CN210604385U - Rapid pesticide residue detection system based on flower-shaped nano silver substrate - Google Patents
Rapid pesticide residue detection system based on flower-shaped nano silver substrate Download PDFInfo
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- CN210604385U CN210604385U CN201921288787.0U CN201921288787U CN210604385U CN 210604385 U CN210604385 U CN 210604385U CN 201921288787 U CN201921288787 U CN 201921288787U CN 210604385 U CN210604385 U CN 210604385U
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Abstract
The utility model discloses a pesticide residue short-term test system based on flower form nanometer silver substrate, include: the microfluidic disc comprises a microfluidic disc centrifugal platform, a fluorescence detection device and a terminal processing device; the microfluidic disc centrifugal platform comprises a first single chip microcomputer, a lab-on-a-chip with a disc array structure and a brushless motor; flower-shaped nano silver particles are arranged in a lab-on-a-chip of the disc array structure; the lab-on-a-chip of the disc array structure is arranged on the brushless motor; the first single chip microcomputer is in control connection with the brushless motor; the fluorescence detection device is used for acquiring a light intensity signal of the pesticide to be detected in a lab-on-a-chip with the disc array structure; and the terminal processing device is connected with the fluorescence detection device, collects the light intensity signal of the pesticide to be detected, and detects pesticide residue. The system can realize the functions of pretreatment-free and rapid qualitative and quantitative analysis of the sample to be detected, and improves the reliability of the detection result.
Description
Technical Field
The utility model belongs to pesticide residue detection area especially relates to a pesticide residue short-term test system based on flower form nanometer silver substrate.
Background
Pesticide residue and pollution have become the important problem that influences agricultural sustainable development, in order to control pesticide residue better, protect food safety and ecological environment, need to develop swift, accurate, high-efficient, low-cost pesticide residue detection device urgently. At present, the detection method for detecting pesticide residues by using the traditional spectrum specified by the national standard generally has the defects of complicated measurement process, expensive instrument, technical requirements on operators, higher cost, inconvenience for large-area popularization and use and the like, and generally cannot meet the requirements of rapid detection and on-site detection. The gas chromatography-mass spectrometry (GC-MS) combined method for detecting the sample has the advantages of high-sensitivity qualitative and quantitative detection, but the instrument is expensive and the technical operation requirement is high; the detection of pesticide residue by a liquid chromatography-mass spectrometry (LC-MS) method can realize the detection of ppb level of the pesticide residue, but the sample pretreatment process is complex and the field operation cannot be realized. Researches show that the surface enhanced fluorescence spectroscopy analysis method for preparing the metal nano-structure substrate with various microscopic appearances realizes effective regulation and control of optical signals of probe molecules, improves the sensitivity and signal-to-noise ratio of detection signals, and can be used for solving the problems faced by the traditional spectroscopy technology. In recent years, the microfluidic technology is mature continuously, and a large amount of documents can be referred for reference; in addition, the test equipment such as ultrasonic, magnetic stirring and vacuum drying boxes and the detection technology such as a fluorescence spectrometer, a fluorescence analyzer and a fluorescence imager are quite mature.
However, at present, a device capable of effectively and rapidly quantifying pesticide residues is still lacking.
SUMMERY OF THE UTILITY MODEL
In order to overcome not enough among the prior art, the utility model provides a pesticide residue short-term test system based on flower form nanometer silver substrate, its aim at can carry out quick ration to pesticide residue effectively, can carry out non-interfering transmission to fluorescence signal again, improves and detects the precision.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
the utility model provides a pesticide residue short-term test system based on flower form nanometer silver substrate, include: the microfluidic disc comprises a microfluidic disc centrifugal platform, a fluorescence detection device and a terminal processing device;
the microfluidic disc centrifugal platform comprises a first single chip microcomputer, a lab-on-a-chip with a disc array structure and a brushless motor; flower-shaped nano silver particles are arranged in a lab-on-a-chip of the disc array structure; the lab-on-a-chip of the disc array structure is arranged on the brushless motor; the first single chip microcomputer is in control connection with the brushless motor;
the fluorescence detection device is used for acquiring a light intensity signal of the pesticide to be detected in a lab-on-a-chip with the disc array structure;
and the terminal processing device is connected with the fluorescence detection device, collects the light intensity signal of the pesticide to be detected, and detects pesticide residue.
In one embodiment, the fluorescence detection device comprises: the device comprises an optical fiber panel, a PIN array chip (202), an ADL5315 chip, an AD7190 module and a second single chip microcomputer;
the optical fiber panel is positioned above the PIN array chip and is used for converging the fluorescent signals and irradiating the fluorescent signals onto the PIN array chip;
the ADL5315 chip is connected with the PIN array chip and is positioned below the PIN array chip;
one end of the AD7190 module is connected with the ADL5315 chip, and the other end of the AD7190 module is connected with the second single chip microcomputer.
In one embodiment, the terminal processing device includes: the USB signal acquisition board card and the upper computer;
one end of the USB signal acquisition board card is connected with the second single chip microcomputer, and the other end of the USB signal acquisition board card is connected with the upper computer.
In one embodiment, the microfluidic disk centrifugation platform further comprises: a display screen;
the first single chip microcomputer is connected with the display screen and controls the display screen to display working state parameters of the brushless motor.
In one embodiment, the display screen is a serial port HMI liquid crystal screen.
In one embodiment, the lab-on-a-chip of the disk array structure includes: the device comprises a fixed mounting hole, a dropping port, a reaction tank and a detection tank;
the fixed mounting hole is arranged on a driving output shaft of the brushless motor;
the drip inlet is communicated with the reaction pool and the detection pool sequentially through a microfluidic channel;
flower-shaped nano silver particles are placed in the detection pool.
In one embodiment, the lab-on-a-chip of the disk array structure is one or more of:
y-type array chip laboratory, multi-index detection chip laboratory and sample mixed chip laboratory.
In one embodiment, the first singlechip is an STM32 singlechip.
In one embodiment, the second singlechip is an STM32 singlechip.
In one embodiment, the system further comprises: a laser source; the laser emitted by the laser source covers the detection cell.
The utility model provides a pesticide residue short-term test system based on flower form nanometer silver substrate has integrateed micro-fluidic disc centrifugal platform, fluorescence detection device and terminal processing apparatus three, can realize exempting from preliminary treatment, the function of qualitative quantitative analysis fast to the sample that awaits measuring, has improved the reliability of testing result.
Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and claims hereof as well as the appended drawings
The technical solution of the present invention is further described in detail by the accompanying drawings and examples.
Drawings
The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention and not to limit the invention. In the drawings:
fig. 1 is a block diagram of a rapid pesticide residue detection system based on a flower-shaped nano silver substrate according to an embodiment of the present invention;
fig. 2 is a structural diagram of a rapid pesticide residue detection system based on a flower-shaped nano silver substrate according to an embodiment of the present invention;
fig. 3 is a plan view of a lab-on-a-chip with a disk array structure according to an embodiment of the present invention;
FIG. 4 is a plan view of a Y-type array lab-on-a-chip;
FIG. 5 is a plan view of a multi-index lab-on-a-chip;
FIG. 6 is a plan view of a sample hybrid-chip lab.
In the drawings:
1-microfluidic disc centrifugal platform; 2-a fluorescence detection device; 3-is a terminal processing device; 101-a first singlechip; 102-a display screen; 103-lab-on-a-chip in a disk array configuration; 104-a brushless motor; 201-fiber optic faceplate; 202-PIN array chip; 203-ADL5315 chip; a 204-AD7190 module; 205-a second single chip microcomputer; 301-USB signal acquisition board card; 302-an upper computer; 1031-fixing mounting holes; 1032-drip inlet; 1033-a reaction cell; 1034-detection pool.
Detailed Description
Exemplary embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings. While exemplary embodiments of the present disclosure are shown in the drawings, it should be understood that the present disclosure may be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art.
The utility model provides a pair of pesticide residue short-term test system based on flower form nanometer silver substrate, it is shown with reference to fig. 1-2, include: the device comprises a microfluidic disc centrifugal platform 1, a fluorescence detection device 2 and a terminal processing device 3;
wherein, the microfluidic disc centrifugal platform 1 is provided with a first singlechip 101, a lab-on-a-chip 103 with a disc array structure and a brushless motor 104; flower-shaped nano silver particles are arranged in the lab-on-a-chip 103 with the disc array structure; the flower-shaped nano-silver SEF substrate has high sensitivity, effectively improves the fluorescence spectrum of the substance, and improves the accuracy of the experiment.
As shown in fig. 1, a lab-on-a-chip 103 of a disc array structure is mounted on a brushless motor 104, and a first single chip microcomputer 101 is used to control the operating states of the brushless motor 104, such as on, rotation direction, rotation speed, and off.
The fluorescence detection device 2 is used for acquiring a light intensity signal of pesticide to be detected in the lab-on-a-chip 103 with a disc array structure; the light intensity signal is sent to a terminal processing device for detection and analysis, so that the pesticide residue can be detected qualitatively and quantitatively; the system can effectively improve the reliability of the detection result.
Further, referring to fig. 1, the fluorescence detection device 2 includes: the system comprises an optical fiber panel 201, a PIN array chip 202, an ADL5315 chip 203, an AD7190 module 204 and a second singlechip 205; the optical fiber panel 201 is located above the PIN array chip 202, and the optical fiber panel 201 converges the fluorescent signal and irradiates the PIN array chip 202.
The ADL5315 chip 203 is located below the PIN array chip 202, and the ADL5315 chip 203 amplifies and filters current signals acquired by the PIN array chip 202; and then the analog voltage signal is acquired by an AD7190 module 204, and finally the analog voltage signal is converted into a digital signal which can be acquired by the terminal processing system 3 through a second single chip microcomputer 205.
Further, the terminal processing system 3 includes a USB signal acquisition board 301 and an upper computer 302. The USB signal acquisition board 301 uploads the digital signal of the second single chip 205 to the upper computer 302. The upper computer can quickly, qualitatively and quantitatively detect the pesticide residue by adopting algorithms such as an SVM (support vector machine), a BP (back propagation) neural network and the like.
Further, the microfluidic disk centrifugation platform 1 may further include: the display screen 102 is connected with the first singlechip 101 and can be used for displaying working state parameters of the brushless motor 104; such as displaying the operating status of the brushless motor and controlling the operating instructions of the brushless motor, such as the rotating direction and rotating speed. The display screen can select a serial port HMI liquid crystal screen, manual interaction operation can be realized, and control instructions can be conveniently input. The first single chip microcomputer and the second single chip microcomputer can both select the same STM32 series single chip microcomputer, and the embodiment of the disclosure does not limit the same.
Further, referring to fig. 3, the lab-on-a-chip 103 of the disk array structure includes: a fixed mounting hole 1031, a dropping port 1032, a reaction cell 1033, and a detection cell 1034;
wherein: a fixing mounting hole 1031 is provided in the middle of the lab-on-a-chip for mounting on a driving output shaft of the brushless motor 104; can rotate along with the output shaft;
the dripping port 1032 and the reaction cell 1033, and the reaction cell 1033 and the detection cell 1034 are communicated through a microfluidic channel; flower-like nano silver particles are placed in the detection cell 1034.
In addition, a plurality of dropping ports 1032, reaction cells 1033, and detection cells 1034 may be disposed on the lab-on-a-chip 103 of the disk array structure. Referring to fig. 4-6, the shape arrangement may be a Y-array lab-on-a-chip, a multi-index lab-on-a-chip, or a sample-mixing lab-on-a-chip. The embodiments of the present disclosure do not limit the specific type and shape of lab-on-a-chip of the disk array structure.
Further, when the system works, the needed laser source can be provided by a third-party laser source; of course, a laser source may also be included; for example, the laser source can be arranged on a fluorescence detection device, as long as the emitted laser covers the detection cell, and the position of the specific laser source is not limited.
The rapid pesticide residue detection system based on the flower-shaped nano silver substrate provided by the embodiment has the following working principle: according to the structural connection shown in fig. 1-2, flower-like nano silver is pre-embedded in a detection pool 1034 in a chip laboratory 103 with a disc array structure, after a laser light source is emitted, an STM32 single chip microcomputer (a first single chip microcomputer) controls a motor driver to drive a direct current brushless motor, a liquid crystal screen of a serial port HMI user displays the working state of a brushless motor 104 and controls the working characteristics such as the rotating direction, the rotating speed and the like of the brushless motor 104, pesticide residues enter the chip laboratory 104 with the disc array structure through a micro-flow channel in a centrifugal mode, pesticide molecules and enzyme are mixed in a reaction pool of the chip laboratory 103 with the disc array structure under the action of centrifugal force, enter the reaction channel and are fully mixed and reacted, then enter the detection pool, the spectrum radiation intensity of fluorescent species close to a flower-like nano silver substrate is increased compared with the intensity in a free state, the detection pool is irradiated, the generated fluorescent signal is collected by the optical fiber panel 201, the PIN array chip 202 converts the received optical signal from the optical fiber panel 201 into a current signal, the weak current signal generated by the PIN array chip 202 is amplified and filtered by the ADL5315 chip 203 and then is collected by the AD7190 module 204, the STM32 fits the voltage signal to obtain a light intensity signal, the light intensity signal is collected by the USB signal collection board 301, the digital signal is automatically output to the PC end by the USB signal collection board 301, and the upper computer can adopt algorithms such as an SVM (support vector machine), a BP (back propagation) neural network and the like, so that the pesticide residue can be quickly, qualitatively and quantitatively detected.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. A pesticide residue rapid detection system based on flower-shaped nano silver substrate is characterized in that: the method comprises the following steps: the device comprises a microfluidic disc centrifugal platform (1), a fluorescence detection device (2) and a terminal processing device (3);
the microfluidic disc centrifugal platform (1) comprises a first single chip microcomputer (101), a lab-on-a-chip (103) with a disc array structure and a brushless motor (104); flower-shaped nano silver particles are arranged in a lab-on-a-chip (103) of the disc array structure; the lab-on-a-chip (103) of the disc array structure is arranged on the brushless motor (104); the first single chip microcomputer (101) is in control connection with the brushless motor (104);
the fluorescence detection device (2) is used for acquiring a light intensity signal of pesticide to be detected in a lab-on-a-chip (103) with the disc array structure;
and the terminal processing device (3) is connected with the fluorescence detection device (2), collects the light intensity signal of the pesticide to be detected, and detects pesticide residues.
2. The rapid pesticide residue detection system based on the flower-shaped nano silver substrate as claimed in claim 1, characterized in that: the fluorescence detection device (2) comprises: the system comprises an optical fiber panel (201), a PIN array chip (202), an ADL5315 chip (203), an AD7190 module (204) and a second single chip microcomputer (205);
the optical fiber panel (201) is located above the PIN array chip (202), and the fluorescent signals are converged and then irradiated onto the PIN array chip (202) by the optical fiber panel (201);
the ADL5315 chip (203) is connected with the PIN array chip (202) and is positioned below the PIN array chip (202);
one end of the AD7190 module (204) is connected with the ADL5315 chip (203), and the other end of the AD7190 module is connected with the second singlechip (205).
3. The rapid pesticide residue detection system based on the flower-shaped nano silver substrate as claimed in claim 2, characterized in that: the terminal processing device (3) includes: the USB signal acquisition board card (301) and the upper computer (302);
one end of the USB signal acquisition board card (301) is connected with the second single chip microcomputer (205), and the other end of the USB signal acquisition board card is connected with the upper computer (302).
4. The rapid pesticide residue detection system based on the flower-shaped nano silver substrate as claimed in claim 1, characterized in that: the microfluidic disc centrifugation platform (1) further comprises: a display screen (102);
the first single chip microcomputer (101) is connected with the display screen (102) and controls the display screen (102) to display working state parameters of the brushless motor (104).
5. The rapid pesticide residue detection system based on the flower-shaped nano silver substrate as claimed in claim 4, wherein: the display screen (102) is a serial port HMI liquid crystal screen.
6. The rapid pesticide residue detection system based on the flower-shaped nano silver substrate as claimed in claim 1, characterized in that: the lab-on-a-chip (103) of a disk array structure includes: a fixed mounting hole (1031), a dropping port (1032), a reaction cell (1033) and a detection cell (1034);
the fixed mounting hole (1031) is mounted on a drive output shaft of the brushless motor (104);
the drip inlet (1032) is communicated with the reaction cell (1033) and the detection cell (1034) through a microfluidic channel in sequence;
flower-shaped nano silver particles are placed in the detection pool (1034).
7. The rapid pesticide residue detection system based on the flower-shaped nano silver substrate as claimed in claim 6, wherein: the lab-on-a-chip (103) of the disk array structure is one or more of:
y-type array chip laboratory, multi-index detection chip laboratory and sample mixed chip laboratory.
8. The rapid pesticide residue detection system based on the flower-shaped nano silver substrate as claimed in claim 1, characterized in that: the first single chip microcomputer (101) is an STM32 single chip microcomputer.
9. The rapid pesticide residue detection system based on the flower-shaped nano silver substrate as claimed in claim 2, characterized in that: the second singlechip (205) is an STM32 singlechip.
10. The rapid pesticide residue detection system based on the flower-shaped nano silver substrate as claimed in claim 6, wherein: the system further comprises: a laser source; the laser emitted by the laser source covers the detection cell.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111579766A (en) * | 2020-05-25 | 2020-08-25 | 成都微康生物科技有限公司 | Disc-type microfluidic fluorescence immunoassay analyzer and immunoassay item testing method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111579766A (en) * | 2020-05-25 | 2020-08-25 | 成都微康生物科技有限公司 | Disc-type microfluidic fluorescence immunoassay analyzer and immunoassay item testing method |
| CN111579766B (en) * | 2020-05-25 | 2021-06-11 | 成都微康生物科技有限公司 | Disc-type microfluidic fluorescence immunoassay analyzer and immunoassay item testing method |
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