CN111592982A - Novel portable nucleic acid amplification analyzer - Google Patents
Novel portable nucleic acid amplification analyzer Download PDFInfo
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- CN111592982A CN111592982A CN202010445551.4A CN202010445551A CN111592982A CN 111592982 A CN111592982 A CN 111592982A CN 202010445551 A CN202010445551 A CN 202010445551A CN 111592982 A CN111592982 A CN 111592982A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
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Abstract
The invention discloses a novel portable nucleic acid amplification analyzer, which comprises: hot cover part, display input module, epitheca, inferior valve, amplification module part, control module, side optical scanning module, signal processing analysis module, power module wherein: the amplification module part comprises a module cover, a metal reaction module, a light guide rod and a radiator, wherein the metal reaction module is provided with a detection hole for fluorescence detection; the amplification hole sites on the metal reaction modules are arranged in a staggered manner; the side optical scanning module comprises a Y-shaped optical transmission fiber, an optical filtering module, a left position optical coupler, a left shading sheet, a linear lead screw guide rail, a right shading sheet, a stepping motor and a left position optical coupler; the heat cover member includes: the metal back plate is heated by the MCH heating sheet clinging to the metal back plate; the display input module includes: the protective film, the touch screen, the liquid crystal screen and the display component core circuit board; the invention can greatly simplify the structure of the instrument, reduce the scanning detection time, reduce the volume of the instrument and reduce the overall cost of the instrument.
Description
Technical Field
The invention belongs to the field of molecular diagnosis, and relates to a novel portable nucleic acid amplification analyzer.
Background
The nucleic acid amplification detection technology is a process of providing in vitro amplification conditions for nucleic acid fragments, enabling the nucleic acid fragments to be amplified in an exponential manner in a large quantity, adding a fluorescent dye or a fluorescent marker in the nucleic acid amplification process, detecting the intensity of a fluorescent signal by adopting an optical device, and obtaining a nucleic acid amplification result by analyzing the fluorescent signal. Mainly comprises real-time fluorescence quantitative PCR (polymerase chain reaction) technology, constant temperature nucleic acid amplification detection technology and the like.
The optical device of the nucleic acid amplification analyzer in the prior art mainly adopts a fluorescence acquisition mode which comprises the following steps:
1. photographing all amplification reaction holes by adopting a photoelectric element (CCD) at the top of the amplification module part to obtain fluorescence data;
2. and scanning the amplification reaction wells one by using a photoelectric element (PMT) at the bottom of the amplification module component to acquire fluorescence data.
The detection mode of taking a picture of the whole amplification module component by adopting a photoelectric element (CCD) is not suitable for the development of portable equipment because the optical detection component needs a longer detection distance and is expensive. In addition, the method needs to realize the movement in two directions of an X axis and a Y axis, so that the movement scanning mechanism is complex, the equipment price is high, and the size is large.
Therefore, the existing nucleic acid amplification analyzers on the market generally have the defects of complex structure, high price and large volume.
With the development of isothermal nucleic acid technologies, such as Loop-mediated isothermal amplification (LAMP), Strand Displacement Amplification (SDA), Rolling Circle Amplification (RCA), etc., nucleic acid detection technologies gradually move out of laboratories, and the market demand for portable nucleic acid amplification analyzers increases day by day.
Disclosure of Invention
In order to solve the defects of the prior art, the invention provides a novel portable nucleic acid amplification analyzer which can greatly simplify the structure of the analyzer, reduce the volume of the analyzer and reduce the overall cost of the analyzer.
Novel portable nucleic acid amplification analyzer, characterized by including:
a housing including an upper shell and a lower shell;
the test tube hole sites of the amplification module component are distributed in a plurality of rows in a staggered and crossed manner;
the side optical scanning module is used for continuously exciting the test tube hole sites on the amplification module part and simultaneously scanning and reading fluorescence signals;
the signal processing and analyzing module is used for amplifying the fluorescence signal, analyzing and processing the fluorescence signal and shielding and filtering the environmental noise clutter;
the control component is used for controlling the movement of the movement mechanism, controlling the temperature of the reaction module, controlling the temperature of the hot cover and accessing signal data;
the display input module is used for displaying the fluorescence data and providing an input setting interface for a user;
a heat cover member for pressing the reaction cuvette to prevent evaporation of the reagent.
Preferably, the amplification module part comprises a module cover, a metal reaction module, a light guide rod and a heat sink.
Preferably, the side of the test tube hole site of the amplification module part is provided with a fluorescence detection hole.
Preferably, the amplification module assembly is used for temperature control of the reaction cuvette.
Preferably, the side optical scanning module comprises a Y-shaped optical transmission fiber, an optical filtering module, a linear motion mechanism, and a photoelectric positioning element.
Preferably, the display input module comprises a liquid crystal screen, a touch screen, a display component core circuit board and a protective film.
Preferably, the heat cover member includes a metal back plate, a heating sheet, a temperature measuring element, and a pressing device.
Compared with the prior art, the gain effect of the invention is as follows:
the fluorescence data of a plurality of rows of test tube reaction holes can be simultaneously acquired in the primary fluorescence scanning process;
when the nucleic acid amplification detection scheme with the same number of reaction holes is adopted, the volume is obviously small;
when the nucleic acid amplification detection scheme with the same number of reaction holes is adopted, the equipment cost is greatly reduced;
when the nucleic acid amplification detection scheme with the same number of reaction holes is adopted, the internal structure of the equipment is simpler and more stable;
when the nucleic acid amplification detection scheme with the same number of reaction holes is adopted, the detection scanning time is reduced by half;
the invention greatly optimizes the detection time, the equipment cost and the equipment volume, thereby expanding the application range of the detection equipment.
Drawings
FIG. 1 is a perspective view showing the overall structure of a novel portable nucleic acid amplification analyzer.
FIG. 2 is a three-dimensional view of the overall structure of the novel portable nucleic acid amplification analyzer.
FIG. 3 is a three-dimensional view of the reaction module components of the novel portable nucleic acid amplification analyzer.
FIG. 4 is a perspective view of the reaction module components of the novel portable nucleic acid amplification analyzer.
FIG. 5 is a perspective view of a side optical scanning unit of the novel portable nucleic acid amplification analyzer.
FIG. 6 is a side cross-sectional view of a heat cover part of the novel portable nucleic acid amplification analyzer.
FIG. 7 is a perspective view of a display input module of the novel portable nucleic acid amplification analyzer.
FIG. 8 is a schematic diagram of a signal processing and analyzing module of the novel portable nucleic acid amplification analyzer.
FIG. 9 is a schematic diagram of the overall control of the portable nucleic acid amplification analyzer.
Detailed Description
The technical embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings.
As shown in FIGS. 1 and 2, the present invention discloses a novel portable nucleic acid amplification analyzer, comprising: hot lid part (1), show input module (2), epitheca (3), inferior valve (4), amplification module part (5), control module (7), side optical scanning module (8), signal processing analysis module (9), power module (10), wherein:
an amplification module part (5) as shown in fig. 3, which comprises a module cover (11), a metal reaction module (12), a light guide rod (14) and a radiator (15), wherein the metal reaction module (12) is provided with a monitoring hole (13) for fluorescence detection; the amplification hole sites on the metal reaction module (12) are arranged in a double-row staggered manner, and the arrangement mode can realize that fluorescence data in the double-row reaction holes can be read simultaneously by one-time scanning; the light guide rod (14) is arranged in the detection hole and used for conducting the fluorescence signal, and the distance loss of the fluorescence signal is reduced.
Side optical scanning module (8), as shown in fig. 5 contain Y type optical transmission fiber (17), optical filtering module (18), left side position opto-coupler (19), left side anti-dazzling screen (20), linear screw guide rail (21), right side anti-dazzling screen (22), step motor (23), left side position opto-coupler (24), left side anti-dazzling screen (20) right side anti-dazzling screen (22) set up the left and right sides in optical filtering module (18), the optical scanning in-process, step motor (23) drive linear screw guide rail rotates, thereby drive Y type optical transmission fiber (17), optical filtering module (18) side-to-side movement, return when moving about to opto-coupler position.
As shown in fig. 6, the heat cover member (1) includes: the metal back plate (25), the MCH heating plate (26), the thermistor (27), the spring pressing device (28) and the hot cover shell (29), wherein the thermistor (27) is arranged inside the metal back plate (25) and used for detecting the real-time temperature of the metal back plate (25), and the MCH heating plate (26) is tightly attached to the metal back plate (25) to heat the metal back plate (25).
The display input module (2), as shown in fig. 7, includes: protective film (30), touch-sensitive screen (31), LCD screen (32), display element core circuit board (33), protective film (30) are arranged in the protection experiment in-process liquid intrusion instrument, and touch-sensitive screen (31) are used for receiving the operation of experimenter, and LCD screen (32) are used for showing experimental information.
The signal processing and analyzing module (9) has the principle as shown in fig. 8: the CPU controls the LED to emit an excitation light source, irradiates the reaction test tube, emits a fluorescence signal, converts the fluorescence signal into an electric signal through the silicon photodiode, amplifies the weak electric signal by millions of times through the analog amplification circuit, converts the weak electric signal into a digital signal through A/D (analog/digital) and finally performs analysis processing through the CPU.
The control flow of the novel portable nucleic acid amplification analyzer is described in detail below with reference to FIG. 9.
The novel portable nucleic acid amplification analyzer is started, the power module (10) supplies power to the whole analyzer, an experimenter inputs an experiment program through the display input module (2), the control module (7) controls the temperature of the amplification module (5) according to the experiment program, the temperature of the hot cover component (1) is controlled, the side optical scanning module (8) is controlled to perform linear motion to acquire fluorescence data, and the signal processing and analyzing module (9) is controlled to perform processing and analyzing on the fluorescence data. The heat cover part (1) heats the top of the reaction test tube to prevent the reagent from evaporating, and the amplification module (5) controls the temperature of the reaction test tube to realize the nucleic acid amplification reaction. And the final control module (7) displays the experimental data through the display input module (2).
Although the present invention has been described in detail, the present invention is only for the purpose of illustration and not limitation, and any invention that does not depart from the spirit of the present invention falls within the scope of the present invention.
Claims (7)
1. Novel portable nucleic acid amplification analyzer, characterized by including:
a housing including an upper shell and a lower shell;
the test tube hole sites of the amplification module component are distributed in a plurality of rows in a staggered and crossed manner;
the side optical scanning module is used for continuously exciting the test tube hole sites on the amplification module part and simultaneously scanning and reading fluorescence signals;
the signal processing and analyzing module is used for amplifying the fluorescence signal, analyzing and processing the fluorescence signal and shielding and filtering the environmental noise clutter;
the control module is used for controlling the movement of the movement mechanism, controlling the temperature of the reaction module, controlling the temperature of the hot cover and accessing signal data;
the display input module is used for displaying the fluorescence data and providing an input setting interface for a user;
a heat cover member for pressing the reaction cuvette to prevent evaporation of the reagent;
and the power supply driving module is used for providing a required power supply for the whole machine.
2. The portable nucleic acid amplification analyzer of claim 1, wherein the amplification module unit comprises a module housing, a metal reaction module, a light guide rod, and a heat sink.
3. The portable nucleic acid amplification analyzer of claim 1, wherein the amplification module unit has a fluorescence detection hole formed in a side surface of the test tube hole.
4. The portable nucleic acid amplification analyzer of claim 1, wherein the amplification module unit is used for temperature control of the reaction cuvette.
5. The portable nucleic acid amplification analyzer of claim 1, wherein the lateral optical scanning module comprises a Y-shaped optical transmission fiber, an optical filter module, a linear motion mechanism, and a photoelectric positioning element.
6. The portable nucleic acid amplification analyzer of claim 1, wherein the display input module comprises a liquid crystal panel, a touch panel, a display unit core circuit board, and a protective film.
7. The portable nucleic acid amplification analyzer of claim 1, wherein the heat-sealing member comprises a metal back plate, a heating plate, a temperature measuring element, and a pressing device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010445551.4A CN111592982A (en) | 2020-05-24 | 2020-05-24 | Novel portable nucleic acid amplification analyzer |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010445551.4A CN111592982A (en) | 2020-05-24 | 2020-05-24 | Novel portable nucleic acid amplification analyzer |
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| Publication Number | Publication Date |
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| CN111592982A true CN111592982A (en) | 2020-08-28 |
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| CN202010445551.4A Pending CN111592982A (en) | 2020-05-24 | 2020-05-24 | Novel portable nucleic acid amplification analyzer |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113049564A (en) * | 2021-03-26 | 2021-06-29 | 江苏宏微特斯医药科技有限公司 | Fluorescence detection device |
Citations (6)
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| CN202741179U (en) * | 2012-08-10 | 2013-02-20 | 深圳德夏科技发展有限公司 | Sample rack |
| CN204589191U (en) * | 2014-09-25 | 2015-08-26 | 江南大学 | Real time fluorescent quantitative constant temperature nucleic acid amplification instrument |
| CN105092543A (en) * | 2014-05-12 | 2015-11-25 | 绍兴安尼特微电子科技有限公司 | Portable fluorescence quantitative PCR detector |
| CN107043697A (en) * | 2017-06-14 | 2017-08-15 | 北京百康芯生物科技有限公司 | A kind of multi-channel chip constant-temperature amplification detection of nucleic acids instrument |
| CN207832675U (en) * | 2018-02-11 | 2018-09-07 | 金博特（北京）生物科技有限公司 | A kind of tubular type constant temperature nucleic acid amplification detector |
| CN210287354U (en) * | 2018-12-26 | 2020-04-10 | 中国检验检疫科学研究院 | Real-time fluorescence amplification detector |
-
2020
- 2020-05-24 CN CN202010445551.4A patent/CN111592982A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202741179U (en) * | 2012-08-10 | 2013-02-20 | 深圳德夏科技发展有限公司 | Sample rack |
| CN105092543A (en) * | 2014-05-12 | 2015-11-25 | 绍兴安尼特微电子科技有限公司 | Portable fluorescence quantitative PCR detector |
| CN204589191U (en) * | 2014-09-25 | 2015-08-26 | 江南大学 | Real time fluorescent quantitative constant temperature nucleic acid amplification instrument |
| CN107043697A (en) * | 2017-06-14 | 2017-08-15 | 北京百康芯生物科技有限公司 | A kind of multi-channel chip constant-temperature amplification detection of nucleic acids instrument |
| CN207832675U (en) * | 2018-02-11 | 2018-09-07 | 金博特（北京）生物科技有限公司 | A kind of tubular type constant temperature nucleic acid amplification detector |
| CN210287354U (en) * | 2018-12-26 | 2020-04-10 | 中国检验检疫科学研究院 | Real-time fluorescence amplification detector |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113049564A (en) * | 2021-03-26 | 2021-06-29 | 江苏宏微特斯医药科技有限公司 | Fluorescence detection device |
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