CN210394323U - Nucleic acid amplification detection device and system - Google Patents

Abstract

A nucleic acid amplification detection device and a system relate to the field of nucleic acid detection devices. The nucleic acid amplification detection equipment is suitable for being matched with a microfluidic chip for use and comprises a fixed frame, an optical module, a base and a driving mechanism, wherein the fixed frame is provided with a mounting area, and the mounting area is provided with a detection hole which penetrates through the fixed frame and corresponds to a reaction tank of the microfluidic chip; the optical module is used for generating a detection light beam with a preset direction so as to detect the sample and obtain a fluorescence signal; the driving mechanism is fixed on the base and connected with the fixing frame and used for driving the fixing frame to move along a first preset direction and a second preset direction. In the detection process, the microfluidic chip and the fixing frame are kept relatively static, and the sample arranged on the microfluidic chip is also kept relatively static, so that the problem that the conventional problem that the cross contamination between the reaction tanks possibly exists can be solved even if the reaction tanks are mutually communicated. Meanwhile, each reaction cell of the microfluidic chip can be scanned step by step, and the accuracy of data is ensured.

Description

Nucleic acid amplification detection device and system

Technical Field

The application relates to the field of nucleic acid detection equipment, in particular to nucleic acid amplification detection equipment and a nucleic acid amplification detection system.

Background

The micro-fluidic chip technology integrates basic operation units of sample preparation, reaction, separation, detection and the like in the processes of biological, chemical and medical analysis on a micro-fluidic chip with micron scale, and automatically completes the whole analysis process. The microfluidic chip for microfluidic detection generally has the advantages of less sample consumption, high detection speed, simple and convenient operation, multifunctional integration, small volume, convenience in carrying and the like, so that the microfluidic chip is particularly suitable for developing bedside (POC) diagnosis and has great potential for simplifying the diagnosis process and improving the medical result.

Nucleic acid amplification is a general term for a large class of technical methods, and currently comprises conventional PCR (temperature-variable amplification method), real-time fluorescence PCR, isothermal amplification and color development detection, isothermal nucleic acid amplification technology and the like. The current nucleic acid amplification detection equipment has the characteristics of low flux, large volume, complex operation, long detection time, high price and the like.

The existing nucleic acid amplification detection equipment has the defects that reaction tanks of a microfluidic chip are communicated, and cross contamination between the reaction tanks possibly exists along with the action of centrifugation and heating reaction.

SUMMERY OF THE UTILITY MODEL

The present application provides a nucleic acid amplification detection apparatus and system to improve the above-mentioned problems.

The nucleic acid amplification detection device according to the embodiment of the first aspect of the application is suitable for being matched with a microfluidic chip. The nucleic acid amplification detection device comprises a fixing frame, an optical module, a base and a driving mechanism.

The fixing frame is defined with a first preset direction and a second preset direction which are perpendicular to each other, the fixing frame is provided with a mounting area for mounting the microfluidic chip, and the mounting area is provided with a detection hole which penetrates through the fixing frame and corresponds to a reaction tank of the microfluidic chip. The optical module is used for generating a detection light beam with a preset direction so as to detect the sample and obtain a fluorescence signal. The driving mechanism is fixed on the base and connected with the fixing frame and used for driving the fixing frame to move along a first preset direction and a second preset direction so that the detection hole passes through the detection light beam.

According to the nucleic acid amplification detection equipment based on the microfluidic chip, the existing centrifugal mode (centrifugal mode can generate centrifugal force) is replaced by the mode that the fixing frame moves linearly along the first preset direction and the second preset direction, the microfluidic chip and the fixing frame are kept relatively static, and a sample in the reaction tank is kept in a relatively static state, so that the problem that the existing' defect of cross contamination between the reaction tanks can be overcome even if the reaction tanks of the microfluidic chip are communicated at the moment is solved. Meanwhile, each reaction cell of the microfluidic chip can be scanned step by step, so that the data accuracy of each reaction cell can be ensured. The optical module is used for generating a detection light beam which is preset to point, namely, the optical module is in a fixed state in the whole detection process, the stability of a fluorescence signal is guaranteed, and the data accuracy of each reaction cell is further guaranteed by matching with a moving mode.

In addition, the nucleic acid amplification detection apparatus according to the embodiment of the present application has the following additional technical features:

in connection with the first aspect, the present application illustrates some embodiments wherein the drive mechanism includes a first drive mechanism and a second drive mechanism.

Wherein, first actuating mechanism includes: the first fixed plate, the first slider that is connected with the mount and set up in the first step motor of first fixed plate, first step motor is connected with the transmission of first slider for drive first slider is along first predetermined direction reciprocating motion.

The second drive mechanism includes: the second fixed plate is fixed on the base, the second sliding block is connected with the first fixed plate, and the second stepping motor is arranged on the second fixed plate and is in transmission connection with the second sliding block and used for driving the second sliding block to reciprocate along a second preset direction.

Through the setting of first actuating mechanism and second actuating mechanism, realize actuating mechanism drive mount along the function of first direction of predetermineeing and the straight-line motion of second direction of predetermineeing, can realize the unsettled setting of mount simultaneously, guarantee that the inspection hole can be through the detection light beam and guarantee the stationarity of the in-process that removes.

Optionally, the first driving mechanism includes a limiting plate, a motor driving plate, a photoelectric switch, and a limiting plate.

The limiting plate is fixed on the first fixing plate, the first stepping motor is fixed on the limiting plate, and the motor driving plate is connected with the first stepping motor and controls the movement of the first stepping motor; the photoelectric switch is arranged on the limiting plate and forms a photoelectric switch induction area used for obtaining an optical signal, and the photoelectric switch is connected with the motor driving plate; the limiting piece is arranged on the first sliding block and protrudes out of the first sliding block, so that when the first sliding block abuts against the limiting plate, the limiting piece is located in the photoelectric switch sensing area and shields the optical signal.

Through the cooperation of first photoelectric switch and spacing piece, when spacing piece is located the photoelectric switch induction zone and shelters from optical signal, the initial position that first slider of first photoelectric switch discernment and limiting plate leaned on, at this moment, first photoelectric switch will discern the information transmission of initial position to first motor drive board, the first biggest stroke of predetermined direction is confirmed to the step number of first step motor of first motor drive board control, realize the reciprocating motion of first slider in first predetermined direction, progressively scan the reaction cell, cooperation through first photoelectric switch and spacing piece simultaneously, the stroke that can accurate control removed, prevent to make the too big first slider and the limiting plate striking that leads to of removal stroke because of unable discernment initial position, the impact influences the reaction cell in the sample production cross contamination's between the reaction cell that violent removal probably leads to drawback.

In some embodiments, the optical module includes an excitation light source, an emission light path, a receiving light path, and a detector for receiving fluorescence transmitted by the light path to obtain a fluorescence signal.

Wherein, the emission light path includes: the device comprises a first condenser, an excitation optical filter, a first convex lens, a second convex lens, a dichroic mirror and a front focusing mirror which are arranged in sequence; the first condenser lens is used for receiving exciting light emitted by the exciting light source, collimating the exciting light and then emitting the collimated exciting light to the exciting light filter, the exciting light filter filters the exciting light into quasi-monochromatic light, the quasi-monochromatic light is focused again through the first convex lens to become parallel light, the parallel light passes through the second convex lens to obtain a collimated light spot, the collimated light spot is reflected to the front condenser lens through the dichroic mirror and is focused through the front condenser lens to form a detection light beam.

Optionally, the receiving optical path includes a reflecting mirror, a fluorescent filter and a rear focusing mirror, which are sequentially arranged; the reflecting mirror is used for receiving fluorescence generated by the reaction tank under the excitation of the detection light beam and processed by the front focusing mirror and the dichroic mirror in sequence, and the fluorescence reflected by the reflecting mirror is focused on a receiving window of the detector through the rear focusing mirror after being filtered by the fluorescence filter.

The emission light path and the receiving light path are limited, so that the detection light beam with the preset direction can be generated to detect the sample, the fluorescence signal is obtained, the structure of the system is simplified under the condition of meeting the requirement of higher resolution, and the object-image distance is compressed. The emission light path can realize collimation, thereby simplifying the front and rear focusing lenses.

In combination with the first aspect, in some embodiments shown in the present application, the nucleic acid amplification detection apparatus includes a temperature control mechanism, the temperature control mechanism has a heating surface, and the temperature control mechanism is disposed at a distance from the mounting region, so that the heating surface and the mounting region together form a cavity for accommodating the microfluidic chip, and when the microfluidic chip is mounted in the mounting region, the heating surface can contact with a side of the microfluidic chip away from the reaction cell for heating.

The mode of contact heating not only makes heating efficiency higher, simultaneously, compares in the mode of direct contact heating reaction cell (here also including heating reaction channel) or air heating, what this application adopted utilizes heating surface heat conduction heating micro-fluidic chip earlier, and micro-fluidic chip utilizes the heat conduction heating reaction cell that it contains, consequently more even to the heating of reaction cell, has guaranteed the uniformity of the temperature of the reaction cell on the micro-fluidic chip.

In combination with the first aspect, the present application illustrates some embodiments in which the temperature control mechanism includes a heating unit fixed to the fixing frame, the heating unit includes a first heating block having a heating surface, a second heating block, and a cooling plate.

Wherein, one side of the first heating block departing from the heating surface is provided with a first groove; the second heating block and the first heating block are in heat conduction connection and mutually attached, so that the first groove and the first heating block form an installation cavity; the refrigeration piece is installed in the installation intracavity, and the cold junction and the laminating of second heating piece and heat conduction of refrigeration piece are connected, and the hot junction and the laminating of first heating piece and heat conduction of refrigeration piece are connected.

Optionally, the temperature control mechanism further includes a temperature sensor, and the temperature sensor is partially or completely disposed on the first heating block.

Through the arrangement of the temperature sensor, the temperature of the heating surface can be obtained in real time, and the heating temperature of the refrigerating sheet can be conveniently controlled.

Optionally, the temperature control mechanism further comprises a temperature protection switch, part or all of the temperature protection switch is arranged on the first heating block, and the refrigeration sheet is electrically connected with the power supply through the temperature protection switch.

Through the setting of temperature protection switch, when the temperature of first heating piece and second heating piece was too high, disconnection refrigeration piece and power prevented the high temperature.

In some embodiments illustrated herein in combination with the first aspect, the nucleic acid amplification detection apparatus comprises: the display screen is connected with the processor;

the processor is connected with the optical module and used for receiving the fluorescence signal, generating a real-time fluorescence detection signal and displaying the real-time fluorescence detection signal through the display screen, and the processor is connected with the driving mechanism and used for controlling the driving mechanism to move along a first preset direction and a second preset direction.

According to a nucleic acid amplification detection system of the embodiment of the second aspect of the present application, the nucleic acid amplification detection system comprises a microfluidic chip and the nucleic acid amplification detection device provided by the embodiment of the first aspect of the present application, the microfluidic chip is mounted on a fixing frame, and reaction cells of the microfluidic chip are arranged along a first preset direction and/or a second preset direction.

According to the nucleic acid amplification detection system of the embodiment of the application, by using the nucleic acid amplification detection device, when the nucleic acid amplification detection device moves linearly along the first preset direction and the second preset direction, the microfluidic chip and the fixing frame keep relatively static, so that even if the reaction chambers of the microfluidic chip are communicated, the problem of the existing 'defect of cross contamination between the reaction chambers possibly' can be reduced. Meanwhile, each reaction cell of the microfluidic chip can be scanned step by step, so that the data accuracy of each reaction cell can be ensured.

Additional aspects and advantages of the present application will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the present application.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained from the drawings without inventive effort.

FIG. 1 is a schematic diagram showing a first perspective view of a nucleic acid amplification detecting apparatus according to example 1 of the present application;

FIG. 2 is an exploded view of the nucleic acid amplification detecting apparatus provided in example 1 of the present application;

FIG. 3 is a schematic view of the nucleic acid amplification detecting apparatus provided in example 1 of the present application;

fig. 4 is a schematic structural view of a fixing frame provided in embodiment 1 of the present application;

fig. 5 is an optical schematic diagram of an optical module provided in embodiment 1 of the present application;

fig. 6 is a schematic structural diagram of an optical module provided in embodiment 1 of the present application;

fig. 7 is a schematic structural view of a driving mechanism provided in embodiment 1 of the present application;

fig. 8 is an exploded view of a first drive mechanism provided in embodiment 1 of the present application;

fig. 9 is an exploded view of a temperature control mechanism provided in embodiment 1 of the present application;

fig. 10 is an exploded view of a heating unit provided in embodiment 1 of the present application;

FIG. 11 is a schematic diagram of a second perspective view of the nucleic acid amplification detecting apparatus provided in example 1 of the present application.

Icon: 10-a nucleic acid amplification detection device; 100-a fixing frame; 101-a second groove; 103-a mounting area; 104-a detection well; 105-a first opening; 106-positioning fixed structure; 1061-positioning protrusions; 1063-spring plate; 1065-a carrying tank; 110-an optical module; 111-excitation light source; 1131 — first condenser; 1133 — excitation filter; 1135 — first convex lens; 1136 — second convex lens; 1137-dichroic mirror; 1138, front focusing mirror; 1151-mirror; 1153-a fluorescent filter; 1155-rear focusing mirror; 117-a detector; 118-an optical body box; 120-a drive mechanism; 121-a first drive mechanism; 1211 — a first fixing plate; 1212-first slider; 1214-a first stepper motor; 1215-the optical axis; 1217-bearing fixing plate; 1218-synchronizing wheel; 1219-conveyor belt; 1220-motor fixing plate; 1221-linear bearings; 1223-pressing plate; 1224-limiting plate; 1225-motor drive board; 1226-photoelectric switch; 1227-a limiting piece; 1228-photoelectric switch sensing area; 123-a second drive mechanism; 1231-a second fixation plate; 1233-a second slider; 1235-second stepper motor; 130-temperature control mechanism; 131-a heating unit; 1311-first heating block; 1313-a second heating block; 1315-refrigeration piece; 1316-a first groove; 133-a temperature sensor; 1331-a first mounting groove; 134-temperature protection switch; 1341-a second mounting groove; 135-a first heat dissipation group; 1351-first fan case; 1353-an air inlet; 1355-first fan; 137-a second heat dissipation group; 1371-a second fan case; 1373-a second fan; 140-a power supply filter; 150-a housing; 151-upper shell wall; 1531-a base; 1533-a first side panel; 154-heat dissipation holes; 155-USB interface; 156-network port interface; 157-an elastomeric buffer; 158-a second opening; 159-hatch door; 160-a processor; 171-a scaffold; 170-display screen; 20-microfluidic chip.

Detailed Description

Reference will now be made in detail to the embodiments of the present application, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary only for the purpose of explaining the present application and are not to be construed as limiting the present application.

Example 1

Referring to fig. 1, 2 and 3, the present application provides a nucleic acid amplification detection apparatus 10, which is used in cooperation with a microfluidic chip 20 to perform nucleic acid amplification detection based on the microfluidic chip 20, so as to avoid the complicated operation of frequently opening a cover of a PCR tube in the conventional nucleic acid amplification process, shorten the detection time, reduce the detection cost, and effectively reduce the pollution.

Nucleic acid amplification detection apparatus 10 includes a holder 100, an optical module 110, a base 1531, a drive mechanism 120, a processor 160, and a display screen 170 connected to processor 160.

Wherein the holder 100 is used for mounting the microfluidic chip 20.

Specifically, referring to fig. 5, the fixing frame 100 defines a first predetermined direction (denoted by X) and a second predetermined direction (denoted by Y) perpendicular to each other, the fixing frame 100 is disposed with a mounting area 103 for mounting the microfluidic chip 20, and the mounting area 103 has a detection hole 104 penetrating through the fixing frame 100 and corresponding to a reaction cell of the microfluidic chip 20.

It should be noted that the first predetermined direction and the second predetermined direction are perpendicular to each other. Meanwhile, for convenience of description, the fixing frame 100 defines a third predetermined direction (denoted by Z), the third predetermined direction, the first predetermined direction and the second predetermined direction are perpendicular to each other, and at this time, the detecting hole 104 penetrates through the fixing frame 100 along the third predetermined direction.

The projection of the fixing frame 100 on the horizontal plane is, for example, rectangular.

Optionally, the fixing frame 100 has a top surface and a bottom surface opposite to each other along a third predetermined direction, where the top surface is a side of the fixing frame 100 away from the base 1531 in the use state. The top surface is provided with a second groove 101, wherein the mounting region 103 is disposed on the bottom wall of the second groove 101. The number of the mounting regions 103 may be one or more, when the number of the mounting regions 103 is multiple, the plurality of mounting regions 103 are arranged at intervals along the second preset direction, and optionally, each mounting region 103 is in a strip shape, and each strip-shaped mounting region 103 extends along the first preset direction.

In order to facilitate the installation of the microfluidic chip 20 in the mounting region 103, two sidewalls of the second groove 101 along the first predetermined direction or a sidewall deviating from the first side plate 1533 are provided with a first opening 105, the first opening 105 is communicated with the second groove 101, and the microfluidic chip 20 can be installed on or removed from the mounting region 103 through the first opening 105.

Optionally, with continued reference to fig. 4, the mounting area 103 is provided with a positioning fixing structure 106, and the positioning fixing structure 106 includes a positioning protrusion 1061 and a spring plate 1063. The microfluidic chip 20 is provided with a positioning groove matched with the positioning protrusion 1061, so as to effectively position and guide the position, and ensure that the detection hole 104 corresponds to the position of the reaction cell. The positioning protrusion 1061 is, for example, a snap spring.

The elastic sheet 1063 can contract and expand along the first preset direction under the action of a force in the first preset direction, and deform in the third preset direction to bear the microfluidic chip 20 and provide an upward thrust to the microfluidic chip 20, wherein the elastic sheet 1063 is arc-shaped and protrudes to one side away from the mounting area 103, so that the microfluidic chip 20 can be inserted and pulled out through the first opening 105, and is convenient to mount and dismount.

Optionally, the mounting area 103 is provided with a bearing groove 1065, the elastic sheet 1063 is embedded in the bearing groove 1065, a first end of the elastic sheet 1063 along the first preset direction is fixed to the bearing groove 1065, a second end of the elastic sheet 1063 is slidably disposed in the bearing groove 1065, and the elastic sheet 1063 protrudes out of the bearing groove 1065 for bearing the microfluidic chip 20, wherein when the elastic sheet 1063 is in a natural state, a movable gap is formed between the second end and the bearing groove 1065 for providing a space for the second end to move to a side away from the first end.

The number of the reaction cells of the microfluidic chip 20 may be multiple, and the reaction cells of the microfluidic chip 20 are arranged along a first preset direction and/or a second preset direction. For example, a plurality of reaction tanks are arranged at intervals along a first preset direction; or a plurality of reaction tanks are arranged at intervals along a second preset direction; or a plurality of reaction tanks are arranged at intervals along a first preset direction and a second preset direction, for example, the reaction tanks are arranged in a rectangular array.

Wherein, the detection hole 104 can correspond with the reaction tank one-to-one, also can be a plurality of reaction tanks of detection hole 104 correspondence, for example, the quantity of detection hole 104 corresponds with micro-fluidic chip 20's quantity, this moment, this detection hole 104 extends along the direction of arranging of a plurality of reaction tanks, for example, arrange along first preset direction interval when a plurality of reaction tanks, detection hole 104 is the bar hole, the length direction of the cross-section in bar hole is parallel with first preset direction simultaneously, and then when this micro-fluidic chip 20 was installed in installing zone 103, this micro-fluidic chip 20's reaction tank all was located the detection hole 104's that this micro-fluidic chip 20 corresponds within range, make the detection beam through detection hole 104 can shine each reaction tank.

Meanwhile, in order to avoid cross contamination between the reaction cells, the microfluidic chip 20 may include a plurality of independent channels, each channel is provided with a reaction cell, and a primer is embedded in the reaction cell.

For example, the microfluidic chip 20 has 32 reaction cells pre-embedded with primers, and the primers are standard quality control index nucleic acid probes capable of generating fluorescent signals and used for indicating the effectiveness of the nucleic acid specificity detection and identification result. Aiming at different detection requirements, the number of the reaction pools with the pre-embedded primers can be customized according to different detection items, and the primers can also be set according to actual requirements. For ease of operation, the sample inlets of each channel may optionally be pooled in a total sample inlet.

The fluorescence method has high detection sensitivity, and therefore, in the present application, the nucleic acid amplification detection apparatus 10 performs real-time detection on nucleic acid amplification by using real-time fluorescence, wherein the optical module 110 is configured to generate a detection light beam with a preset direction to detect a sample and obtain a fluorescence signal, in other words, the position of the optical module 110 is kept fixed, so as to ensure stability of the fluorescence signal and improve detection accuracy. The sample is a reaction pool embedded with primers.

Specifically, the optical module 110 is fixed on the base 1531, and is used to ensure that the optical module 110 is in a fixed state in the whole detection process, so as to ensure the stability of the fluorescence signal.

Referring to fig. 1, 5 and 6, the optical module 110 includes: an excitation light source 111, an emission light path, a receiving light path, and a detector 117 for receiving the fluorescence transmitted by the light path to obtain a fluorescence signal.

The excitation light source 111 is connected to the processor 160, and the processor 160 controls the excitation light source 111 to emit excitation light. The excitation light source 111 is a cold light source, such as an LED light source, so as to avoid heat accumulation, and at the same time, no additional fan is required for heat dissipation.

The detector 117 is coupled to the processor 160 for receiving the fluorescence signal and generating a real-time fluorescence detection signal, and the processor 160 is coupled to the display screen 170 so that the real-time fluorescence detection signal obtained by the processor 160 can be displayed by the display screen 170.

The detector 117 is a photomultiplier tube, and the photomultiplier tube is used for receiving fluorescent signals, so that the detector has the characteristics of extremely high sensitivity and high response speed, and the application of the photomultiplier tube ensures the precision and reliability of the device. Since the photomultiplier is a precise optical element, fixing the detector 117 to the base 1531 ensures that the detector is in a stationary state during the entire detection process, and ensures the stability of the detector 117 in receiving the fluorescence signal.

Optionally, referring to fig. 5, the emission optical path includes: a first condenser 1131, an excitation filter 1133, a first convex lens 1135, a second convex lens 1136, a dichroic mirror 1137, and a front focusing mirror 1138, which are sequentially disposed. The first condenser 1131 is configured to receive excitation light emitted by the excitation light source 111, collimate the excitation light, and emit the collimated excitation light to the excitation filter 1133, the excitation filter 1133 filters the excitation light into quasi-monochromatic light, and focuses the quasi-monochromatic light again through the first convex lens 1135 to become parallel light, the parallel light passes through the second convex lens 1136 to obtain a collimated light spot, and the collimated light spot is reflected to the front condenser 1138 through the dichroic mirror 1137 and is focused through the front condenser 1138 to form a detection light beam. The receiving optical path includes: a reflector 1151, a fluorescence filter 1153 and a rear focusing mirror 1155 which are arranged in sequence; the reflecting mirror 1151 is used for receiving fluorescence generated by the reaction cell under excitation of the detection light beam and processed by the front focusing mirror 1138 and the dichroic mirror 1137 in sequence, and the fluorescence reflected by the reflecting mirror 1151 is filtered by the fluorescence filter 1153 and then focused on the receiving window of the detector 117 by the rear focusing mirror 1155.

The emission light path can realize collimation, the detection light beam with preset direction can be ensured to be generated to detect the sample through the limitation of the emission light path and the receiving light path, the fluorescence signal is obtained, the structure of the system is simplified under the condition of meeting the requirement of higher resolution ratio, and the object-image distance is compressed.

The optical module 110 further includes an optical body box 118, and the transmitting optical path and the receiving optical path are disposed in the optical body box 118, and are configured to obtain the detection light beam with the preset direction.

Referring to fig. 2 and fig. 6, the optical body box 118 is fixed to the base 1531 by bolts, for example, and the excitation light source 111 and the detector 117 are both connected to the optical body box 118, wherein the detector 117 is disposed on a side of the excitation light source 111 close to the base 1531, and a gap is formed between the detector 117 and the base 1531, that is, the detector 117 is connected to the base 1531 through the optical body box 118 and is suspended above the base 1531, so as to save space, on the one hand, and reduce a problem of inaccurate measurement result caused by displacement of the detector 117 due to vibration of the base 1531 compared with the case where the detector 117 is directly connected to the base 1531. In fig. 6, the direction of the light is indicated by an arrow.

In summary, the fixing frame 100 is suspended and located above the optical module 110 (at a side of the optical module 110 away from the base 1531), so that the detection light beam passes through the detection hole 104 from bottom to top (from a side close to the base 1531 to a side close to the upper housing wall 151) along a third predetermined direction.

It should be noted that whether the micro-fluidic chip 20 is mounted on the fixing frame 100 can be determined by the signal value of the optical path obtained by the optical module 110, where the signal value is small when there is no micro-fluidic chip 20 and is large when there is a micro-fluidic chip 20.

The driving mechanism 120 is used for connecting with the fixing frame 100, ensuring that the fixing frame 100 is suspended and located above the optical module 110, and ensuring that the detection light beam can be sent to the primers in the reaction cell through the detection hole 104.

Specifically, the driving mechanism 120 is fixed to the base 1531 and connected to the fixing frame 100, and is configured to drive the fixing frame 100 to move along a first preset direction and/or a second preset direction, so that the detection hole 104 passes through the detection light beam.

The fixing frame 100 is adopted to move along the first preset direction and the second preset direction to replace the existing centrifugal mode, and the microfluidic chip 20 and the fixing frame 100 are kept relatively static, so that even if the reaction cells of the microfluidic chip 20 are communicated at the moment, the problem of the existing 'defect of cross contamination possibly existing between the reaction cells' can be reduced due to the lack of the action of centrifugal force. Meanwhile, each reaction cell of the microfluidic chip 20 can be scanned step by step, so that the accuracy of data of each reaction cell can be ensured.

Referring to fig. 7 and 8, the driving mechanism 120 includes a first driving mechanism 121 and a second driving mechanism 123.

The first drive mechanism 121 includes: the first fixed plate 1211, a first slider 1212 connected to the fixed frame 100, and a first stepping motor 1214 disposed on the fixed plate, wherein the first stepping motor 1214 is in transmission connection with the first slider 1212, and is configured to drive the first slider 1212 to reciprocate along a first preset direction; the second drive mechanism 123 includes: a second fixing plate 1231 fixed to the base 1531, a second sliding block 1233 connected to the first fixing plate 1211, and a second stepping motor 1235 disposed on the second fixing plate 1231, wherein the second stepping motor 1235 is in transmission connection with the second sliding block 1233, and is configured to drive the second sliding block 1233 to reciprocate along a second predetermined direction.

Through the arrangement of the first driving mechanism 121 and the second driving mechanism 123, the function of the driving mechanism 120 driving the fixing frame 100 to move along the first preset direction and the second preset direction is realized. In other words, the first driving mechanism 121 and the second driving mechanism 123 are distributed along the third predetermined direction and have a certain height, so as to realize the suspension of the fixing frame 100.

The first driving mechanism 121 and the second driving mechanism 123 are arranged in the same manner, so that only the specific structure of the first driving mechanism 121 is described in detail below, and the specific structure of the second driving mechanism 123 is not described herein again.

Specifically, referring to fig. 7 and 8, the first driving mechanism 121 further includes an optical axis 1215, a bearing fixing plate 1217, a synchronizing wheel 1218, and a motor fixing plate 1220.

The bearing fixing plate 1217 and the motor fixing plate 1220 are disposed on the first fixing plate 1211 at an interval along a first predetermined direction, and the bearing fixing plate 1217 and the motor fixing plate 1220 are respectively disposed on a side of the first fixing plate 1211 facing away from the bearing plate, wherein the first stepping motor 1214 is disposed on the motor fixing plate 1220, the synchronizing wheel 1218 is rotatably disposed on the bearing fixing plate 1217, and the first stepping motor 1214 and the synchronizing wheel 1218 are connected to each other by a transmission belt 1219, so that the synchronizing wheel 1218 and the first stepping motor 1214 rotate synchronously. The synchronizing wheel 1218 may also be replaced by a bearing rotatably disposed on the bearing fixing plate 1217, which is not described herein.

The optical axis 1215 is disposed along a first predetermined direction, one end of the optical axis 1215 is connected to the bearing fixing plate 1217, the other end of the optical axis 1215 is connected to the motor fixing plate 1220, and the first slider 1212 is slidably disposed on the optical axis 1215, so that the first slider 1212 can move linearly along the first predetermined direction. The number of the optical axes 1215 may be one or more, and is not limited herein.

The first slider 1212 is slidably disposed on the optical axis 1215 in the following manner: the first block 1212 is slidably disposed on the optical axis 1215 through the linear bearing 1221, or the first block 1212 is directly sleeved on the optical axis 1215.

The first slider 1212 is fixed to the conveyor belt 1219, so that the first slider 1212 and the conveyor belt 1219 move synchronously, and the first slider 1212 is driven by the conveyor belt 1219 to perform a reciprocating linear motion along a first preset direction. The first slider 1212 may be fixed to the conveyor belt 1219 by, for example, pressing the conveyor belt 1219 to the first slider 1212 with a pressing plate 1223, or may be directly fixed to the conveyor belt 1219 by, for example, gluing the first slider 1212.

In order to reduce the friction force, a certain gap is formed between the first slider 1212 and the first fixing plate 1211, that is, the first slider 1212 is suspended from the first fixing plate 1211.

In addition to the above components, the first driving mechanism 121 optionally further includes: limiting plate 1224, motor drive board 1225, photoelectric switch 1226 and spacing piece 1227.

The limiting plate 1224 is fixed to the first fixing plate 1211, and the first stepping motor 1214 is fixed to the limiting plate 1224; the motor drive board 1225 is connected to the first stepping motor 1214, and controls the movement of the first stepping motor 1214, where the movement includes, for example, controlling the number of steps and the stroke of the first stepping motor 1214; the photoelectric switch 1226 is arranged on the position limiting plate 1224 and forms a photoelectric switch 1226 sensing area for obtaining a light signal, and the photoelectric switch 1226 is connected with the motor driving plate 1225; the limiting piece 1227 is disposed on the first sliding block 1212 and protrudes from the first sliding block 1212, so that when the first sliding block 1212 abuts against the limiting plate 1224, the limiting piece 1227 is located in the sensing area of the optoelectronic switch 1226 and blocks the optical signal.

The motor driving board 1225 is connected to the controller, and is used for controlling the first driving mechanism 121 to move along a first preset direction.

The photoelectric switch 1226 is used to detect whether the limiting piece 1227 exists or not, and when the limiting piece 1227 is located in the sensing area of the photoelectric switch 1226 and blocks the optical signal, the existence of the limiting piece 1227 is detected, and the setting mode can refer to the related technology. Through the cooperation of photoelectric switch 1226 and spacing piece 1227, when spacing piece 1227 is located photoelectric switch 1226 induction area and shelters from optical signal, photoelectric switch 1226 detects the existence of spacing piece 1227, also be the initial position that first slider 1212 supports with limiting plate 1224 and support, at this moment, photoelectric switch 1226 will discern the information transmission of initial position to motor drive board 1225, the biggest stroke of first preset direction is confirmed to the step number of motor drive board 1225 control first step motor 1214, realize the reciprocating motion of first slider 1212 in first preset direction.

Optionally, the optoelectronic switch 1226 is disposed on a side of the limiting plate 1224 away from the base 1531, where the optoelectronic switch 1226 may be a correlation optoelectronic switch 1226, or a slit optoelectronic switch 1226, for example, U-shaped, C-shaped, or the like. In this embodiment, a U-shaped slit optoelectronic switch 1226 is adopted, and at this time, the sensing area of the optoelectronic switch 1226 refers to a slit between the emitter and the receiver of the U-shaped slit optoelectronic switch 1226, wherein the limiting piece 1227 is driven by the first stepping motor 1214 to be inserted into the slit along a first preset direction without contacting the optoelectronic switch 1226, so as to shield the optical signal.

The nucleic acid amplification is performed under suitable temperature conditions, and thus, the temperature control mechanism 130 is used to ensure a temperature required for the nucleic acid amplification reaction.

The temperature control mechanism 130 has a heating surface, the temperature control mechanism 130 and the mounting region 103 are arranged at an interval, so that the heating surface and the mounting region 103 together form a cavity for accommodating the microfluidic chip 20, and when the microfluidic chip 20 is mounted on the mounting region 103, the heating surface can contact with one side of the microfluidic chip 20 away from the reaction cell for heating.

The mode of contact heating not only makes heating efficiency higher, simultaneously, compares in the mode of direct contact heating reaction cell (here also including heating reaction channel), what this application adopted is that the heat conduction of heating face is utilized earlier to heat micro-fluidic chip 20, and micro-fluidic chip 20 utilizes the heat conduction heating reaction cell that it contains, consequently more even to the heating of reaction cell, has guaranteed the uniformity of the temperature of the reaction cell on the micro-fluidic chip 20. Meanwhile, the elastic sheet 1063 is arranged to ensure the close contact between the microfluidic chip 20 and the heating surface, thereby improving the heat conduction efficiency.

Optionally, referring to fig. 2, 9 and 10, the temperature control mechanism 130 includes a heating unit 131 fixed to the fixing frame 100, and the heating unit 131 includes a first heating block 1311, a second heating block 1313 and a cooling plate 1315.

The first heating block 1311 has a heating surface, and a first groove 1316 is formed in a side, away from the heating surface, of the first heating block 1311; the second heating block 1313 and the first heating block 1311 are in heat conduction connection and attached to each other, so that the first recess 1316 and the first heating block 1311 form a sealed installation cavity; the refrigeration piece 1315 is installed in the installation cavity, the cold end of the refrigeration piece 1315 is attached to the second heating block 1313 and is in heat conduction connection, and the hot end of the refrigeration piece 1315 is attached to the first heating block 1311 and is in heat conduction connection.

Meanwhile, the heating unit 131 is located on a side of the fixing frame 100 away from the base 1531 along a third predetermined direction.

The fixing of the heating unit 131 to the fixing frame 100 can ensure the stability of the contact between the heating surface and the microfluidic chip 20 and the stability of contact heating during the heating process.

Wherein, the heating unit 131 may be fixed to the fixing frame 100 by bolts or an adhesive using the first heating block 1311. Optionally, the second heating block 1313 is fixed to the top surface of the fixing frame 100 by bolts, the first heating block 1311 is embedded in the first recess 1316, and in order to ensure the heating effect, the first heating block 1311 is tightly embedded in the first recess 1316, so that the heating surface and the first recess 1316 form a mounting cavity.

The first heating block 1311 and the second heating block 1313 may be bonded by a heat conductive adhesive, or may be connected by a bolt, so as to ensure the connection stability of the two blocks.

Optionally, the cooling fins 1315 are connected to a controller, which controls the heating temperature of the cooling fins 1315.

Optionally, the temperature control mechanism 130 further includes a temperature sensor 133, and a part or all of the temperature sensor 133 is disposed on the first heating block 1311. The temperature of the heating surface can be obtained in real time by the arrangement of the temperature sensor 133, so that the heating temperature of the cooling fins 1315 can be controlled conveniently. Wherein, the temperature sensor 133 is connected with the controller, and the controller obtains the temperature and displays the temperature on the display screen 170, which is convenient for direct obtaining.

A first mounting groove 1331 is formed at a connection portion of the first heating block 1311 and the second heating block 1313, and the temperature sensor 133 is embedded in the first mounting groove 1331, that is, the temperature sensor 133 is partially disposed in the first heating block 1311.

Optionally, the temperature control mechanism 130 further includes a temperature protection switch 134, part or all of the temperature protection switch 134 is disposed on the first heating block 1311, and the refrigeration sheet 1315 is electrically connected to a power supply (not shown) through the temperature protection switch 134.

Optionally, a second installation groove 1341 is formed at a connection portion of the first heating block 1311 and the second heating block 1313, and the temperature protection switch 134 is embedded in the second installation groove 1341, that is, the temperature sensor 133 is partially disposed in the first heating block 1311.

By setting the temperature protection switch 134, when the temperatures of the first heating block 1311 and the second heating block 1313 are too high, the cooling plate 1315 is disconnected from the power supply, and the temperature is prevented from being too high.

It should be noted that the power source may be a storage battery, and in this embodiment, the power source is an external power source, so referring to fig. 11, the nucleic acid amplification detecting apparatus 10 is provided with a socket (not shown) and a power filter 140 connected to the socket via a wire, the power filter 140 is disposed on the base 1531, and the power filter 140 is electrically connected to the processor 160, the display screen 170, the driving mechanism 120, the optical module 110, and the temperature control mechanism 130, and supplies power to each component through the external power source.

Optionally, with continued reference to fig. 2, 9, and 10, the temperature control mechanism 130 includes a first heat dissipation group 135 and a second heat dissipation group 137.

The first heat dissipation assembly 135 includes a first fan housing 1351 and a first fan 1355, wherein the first fan housing 1351 is connected to the top surface of the fixing frame 100, and an installation space is formed between the first fan housing 1351 and the fixing frame 100, and the heating unit 131 is located in the installation space and has an air gap with the first fan housing 1351, in other words, each component in the heating unit 131 is not in contact with the first fan housing 1351.

The side of the first fan housing 1351 facing away from the fixed frame 100 is provided with an air inlet 1353, and the air inlet 1353 is communicated with the installation space. The first fan 1355 is mounted on a side of the first fan housing 1351 facing away from the second heating block 1313, and when the first fan 1355 is rotated, the generated wind is blown to the second heating block 1313 through the wind inlet 1353 to dissipate heat.

The second heat dissipation assembly 137 includes a second fan housing 1371 and a second fan 1373, wherein the second fan housing 1371 is connected to the base 1531, a cavity is formed between the second fan housing 1371 and the base 1531, and the second fan 1373 is installed at a side of the second fan housing 1371 away from the base 1531, wherein an air opening is provided at a side of the second fan housing 1371 away from the base 1531, in other words, the second fan 1373 is installed at the second fan housing 1371 and located at the air opening, and is configured to blow generated air to a side of the fixing frame 100 close to the base 1531 to dissipate heat when the second fan 1373 rotates. The base 1531 may have a heat dissipation hole 154 corresponding to the second fan housing 1371, the heat dissipation hole 154 communicates the atmosphere with the cavity, and the cavity may have a ventilation opening communicating with the accommodating cavity.

In order to prevent ash falling, the nucleic acid amplification detecting apparatus 10 further includes a housing 150, the housing 150 includes an upper housing wall 151 and a lower housing wall, the upper housing wall 151 is connected with the lower housing wall and forms a receiving chamber (not shown), wherein the fixing frame 100, the optical module 110, the driving mechanism 120, the temperature control mechanism 130, and the processor 160 are located in the receiving chamber.

The connection mode of upper housing wall 151 and the connection of inferior valve wall can be for riveting, welding etc. also can be for can dismantling the connection, and for the convenience of maintenance, upper housing wall 151 can be dismantled with inferior valve wall and be connected, can dismantle the mode of connecting for example for joint, bolted connection etc..

In this embodiment, referring to fig. 1 and 11, the lower casing wall further includes a first side plate 1533 connected to the base 1531, and the first side plate 1533 is located on a side of the base 1531 away from the elastic buffer layer, so that the cross section of the lower casing wall is "L" shaped. In other words, the lower casing wall is formed by the base 1531 and the first side plate 1533. At this time, the processor 160 is disposed on the first side plate 1533 and located in the accommodating cavity, so as to improve the utilization of the first side plate 1533.

Optionally, the first side plate 1533 and/or the base 1531 both have a heat dissipation area, and the heat dissipation area may also be provided with a plurality of heat dissipation holes 154 communicating with the atmosphere and the accommodating cavity, so as to dissipate heat from the hot air in the accommodating cavity, wherein the plurality of heat dissipation holes 154 may be distributed in an array. For example, the base 1531 may have a plurality of heat dissipation holes 154 corresponding to the mounting position of the power filter 140, each of the heat dissipation holes 154 is communicated with the atmosphere and the accommodating cavity, and the first side plate 1533 has a heat dissipation region.

Optionally, the processor 160 is disposed on the first side plate 1533, which is convenient for assembly and disassembly and reasonable in wiring.

Optionally, the first side plate 1533 is provided with a USB interface 155 and/or a network interface 156, and the USB interface 155 and/or the network interface 156 are arranged at intervals, in this embodiment, the first side plate 1533 is provided with the USB interface 155 and the network interface 156.

The USB interface 155 is connected to the processor 160, so that the processor 160 implements a function of importing and exporting data through the USB interface 155. The network port is connected to the processor 160, so that the processor 160 can implement a networking function through the network port via a network cable.

Since the nucleic acid amplification detecting apparatus 10 may vibrate during operation, an elastic buffer member 157, such as a plurality of rubber feet, may be disposed on a side of the base 1531 away from the receiving cavity for supporting and damping the nucleic acid amplification detecting apparatus 10.

In practical use, the microfluidic chip 20 is located in the mounting cavity, so that, in order to facilitate mounting or dismounting the microfluidic chip 20 from the mounting region 103, the first opening 105 is disposed on a side wall of the second groove 101 that is away from the first side plate 1533 along the first predetermined direction, and meanwhile, the upper housing wall 151 is provided with a second opening 158 and a hatch 159 for closing the second opening 158 at a position corresponding to the fixing frame 100.

The position of the upper housing wall 151 corresponding to the fixing frame 100 is: the projection of the holder 100 onto the upper housing wall 151 in the first predetermined direction, and optionally the projection of the movement range of the holder 100 onto the upper housing wall 151 in the second predetermined direction in the first predetermined direction, can be performed under the setting condition that the microfluidic chip 20 can be mounted no matter where the holder 100 is stopped, after the hatch 159 is opened. Optionally, the area of the mounting opening is larger than the projection of the holder 100 onto the upper housing wall 151.

Optionally, one end of the hatch 159 is hinged to the upper housing wall 151, and the other end of the hatch 159 is detachably connected to the upper housing wall 151, for example, by clamping, so as to close the hatch 159, before use, the hatch 159 is opened, the microfluidic chip 20 can be directly mounted in the mounting area 103, meanwhile, the hatch 159 is closed to perform nucleic acid amplification and detection, and after detection is completed, the hatch 159 is opened again, and the microfluidic chip 20 is taken out.

The upper housing wall 151 is provided with a through hole through which the display screen 170 is mounted on the upper housing wall 151, thereby facilitating observation of results. Meanwhile, since the display screen 170 is connected to the processor 160 through a wire, the circuit between the display screen 170 and the processor 160 is not affected after the upper housing wall 151 is separated from the processor during the maintenance process. Optionally, the display screen 170 is a touch screen, and the adjustment of the relevant parameters can be performed through touch keys and internal software thereof.

Alternatively, the base 1531 is provided with a bracket 171, the display screen 170 is fixed to the bracket 171, and specifically, for example, the bracket 171 is located in a direction away from the first side plate 1533 of the base 1531, and the display screen 170 is disposed obliquely. The display screen 170 is in close contact with the wall of the through hole, thereby effectively preventing dust from falling. Meanwhile, there is enough space between the first side plate 1533 and the bracket 171 to mount the components.

Optionally, the periphery of the display screen 170 may be clamped to the inner wall of the through hole, so as to facilitate the detachment and installation. An installation gap is formed between the bracket 171 and the base 1531, wherein all or part of the fixing frame 100 and the optical module 110 can be disposed in the installation gap, which effectively improves the space utilization and the structure compactness.

It should be noted that the upper housing wall 151 may also be provided with heat dissipation holes 154 according to the requirement, which is not described herein.

The workflow of the nucleic acid amplification detecting apparatus 10 is as follows:

during detection, the hatch 159 is opened, the microfluidic chip 20 is inserted into the fixing frame 100 and fixed to the fixing frame 100 through the positioning and fixing structure 106, the hatch 159 is closed, and the detection is started by setting the parameter equipment through the display screen 170. In the detection process, the processor 160 controls the temperature control mechanism 130 to heat, so that the sample in the microfluidic chip 20 and the pre-embedded primers in the microfluidic chip 20 generate an amplification reaction, the controller controls the excitation light source 111 to generate excitation light, the controller controls the driving mechanism 120 to drive the fixing frame 100 to move along a first preset direction and a second preset direction, so that the detection hole 104 passes through the detection light beam and is gradually excited corresponding to each reaction cell, the excitation light is transmitted into the photomultiplier through the reflector 1151, the fluorescence signal received by the photomultiplier is transmitted to the processor 160 and displayed in the display screen 170, and the internal processor 160 software generates data so as to determine the detection result.

Example 2

The present application provides a nucleic acid amplification detection system (not shown in the figures), which includes the nucleic acid amplification detection apparatus provided in example 1 and a microfluidic chip used in cooperation therewith, wherein the microfluidic chip is mounted on a fixing frame, and reaction cells of the microfluidic chip are arranged along a first preset direction and/or a second preset direction.

Please refer to embodiment 1 for the way that the microfluidic chip is mounted on the fixing frame, which is not described herein.

In summary, according to the nucleic acid amplification detection apparatus and system of the present application, the existing centrifugation method (centrifugal force is generated) is replaced by the movement method in which the fixing frame moves linearly along the first preset direction and the second preset direction, the microfluidic chip and the fixing frame are kept relatively still, and the sample in the reaction cell is kept relatively still, so that even if the reaction cells of the microfluidic chip are communicated with each other, the problem of the existing "possible cross contamination between the reaction cells" is reduced. Meanwhile, each reaction cell of the microfluidic chip can be scanned step by step, so that the data accuracy of each reaction cell can be ensured. The optical module is used for generating a detection light beam which is preset to point, namely, the optical module is in a fixed state in the whole detection process, the stability of a fluorescence signal is guaranteed, and the data accuracy of each reaction cell is further guaranteed by matching with a moving mode.

The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims (10)

1. A nucleic acid amplification detection device, suitable for use with a microfluidic chip, comprising:

the fixing frame is defined with a first preset direction and a second preset direction which are perpendicular to each other, the fixing frame is provided with a mounting area for mounting the microfluidic chip, and the mounting area is provided with a detection hole which penetrates through the fixing frame and corresponds to a reaction tank of the microfluidic chip;

the optical module is used for generating a detection light beam with a preset direction so as to detect the sample and obtain a fluorescence signal;

a base;

and the driving mechanism is fixed on the base and connected with the fixing frame and used for driving the fixing frame to move along the first preset direction and the second preset direction so that the detection hole passes through the detection light beam.

2. The nucleic acid amplification detection apparatus according to claim 1, wherein the drive mechanism comprises:

the first drive mechanism includes: the first stepping motor is in transmission connection with the first sliding block and is used for driving the first sliding block to reciprocate along a first preset direction;

a second drive mechanism comprising: the second fixed plate is fixed on the base, the second sliding block is connected with the first fixed plate, and the second stepping motor is arranged on the second fixed plate and is in transmission connection with the second sliding block and used for driving the second sliding block to reciprocate along a second preset direction.

3. The nucleic acid amplification detection apparatus according to claim 2, wherein the first drive mechanism comprises:

the limiting plate is fixed on the first fixing plate, and the first stepping motor is fixed on the limiting plate;

the motor driving plate is connected with the first stepping motor and controls the motion of the first stepping motor;

the photoelectric switch is arranged on the limiting plate and forms a photoelectric switch induction area used for obtaining optical signals, and the photoelectric switch is connected with the motor driving plate;

and the limiting piece is arranged on the first sliding block and protrudes out of the first sliding block, so that when the first sliding block is abutted against the limiting plate, the limiting piece is positioned in the photoelectric switch sensing area and shields the optical signal.

4. The nucleic acid amplification detection apparatus of claim 1, wherein the optical module comprises: an excitation light source;

an emission light path comprising: the device comprises a first condenser, an excitation optical filter, a first convex lens, a second convex lens, a dichroic mirror and a front focusing mirror which are arranged in sequence;

the first collecting mirror is used for receiving exciting light emitted by an exciting light source, collimating the exciting light and emitting the collimated exciting light to the exciting light filter, the exciting light filter filters the exciting light into quasi-monochromatic light, the quasi-monochromatic light is focused again through the first convex lens to be changed into parallel light, the parallel light passes through the second convex lens to obtain a collimated light spot, and the collimated light spot is reflected to the front focusing mirror through the dichroic mirror and focused through the front focusing mirror to form the detection light beam;

a receiving optical path;

and the detector receives the fluorescence transmitted by the receiving optical path to obtain a fluorescence signal.

5. The nucleic acid amplification detecting apparatus according to claim 4,

the receiving light path comprises a reflecting mirror, a fluorescent filter and a rear focusing mirror which are arranged in sequence;

the reflecting mirror is used for receiving fluorescence generated by the reaction tank under the excitation of the detection light beams and processed by the front focusing mirror and the dichroic mirror in sequence, and the fluorescence reflected by the reflecting mirror is filtered by the fluorescence filter and then focused on a receiving window of the detector by the rear focusing mirror.

6. The nucleic acid amplification detection apparatus according to claim 1, wherein the nucleic acid amplification detection apparatus comprises a temperature control mechanism, the temperature control mechanism has a heating surface, the temperature control mechanism is spaced from the mounting region, so that the heating surface and the mounting region together form a cavity for accommodating the microfluidic chip, and when the microfluidic chip is mounted on the mounting region, the heating surface can contact with a side of the microfluidic chip away from the reaction cell for heating.

7. The nucleic acid amplification detecting apparatus according to claim 6, wherein the temperature control mechanism includes a heating unit fixed to a fixing frame, the heating unit including:

the first heating block is provided with a heating surface, and a first groove is formed in one side, away from the heating surface, of the first heating block;

the second heating block is in heat conduction connection with the first heating block and mutually attached, so that the first groove and the first heating block form an installation cavity;

and the refrigerating piece is installed in the installation cavity, the cold end of the refrigerating piece is attached to the second heating block and is in heat conduction connection, and the hot end of the refrigerating piece is attached to the first heating block and is in heat conduction connection.

8. The nucleic acid amplification detecting apparatus according to claim 7, wherein the temperature control mechanism further comprises a temperature sensor provided partially or entirely to the first heating block;

optionally, the temperature control mechanism still includes temperature protection switch, temperature protection switch part or whole set up in first heating piece, the refrigeration piece warp temperature protection switch electricity is connected with the power.

9. The nucleic acid amplification detection apparatus according to claim 1, comprising: the display screen is connected with the processor;

the processor is connected with the optical module and used for receiving the fluorescence signal, generating a real-time fluorescence detection signal and displaying the real-time fluorescence detection signal through the display screen, and the processor is connected with the driving mechanism and used for controlling the driving mechanism to move along the first preset direction and the second preset direction.

10. A nucleic acid amplification detection system, comprising a microfluidic chip and the nucleic acid amplification detection apparatus according to any one of claims 1 to 9, wherein the microfluidic chip is mounted on the holder, and the reaction chambers of the microfluidic chip are arranged along a first predetermined direction and/or a second predetermined direction.

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