CN215856121U - Portable real-time fluorescence quantitative PCR instrument - Google Patents

Abstract

The utility model provides a portable real-time fluorescence quantitative PCR instrument, which comprises a box body, a heating and cooling unit, a light source transmitting and receiving unit, a display unit and a power supply. The box is provided with and carries dish and slide rail, carries to be provided with a plurality of standing grooves of arranging into a column on the dish. Place the test bottle in the standing groove, open heating cooling unit and carry out the circulation heating to the test bottle, can make the DNA fragment in the test bottle amplify rapidly. And simultaneously, the light source transmitting and receiving unit is aligned to the test bottle through the slide rail to mark and monitor the product, and the real-time fluorescence quantity is obtained. The excited fluorescent substance is converted into an electric signal by collecting the fluorescent signal, and then the solution concentrations with different concentrations are obtained by calculating the electric signals with different intensities through corresponding formulas, so as to obtain the detected data and finally obtain the required detected data. And the obtained detection data can be sent to the display unit for displaying, so that an operator can visually look up the detected data.

Description

Portable real-time fluorescence quantitative PCR instrument

Technical Field

The utility model relates to the field of detection instruments, in particular to a portable real-time fluorescence quantitative PCR instrument (English is called polymerase chain reaction, Chinese translation is polymerase chain reaction).

Background

The epidemic is always the subject of human research, such as african swine fever, and the laboratory detection technology of african swine fever: the nucleic acid detection technology based on polymerase chain reaction (PCR for short, polymerase chain reaction) has the advantages of easy operation, high sensitivity, strong specificity, low requirement on samples and the like, can realize early diagnosis, and is one of important means for detecting epidemic diseases in an epidemic area.

The Polymerase Chain Reaction (PCR) is a nucleic acid amplification technique that simulates the natural DNA replication process in vitro, and the principle is similar to the natural DNA replication, and is a method for selectively synthesizing specific DNA by in vitro enzymatic reaction. The real-time fluorescent quantitative PCR reaction is carried out in a cycle consisting of high-temperature thermal denaturation, low-temperature renaturation and suitable-temperature extension by adding fluorescent dye, so that the DNA fragments can be rapidly amplified. And in the whole circulation process, the product is marked and monitored, and a fluorescence detection device is utilized to obtain the real-time fluorescence quantity.

For fluorescence detection, however, spectra are required. For the wide application of optical spectrum in various fields, fluorescence detection for detecting cells, bacteria, mold and the like is an important detection means, and the principle of the fluorescence detection optical system comprises that an excitation light source is projected on a biochip, a fluorescent substance is excited, a fluorescence signal is collected and projected on a photosensitive device, the optical signal is converted into an electric signal, the concentration of solutions with different concentrations is obtained through calculation of corresponding formulas through electric signals with different intensities, and then detected data is obtained.

Traditional fluorescence detection optical system, only one excitation light system can detect a reagent sample of a passageway the inside, when a plurality of passageways detection samples of needs, then can lead to optical system bulky, be difficult for integrated inside the instrument, also there is through optic fibre or leaded light strip material at present, guide light source signal to same emission light source module inside and guide to the photosensor module of receiving fluorescence signal, but often behind the passageway optic fibre guide, transmission and received light intensity signal all weaken by a wide margin, need improve the light intensity through the power that increases the lamp source and increase optical lens group.

In the prior art, the light path is transmitted and received by a mode of realizing light path steering through a dichroic mirror based on an optical conjugate system, the structure of the light path system is complex, the angle of an optical device is difficult to adjust, and the fine adjustment influence is great (the angle of the optical device needs to be fine adjusted, so the adjustment precision and difficulty are great). The whole structure has large volume, can only be used for a single light source, and cannot be applied to real-time fluorescence quantitative analysis under a variable temperature control system.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a portable real-time fluorescence quantitative PCR instrument, which aims to solve the problems that in the prior art, optical fiber is adopted to conduct and detect light sources, the detection precision is poor, and the traditional detection instrument is large in size and not easy to carry.

In order to realize the purpose of the utility model, the technical scheme adopted by the utility model is as follows: a portable real-time fluorescent quantitative PCR instrument comprising: the box body is provided with a carrying disc on the inner bottom surface, the carrying disc is provided with a plurality of placing grooves which are arranged in a line, and the inner bottom surface of the box body is provided with a sliding rail which is parallel to the placing grooves in a line; the heating and cooling unit is arranged between the box body bottom plate and the carrying disc and is used for circularly adjusting the temperature of the carrying disc; the light source transmitting and receiving unit is arranged on the slide rail, and a light emitting port and a light inlet of the light source transmitting and receiving unit are both arranged towards the carrying disc; the display unit is arranged on the top surface of the box body and is in wireless communication or wired connection with the light source transmitting and receiving unit; and the power supply is arranged in the box body and is used for providing electric energy for the portable real-time fluorescence quantitative PCR instrument.

Preferably, the heating and cooling unit is a split semiconductor heating and cooling sheet, the heating and cooling unit is provided with an exhaust fan between the bottom surface of the box body, and the exhaust fan is located on the bottom plate and/or the side plate of the box body.

Preferably, the placing grooves are provided with two rows, and the light source transmitting and receiving units are provided with two groups and are symmetrically arranged on two sides of the carrying disc.

Preferably, the light source transmitting and receiving unit comprises a light source receiver, a light source emitter and a frame body, the light source emitter and the frame body are at least located, the light source emitter is arranged on one side of the light source receiver, the frame body is used for accommodating the light source receiver and the light source emitter, the light source receiver and the light source generator are provided with light path included angles of incident light and reflected light, and the frame body is arranged on the sliding rail.

Preferably, the light source receiver is provided with an optical filter, a plano-convex lens and a photosensitive sensor in sequence along a receiving light path.

Preferably, the light source emitter has set gradually lamp plate, holding along the transmission light path the diaphragm seat and the battery of lens of lamp plate, the lamp plate is provided with the luminous light source of at least one kind.

Preferably, a heat insulation plate is arranged between the carrying disc and the heating and cooling unit, and a window is formed in the joint of the heat insulation plate between the carrying disc and the heating and cooling unit.

Preferably, a detachable or turnover heat-insulating heating block is further arranged in the box body, and the heat-insulating heating block is located right above the carrying disc.

Preferably, the carrying disc is made of aluminum alloy, and a hollow groove is formed between the placing groove of the carrying disc and the placing groove.

Preferably, a printer is further arranged in the box body, and the printer is electrically connected with the light source transmitting and receiving unit.

Compared with the prior art, the utility model has the following beneficial effects:

1. place the test bottle in the standing groove on year dish earlier, and then open heating cooling unit and carry out the circulation heating to the test bottle, cool down after heating to the uniform temperature promptly, descend to reheating behind the uniform temperature, so relapse, can make the DNA fragment in the test bottle can amplify rapidly. Meanwhile, the light source transmitting and receiving unit is aligned to the test bottle through sliding adjustment of the sliding rail, the light source transmitting and receiving unit is started to transmit corresponding detection light, and in the whole circulation process, products are marked and monitored, and real-time fluorescence quantity is obtained. The excited fluorescent substance is converted into an electric signal by collecting the fluorescent signal, and then the solution concentrations with different concentrations are obtained by calculating the electric signals with different intensities through corresponding formulas, so as to obtain the detected data and finally obtain the required detected data. And the obtained detection data can be sent to the display unit for displaying, so that an operator can visually look up the detected data.

2. The separated semiconductor heating and refrigerating sheet can be used for switching heating and refrigerating, and heating, heating and refrigerating operations are performed on the carrying disc arranged on the separated semiconductor heating and refrigerating sheet. Meanwhile, the use of the exhaust fan is combined, the ambient temperature in the box body can be coordinated and controlled, the influence of the ambient temperature on the test bottle is avoided, and the detection accuracy is improved.

3. When the test tube is heated circularly, condensed water drops are easily formed on the upper part of the tube opening, and the detection of illumination is further influenced. Therefore, the top of the test tube can be heated by the heat preservation heating block right above, condensation of water drops is avoided, the influence of the water drops and the temperature difference is eliminated, and the detection precision is improved.

Drawings

FIG. 1 is a perspective view of a portable real-time fluorescence quantitative PCR instrument according to an embodiment;

FIG. 2 is a side view of a portable real-time fluorescence quantitative PCR instrument according to an embodiment;

FIG. 3 is a partial view of a portable real-time fluorescence quantitative PCR instrument according to an embodiment;

FIG. 4 is an enlarged partial view of the ellipse indicated by the mark A in FIG. 3;

FIG. 5 is a cross-sectional view of a portable real-time fluorescence quantitative PCR instrument according to an embodiment;

FIG. 6 is an enlarged partial view of the circle indicated by B in FIG. 5;

FIG. 7 is a partial cross-sectional view of the portable real-time fluorescence quantitative PCR instrument according to the embodiment.

Reference numerals:

10. a box body; 101. a carrying tray; 102. a slide rail; 103. a heat insulation plate;

104. a heat preservation heating block; 105. a printer;

1011. a placement groove; 1012. hollowing out the grooves;

1021. a stepping motor; 1022. rotating the screw rod; 1023. a track; 1024. a slider;

20. a heating and cooling unit; 201. an exhaust fan; 202. air exhaust mesh holes;

30. a light source transmitting and receiving unit;

301. a light source receiver; 302. a light source emitter; 303. a frame body;

3011. an optical filter; 3012. a plano-convex lens; 3013. a photosensitive sensor;

3021. a lamp panel; 3022. a diaphragm seat; 3023. a lens group;

40. a display unit;

50. a power source.

Detailed Description

The utility model proposes a new solution, which is described in detail below with reference to the accompanying drawings for a clearer representation.

Referring to fig. 1 to 7, the present embodiment provides a portable real-time fluorescence quantitative PCR instrument, which includes a box 10, a heating and cooling unit 20, a light source emitting and receiving unit 30, a display unit 40 and a power supply 50, wherein a carrying tray 101 is disposed on an inner bottom surface of the box 10, a plurality of placing slots 1011 are disposed on the carrying tray 101, and the placing slots 1011 in a row are designed to be at equal intervals. The inner bottom surface of the box 10 is provided with slide rails 102 parallel to the row of the placement grooves 1011. The heating and cooling unit 20 is arranged between the bottom plate of the box body 10 and the carrying disc 101, and the heating and cooling unit 20 is used for circularly adjusting the temperature of the carrying disc 101. The light source transmitting and receiving unit 30 is arranged on the slide rail 102, the light emitting port and the light inlet of the light source transmitting and receiving unit 30 are arranged towards the carrying disc 101, and the light source transmitting and receiving unit 30 can be close to the test bottle on the placing groove 1011, so that the utilization rate of light energy can be improved, and the light source transmitting and receiving unit can emit and receive light. The display unit 40 is disposed on the top surface of the box 10, and is in wireless communication or wired connection with the light source transmitting and receiving unit 30, and the display unit 40 may be a touch display screen. The power supply 50 is disposed in the casing 10 and is used for supplying electric power to the portable real-time fluorescence quantitative PCR instrument.

As shown in fig. 1 and fig. 2, the portable real-time fluorescence quantitative PCR instrument in this embodiment has the advantages of being convenient to carry, simple to operate, and relatively high in detection accuracy. Specifically, the box 10 is a box 10, and the box 10 may be a split box 10 or a box 10 with a flip-top cover. The handle and/or the pull handle and/or the universal wheels on the box body 10 can assist the user to take and carry the box body 10, and the carrying convenience is improved. The box body 10 can also be made of metal materials, such as aluminum alloy, titanium alloy and the like, and has the characteristics of high strength and light weight, so that internal devices are protected, and the problem that the box body 10 is heavy is avoided.

As shown in fig. 5 and 6, in use, a test bottle is placed in the placement groove 1011 of the boat 101. Because the standing groove 1011 has a plurality ofly, can place different test bottles in the standing groove 1011 of difference to it can to place corresponding quantity test bottle according to actual conditions. After the test bottle is placed, the heating and cooling unit 20 is started to circularly heat the test bottle, namely, the test bottle is cooled after being heated to a certain temperature, and then is heated again after being cooled to a certain temperature, and the process is repeated. For example, the cyclic heating temperature is: the temperature is cyclically heated and reduced between 60 ℃ and 90 ℃, and a period is formed by high-temperature thermal denaturation, low-temperature renaturation and proper-temperature extension and is cyclically carried out, so that the DNA fragments in the test bottle can be rapidly amplified.

While the DNA fragments are amplified, the light source transmitting and receiving unit 30 is aligned to the test bottle through the sliding adjustment of the slide rail 102, and the light source transmitting and receiving unit 30 is started to emit corresponding detection light, such as fluorescence, and the products are marked and monitored in the whole circulation process, and the real-time fluorescence quantity is obtained. The excited fluorescent substance is converted into an electric signal by collecting the fluorescent signal, and then the solution concentrations with different concentrations are obtained by calculating the electric signals with different intensities through corresponding formulas, so as to obtain the detected data and finally obtain the required detected data.

The obtained detection data is sent to the display unit 40 for display, so that the operator can visually refer to the detected data. The inside of the box body 10 can be in communication connection with an external terminal through a wireless communication module, the external terminal can be a mobile phone, a notebook computer and the like, and further corresponding detection data can be obtained on the external terminal in time.

As shown in fig. 3 and 4, in this embodiment, the slide rail 102 includes a stepping motor 1021, a rotary screw rod 1022, a track 1023 and a slider 1024, the stepping motor 1021 is electrically connected with the power source 50, the rotary screw rod 1022 is disposed on an output shaft of the stepping motor 1021, the slider 1024 is slidably disposed on the track 1023, a threaded hole matched with the rotary screw rod 1022 is disposed on the slider 1024, when the stepping motor 1021 drives the rotary screw rod 1022 to rotate, the slider 1024 is driven to move back and forth on the track 1023 through the matching of threads, and precise adjustment can be achieved. And the light source transmitting and receiving unit 30 is installed on the sliding block 1024 and slides along with the sliding block 1024, so that the light source transmitting and receiving unit 30 is moved to one side of different placing grooves 1011, and the test bottles in the placing grooves 1011 are detected.

As shown in FIG. 5, the power source 50 may be a lithium battery, which can be repeatedly charged for use, so that the portable real-time fluorescence quantitative PCR instrument can be carried to the field for use. Of course, the portable real-time fluorescence quantitative PCR instrument can also be directly connected with commercial power for use so as to ensure the working time of the portable real-time fluorescence quantitative PCR instrument.

As shown in fig. 7, in the present embodiment, the heating and cooling unit 20 is a separate semiconductor heating and cooling sheet, an exhaust fan 201 is disposed between the heating and cooling unit 20 and the bottom surface of the box 10, and an exhaust mesh 202 is disposed on a bottom plate and/or a side plate of the box 10 at the position of the exhaust fan 201. The separated semiconductor heating and cooling piece can switch heating and cooling, and perform heating and cooling operations on the loading disc 101 mounted on the separated semiconductor heating and cooling piece. The environment temperature inside the box body 10 also needs to be controlled, heat dissipation can be carried out through the exhaust fan 201, the temperature of the internal environment can be coordinately controlled, the influence of the environment temperature on the test bottle is avoided, and the accuracy of detection is improved.

In order to enable the carrying disc 101 to conduct heat well, the carrying disc 101 is made of aluminum alloy, the carrying disc 101 is light in weight, and meanwhile has good heat conductivity, and a hollow groove 1012 is formed between the placing groove 1011 and the placing groove 1011 of the carrying disc 101. The design of the hollow-out grooves 1012 is adopted at the redundant positions of the carrying disc 101, so that the weight of the carrying disc 101 can be further reduced, meanwhile, the heating and refrigerating efficiency of the temperature can be higher, and the detection efficiency is improved.

In this embodiment, a heat insulation plate 103 is disposed between the carrying tray 101 and the heating and cooling unit 20, and a window is formed at a joint of the heat insulation plate 103 between the carrying tray 101 and the heating and cooling unit 20. The heating and cooling unit 20 further has a temperature sensor capable of feeding back the temperature of the tray 101 in real time, and the separated semiconductor heating/cooling fins perform precise temperature cycling operation by using the information fed back by the temperature sensor. The volume of the separated semiconductor heating and refrigerating piece is larger than that of the carrying disc 101, so that temperature radiation is easily caused to the test bottle, and the detection precision is influenced. The carrying disc 101 can be connected and installed through a window on the heat insulation plate 103 by separating through the heat insulation plate 103, and the use convenience is guaranteed.

As shown in fig. 1, in this embodiment, a detachable or foldable thermal insulation heating block 104 is further disposed in the box body 10, and the thermal insulation heating block 104 is located right above the tray 101. The heat preservation and heating block 104 may also be a semiconductor heating and cooling sheet, or an electric heating plate. When the test tube is heated circularly, condensed water drops are easily formed on the upper part of the tube opening, and the detection of illumination is further influenced. Therefore, the top of the test tube can be heated by the heat preservation and heating block 104 right above, condensation of water drops is avoided, the influence of the water drops and the temperature difference is eliminated, and the detection precision is improved. The laminate on which the heat-insulating and heating block 104 is located may be embedded and spliced with the box body 10, for example, the cross section of the inner cavity of the box body 10 is rectangular, the laminate is also rectangular, and the laminate is clamped in the box body 10 by using a shoulder extending outwards from the edge of the laminate, so that the heat-insulating and heating block 104 can heat the test bottle. Or the heat-insulating heating block 104 is arranged on the flip cover by utilizing the flip design, and the flip cover is arranged right above the carrying disc 101 to heat and insulate the test bottle, so that the test bottle can be conveniently replaced through the flip cover.

The two rows of the placing slots 1011 are provided in the embodiment, and the two groups of the light source transmitting and receiving units 30 are symmetrically arranged on two sides of the carrying tray 101, and the two light source transmitting and receiving units 30 are driven by the two sliding rails 102 respectively. And through increasing the standing groove 1011, can improve the quantity of placing the test bottle, and then reduce the time of changing the test bottle, improve detection efficiency.

As shown in fig. 5 and fig. 6, in the present embodiment, the light source transmitting and receiving unit 30 includes a light source receiver 301, a light source emitter 302 at least disposed on one side of the light source receiver 301, and a frame 303 for accommodating the light source receiver 301 and the light source emitter 302, an included angle between light paths of incident light and reflected light is set between the light source receiver 301 and the light source emitter, and the frame 303 is disposed on the sliding rail 102. The light source receiver 301 is provided with a filter 3011, a plano-convex lens 3012, and a photosensor 3013 in this order along a reception optical path. The light source emitter 302 is provided with a lamp panel 3021, a diaphragm seat 3022, and a lens group 3023 in this order along an emission light path.

Specifically, the light source transmitting and receiving unit 30 has a frame 303, and the light source receiver 301 and the light source transmitter 302 are mounted in a limited manner through the frame 303. The included angle of the light path between the light source emitter 302 and the light source receiver 301 is ensured, so that the light path emitted by the light source emitter 302 is reflected by the test bottle and then received by the light source receiver 301. The stability of the detection light path is ensured, the deviation condition can not occur in the carrying process, and the stability of the structure is improved.

Further, a plurality of LED lamps capable of emitting different light sources are mounted on the lamp panel 3021, for example, two light source transmitters 302 are symmetrically disposed on two sides of the light source receiver 301, and two light sources can be respectively disposed on the lamp panels 3021 of the two light source transmitters 302, so that the light source transmitting and receiving unit 30 can perform detection of four different light sources. A light path channel facing the carrying disc 101 is formed in the frame 303, a lens group 3023 is placed in the light path channel, the lens group 3023 includes a plano-convex lens and a convex lens, and a lamp panel 3021 is arranged at one end of the light path channel far away from the carrying disc 101. When the lamp panel 3021 emits a light source, the light source sequentially passes through the plano-convex lens and the convex lens and enters the test bottle; after being reflected by the test bottle, the light filter 3011, the plano-convex lens 3012 and the photosensitive sensor 3013 in the light source receiver 301 sequentially receive the light, and the photosensitive sensor 3013 feeds back the acquired information to the display unit 40 and/or the external terminal, thereby completing the detection of the test bottle.

By using the optical path detection of the light source transmitting and receiving unit 30 of the present embodiment, the conventional structure of using optical fiber to conduct the optical path is eliminated, so that the loss of light energy in the optical fiber is avoided, and the detection precision is improved.

When the reagent bottle detection device is used, the position of the sliding rail 102 can be adjusted by controlling keys on the touch display screen, so that the light source transmitting and receiving unit 30 is aligned to the reagent bottle to be detected, one or more light sources can be used for detection, and the convenience of operation and control is improved. In order to facilitate the operation, an intelligent language module can be arranged, and a microphone is used for inputting language to control the portable real-time fluorescence quantitative PCR instrument to detect.

As shown in fig. 1, in the present embodiment, a printer 105 is further disposed in the box 10, and the printer 105 is electrically connected to the light source transmitting and receiving unit 30. The detection result can be printed by the printer 105, which facilitates printing of a paper report.

Although the present invention has been described in detail in the foregoing embodiments, it should be understood by those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof without departing from the spirit and scope of the utility model.

Claims (10)

1. A portable real-time fluorescence quantitative PCR instrument is characterized by comprising:

the box body is provided with a carrying disc on the inner bottom surface, the carrying disc is provided with a plurality of placing grooves which are arranged in a line, and the inner bottom surface of the box body is provided with a sliding rail which is parallel to the placing grooves in a line;

the heating and cooling unit is arranged between the box body bottom plate and the carrying disc and is used for circularly adjusting the temperature of the carrying disc;

the light source transmitting and receiving unit is arranged on the slide rail, and a light emitting port and a light inlet of the light source transmitting and receiving unit are both arranged towards the carrying disc;

the display unit is arranged on the top surface of the box body and is in wireless communication or wired connection with the light source transmitting and receiving unit; and

and the power supply is arranged in the box body and is used for providing electric energy for the portable real-time fluorescence quantitative PCR instrument.

2. The portable real-time fluorescent quantitative PCR instrument according to claim 1, characterized in that: the heating and cooling unit is a separated semiconductor heating and cooling sheet, the heating and cooling unit is provided with an exhaust fan between the bottom surfaces of the boxes, and the exhaust fan is positioned at the bottom plates and/or the side plates of the boxes and is provided with air exhaust meshes.

3. The portable real-time fluorescent quantitative PCR instrument according to claim 1, characterized in that: the placing grooves are provided with two rows, and the light source transmitting and receiving units are provided with two groups and are symmetrically arranged on two sides of the carrying disc.

4. The portable real-time fluorescent quantitative PCR instrument according to claim 1, characterized in that: the light source transmitting and receiving unit comprises a light source receiver, a light source transmitter and a support body, wherein the light source transmitter and the support body are arranged on one side of the light source receiver, the light source receiver and the support body are accommodated, the light source receiver and the light source generator are provided with light path included angles of incident light and reflected light, and the support body is arranged on the sliding rail.

5. The portable real-time fluorescent quantitative PCR instrument according to claim 4, wherein: the light source receiver is sequentially provided with an optical filter, a plano-convex lens and a photosensitive sensor along a receiving light path.

6. The portable real-time fluorescent quantitative PCR instrument according to claim 4, wherein: the light source emitter has set gradually lamp plate, holding along the transmission light path the diaphragm seat and the battery of lens of lamp plate, the lamp plate is provided with the luminous light source of at least one kind.

7. The portable real-time fluorescent quantitative PCR instrument according to claim 1, characterized in that: and a heat insulation plate is arranged between the carrying disc and the heating and cooling unit, and a window is formed in the joint of the heat insulation plate between the carrying disc and the heating and cooling unit.

8. The portable real-time fluorescent quantitative PCR instrument according to claim 1, characterized in that: still be provided with in the box and can dismantle or flip's heat preservation heating block, heat preservation heating block is located directly over the year dish.

9. The portable real-time fluorescent quantitative PCR instrument according to claim 1, characterized in that: the carrying disc is made of aluminum alloy, and a hollow groove is formed between the placing groove of the carrying disc and the placing groove.

10. The portable real-time fluorescent quantitative PCR instrument according to claim 1, characterized in that: and a printer is also arranged in the box body and is electrically connected with the light source transmitting and receiving unit.

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