CN217351365U - Portable ultra-fast real-time fluorescence quantitative PCR instrument - Google Patents
Portable ultra-fast real-time fluorescence quantitative PCR instrument Download PDFInfo
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- CN217351365U CN217351365U CN202220646804.9U CN202220646804U CN217351365U CN 217351365 U CN217351365 U CN 217351365U CN 202220646804 U CN202220646804 U CN 202220646804U CN 217351365 U CN217351365 U CN 217351365U
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/02—Apparatus for enzymology or microbiology with agitation means; with heat exchange means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
- C12M1/38—Temperature-responsive control
Abstract
The utility model discloses a portable ultrafast real-time fluorescence quantitative PCR appearance, include: the chip mounting module is used for mounting the PCR reaction chip; the temperature control floating module comprises a temperature control device, the end part of the temperature control floating module is provided with a heating and refrigerating sheet and a temperature sensor, the heating and refrigerating sheet is provided with a heating and refrigerating carrier plate, two sides of the heating and refrigerating carrier plate are provided with heat preservation pressing plates, and the chip mounting module is pressed through the heat preservation pressing plates so that the PCR reaction chip is arranged at the heating and refrigerating carrier plate; and the optical module comprises a switching device and an in-position sensor. The utility model is convenient to carry and transfer; the single chip is adopted, the operation is simple, the sealing is reliable, the detection result is rapid, stable and reliable, the method is suitable for various different detection targets, and the applicability is wider.
Description
Technical Field
The utility model belongs to the technical field of the biological detection technique and specifically relates to a portable ultrafast real-time fluorescence quantitative PCR appearance is related to.
Background
The PCR instrument, also known as a PCR gene amplification instrument, a PCR nucleic acid amplification instrument, or a PCR nucleic acid amplification instrument, is an instrument and device for amplifying specific DNA by using a PCR (Polymerase chain reaction) technique, and is widely used in the fields of life science research, biochemical analysis, clinical diagnosis, pharmaceutical analysis, forensic identification, and rapid examination of epidemic situations.
However, the mainstream multichannel real-time fluorescence quantitative PCR instrument in the market has the disadvantages of large volume, heavy weight, complex optical system design, slow detection speed, off-site detection, amplification reaction time of 2-4 hours, on-site confirmation by detection personnel and complex operation. If the time for sampling, sample transportation and queuing is calculated, the required time is longer, which greatly affects the detection efficiency and can cause delay of confirmed diagnosis, thereby bringing greater difficulty to the tracking of the dynamics of patients and being difficult to realize on-site instant detection. The portable multi-channel PCR instrument with simple operation, small volume and short detection time is needed at present, can carry out rapid detection anytime and anywhere, and is also suitable for family or personal purchase to carry out family self-detection.
SUMMERY OF THE UTILITY MODEL
Utility model purpose: in order to overcome the defects of the background art, the utility model discloses a portable ultra-fast real-time fluorescence quantitative PCR instrument.
The technical scheme is as follows: the utility model discloses a portable ultrafast real-time fluorescence quantitative PCR appearance, include:
the PCR reaction chip comprises a chip substrate, wherein a reaction groove is formed in the chip substrate, a bottom film is attached to the surface of the reaction groove, and a reaction channel is formed by the reaction groove and the bottom film;
the chip mounting module comprises a chip mounting bottom plate, wherein a guide slot is arranged on the mounting bottom plate, and the PCR reaction chip is arranged in the guide slot;
the temperature control floating module comprises a temperature control device, wherein a heating and refrigerating plate and a temperature sensor are arranged at the end part of the temperature control device, a heating and refrigerating carrier plate corresponding to the PCR reaction chip is arranged on the heating and refrigerating plate, heat preservation pressing plates are arranged on two sides of the heating and refrigerating carrier plate, and the heating and refrigerating carrier plate is pressed on the temperature control device through the heat preservation pressing plates;
an optical module including a switching device and an in-place sensor; the back of the chip mounting base plate is provided with a through hole which is used as a part of a light path for the excitation light and the fluorescence to pass through.
Furthermore, be equipped with the tube-shape on the chip base member and add the sample pore, tube-shape adds the one end intercommunication formation application of sample channel of sample pore bottom trompil, tube-shape adds the one end intercommunication formation application of sample channel of sample pore wall upper end trompil and forms the passageway of giving vent to anger through pore wall extension and with the other end intercommunication of reaction channel, the tube-shape adds the sample pore and seals through application of sample handhole door threaded connection, application of sample handhole door corresponds tube-shape and adds the sample pore position and is equipped with the extrusion head, along with application of sample handhole door is rotatory, and the extrusion head extrudes the sample into reaction channel, and interior easy operation does not need complicated liquid way drive part.
The extrusion head can extrude the gas in application of sample passageway and the reaction tank by the passageway of giving vent to anger when extrusion sample, and after the tube-shape application of sample hole totally closed, sample evenly distributed was in the reaction intracavity, and application of sample passageway and the outer tip hole of the passageway of giving vent to anger all are sealed by the application of sample handhole door this moment, prevent that reagent from spilling over at the in-process that rises and falls the temperature repeatedly and leading to aerosol to pollute, and sealing performance is reliable stable.
Furthermore, positioning grooves are formed in two sides of the PCR reaction chip, positioning bumps are arranged on the guide slots corresponding to the positioning grooves, and a first in-place sensor is arranged.
Furthermore, the chip installation module further comprises a chip sliding bottom plate, a chip guide rail and a second in-place sensor are arranged on the chip sliding bottom plate, the driving end is connected with the chip installation bottom plate, the chip is controlled to move along the guide rail, and the position is identified through the second in-place sensor.
Furthermore, the temperature control device is arranged on the temperature control main support through the L-shaped fixing frame, the temperature control device is connected with the L-shaped fixing frame through the first temperature control guide rail, meanwhile, the end part of the temperature control device is connected through the pre-tightening spring, extrusion buffering is achieved, the L-shaped fixing frame is arranged on the temperature control main support through the second temperature control guide rail, the temperature control device is driven to move towards the PCR reaction chip through the second motor, and a third in-place sensor is arranged.
Furthermore, the optical path of the optical module generates exciting light by an excitation light source, stray light is filtered by an optical filter and collimated by a lens, the stray light is reflected to the chip reaction tank by the secondary spectroscope, and the generated fluorescent light is received by a fluorescent light detection device through the secondary spectroscope, wherein the fluorescent light detection device is a photodiode or a camera or a PMT.
Through the arrangement and combination of the optical filter, the lens and the secondary spectroscope, the fluorescence detection efficiency is improved on the basis of simplifying the light path and reducing the volume, and the fluorescence detection device can select a photodiode, a camera and a PMT according to different requirements.
Has the advantages that: compared with the prior art, the utility model has the advantages that: the volume is small, the weight is light, and the carrying and the transfer are convenient; the method is simple to operate, has extremely low requirements on users and hardware facilities, has good applicability, and can simultaneously meet the detection requirements of central laboratories, basic medical institutions and family self-inspection; the method is rapid, stable and reliable, can be suitable for various different detection targets, and has wider applicability; the unique design of the rotary cover type chip can effectively seal and prevent aerosol from cross contamination; the temperature is increased and decreased very fast, the inspection time is greatly shortened (15-20min), and the rapid detection is convenient.
Drawings
FIG. 1 is a schematic view of an overall structure of the present invention;
FIG. 2 is a schematic diagram of the overall structure of case two of the present invention;
FIG. 3 is a diagram illustrating an initial state of a chip insertion according to the present invention;
FIG. 4 is a diagram of the case two chip insertion initial state of the present invention;
FIG. 5 is a diagram of an operation state of the case of the present invention;
FIG. 6 is a diagram of the operation state of case two of the present invention;
FIG. 7 is a diagram of the structure of the PCR reaction chip of the present invention;
fig. 8 is a structural view of the guide slot of the present invention;
fig. 9 is a structural view of the chip mounting module of the present invention;
FIG. 10 is a structural view of the temperature control device of the present invention;
FIG. 11 is a structural diagram of the temperature control floating module of the present invention;
fig. 12 is a diagram of an optical module structure according to an embodiment of the present invention;
FIG. 13 is a block diagram of an optical module according to example two of the present invention;
fig. 14 is a schematic view of an optical path structure according to an embodiment of the present invention;
fig. 15 is a schematic diagram of the structure of the second optical path in the present invention.
Detailed Description
The technical solution of the present invention will be further explained with reference to the accompanying drawings and examples.
The portable ultra-fast real-time fluorescence quantitative PCR instrument shown in FIGS. 1-6 comprises: PCR reaction chip 1, chip installation module 2, temperature control floating module 3, optical module 4.
As shown in fig. 7, the PCR reaction chip 1 includes a chip substrate 101, a reaction groove 102 is formed on the chip substrate 101, a bottom film 103 is attached to a surface of the reaction groove 102, and a reaction channel is formed between the reaction groove 102 and the reaction groove 102; the chip substrate 101 is provided with a cylindrical sample adding hole 104, an opening at the bottom of the cylindrical sample adding hole 104 is communicated with one end of the reaction channel to form a sample adding channel L1, an opening at the upper end of the hole wall of the cylindrical sample adding hole 104 extends through the hole wall and is communicated with the other end of the reaction channel to form an air outlet channel L2, the cylindrical sample adding hole 104 is connected through a sample adding hole cover 105 in a threaded manner to realize sealing, an extrusion head 106 is arranged at the position of the sample adding hole cover 105 corresponding to the cylindrical sample adding hole 104, and the extrusion head 106 extrudes a sample into the reaction channel along with the rotation of the sample adding hole cover 105. The reaction tank 12 is of a flat waist-shaped channel structure, the cylindrical sampling hole 104 is of an external thread cylinder structure, the corresponding sampling hole cover 105 is of an internal thread cover structure, the cylindrical sampling hole 104 is of a three-section structure, the upper part of the cylindrical sampling hole is of an open circular truncated cone structure, the middle part of the cylindrical sampling hole is of a cylindrical structure, the bottom of the cylindrical sampling hole is of a conical structure, and the center of the bottom of the conical hole is provided with a hole; the extrusion head 106 is a cylindrical structure which is in interference fit with the diameter of the middle part of the cylindrical sample adding hole 104, the extrusion head 106 is a soft sealing column and can extrude all liquid in the sample adding hole 104 into the reaction channel, and a sealing ring 108 is arranged at the position of the inner bottom of the sample adding hole cover 105, which corresponds to the orifice of the cylindrical sample adding hole 104.
The chip belongs to an integral consumable, and different freeze-drying reagents are pre-installed in chips of different detection items.
As shown in fig. 8, the chip mounting module 2 includes a chip mounting base plate 201, a guide slot 202 is disposed on the mounting base plate 201, and the PCR reaction chip 1 is disposed in the guide slot 202; positioning grooves 107 are formed in two sides of the PCR reaction chip 1, positioning bumps are arranged on the guide slots 202 corresponding to the positioning grooves 107, and a first in-place sensor 203 is arranged.
As shown in fig. 9, the chip mounting module 2 further includes a chip sliding bottom plate 204, the chip sliding bottom plate 204 is provided with a chip guide rail 206 and a second in-place sensor 207, the driving end is connected to the chip mounting bottom plate 201, the chip is controlled to move along the guide rail, and the position is identified by the second in-place sensor 207.
As shown in fig. 10, the temperature control floating module 3 includes a temperature control device 301, a heating and cooling plate 302 and a temperature sensor 303 are disposed at an end of the temperature control device 301, a heating and cooling carrier plate 304 corresponding to the PCR reaction chip 1 is disposed on the heating and cooling plate 302, heat-insulating press plates 305 are disposed on two sides of the heating and cooling carrier plate 304, and the heat-insulating press plates 305 press the temperature control device 301.
As shown in fig. 11, the temperature control device 301 is disposed on a temperature control main bracket 307 through an L-shaped fixing bracket 306, the temperature control device 301 is connected with the L-shaped fixing bracket 306 through a first temperature control guide rail 308, and meanwhile, the end portion is connected through a pre-tightening spring 309 to achieve extrusion buffering, the L-shaped fixing bracket 306 is disposed on the temperature control main bracket 307 through a second temperature control guide rail 310, the temperature control device 301 is driven by a second motor 311 to move towards the PCR reaction chip 1, and a third in-place sensor 312 is disposed.
As shown in fig. 12 to 15, the optical module 4 includes a switching device 401 and a position sensor 402; the back surface of the chip mounting base plate 201 is provided with a through hole 208 as a part of a light path for passing excitation light and fluorescence. The light path of the optical module 4 generates excitation light by the excitation light source 403, passes through the optical filter 404 and the lens 405, and is reflected to the chip reaction tank 102 by the secondary beam splitter 406, and the generated fluorescence is received by the fluorescence detection device through the secondary beam splitter 406, wherein the fluorescence detection device is a photodiode 407, a camera 408 or a PMT.
Before testing, a sample and a reagent are added into a sample inlet by a quantitative suction pipe, then a chip screw cap is screwed down, the mixed liquid is completely extruded into a reaction channel and filled by extrusion of an encapsulation part at the end part of the screw cap, then the chip is inserted into a chip guide module along a guide device, the chip is positioned by a positioning groove, and a sensor detects whether the chip is inserted in place.
After the chip is inserted in place, the chip moves up and down under the driving of the motor, so that the center of the chip is superposed with the center of the heating and cooling carrier plate, and preparation is made for subsequent compression.
The motor drives the temperature control module to move back and forth in a plane, so that the heating and refrigerating carrier plate is close to and away from the chip, the spring enables the temperature control module to have certain pressing force, the heating and refrigerating carrier plate is tightly pressed on the surface of the chip through overpressure, a reaction cavity on the chip is tightly attached to the heating and refrigerating carrier plate, meanwhile, the periphery of the chip is surrounded by the heat preservation pressing plate to form a heat preservation layer, and reagents in the reaction cavity of the chip are uniformly heated and are quickly heated and cooled.
The optical module integrates and couples optical components such as a lens, an optical filter, a secondary spectroscope and the like, so that the optical path is simplified, the detection stability and the detection speed are improved, and the output wavelengths of the light source are 470nm, 520 nm, 570nm and 635nm respectively.
The light source is electrified and coupled to generate monochromatic light, the monochromatic light is emitted to the reaction cavity on the chip through the secondary spectroscope, and the generated fluorescence is detected by the fluorescence detection device through the secondary spectroscope and the optical filter.
Claims (6)
1. The utility model provides a portable ultrafast real-time fluorescence quantitative PCR appearance which characterized in that includes:
the PCR reaction chip (1) comprises a chip substrate (101), wherein a reaction groove (102) is formed in the chip substrate (101), a bottom film (103) is attached to the surface where the reaction groove (102) is located, and a reaction channel is formed by the bottom film and the reaction groove (102);
the chip mounting module (2) comprises a chip mounting base plate (201), a guide slot (202) is formed in the mounting base plate (201), and the PCR reaction chip (1) is arranged in the guide slot (202);
the temperature control floating module (3) comprises a temperature control device (301), a heating and refrigerating plate (302) and a temperature sensor (303) are arranged at the end part of the temperature control device (301), a heating and refrigerating carrier plate (304) corresponding to the PCR reaction chip (1) is arranged on the heating and refrigerating plate (302), heat-insulating press plates (305) are arranged on two sides of the heating and refrigerating carrier plate (304), and the heating and refrigerating carrier plate is pressed on the temperature control device (301) through the heat-insulating press plates (305);
an optical module (4) comprising a switching device (401) and a reach sensor (402); the back surface of the chip mounting base plate (201) is provided with a through hole (208) which is used as a part of a light path and is used for exciting light and fluorescence to pass through.
2. The portable ultrafast real-time fluorescence quantitative PCR instrument of claim 1, wherein: be equipped with tube-shape application of sample hole (104) on chip base member (101), tube-shape application of sample hole (104) bottom trompil forms application of sample passageway (L1) with reaction channel's one end intercommunication, tube-shape application of sample hole (104) pore wall upper end trompil extends through the pore wall and forms air outlet channel (L2) with reaction channel's other end intercommunication, tube-shape application of sample hole (104) seals through application of sample handhole door (105), application of sample handhole door (105) correspond tube-shape application of sample hole (104) position and are equipped with extrusion head (106), along with application of sample handhole door (105) rotatory seal, extrusion head (106) are crowded the sample in the reaction channel.
3. The portable ultrafast real-time fluorescence quantitative PCR instrument of claim 1, wherein: positioning grooves (107) are formed in two sides of the PCR reaction chip (1), positioning bumps are arranged on the guide slots (202) corresponding to the positioning grooves (107), and a first in-place sensor (203) is arranged.
4. The portable ultrafast real-time fluorescence quantitative PCR instrument of claim 1, wherein: the chip mounting module (2) further comprises a chip sliding base plate (204), a chip guide rail (206) and a second in-place sensor (207) are arranged on the chip sliding base plate (204), the driving end is connected with the chip mounting base plate (201), the chip is controlled to move along the guide rail, and the position is identified through the second in-place sensor (207).
5. The portable ultrafast real-time fluorescent quantitative PCR instrument according to claim 1, wherein: temperature control device (301) are arranged in on control by temperature change main support (307) through L type mount (306), temperature control device (301) are connected with L type mount (306) through first control by temperature change guide rail (308), and the tip passes through pretension spring (309) to be connected simultaneously, realize the extrusion buffering, on control by temperature change main support (307) is arranged in through second control by temperature change guide rail (310) in L type mount (306), drive temperature control device (301) through second motor (311) and move to PCR reaction chip (1) to set up the third sensor (312) that targets in place.
6. The portable ultrafast real-time fluorescence quantitative PCR instrument of claim 1, wherein: the light path of the optical module (4) generates exciting light by an exciting light source (403), the exciting light passes through an optical filter (404) and a lens (405), the exciting light is reflected to the chip reaction tank (102) by a secondary spectroscope (406), and the generated fluorescent light is received by a fluorescent light detection device through the secondary spectroscope (406), wherein the fluorescent light detection device is a photodiode (407) or a camera (408) or a PMT.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220646804.9U CN217351365U (en) | 2022-03-23 | 2022-03-23 | Portable ultra-fast real-time fluorescence quantitative PCR instrument |
| PCT/CN2022/083373 WO2023178710A1 (en) | 2022-03-23 | 2022-03-28 | Portable ultrafast real-time fluorescent quantitative pcr instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220646804.9U CN217351365U (en) | 2022-03-23 | 2022-03-23 | Portable ultra-fast real-time fluorescence quantitative PCR instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217351365U true CN217351365U (en) | 2022-09-02 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202220646804.9U Active CN217351365U (en) | 2022-03-23 | 2022-03-23 | Portable ultra-fast real-time fluorescence quantitative PCR instrument |
Country Status (2)
| Country | Link |
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| CN (1) | CN217351365U (en) |
| WO (1) | WO2023178710A1 (en) |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100695148B1 (en) * | 2005-05-02 | 2007-03-14 | 삼성전자주식회사 | Module for polymerase chain reaction and multiple polymerase chain reaction system |
| CN106929408B (en) * | 2017-01-26 | 2018-07-20 | 深圳市尚维高科有限公司 | Portable micro-fluidic PCR instrument and gene samples fluorescence quantitative detecting method |
| CN106957788A (en) * | 2017-03-19 | 2017-07-18 | 北京化工大学 | A kind of multichannel real-time fluorescence quantitative PCR micro-fluidic chip system |
| CN107653186A (en) * | 2017-09-15 | 2018-02-02 | 广东达元食品药品安全技术有限公司 | A kind of portable PCR instrument |
| KR102261902B1 (en) * | 2018-09-28 | 2021-06-08 | 주식회사 바이오메듀스 | Real-time PCR Fluorescence detection device |
| CN111607509B (en) * | 2019-02-22 | 2023-04-07 | 西安天隆科技有限公司 | Automatic amplification and detection device for digital PCR |
| CN110551622A (en) * | 2019-09-18 | 2019-12-10 | 无锡百泰克生物技术有限公司 | quick PCR reaction chip and quick fluorescence quantitative detector |
| CN210945600U (en) * | 2019-09-18 | 2020-07-07 | 无锡百泰克生物技术有限公司 | Quick PCR reaction chip and quick fluorescence quantitative detector |
| CN213506974U (en) * | 2020-07-31 | 2021-06-22 | 北京贝泰科技有限公司 | Portable real-time fluorescent quantitative PCR instrument |
| CN214300158U (en) * | 2020-12-01 | 2021-09-28 | 无锡百泰克生物技术有限公司 | Quick fluorescence quantitative determination appearance |
| CN113832022A (en) * | 2021-09-23 | 2021-12-24 | 吉特吉生物技术(苏州)有限公司 | Integrated nucleic acid amplification detection equipment |
-
2022
- 2022-03-23 CN CN202220646804.9U patent/CN217351365U/en active Active
- 2022-03-28 WO PCT/CN2022/083373 patent/WO2023178710A1/en unknown
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| WO2023178710A1 (en) | 2023-09-28 |
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