CN207512174U - A kind of detection of nucleic acids card - Google Patents
A kind of detection of nucleic acids card Download PDFInfo
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- CN207512174U CN207512174U CN201721028233.8U CN201721028233U CN207512174U CN 207512174 U CN207512174 U CN 207512174U CN 201721028233 U CN201721028233 U CN 201721028233U CN 207512174 U CN207512174 U CN 207512174U
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Abstract
The utility model is related to foranalysis of nucleic acids detection field, spy is related to a kind of detection of nucleic acids card.The utility model sampling unit includes card body, nucleic acid is collected test paper, well and sealed membrane, well and is arranged on card body, and nucleic acid collects test card on well, and well can be sealed film closing；Nucleic acid collects test paper and contains cell cracking agent, detection reagent item is set gradually and is formed by sample pad, bonding pad, NC films and water absorption pad, NC films are C lines, the supporting body of T lines, water absorption pad is used for the flushing after nuclease assay reaction, C lines are control line, T lines are detection line, and different nucleic acid specificity antibody is coated with respectively on C lines, T lines.Using DNA, stringent complementary pairing reacts the utility model in itself, greatly reduces amplification cost, improves amplification efficiency and the degree of automation, suitable for fields such as experimental study, clinical detection, food safety detections.
Description
Technical field
It is specifically a kind of based on nucleic acid amplification reaction and viscosity end the utility model is related to foranalysis of nucleic acids detection field
Hold the nucleic acid detection apparatus and its application method of the strand replacement reaction of mediation.
Background technology
SARS is broken out in most of china area within 2003, and the epidemic spreads of bird flu in 2005 and 2014 are non-
Ebola's epidemic situation of high lethality is wreaked havoc in continent, a series of this infectious disease caused by virus is taught that in the development history of the mankind
The mortality that virus is brought is constantly subjected to threaten.The propagation of virus and the variation well beyond mankind's, it is expected that timely and accurately
Diagnosis virus is for improving patient survival, and control epidemic situation is propagated and effectively disease treatment has very great meaning.
Conventional the pathogenic microorganism examination method is using Vitro Culture Techniques, still, only about 1% bacterium or virus
It can cultivate and the complexity of incubation and very long incubation time, greatly limit the universal of such technology.With point
The development of sub- biology, detection of nucleic acids are applied among viral diagnosis, are not only shortened detection time and are also improved detection
Sensitivity and specificity.Nucleic acid molecules are genetic carrier of all pathogen including virus, pass through the nucleic acid to specificity
It is detected, it can be determined that with the presence or absence of corresponding virus in sample.PCR is most common detection of nucleic acids side
Method, the appearance of the technology make the medicine detection of nucleic acid level enter a completely new stage.Round pcr passes through in different temperatures
Between cycle realize the duplication of DNA molecular, which needs accurately to control temperature, therefore dependent on accurate temperature control device
It is operated with the personnel of profession.In the area of resource scarcity opposite with manpower, PCR method is difficult to use in viral diagnosis.
In order to overcome the dependence to temperature control device, a series of isothermal amplification methods come into being, for example ring mediated isothermal expands
Increase etc..Ring mediated isothermal amplification can expand target nucleic acid at 60-65 DEG C.Isothermal amplification method can be not only one
Kind quick, sensitive, accurate detection of nucleic acids mode, at the same temperature control and detection device are required it is simple, significantly reduce equipment into
This input.It, can be to sample using the cooperation optical detection of DNA fluorescence indicators or the Turbidity measurement based on magnesium pyrophosphate by-product
Product carry out quantitative analysis.
After the strand replacement reaction of cohesive end mediation refers to the single-stranded hybridization of DNA of two complete complementaries or partial complementarity, put
The process of the DNA chain of one or more prehybridization is changed, which is a dynamic competition process.This reaction starts from target
Pendency end, that is, cohesive end of DNA.What TSD was relied on is DNA stringent base pair complementarity properties in itself, does not need to nuclease
Participation can occur.Therefore under conditions of the direct anneal of DNA double chain, which can pass through complementary single-stranded pendency
Region, that is, cohesive end causes, and then undergoes a branch migration process and completes.
In clinical case, same illness may be generated by a variety of different pathogen, cause diagnosis with suiting the medicine to the illness
The difficulty write a prescription.Using nucleic acid amplification method, either PCR or isothermal duplication, how to detect simultaneously multiple pathogens or
Target gene is always a significant challenge in clinical detection.Therefore, invention one kind is easy to operate, accuracy is high, sensitivity
High detection of nucleic acids product realizes that detection is of great significance while multiple pathogens or target gene.
At present, accurate medical treatment has become the research hotspot and development trend of global modern medical service, related in the world precisely to cure
The research and achievements conversion for the treatment of surge forward.Plan to propose to greatly develop accurate medical treatment in China 13 so that in this science and technology
Field China there will be an opportunity to realize that bend is overtaken other vehicles, and walk the rank of advanced units in world's medical science technology.Precisely medical treatment includes accurate
Diagnosis and precisely treatment, wherein diagnosis is the basis for the treatment of, and the diagnosis of the molecule based on nucleic acid detection technique is then precisely to diagnose
Core.China or even in the world current molecule diagnose market and are based primarily upon various large-scale instrument and equipments and the tissue of tediously long complexity
Sample pretreatment means have the shortcomings that costly, time-consuming, target is inaccurate, popularization is not high.The utility model is to be based on
The novel molecular diagnosing chip of microfluidic chip technology, compared with prior-generation technology have high-throughput, low cost, rapidly and efficiently, spirit
The advantages that sensitivity is high, with strong points, universality is strong has very wide application potential and market prospects.Compared with prior art
It can only take the mode of " sweeping entirely " that the full-length genome of diagnosis and treatment object is sequenced, cause the waste of vast resources and the nothing of data
Meaning is repeated so that the present situation that Consumer groups hang back to high sequencing expense.
Utility model content
In view of the deficiencies of the prior art, the strand displacement that the utility model is mediated by nucleic acid amplification reaction or cohesive end
Reaction, not only can visually carry out qualitative analysis also can carry out quantitative analysis by optical detector, realize to single sample or
Multiple pathogens or target gene progress in person's multisample detect simultaneously, detect and take short, accuracy and high sensitivity, and
Nucleic acid amplification enzyme and triphosphate deoxy-nucleotide usually required in nucleic acid amplification reaction are avoided using TSDR reaction principles, profit
With DNA, stringent complementary pairing reacts in itself, greatly reduces amplification cost, improves amplification efficiency and the degree of automation, fits
For fields such as experimental study, clinical detection, food safety detections.
The technical solution of the utility model is：A kind of detection of nucleic acids card, including sampling unit, detection unit, feature exists
In：The sampling unit includes card body, nucleic acid is collected test paper, well and sealed membrane, well and is arranged on card body, nucleic acid
Test card is collected on well, well can be sealed film closing；The nucleic acid collects test paper and contains cell cracking agent,
The detection unit include detection reagent item, the detection reagent item by sample pad, bonding pad, NC films and water absorption pad successively
Setting composition, bonding pad are the carriers of the specific nucleic acid identification segment of nanogold particle label, and sample pad is micro- for carrying
The carrier of amplified production that the strand replacement reaction that amount sample is mediated through nucleic acid amplification reaction or cohesive end obtains, NC films are C
The supporting body of line, T lines, water absorption pad be used for nuclease assay reaction after flushing, C lines be control line, T lines be detection line, C lines, T lines
It is upper to be coated with different nucleic acid specificity antibody respectively.
According to detection of nucleic acids card as described above, it is characterised in that：The detection unit further includes upper shell, lower housing
And insulating cushion, upper shell are provided with detection window, lower housing forepart includes the outpost of the tax office one, the outpost of the tax office two, insulating cushion card slot and water suction paper card
Slot, insulating cushion are placed in insulating cushion card slot, and blotting paper is placed in blotting paper card slot, and the outpost of the tax office is set respectively on the inside of lower housing
First, the outpost of the tax office two, the rear portion of lower housing is bogey, and detection reagent item is arranged on bogey, and upper shell is connected to lower casing
After body, detection window face C lines, T lines.
According to detection of nucleic acids card as described above, it is characterised in that：The card body material is plastics.
According to detection of nucleic acids card as described above, it is characterised in that：The sealing membrane material is plastics, and sealed membrane passes through
Viscose glue or electrostatic interaction are fixed on card body.
According to detection of nucleic acids card as described above, it is characterised in that：The bonding pad and sample pad material are glass fibers
Dimension.
According to detection of nucleic acids card as described above, it is characterised in that：The NC membrane materials are nitrocellulose filter.
According to detection of nucleic acids card as described above, it is characterised in that：Reaction reagent is further included, the reaction reagent is core
Sour amplifing reagent, nucleic acid amplification agents include enzyme, buffer solution, the triphosphate deoxy-nucleotide needed for primer, nucleic acid amplification reaction,
The strand replacement reaction reagent of cohesive end mediation includes primer, fluorogen, biotin.
According to detection of nucleic acids card as described above, it is characterised in that：Buffer solution is further included, the buffer solution delays for phosphoric acid
Fliud flushing.
According to detection of nucleic acids card as described above, it is characterised in that：Detector is further included, the detector is adopted by optics
Collect unit, image-signal processor and electronics peripheral hardware composition, detection of nucleic acids is read as a result, including it in liquid by optical detection
On brilliant screen, and print.
The invention also discloses a kind of application methods of detection of nucleic acids card, include the following steps：
Detection reagent item is placed on the bogey at lower housing rear portion, insulating cushion is placed on insulating cushion card slot,
Blotting paper is placed on blotting paper card slot, upper shell and lower housing are connected together, and card body is sleeved on upper shell and lower housing
Forepart, well is made to be in right over blotting paper card slot；
Test paper is collected to nucleic acid by well and is respectively dropped into sample and buffer solution, is dried after standing, by card body to being pushed forward
To the outpost of the tax office two, well is made to be in right over insulating cushion card slot；
Reaction solution is instilled into well, reaction solution collects test paper with nucleic acid and insulating cushion contacts, then using sealed membrane
Well is closed, and is positioned in baking oven and reacts；
Reaction finishes, and card body is pushed forward to the outpost of the tax office one, and well is made to be in right over sample pad, removes sealed membrane, to
Buffer solution is instilled in well, then observes the variation of C line, T lines.
The beneficial effects of the utility model are：Using a detection device, multiple nucleic acid amplification reaction systems are pre-stored in
In detection of nucleic acids card, the strand replacement reaction that is mediated by nucleic acid amplification reaction or cohesive end, using chromatograph effect, nucleic acid expands
Increase reaction and cohesive end mediation strand replacement reaction, not only can visually carry out qualitative analysis also can by optical detector into
Row quantitative analysis is realized and the multiple pathogens in single sample either multisample or target gene is carried out while detected, examines
It surveys and takes short, accuracy and high sensitivity, and core usually required in nucleic acid amplification reaction is avoided using TSDR reaction principles
Sour amplification enzyme and triphosphate deoxy-nucleotide, using DNA, stringent complementary pairing reacts in itself, greatly reduces amplification cost,
Amplification efficiency and the degree of automation are improved, suitable for fields such as experimental study, clinical detection, food safety detections.
Description of the drawings
Fig. 1 is the utility model upper shell structure diagram；
Fig. 2 is the utility model lower housing structure diagram；
Fig. 3 is detection reagent structure diagram；
Fig. 4 is insulating cushion schematic diagram；
Fig. 5 is sampling unit schematic diagram；
Fig. 6 is sealed membrane schematic diagram；
Fig. 7 is blotting paper schematic diagram.
Reference sign：Upper shell 1, detection window 2, lower housing 3, the outpost of the tax office 1, the outpost of the tax office 25, insulating cushion card slot 6, water suction
Paper card slot 7, nucleic acid collect test paper 8, card body 9, well 10, water absorption pad 11, C lines 12, T lines 13, NC films 14, bonding pad 15, sample
Product pad 16, insulating cushion 17, blotting paper 18, sealed membrane 19.
Specific embodiment
The technical solution of the utility model is described in further detail below in conjunction with attached drawing.
As shown in Figure 1, the detection of nucleic acids card of the utility model includes sampling unit, detection unit, during the test, also
Including matching used reaction reagent, buffer solution and detector.
As shown in figure 5, sampling unit includes card body 9, nucleic acid collects test paper 8, well 10 and sealed membrane 19, well 10
It is arranged on card body 9, nucleic acid is collected test paper 8 and is stuck on well 10, and well 10 can be sealed film 19 and close.In detection process
In, trace sample, reaction solution, buffer solution are added in by well 10, for residue and extra buffer solution, can pass through water suction
Paper 18 is drawn, and after the completion of sample-adding, sealed membrane 19 can be used and seal well 10.9 material of card body of the utility model is modeling
Material, during detection of nucleic acids, card body 9 can be mobile to detection unit direction and by the outpost of the tax office 1 in detection unit or the outpost of the tax office 25
Positioning, to complete the strand replacement reaction and nucleic acid specificity of the nucleic acid amplification reaction of detection of nucleic acids process or cohesive end mediation
Property/non-specific binding process color.
The utility model amplifying nucleic acid collects test paper 8 and contains cell cracking agent, can make protein denaturation simultaneously with lytic cell
Effectively protect nucleic acid from nuclease, oxidant and ultraviolet injury.The utility model nucleic acid is collected can occur core on test paper 8
Sour amplified reaction, the nucleic acid amplification reaction of the utility model can be using DNA as polymerase chain reaction, the ring mediation for expanding template
Isothermal amplification, rolling circle amplification reaction, rely on the isothermal amplification of unwindase, recombinase-mediated isothermal amplification or
Other nucleic acid amplification reactions of person or using RNA as the RT-polymerase chain reaction, reverse transcription loop-mediated etc. of amplification template
Isothermal amplification reaction or other reverse transcription nucleic acid amplification reactions.
19 material of sealed membrane of the utility model can be plastics, and sealed membrane 19 is fixed on card body by viscose glue or electrostatic interaction
On 9, the sealing to well 10 is realized.
As shown in Figures 1 to 4, detection unit includes upper shell 1, lower housing 3, detection reagent item with upper shell clamping
With insulating cushion 17, upper shell 1 is provided with detection window 2, and 3 forepart of lower housing includes the outpost of the tax office 1, the outpost of the tax office 25,6 and of insulating cushion card slot
Blotting paper card slot 7, insulating cushion 17 are placed in insulating cushion card slot 6, and blotting paper 18 is placed in blotting paper card slot 7, under
3 inside of housing sets the outpost of the tax office 1, the outpost of the tax office 25 respectively, and the outpost of the tax office 1, the outpost of the tax office 25 are used to position card body 9.Lower housing 3
Rear portion is bogey, for carrying detection reagent item, after upper shell 1 is connected to lower housing 3, and 2 face C lines 12 of detection window, T lines
13, in this way convenient for directly observation or apparatus measures test result.
As shown in figure 3, the detection reagent item of the utility model is by sample pad 16, bonding pad 15, NC films 14 and water absorption pad 11
Composition is set gradually, bonding pad 15 is the carrier of the specific nucleic acid identification segment of nanogold particle label, and sample pad 16 is to use
In the carrier of amplified production that the strand replacement reaction that carrying trace sample is mediated through nucleic acid amplification reaction or cohesive end obtains,
NC films 14 for C lines 12, T lines 13 supporting body, water absorption pad 11 be used for nuclease assay reaction after flushing, C lines 12 be control line, T
Line 13 is detection line, and in the presence of only C lines 12, testing result is feminine gender, is then detected when C lines 12, T lines 13 exist simultaneously
As a result it is the positive.
16 material of sample pad of the utility model is glass fibre, for carry trace sample through nucleic acid amplification reaction or
The amplified production that the strand replacement reaction of cohesive end mediation obtains, and bonding pad 15 and NC films 14 are directed to through water absorption pad 11, and
It is reacted with specificity coated on C lines 12 coated on NC films 14, T lines 13/non-specific identification nucleic acid fragment；
15 material of bonding pad is glass fibre, and segment is identified for carrying the specific nucleic acid marked through nanogold particle,
To capture the sample nucleic acid fragment amplification product in sample pad 16；
14 material of NC films is nitrocellulose filter, for carrying C lines 12, the T lines 13 that detection of nucleic acids colour developing uses, C lines
12nd, different nucleic acid specificity antibody is coated on T lines 13 respectively, for combining the specific recognition nucleic acid of nanogold particle label
The compound that segment hybridizes with sample nucleic acid amplified production.
The matching used reaction reagent of the utility model can be nucleic acid amplification agents, nucleic acid amplification agents include primer,
Enzyme, buffer solution, triphosphate deoxy-nucleotide needed for nucleic acid amplification reaction, the strand replacement reaction reagent of cohesive end mediation include
Primer, fluorogen, biotin not including the enzyme and triphosphate deoxy-nucleotide needed for nucleic acid amplification, are pre-installed on detection of nucleic acids card
In the containers such as test tube in addition, in case using.
Phosphate buffer etc. may be used in the buffer solution of the utility model, and buffer solution is pre-installed on the examination other than detection of nucleic acids card
In the containers such as pipe, in case using.
The matching used detector of nucleic acid detection apparatus of the utility model, by optically detecting unit, picture signal processing
Device and associated electrical peripheral hardware composition read detection of nucleic acids as a result, it is included on LCD screen, and prints by optical detection
Out
The utility model is described further below by a specific embodiment, but specific embodiment is not
Any restriction is done to the utility model.
Example 1：19 site primer of epithelial growth factor receptor gene in lung Small Cell Lung Cancer
Nucleic acid detection apparatus square is taken out on experimental bench, 1 drop sample is added in into well 10, is stored at room temperature 5 minutes；
1) continue to add in 1-2 drop buffer solutions into well 10, stand after five minutes, card body 9 is pushed forward to the outpost of the tax office 25
Position；
2) add 1 drop reaction solution into well 10, while sealed membrane 19 is closed into well 10, detection device is placed in 65
1 hour or so in DEG C baking oven；
Take out detection device, remove sealed membrane 19, is continued to push forward at the outpost of the tax office 1, be added dropwise 1 drip buffer solution in
In well 10, the variation of C lines 12, T lines 13 is visually can be seen that in 5 minutes.In the presence of only C lines 12, testing result is
Feminine gender, then testing result is the positive when C lines 12, T lines 13 exist simultaneously, and can also be put into the matching used inspection of nucleic acid detection apparatus
It surveys in device and quantitatively detects corresponding result.
The invention also discloses the application methods of detection of nucleic acids card, include the following steps：
Detection reagent item is placed on the bogey at 3 rear portion of lower housing, insulating cushion is placed on insulating cushion card slot 6
17, blotting paper 18 is placed on blotting paper card slot 7, upper shell 1 and lower housing 3 are connected together, and card body 9 is sleeved on
The forepart of housing 1 and lower housing 3 makes well 10 be in right over blotting paper card slot 7；
By well 10 to nucleic acid collect test paper 8 be respectively dropped into sample and buffer solution, dried after standing, by card body 9 to
It is pushed forward to the outpost of the tax office 25, well 10 is made to be in right over insulating cushion card slot 6；
Then reaction solution is instilled into well 10, reaction solution collects test paper 8 with nucleic acid and insulating cushion 17 contacts, then
Well 10 is closed, and be positioned in baking oven and react using sealed membrane 19；
Reaction finishes, and card body 9 is pushed forward to the outpost of the tax office 1, and well 10 is made to be in right over sample pad 16, removal envelope
Membrana oralis 19 instills buffer solution into well 10, then observes the variation of C lines 12, T lines 13.
The micro-fluidic diagnosing chip technology of high throughput that the utility model is taken then has been abandoned background technology and has been used completely
Mode mode, but pointedly target gene segment is expanded with the reaction system of " section type " or signal amplification simultaneously
The ultrasensitiveness detection of direct-reading is carried out, it is achieved thereby that complementing each other with the tentative diagnosis of clinician and be accurate clinic
Diagnosis provides strong reliable foundation, makes precisely to diagnose and precisely medical treatment really becomes a reality.And in emerging pathogen detection
Field, the micro-fluidic molecular diagnosis chip technology of high-throughput high sensitivity of the utility model development are even more to have conventional molecular diagnosis
The incomparable technical advantage of technology.Due to quick expansion of this technology based on the extremely strong pathogenic microorganism genetic fragment of purpose
Increasing rather than " scanning " to pathogenic microorganism full-length genome, so the molecular diagnosis chip quickly can accurately judge pathogen kind
Class and quantity.This achievement by cause China at present still part there is abuse of antibiotics problem thoroughly become history,
The marketization will have immeasurable Social benefit and economic benefit.
The market orientation of the utility model can tentatively be set to：1. pathogen detection.The hair of pathogen detection technology at present
Open up in the ascendant, the problem of abuse of antibiotics is still serious.The utility model can so that the specific aim of clinical application is more accurate,
High degree avoids the economic waste of mistaken diagnosis and patient in medication.2. disease gene detects.Different from being carried out to human body
The mode of full genome sequencing utilizes the micro-fluidic molecular diagnosis chip technology based on high-throughput high sensitivity, clinical gene diagnosis
Huge numbers of families will be entered into gene diagnosis of risk, miniaturization, rapid, economical and practical type portable gene diagnosis instrument will cause
Doctor and patient more understand to its gene risk and disease risk, realize and the specific aim of disease is accurately prevented and treated.
As it will be easily appreciated by one skilled in the art that the above is only the preferred embodiment of the utility model only, not
To limit the utility model, any modification made within the spirit and principles of the present invention, equivalent replacement and change
Into etc., it should be included within the scope of protection of this utility model.
Claims (9)
1. a kind of detection of nucleic acids card, including sampling unit, detection unit, it is characterised in that：The sampling unit include card body,
Nucleic acid is collected test paper, well and sealed membrane, well and is arranged on card body, and nucleic acid collects test card on well, sample-adding
Hole can be sealed film closing；The nucleic acid collects test paper and contains cell cracking agent, and the detection unit includes detection and tries
Agent item, the detection reagent item are set gradually and are formed by sample pad, bonding pad, NC films and water absorption pad, and bonding pad is nanogold
The carrier of the specific nucleic acid identification segment of particle marker, sample pad be for carry trace sample through nucleic acid amplification reaction or
The carrier of amplified production that the strand replacement reaction of cohesive end mediation obtains, NC films are C lines, the supporting body of T lines, and water absorption pad is used for
Flushing after nuclease assay reaction, C lines are control line, and T lines are detection line, and different nucleic acid specificities is coated with respectively on C lines, T lines
Property antibody.
2. detection of nucleic acids card according to claim 1, it is characterised in that：The detection unit further includes upper shell, under
Housing and insulating cushion, upper shell are provided with detection window, and lower housing forepart includes the outpost of the tax office one, the outpost of the tax office two, insulating cushion card slot and water suction
Paper card slot, insulating cushion are placed in insulating cushion card slot, and blotting paper is placed in blotting paper card slot, and setting is closed respectively on the inside of lower housing
Card one, the outpost of the tax office two, the rear portion of lower housing is bogey, and detection reagent item is arranged on bogey, and upper shell is connected to down
After housing, detection window face C lines, T lines.
3. detection of nucleic acids card according to claim 1 or 2, it is characterised in that：The card body material is plastics.
4. detection of nucleic acids card according to claim 1 or 2, it is characterised in that：The sealing membrane material is plastics, is sealed
Film is fixed on by viscose glue or electrostatic interaction on card body.
5. detection of nucleic acids card according to claim 1 or 2, it is characterised in that：The bonding pad and sample pad material be
Glass fibre.
6. detection of nucleic acids card according to claim 1 or 2, it is characterised in that：The NC membrane materials are nitrocellulose
Film.
7. detection of nucleic acids card according to claim 1 or 2, it is characterised in that：Further include reaction reagent, reaction examination
Agent is nucleic acid amplification agents, and nucleic acid amplification agents include primer, the enzyme needed for nucleic acid amplification reaction, buffer solution, triphosphoric acid deoxidation
Nucleotide, the strand replacement reaction reagent of cohesive end mediation include primer, fluorogen, biotin.
8. detection of nucleic acids card according to claim 1 or 2, it is characterised in that：Buffer solution is further included, the buffer solution is
Phosphate buffer.
9. detection of nucleic acids card according to claim 1 or 2, it is characterised in that：Further include detector, the detector by
Optically detecting unit, image-signal processor and electronics peripheral hardware composition, detection of nucleic acids result is read by optical detection.
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| CN201721028233.8U CN207512174U (en) | 2017-08-17 | 2017-08-17 | A kind of detection of nucleic acids card |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201721028233.8U CN207512174U (en) | 2017-08-17 | 2017-08-17 | A kind of detection of nucleic acids card |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107513494A (en) * | 2017-08-17 | 2017-12-26 | 湖北伽诺美生物科技有限公司 | A kind of detection of nucleic acids card and its application method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107513494A (en) * | 2017-08-17 | 2017-12-26 | 湖北伽诺美生物科技有限公司 | A kind of detection of nucleic acids card and its application method |
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