CN204714811U - A kind of portable detection of nucleic acids equipment - Google Patents
A kind of portable detection of nucleic acids equipment Download PDFInfo
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- CN204714811U CN204714811U CN201520093005.3U CN201520093005U CN204714811U CN 204714811 U CN204714811 U CN 204714811U CN 201520093005 U CN201520093005 U CN 201520093005U CN 204714811 U CN204714811 U CN 204714811U
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- 238000001514 detection method Methods 0.000 title claims abstract description 65
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 38
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 37
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 37
- 238000010438 heat treatment Methods 0.000 claims abstract description 47
- 238000005034 decoration Methods 0.000 claims abstract description 7
- 239000003292 glue Substances 0.000 claims abstract description 6
- 230000000694 effects Effects 0.000 claims description 12
- 239000000428 dust Substances 0.000 claims description 3
- TWNQGVIAIRXVLR-UHFFFAOYSA-N oxo(oxoalumanyloxy)alumane Chemical compound O=[Al]O[Al]=O TWNQGVIAIRXVLR-UHFFFAOYSA-N 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 abstract description 16
- 238000007689 inspection Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 15
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- 229920000742 Cotton Polymers 0.000 description 6
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- 238000002347 injection Methods 0.000 description 5
- 239000012634 fragment Substances 0.000 description 3
- 239000004973 liquid crystal related substance Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- JOYRKODLDBILNP-UHFFFAOYSA-N Ethyl urethane Chemical compound CCOC(N)=O JOYRKODLDBILNP-UHFFFAOYSA-N 0.000 description 2
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The portable detection of nucleic acids equipment of the one that the utility model provides, comprises decoration panel, upper cover plate, upper housing, lower housing, renovates, protects flexible glue, stationary platform, chip pocket, heating detection module, master control board, screen, control circuit board, battery, battery plate, fan, power interface, power switch.Described portable detection of nucleic acids equipment coordinates micro-fluidic chip to use.Described heating detection module has multiple sense channel, and each passage inserts the micro-fluidic chip described in a slice, and multiple passage can carry out reaction detection simultaneously.Described master control board and described screen, described control circuit board form whole Controlling System.The advantage of portable detection of nucleic acids equipment of the present utility model has: small in volume, easy to carry, simple to operate, detected result are directly perceived, is very applicable to inspection and quarantine, Kuai Jiandeng field, battlefield uses.
Description
Technical field
The utility model relates to a kind of detection of nucleic acids equipment, belongs to field of biological detection.
Background technology
Pathogenic bacterium comprise bacterium, virus, spirochete, Rickettsiae, chlamydozoan, mycoplasma, fungi and actinomycetes etc.Detect pathogenic bacterium by nucleic acid and there is advantage that is highly sensitive, that take a blood sample without the need to user.The function that detection of nucleic acids equipment generally has amplification and detects, its principle and technology also have multiple, as PCR fluoroscopic examination, isothermal duplication fluoroscopic examination, isothermal duplication color developing detection etc., as CN101365781A, CN203750554U etc.But ubiquity is with high costs, the large heaviness of volume, complicated operation, cannot automatic interpretation, the shortcoming high to user's competency profiling.
Currently marketed detection of nucleic acids unit type is a lot, the temperature control mode of its nucleic acid amplification has the modes such as air heating cooling, semiconductor chilling plate temperature control, heating film temperature control, infrared temp. control, but reagent and sample are generally react inside PCR pipe, have that power supply energy loss is large, reagent and the large shortcoming of sample consumption, and Testing index is single or it is single to detect sample, is difficult to accomplish detection that is portable and multi objective multiple sample.
Realize the detection accomplishing multi objective multiple sample on portable basis, need that temperature control area is little, the thermal capacitance of temperature control target is little to reduce the loss of energy, need to use novel reagent sample reaction carriers to realize the detection of multi objective multiple sample, such as micro-fluidic chip.This it is also proposed new requirement to detection of nucleic acids equipment.
Summary of the invention
The utility model provides a kind of portable detection of nucleic acids equipment and using method thereof, and supporting micro-fluidic chip uses, and assists without the need to other laboratory equipments, user operation is simple, automatically provide sentence read result, small in volume, be very applicable to inspection and quarantine, Kuai Jiandeng field, battlefield uses.
An object of the present utility model is to provide a kind of portable detection of nucleic acids equipment.
The portable detection of nucleic acids equipment of the one that the utility model provides, is characterized in that, comprises renovating, heating detection module, master control board, screen, control circuit board.
Wherein, described portable detection of nucleic acids equipment is provided with chip pocket.Described chip pocket is connected with heating detection module.
More specifically; a kind of portable detection of nucleic acids equipment, comprises decoration panel, upper cover plate, upper housing, lower housing, renovates, protects flexible glue, stationary platform, chip pocket, heating detection module, master control board, screen, control circuit board, battery, battery plate, fan, power interface, power switch.
Above-mentioned portable detection of nucleic acids equipment adopts the principle of isothermal duplication color developing detection, obtains the coloured image of conversion zone and carry out automatic analysis, provide sentence read result during detection.
Above-mentioned portable detection of nucleic acids equipment, supporting micro-fluidic chip uses.
Described micro-fluidic chip can be multiple pattern, but need ensure that reaction tank region is plane, to facilitate Image Acquisition.Such as can use the micro-fluidic chip in patent " a kind of new microfluidic chip and using method thereof " (application number: CN201310230172.3).As required, the micro-fluidic chip of other patterns can also be used.
Reagent is stored in reaction tank on described micro-fluidic chip, after sample is injected described micro-fluidic chip, again described micro-fluidic chip is inserted in described chip pocket, described heating detection module can heat the reaction tank on described micro-fluidic chip, and obtain the coloured image of conversion zone, software afterwards on described master control board is analyzed image, finally judges detected result, and display on the screen.
Described decoration panel plays the effect of decoration, its internal surface character silk printing and mark, does not reach silk-screen layer during actual use, and therefore word and mark can not be worn and torn.
Described upper cover plate plays the effect of closed whole device housings.
Described upper housing and described lower housing are used for the effect of load bearing equipment inner components, and both to form a complete handle in side after assembling, convenient during to make whole equipment portable.
Described renovates for shutting out the light, need to renovate during use to open, then could insert described micro-fluidic chip, cover described renovating when reaction detection, described renovating also has an effect to be block extraneous dust and foreign matter, in order to avoid pollute instrument internal.
Described protection flexible glue is the guard circle of a softwood matter, and material can be various rubber, urethane and so on, for the protection of the shell of equipment, avoids being damaged, also can play the effect of buffering collision of closed and vibration.
Described stationary platform is installation, the support platform of device interior, and most of component and shell are all mounted thereto.
Described micro-fluidic chip is a transparent sheet glass or plastic sheet, adopt multilayered structure, inside responds pond, trickle runner, well, venting hole, reaction tank inside prestores reagent, in use, after the sample added from well and reagent react, the mixed solution in reaction tank presents distinct colors.
Described chip pocket is for hiding described heating detection module, and as the liner that described micro-fluidic chip inserts, described chip pocket is equipped with LED light, the quantity of LED light is identical with sense channel quantity, and LED position and sense channel one_to_one corresponding, for pointing out the running condition of each passage.
Described heating detection module is used for the heating of described micro-fluidic chip and thermostatic control, and can obtain the coloured image of conversion zone, and image is sent to described master control board.Described heating detection module comprises at least 1 sense channel, each sense channel detects the micro-fluidic chip described in 1 at every turn, each passage comprises 1 colour imagery shot, 1 heating film, 1 temperature sensor, 1 heat-conducting plate, and whole module also has at least 1 LED lamp bar to be used for illumination, at least 1 heating control circuit plate in addition.LED lamp bar is preferably two.Hyperchannel can detect by simultaneous reactions, detects demand while meeting many person-portions sample.
Each passage uses independent colour imagery shot, heating film, temperature sensor, heat-conducting plate.Described LED lamp bar, described colour imagery shot are connected with described control circuit board, and described heating film, described temperature sensor are connected with described thermal control plate, and described thermal control plate is connected with described control circuit board.
All sense channels can be covered after on the described lid renovated, there is the effect of blocks dust and shading.
Described screen, described control circuit board are connected with described master control board.
Described heat-conducting plate, the one side towards described colour imagery shot adopts the true qualities aluminum oxide of frosted finish effect.
Described master control board is mounted with operating system, as upper computer in whole equipment, for the treatment of the data detected, and the USB interface, the network interface that provide equipment to be connected with the external world, also be connected with described screen and described control circuit board in addition, receive the signal that the touch-screen on described screen sends, the display controlling liquid crystal display.
Described screen comprises touch-screen and liquid crystal display, and touch-screen is used for the touch control operation of operation response personnel and outputs signal, and liquid crystal display is used for display, and commercially, both has encapsulation all-in-one-piece, also has split to sell.
Described control circuit board in whole equipment as lower computer, be responsible for the power management of equipment, with described master control board communication, control described in heating detection module in heating control circuit plate, control described in heating detection module in camera, the running that controls described fan run for the extraction module described in dispelling the heat to device, controlling.
Described battery is used for power supply to the device, use of can repeatedly charging.
Described battery plate is used for stationary battery.
Described fan is used for ventilating, and dispels the heat to the device of device interior.
The effect of described power interface is that external power adapter is by this interface power supply to the device or to the charging of described battery.
Described power switch controls whole equipment and opens and close.
Second object of the present utility model is to provide the using method of the portable detection of nucleic acids equipment that above-mentioned first object provides.
The method that the utility model provides, comprises the steps:
1) sample (as Nasopharyngeal swabs) is added as described in micro-fluidic chip;
2) open renovating on portable detection of nucleic acids equipment, micro-fluidic chip is inserted from slot, then covers and renovate.
3) parameter of reaction is set by touch-screen on screen, then clicks corresponding icon and start reaction.
4), after having reacted until screen prompt, detecting and terminate, the detected result of each passage can have been checked.
According to above technical scheme and using method, above-mentioned portable detection of nucleic acids equipment meets the demand of portable detection completely:
1, little, lightweight, the jug of volume, easy to carry.
2, reagent and sample consumption little.
3, automatic interpretation, to operator's operant level without particular requirement.
4, efficiency is high, and many person-portions multi objective detects simultaneously.
5, cost is low, adopts ordinary student production art namely to can be mass.
Accompanying drawing explanation
Fig. 1 is the portable detection of nucleic acids equipment appearance schematic diagram in embodiment 1.
Fig. 2 is the portable detection of nucleic acids equipment component composition schematic diagram in embodiment 1.
Fig. 3 is the portable detection of nucleic acids equipment inner structure schematic diagram in embodiment 1.
Fig. 4 is the portable detection of nucleic acids equipment inner structure partial schematic diagram in embodiment 1.
Fig. 5 is the micro-fluidic chip schematic diagram in embodiment 1.
Fig. 6 is the heating detection module schematic diagram in embodiment 1.
Fig. 7 is the heating detection module part composition schematic diagram in embodiment 1.
Fig. 8 is the partial schematic diagram that the chip in embodiment 1 is inserted into heating detection module.
Wherein, description of reference numerals is as follows:
101 decoration panels, 102 upper cover plates, 103 upper housings, 104 lower housings, 105 renovate, 106 protection flexible glues, 107 stationary platforms, 201 micro-fluidic chips, 108 chip pocket, 502 master control boards, 501 screens, 503 control circuit boards, 504 batteries, 505 battery plates, 506 fans, 507 power interfaces, 508 power switches, 300 heating detection modules, 301 supports, 302 lens supporting structures, 303 heat-conducting plate anchors, 304 colour imagery shots, 305 heat-conducting plates, 306 temperature sensors, 307 heating films, 308 insulating cotton, 309 heating film cover plates, 310LED lamp bar, 311 shell fragments, 312 heating control circuit plates, 400 extraction modules, 401 motor racks, 402 motors, 403 heating tubes, 404 heating films, 405 insulating cotton, 406 magnetic receivers, 407 magnet.
Embodiment
The working method used in following embodiment if no special instructions, is ordinary method.
Material used in following embodiment, device etc., if no special instructions, all can obtain from commercial channels.
In following embodiment, the production method of part is routine techniques and the method for mechanical processing industry and plastics Injection Industry.
Below in conjunction with specific embodiments and the drawings, the utility model is described in further detail, but content of the present utility model is not limited to embodiment.
Decoration panel 101 in the portable detection of nucleic acids equipment of the present embodiment adopts PMMA or PC material, upper cover plate 102, upper housing 103, lower housing 104, renovate 105 all adopt ABS material (as shown in Figure 1), to polish after the processing of these parts can adopt digital control processing the mode of spraying paint, also can adopt the mode of mold injection, be all the general production method of plastic working industry.
Protection flexible glue 106 adopts the mode of mold injection, and material is silica gel or rubber, urethane (as shown in Figure 2).Micro-fluidic chip 201 adopts PMMA material (as shown in Figure 5), produces by the mode of mold injection, and concrete using method is see patent " a kind of new microfluidic chip and using method thereof " (application number: CN201310230172.3).
Stationary platform 107, support 301, lens supporting structure 302, heat-conducting plate 305, motor rack 401, these parts of heating tube 403 adopt aluminum alloy material (as shown in Figure 3).Wherein the surface treatment of heat-conducting plate 305 adopts true qualities oxidation, and surface needs frosted finish effect, and clean uniform thin frosted finish effect can promote the quality of detected image.The complete processing of these parts is all the ordinary process of mechanical processing industry.
Chip pocket 108, heat-conducting plate anchor 303, heating film cover plate 309, battery plate 505 adopt POM material (as shown in Figure 4), wherein chip pocket 108, heat-conducting plate anchor 303 need the POM material adopting black, chip pocket 108 due to outward appearance visible, so its surface needs flat smooth.These parts can adopt the mode of mechanical workout also can adopt the mode of mold injection, and complete processing is all the common technique of mechanical processing industry and plastic working industry.
Shell fragment 311 uses spring steel material (as shown in Figure 8), and surface treatment becomes black to contribute to promoting the quality of detected image, and magnetic receiver 406 adopts the material of Q235 steel or other energy magnetic, and antirust treatment is made on surface.The complete processing of these parts is the common technique of mechanical processing industry.
The raw material that heating control circuit plate 312, control circuit board 503 adopt market common and device, welding is without particular requirement.
Colour imagery shot 304, temperature sensor 306, heating film 307, insulating cotton 308, LED lamp bar 310, magnet 407, motor 402, heating film 404, insulating cotton 405, screen 501, master control board 502, battery 504, fan 506, power interface 507, power switch 508, these devices are all from purchasing on the market, wherein camera 304 adjustable focal length, screen 501 comprises touch-screen and display screen, existing split also has the product be packaged together on the market, master control board 502 adopts the industrial control mainboard of small volume or embedded industrial control board, adopt Windows system or WinCE system, android system, linux system can.
300 heating detection modules are by support 301, lens supporting structure 302, heat-conducting plate anchor 303, colour imagery shot 304, heat-conducting plate 305, temperature sensor 306, heating film 307, insulating cotton 308, heating film cover plate 309, LED lamp bar 310, shell fragment 311, composition (as shown in Figure 6) installed by heating control circuit plate 312, comprise multiple sense channel, each sense channel is by 1 colour imagery shot 304, 1 heat-conducting plate 305, 1 temperature sensor 306, 1 heating film 307, 1 spring piece 311 forms, the heat-conducting plate 305 of the most accurate respective channel when colour imagery shot 304 is installed, and clear picture during adjusting focal length guarantee insertion micro-fluidic chip 201.Temperature sensor 306 is contained in the groove of heat-conducting plate 305 one side after smearing heat-conducting silicone grease, then heating film 307 is sticked in this one side, stick insulating cotton 308 after each passage installation, then cover heating film cover plate 309 and fix tight, finally heating control circuit plate 312 is installed and wiring.(as shown in Figure 7)
The method that the utility model provides, comprises the steps:
1) sample (as Nasopharyngeal swabs extract) is added as described in micro-fluidic chip 201;
2) open and portable detection of nucleic acids equipment renovates 105, micro-fluidic chip 201 is inserted from slot 108, then covers and renovate 105;
3) parameter of reaction is set by touch-screen 501 on screen, then clicks corresponding icon and start reaction;
4), after having reacted until screen prompt, detecting and terminate, the detected result of each passage can have been checked.
Above content is in conjunction with concrete preferred implementation further detailed description of the utility model, can not assert that concrete enforcement of the present utility model is confined to these explanations.For the utility model person of an ordinary skill in the technical field, without departing from the concept of the premise utility, some simple deduction or replace can also be made, all should be considered as belonging to protection domain of the present utility model.
Claims (10)
1. a portable detection of nucleic acids equipment, is characterized in that, comprises renovating, heating detection module, master control board, screen, control circuit board.
2. portable detection of nucleic acids equipment according to claim 1, is characterized in that, be provided with chip pocket.
3. portable detection of nucleic acids equipment according to claim 2, is characterized in that, described chip pocket is connected with heating detection module.
4. portable detection of nucleic acids equipment according to claim 3, it is characterized in that, heating detection module comprises at least 1 sense channel and at least 1 LED lamp bar, at least 1 heating control circuit plate, and each passage uses 1 chip, described chip-and be micro-fluidic chip.
5. portable detection of nucleic acids equipment according to claim 4, it is characterized in that, each passage uses independent colour imagery shot, heating film, temperature sensor, heat-conducting plate.
6. portable detection of nucleic acids equipment according to claim 5, it is characterized in that, described LED lamp bar, described colour imagery shot are connected with described control circuit board, described heating film, described temperature sensor are connected with described thermal control plate, and described thermal control plate is connected with described control circuit board.
7. portable detection of nucleic acids equipment according to claim 1, is characterized in that, described on the lid renovated after can cover all sense channels, there is the effect of blocks dust and shading.
8. portable detection of nucleic acids equipment according to claim 1, is characterized in that, described screen, described control circuit board are connected with described master control board.
9. portable detection of nucleic acids equipment according to claim 5, is characterized in that, described heat-conducting plate, and the one side towards described colour imagery shot adopts the true qualities aluminum oxide of frosted finish effect.
10. the portable detection of nucleic acids equipment according to the arbitrary claim of claim 1-9; it is characterized in that, described portable detection of nucleic acids equipment also comprises decoration panel, upper cover plate, upper housing, lower housing, protection flexible glue, stationary platform, battery, battery plate, fan, power interface, power switch etc.
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Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106834110A (en) * | 2015-12-04 | 2017-06-13 | 北京倍肯华业科技发展有限公司 | A kind of isothermal PCR detector and its detection method |
CN107541458A (en) * | 2016-06-24 | 2018-01-05 | 广州康昕瑞基因健康科技有限公司 | Mask |
CN107955778A (en) * | 2017-11-19 | 2018-04-24 | 杭州安弼晟生物科技有限公司 | Novel molecular diagnostic device |
CN108531390A (en) * | 2018-04-11 | 2018-09-14 | 张薇 | A kind of portable equipment that can be used for Site Detection nucleic acid |
CN110358681A (en) * | 2019-07-09 | 2019-10-22 | 成都万众壹芯生物科技有限公司 | A kind of portable PCR device |
CN110408529A (en) * | 2019-07-12 | 2019-11-05 | 北京资和源医疗科技有限公司 | Linear scanning formula PCR instrument and the method for carrying out PCR detection using it |
CN111560310A (en) * | 2020-05-29 | 2020-08-21 | 上海交通大学 | Random access type digital nucleic acid detection device and use method |
CN111647498A (en) * | 2020-05-25 | 2020-09-11 | 清华大学 | Integrated self-service nucleic acid detection device and use method thereof |
CN112673090A (en) * | 2018-10-29 | 2021-04-16 | Nok株式会社 | Micro-fluidic chip and micro-fluidic device |
CN113150979A (en) * | 2020-12-30 | 2021-07-23 | 北京航空航天大学 | Portable nucleic acid detector based on micropore array chip |
CN113930322A (en) * | 2021-10-12 | 2022-01-14 | 江苏汇先医药技术有限公司 | LAMP detector and control method |
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2015
- 2015-02-09 CN CN201520093005.3U patent/CN204714811U/en active Active
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106834110A (en) * | 2015-12-04 | 2017-06-13 | 北京倍肯华业科技发展有限公司 | A kind of isothermal PCR detector and its detection method |
CN107541458A (en) * | 2016-06-24 | 2018-01-05 | 广州康昕瑞基因健康科技有限公司 | Mask |
CN107955778A (en) * | 2017-11-19 | 2018-04-24 | 杭州安弼晟生物科技有限公司 | Novel molecular diagnostic device |
CN108531390A (en) * | 2018-04-11 | 2018-09-14 | 张薇 | A kind of portable equipment that can be used for Site Detection nucleic acid |
CN112673090A (en) * | 2018-10-29 | 2021-04-16 | Nok株式会社 | Micro-fluidic chip and micro-fluidic device |
CN110358681A (en) * | 2019-07-09 | 2019-10-22 | 成都万众壹芯生物科技有限公司 | A kind of portable PCR device |
CN110408529A (en) * | 2019-07-12 | 2019-11-05 | 北京资和源医疗科技有限公司 | Linear scanning formula PCR instrument and the method for carrying out PCR detection using it |
CN111647498A (en) * | 2020-05-25 | 2020-09-11 | 清华大学 | Integrated self-service nucleic acid detection device and use method thereof |
CN111560310A (en) * | 2020-05-29 | 2020-08-21 | 上海交通大学 | Random access type digital nucleic acid detection device and use method |
CN111560310B (en) * | 2020-05-29 | 2023-01-03 | 上海交通大学 | Random access type digital nucleic acid detection device and use method |
CN113150979A (en) * | 2020-12-30 | 2021-07-23 | 北京航空航天大学 | Portable nucleic acid detector based on micropore array chip |
CN113930322A (en) * | 2021-10-12 | 2022-01-14 | 江苏汇先医药技术有限公司 | LAMP detector and control method |
CN113930322B (en) * | 2021-10-12 | 2024-03-08 | 江苏汇先医药技术有限公司 | LAMP detector and control method |
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