the movable plate can slide along the mounting part, and the corresponding part can slide to the target position to correspond to the operation of each detection stage of LAMP or RT-LAMP, so that the whole LAMP or RT-LAMP detection process is integrated in one set of device.

For example: when the sample is added, the detection device is taken, the first sealing ring at the bottom of the flushing hole of the detection device is abutted against the top surface of the fixing plate to seal the bottom of the flushing hole, the second sealing ring of the movable plate is aligned with the upper end opening of the corresponding reaction tube to seal the reaction tube, and the FTA card in the flushing hole of the movable plate is used for extracting the nucleic acid of the sample; when a reaction system is established, firstly, sliding the movable plate to enable the flushing holes to be aligned with the openings at the upper ends of the corresponding reaction tubes, enabling the corresponding reaction tubes to be in sealed communication with the flushing holes through the first sealing rings at the bottoms of the flushing holes, then poking the FTA card into the reaction tubes, and then adding the redissolution with a preset volume; when the device is used for constant temperature reaction, firstly, the movable plate is slid to enable the first sealing ring at the bottom of the flushing hole to be abutted against the top surface of the fixed plate so as to enable the bottom of the flushing hole to be sealed again, and the second sealing ring of the movable plate is aligned to the opening at the upper end of the corresponding reaction tube again so as to enable the reaction tube to be sealed, and then the device is placed into a constant temperature detector for reaction; when color development is carried out, the detection device is taken out of the constant temperature detector, the movable plate is firstly slid to enable the color development component to be aligned to the opening at the upper end of the reaction tube, then the detection device is inverted to enable the reaction liquid to dissolve the color development agent, and then the detection result is judged according to the color, so that visual judgment is realized.

The technical scheme of the utility model is further perfected as follows:

preferably, the controlled end of the metal heating block is connected with the control end of the controller through a circuit board; the signal input end of the display screen is connected with the signal output end of the controller through the circuit board; and the signal output end of the control button is connected with the signal input end of the controller.

With this preferred embodiment, the electrical connection structure between the electronic components can be further optimized.

Preferably, the bottom of the bracket is provided with a through hole for accommodating the top surface of the metal heating block; the metal heating block is provided with a temperature sensor which is electrically connected with the circuit board.

More preferably, the signal output end of the temperature sensor is connected with the signal input end of the controller through the circuit board.

By adopting the preferable scheme, the arrangement structure and the temperature control structure of the metal heating block can be further optimized.

Preferably, a matched upper cover is arranged outside the placing groove; the metal heating block is made of aluminum metal or aluminum alloy; the control buttons comprise a start-stop button, an upper arrow button, a lower arrow button, a temperature button, a time button and a program button; the constant temperature detector also comprises a power interface matched with an external power adapter, and the power interface is electrically connected with the circuit board.

By adopting the preferred scheme, the specific structure of the constant temperature detector can be further optimized.

Preferably, in the LAMP or RT-LAMP detection device, the color development assembly is provided with a strip-shaped bottom plate and a row of color development cups, the color development cups are arranged on the bottom surface of the bottom plate, and the openings of the color development cups face to the fixing plate; the top surface of the bottom plate is provided with a group of push rods, and the push rods penetrate through and are exposed out of the top surface of the movable plate;

the rows of the reaction tubes, the rows of the flushing holes, the rows of the second sealing rings and the rows of the color development cups are parallel to each other; the sliding direction of the movable plates is vertical to each row; the flushing holes, the second sealing ring and the color developing cup are respectively in one-to-one correspondence with and matched with the reaction tubes; on the movable plate, the rows of the flushing holes and the rows of the color-developing cups are positioned at two sides of the rows of the second sealing rings.

More preferably, the slide rail assembly is formed by matching a rail and a slide block, the slide block is arranged on the movable plate when the rail is arranged on the mounting part, and the slide block is arranged on the mounting part when the rail is arranged on the movable plate;

the flushing holes are internally provided with sleeves respectively, two ends of each sleeve are provided with openings, one end of each sleeve is positioned in the flushing hole, the other end of each sleeve penetrates through the top surface of the movable plate and is positioned outside the flushing hole, and the other end of each sleeve is provided with a pipe cover; part or all of the edges of the FTA cards are fixedly connected with the corresponding sleeves; the arrangement direction of the FTA cards is perpendicular to the axial direction of the flushing holes.

More preferably, the distance between the bottom end of the supporting member and the bottom surface of the fixing plate is greater than or equal to the distance between the lower end of the reaction tube and the bottom surface of the fixing plate; the top surface of the fixing plate is also provided with a reagent pipe frame; the first sealing ring and the second sealing ring are respectively fluorine rubber O-shaped rings; the mounting member is a plate.

By adopting the preferable scheme, the specific structure of the LAMP or RT-LAMP detection device can be further optimized.

The utility model also proposes:

a portable device case of an on-site detection system for LAMP or RT-LAMP comprises a case body and a case cover, wherein the case body is internally provided with contents, and the portable device case is characterized in that the contents are provided with grooves matched with the on-site detection system for LAMP or RT-LAMP.

Preferably, the grooves comprise a constant temperature detector holding groove, an LAMP or RT-LAMP detection device holding groove, a pipettor holding groove, a sample adding suction head box holding groove and a reagent tube holding groove; the box body and the box cover are respectively provided with magnetic suction devices which are matched with each other.

The portable device box is specially used for the field detection system, is convenient to carry, and is beneficial to rapidly developing detection actions on the field.

After the utility model is adopted, workers can directly extract, amplify and display the result of the sample nucleic acid on site, the dependence on expensive automatic instruments is eliminated, and the utility model is particularly suitable for the site prevention and control of infectious diseases, clinical departments of hospitals and port inspection and quarantine.

Drawings

The utility model will be further described with reference to the accompanying drawings.

Fig. 1 is a schematic view of the overall structure of embodiment 1 of the present invention.

Fig. 2 is an explosion structure diagram of the constant temperature detector in embodiment 1 of the present invention.

FIG. 3 is a schematic diagram of the entire detecting apparatus according to embodiment 1 of the present invention.

Fig. 4 is an exploded view of the detecting device in example 1 of the present invention.

FIG. 5 is a schematic view of a flushing hole sleeve of the detecting device in embodiment 1 of the present invention.

Fig. 6 is an electrical schematic diagram of the constant temperature detector in embodiment 1 of the present invention.

Fig. 7 to 10 are schematic circuit structures of a part of the constant temperature detector according to embodiment 1 of the present invention.

Fig. 11 is a schematic structural diagram of embodiment 2 of the present invention.

Detailed Description

Example 1

As shown in fig. 1 to 10, the field detection system for LAMP or RT-LAMP of the present embodiment includes a LAMP or RT-LAMP detection device 01 and a constant temperature detector 02, where the constant temperature detector 02 includes a housing 11, a placement groove 12 is disposed on a top surface of the housing 11, and a support 13 is disposed in the placement groove 12; a metal heating block 14 is arranged in the shell 11, and the metal heating block 14 penetrates through the bottom of the placing groove 12 and is positioned below the bracket 13; the bracket 13 is provided with a containing space for arranging the LAMP or RT-LAMP detection device 01; the front side surface of the shell 11 is provided with a control component, and the control component comprises a display screen 15, a power button 16 and a group of control buttons 17; the shell 11 is also internally provided with a battery 18 and a circuit board 19; the battery 18 is electrically connected with the circuit board 19; the circuit board 19 is electrically connected with the metal heating block 14, the display screen 15, the power button 16 and the control button 17 respectively; the circuit board 19 has a controller; the exterior of the placing groove 12 is provided with a matched upper cover 03.

The LAMP or RT-LAMP detection device 01 comprises a fixed plate 21 and a movable plate 22, wherein the fixed plate 21 is provided with a row of reaction tubes 23 penetrating through the fixed plate 21, the upper ends of the reaction tubes 23 are open and positioned on the top surface of the fixed plate 21, and the lower ends of the reaction tubes 23 are closed; the bottom surface of the fixed plate 21 is also provided with a supporting piece 24; the top surface of the fixed plate 21 is provided with a pair of mounting parts 25, and two ends of the movable plate 22 are respectively connected with the corresponding mounting parts 25 in a sliding manner through a sliding rail assembly A; the movable plate 22 is provided with a row of flushing holes 26 penetrating through the movable plate 22, FTA cards 35 are respectively arranged in the flushing holes 26, and first sealing rings are respectively arranged at the bottoms of the flushing holes 26 and are abutted against the top surface of the fixed plate 21; the bottom surface of the movable plate 22 is further provided with a row of second sealing rings 27 and a color development assembly 28 arranged in an elongated shape.

In the constant temperature detector 02, the controlled end of the metal heating block 14 is connected with the control end of the controller through the circuit board 19; the signal input end of the display screen 15 is connected with the signal output end of the controller through a circuit board 19; the signal output terminal of the control button 17 is connected with the signal input terminal of the controller. Further, the control buttons 17 include a Start/Stop button (Start/Stop key), an up arrow button, a down arrow button, a temperature button (Temp key), a Time button (Time key), a program button (P key), and the like; the instrument further includes a power interface (not shown) that mates with an external power adapter and is electrically connected to the circuit board.

The bottom of the bracket 13 is provided with a through hole for accommodating the top surface of the metal heating block 14; the metal heating block 14 has a temperature sensor (not shown) electrically connected to the circuit board 19. The signal output end of the temperature sensor is connected with the signal input end of the controller through a circuit board 19. In addition, the material of the metal heating block 14 is aluminum metal or aluminum alloy.

In the LAMP or RT-LAMP detection device 01, the color development assembly 28 has an elongated base plate 29 and a row of color development cups 30, the color development cups 30 are disposed on the bottom surface of the base plate 29, and the color development cups 30 are opened toward the fixing plate 21; the top surface of the bottom plate 29 is provided with a set of push rods 31, and the push rods 31 penetrate through and expose the top surface of the movable plate 22.

The rows of reaction tubes 23, the rows of flushing holes 26, the rows of second sealing rings 27 and the rows of color developing cups 30 are parallel to each other; the sliding direction of the movable plates 22 is perpendicular to each row; the flushing holes 26, the second sealing rings 27 and the color developing cups 30 are respectively corresponding to and matched with the reaction tubes 23 one by one; on the movable plate 22, the rows of the washing holes 26 and the rows of the color cups 30 are located on both sides of the row of the second sealing rings 27.

The slide rail assembly a is formed by matching a rail 32 and a slide block 33, the slide block 33 is arranged on the movable plate 22 when the rail 32 is arranged on the mounting part 25, and the slide block 33 is arranged on the mounting part 25 when the rail 32 is arranged on the movable plate 22; in this embodiment, the rail 32 is disposed on the mounting member 25, and the slider 33 is disposed on the movable plate 22.

The flushing holes 26 are respectively provided with a sleeve 34, two ends of the sleeve 34 are open, one end of the sleeve 34 is positioned in the flushing hole 26, the other end of the sleeve 34 penetrates through the top surface of the movable plate 22 and is positioned outside the flushing hole 26, and the other end of the sleeve 34 is respectively provided with a pipe cover (not shown); part of the edge or the whole edge of the FTA card 35 is fixedly connected with the corresponding sleeve 34; the FTA card 35 is disposed in a direction perpendicular to the axial direction of the flushing port 26.

The distance between the bottom end of the supporting member 24 and the bottom surface of the fixed plate 21 is greater than or equal to the distance between the lower end of the reaction tube 23 and the bottom surface of the fixed plate 21; the top surface of the fixing plate 21 is also provided with a reagent tube holder 36 (for example, for placing an EP tube containing a reagent); the first sealing ring and the second sealing ring 27 are made of fluorine rubber O-shaped rings respectively; the seating part 25 is a plate.

The specific use process of the system of the embodiment is as follows:

1. sample application

The LAMP or RT-LAMP detection device 01 is taken, and the first sealing ring at the bottom of the washing hole 26 is abutted against the top surface of the fixing plate 21 to seal the bottom of the washing hole 26, and the second sealing ring 27 of the movable plate 22 is aligned with the upper end opening of the corresponding reaction tube 23 to seal the same.

About 5-10. mu.l of the sample to be tested is pipetted onto FTA card 35 in wash well 26. If an excessive sample such as 1 drop is dropped, the excessive liquid outside the FTA card 35 in the hole needs to be sucked and discarded as completely as possible (at this time, a stainless steel waste liquid tank with a built-in disinfection liquid is recommended to be additionally provided). After loading, a few minutes are recommended, and after the sample is sufficiently inactivated in the FTA card, the card is slightly dried in the shade. If necessary, the product can be placed into a multilayer protective bag containing a desiccant.

2. First time of washing

80 to 200. mu.l of FTA purification reagent (i.e., FTA extract, Whatman product No. WG120204) was added to the washing well 26, and the mixture was allowed to stand for 5min and discarded by pipetting. Repeat 3 times. Care is taken to avoid poking the FTA card 35.

3. Second rinsing

80 to 200. mu.l of TE buffer (10mM Tris-HCl, 0.1mM EDTA, pH 8.0) was added to the washing well 26, and the mixture was allowed to stand for 5min and discarded. Repeat 3 times. Care is taken to avoid poking the FTA card 35. The FTA card 35 may optionally be dried or air dried thereafter when the FTA card 35 is dried.

Note: in the above-mentioned nucleic acid extraction process, because the first sealing washer seals the bottom of the flushing hole 26, and in addition, the volume of the liquid added each time in the nucleic acid extraction process is small and is absorbed in time, it can be ensured that liquid leakage caused by adding liquid can not occur when the FTA card 35 is used for nucleic acid extraction.

4. Push-pull movement of one

The movable plate 22 is pushed upward to slide along the seating member 25 so that the flushing holes 26 are aligned with the upper end openings of the corresponding reaction tubes 23, and the first sealing ring at the bottom of each flushing hole 26 sealingly communicates the corresponding reaction tube 23 with the flushing hole 26.

The FTA card 35 is gently poked down with a clean pipette tip and dropped into the reaction tube 23.

5. Building system

A25. mu.l reaction system was prepared. The detection experiment is provided with a sample hole to be detected, a negative control hole and a positive control hole. Adding 25 mul of complex solution into each sample hole to be detected; the negative control hole is 22.5 mul of double solution and 2.5 mul of negative control solution; the positive control wells were 22.5. mu.l of reconstituted solution and 2.5. mu.l of positive control. It is recommended that 30. mu.l of paraffin oil be added to each tube to prevent evaporation of the system.

6. Push-pull moving device

The movable plate 22 is pushed down to slide along the mounting member 25, so that the first sealing ring at the bottom of the flushing hole 26 abuts against the top surface of the fixed plate 21 to make the bottom of the flushing hole 26 sealed again, and the second sealing ring 27 of the movable plate 22 is aligned with the upper end opening of the corresponding reaction tube 23 again to make it sealed.

7. Isothermal amplification

Putting the whole device into a constant temperature detector 02 for amplification reaction (65 ℃, 60 min); and taking out the device after the reaction is finished.

8. Push-pull moving device

The movable plate 22 is pushed down to slide along the mounting member 25 so that the color developing cup 30 of the color developing assembly 28 is aligned with the upper end opening of the reaction tube 23.

9. Color development and observation

The push rod 31 is pushed down to push the color cup 30 into the reaction tube 23 through the bottom plate 29, and the outer wall of the color cup 30 is connected with the inner wall of the reaction tube 23 in a sealing way. The reaction solution was dissolved and mixed with a color developer (SYBR green powder) in the color cup 30 by inverting the apparatus. Thereafter, the reaction solution was observed for color. It is recommended to observe against a black background.

10. Determination of results

And judging the detection result according to a preset judgment standard.

If the negative control reaction tube is orange or light red and the positive control reaction tube is green, the detection is effective, and the detection result of each sample to be detected is continuously judged: if the reaction tube of the sample to be detected is green, the sample to be detected is positive, and if the reaction tube of the sample to be detected is orange or light red, the sample to be detected is negative.

If the negative and positive control reaction tube does not accord with the result, the detection result is invalid, and the detection is required to be carried out again.

An example of the use process of the constant temperature detector in the embodiment is as follows:

the constant temperature detector can be directly used or charged through an external power adapter, and can also be used within a preset time period by depending on a built-in battery.

(1) The power-on is carried out by pressing the left power key of the front panel. And self-checking the equipment and displaying the current temperature.

(2) Press Start/Stop key and enter program interface, default to P1 program (drying filter paper sheet 65 deg.C, 5 min). Then, the Start/Stop key is pressed to run the program. If the program needs to be stopped halfway, the Start/Stop key is pressed until the program initial interface is returned.

(3) In the program interface, the program can be selected by pressing a P key and then pressing an up-down arrow button, for example, the program P2 is selected by pressing an up arrow key (the amplification reaction is carried out at 65 ℃, 60min), and then the program is run by pressing a Start/Stop key.

(4) Modification of reaction time and temperature: the program is selected to be modified, the Time value and the Time unit (such as H, M, S) can be switched in sequence by pressing the Time key, and the Time value or the Time unit can be changed by pressing the up-down arrow key. Pressing the Temp key selects the temperature, and pressing the up and down arrow keys changes the temperature value.

The electric components of the present embodiment are each constituted by conventional electronic components. The present embodiment relates only to the use of a software control program and there is no improvement to the software control program itself.

Example 2

As shown in FIG. 11, the portable device case for the in-situ detection system of LAMP or RT-LAMP of the present embodiment comprises a case body 04 and a case cover 05, wherein the case body 04 is provided with a content 41, and the content 41 is provided with a groove matching with the in-situ detection system for LAMP or RT-LAMP of embodiment 1.

Specifically, the grooves include a constant temperature detector holding groove 42, a LAMP or RT-LAMP detection device holding groove 43, a pipette holding groove 44, a sample addition tip cassette holding groove 45, and a reagent tube holding groove 46; the box body 04 and the box cover 05 are respectively provided with magnetic attracting devices (not shown) matched with each other.

The portable device box is specially used for the field detection system in the embodiment 1, the length, the width and the height of the outer size are 420 x 336 x 153mm, the portable device box is convenient to carry, can be lifted by a single hand to follow, and is beneficial to rapidly developing detection actions on the field.

In addition to the above embodiments, the present invention may have other embodiments. All technical solutions formed by adopting equivalent substitutions or equivalent transformations fall within the protection scope of the claims of the present invention.

Claims

1. The field detection system for LAMP or RT-LAMP comprises an LAMP or RT-LAMP detection device and a constant temperature detector, and is characterized in that the constant temperature detector comprises a shell, a placement groove is formed in the top surface of the shell, and a support is arranged in the placement groove; a metal heating block is arranged in the shell, penetrates through the bottom of the placement groove and is positioned below the support; the bracket is provided with a containing space for arranging an LAMP or RT-LAMP detection device; the front side surface of the shell is provided with a control assembly, and the control assembly comprises a display screen, a power supply button and a group of control buttons; a battery and a circuit board are also arranged in the shell; the battery is electrically connected with the circuit board; the circuit board is electrically connected with the metal heating block, the display screen, the power supply button and the control button respectively; the circuit board has a controller;

2. The field detection system for LAMP or RT-LAMP according to claim 1, characterized in that the controlled end of the metal heating block is connected with the control end of the controller via a circuit board; the signal input end of the display screen is connected with the signal output end of the controller through the circuit board; and the signal output end of the control button is connected with the signal input end of the controller.

3. The in-situ detection system for LAMP or RT-LAMP as claimed in claim 1, wherein the bottom of the bracket is provided with a through hole for accommodating the top surface of the metal heating block; the metal heating block is provided with a temperature sensor which is electrically connected with the circuit board.

4. The in-situ detection system for LAMP or RT-LAMP as claimed in claim 3, wherein the signal output terminal of the temperature sensor is connected to the signal input terminal of the controller via a circuit board.

5. The field detection system for LAMP or RT-LAMP according to claim 1, characterized in that the outside of the placement tank is provided with a matching upper cover; the metal heating block is made of aluminum metal or aluminum alloy; the control buttons comprise a start-stop button, an upper arrow button, a lower arrow button, a temperature button, a time button and a program button.

6. The in-situ detection system for LAMP or RT-LAMP according to any one of claims 1 to 5, characterized in that in the LAMP or RT-LAMP detection device, the color development assembly has an elongated base plate and a row of color development cups, the color development cups are placed on the bottom surface of the base plate, and the openings of the color development cups face the fixing plate; the top surface of the bottom plate is provided with a group of push rods, and the push rods penetrate through and are exposed out of the top surface of the movable plate;

7. The in-situ detection system for LAMP or RT-LAMP as claimed in claim 6, wherein the slide rail assembly is constituted by a rail and a slider in cooperation, the slider is provided to the movable plate when the rail is provided to the mounting part, and the slider is provided to the mounting part when the rail is provided to the movable plate;

8. The in-situ detection system for LAMP or RT-LAMP according to claim 7, wherein the distance between the bottom end of the support member and the bottom surface of the fixed plate is greater than or equal to the distance between the lower end of the reaction tube and the bottom surface of the fixed plate; the top surface of the fixing plate is also provided with a reagent pipe frame; the first sealing ring and the second sealing ring are respectively fluorine rubber O-shaped rings; the mounting member is a plate.

9. A portable device case for an on-site detection system for LAMP or RT-LAMP comprising a case body in which contents are provided, characterized in that the contents have a groove that fits the on-site detection system for LAMP or RT-LAMP according to any one of claims 1 to 8.

10. The portable device case for an on-site detection system of LAMP or RT-LAMP according to claim 9, wherein the grooves comprise a constant temperature detector holding tank, a LAMP or RT-LAMP detection device holding tank, a pipette holding tank, a tip holding tank, and a reagent tube holding tank.