CN214408656U - Fluorescence excitation type detection system - Google Patents

Abstract

The utility model provides a fluorescence excitation formula detecting system belongs to biological detection technical field. This fluorescence excitation formula detecting system for detect biochip, including excitation light generator, the light path subassembly of locating in the frame, its characterized in that: the fluorescent detector also comprises a fixing piece for fixing the chip and a fluorescent detector corresponding to the fixing piece, wherein the fixing piece is provided with a two-dimensional position adjusting structure and the like which can enable the fixing piece to move along a first direction and a second direction. The fluorescence excitation type detection system has the advantages that: the size of facula in the light path can be adjusted to the light path subassembly of this scheme, can realize the excitation of a plurality of sites on the chip, also can adopt the fixed point to shine, and is specific as required and definite, has improved excitation efficiency, also is convenient for to the detection of fluorescence.

Description

Fluorescence excitation type detection system

Technical Field

The utility model belongs to the technical field of biological detection, especially, relate to a fluorescence excitation formula detecting system that adopts fluorescence excitation's method to detect to the component of the chip that carries biomaterial, concentration.

Background

The biochip technology is a miniature biochemical analysis system integrating discontinuous analysis processes in the life science field into the surface of a silicon chip or a glass chip according to the principle of specific interaction between molecules by a micro technology so as to realize accurate, rapid and large-information-quantity detection of cells, proteins, genes and other biological components. Biochips can be classified into gene chips, protein chips, polysaccharide chips, and neuron chips according to the difference in biomaterial immobilized on the chips.

The principle of fluorescence excitation is that a biological material labeled with specific fluorescein is reacted with a chip, components which are not complementarily combined with the biological material on the chip are washed away by bleaching, then the chip is excited by excitation light to generate fluorescence, the generated fluorescence is scanned by a scanner to measure the fluorescence intensity of each point on the chip, and various characteristics of the biological material on the chip are analyzed by the fluorescence intensity.

The existing detection device can only scan one single point due to the fixed position of the component for fixing the chip, and the operation is troublesome for realizing the whole scanning of the chip

SUMMERY OF THE UTILITY MODEL

It is an object of the present invention to provide a fluorescence excitation type detection system for solving at least a part of the above problems.

In order to achieve the above purpose, the utility model adopts the following technical proposal: the utility model discloses a fluorescence excitation formula detecting system for detect biochip, including laser generator, the light path subassembly of locating in the frame, its characterized in that: the fluorescent detector is characterized by also comprising a fixing piece for fixing the chip and a fluorescent detector corresponding to the fixing piece, wherein the fixing piece is provided with a two-dimensional position adjusting structure capable of enabling the fixing piece to move along the first direction and the second direction.

In the fluorescence excitation type detection system, the two-dimensional position adjusting structure comprises a first direction adjusting assembly and a second direction adjusting assembly, the second direction adjusting assembly is arranged on the first direction adjusting assembly, and the fixing piece is arranged on the second direction adjusting assembly; the first direction adjusting assembly comprises a first direction sliding seat arranged along a first direction, a first direction sliding block arranged along the first direction is arranged on the first direction sliding seat, and a first direction driving piece for driving the first direction sliding block to move forwards and backwards on the first direction sliding seat along the first direction is arranged between the first direction sliding seat and the first direction sliding block; the second direction adjusting assembly comprises a second direction sliding seat arranged along a second direction, the second direction sliding seat is arranged on the first direction sliding block, a second direction sliding block arranged along the second direction is arranged on the second direction sliding seat, a second direction driving piece for driving the second direction sliding block to move forwards and backwards along the second direction is arranged between the second direction sliding seat and the second direction sliding block, and the fixing piece is arranged on the second direction sliding block.

In the fluorescence excitation type detection system, the first direction sliding seat and the first direction sliding block, and the second direction sliding seat and the second direction sliding block are in sliding fit through a pair of sliding rails and sliding grooves which are correspondingly arranged.

In the fluorescence excitation type detection system, the first direction driving member comprises a first direction rotating handle and a first direction transmission component which is matched with the first direction rotating handle and runs along the first direction, and the first direction sliding block matched with the first direction transmission component is driven to move along the first direction by rotating the first direction rotating handle; the second direction driving piece comprises a second direction rotating handle and a second direction transmission part which is matched with the second direction rotating handle and runs along the second direction, and the second direction sliding block matched with the second direction transmission part is driven to move along the second direction by rotating the second direction rotating handle.

In the fluorescence excitation type detection system, the first direction transmission member and the second direction transmission member may include a gear and a rack, or a lead screw and a lead screw nut.

In the fluorescence excitation type detection system, the optical path component includes a parallel light adjusting member for converting scattered light emitted by the excitation light generator into parallel light, and a condenser fitted with the parallel light adjusting member and provided with an optical lens therein, wherein the condenser adjusts the size of a light spot irradiated on the fixed chip on the fixing member by adjusting the distance between the optical lens and the parallel light adjusting member.

In the fluorescence excitation type detection system, the light path component is arranged obliquely downwards, the excitation light generator is arranged below the parallel light adjusting piece, and the emission surface of the excitation light generator and the parallel light adjusting piece are arranged in an up-down corresponding manner.

In the fluorescence excitation type detection system, the fluorescence detector is arranged below the excitation light generator, and the excitation light generator and the two-dimensional position adjusting structure are positioned on the same horizontal plane.

In the fluorescence excitation type detection system described above, the fluorescence detector is an imaging device.

Compared with the prior art, the fluorescence excitation type detection system has the advantages that:

1. in the technical route of exciting fluorescence, fixed-point irradiation is generally adopted, and the light path component of the scheme can adjust the size of a light spot in a light path, so that excitation of a plurality of sites on a chip can be realized, fixed-point irradiation can also be adopted, the excitation efficiency is improved and the fluorescence can be conveniently detected according to the requirement;

2. the fluorescence detector adopts an imaging device, so that not only can single point be analyzed and detected, but also the fluorescence of all sites in the light spot can be imaged, analyzed and detected;

3. the light path component, the excitation light generator and the fluorescence detector are arranged from top to bottom, so that the system is compact in structure, and the requirement on installation space is reduced.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

Fig. 1 provides a schematic structural diagram of an embodiment of the present invention.

Fig. 2 provides a schematic view of the two-dimensional position adjustment structure of fig. 1 without a gantry, with the front side of the structure facing the excitation light directed toward itself.

Fig. 3 provides a schematic front view of fig. 2 when illuminated with light.

Fig. 4 provides a schematic top view of fig. 2 under light illumination.

Fig. 5 provides a schematic structural view of the two-dimensional position adjustment structure of fig. 1 and 2.

Fig. 6A provides a schematic structural diagram of an embodiment of the optical circuit assembly of the present invention.

Fig. 6B provides a schematic cross-sectional view in the direction a-a of fig. 6A.

Fig. 6C provides a schematic illustration of the exploded structure of fig. 6A.

Fig. 6D provides a schematic diagram of the structure of the optical concentrator of fig. 6A.

Fig. 6E provides a front view schematic of fig. 6D.

FIG. 6F provides a schematic cross-sectional view in the direction B-B of FIG. 6E.

Fig. 6G provides a side view schematic of fig. 6E.

Fig. 7A provides a schematic structural diagram of an embodiment of a condenser in an optical path assembly according to the present invention.

Fig. 7B provides a schematic illustration of the exploded structure of fig. 7A.

Fig. 7C provides a side view schematic of one side of fig. 7A.

Fig. 7D provides a schematic cross-sectional view of fig. 7C.

Fig. 7E provides a side view schematic of the other side of fig. 7A.

Fig. 7F provides a schematic diagram of the construction of the hold down block and filter frame of fig. 7A.

In the figure, a chip a, a frame 101, an excitation light generator 102, a fixing member 103, a first direction slide 104, a first direction slide 105, a second direction slide 106, a second direction slide 107, a first direction rotating handle 108, a second direction rotating handle 109, an off-axis parabolic mirror assembly 110, a CCD camera 111, an angle-type mounting bracket 112, a fastening screw 113, a condenser 3, an outer barrel 201, an inner barrel 202, a focusing ring 203, a focusing lens 204, a filter lens 205, a passing groove 206, a connecting member 207, a filter mounting base 208, a flange housing 209, a first limiting member 210, a first limiting groove 211, a ring-shaped blocking base 212, a flared portion 213, a connecting groove 214, a lens 301, a lens base 302, a lens barrel 303, a first connecting hole 304, a distance adjusting threaded rod, a first supporting base 306, a second connecting hole 307, a guiding column 308, a second supporting base 309, a filter 310, a filter groove 311, a filter frame 312, a lens holder 106, a focusing ring-shaped blocking base 203, a focusing ring-shaped blocking base 213, a focusing rod, a lens holder 305, a lens holder 310, a lens holder, a, Fixing screw 313, pressing block 314, elastic pressing part 3141 and pressing opening 315.

Detailed Description

The present invention will be described in further detail by way of examples with reference to the accompanying drawings, which are illustrative of the present invention and are not intended to limit the present invention.

As shown in fig. 1 to 5, the fluorescence excitation detection system for detecting a biochip includes an excitation light generator 102 and a light path assembly disposed on a frame 101, a fixing member 103 for fixing a biochip a, and a fluorescence detector corresponding to the fixing member 103, wherein the fixing member 103 is provided with a two-dimensional position adjustment structure capable of moving the fixing member in a first direction and a second direction.

It should be noted that the first direction and the second direction are two different directions, and an included angle formed between the first direction and the second direction may be smaller than 90 degrees, or larger than 90 degrees, or 90 degrees (that is, a relationship perpendicular to each other), and is specifically set according to needs, and the direction arrangement of the front surface of the two-dimensional position adjustment structure herein may also be changed according to needs, for example, the front surface of the two-dimensional position adjustment structure is set right opposite to the irradiated excitation light, or the front surface thereof may be set in parallel to the direction of the irradiated excitation light, so as to match the irradiation needs of the chip a fixed on the fixing member 103.

In addition, the fixing member 103 may have various structures, such as a groove-type structure, as shown in fig. 1, 2 and 5, and the fixing member 103 is a mounting seat protruding from the two-dimensional position adjustment structure and having a shape of a Chinese character 'hui', but may be configured in other structures as needed.

As shown in fig. 3 to 4, the working principle of the above system is as follows: the excitation light generator 102 starts to emit an excitation light source, the excitation light source irradiates the optical path component, light passing through the optical path component finally irradiates a chip a in the fixing member 103 on the two-dimensional position adjustment structure, the irradiated part of the chip a is excited to emit fluorescence, and the fluorescence is detected by the fluorescence detector.

The two-dimensional position adjustment structure may have a variety of configurations, and in one or some embodiments, as shown in fig. 1, 2, and 5, the two-dimensional position adjustment structure includes a first direction adjustment assembly and a second direction adjustment assembly, the second direction adjustment assembly is disposed on the first direction adjustment assembly, and the fixing member is disposed on the second direction adjustment assembly; the first direction adjusting component comprises a first direction sliding base 104 arranged along a first direction, a first direction sliding block 105 arranged along the first direction is arranged on the first direction sliding base 104, and a first direction driving piece for driving the first direction sliding block 105 to move forwards and backwards along the first direction on the first direction sliding base 104 is arranged between the first direction sliding base 104 and the first direction sliding block 105; the second direction adjusting assembly comprises a second direction sliding seat 106 arranged along a second direction, the second direction sliding seat 106 is arranged on the first direction sliding block 105, a second direction sliding block 107 arranged along the second direction is arranged on the second direction sliding seat 106, a second direction driving piece for driving the second direction sliding block 107 to move forwards and backwards along the second direction on the second direction sliding seat 106 is arranged between the second direction sliding seat 106 and the second direction sliding block 107, and the fixing piece is arranged on the second direction sliding block 107.

As shown in fig. 5, in order to facilitate the installation of the second direction adjustment assembly, an angle-shaped mounting frame 112 is provided on the first direction slider 105, the second direction adjustment assembly is provided on the angle-shaped mounting frame 112, and in addition, in order to prevent the second direction slider 107 from sliding off the second direction slider 106, a fastening screw 113 is provided, so that the fastening screw 113 is loosened before the position of the second direction slider 107 is adjusted, and the fastening screw 113 is tightened after the position of the second direction slider 107 is adjusted.

As shown in fig. 1, 2 and 5, in one or some embodiments, the first direction sliding base 104 and the first direction sliding block 105, and the second direction sliding base 106 and the second direction sliding block 107 are slidably engaged with each other through a pair of sliding rails and sliding grooves which are correspondingly arranged.

As shown in fig. 1, 2 and 5, in one or some embodiments, the first direction driving member includes a first direction rotating handle 108 and a first direction transmission member (not shown) cooperating therewith and operating in a first direction, and the first direction slider 105 cooperating with the first direction transmission member is moved in the first direction by rotating the first direction rotating handle 108; the second direction driving member includes a second direction rotating handle 109 and a second direction transmission member (not shown) cooperating therewith and moving in the second direction, and the second direction slider 107 cooperating with the second direction transmission member is moved in the second direction by rotating the second direction rotating handle 109.

In one or some embodiments, the first direction transfer component, the second direction transfer component, or comprise a gear and a rack, or comprise a lead screw and a lead screw nut.

It should be noted that the first direction transmission member may be directly disposed on the first direction rotating handle 108 and the first direction sliding block 105, for example, when the first direction transmission member is disposed by matching a gear and a rack, the gear may be disposed on the first direction rotating handle 108 and coaxially disposed therewith, the rack may be disposed on the bottom of the first direction sliding block 105 and disposed along the first direction, and when the first direction transmission member is disposed by matching a lead screw and a lead screw nut, an external thread may be disposed on the outer circumferential side of the first direction rotating handle 108 as the lead screw, a groove having an arc-shaped cross section is disposed on the bottom of the first direction sliding block 105 and along the first direction, and broken threads parallel to each other and not connected are disposed on the inner wall of the groove (in this case, the first direction sliding block 105 corresponds to the lead screw nut), so that the external thread on the first direction rotating handle 108 matches the broken threads, the purpose of moving the first direction slider 105 is achieved by rotating the first direction rotating handle 108, and the second direction transmitting member here may be directly provided on the second direction rotating handle 109 and the second direction slider 107, as in the first direction transmitting member described above.

In order to improve the compactness of the system, as shown in fig. 1, in one or some embodiments, the light path component is disposed obliquely downward, the excitation light generator 102 is disposed below the parallel light adjusting member, and the emission surface of the excitation light generator 102 and the parallel light adjusting member are disposed in an up-and-down correspondence.

In one or some embodiments, the fluorescence detector is disposed below the excitation light generator 102, and the excitation light generator 102 is located on the same horizontal plane as the two-dimensional position adjustment structure.

In one or some embodiments, the fluorescence detector is an imaging device.

In addition, there are a plurality of imaging devices as the fluorescence detector, and in general, a camera such as the CCD camera 111 or the CMOS camera is used, so that not only single-point fluorescence but also multiple-point fluorescence can be simultaneously captured.

In one or some embodiments, the optical path assembly includes a parallel light adjusting member for converting the scattered light emitted from the excitation light generator 102 into parallel light, and a condenser 3 having an optical lens therein and coupled thereto, wherein the condenser 3 adjusts the size of the light spot on the fixed chip on the fixing member 103 by adjusting the distance between the optical lens and the parallel light adjusting member.

The condenser 3 may have various structures, as shown in fig. 6A to 6G, a specific structure of the condenser 3 includes an outer barrel 201 detachably disposed on the frame 101 and having a hollow structure, an inner barrel 202 sleeved in the outer barrel 201 and having a hollow structure, and a focusing ring 203 screwed to an outer side of the inner barrel 202, the focusing ring 203 is rotatably disposed on the outer barrel 201, the inner barrel 202, and the focusing ring 203 are coaxially disposed, a focusing lens 204 is disposed in the inner barrel 202, and the inner barrel 202 moves along an axial direction thereof under the action of the rotating focusing ring 203 to adjust a position of the focusing lens 204 in the inner barrel 202.

It should be noted that the focusing lens 204 receives the light from the light source generator, and the adjustment of the size of the light spot emitted from the focusing lens 204 to the biochip is achieved by moving the inner barrel 202.

As a specific structure of the above-mentioned condenser 3, as shown in fig. 6C, in one or some embodiments, the inner barrel 202 or the outer barrel 201 is further provided with a filter lens 205 matching with the focusing lens 204, and when the filter lens 205 is provided on the outer barrel 201, a passing groove 206 for passing a connecting piece 207 for detachably connecting the filter lens 205 and the outer barrel 201 is provided on the outer side of the inner barrel 202 along the axial direction thereof.

As a specific structure of the above-mentioned condenser 3, in order to facilitate replacement and installation of the filter 205, as shown in fig. 6C, in one or some embodiments, when the filter 205 is located on the outer barrel 201, a filter mounting seat 208 is detachably disposed on one end of the outer barrel 201 away from the inner barrel 202, the filter 205 is disposed in the filter mounting seat 208, and one end of the connecting member 207 is connected to the filter 205 and the other end is connected to the filter mounting seat 208.

As a specific structure of the condenser 3, in order to facilitate the outer barrel 201 to be disposed on the frame 101, as shown in fig. 6A, 6C and 6D, in one or some embodiments, a flange 209 connected to the frame 101 is disposed outside the outer barrel 201.

As a specific structure of the condenser 3 described above, in one or some embodiments, at least one rotation stop assembly for preventing the rotation of the inner barrel 202 when the inner barrel 202 moves in the axial direction thereof is provided between the inner barrel 202 and the outer barrel 201.

It should be noted that, the rotation stopping assembly has various structures, and as shown in fig. 6D to 6G, in one or some embodiments, it includes a first limiting member 210 protruding from the outer side of the inner barrel 202, and a first limiting groove 211 opened on the outer barrel 201 and engaged with the first limiting member 210. Another specific structure of the rotation stopping assembly, in another embodiment or some embodiments, includes a second limiting member (not shown) protruding from the inner side of the outer barrel 201, and a second limiting groove (not shown) opening on the inner barrel 202 and engaged with the second limiting member.

It should be noted that the first limiting member 210 and the second limiting member can have various structures, as shown in fig. 6D to 6G, the first limiting member 210 is a cylindrical pin, and the first limiting groove 211 is a bar shape.

As a specific structure of the condenser 3, in order to facilitate the installation and replacement of the focusing lens 204, in one or some embodiments, the focusing lens 204 is disposed at one end of the inner barrel 202 close to the light source, and as shown in fig. 6C and 6F, a flared portion 213 is disposed at one end of the inner barrel 202 close to the light source, an internal thread is disposed in the flared portion 213, the focusing lens 204 is disposed in the flared portion 213, and the focusing lens 204 is limited by a pressing ring (not shown).

As a specific structure of the condenser 3, in order to prevent the focusing ring 203 from rotating to the other end of the inner barrel 202 and then being unscrewed from the inner barrel 202, in one or some embodiments, an annular blocking platform 212 for blocking the focusing ring 203 is convexly disposed on an outer portion of the inner barrel 202 near one end of the light source.

As shown in fig. 7A to 7F, as another specific structure of the condenser 3, it includes a lens holder 302 having a hollow structure for mounting a lens 301, and a lens barrel 303 having a hollow structure, and a distance adjusting assembly for adjusting a distance between the lens holder 302 and the lens barrel 303 is provided therebetween.

It should be noted that the structure of the distance adjusting assembly may be various, as shown in fig. 7B, 7C, 7D and 7E, and as a specific structure of the distance adjusting assembly, in one or some embodiments, the distance adjusting assembly includes a distance adjusting threaded rod 305, and first connecting through holes 304 which are matched with the distance adjusting threaded rod and arranged on the outer side of the lens holder 302 or arranged on the outer side of the lens holder 302 and the outer side of the lens barrel 303 respectively, and the inner side of at least one of the first connecting through holes 304 is provided with an internal thread matched with an external thread on the distance adjusting threaded rod 305.

As another specific structure of the condenser 3 described above, as shown in fig. 7B, in one or some embodiments, the outer side of the lens barrel 303 is provided with at least one first supporting seat 306 for supporting the distance adjusting threaded rod 305 along the axial direction thereof, and the first connecting through hole 304 on the outer side of the lens barrel 303 is opened on the first supporting seat 306.

As another specific structure of the condenser 3, in order to improve the stability of the movement of the distance adjusting assembly when adjusting the distance between the lens barrel 303 and the lens holder 302, in one or some embodiments, a guiding assembly is provided between the lens holder 302 and the lens barrel 303.

It should be noted that the structure of the guiding component can be various, as shown in fig. 7A, 7C and 7D, and as a specific structure of the guiding component, in one or some embodiments, it includes a guiding column 308 and a second connecting through hole 307 which is matched with the guiding column and is opened on the outer side of the lens holder 302 or respectively opened on the outer side of the lens holder 302 and the outer side of the lens barrel 303.

As another specific structure of the condenser 3, in order to facilitate the installation of the guide post 308, as shown in fig. 7A and 7B, in one or some embodiments, at least one second support seat 309 for supporting the guide post 308 is provided on the outer side of the lens barrel 303 along the axial direction thereof, and a second connection through hole 307 on the outer side of the lens barrel 303 is opened on the second support seat 309.

In this way, the guiding column 308 can be inserted into the second through hole 307 formed in the second supporting seat 309, and in addition, the second supporting seat 309 can be detachably connected to the lens barrel 303 through a screw, which is convenient for disassembly and assembly.

As another specific structure of the condenser 3, as shown in fig. 7A, in one or some embodiments, the lens barrel 303 is provided with a filter groove 311 for inserting the filter 310.

As another specific structure of the above-mentioned condenser 3, as shown in fig. 7A, 7B and 7F, in one or some embodiments, the filter 310 is detachably disposed in the filter frame 312, and the filter frame 312 is provided with a fixing screw 313 for fixing the filter 310 disposed therein, so that the filter 310 can be first installed outside the filter slot 311 in the filter frame 312, and then the filter frame 312 with the filter 310 installed therein is installed in the filter slot 311, and a plurality of such filter frames 312 can be further provided, each filter frame 312 is provided with a different filter 310, and each filter 310 receives radiation with different wavelengths, and can be quickly assembled into the filter slot 311 as needed, and storage of the filter 310 is also facilitated.

As another specific structure of the condenser 3, as shown in fig. 7A, 7B, and 7F, in one or some embodiments, a pressing opening 315 for a pressing block 314 to enter and exit is formed on one side of the lens barrel 303, which is located at the filter slot 311, and the pressing block 314 enters the pressing opening 315 and is then fixedly connected to the lens barrel 303 through a screw.

Note that the focusing lens 204 and the lens 301 described above have the same meaning, and the filter lens 205 and the filter 310 have the same content.

The specific embodiments described herein are merely illustrative of the spirit of the invention. Various modifications, additions and substitutions for the specific embodiments described herein may be made by those skilled in the art without departing from the spirit of the invention or exceeding the scope of the invention as defined in the accompanying claims.

Although the chip a, the frame 101, the excitation light generator 102, the fixing member 103, the first direction slider 104, the first direction slider 105, the second direction slider 106, the second direction slider 107, the first direction rotating handle 108, the second direction rotating handle 109, the off-axis parabolic mirror assembly 110, the CCD camera 111, the angle mount 112, the fastening screw 113, the condenser 3, the outer barrel 201, the inner barrel 202, the focusing ring 203, the focusing lens 204, the filter lens 205, the through groove 206, the connecting member 207, the filter mount 208, the flange housing 209, the first stopper 210, the first stopper groove 211, the ring-shaped blocking stage 212, the flared portion 213, the connecting groove 214, the lens 301, the lens holder 302, the barrel 303, the first connecting through hole 304, the pitch adjusting threaded rod 305, the first support base 306, the second connecting through hole 307, the guide post 308, the second support base 309, the filter 310, the filter groove 311, the lens barrel 311, the first coupling groove 201, the second coupling groove 201, the inner barrel 202, the focusing ring-shaped blocking stage 203, the focusing lens 204, the first coupling hole 304, the pitch adjusting threaded rod 305, the second coupling through hole 307, the guide post 308, the second support rod 309, and the filter 310, The terms filter frame 312, set screw 313, compression block 314, resilient compression portion 3141, compression port 315, etc., do not exclude the possibility of using other terms. These terms are used merely to more conveniently describe and explain the nature of the present invention; they are to be construed in a manner that is inconsistent with the spirit of the invention.

Claims (9)

1. The utility model provides a fluorescence excitation formula detecting system for detect biochip, including locating excitation light generator (102), the light path subassembly on frame (101), its characterized in that: the fluorescent detector is characterized by further comprising a fixing piece (103) used for fixing the chip and a fluorescent detector corresponding to the fixing piece, wherein the fixing piece (103) is provided with a two-dimensional position adjusting structure capable of enabling the fixing piece to move along a first direction and a second direction.

2. The fluorescence excitation-type detection system according to claim 1, wherein the two-dimensional position adjustment structure comprises a first direction adjustment assembly and a second direction adjustment assembly, the second direction adjustment assembly is disposed on the first direction adjustment assembly, and the fixing member is disposed on the second direction adjustment assembly; the first direction adjusting assembly comprises a first direction sliding base (104) arranged along a first direction, a first direction sliding block (105) arranged along the first direction is arranged on the first direction sliding base (104), and a first direction driving piece for driving the first direction sliding block (105) to move forwards and backwards along the first direction on the first direction sliding base (104) is arranged between the first direction sliding base (104) and the first direction sliding block (105); the second direction adjusting assembly comprises a second direction sliding seat (106) arranged along a second direction, the second direction sliding seat (106) is arranged on a first direction sliding block (105), a second direction sliding block (107) arranged along the second direction is arranged on the second direction sliding seat (106), a second direction driving piece for driving the second direction sliding block (107) to move forwards and backwards along the second direction on the second direction sliding seat (106) is arranged between the second direction sliding seat (106) and the second direction sliding block (107), and the fixing piece is arranged on the second direction sliding block (107).

3. The fluorescence excitation-type detection system according to claim 2, wherein the first direction slide (104) and the first direction slide (105) and the second direction slide (106) and the second direction slide (107) are slidably engaged with each other through a pair of sliding rails and sliding grooves which are correspondingly arranged.

4. The fluorescence excitation-type detection system according to claim 2, wherein the first direction driving member comprises a first direction rotating handle (108) and a first direction transmission member cooperating therewith and operating in a first direction, and the first direction slider (105) cooperating with the first direction transmission member is moved in the first direction by rotating the first direction rotating handle (108); the second direction driving piece comprises a second direction rotating handle (109) and a second direction transmission part which is matched with the second direction rotating handle and runs along the second direction, and a second direction sliding block (107) matched with the second direction transmission part is driven to move along the second direction by rotating the second direction rotating handle (109).

5. The fluorescence excitation-type detection system according to claim 4, wherein the first direction transmission member, the second direction transmission member, or the gear and the rack, or the screw and the screw nut.

6. The fluorescence excitation type detection system according to claim 1, wherein the optical path assembly comprises a parallel light adjusting member for converting the scattered light emitted from the excitation light generator (102) into parallel light, and a condenser (3) provided with an optical lens therein and engaged therewith, wherein the condenser (3) adjusts the size of the light spot on the fixed chip on the fixing member (103) by adjusting the distance between the optical lens and the parallel light adjusting member.

7. The fluorescence excitation detection system according to claim 6, wherein the optical path assembly is disposed obliquely downward, the excitation light generator (102) is disposed below the parallel light adjuster, and an emission surface of the excitation light generator (102) and the parallel light adjuster are disposed in an up-down correspondence.

8. The fluorescence excitation detection system according to claim 1, wherein the fluorescence detector is disposed below the excitation light generator (102), and the excitation light generator (102) and the two-dimensional position adjustment structure are located on the same horizontal plane.

9. The fluorescence excitation-type detection system according to claim 1, wherein the fluorescence detector is an imaging device.

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