CN1256415A - Chip testing instrument with micro probe array - Google Patents
Chip testing instrument with micro probe array Download PDFInfo
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- CN1256415A CN1256415A CN 99114456 CN99114456A CN1256415A CN 1256415 A CN1256415 A CN 1256415A CN 99114456 CN99114456 CN 99114456 CN 99114456 A CN99114456 A CN 99114456A CN 1256415 A CN1256415 A CN 1256415A
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Abstract
The chip testing instrument consists of casing, frame, test transmission mechanism, probe mechanism, positioning mechanism, cylinder driving mechanism, temperature controlling mechanism, computer and printer. The frame is installed inside the casing, the test transmission mechanism, the positioning mechanism, the cylinder driving mechanism and the temperature controlling mechanism are connected to the frame, the probe mechanism is connected to the Z direction gear of rack of the test transmission mechanism, the sealed casing is connected to the computer via cable, and the printer connected to the computer prints the test results.
Description
A kind of PCR chip testing instrument with micro probe array of the present invention relates to new departure that manufactures and designs of a kind of PCR-of being used for chip testing instrument with micro probe array device, especially utilize optical CCD device, optical fiber detector, pcr chip attemperating unit, detect gearing, locating device, finish the test of PCR microarray probe circulating detection type biological chip polycomponent automatically.
Round pcr mainly is as a kind of selectivity outer-gene amplification method and since through 25～35 take turns the circulation after just can make DNA cloning 10
6Doubly, in scientific research and medical test, be widely used for many years.But, forbidden being used for clinical diagnosis by health ministry from June, 98 because there are defectives such as false positive in PCR.Its main cause is: the mistake that the labile factor that many experiment conditions in the PCR process (primer design and selection, material mixture ratio, reaction time, temperature, cycle period) etc. cause causes producing PCR is drawn and is increased.It two is that the follow-up electrophoretic detection of PCR process can be judged the fragment that whether obtains length-specific, and can't determine its concrete sequence.Its three, PCR reaction is two discrete processes with detecting, complex operation and increased the chance of polluting.Therefore, for testing conditions and equipment limited in, infirmary, the accuracy of the detection of PCR has been subjected to very big influence.In addition, current PC R amplification procedure has very high requirement for technical level of operators and quality.
Biochip mainly is meant by plane Micrometer-Nanometer Processing Technology and supermolecule self-assembling technique, in microanalysis unit and the system that the solid chip surface makes up.Biochip can integrate many difference in functionality devices, for example, the pre-service of biological sample, the extraction of inhereditary material, the amplification of specific gene fragment, biological probe array and Capillary Electrophoresis form whole microfluid system, with realize to compound, protein, nucleic acid, cell and other biological components accurately, fast, the screening or the detection of large information capacity.Genetic chip is a most important class biochip, and it the is integrated gene probe of dense arrangement in a large number can be analyzed a large amount of genes at short notice, makes people can promptly read and analyze the program of life.
Biochip has extremely important meaning on biological detection, medical test, drug screening and gene sequencing.For example in biology, along with molecular biological continuous development, since the Human Genome Project particularly highly visible was implemented, the data of related nucleic acid, protein sequence and structure were exponential increase.And the most challenging work of next century is exactly after the Human Genome Project is finished, and promptly at the back era gene, how we use a large amount of biomolecule information services in human society, and makes medical science, treatment produce basic revolution.In medical science, " the subordinate phase medical science on system, organ, tissue, the cell level " transforms to " on the gene level; DNA → RNA → protein → protein and nucleic acid interaction, and the phase III medical science on they and the environmental interaction level ".This gene diagnosis of carrying out on molecular level and gene therapy will be familiar with the root that disease produces at all, and will be hopeful basic understanding and treat the major disease that comprises cancer.The fundamental change of these biology, medical science, a mensuration and the analysis that basic prerequisite is a gene order.Can carry out gene sequencing and analysis effectively apace, will have influence on the enforcement of the Human Genome Project, thereby influence further developing of biology, medical science.The method that adopted of tradition gene sequencing comprises a series of numerous and diverse steps such as chemical reaction, gel electrophoresis, and these method spended times are longer, and operates complicated, especially time-consuming and be not suitable for portability and check order fast aspect large scale sequencing.Traditional gene order surveying method is being carried out in the improved process, is that the biochip technology of representative arises at the historic moment with the genetic chip.Biochip technology is with many discontinuous analytic process related in the life science, and as specimen preparation, chemical reaction and analyzing and testing etc. are by adopting microelectronics, technologies such as micromechanics are integrated in the chip, make it serialization, integrated, microminiaturization and robotization.The strong means that provide are obtained and analyzed to the maturation of this technology and use and will bring a revolution in medical diagnosis on disease and the life science association areas such as treatment, new drug development, judicial expertise, food and environment of next century for biological information.
Biochip (genetic chip) is an international research focus in recent years always, and advances just with surprising rapidity.There have been many companies to enter the biochip field in the world, worked out PCR and the integrated biochip of DNA array.These systems prepare a PCR micro reaction pool usually on chip, by controlling the temperature cycles of little reaction, carry out gene magnification, in the gene introduced cross pond after then will increasing, with the hybridization of solid phase micro probe array, detect.Microfluidic channel is further made by Affymetrix company then PCR micro reaction pool.Although the existing report that the round pcr chip detection is integrated is characterized in that whole PCR process carries out in a micro reaction pool.Thereby need to be heated up repeatedly, lower the temperature in the same position of device, this can't be incorporated into the action attitude to PCR and follow the tracks of and quantitative test.And the need certain hour that heats up at present, lowers the temperature, prolonged the working time.
The objective of the invention is to have weak point at present PCR detection method, a kind of PCR chip testing instrument with micro probe array is provided, be based on PCR microarray probe circulating detection type biological chip technology and propose a kind of novel PCR chip testing instrument with micro probe array, thereby obtain integrated degree height, PCR detector that automaticity is high.
A kind of PCR chip testing instrument with micro probe array is to adopt following scheme to realize: the PCR chip testing instrument with micro probe array, with round pcr and gene microarray probe technique, detection technique etc. are together integrated, constitute novel PCR and detect diagnostic instrments.A kind of PCR chip testing instrument with micro probe array of the present invention is made up of following part: shell, frame, detection gearing, probe unit, locating device, cylinder actuating device, attemperating unit, computing machine, printer are formed.Frame is whole lays in the enclosure, and detection gearing, locating device, cylinder actuating device link to each other with frame with attemperating unit, and probe unit links to each other with gearing (specifically linking to each other with Z direction tooth bar).The casing of whole casing sealing links to each other with computing machine by cable.View window, power knob, start button, light source button, pneumatic button are arranged on the front panel of shell, light source and rear panel connecting groove are arranged on the rear panel, cylinder actuating device mainly comprises cylinder and stepper motor, cylinder actuating device is installed on the frame, links to each other with the gas circuit of pcr chip by gas circuit.Attemperating unit comprises that the cable of the pad of being with temperature control and sensing, driving usefulness forms, and attemperating unit is installed on the frame.On the temperature control pad, be placed with the PCR sheet, utilize the temperature control sheet to control four humidity provinces such as the annealing of PCR, amplification, extension, hybridization, thereby reach the purpose of each warm area constant temperature.Detect the moving component that gearing mainly comprises X, Y, three directions of Z, X is arranged to stepper motor, X is to ball-screw, and X is to guide rail, and Y is to stepper motor, and Y is to ball-screw, and X is to guide rail, and Z is to stepper motor, and Z is to driven wheel, and Z is to tooth bar.X is installed on the frame to guide rail, and by being fixed on the bearings on the frame, X's X links to each other to ball-screw with X to stepper motor to ball-screw; Y is installed in X on planker to guide rail, and by being fixed on the Y of X on planker to bearings, Y's Y links to each other to ball-screw with Y to stepper motor to ball-screw.Z is fixed on Y on planker to stepper motor, and Z drives Z and moves up and down to tooth bar by gear, tooth bar transmission to stepper motor simultaneously.In transmission process, X drives X to stepper motor and moves to planker, and Y drives Y to stepper motor and moves to planker on fixing and the X planker, fixingly drives Z to the Z of planker to stepper motor with Y and moving up and down to tooth bar, thereby realize three-dimensional transmission.Locating device comprises the limit switch of X, Y, three directions of Z, and X is installed on the frame to limit switch, and Y is installed in X on planker to limit switch, and Z is installed in Y on planker to limit switch.Sniffer comprises CCD observation parts and optical fiber detection part.Sniffer is installed in Z on tooth bar, and by three-dimensional transmission, sniffer is finished three-dimensional motion.In testing process, the position signalling of sniffer, the position signalling of fluid in the pcr chip outputs to the rear panel connecting groove by sensor (limit switch, CCD detection part) and is connected to computer system.A/D card, temperature control hardware circuit plate, graphics card, mainboard, multifunction card, D/A card are installed, simultaneously external display, keyboard, mouse and printer in the computing machine.By A/D card in the machine, with analog signal conversion is digital signal, calculates the value of each signal,, position signalling is through D/A conversion and rear panel connecting groove, control X, Y, Z to stepper motor and cylinder drive motor, thereby can locate the fluorescence signal that obtains the fixed point place.Fluorescence signal in the course of reaction advances computer graphics card by sensor (optical fiber detection part) through input, delivery outlet, and the process image processing obtains the characteristic fluorescence spectral line in the course of reaction, thereby quantitatively determines the result of PCR, and by printer prints.The PCR temperature sensor signal links to each other with computing machine through cable, passes through hardware control circuit or software control, and computing machine outputs to the driving chip of PCR attemperating unit with temperature signal by cable, controls the steady temperature of each warm area.In probe unit, the optical fiber detection part itself has laser generator, receiver, light coupling mechanism.Attemperating unit has ceramic heat-insulation board, ceramic heat-insulation board has the hole, device has the heating and cooling device on the ceramic heat-insulation board, the heating and cooling device can adopt semiconductor heating and cooling device, the heating and cooling device is equipped with heat shield or coating heat shield outward, between heating and cooling device and ceramic heat-insulation board, heat insulation is housed, is provided with little heat transfer sheet, be provided with thermal resistance sensor at little heat transfer sheet front end in heating and cooling device front.During use, the sensing drive unit is installed in the back side of pcr chip, and the position corresponds respectively to four humidity provinces of sex change, annealing, extension, hybridization of pcr chip, heats automatically or refrigeration, to reach the temperature control purpose in different temperatures district.The hardware controls circuit of attemperating unit mainly comprises several parts compositions of extraction, proportional integral, proportion differential and output amplification Drive and Control Circuit of sensor signal.The extraction circuit of sensor signal comprises power supply, resistance R 1 and R2, adjustable resistance R
W1, sensor resistance R
T, the proportion differential circuit comprises operational amplifier A 1, resistance R 4 and R3, adjustable resistance R
W2, the proportional integral circuit comprises operational amplifier A 2, resistance R 5, R6 and R7, adjustable resistance R
W3, amplification driving circuit comprises resistance R 8, diode D1 and D2, triode T1.Signal of sensor is connected to the negative terminal of operational amplifier A 1, compare with the signal of anode, amplify the voltage break-make relay that drives the heating and cooling semiconductor chip by triode T1, thereby reach the purpose of temperature control through proportion differential, integrating circuit output back.
A kind of PCR chip testing instrument with micro probe array of the present invention, both simplified operation steps, shorten the PCR time, raise the efficiency, the reaction system and the external world can be carried out tight effective isolation again, pcr amplification-probe hybridization testing process is integrated, need not carry out electrophoretic analysis, but directly by CCD and microcomputer analysis and provide testing result.Because the characteristic property of probe hybridization, thereby can get rid of electrophoresis and can't get rid of the false positive defective.Sex change in the PCR circulation that main is, annealing, integral body of extension and micro probe array chip formation, sex change, annealing, extension, hybridization temperature are controlled respectively, thereby avoid reacting out temperature, temperature-fall period and because the influence that the temperature control error is brought, make under the guidance of thought at biochip simultaneously, on a biochip, can carry out the detection of several samples.Simultaneously can follow the trail of each round-robin efficient of PCR, carry out the dynamic quantitative analysis.Simultaneously,,, reduce test job intensity, improved the accuracy of analyzing, reduce environmental pollution simultaneously so chip replacing, application of sample, temperature control CCD number are adopted, analyze, printed automatically and carry out because system adopts control automatically.
The invention will be further described below with reference to accompanying drawing.
Fig. 1 detector system diagram of the present invention.
Fig. 2 detector front view of the present invention.
Fig. 3 detector rear view of the present invention.
The inner front view of Fig. 4 detector of the present invention.
The inner left view of Fig. 5 detector of the present invention.
The mechanical assembly drawing of Fig. 6 attemperating unit of the present invention.
The hardware controls wiring diagram of Fig. 7 attemperating unit of the present invention.
With reference to accompanying drawing 1 detector system diagram, the PCR chip testing instrument with micro probe array that the present invention proposes, the sex change of PCR process is carried out in the T1 micro-fluid reactor, anneals and extends among microreactor T2 and the T3 and carry out, and T4 is a micro probe array.Micro probe array can be low-density or high density, single function or the multi-functional array probe that the preparation of point sample method is synthesized or passed through to original position.V1 and V2 are T-valve.Above-mentioned each element (T1, T2, T3, T4, V1, V2) constitutes a PCR-microarray probe circulating detection type chip chip-1, can form chip chip board more than by a plurality of chip-1.Become integrated a plurality of different sample P CR micro probe array cake core systems that can be used for measuring on same chip, utilize attemperating unit and computing machine, the different warm areas of PCR are carried out temperature control.Utilize CCD and optical fiber probe detection part and computer system simultaneously, carry out three-dimensional motion scan and obtain the detection signal that diverse location is pointed out, detection signal is handled the experimental result that obtains PCR, and come out by printer prints.
Referring to accompanying drawing 2 detector front views, front panel is equipped with and comprises power knob, start button, light source button, pneumatic button, view window.
Referring to accompanying drawing 3 detector rear views, rear panel is equipped with the rear panel connecting groove.
Referring to the inner front view of accompanying drawing 4 detectors, the inner left view of accompanying drawing 5 detectors.In conjunction with the accompanying drawings 4 and accompanying drawing 5, PCR detector of the present invention is made up of following part: shell 1, frame 2, detect gearing, probe unit, locating device, cylinder actuating device, attemperating unit, computing machine, printer and form.View window 14, power knob 19, start button 20, light source button 21, pneumatic button 22 are arranged on the front panel of shell 1, light source 11 and rear panel connecting groove 15,16 are arranged on the rear panel, cylinder actuating device mainly comprises cylinder 3 and stepper motor 4, and attemperating unit comprises that the cable of pad 17 compositions of being with temperature control and sensing, driving forms.On temperature control pad 17, be placed with PCR sheet 18, utilize four humidity provinces such as annealing on the temperature control sheet 17 control PCR sheets, amplification, extension, hybridization, thereby reach the purpose of each warm area constant temperature.Detect the moving component that gearing mainly comprises X, Y, three directions of Z, X is arranged to stepper motor 7, X is to ball-screw 6, and X is to guide rail 24, and Y is to stepper motor 25, and Y is to ball-screw 26, and Y is to guide rail, and Z is to stepper motor 9, and Z is to driven wheel 10, and Z is to tooth bar 8.X is installed on the frame to guide rail, and X supports by the bearing 23 that is fixed on the frame to ball-screw 6, and X links to each other to ball-screw 6 with X to stepper motor 9; Y is installed in X on planker to guide rail, and Y supports to bearing 23 by being fixed on the Y of X on planker to ball-screw 26, and Y links to each other to ball-screw 26 with Y to stepper motor 25.Z is fixed on Y on planker to stepper motor 9, and Z drives Z and moves up and down to tooth bar 8 by gear 10, tooth bar 8 transmissions to stepper motor simultaneously.In transmission process, X drives X to stepper motor 7 and moves to planker, and Y drives Y to stepper motor 25 and moves to planker on fixing and the X planker, fixingly drives Zs to the Z of planker to stepper motor 9 with Y and moving up and down to tooth bar 8, thereby realize three-dimensional transmission.Locating device comprises X, Y, three direction limit switches 5 of Z, and X is installed on the frame to limit switch, and Y is installed in X on planker to limit switch, and Z is installed in Y on planker to limit switch.Sniffer comprises CCD observation parts 12 and optical fiber detection part 13.In testing process, the position signalling of detection system, the position signalling of fluid in the pcr chip 18 outputs to rear panel connecting groove 15 by sensor (limit switch 5, CCD detection part 12) and is connected to computer system.A/D card, graphics card, mainboard, multifunction card, D/A card are installed, simultaneously external display, keyboard, mouse and printer in the computing machine.By A/D card in the machine, with analog signal conversion is digital signal, calculate the value of each signal, according to the control principle computing machine temperature signal, position signalling are changed and rear panel connecting groove 16 through D/A, output to the driving chip 28 of PCR temperature control, X to stepper motor 7, Y to stepper motor 25, Z to stepper motor 9 and cylinder drive motor 4, thereby can locate the fluorescence signal that obtains the fixed point place.Fluorescence signal in the course of reaction advances computer graphics card by sensor (optical fiber detection part 13) through input, delivery outlet, and the process image processing obtains the characteristic fluorescence spectral line in the course of reaction, thereby quantitatively determines the result of PCR, and passes through computer printout.The PCR temperature sensor signal links to each other with computing machine through cable, passes through hardware control circuit or software control, and computing machine outputs to the driving chip 28 of PCR attemperating unit with temperature signal by cable, controls the steady temperature of each warm area.Optical fiber detection part 13 itself has laser generator, receiver, light ray coupling device.
Mechanical assembly drawing referring to accompanying drawing 6 attemperating units.Attemperating unit has ceramic heat-insulation board 27, ceramic heat-insulation board has the hole, device has heating and cooling device 28 on the ceramic heat-insulation board, the heating and cooling device can adopt semiconductor heating and cooling device, heating and cooling device 28 outer heat shield or the coating heat shields 29 of being equipped with, heat insulation 30 is housed between heating and cooling device and ceramic heat-insulation board, be provided with little heat transfer sheet 31 in heating and cooling device front, be provided with thermal resistance sensor 32 at little heat transfer sheet front end.During use, the sensing drive installation is at the back side of pcr chip 18, and the position corresponds respectively to four humidity provinces of sex change, annealing, extension, hybridization of pcr chip, heats automatically or refrigeration, to reach the temperature control purpose in different temperatures district.
Hardware controls wiring diagram referring to accompanying drawing 7 attemperating units.The hardware controls circuit of attemperating unit mainly comprises several parts compositions of extraction, proportional integral, proportion differential and output amplification Drive and Control Circuit of sensor signal.The extraction circuit of sensor signal comprises+9 volts and-9 volts of power supplys, resistance R 1 and R2, adjustable resistance R
W1, sensor resistance R
T, the proportion differential circuit comprises operational amplifier A 1, resistance R 4 and R3, adjustable resistance R
W2, the proportional integral circuit comprises operational amplifier A 2, resistance R 5, R6 and R7, adjustable resistance R
W3, amplification driving circuit comprises resistance R 8, diode D1 and D2, triode T1.For the accurate temperature of measuring, the negative terminal of employing comparer A1 connects the voltage of sensor, voltage difference with anode resistance R 3, through proportional integral and proportion differential control, amplification outputs to relay ZJ through triode T1 by resistance R 8, control the time of the voltage break-make of heating and cooling device, thereby reach the purpose of temperature control.
Claims (3)
1. a kind of PCR chip testing instrument with micro probe array according to the present invention, its special sheet is to be made up of shell, frame, detection gearing, probe unit, locating device, cylinder actuating device, attemperating unit, computing machine and printer; Frame is whole lays in the enclosure, and detection gearing, locating device, cylinder actuating device link to each other with frame with attemperating unit, and probe unit links to each other with Z direction tooth bar, and the casing of whole casing sealing links to each other with computing machine by cable; Temperature control hardware circuit plate, mainboard, A/D card, graphics card, multifunction card, D/A card are arranged, the external display of computing machine, keyboard and mouse in the computing machine; Cylinder actuating device mainly comprises cylinder and stepper motor; Attemperating unit comprises that the cable of the pad of being with temperature control and sensing, driving usefulness forms; On the temperature control pad, be placed with the PCR sheet; Detect the moving component that gearing mainly comprises X, Y, three directions of Z, X is arranged to stepper motor, X is to ball-screw, and X is to guide rail, y is to stepper motor, Y is to ball-screw, and Z is to tooth bar, and Z is to stepper motor, Z is to driven wheel, X is installed on the frame to guide rail, and by being fixed on the bearings on the frame, X's X links to each other to ball-screw with X to stepper motor to ball-screw; Y is installed in X on planker to guide rail, Y to ball-screw by being fixed on the Y of X on planker to bearings, Y links to each other to ball-screw with Y to stepper motor, and Z is fixed on Y on planker to stepper motor, and Z drives Z and moves up and down to tooth bar by gear, tooth bar transmission to stepper motor simultaneously; Locating device comprises the limit switch of X, Y, three directions of Z, and X is installed on the frame to limit switch, and Y is installed in X on planker to limit switch, and Z is installed in Y on planker to limit switch; Probe unit comprises CCD observation parts and optical fiber detection part, is installed in Z on tooth bar; The optical fiber detection part itself has laser generator, receiver, light ray coupling device; Attemperating unit has ceramic heat-insulation board, ceramic heat-insulation board has the hole, device has the heating and cooling device on the ceramic heat-insulation board, the heating and cooling device can adopt semiconductor heating and cooling device, the heating and cooling device is equipped with heat shield or coating heat shield outward, between heating and cooling device and ceramic heat-insulation board, heat insulation is housed, is provided with little heat transfer sheet, be provided with thermal resistance sensor at little heat transfer sheet front end in heating and cooling device front.
2. according to the described a kind of PCR chip testing instrument with micro probe array of claim 1, it is characterized in that on the front panel of shell view window, power knob, start button, light source button, pneumatic button being arranged, light source and rear panel connecting groove are arranged on the rear panel.
3. according to the described a kind of PCR chip testing instrument with micro probe array of claim 1, the hardware controls circuit that it is characterized in that attemperating unit comprises that mainly extraction, proportional integral, proportion differential and the output amplification Drive and Control Circuit youngster of sensor signal partly form, the extraction circuit of sensor signal comprises power supply, resistance R 1 and R2, adjustable resistance R
W1, sensor resistance R
T, the proportion differential circuit comprises operational amplifier A 1, resistance R 4 and R3, adjustable resistance R
W2, the proportional integral circuit comprises operational amplifier A 2, resistance R 5, R6 and R7, adjustable resistance R
W3, amplification driving circuit comprises resistance R 8, diode D1 and D2, triode T1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99114456 CN1256415A (en) | 1999-09-23 | 1999-09-23 | Chip testing instrument with micro probe array |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99114456 CN1256415A (en) | 1999-09-23 | 1999-09-23 | Chip testing instrument with micro probe array |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1256415A true CN1256415A (en) | 2000-06-14 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 99114456 Pending CN1256415A (en) | 1999-09-23 | 1999-09-23 | Chip testing instrument with micro probe array |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005083432A1 (en) * | 2003-12-19 | 2005-09-09 | Chengdu Kuachang Medical Industrial Limited | The detecting method of chip and the detecting device |
| CN1297084C (en) * | 2005-01-24 | 2007-01-24 | 东南大学 | Microwave probe tester of integrated photodiode |
| CN1330963C (en) * | 2001-09-28 | 2007-08-08 | 株式会社日立制作所 | Luminescence detecting device, luminescence detecting mocroarray plate |
| CN100394183C (en) * | 2004-11-29 | 2008-06-11 | 开物科技股份有限公司 | Locating device and its locating method for biochip |
| CN101868721A (en) * | 2007-10-12 | 2010-10-20 | 比格科技私人有限公司 | Hand held micro PCR device |
| CN101589303B (en) * | 2007-01-17 | 2012-01-11 | 霍尼韦尔国际公司 | Microarray reader based on evanescent wave detection and method of reading a microarray |
| CN103801415A (en) * | 2014-03-12 | 2014-05-21 | 杭州霆科生物科技有限公司 | Button type microfluid control system and button type microfluid control method |
| CN104793095A (en) * | 2015-04-01 | 2015-07-22 | 广东韦达尔科技有限公司 | Silver paste line detector capable of filling gaps automatically |
| CN104792801A (en) * | 2015-04-01 | 2015-07-22 | 广东韦达尔科技有限公司 | Automatic detection machine for silver pulp line |
| CN104819986A (en) * | 2015-04-01 | 2015-08-05 | 广东韦达尔科技有限公司 | Panorama-type automatic repairing silver paste line detector |
| CN105400691A (en) * | 2015-12-11 | 2016-03-16 | 舟山医院 | A multifunctional PCR instrument |
| CN105929202A (en) * | 2016-04-25 | 2016-09-07 | 昆山瑞鸿诚自动化设备科技有限公司 | HOME key and front screen two-in-one test tool |
| WO2020224363A1 (en) * | 2019-05-06 | 2020-11-12 | 京东方科技集团股份有限公司 | Experimental apparatus for biochip |
| CN113281629A (en) * | 2021-04-10 | 2021-08-20 | 深圳市永而佳实业有限公司 | Light emitting diode array detection device and using method thereof |
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1999
- 1999-09-23 CN CN 99114456 patent/CN1256415A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1330963C (en) * | 2001-09-28 | 2007-08-08 | 株式会社日立制作所 | Luminescence detecting device, luminescence detecting mocroarray plate |
| WO2005083432A1 (en) * | 2003-12-19 | 2005-09-09 | Chengdu Kuachang Medical Industrial Limited | The detecting method of chip and the detecting device |
| CN100394183C (en) * | 2004-11-29 | 2008-06-11 | 开物科技股份有限公司 | Locating device and its locating method for biochip |
| CN1297084C (en) * | 2005-01-24 | 2007-01-24 | 东南大学 | Microwave probe tester of integrated photodiode |
| CN101589303B (en) * | 2007-01-17 | 2012-01-11 | 霍尼韦尔国际公司 | Microarray reader based on evanescent wave detection and method of reading a microarray |
| CN101868721A (en) * | 2007-10-12 | 2010-10-20 | 比格科技私人有限公司 | Hand held micro PCR device |
| CN101868721B (en) * | 2007-10-12 | 2012-12-19 | 比格科技私人有限公司 | Hand held micro PCR device |
| CN103801415A (en) * | 2014-03-12 | 2014-05-21 | 杭州霆科生物科技有限公司 | Button type microfluid control system and button type microfluid control method |
| CN104793095A (en) * | 2015-04-01 | 2015-07-22 | 广东韦达尔科技有限公司 | Silver paste line detector capable of filling gaps automatically |
| CN104792801A (en) * | 2015-04-01 | 2015-07-22 | 广东韦达尔科技有限公司 | Automatic detection machine for silver pulp line |
| CN104819986A (en) * | 2015-04-01 | 2015-08-05 | 广东韦达尔科技有限公司 | Panorama-type automatic repairing silver paste line detector |
| CN104819986B (en) * | 2015-04-01 | 2018-05-18 | 广东韦达尔科技有限公司 | A kind of silver paste line detection machine of panorama type auto-mending |
| CN104793095B (en) * | 2015-04-01 | 2018-10-30 | 广东韦达尔科技有限公司 | A kind of silver paste line detection machine that can be filled a vacancy automatically |
| CN105400691A (en) * | 2015-12-11 | 2016-03-16 | 舟山医院 | A multifunctional PCR instrument |
| CN105929202A (en) * | 2016-04-25 | 2016-09-07 | 昆山瑞鸿诚自动化设备科技有限公司 | HOME key and front screen two-in-one test tool |
| WO2020224363A1 (en) * | 2019-05-06 | 2020-11-12 | 京东方科技集团股份有限公司 | Experimental apparatus for biochip |
| US11993808B2 (en) | 2019-05-06 | 2024-05-28 | Beijing Boe Technology Development Co., Ltd. | Experimental apparatus for biochip |
| CN113281629A (en) * | 2021-04-10 | 2021-08-20 | 深圳市永而佳实业有限公司 | Light emitting diode array detection device and using method thereof |
| CN113281629B (en) * | 2021-04-10 | 2022-07-01 | 深圳市永而佳实业有限公司 | Light emitting diode array detection device and using method thereof |
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