CN211713091U - Non-contact portable miniature adjustable automatic cell counting equipment - Google Patents

Non-contact portable miniature adjustable automatic cell counting equipment Download PDF

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Publication number
CN211713091U
CN211713091U CN201921611244.8U CN201921611244U CN211713091U CN 211713091 U CN211713091 U CN 211713091U CN 201921611244 U CN201921611244 U CN 201921611244U CN 211713091 U CN211713091 U CN 211713091U
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China
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cell
cell counting
induction
shell
counting device
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CN201921611244.8U
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Inventor
李永贤
张顺聪
梁德
唐永超
郭惠智
郭丹青
罗培杰
周腾鹏
马延怀
刘攀杰
莫国业
李婧斓
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First Affiliated Hospital of Guangzhou University of Chinese Medicine
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First Affiliated Hospital of Guangzhou University of Chinese Medicine
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Abstract

The utility model discloses a non-contact portable micro-adjustable automatic cell counting device, which relates to the technical field of life science, and comprises a shell, wherein the top end of the shell is fixed with a mounting seat, and one side of the mounting seat, which is positioned at the top part of the shell, is provided with an auxiliary connecting plate in an arc structure, the inside of the mounting seat is inserted with a connecting rod, the top end of the connecting rod is fixed with a button, and a section of the connecting rod, which is positioned inside the mounting seat, is externally sheathed with a spring, the utility model utilizes the commonality of cells to have cell nucleuses, and adopts the conditions of scanning cells in each cell hole in a cell plate, such as absorption gray value, infrared limited regression technology and the like, based on the Kulter principle, thereby realizing that the cell amount in each cell hole in the cell plate can be known at any time, being convenient for the intervention and further operation of experiments and the like, saves time and labor and greatly improves the cell counting efficiency.

Description

Non-contact portable miniature adjustable automatic cell counting equipment
Technical Field
The utility model belongs to the technical field of the life science, concretely relates to automatic cell counting equipment of portable miniature controllability of non-contact.
Background
Cell counting is in wide demand in the field of life science research and in the medical field, and as a commonly used detection index, the number of cells per unit volume in a specific cell sample is very important. The current main cell counting method is a method based on microscopic images under the principles of electrical impedance method and optical amplification, wherein the electrical impedance method is based on the Coulter principle, and the counting is realized by changing the impedance caused by a cell passing through a small hole so as to cause the change of voltage or current signals; microscopic image-based methods under the principle of optical magnification rely on manual counting under a human eye microscope, such as cell counting plates.
Counting is generally performed by using a cell counting plate under a microscope during cell experiments, but the prior art has the following disadvantages during use: (1) the accuracy is difficult to achieve; (2) the time consumption is long; (3) high concentration of the operator is required, otherwise errors are easily generated; (4) only a small number of cells can be calculated, and a large number of cells can be counted only in a conversion mode; (5) easily causing cell pollution and cell waste.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide an automatic cell counting equipment of portable miniature controllability of non-contact to solve the problem that proposes in the above-mentioned background art, can avoid the pollution and the waste of cell, realize easy and simple to handle, labour saving and time saving reaches more accurate, the efficient purpose of cell count.
In order to achieve the above object, the utility model provides a following technical scheme: a non-contact portable micro-adjustable automatic cell counting device comprises a shell, wherein a mounting seat is fixed at the top end of the shell, an auxiliary connecting plate in an arc-shaped structure is installed at one side, located at the top of the mounting seat, of the top of the shell, a connecting rod is inserted into the mounting seat, a button is fixed at the top end of the connecting rod, a spring is sleeved outside one section, located inside the mounting seat, of the connecting rod, a display screen is embedded on the upper surface of the auxiliary connecting plate, a built-in cell nucleus sensing system is arranged inside the shell, a sensing end is installed at the bottom end of the shell, a light-gathering cover is clamped at the bottom end of the sensing end, the built-in cell nucleus sensing system comprises an image processing module, a cell sensing module, a main control chip, a data storage module and a power supply device, the image processing module is installed inside the auxiliary connecting plate, the cell sensing module is, the main control chip, the data storage module and the power supply device are respectively arranged in the shell.
Preferably, the shell is of a middle cylindrical structure, and the front surface of the shell is bonded with an anti-slip sleeve matched with the shell in shape.
Preferably, the image processing module adopts an image sensor, and is used for generating an image from the cell data sensed by the cell sensing module and displaying the image through a display screen, so as to facilitate cell counting.
Preferably, the cell induction module adopts an infrared sensor, the infrared sensor emits infrared rays, a ray outlet is formed in the bottom end of the light-gathering cover, and the infrared rays penetrate through the ray outlet and irradiate the cells in the cell hole so as to capture the cells.
Preferably, the power supply device adopts a rechargeable low-power-consumption battery for supplying power to the equipment.
Preferably, the data storage module adopts a memory card for storing data information of cell count.
Preferably, one side of the shell, which is opposite to the anti-skid sleeve, is provided with a placing groove and a rotating groove, wherein the rotating groove is positioned below the placing groove, the rotating groove is positioned at the bottom end of the shell, and the top of the induction end is rotatably connected with the rotating groove through a damping rotating shaft.
Preferably, the shape of standing groove and the shape phase-match of response end and snoot, and two arcs have been seted up to the bilateral symmetry of the first half section of standing groove to be used for digging up response end and snoot, be convenient for the rotation of response end and snoot is overlapped.
Preferably, the opposite corners of the top of the sensing end are all transited by arc chamfers to form smooth arc parts of the surface wall, so that the rotation of the sensing end is facilitated.
Preferably, the middle positions of the two sides of the inner wall of the placing groove are symmetrically provided with arc parts matched with the connecting positions of the induction end and the light-gathering cover, so that the induction end and the light-gathering cover can be conveniently placed in the placing groove.
Compared with the prior art, the beneficial effects of the utility model are that:
(1) the utility model discloses utilize the commonality of cell to have the cell nucleus, based on the coulter principle, adopt for example to absorb the condition of every downthehole cell of cell in the scanning cell board such as grey level value, the limited regression technique of infrared ray to the cell volume in every cell hole in the cell board can be known at any time to the realization, so that the intervention and the further operation of experiment etc. labour saving and time saving has improved the efficiency of cell count widely.
(2) The utility model discloses can the light operation of one hand, and portable can use in a flexible way in the laboratory, and it is convenient to adjust.
(3) Scanning the cells using, for example, absorption gray scale values, infrared finite regression techniques, etc., enables non-contact of the device, thereby avoiding contamination and waste of the cells.
(4) Dead cells can be eliminated, and the influence of the dead cells on the calculation of the number of the living cells is effectively avoided, so that the cell counting is more accurate.
Drawings
Fig. 1 is a perspective view of the present invention;
fig. 2 is a side view of the present invention;
fig. 3 is a front view of the present invention;
FIG. 4 is a schematic view of the internal structure of the housing of the present invention
FIG. 5 is a block diagram of the built-in cell nucleus induction system of the present invention;
fig. 6 is a rear view of the present invention;
fig. 7 is a rear view of the utility model when the light-gathering cover and the induction end are folded;
FIG. 8 is an enlarged view taken at A in FIG. 6;
FIG. 9 is an enlarged view of FIG. 7 at B;
FIG. 10 is an enlarged view at C of FIG. 7;
in the figure: 1-a shell; 1001-built-in nucleus sensing system; 2-auxiliary connecting plate; 3-a display screen; 4-a button; 5-mounting a base; 6-anti-slip sleeve; 7-a light-gathering cover; 8-a sensing end; 9-a connecting rod; 10-a rotating groove; 11-placing a groove; 12-an arc-shaped groove; 13-damping rotating shaft; 14-an arc portion; 15-an image processing module; 16-a cell sensing module; 17-a master control chip; 18-a data storage module; 19-power supply means.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1 to 5, embodiment 1: a non-contact portable micro-adjustable automatic cell counting device comprises a shell 1, wherein a mounting seat 5 is fixed at the top end of the shell 1, an auxiliary connecting plate 2 in an arc-shaped structure is installed on one side, located at the top of the mounting seat 5, of the top of the shell 1, a connecting rod 9 is inserted into the mounting seat 5, a button 4 is fixed at the top end of the connecting rod 9, a spring is sleeved outside one section, located inside the mounting seat 5, of the connecting rod 9, a display screen 3 is embedded on the upper surface of the auxiliary connecting plate 2, a built-in cell nucleus induction system 1001 is arranged inside the shell 1, an induction end 8 is installed at the bottom end of the shell 1, a light-gathering cover 7 is clamped at the bottom end of the induction end 8, the built-in cell nucleus induction system 1001 comprises an image processing module 15, a cell induction module 16, a main control chip 17, a data storage module 18 and a power supply device 19, wherein, the cell sensing module 16 is installed inside the sensing terminal 8, the main control chip 17, the data storage module 18 and the power supply device 19 are respectively installed inside the housing 1, during the experiment, the main control chip 17 controls the cell induction module 16 to work, the cell induction module 16 emits infrared rays, the infrared rays penetrate through the ray outlet of the light-gathering shade 7 and irradiate the cells in the cell holes on the cell plate, then the main control chip 17 sends the cell data sensed by the cell sensing module 16 to the image processing module 15, the image processing module 15 processes the data to generate an image, which is displayed by the display screen 3, by scanning cells in each well of the cell plate based on the coulter principle using, for example, an absorption gray scale value, an infrared ray finite regression technique, or the like, therefore, the cell quantity in each cell hole in the cell plate can be known at any time, and the cell counting efficiency is greatly improved.
Furthermore, the shell 1 is of a middle cylindrical structure, the front surface of the shell 1 is bonded with an anti-slip sleeve 6 matched with the shape of the shell, and the anti-slip sleeve 6 is made of rubber and plays a role of anti-slip.
Specifically, the image processing module 15 employs an image sensor for generating an image of the cell data sensed by the cell sensing module 16 and displaying the image through the display screen 3, so as to facilitate cell counting.
It should be noted that the cell induction module 16 employs an infrared sensor, the infrared sensor emits infrared rays, and a ray outlet is formed at the bottom end of the light-gathering shade 7, and the infrared rays penetrate through the ray outlet and irradiate the cells in the cell hole to capture the cells.
Further, the power supply device 19 uses a rechargeable low-power battery for supplying power to the device.
Specifically, the data storage module 18 employs a memory card for storing data information of cell count.
Referring to fig. 6 to 10, embodiment 2: a placing groove 11 and a rotating groove 10 are arranged on one side of the shell 1 opposite to the anti-skid sleeve 6, wherein the rotating groove 10 is arranged below the placing groove 11, the rotating groove 10 is arranged at the bottom end of the shell 1, the top of the induction end 8 is rotatably connected with the rotating groove 10 through a damping rotating shaft 13, the shape of the placing groove 11 is matched with the shapes of the induction end 8 and the snoot 7, two arc-shaped grooves 12 are symmetrically arranged on two sides of the upper half section of the placing groove 11 for digging up the induction end 8 and the snoot 7 so as to facilitate the rotation and stacking of the induction end 8 and the snoot 7, opposite corners of the top of the induction end 8 are in arc chamfer transition to form an arc part 14 with smooth surface wall so as to facilitate the rotation of the induction end 8, arc parts matched with the connection part of the induction end 8 and the snoot 7 are symmetrically arranged at the middle positions of two sides of the inner wall of the placing groove 11 so as to facilitate the induction end 8 and the snoot 7 to be placed, after the count, the user can rotate induction end 8 and snoot 7 through damping pivot 13 to rotate induction end 8 and snoot 7 and accomodate to standing groove 11, thereby realize the protection to induction end 8 and snoot 7, simultaneously the effectual occupation space that has reduced equipment, the depositing of the equipment of being convenient for.
In summary, the electrical connections of the electrical components mentioned above are as follows: the display screen 3 is electrically connected with the image processing module 15, the button 4, the image processing module 15, the cell induction module 16 and the data storage module 18 are electrically connected with the main control chip 17, and the main control chip 17 is electrically connected with the power supply device 19.
The utility model discloses a theory of operation and use flow: when the utility model is used, a user holds the shell 1 with one hand and can press the button 4, so that the button 4 touches the main control chip 17 through the connecting rod 9, the main control chip 17 controls the cell induction module 16 to work, the cell induction module 16 emits infrared rays, the infrared rays penetrate through the ray outlet of the light-gathering shade 7 to irradiate the cell in the cell hole on the cell plate, then the main control chip 17 sends the cell data sensed by the cell induction module 16 to the image processing module 15, the image processing module 15 processes the data to generate an image, the image is displayed through the display screen 3, so that the user can master the cell counting condition, meanwhile, the main control chip 17 stores the image data generated by the image processing module 15 into the data storage module 18 to prevent the data from being lost, and in the counting process of the equipment, the power supply device 19 provides a stable power supply for the equipment, therefore, the number of cells in the cell holes on the cell plate can be known in real time, and the further operation of the experiment is facilitated.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (10)

1. The utility model provides an automatic cell counting equipment of portable miniature controllability of non-contact, includes casing (1), the top of casing (1) is fixed with mount pad (5), and the top of casing (1) is located one side of mount pad (5) and installs vice even board (2) that are the arc structure, the inside of mount pad (5) is pegged graft and is had connecting rod (9), and the top of connecting rod (9) is fixed with button (4), simultaneously connecting rod (9) are located the inside one section outside cover of mount pad (5) and are equipped with the spring, the upper surface of vice even board (2) inlays and is equipped with display screen (3), its characterized in that: the inside of casing (1) is provided with built-in cell nucleus induction system (1001), and induction end (8) are installed to the bottom of casing (1), the bottom joint of induction end (8) has snoot (7), built-in cell nucleus induction system (1001) includes image processing module (15), cell induction module (16), main control chip (17), data storage module (18) and power supply unit (19), wherein image processing module (15) are installed in the inside of vice even board (2), cell induction module (16) are installed in the inside of induction end (8), main control chip (17), data storage module (18) and power supply unit (19) are installed respectively in the inside of casing (1).
2. The non-contact portable micro-adjustable automated cell counting device of claim 1, wherein: the shell (1) is of a middle cylindrical structure, and meanwhile, an anti-skid sleeve (6) matched with the shell in shape is bonded on the front surface of the shell (1).
3. The non-contact portable micro-adjustable automated cell counting device of claim 1, wherein: the image processing module (15) adopts an image sensor and is used for generating an image from the cell data sensed by the cell sensing module (16) and displaying the image through the display screen (3) so as to facilitate cell counting.
4. The non-contact portable micro-adjustable automated cell counting device of claim 1, wherein: the cell induction module (16) adopts an infrared sensor, the infrared sensor emits infrared rays, meanwhile, a ray outlet is formed in the bottom end of the light-gathering cover (7), and the infrared rays penetrate through the ray outlet and irradiate cells in a cell hole so as to capture the cells.
5. The non-contact portable micro-adjustable automated cell counting device of claim 1, wherein: the power supply device (19) adopts a rechargeable low-power-consumption battery and is used for supplying power to the equipment.
6. The non-contact portable micro-adjustable automated cell counting device of claim 1, wherein: the data storage module (18) adopts a memory card and is used for storing data information of cell counting.
7. The non-contact portable micro-adjustable automated cell counting device of claim 2, wherein: a placing groove (11) and a rotating groove (10) are formed in one side, opposite to the anti-skidding sleeve (6), of the shell (1), the rotating groove (10) is located below the placing groove (11), the rotating groove (10) is located at the bottom end of the shell (1), and the top of the induction end (8) is rotatably connected with the rotating groove (10) through a damping rotating shaft (13).
8. The non-contact portable micro-adjustable automated cell counting device of claim 7, wherein: the shape of standing groove (11) and the shape phase-match of response end (8) and snoot (7), and two arc walls (12) have been seted up to the bilateral symmetry of the first half section of standing groove (11) to be used for digging response end (8) and snoot (7), be convenient for the rotation of response end (8) and snoot (7) is overlapped.
9. The non-contact portable micro-adjustable automated cell counting device of claim 7, wherein: the opposite corners of the top of the induction end (8) are in arc chamfer transition to form smooth arc parts (14) of the surface wall, so that the induction end (8) can rotate conveniently.
10. The non-contact portable micro-adjustable automated cell counting device of claim 7, wherein: the middle positions of the two sides of the inner wall of the placing groove (11) are symmetrically provided with arc parts matched with the connecting positions of the induction end (8) and the light-gathering cover (7), so that the induction end (8) and the light-gathering cover (7) can be placed in the placing groove (11).
CN201921611244.8U 2019-09-26 2019-09-26 Non-contact portable miniature adjustable automatic cell counting equipment Active CN211713091U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201921611244.8U CN211713091U (en) 2019-09-26 2019-09-26 Non-contact portable miniature adjustable automatic cell counting equipment

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201921611244.8U CN211713091U (en) 2019-09-26 2019-09-26 Non-contact portable miniature adjustable automatic cell counting equipment

Publications (1)

Publication Number Publication Date
CN211713091U true CN211713091U (en) 2020-10-20

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201921611244.8U Active CN211713091U (en) 2019-09-26 2019-09-26 Non-contact portable miniature adjustable automatic cell counting equipment

Country Status (1)

Country Link
CN (1) CN211713091U (en)

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