CN113930322B - LAMP detector and control method - Google Patents
LAMP detector and control method Download PDFInfo
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- CN113930322B CN113930322B CN202111209767.1A CN202111209767A CN113930322B CN 113930322 B CN113930322 B CN 113930322B CN 202111209767 A CN202111209767 A CN 202111209767A CN 113930322 B CN113930322 B CN 113930322B
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- 238000000034 method Methods 0.000 title claims abstract description 18
- 238000001514 detection method Methods 0.000 claims abstract description 157
- 238000010438 heat treatment Methods 0.000 claims abstract description 67
- 230000003287 optical effect Effects 0.000 claims abstract description 56
- 230000007246 mechanism Effects 0.000 claims abstract description 27
- 238000012921 fluorescence analysis Methods 0.000 claims abstract description 15
- 229920000742 Cotton Polymers 0.000 claims description 11
- 238000007639 printing Methods 0.000 claims description 8
- 238000004458 analytical method Methods 0.000 claims description 4
- 230000003993 interaction Effects 0.000 claims description 2
- 238000009413 insulation Methods 0.000 claims 3
- 230000003321 amplification Effects 0.000 abstract description 15
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 15
- 150000007523 nucleic acids Chemical class 0.000 abstract description 14
- 102000039446 nucleic acids Human genes 0.000 abstract description 12
- 108020004707 nucleic acids Proteins 0.000 abstract description 12
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- 230000001717 pathogenic effect Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000001228 spectrum Methods 0.000 description 4
- 238000005056 compaction Methods 0.000 description 3
- 239000013039 cover film Substances 0.000 description 3
- 238000001917 fluorescence detection Methods 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 238000011901 isothermal amplification Methods 0.000 description 3
- 230000001154 acute effect Effects 0.000 description 2
- 238000004891 communication Methods 0.000 description 2
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- 238000010183 spectrum analysis Methods 0.000 description 2
- 238000007397 LAMP assay Methods 0.000 description 1
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- 238000000338 in vitro Methods 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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Abstract
The invention discloses an LAMP detector and a control method. The control method comprises the following steps: A. acquiring the electric quantity of the battery, and executing the following steps if the electric quantity is larger than or equal to a set threshold value; B. the bar code scanning method comprises the steps of scanning a bar code to read bar code information, wherein the bar code information comprises sample information and/or user information; C. receiving a detection instruction of a user through a touch screen; D. the driving mechanism resets the optical detection device and heats the heating component at constant temperature; E. the driving mechanism enables the optical detection device to move to the first reaction bin and reads the detection result; F. the driving mechanism enables the optical detection device to move to the next reaction bin and reads the detection result; G. judging the fluorescence analysis value sent by the optical detection device to obtain the detection result of the detection item corresponding to each reaction bin; H. and sending the detection result to the touch screen for display. The LAMP detector can integrate automatic constant-temperature heating, nucleic acid amplification detection and detection result display, and can also be used for handheld detection.
Description
The application is a divisional application with the application number 202111187960X, named hand-held LAMP detector and System, filed on 10 months 12 of 2021.
Technical Field
The invention belongs to the technical field of LAMP detection, and relates to an LAMP detector and a control method.
Background
The LAMP (Loop-mediated isothermal amplification) technology is widely applied to the field of biological diagnosis due to the advantages of mild reaction conditions (lower reaction temperature), short reaction time and the like, such as nucleic acid amplification detection to diagnose whether pathogens exist in a sample. The LAMP technology is a process of obtaining a nucleic acid amplification result by providing in-vitro amplification conditions for a nucleic acid fragment, exponentially amplifying the nucleic acid fragment in large quantity, adding a fluorescent dye or a fluorescent marker in the nucleic acid amplification process, detecting the intensity of a fluorescent signal by adopting an optical device, and analyzing the fluorescent signal. In the nucleic acid amplification reaction, the reaction system needs to be heated. The steps in the detection process are integrated into the LAMP detector to the greatest extent and automatically completed by the LAMP detector, so that the operation convenience and the detection efficiency are improved, and the LAMP detector is one of targets of LAMP detector designers.
Disclosure of Invention
The invention provides an LAMP detector which can integrate automatic constant-temperature heating, nucleic acid amplification detection and detection result display, and can be used for handheld detection.
The invention also provides a control method of the LAMP detector, which can enable the hand-held LAMP detector to automatically heat at constant temperature, detect nucleic acid amplification and display detection results.
According to a first aspect of the present invention, a LAMP-based detector includes a chip holder for accommodating a LAMP chip, a heating assembly for heating the LAMP chip at constant temperature, an optical detection device movable in a left-right direction, a driving mechanism for driving the optical detection device to move in the left-right direction, a touch screen serving as a man-machine interface, a battery for supplying power, and a controller,
the controller is used for detecting the electric quantity of the battery, comparing the electric quantity with a set threshold value and normally operating when the electric quantity is greater than or equal to the set threshold value;
the touch screen is provided with a plurality of display states, and in one display state, the touch screen is provided with an area for a user to input instructions, and the touch screen is used for receiving a start detection instruction of the user in the display state and sending the start detection instruction to the controller; the controller is also used for sending a control signal for resetting the optical detection device to a starting position and a control signal for heating the heating component at constant temperature to the driving mechanism after receiving a start detection instruction sent by the touch screen;
the controller is further used for sending a control signal for enabling the optical detection device to move to a certain reaction bin corresponding to the LAMP chip to the driving mechanism; the optical detection device is used for carrying out laser irradiation and fluorescence collection and analysis on the opposite reaction bin and sending a fluorescence analysis value to the controller; the controller is also used for judging the negative and positive of the detection item corresponding to the reaction bin according to the fluorescence analysis value sent by the optical detection device and the prestored interpretation standard;
and the controller is also used for sending the interpreted detection result to the touch screen for display.
In an embodiment, the LAMP detector further comprises a printer, and the controller is further configured to send the interpreted detection result to the printer for printing.
In an embodiment, the LAMP detector further comprises a code scanner, and the controller is further configured to receive barcode information read by the code scanner and correlate the barcode information with the detection result.
In an embodiment, the heating assembly includes a heat-conducting plate, a heating film, heat-insulating cotton, a temperature sensor and a fuse, wherein the heat-insulating cotton is arranged on the chip holder, the heating film is arranged on the heat-insulating cotton, the heat-conducting plate is arranged on the heating film, the temperature sensor is electrically connected with the controller, the heating film is electrically connected with the controller through the fuse, and the controller is further used for receiving a heating temperature value returned by the temperature sensor, comparing the heating temperature value with a set temperature value and controlling the heating temperature of the heating film according to a comparison result.
In an embodiment, the driving mechanism includes a guide rail extending in a left-right direction, a mounting block movably disposed on the guide rail in the left-right direction, and a motor for driving the mounting block to move in the left-right direction, the optical detection device is disposed on the mounting block, the motor is electrically connected with the controller, and the motor is used for responding to a control signal sent by the controller to the driving mechanism to rotate by a corresponding angle.
In an embodiment, the battery is a rechargeable battery, and the LAMP detector includes a power interface for accessing an external power source, and the power interface is electrically connected to the controller.
In an embodiment, the controller is configured to send an alarm signal when the power is less than the set threshold.
According to a second aspect of the present invention, a control method of the LAMP detector as described above, comprises the steps of:
A. acquiring the electric quantity of the battery, judging whether the electric quantity is larger than or equal to a set threshold value, and if so, executing the following steps;
B. the method comprises the steps of scanning a code to read bar code information, wherein the bar code information comprises sample information and/or user information;
C. receiving a detection instruction of a user through a touch screen;
D. the driving mechanism resets the optical detection device and heats the heating component at constant temperature;
E. the driving mechanism enables the optical detection device to move to the first reaction bin and reads the detection result of the first reaction bin;
F. the driving mechanism enables the optical detection device to move to the next reaction bin and reads the detection result of the next reaction bin;
repeating the step F until all the reaction bins are read;
G. judging the fluorescence analysis value sent by the optical detection device to obtain the detection result of the detection item corresponding to each reaction bin;
H. and sending the detection result to the touch screen for display.
In one embodiment, in step a, if the result is no, a low power alarm signal is generated through the touch screen; and/or, the LAMP detector is powered by an external power supply and charges a battery.
In one embodiment, in step H, the detection result is also sent to the printer for printing.
Compared with the prior art, the invention has the following advantages:
the LAMP detector is powered by a battery, can integrate automatic constant-temperature heating, nucleic acid amplification and detection, automatic code scanning and detection result display, can be used for handheld detection, and is convenient to carry and use; the user can automatically complete isothermal heating amplification and fluorescence detection analysis and detection structure display by loading the LAMP chip, so that the detection efficiency is improved.
Drawings
In order to more clearly illustrate the technical solutions of the present invention, the drawings that are needed in the description of the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
Fig. 1 is a schematic perspective view of an LAMP detection system according to an embodiment of the present invention.
Fig. 2 is a schematic perspective view of the LAMP detection system according to the embodiment of the present invention at another view angle.
Fig. 3 is a schematic diagram showing an internal structure of the LAMP detector according to the embodiment of the present invention at a viewing angle, in which LAMP chips are inserted.
Fig. 4 is a schematic diagram showing the internal structure of the LAMP detector according to the embodiment of the present invention at another view angle, in which the LAMP chip has been inserted.
Fig. 5 is an exploded schematic view of a LAMP chip, a chip holder, and a heating assembly.
Fig. 6 is a schematic diagram of a coating film of the first surface of the LAMP chip according to an embodiment of the present invention.
Fig. 7 is a schematic diagram of a coating of the second surface of the LAMP chip according to an embodiment of the present invention.
Fig. 8 is a schematic perspective view of a chip body.
Fig. 9 is a schematic view of the first surface of the chip body.
Fig. 10 is a schematic diagram of the second surface of the chip body.
Fig. 11 is a side view of a chip body.
Fig. 12 is a block diagram of a control system of the LAMP detector according to the embodiment of the present invention.
Fig. 13 is a flowchart of a control flow of the LAMP detector according to the embodiment of the present invention.
Wherein,
1. a housing; 10. a chip port; 11. a power interface; 12. a recessed portion;
2. a chip holder; 21. a fixing part; 21a, a slot; 22. a support part; 22a, a support surface;
3. a heating assembly; 31. a heat conductive plate; 32. heating the film; 33. a temperature sensor; 34. a fuse; 35. compressing the spring plate;
4. an optical detection device; 41. a spectral sensor;
5. a driving mechanism; 51. a guide rail; 52. a mounting block; 53. a motor; 54. a screw rod; 55. an optical coupler;
61. a battery; 610. a battery case; 62. a touch screen; 63. a printer; 64. a code scanner;
7. a LAMP chip; 70. a chip body; 70a, a first surface; 70b, a second surface; 701. a sample adding port; 702. an overflow trough; 703. an exhaust port; 704. a reaction bin; 705. a first microchannel; 705a, a first arc-shaped bending part; 705b, a second arc-shaped bending part; 706. a second microchannel; 707. a groove; 708. unfilled corners; 71. coating a film; 72. a first sealing film; 73. a second sealing film;
81. a first control chip; 82. and a second control chip.
Detailed Description
Preferred embodiments of the present invention will be described in detail below with reference to the attached drawings so that the advantages and features of the present invention can be more easily understood by those skilled in the art. The description of these embodiments is provided to assist understanding of the present invention, but is not intended to limit the present invention.
In the description of the present application, it should be understood that the terms "upper," "lower," "left," "right," "front," "rear," "vertical," "horizontal," "inner," and "outer," etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, merely to facilitate description of the present application and simplify the description, and do not indicate or imply that the device or element being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present application. The directional terms mentioned herein are used with reference to the arrows shown in fig. 3.
The embodiment provides a handheld LAMP detector and a handheld LAMP detection system, which are used for detecting nucleic acid. Referring to fig. 1 and 2, the hand-held LAMP detection system includes the LAMP detector and LAMP chip 7 described above. The LAMP detector includes a housing 1, and an inner cavity for mounting an internal member is formed in the housing 1. The front side wall of the housing 1 is provided with a chip port 10 into which the LAMP chip 7 is inserted, and the LAMP chip 7 is inserted into the LAMP detector from the chip port 10. The internal structure of the LAMP detector is shown in fig. 3 to 5, in which the housing 1 is not shown. Referring to fig. 3 to 5, the LAMP detector further includes a chip holder 2, a heating unit 3, an optical detection device 4, a driving mechanism 5, a battery 61, a touch screen 62, a printer 63, a code scanner 64, a control system, and the like. The chip opening 10 is arranged on the front side wall of the shell 1, the chip seat 2 is positioned at the front part of the inner cavity, the battery 61 is positioned at the rear side of the inner cavity, the heating component 3 is arranged on the chip seat 2, at least part of the optical detection device 4 is positioned above the chip seat 2, and the driving mechanism 5 is arranged between the chip seat 2 and the battery 61. Fig. 6 to 11 show a LAMP chip 7 of the hand-held LAMP detection system, the LAMP chip 7 having a plurality of reaction cartridges 704 juxtaposed in the left-right direction.
As shown in fig. 3 to 5, the chip holder 2 is disposed in the inner cavity, the chip holder 2 has a receiving space for receiving the LAMP chip 7, the receiving space is opposite to the chip port 10, and the LAMP chip 7 is horizontally inserted into the receiving space from the chip port 10. Specifically, the chip carrier 2 includes a fixing portion 21 and a supporting portion 22 disposed at a rear side of the fixing portion 21, and the chip carrier 2 is integrally formed, such as integrally injection-molded plastic. The accommodating space includes a slot 21a provided on the fixing portion 21 for the LAMP chip 7 to penetrate, the slot 21a penetrating through the fixing portion 21 in the front-rear direction; the support 22 has a support surface 22a for facing the rear of the LAMP chip 7, and the heating element 3 is provided on the support surface 22 a. Further, the slot 21a is substantially horizontal and flush with the chip opening 10, and is directly opposite to the slot; the supporting surface 22a is specifically located on the upper surface of the supporting portion 22, the heating element 3 is disposed on the supporting surface 22a, and after the LAMP chip 7 is inserted, the rear portion of the LAMP chip 7 (i.e. the portion having the reaction chamber 704) is located on the heating element 3, and the two are attached to each other or close to each other, and ideally attached to each other, so as to heat the system in the reaction chamber 704 of the LAMP chip 7, and perform isothermal amplification. The chip carrier 2 is fixed to the lower side wall of the front part of the housing 1.
As shown in fig. 3 to 5, the heating component 3 is configured to perform constant temperature heating on the LAMP chip 7, and the heating component 3 is disposed in the inner cavity of the housing 1, and further disposed on the supporting surface 22a of the chip holder 2. Specifically, as shown in fig. 5, the heating assembly 3 includes a heat-conducting plate 31, a heating film 32, heat-insulating cotton, a temperature sensor 33 and a fuse 34, the heat-insulating cotton is disposed on the supporting surface 22a of the chip carrier 2, the heating film 32 is disposed on the heat-insulating cotton, the heat-conducting plate 31 is disposed on the heating film 32, and the temperature sensor 33 and the fuse 34 are embedded in the heat-insulating cotton. Further, the heat conductive plate 31, the heating film 32, and the insulating cotton are laminated on the supporting surface 22a of the chip carrier 2 from top to bottom. The fuse 34 and the heating film 32 are connected in series for overcurrent protection. The heating assembly 3 further comprises a pressing spring piece 35 for pressing the LAMP chip 7 on the heat conducting plate 31, and the pressing spring piece 35 is fixedly arranged on the mounting seat. The number of the pressing spring pieces 35 is plural and is set at intervals in the left-right direction, and the pressing spring pieces 35 can be elastically deformed. The whole U type that is the opening upwards that is of compaction shell fragment 35, after LAMP chip 7 inserts, compaction shell fragment 35 received LAMP chip 7's extrusion and upwards bends parallelly, under the elastic force effect, the lower tip of compaction shell fragment 35 supports and compresses tightly it on heat conduction board 31 at LAMP chip 7 to make the two laminating, have better heating effect.
As shown in fig. 3 and 4, the optical detection device 4 is provided in the cavity so as to be movable in the left-right direction. The optical detection device 4 has a plurality of detection positions for respectively corresponding to the plurality of reaction bins 704 on the LAMP chip 7. During detection, the optical detection device 4 moves to a detection position to detect a corresponding reaction bin 704; after the detection, the optical detection device 4 continues to move to the next detection position to detect the corresponding other reaction chamber 704. The optical detection device 4 employs a known optical device capable of emitting a laser beam for irradiating the system in the reaction chamber 704 to excite fluorescence, and collecting and performing spectral analysis of the excited fluorescence. The optical detection device 4 of the present embodiment preferably employs a monochromatic laser light source; the optical detection device 4 further comprises a spectral sensor for performing a spectral analysis of the excited fluorescence.
As shown in fig. 3 and 4, the driving mechanism 5 is used for driving the optical detection device 4 to move in the left-right direction, and the driving mechanism 5 is disposed in the inner cavity. Specifically, the driving mechanism 5 includes a guide rail 51 provided on an inner wall (specifically, a lower side wall) of the housing 1 and extending in the left-right direction, a mounting block 52 provided on the guide rail 51 so as to be movable in the left-right direction, and a motor 53 for driving the mounting block 52 to move in the left-right direction, the motor 53 being provided on the inner wall of the housing 1, and the optical detection device 4 being provided on the mounting block 52. Further, the motor 53 drives a screw rod 54 extending in the left-right direction to rotate, the screw rod 54 is connected to the mounting block 52 through a nut, and the mounting block 52 moves in the left-right direction along with the rotation of the screw rod 54, so as to drive the optical detection device 4 to move left and right.
As shown in fig. 3 and 4, a battery 61 is provided in the inner cavity of the housing 1 for supplying power to the heating assembly 3, the optical detection device 4, the driving mechanism 5, the touch screen 62, the printer 63, the code scanner 64, the control system, and the like. Specifically, the battery 61 is provided in a battery 61 case, and the battery 61 case is provided on the inner wall of the housing 1. The battery 61 is a rechargeable battery 61, and a power interface 11 (as shown in fig. 2) electrically connected to the battery 61 is disposed on the rear side of the housing 1, the power interface 11 can be connected to an external power source, and the LAMP detector can be directly powered by the external power source.
As shown in fig. 1 to 4, the printer 63 and the touch screen 62 are embedded in the upper side wall of the casing 1 and above the battery 61, the touch screen 62 is located at the front side of the printer 63, the printer 63 can print out information such as a detection result, and the touch screen 62 can display information such as a detection result. One of the functions of the touch screen 62 is to display information, and the other function is to serve as a human-computer interaction interface for a user to input instructions. Accordingly, the touch screen 62 has a plurality of display states, and in one display state, the touch screen 62 has an instruction input area for a user to input an instruction, for example, has a "start detection" button; in the other display state, the touch panel 62 has a detection result display area for displaying a detection result, such as "negative" or "positive".
As shown in fig. 1, 3 and 4, the code scanner 64 is embedded on the front side wall of the housing 1 and is located on the right side or the left side of the chip holder 2, and can read the bar code on the LAMP chip 7 to obtain code scanning information, where the scanning information includes sample information (sample source, etc.).
The lower and/or side portions of the housing 1 are provided with recesses 12 for facilitating gripping by the fingers of a user for ergonomics.
Referring to fig. 6 to 11, the LAMP chip 7 includes a chip body 70, and the chip body 70 is provided with a sample inlet 701, a plurality of reaction chambers 704, and a plurality of exhaust ports 703. Each reaction bin 704 is respectively communicated with the sample adding port 701 through a first micro-channel 705, and each first micro-channel 705 is respectively provided with one or more bending parts. Each reaction chamber 704 is also in communication with a corresponding exhaust 703 via a second microchannel 706. The LAMP chip 7 is a miniaturized microfluidic chip, does not need to be provided with any piston or valve for controlling liquid flow, has small size, and is suitable for being used in a hand-held LAMP detector. The first micro-channel 705 of each reaction chamber 704 is bent at one or more positions to form one or more bending parts, so that reagents and the like in the reaction chamber 704 can be effectively prevented from flowing back out of the first micro-channel 705 without arranging a valve or a piston, and pollution to other reaction chambers 704 is avoided. Amplification reagents such as primers are preset in the reaction chambers 704, and the primers in different reaction chambers 704 can be the same or different to detect different pathogens. The front part of the chip body 70 provided with the sample inlet 701 has a thickness greater than that of the other parts. The chip body 70 as a whole or at least a portion corresponding to the reaction chamber 704 is made of a material having a certain transparency (transparent or translucent) to allow laser light to enter and fluorescence to exit.
The bending part comprises an arc bending part which is arc-shaped integrally, and the central angle of the arc formed by the arc bending part is more than 90 degrees, preferably 170 to 190 degrees. Further, the arc-shaped bending part comprises a first arc-shaped bending part 705a and a second arc-shaped bending part 705b, the circle center of the first arc-shaped bending part 705a is positioned on the right side of the first arc-shaped bending part, and the circle center of the second arc-shaped bending part 705b is positioned on the left side of the second arc-shaped bending part. The central angles of the first arc-shaped bending part 705a and the second arc-shaped bending part 705b are 180 degrees. As shown in fig. 10, the first arc-shaped bending portions 705a and the second arc-shaped bending portions 705b are staggered, and the adjacent first arc-shaped bending portions 705a and second arc-shaped bending portions 705b are directly connected or connected through linear channels, that is, each first micro-channel 705 has a wavy portion, so that reagents such as primers in the reaction bin 704 are effectively prevented from flowing backwards.
In another embodiment, the bending portion includes a plurality of first bending portions and a plurality of second bending portions, and each of the first bending portions integrally forms an acute angle, and each of the second bending portions integrally forms an acute angle. The first bending portions and the second bending portions are staggered, and adjacent first bending portions and second bending portions are directly connected or connected through linear channels, that is, each first micro channel 705 has a portion in a zigzag shape.
The chip body 70 is integrally formed of plastic, such as injection molding, and has a plate shape. The chip body 70 has opposite first and second surfaces 70a and 70b, and in this embodiment, the first surface 70a is specifically an upper surface of the chip body 70, and the second surface 70b is specifically a lower surface of the chip body 70. The sample inlet 701 and the air outlet 703 are provided on the first surface 70a of the chip body 70, and the reaction chamber 704, the first micro-channel 705 and the second micro-channel 706 are provided on the second surface 70b of the chip body 70.
The LAMP chip 7 further includes a cover film 71 provided on the surface of the chip body 70 and covering the gas outlet 703, which allows passage of gas but does not allow passage of liquid. The LAMP chip 7 further comprises a first sealing film 72 for sealing the sample loading port 701 and/or the exhaust port 703, and after the sample loading is completed, the first sealing film 72 is coated on the first surface 70a of the chip body 70 to isolate the sample loading port 701 and the exhaust port 703 from the outside, and then amplification reaction is performed to prevent contamination. The first surface 70a of the chip body 70 is further provided with an overflow groove 702 surrounding the sample loading port 701, so as to avoid polluting the first surface 70a of the chip body 70 during sample loading and avoid affecting the adhesion between the first sealing film 72 and the first surface 70 a.
The LAMP chip 7 further includes a second sealing film 73 for sealing the reaction chamber 704, the first micro-channel 705, and the second micro-channel 706, and the second sealing film 73 is coated on the second surface 70 b. The second surface 70b of the chip body 70 is provided with a plurality of grooves 707, and the grooves 707 are specifically formed on the area of the second surface 70b where the reaction chamber 704, the first micro-channel 705 and the second micro-channel 706 are not formed, and adapt to the shapes and positions of the reaction chamber, the first micro-channel 705 and the second micro-channel 706 to have different shapes, sizes and positions, and avoid the reaction chamber, the first micro-channel 705 and the second micro-channel 706. These grooves 707 serve to facilitate sealing of the second sealing film 73 and the chip body 70, avoid air bubbles generated after film attachment, and also prevent bending deformation of the chip body 70.
When the LAMP chip 7 leaves the factory, the coating film 71 and the second sealing film 73 are attached to the proper positions of the chip body 70 in advance, and isothermal amplification reagents including primers and the like are placed in each reaction tank in advance; after the user has added the nucleic acid sample to be measured, the second sealing film 73 is applied to the sample application port 701 and the cover film 71, and the sample application port 701 and the respective exhaust ports 703 are closed.
The right side of the chip body 70 is provided with a rib or a unfilled corner 708, and correspondingly, the slot 21a of the chip holder 2 is correspondingly provided with a positioning groove matched with the positioning rib or a bulge matched with the unfilled corner 708 so as to avoid reverse insertion of the LAMP chip 7 and perform foolproof design.
The hand-held LAMP detector comprises a controller, wherein the controller is electrically connected with the heating assembly 3, the optical detection device 4, the driving mechanism 5, the battery 61, the touch screen 62, the printer 63, the code scanner 64 and the like, so that the LAMP detector can automatically operate, and nucleic acid detection, result display and the like are realized. Fig. 12 shows a control block diagram of the hand-held LAMP detector, wherein the controller specifically comprises a first control chip 81 and a second control chip 82 which are arranged on a PCB board, and the first control chip 81 is a main control chip; the second control chip 82 is a control chip of the printer 63 and is used for communication with the outside, such as by bluetooth, wifi, 4G or 5G.
The power interface 11 is electrically connected to an external power input port of the first control chip 81, and after the power interface 11 is connected to an external power source, the whole LAMP detector can be powered by the external power source and the battery 61 can be charged. The battery 61 is electrically connected to the first control chip 81, and the battery 61 supplies power to the entire LAMP detector when an external power source is not connected. The first control chip 81 converts a 15V voltage from an external power source or a 12V voltage from the battery 61 and supplies the converted voltage to the second control chip 82, the heating unit 3, the motor 53, the touch panel 62, the scanner 64, the printer 63, and the like. The first control chip 81 is further configured to detect an electric quantity of the battery 61, compare the electric quantity with a set threshold, and perform normal operation of the LAMP detector when the electric quantity is greater than or equal to the set threshold; when the amount of electricity is less than the set threshold, a low electricity alarm signal is generated through the touch screen 62.
The heating film 32 of the heating assembly 3 is electrically connected to the serial port of the first control chip 81 through the fuse 34, and the temperature sensor 33 is electrically connected to the other serial port of the first control chip 81. The first control chip 81 is used for receiving the temperature detection data of the temperature sensor 33, comparing the temperature detection data with the set temperature, and adjusting the temperature of the heating film 32 in real time by adopting a PID algorithm, so that the heating temperature of the heat conducting plate 31 is maintained at 65+/-0.5 ℃.
The motor 53 is electrically connected with the serial port of the first control chip 81, the serial port of the first control chip 81 is also electrically connected with the optocoupler, and whether the motor 53 rotates to a set angle or not is detected through the optocoupler so as to reset and limit the motor 53. The spectrum sensor of the optical detection device 4 is electrically connected to the serial port of the first control chip 81 to receive the detection result. After the motor 53 is at the initial position and the set reaction time is passed, the spectrum sensor analyzes and detects the fluorescence of the first reaction chamber 704 and sends the detection result to the first control chip 81; after the first reaction chamber 704 is detected, the motor 53 rotates to enable the optocoupler sensor to move to the position above the second reaction chamber 704, fluorescence of the second reaction chamber 704 is read, and the read result is sent to the second control chip 82; and so on.
The touch screen 62 is electrically connected with the serial port 3 of the first control chip 81, the touch screen 62 can receive a user instruction and send the user instruction to the first control chip 81, and the first control chip 81 can send detection results, code scanning information and the like to the touch screen 62 for display. The touch screen 62 can also display the alarm signal sent by the first control chip 81, and display the low battery on the screen.
The code scanner 64 is electrically connected with the serial port 2 of the first control chip 81, and the code scanner 64 can read bar code information of a sample to obtain information such as a sample source, a name of a subject and the like; the first control chip 81 can accept the read bar code information and correlate it with the detection result.
The printer 63 is electrically connected with the serial port 0 of the first control chip 81, the other serial port 0 of the first control chip 81 is electrically connected with the serial port 0 of the second control chip 82, and the first control chip 81 can send the read bar code information and the detection result to the printer 63 for printing.
Referring to fig. 13, the control flow of the hand-held LAMP detector is as follows:
s100, the controller obtains the electric quantity of the battery 61
The first control chip 81 detects the electric quantity of the battery 61 and judges whether the electric quantity is greater than or equal to a set threshold value, if yes, the following steps are executed; if not, a low battery alarm signal is generated by the touch screen 62 or the like.
After receiving the low battery alarm signal, the user is connected to an external power supply, and the external power supply supplies power to charge the battery 61.
S101, code scanning
The barcode information is read by the barcode scanner 64, and the barcode information includes sample information, user information, and the like, and the barcode information may be a one-dimensional code or a two-dimensional code provided on the LAMP chip 7.
S102, loading the LAMP chip 7
The LAMP chip 7 was inserted horizontally from the chip port 10 into a hand-held LAMP detector, in which the LAMP chip 7 had been pretreated. The pretreatment process is specifically as follows: adding samples from the sample adding port 701 until the samples flow to the position of the coating film 71 to seal the coating film 71, and even the added samples overflow into the overflow groove 702; the sample inlet 701 and the cover film 71 are covered with a first sealing film 72, and the sample inlet 701 and the respective air outlet 703 are closed, so that a closed environment isolated from the outside air is formed in the LAMP chip 7, and isothermal amplification and fluorescence detection are performed in the closed environment.
S103, the touch screen 62 receives detection instructions of the user
The touch screen 62 is switched to a display state having a "start detection" button, and the user clicks the "start detection" button on the touch screen 62 to start the detection flow.
S104, the controller receives the detection instruction, and controls the motor 53 to reset and controls the heating assembly 3 to heat
After receiving the start detection instruction of the user, the first control chip 81 sends a control signal to the motor 53, and the motor 53 is reset to the start position in response to the control signal; the first control chip 81 specifically determines whether the motor 53 returns to the starting position by the signal returned by the optocoupler. Meanwhile, the first control chip 81 controls the heating film 32 to heat, specifically: the first control chip 81 receives the heating temperature value fed back from the temperature sensor 33, compares the heating temperature value with a set temperature value (65 ℃) and controls the heating temperature of the heating film 32 to be maintained at 65±0.5 ℃ according to the comparison result by a pid algorithm, so that the reaction system in each reaction chamber 704 of the LAMP chip 7 can be heated at a constant temperature.
S105, the controller controls the optical detection device 4 to read the detection result of the first reaction chamber 704
The first control chip 81 sends a control signal to the motor 53, and in response to the control signal, the motor 53 rotates by an angle converted from the initial position of the optical detection device 4 and the pitch of the first reaction chamber 704, so that the optical detection device 4 can be moved to the first detection position opposite to the first reaction chamber 704, the amplified product in the first reaction chamber 704 is irradiated with laser light, the excited fluorescence is collected, the collected fluorescence is analyzed by the spectrum sensor, and the fluorescence analysis value is sent to the first control chip 81.
S106, the controller controls the optical detection device 4 to read the detection result of the next reaction chamber 704
After receiving the fluorescence analysis value of the previous reaction chamber 704, the first control chip 81 sends a control signal to the first control chip 81, so that the motor 53 continues to rotate by another set angle, the angle is obtained by converting the distance between two adjacent reaction chambers 704, and therefore the optical detection device 4 can be moved to the next detection position opposite to the next reaction chamber 704 (such as the second reaction chamber 704, the third reaction chamber 704, the fourth reaction chamber 704 and the like), so as to perform laser irradiation on the amplification product in the next reaction chamber 704, collect the excited fluorescence, analyze the collected fluorescence through a spectrum sensor, and send the fluorescence analysis value to the first control chip 81.
S107, the controller judges the fluorescence analysis value sent by the optical detection device 4 to obtain the detection result of the detection item corresponding to each reaction bin 704
After receiving the fluorescence analysis value returned by the optical detection device 4 for the first time, the first control chip 81 judges the yin-yang of the detection item (such as pathogen a) corresponding to the first reaction chamber 704 according to the pre-stored interpretation standard. After receiving the fluorescence analysis value returned by the optical detection device 4 for the second time, the first control chip 81 judges the yin-yang of the detection item (such as pathogen B) corresponding to the second reaction chamber 704 according to the pre-stored judging standard. After receiving the fluorescence analysis value returned by the optical detection device 4 for the third time, the first control chip 81 judges the yin-yang of the detection item (such as pathogen C) corresponding to the third reaction chamber 704 according to the pre-stored interpretation standard. After receiving the fluorescence analysis value returned by the optical detection device 4 for the fourth time, the first control chip 81 judges the yin-yang of the detection item (such as pathogen D) corresponding to the fourth reaction chamber 704 according to the pre-stored interpretation standard. And so on.
S108, the controller sends the detection result to the printer 63 for printing and sends the detection result to the touch screen 62 for display
The first control chip 81 transmits the detection result (negative/positive) of each detection item and the information read by the scan code to the printer 63, and the second control chip 82 controls the printer 63 to print out a detection report. Meanwhile, the first control chip 81 transmits the detection result of each detection item to the touch screen 62, and the touch screen 62 is switched to another display state, and the detection result and the information read by the scan code are displayed.
The LAMP detector and the detection system have the advantages that the layout and the configuration are adopted for all internal components, so that the whole LAMP detector is compact in structure and small in size (the whole LAMP detector can be 250mm multiplied by 120mm multiplied by 80 mm), is powered by the battery 61, can be used for handheld detection on the premise of integrating nucleic acid amplification and detection, automatic code scanning, detection result display and printing, and is convenient to carry and use; the user can automatically finish code scanning, constant-temperature heating amplification, fluorescence detection analysis and detection result display printing by loading the LAMP chip 7 after sample addition, so that the detection efficiency is improved. The adopted LAMP chip 7 can keep the reagents of each reaction tank not to interfere with each other under the condition of not configuring a control valve or a piston, so that pollution is avoided; only one sample adding port 701 is needed to be added with a sample, so that the use is convenient; because no control valve or piston is arranged, the overall volume of the LAMP chip 7 is smaller (can be as small as 34mm multiplied by 58mm multiplied by 3 mm), so that the overall volume of the LAMP detector which is matched with the LAMP chip is greatly reduced, and the LAMP chip is suitable for handheld detection.
As used in this specification and in the claims, the terms "comprises" and "comprising" merely indicate that the steps and elements are explicitly identified, and do not constitute an exclusive list, as other steps or elements may be included in a method or apparatus. The term "and/or" as used herein includes any combination of one or more of the associated listed items.
It should be noted that, unless otherwise specified, when a feature is referred to as being "fixed" or "connected" to another feature, it may be directly or indirectly fixed or connected to the other feature. Further, the descriptions of the upper, lower, left, right, etc. used in the present invention are merely with respect to the mutual positional relationship of the constituent elements of the present invention in the drawings.
It is further understood that the terms "first," "second," and the like are used to describe various information, but such information should not be limited to these terms. These terms are only used to distinguish one type of information from another and do not denote a particular order or importance. Indeed, the expressions "first", "second", etc. may be used entirely interchangeably. For example, first information may also be referred to as second information, and similarly, second information may also be referred to as first information, without departing from the scope of the present disclosure.
The above-described embodiments are provided for illustrating the technical concept and features of the present invention, and are intended to be preferred embodiments for those skilled in the art to understand the present invention and implement the same according to the present invention, not to limit the scope of the present invention. All equivalent changes or modifications made according to the principles of the present invention should be construed to be included within the scope of the present invention.
Claims (10)
1. The LAMP detector is characterized by comprising a chip holder for accommodating an LAMP chip, a heating component for heating the LAMP chip at constant temperature, an optical detection device capable of moving in the left-right direction, a driving mechanism for driving the optical detection device to move in the left-right direction, a touch screen serving as a human-computer interaction interface, a battery for supplying power and a controller,
the controller is used for detecting the electric quantity of the battery, comparing the electric quantity with a set threshold value and normally operating when the electric quantity is greater than or equal to the set threshold value;
the touch screen is provided with a plurality of display states, and in one display state, the touch screen is provided with an area for a user to input instructions, and the touch screen is used for receiving a start detection instruction of the user in the display state and sending the start detection instruction to the controller; the controller is also used for sending a control signal for resetting the optical detection device to a starting position and a control signal for heating the heating component at constant temperature to the driving mechanism after receiving a start detection instruction sent by the touch screen;
the controller is further used for sending a control signal for enabling the optical detection device to move to a certain reaction bin corresponding to the LAMP chip to the driving mechanism; the optical detection device is used for carrying out laser irradiation and fluorescence collection and analysis on the opposite reaction bin and sending a fluorescence analysis value to the controller; the controller is also used for judging the negative and positive of the detection item corresponding to the reaction bin according to the fluorescence analysis value sent by the optical detection device and the prestored interpretation standard;
the controller is also used for sending the interpreted detection result to the touch screen for display;
the chip holder comprises a fixing part and a supporting part positioned at the rear side of the fixing part, the accommodating space comprises a slot which is arranged on the fixing part and used for the LAMP chip to penetrate in, the slot penetrates through the fixing part along the front-rear direction, the slot is configured in such a way that the rear part of the LAMP chip penetrates out of the slot to face the optical detection device after the LAMP chip is inserted in, and the supporting part is provided with a supporting surface which is used for facing the rear part of the LAMP chip;
the heating component is arranged on the supporting surface of the chip seat and comprises a heat conducting plate and a pressing elastic piece, the heat conducting plate is arranged on the supporting surface, the pressing elastic piece is used for pressing the LAMP chip on the heat conducting plate, the pressing elastic piece is fixedly arranged on the fixing seat and can elastically deform, and the pressing elastic piece is configured to be bent and deformed after the LAMP chip is inserted so as to press the LAMP chip on the heat conducting plate;
the LAMP chip is configured to have a plurality of reaction bins arranged side by side in the left-right direction, and the plurality of reaction bins are arranged on the rear part of the LAMP chip which passes out of the slot.
2. The LAMP detector of claim 1, further comprising a printer, wherein the controller is further configured to send the interpreted detection result to the printer for printing.
3. The LAMP detector of claim 1, further comprising a code scanner, wherein the controller is further configured to receive barcode information read by the code scanner and correlate the barcode information with the detection result.
4. The LAMP detector of claim 1, wherein the heating assembly further comprises a heating film, a thermal insulation cotton, a temperature sensor and a fuse, the thermal insulation cotton is arranged on the chip holder, the heating film is arranged on the thermal insulation cotton, the heat conducting plate is arranged on the heating film, the temperature sensor is electrically connected with the controller, the heating film is electrically connected with the controller through the fuse, and the controller is further used for receiving a heating temperature value returned by the temperature sensor, comparing the heating temperature value with a set temperature value and controlling the heating temperature of the heating film according to a comparison result.
5. The LAMP detector of claim 1, wherein the driving mechanism comprises a guide rail extending in a left-right direction, a mounting block movably arranged on the guide rail in the left-right direction, and a motor for driving the mounting block to move in the left-right direction, the optical detection device is arranged on the mounting block, the motor is electrically connected with the controller, and the motor is used for responding a control signal sent by the controller to the driving mechanism to rotate by a corresponding angle.
6. The LAMP detector of claim 1, wherein the battery is a rechargeable battery, the LAMP detector comprising a power interface for accessing an external power source, the power interface being electrically connected to the controller.
7. The LAMP detector of claim 1, wherein the controller is configured to issue an alarm signal when the power level is less than the set threshold.
8. The control method of the LAMP detector as claimed in any one of claims 1 to 7, comprising the steps of:
A. acquiring the electric quantity of the battery, judging whether the electric quantity is larger than or equal to a set threshold value, and if so, executing the following steps;
B. the method comprises the steps of scanning a code to read bar code information, wherein the bar code information comprises sample information and/or user information;
C. receiving a detection instruction of a user through a touch screen;
D. the driving mechanism resets the optical detection device and heats the heating component at constant temperature;
E. the driving mechanism enables the optical detection device to move to the first reaction bin and reads the detection result of the first reaction bin;
F. the driving mechanism enables the optical detection device to move to the next reaction bin and reads the detection result of the next reaction bin;
repeating the step F until all the reaction bins are read;
G. judging the fluorescence analysis value sent by the optical detection device to obtain the detection result of the detection item corresponding to each reaction bin;
H. and sending the detection result to the touch screen for display.
9. The control method according to claim 8, wherein in step a, if no, a low power alarm signal is generated by the touch screen; and/or, the LAMP detector is powered by an external power supply and charges a battery.
10. The control method according to claim 8, wherein in step H, the detection result is also sent to a printer for printing.
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| CN202111209767.1A CN113930322B (en) | 2021-10-12 | 2021-10-12 | LAMP detector and control method |
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| CN202111209767.1A CN113930322B (en) | 2021-10-12 | 2021-10-12 | LAMP detector and control method |
| CN202111187960.XA CN113773952A (en) | 2021-10-12 | 2021-10-12 | Handheld LAMP detector and system |
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| CN202111187960.XA Division CN113773952A (en) | 2021-10-12 | 2021-10-12 | Handheld LAMP detector and system |
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| CN113930322A CN113930322A (en) | 2022-01-14 |
| CN113930322B true CN113930322B (en) | 2024-03-08 |
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| CN202111209767.1A Active CN113930322B (en) | 2021-10-12 | 2021-10-12 | LAMP detector and control method |
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| WO (1) | WO2023060851A1 (en) |
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| WO2023050711A1 (en) * | 2021-09-28 | 2023-04-06 | 江苏汇先医药技术有限公司 | Multi-channel lamp detector |
| CN216274121U (en) * | 2021-10-12 | 2022-04-12 | 江苏汇先医药技术有限公司 | Handheld LAMP detector and system |
| CN219117443U (en) * | 2021-10-12 | 2023-06-02 | 上海邦先医疗科技有限公司 | LAMP chip |
| CN113773952A (en) * | 2021-10-12 | 2021-12-10 | 江苏汇先医药技术有限公司 | Handheld LAMP detector and system |
| CN117808025A (en) * | 2024-02-29 | 2024-04-02 | 宁德时代新能源科技股份有限公司 | Method for checking battery cell outbound and battery cell outbound system |
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- 2021-10-12 CN CN202111187960.XA patent/CN113773952A/en active Pending
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| CN113773952A (en) | 2021-12-10 |
| WO2023060851A1 (en) | 2023-04-20 |
| CN113930322A (en) | 2022-01-14 |
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