JP2006322720A - Absorptiometer with microchip - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an absorptiometer with a microchip which sets a measuring liquid comprising a liquid to be inspected and a reagent supplied into the microchip into the state including an absorption component when the microchip is inserted into the device. <P>SOLUTION: This absorptiometer is equipped with the microchip having an absorptiometric part, a chip holder having a chip integration space wherein the microchip is integrated, an optical member for introducing the light radiated from a light source into the absorptiometric part in the microchip, and a photodetector for receiving the light radiated from the light source and transmitted through the absorptiometric part in the microchip. The device has characteristics wherein the microchip is held horizontally by the chip holder, and the chip holder is provided with a temperature control mechanism for controlling the microchip temperature. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a microchip absorptiometry apparatus for performing solution analysis by spectrophotometry using a microchip.

In recent years, micromachine fabrication technology has been applied to use micro-TAS (μ-Total Analysis System) and microchips called “Lab on chip”, which perform chemical analysis and the like with a smaller size than conventional devices. Analysis methods are attracting attention.
When such an analysis system using a microchip (hereinafter also referred to as a “microchip analysis system”) is used in the medical field, a liquid to be examined such as blood (hereinafter referred to as “subject to be analyzed”) required for analysis. The amount of test solution ") can be reduced, so that the burden on the patient can be reduced and the amount of reagent can be reduced, so that the analysis cost can be reduced and the apparatus is small. Therefore, there is an advantage that analysis can be easily performed.

  According to such a microchip analysis system, blood collected by, for example, a painless needle is introduced into a microchip in which a fine flow path is formed by an absorptiometric analysis method, and is introduced into the flow path in the microchip. The blood is centrifuged to separate plasma and blood cells, the separated plasma is uniformly mixed with a reagent and a mixer to obtain a measurement target liquid, and the light emitted from the light source to the measurement target liquid Is necessary for diagnosing liver function by performing a series of analysis operations in the flow path of the microchip, such as measuring the amount of attenuation of light of a specific wavelength by irradiating -Enzyme concentration in plasma in blood such as GTP (γ-glutamyl transpeptidase) and GPT (glutamate-pyruvate transaminase) The degree can be analyzed (for example, refer to Patent Document 1).

  In Patent Document 1, as a device for analyzing the concentration of an enzyme contained in plasma by a microchip analysis system, a light incident port having an opening on the top surface of the microchip is provided at one end of the flow path of the microchip. At the same time, the portion where the other end is provided with a light exit having an opening on the top surface of the microchip is used as an absorptiometry unit, and light emitted from a light source such as a light emitting diode is absorbed from the light entrance through the top surface of the microchip. A configuration in which light incident on the photometric measurement unit and totally reflected by the spectrophotometric measurement unit and emitted from the light exit port is received by a photodetector such as a silicon photodiode provided on the upper surface of the microchip. Proposed.

  As an apparatus for performing analysis by the microchip analysis system, as shown in FIG. 9, the liquid to be inspected is placed in the flow path 81 formed in the microchip 80 by the spectrophotometric analysis method or the fluorescence analysis method. The measurement target liquid obtained by adding the reagent is poured, and the linear portion 81A of the flow path 81 is used as a measurement unit for absorption intensity or fluorescence intensity, and the light emitted from the light source 82 that has passed through the measurement unit is emitted as light. A configuration in which the concentration of the detection target component in the test liquid is calculated based on the light absorption intensity or the fluorescence intensity obtained by causing the detector 83 to receive light has been proposed (see, for example, Patent Document 2 and Patent Document 3). .)

JP 2004-109099 A JP 2003-279471 A Japanese Patent Laid-Open No. 2004-72307

  In a microchip analysis system, in order to analyze a liquid to be inspected using absorptiometry, a measurement obtained by supplying a reagent together with the liquid to be inspected into a microchip and mixing them. It is necessary to make the target liquid contain a light-absorbing component by chemical reaction, and in order to make the measurement target liquid contain a light-absorbing component by chemical reaction, the liquid to be inspected and a reagent are included. It is necessary to heat the liquid to be measured to an appropriate temperature.

  The present invention has been made based on the circumstances as described above, and its purpose is to use a test target liquid and a reagent supplied in the microchip in a state where the microchip is inserted in the apparatus. Another object of the present invention is to provide a microchip absorptiometry apparatus capable of bringing a liquid to be measured into a state containing a light-absorbing component.

The microchip absorptiometry apparatus of the present invention includes a microchip having an absorptiometry unit, a chip holder having a chip incorporation space in which the microchip is incorporated, and an absorptiometry unit in the microchip that emits light emitted from a light source. And an optical member for introducing light into the microchip, and a photodetector that receives the light emitted from the light source that has been transmitted through the absorbance measurement unit of the microchip.
The microchip is held horizontally by a chip holder,
The chip holder is provided with a temperature control mechanism for controlling the temperature of the microchip.

  The microchip absorptiometry apparatus of the present invention is formed on the upper surface of the absorptiometry part and the microchip from the liquid reservoir part to be measured, which is formed in the microchip and communicates with the absorptiometer part. It is preferable that a suction mechanism for sucking the liquid to be measured to be introduced by providing a communication state is provided.

  In the microchip absorptiometry apparatus of the present invention, the light source is composed of a light emitter that emits light in a continuous wavelength region, and the wavelength selection filter is replaced on the optical path from the light emitted from the light source to the photodetector. It is preferable to be provided.

  The microchip absorptiometry apparatus of the present invention introduces a liquid to be measured into an absorptiometry unit in a microchip held in a chip holder, and measures the absorptiometry while the temperature of the microchip is controlled by a temperature control mechanism. It is characterized by performing.

  According to the microchip absorptiometry apparatus of the present invention, the temperature of the microchip inserted into the apparatus can be controlled by the temperature control mechanism. Since the temperature can be set to a temperature suitable for a chemical reaction, in the state in which the microchip is inserted into the apparatus, the measurement target liquid that is supplied into the microchip and the reagent is used as the light absorption component. It can be set as the containing state.

Hereinafter, the present invention will be described in detail.
FIG. 1 is a schematic diagram for explaining an example of the configuration of the microchip absorptiometer of the present invention, FIG. 2 is a block diagram for explaining the microchip absorptiometer of FIG. 1, and FIG. FIG. 4 is an explanatory diagram showing a configuration of a microchip constituting the microchip absorptiometer of FIG. 1, and FIG. 4 is an explanatory diagram showing a configuration of a chip holder constituting the microchip absorptiometer of FIG. .
This microchip absorptiometry apparatus (hereinafter also simply referred to as “microchip measurement apparatus”) 10 uses blood as a test liquid (test target liquid), and tests based on the absorbance measured by absorptiometry. This is for measuring the γ-GTP activity value in the liquid.

  The microchip measuring apparatus 10 has a protruding portion 32 at the tip portion (upper portion in FIG. 1), and has a rectangular parallelepiped shape extending in the horizontal direction (left and right direction in FIG. 1) in the surface direction inside the protruding portion 32. The microchip 30 in which the absorbance measuring unit 33 made of a space is formed is inserted into a built-in space 42 in a chip holder 40 made of, for example, an aluminum box-like body by being inserted through a chip mounting opening 42A, and horizontally. And the light source 13 for supplying light to the absorptiometer 33 and the light path L from the light source 13 to the chip built-in body 11. The wavelength selection filter 14 provided above, the optical member 50 for introducing the light emitted from the light source 13 into the absorptiometry unit 33, the wavelength selection filter A light detector 15 for receiving light transmitted through the absorptiometry unit 33 provided so as to face the light source 13 via the filter 14 and the microchip built-in body 11, and the light detector 15 electrically A connected control board having a function of amplifying a light intensity signal relating to received light output from the photodetector 15 and finally calculating a γ-GTP activity value based on the light intensity signal 19.

  In the example of this figure, on the control board 19, in addition to the photodetector 15, an operation switch 21 for the operator to control the operation of the microchip measuring apparatus 10 itself, the measurement results, and the microchip measuring apparatus 10 itself. An output device 22 for outputting information relating to the state of the device, an external connection terminal 23 for performing information communication with the external control device when the microchip measuring device 10 is controlled by the external control device, and a light source A fan 24 for cooling 13 is electrically connected, and a control power source 25 for supplying power to components other than the light source 13 via a control board 19 and a lighting device 26 A light source power source 27 connected to the light source 13 is electrically connected. The light source power source 27 and the control power source 25 are configured to be connected to a commercial AC power source via the terminal block 28, the noise filter 29, the power switch 18, and the AC inlet 17. Is turned on to supply power from a commercial AC power source to bring the microchip measuring apparatus 10 into an operating state. On the other hand, turning the power switch 18 into an off state puts the microchip measuring apparatus 10 into a stopped state. .

  In addition, the microchip measuring apparatus 10 is formed in a rectangular parallelepiped shape as a whole, which includes a bottom plate, a top plate, and an outer peripheral side wall plate 12A having a front plate 12B on which an openable / closable door (not shown) is formed. All the structural members are arranged in the housing 12, and in this housing 12, the light emission center of the light source 13, the optical axis of the optical member 50, the absorbance measurement unit 33 in the microchip 30, The photodetector 15 is linearly arranged.

The microchip 30 is made of a light-transmitting plastic material such as a thermoplastic resin such as polymethyl methacrylate (PMMA), polyethylene terephthalate (PET), polycarbonate (PC), or a thermosetting resin such as an epoxy resin. A plate-like body formed by joining two substrates, and a recess for an absorptiometry part formed on one substrate (hereinafter also referred to as a “first substrate”) is stacked on the first substrate. The absorptiometry unit 33 is formed by being sealed by the other substrate (hereinafter also referred to as “second substrate”) in an overlapped state.
In the example shown in the drawing, the microchip 30 includes an absorptiometric measurement unit 33 and a space formed by a recess formed in the first substrate and a second substrate, and an inspected solution injection unit 35 communicating with the outside. The reagent reservoir 36, the reagent mixing part 37, and the suction hole part 38 having the suction mechanism connection hole 38A, and the flow path that connects these, specifically, the test liquid injection part 35 and the reagent mixing part 37 are communicated with each other. Liquid channel 39A to be inspected, reagent channel 39B that communicates the reagent reservoir 36 and reagent mixing unit 37, liquid channel 39C to be measured that communicates the reagent mixing unit 37 and absorbance measuring unit 33, and the absorbance The liquid to be inspected, which has a flow path for suction 39D that communicates the measuring part 33 and the suction hole part 38, is supplied to the liquid to be inspected liquid injection part 35, and is filled in the reagent reservoir 36. And reagents for reacting with Analyte solution obtained by being mixed in medicine mixing section 37 is of a configuration to be introduced into the part for measuring absorbance 33.
In FIG. 3, 35A and 36A are air holes, and the absorbance measuring unit 33, the test liquid injection unit 35, the reagent reservoir 36, the suction hole 38 and the flow path are inside the microchip 30. However, it is indicated by a solid line for convenience.

The chip holder 40 has a function of holding the microchip 30 horizontally, specifically, holding the surface of the microchip 30 in a horizontal state.
In the example of this figure, the chip holder 40 has a linear shape in the optical axis direction (left and right direction in FIG. 1) of the absorbance measuring unit 33 in the microchip 30 that communicates with the built-in space 42 together with the built-in space 42. Aperture section for introducing light emitted from the light source 13 incident from one end (right end in FIG. 4) 44A into the absorptiometer 33 by emitting from the other end (left end in FIG. 4) 44B 44 and a transmitted light guide 46 for introducing the light transmitted through the absorbance measurement unit 33 and emitted to the photodetector 15 is provided. Further, a suction mechanism hole 48 (see FIG. 5) having a diameter larger than that of the suction mechanism connection hole 38A is formed on the upper surface of the chip holder 40 at a position corresponding to the suction mechanism connection hole 38A of the microchip 30. Yes.
Here, as a specific example of the aperture of the aperture portion 44 and the aperture diameter of the transmitted light guide 46 in the chip holder 40, when the minimum diameter on the light incident / exit surface of the absorptiometry unit 33 is 0.7 mm, The aperture diameter of the aperture 44 is 0.3 mm, and the aperture diameter of the transmitted light guide 46 is 0.5 mm.

As shown in FIG. 5, the microchip measuring apparatus 10 has a temperature control mechanism for controlling the temperature of the microchip 30 in a state where the chip holder 40 is incorporated in the installation space 42 of the chip holder 40. Is provided.
In the example of this figure, the temperature control mechanism is configured to control the temperature of the chip holder 40 and the temperature control means 61 that is provided below the built-in space 42 of the chip holder 40 (downward in FIG. 5), for example, a heater such as a Peltier element. For example, a temperature sensor (not shown in FIG. 5) 62 for measuring, such as a thermistor, is configured, and both the temperature control means 61 and the temperature sensor 62 are connected to the control board 19. Based on the temperature information measured by the above, the temperature control means 61 heats the chip holder 40 itself by entering the heating operation state or cools the microchip 30 by entering the heating operation stop state, for example, The temperature is controlled to be 37 ± 0.5 ° C.

Further, in the microchip measuring apparatus 10, the measurement liquid comprising the reagent mixing unit 37 is connected to the absorbance measurement unit 33 of the microchip 30 on the upper surface (upper surface in FIG. 5) of the microchip 30. A suction mechanism is provided for sucking the liquid to be measured staying in the reservoir part and introducing it into the absorbance measuring part 33.
In the example of this figure, the suction mechanism is provided in a state where it is in contact with the upper surface of the chip holder 40 (hereinafter also referred to as “suction standby state”), and is electrically connected to the chip holder by a driving device 68. 40 is connected to the suction mechanism connection hole 38A of the microchip 30 through the suction mechanism hole 48 (hereinafter also referred to as “suckable state”), for example, a connection portion with the suction mechanism connection hole 38A The O-ring is provided on the stainless steel, and the connection portion comes into close contact with the suction mechanism connection hole 38A, so that the stainless steel is brought into airtight communication with the absorptiometry unit 33 via the suction hole 38 and the suction channel 39D. A suction means 66 made of a pipe made of a metal, and a suction pump 69 mechanically connected to the suction means 66 via a suction pipe 67. It is intended to be introduced into the part for measuring absorbance 33 by moving the analyte solution remaining in the reagent mixing section 37 of the microchip 30 by a Hikitsukuri. The suction means 66 is changed from a suckable state to a suction standby state by the driving device 68 after the absorbance measurement is completed in the absorbance measurement unit 33 of the microchip 30.

As the light source 13, a light emitter that emits light in a continuous wavelength region, specifically, a xenon lamp, a white LED, or the like can be used.
The light source 13 is turned on or off when the light source 27 is controlled by a control signal transmitted from the control board 19, but the state can also be controlled by the operation switch 21.

The optical member 50 preferably forms a linear optical path from the light emitted from the light source 13 to the absorbance measuring unit 33 in the microchip 30.
In the example of this figure, the optical member 50 forms a light source arrangement space 52A for arranging the light source 13, and has a light source holder 52 having an opening 52B on the surface (left surface in FIG. 1) facing the chip holder 40, and The condenser lens 53 is provided in the light source arrangement space 52A of the light source holder 52 and disposed on the optical path from the light emitted from the light source 13 to the opening 52B.

The wavelength selection filter 14 has a high transmittance only for light in a specific wavelength region, and for example, a band pass filter or the like can be used.
The wavelength selection filter 14 can be appropriately selected according to the measurement target liquid introduced into the absorptiometry unit 33 in the microchip 30.

  The photodetector 15 has a function of outputting a light intensity signal based on the received light, and for example, a light receiving element such as a silicon photodiode can be used.

The microchip measuring apparatus 10 having such a configuration operates according to the flowchart shown in FIG.
Specifically, when the power switch 18 is turned on, power is supplied from the commercial AC power source to the microchip measuring apparatus 10 in which the microchip 30 is not inserted and the control board 19 is started. (Step 1) First, the temperature control means 61 and the temperature sensor 62 are driven (Step 2), and a control signal (lighting signal) is transmitted to the light source power source 27 when the control board 19 is started. The light source 13 is turned on when a lighting instruction is given by the operator via the operation switch 21, and the light emitted from the light source 13 is detected through the optical component 50, the wavelength selection filter 14, and the chip holder 40. The light is received by the detector 15 (step 3).
Next, the temperature measured by the temperature sensor 62 (hereinafter also referred to as “sensor measurement temperature”) and the light intensity detected by the photodetector (hereinafter referred to as “detector measurement light intensity”) managed by the control board 19. ”) Both after waiting until they become values suitable for absorbance measurement (step 4), both the sensor measurement temperature and the detector measurement light intensity exceed a specific value (constant value), and the microchip. When the measurement device 10 is ready for absorption spectrophotometry, it is output to the output device 22 that measurement preparation is complete (step 5).

  Then, the operator supplies the test liquid to the test liquid injection part 35 and supplies the reagent to the reagent reservoir 36, and the test liquid and the reagent are mixed in the reagent mixing part 37. The obtained measurement target liquid is not introduced into the absorptiometry unit 33, and the microchip 30 staying in the reagent mixing unit 37 is incorporated into the chip holder 40 (step 6). When a measurement start instruction is indicated by the operator via the switch 21 and the measurement operation is started when the control board 19 receives a measurement start signal from the operation switch 21 (step 7), the drive device 68 The suction means 66 is connected to the suction connection hole 38 </ b> A of the microchip 30, and the measurement target liquid retained in the reagent mixing unit 37 is absorbed by the suction action of the suction pump 69. Is filled is introduced into the time measuring section 33 (Step 8).

Then, after elapse of a standby time set in advance as required, first, the light intensity immediately after the start of measurement (hereinafter also referred to as “initial light intensity”) (I ₀ ) is measured by the photodetector 15 (step S ₀ ). 9) Thereafter, the light intensity (I _t ) is measured at a predetermined time interval (eg, 1 second interval) for a preset measurement time (eg, 10 minutes) (step 10). The measurement of the intensity (I _t ) is finished (step 11).

After the measurement time, the control board 19, first, the initial light intensity I ₀ and the measurement time within the measured absorbance change rate based on the light intensity I _t to be the calculated (step 12), then the calculated change in absorbance Based on the rate, the γ-GTP activity value is calculated (step 13), and the calculation result of the γ-GTP activity value is output to the output device 22 (step 14), thereby being incorporated in the chip holder 40. A series of measurement operations related to the microchip 30 is completed.

Here, the absorbance change rate △ OD between the measurement time t1 and the measurement time t2 is calculated based on the light intensity I _t of the initial light intensity I ₀ and the measurement time t by the following equation (1), the measurement time Based on the absorbance (OD _t1 ) related to _t1 and the absorbance (OD _t2 ) related to measurement time t2, it can be calculated by the following equation (2).

Further, the γ-GTP activity value can be calculated based on a calibration curve between the absorbance change rate and the γ-GTP activity value obtained in advance represented by the following formula (3).
In Formula (3), a and b are each a constant.

  The microchip 30 for which measurement has been completed is taken out by the operator (step 15), and if measurement is to be continued, a new microchip 30 is prepared (step 16), and the new microchip 30 is inserted into the chip holder 40. Measurement can be performed again by repeatedly incorporating the measurement operation.

  Then, the light source 13 is turned off by the operation switch 21 by the operator, the power switch 18 is turned off, and the power from the commercial AC power supply is cut off. The operating state is terminated and the stopped state is entered (step 17).

  In such a microchip measuring device 10, after the measurement preparation during the operation is completed and the fact is output to the output device 22, the temperature control mechanism including the temperature control means 61 and the temperature sensor 62 is operated at the temperature. When the sensor measurement temperature measured by the sensor 62 is below a certain temperature, the temperature control means 61 is in a heating operation state, while the sensor measurement temperature measured by the temperature sensor 62 is above a certain temperature. When the temperature control means 61 is in the heating operation stop state, the chip holder 40 is heated or cooled, and the temperature of the microchip 30 incorporated in the chip holder 40 is controlled by the chemicals to be inspected and the reagent. It is controlled to be in a temperature range suitable for reaction, for example, 37 ± 0.5 ° C.

  According to the microchip measuring apparatus 10 as described above, the temperature of the microchip 30 assembled in the chip holder 40 can be controlled by the temperature control mechanism including the temperature control means 61 and the temperature sensor 62. Since the temperature of the microchip 30 can be set to a temperature suitable for the chemical reaction between the liquid to be inspected and the reagent, the microchip 30 is inspected in a state where the microchip 30 is incorporated in the chip holder 40. The liquid to be measured obtained by mixing the liquid to be inspected supplied to the liquid injection part 35 and the reagent supplied to the reagent reservoir 36 in the reagent mixing part 37 is made to contain a light-absorbing component. In addition, the liquid to be measured obtained in the reagent mixing unit 37 is composed of a suction means 66 and a suction pump 69. The pull mechanism can be reliably introduced into the part for measuring absorbance 33.

  As described above, according to the microchip measuring apparatus 10, the microchip 30 in which the test liquid supplied to the test liquid injection unit 35 and the reagent supplied to the reagent reservoir 36 are mixed in the reagent mixing unit 37. Is incorporated into the chip holder 40, and the liquid to be measured is absorbed by the chemical reaction between the liquid to be inspected and the reagent without moving the microchip built-in body 11 composed of the chip holder 40 with the microchip 30 incorporated therein. A state in which the liquid to be inspected and the reagent are mixed in the reagent mixing unit 37 because the component-containing state, the measurement target liquid can be introduced into the absorptiometry unit 33, and the absorptiometry can be performed. It is not necessary to preheat the microchip 30 before inserting the microchip 30 into the microchip measuring apparatus 10. Since it is not necessary to introduce the pre-measured solution into the absorptiometry unit 33 and fill it in advance, the γ-GTP activity value in the blood, which is the test solution, is finally increased with high measurement efficiency. It can be obtained by spectrophotometric analysis.

  In the microchip measuring apparatus 10, the light emission center of the light source 13, the optical axis of the optical member 50 including the light source holder 52 and the condenser lens 53, the absorbance measuring unit 33 in the microchip 30, and the photodetector 15. Are arranged in a straight line, and the light path from the light emitted from the light source 13 to the light detector 15 is formed in a straight line, so that the light path bent by using various optical members can be obtained. Since the measurement optical system can be easily adjusted as compared with the formed apparatus, and light having a high parallelism can be incident on the absorptiometry unit 33 having a small light incident / exit surface. High measurement accuracy can be obtained.

  The microchip measuring apparatus 10 is provided with a temperature control mechanism and a suction mechanism. Since the temperature control mechanism is provided in the chip holder 40, two mechanisms are provided. Even if it has, it can attain size reduction of the said microchip measuring apparatus 10 itself.

The microchip absorptiometry apparatus of this invention is not limited to what has the said structure, A various change can be added.
For example, the microchip measuring apparatus may have a configuration in which the wavelength selection filter is provided in a replaceable manner. In this case, by using a light emitter that emits light in a continuous wavelength region as a light source, by selecting an appropriate wavelength selection filter, the device is equipped with an LED that emits monochromatic light as a light source. In addition, it is possible to measure the absorbance of a plurality of types of measurement target liquids having different wavelengths of light required for measurement without exchanging the light source. As described above, since the measurement optical system is not greatly displaced, it is possible to easily measure the absorbance of a plurality of types of measurement target liquids with high accuracy.

As a specific example of the microchip measuring device having a configuration in which the wavelength selection filter is replaceable, for example, as shown in FIGS. 7 and 8, a plurality of different characteristics (wavelength ranges of light transmitted with high efficiency are different) A wavelength selective filter exchanging mechanism comprising a rotary filter holder 71 having a wavelength selective filter 14 (six in the example of this figure) and being driven to rotate about a rotary shaft 73 by a stepping motor 72. What is provided is mentioned. The microchip measuring apparatus 70 has the same configuration as the microchip measuring apparatus 10 according to FIG. 1 except that a wavelength selective filter replacement mechanism is provided.
In FIG. 8, the optical path of the light emitted from the light source 13 is shown by a one-dot chain line.

  Further, as shown in FIG. 1, the microchip measuring apparatus is not limited to the one having a configuration in which the wavelength selection filter is provided on the optical path from the light emitted from the light source to the chip built-in body. The transmitted light emitted from the absorptiometry unit may be provided on the optical path to the photodetector, or provided on the optical path formed in the chip holder. The thing of the structure which is may be sufficient.

  In addition, the microchip measuring device includes a light source, an optical member, a wavelength selection filter, and a microchip built-in body, and a measurement region in which an optical path from the light source to the photodetector is formed is shielded by a light shielding plate, for example. It may be configured. In this case, it is possible to prevent a measurement error from occurring due to stray light that is incident on the photodetector due to light that has passed through a portion other than the spectrophotometric measurement portion of the microchip. It is done.

It is the schematic for description which shows an example of a structure of the microchip absorptiometry apparatus of this invention. It is a block diagram for description of the microchip absorptiometry apparatus of FIG. It is explanatory drawing which shows the structure of the microchip which comprises the microchip absorptiometry apparatus of FIG. It is explanatory drawing which shows the structure of the chip | tip holder which comprises the microchip absorptiometry apparatus of FIG. It is the schematic for description which expands and shows the principal part of the microchip absorptiometry apparatus of FIG. It is a flowchart figure which shows the operation state of the microchip absorptiometry apparatus of FIG. It is the schematic for description which shows the other example of a structure of the microchip absorptiometry apparatus of this invention. It is explanatory drawing which shows the structure of the wavelength selection filter exchange mechanism which comprises the microchip absorptiometry apparatus of FIG. It is the schematic for description which shows an example of a structure of the apparatus for analyzing by the conventional microchip analysis system.

Explanation of symbols

10 Microchip spectrophotometer (microchip measuring device)
DESCRIPTION OF SYMBOLS 11 Microchip built-in body 12 Housing | casing 12A Outer peripheral side wall board 12B Front board 13 Light source 14 Wavelength selection filter 15 Photo detector 17 AC inlet 18 Power switch 19 Control board 21 Operation switch 22 Output device 23 External connection terminal 24 Fan 25 Power supply for control 26 Lighting device 27 Power source for light source 28 Terminal block 29 Noise filter 30 Microchip 32 Protruding part 33 Absorbance measuring part 35 Test liquid injection part 35A Air hole 36 Reagent pool part 36A Air hole 37 Reagent mixing part 38 Suction hole part 38A Suction Mechanism connection hole 39A Inspected liquid flow path 39B Reagent flow path 39C Measurement target liquid flow path 39D Suction flow path 40 Chip holder 42 Installation space 42A Chip installation opening 44 Aperture portion 44A One end 44B Other end 46 Transmitted light guide path 48 Suction mechanism Hole 50 optical member 52 light source hole 52A Light source arrangement space 52B Aperture 53 Condensing lens 61 Temperature control means 62 Temperature sensor 66 Suction means 67 Suction pipe 68 Drive device 69 Suction pump 70 Microchip absorptiometry device (microchip measurement device)
71 Rotating Filter Holder 72 Stepping Motor 73 Rotating Shaft 80 Microchip 81 Channel 81A Linear Part 82 Light Source 83 Photodetector

Claims (4)

A microchip having an absorptiometry unit, a chip holder having a chip mounting space in which the microchip is incorporated, an optical member for introducing light emitted from a light source into the absorptiometry unit in the microchip, and a microchip A photodetector that receives the light emitted from the light source that has passed through the absorbance measurement unit of
The microchip is held horizontally by a chip holder,
A microchip absorptiometry apparatus characterized in that the chip holder is provided with a temperature control mechanism for controlling the temperature of the microchip.   The liquid to be measured is formed by communicating with the absorptiometry part on the upper surface of the microchip from the measurement object liquid reservoir part formed in the microchip and communicating with the absorptiometer part. The microchip spectrophotometric measurement apparatus according to claim 1, further comprising a suction mechanism for sucking so as to introduce the liquid.   The light source is composed of a light emitter that emits light in a continuous wavelength range, and a wavelength selection filter is replaceably provided on an optical path from the light emitted from the light source to the photodetector. The microchip absorptiometry apparatus according to claim 1 or 2.   The liquid to be measured is introduced into an absorptiometry unit in a microchip held in a chip holder, and the absorptiometry is performed in a state where the temperature of the microchip is controlled by a temperature control mechanism. The microchip absorptiometry apparatus according to claim 3.

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