CN213506974U - Portable real-time fluorescent quantitative PCR instrument - Google Patents
Portable real-time fluorescent quantitative PCR instrument Download PDFInfo
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- CN213506974U CN213506974U CN202021556305.8U CN202021556305U CN213506974U CN 213506974 U CN213506974 U CN 213506974U CN 202021556305 U CN202021556305 U CN 202021556305U CN 213506974 U CN213506974 U CN 213506974U
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Abstract
The utility model relates to a portable real-time fluorescence quantitative PCR instrument, which comprises an installation bottom plate, and a circuit control module, a temperature control module, a driving motor, a transmission device and an optical detection module which are arranged on the installation bottom plate; the circuit control module is respectively connected with the control temperature control module, the driving motor and the optical detection module and is used for controlling the driving motor and the optical detection module to scan the fluorescence signals of the EP tubes, collecting fluorescence signal data and uploading the fluorescence signal data to an upper computer and controlling the temperature control module to provide a corresponding temperature environment for PCR; the temperature control module is used for providing ambient temperature for the EP pipe arranged on the temperature control module; the output shaft of the driving motor is connected with the transmission device; the transmission device is arranged on one side of the temperature control module; the optical detection module is arranged on the transmission device, and the transmission device drives the optical detection module to perform linear scanning motion so as to perform scanning detection on the side wall of the reaction system in the EP tube. The utility model discloses a small, it is fast to go up and down the temperature rate, uses portablely.
Description
Technical Field
The utility model relates to a fluorescence detection instrument, in particular to a portable real-time fluorescence quantitative PCR instrument applied in the biological and medical detection field.
Background
Polymerase Chain Reaction (PCR) has been widely used in the biological and medical fields as a commonly used analytical means in molecular biology. At present, most real-time fluorescence quantitative PCR instruments on the market are in a standard 96-well plate mode, are large and heavy, need to be placed in a research room or a clinical laboratory, and are not suitable for environments requiring portability, such as rapid detection, fields and the like. In addition, most commercially available fluorescence quantitative PCR instruments adopt an imaging optical detection mode, and multiple light paths share the same excitation light source and the same imaging device, so that the optical path difference is formed, and the edge effect of fluorescence detection is formed; some scanning detection modes adopting the EP tube cover or the tube bottom are adopted, and the tube cover or the tube bottom which is used as a scanning structure is easily influenced by the temperature of the hot cover and the temperature control module, so that the reading of a detection device can be interfered.
In view of the above, there is a need to develop a real-time fluorescence PCR detection apparatus that is portable, suitable for field operation, fast in temperature increase and decrease rate, and accurate and not prone to be interfered in optical detection.
Disclosure of Invention
To the above problem, the utility model aims at providing a portable real-time fluorescence quantitative PCR appearance, it can realize field operation, on-vehicle charging, simple structure, convenient operation, small in size.
In order to achieve the purpose, the utility model adopts the following technical proposal: a portable real-time fluorescence quantitative PCR instrument comprises an installation bottom plate, and a circuit control module, a temperature control module, a driving motor, a transmission device and an optical detection module which are arranged on the installation bottom plate; the circuit control module is respectively connected with the temperature control module, the driving motor and the optical detection module and is used for controlling the driving motor and the optical detection module to scan the fluorescence signals of the EP tubes and collecting fluorescence signal data to be uploaded to an upper computer; and controlling the temperature control module to provide a corresponding temperature environment for PCR; the temperature control module is used for providing ambient temperature for the EP pipe arranged on the temperature control module; the output shaft of the driving motor is connected with the transmission device; the transmission device is arranged on one side of the temperature control module; the optical detection module is arranged on the transmission device, and the transmission device drives the optical detection module to perform linear scanning motion so as to perform side wall scanning detection on the EP pipe.
Further, the temperature control module comprises a temperature control body, an EP tube adapter, a temperature sensor, a peltier and a heat sink; the temperature control body is arranged on the mounting bottom plate; the EP pipe adapter pieces are arranged along the length direction of the temperature control body at intervals and used for placing the EP pipes; the temperature sensor is arranged at the bottom of the EP pipe adapter and transmits the detected temperature to the circuit control module, and the circuit control module adjusts the Peltier according to the temperature fed back by the temperature sensor; the Peltier is arranged at the bottom of the EP pipe adapter and provides a heating and cooling power source for the EP pipe according to the control signal transmitted by the circuit control module; the heat dissipation device is arranged at the bottom of the EP pipe adapter and used for dissipating heat of the Peltier.
Furthermore, a plurality of detection holes are formed in the side wall of the front end of the temperature control body at intervals.
Further, the inspection hole is provided corresponding to the EP pipe adapter.
Further, the heat dissipation device adopts an air-cooled heat dissipation structure or a water-cooled heat dissipation structure.
Furthermore, the optical detection module is provided with more than one optical detection channel, each optical detection channel comprises an excitation light source, and excitation light emitted by the excitation light source sequentially passes through an excitation light filter, a collimating lens, a dichroic mirror and a converging lens and then is emitted to the side wall of the EP tube through the detection hole to excite a reactant in the EP tube to emit fluorescence; the excited fluorescence returns to the convergent lens through the detection hole, enters the dichroic mirror after being collimated by the convergent lens, penetrates through the dichroic mirror, and then enters the detector through the transmitting end convergent lens and the transmitting light filter in sequence to be collected.
Further, the motion trail of the optical detection module is parallel to the axis of the detection hole of each EP tube.
Further, the transmission device adopts a belt transmission structure or a lead screw transmission structure.
Further, the installation base plate comprises a shell which covers the upper part of the installation base plate; the shell is provided with a hot cover, the hot cover is positioned above the temperature control module, is connected with the shell through a connecting shaft, and is controlled by the circuit control module to control the temperature of the hot cover.
Further, a control display module is arranged on the shell; and the control display module is connected with the circuit control module and used for carrying out experimental setting, displaying and outputting a detection result.
The utility model discloses owing to take above technical scheme, it has following advantage: 1. the utility model discloses simple structure, convenient operation, the volume is less, portable. 2. The utility model discloses an optical channel is independently difficult for receiving the interference, and the detection data is accurate.
Drawings
Fig. 1 is a schematic view of the whole structure of the present invention without the outer casing.
Fig. 2 is a schematic view of the overall structure of the belt housing of the present invention.
Fig. 3 is a schematic structural diagram of the temperature control module and the optical scanning device of the present invention.
Fig. 4 is a schematic structural diagram of the optical detection module of the present invention.
Reference numerals: 1, mounting a bottom plate; 2-a circuit control module; 3-temperature control module, 31-temperature control body, 32-EP tube, 33-detection hole; 4-driving the motor; 5-optical detection module, 51-excitation light source, 52-excitation light filter, 53-collimating lens, 54-dichroic mirror, 55-converging lens, 56-emission end converging lens, 57-emission light filter, 58-detector; 6-a water tank; 70-driving wheel, 71-driven wheel, 72-belt, 73-fixed seat and 74-guide rail; 8-shell, 81-hot cover, 82-liquid crystal control display module, 83-key and indicator lamp, 84-liquid injection hole, 85-connecting shaft; 9-backboard, 91-switch.
Detailed Description
In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the drawings of the embodiments of the present invention are combined below to clearly and completely describe the technical solution of the embodiments of the present invention. It is to be understood that the embodiments described are only some of the embodiments of the present invention, and not all of them. All other embodiments, which can be derived from the description of the embodiments of the present invention by a person skilled in the art, are within the scope of the present invention.
In the description of the present invention, it should be understood that the terms "upper", "lower", "inner", "outer", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention.
As shown in fig. 1-3, the utility model provides a portable real-time fluorescence quantitative PCR instrument, which comprises a mounting base plate 1, and a circuit control module 2, a temperature control module 3, a driving motor 4, a transmission device and an optical detection module 5 which are arranged on the mounting base plate 1. Wherein:
the circuit control module 2 is respectively connected with the temperature control module 3, the driving motor 4 and the optical detection module 5, and is used for controlling the driving motor 4 and the optical detection module 5 to scan the fluorescence signals of the EP tubes, collecting fluorescence signal data and uploading the fluorescence signal data to an upper computer; meanwhile, the temperature control module 3 is also controlled to provide a corresponding temperature environment for PCR;
the temperature control module 3 is used for providing ambient temperature for the EP pipe arranged on the temperature control module;
the output shaft of the driving motor 4 is connected with the transmission device;
the transmission device is arranged on one side of the temperature control module 3;
the optical detection module 5 is arranged on the transmission device, the transmission device drives the optical detection module 5 to perform linear scanning movement, and the EP tube on the temperature control module 3 is subjected to side wall scanning detection so as to obtain a fluorescence signal value of a reactant in the EP tube on the temperature control module 3.
In a preferred implementation, the temperature control module 3 can provide a temperature environment of 4-99 degrees. The temperature control module 3 comprises a temperature control body 31, an EP tube adapter, a temperature sensor, peltier and a heat sink. Wherein:
a temperature control body 31 mounted on the mounting base plate 1;
a plurality of EP tube adapters arranged at intervals along the length direction of the temperature control body 31 for placing EP tubes 32; in this embodiment, 16 EP tube adapters are preferably provided, allowing for simultaneous real-time fluorescent quantitative PCR reaction detection of 2 octal EP tubes 32;
the temperature sensor is arranged at the bottom of the EP pipe adapter and transmits the detected temperature to the circuit control module 2, and the circuit control module 2 adjusts the Peltier according to the temperature fed back by the temperature sensor so as to control the temperature of the EP pipe 32;
the Peltier is arranged at the bottom of the EP pipe adapter and provides a heating and cooling power source for the EP pipe 32 according to the control signal transmitted by the circuit control module 2;
and the heat dissipation device is arranged at the bottom of the EP pipe adapter and used for dissipating heat for the Peltier. In this embodiment, the heat dissipation device may adopt an air-cooling heat dissipation structure or a water-cooling heat dissipation structure.
In the above embodiment, when the heat sink adopts a water-cooling heat dissipation structure, the mounting base plate 1 is further provided with the water tank 6. The outlet of the water tank 6 is connected with the heat dissipation device through a pipeline, and the cooling speed can be faster through cold water in the water tank 6, and the noise is smaller.
In the above embodiment, a plurality of detection holes 33 are provided at intervals on the side wall of the front end of the temperature control body 31, the detection holes 33 are provided corresponding to the EP tube adapter, and the reactant in the EP tube 32 placed in the EP tube adapter is detected through the detection holes 33.
In a preferred embodiment, the transmission device may adopt a belt transmission structure, a screw transmission structure, or the like, which is not limited herein.
When the belt transmission structure is adopted, as shown in fig. 3, the transmission device includes a driving wheel 70, a driven wheel 71, a belt 72, a fixed seat 73, and a guide rail 74. Wherein:
a driving wheel 70 coaxially connected with the output shaft of the driving motor 4 and driven by the driving motor 4 to rotate;
the driven wheel 71 is in transmission connection with the driving wheel 70 through a belt 72;
a fixing base 73, the lower part of which is arranged on the belt 72, and the optical detection module 5 is fixed on the fixing base 73;
and the guide rail 74 is arranged at the front end of the temperature control module 3 and is installed between the two fixing frames 33, and the upper part of the fixing seat 73 is installed in a matching way with the guide rail 74, so that the optical detection module 5 can perform one-dimensional linear motion along the guide rail 74.
In a preferred embodiment, as shown in fig. 4, the optical detection module 5 is provided with more than one optical detection channels, each optical detection channel includes an excitation light source 51, the excitation light emitted from the excitation light source 51 sequentially passes through an excitation light filter 52, a collimating lens 53, a dichroic mirror 54 and a converging lens 55, and then passes through the detection hole 33 to reach the side wall of the EP tube 32, so as to excite the reactant in the EP tube 32 to emit fluorescence; the excited fluorescence returns to the converging lens 55 through the detection hole 33, is collimated by the converging lens 55, enters the dichroic mirror 54, passes through the dichroic mirror 54, and then enters the detector 58 through the emission end converging lens 56 and the emission light filter 57 in sequence to be collected. In this embodiment, it is preferable to provide 4 optical detection channels, where the 4 optical detection channels have excitation light sources with different spectra, filter sets and detection devices to form independent optical paths, and the 4 optical paths are arranged in parallel along the scanning direction.
In the present embodiment, the motion trajectory of the optical detection module 5 is parallel to the axis of the detection hole 33 of each EP tube 32 by using a side wall scanning method.
In a preferred embodiment, the portable real-time fluorescence quantitative PCR instrument of the present invention further comprises a housing 8 covering the upper portion of the mounting base plate 1. As shown in fig. 2, the case 8 is provided with a heat cover 81, a control display module 82, a key and indicator lamp 83, and an injection hole 84. Wherein:
the thermal cover 81 is positioned above the temperature control module 3, is connected with the shell 8 through a connecting shaft 85, and controls the temperature of the thermal cover 81 through the circuit control module 2; the thermal cover 81 and the temperature control module 3 together provide a temperature environment required by PCR; in this embodiment, the thermal cover 81 can provide a high temperature of about 100 degrees, so as to prevent the PCR system in the EP tube from vaporizing and forming condensation on the top cover due to the temperature change of the PCR process provided by the temperature control module 3 at the bottom of the EP tube.
The control display module 82 is connected with the circuit control module 2 and used for carrying out experimental setting on the PCR instrument, displaying and outputting a detection result;
a key and indicator lamp 83 for opening the thermal cover 81 and displaying the working state of the whole instrument by the color of the indicator lamp;
and a liquid inlet 84 connected to an inlet of the water tank 6 for injecting cold water into the water tank 6.
In a preferred implementation, a back plate 9 is further connected to one side of the installation base plate 1, a switch 91 and a power supply are arranged on the back plate 9, the power supply is used for supplying power to the circuit control module 2, the temperature control module 3, the optical detection module 5 and the driving motor 4, and the working state of the power supply is controlled by the switch 91.
To sum up, the utility model discloses a portable real-time fluorescence quantitative PCR appearance operation flow as follows:
in the power-on state, the hot cover 81 is opened through a key, the EP tube 32 is placed in the EP tube adapter, and the hot cover 81 is covered; the PCR reaction process is set by controlling the display module 82, the temperature control module 3 works after the experiment is started, the circuit control module 2 controls the temperature module 3 to provide the temperature required by the PCR reaction in each step, meanwhile, the optical detection module 5 is controlled by the circuit control module 2 to scan the reactants in each EP tube 32 in sequence, and fluorescence signal data are collected and uploaded to an upper computer for further signal storage; and (4) analyzing by an upper computer, then drawing a real-time fluorescence curve, and displaying and subsequently analyzing a result after an experiment.
Those of ordinary skill in the art will understand that: the discussion of any embodiment above is meant to be exemplary only, and is not intended to intimate that the scope of the disclosure, including the claims, is limited to these examples; within the idea of the invention, also combinations between technical features in the above embodiments or in different embodiments are possible, and there are many other variations of the different aspects of the invention as described above, which are not provided in detail for the sake of brevity. Therefore, any omission, modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included within the protection scope of the present invention.
Claims (10)
1. A portable real-time fluorescence quantitative PCR instrument is characterized by comprising: the device comprises a mounting bottom plate, and a circuit control module, a temperature control module, a driving motor, a transmission device and an optical detection module which are arranged on the mounting bottom plate;
the circuit control module is respectively connected with the temperature control module, the driving motor and the optical detection module and is used for controlling the driving motor and the optical detection module to scan the fluorescence signals of the EP tubes and collecting fluorescence signal data to be uploaded to an upper computer; and controlling the temperature control module to provide a corresponding temperature environment for PCR;
the temperature control module is used for providing ambient temperature for the EP pipe arranged on the temperature control module;
the output shaft of the driving motor is connected with the transmission device;
the transmission device is arranged on one side of the temperature control module;
the optical detection module is arranged on the transmission device, and the transmission device drives the optical detection module to perform linear scanning motion so as to perform side wall scanning detection on the EP pipe.
2. The portable real-time fluorescent quantitative PCR instrument as set forth in claim 1, wherein: the temperature control module comprises a temperature control body, an EP pipe adapter, a temperature sensor, a Peltier and a heat dissipation device;
the temperature control body is arranged on the mounting bottom plate;
the EP pipe adapter pieces are arranged along the length direction of the temperature control body at intervals and used for placing the EP pipes;
the temperature sensor is arranged at the bottom of the EP pipe adapter and transmits the detected temperature to the circuit control module, and the circuit control module adjusts the Peltier according to the temperature fed back by the temperature sensor;
the Peltier is arranged at the bottom of the EP pipe adapter and provides a heating and cooling power source for the EP pipe according to the control signal transmitted by the circuit control module;
the heat dissipation device is arranged at the bottom of the EP pipe adapter and used for dissipating heat of the Peltier.
3. The portable real-time fluorescent quantitative PCR instrument as set forth in claim 2, wherein: a plurality of detection holes are formed in the side wall of the front end of the temperature control body at intervals.
4. The portable real-time fluorescent quantitative PCR instrument as set forth in claim 3, wherein: the inspection hole is provided corresponding to the EP pipe adapter.
5. The portable real-time fluorescent quantitative PCR instrument as set forth in claim 2, wherein: the heat dissipation device adopts an air-cooling heat dissipation structure or a water-cooling heat dissipation structure.
6. The portable real-time fluorescent quantitative PCR instrument as set forth in claim 3, wherein: the optical detection module is provided with more than one optical detection channel, each optical detection channel comprises an excitation light source, and excitation light emitted by the excitation light source sequentially passes through an excitation light filter, a collimating lens, a dichroic mirror and a converging lens and then is emitted to the side wall of the EP tube through the detection hole to excite a reactant in the EP tube to emit fluorescence; the excited fluorescence returns to the convergent lens through the detection hole, enters the dichroic mirror after being collimated by the convergent lens, penetrates through the dichroic mirror, and then enters the detector through the transmitting end convergent lens and the transmitting light filter in sequence to be collected.
7. The portable real-time fluorescent quantitative PCR instrument as set forth in claim 6, wherein: the movement locus of the optical detection module is parallel to the axis of the detection hole of each EP tube.
8. The portable real-time fluorescent quantitative PCR instrument as set forth in claim 1, wherein: the transmission device adopts a belt transmission structure or a lead screw transmission structure.
9. The portable real-time fluorescent quantitative PCR instrument as set forth in any one of claims 1 to 8, wherein: the mounting base plate is provided with a mounting base plate; the shell is provided with a hot cover, the hot cover is positioned above the temperature control module, is connected with the shell through a connecting shaft, and is controlled by the circuit control module to control the temperature of the hot cover.
10. The portable real-time fluorescent quantitative PCR instrument as set forth in claim 9, wherein: the shell is provided with a control display module; and the control display module is connected with the circuit control module and used for carrying out experimental setting, displaying and outputting a detection result.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202021556305.8U CN213506974U (en) | 2020-07-31 | 2020-07-31 | Portable real-time fluorescent quantitative PCR instrument |
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| CN202021556305.8U CN213506974U (en) | 2020-07-31 | 2020-07-31 | Portable real-time fluorescent quantitative PCR instrument |
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| CN213506974U true CN213506974U (en) | 2021-06-22 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023178710A1 (en) * | 2022-03-23 | 2023-09-28 | 无锡百泰克生物技术有限公司 | Portable ultrafast real-time fluorescent quantitative pcr instrument |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023178710A1 (en) * | 2022-03-23 | 2023-09-28 | 无锡百泰克生物技术有限公司 | Portable ultrafast real-time fluorescent quantitative pcr instrument |
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