CN108181290B - Fluorescent quantitative analyzer - Google Patents
Fluorescent quantitative analyzer Download PDFInfo
- Publication number
- CN108181290B CN108181290B CN201810248662.9A CN201810248662A CN108181290B CN 108181290 B CN108181290 B CN 108181290B CN 201810248662 A CN201810248662 A CN 201810248662A CN 108181290 B CN108181290 B CN 108181290B
- Authority
- CN
- China
- Prior art keywords
- optical fiber
- light source
- detection
- collecting
- parallel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Optics & Photonics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention provides a fluorescence quantitative analyzer, which relates to the technical field of medical detection instruments and comprises a machine body, a thermal printer arranged at the top of the machine body and a fishtail handle arranged at the bottom of the machine body, wherein the machine body is of a hollow cuboid structure, a liquid crystal screen for displaying data is arranged on the front surface of the machine body, and a detection inlet for placing an object to be detected is arranged on the side surface of the machine body; the front of the fishtail handle is provided with a keyboard for information interaction, and the back of the machine body is provided with a battery box for placing batteries. The fluorescence quantitative analyzer provided by the invention has a compact structure, is portable and easy to use, analyzes and detects human blood and urine based on the technical principle of fluorescence immunity and immunochromatography, and solves the technical problems of complex mechanical transmission structure and low utilization rate of light path devices of the existing analyzer.
Description
Technical Field
The invention relates to the technical field of medical detection instruments, in particular to a fluorescence quantitative analyzer.
Background
Fluorescence Immunoassay (FIA) is a microanalysis method developed in recent years, and is one of the most sensitive microanalysis techniques at present. The method of qualitatively or quantitatively analyzing a substance based on the spectrum and intensity of fluorescence, which is emitted by absorbing energy by molecules of the substance, is called fluorescence analysis. The Fluorescence Immunoassay (FIA) has the advantages of high sensitivity, strong selectivity, small sample quantity, simple method and the like, the lower limit of the method is 2-4 orders of magnitude higher than that of the spectrophotometry, and the method is widely applied to biochemical analysis.
The conventional universal fluorescent quantitative detector is usually large in size and inconvenient to use in a portable way, so that the development of the fluorescent quantitative detector in underdeveloped areas with inconvenient traffic is limited. Even some commercial products have the defects of complex structure, poor reliability, low precision, high operation difficulty and the like. Therefore, achieving instrument portability and scalability is a problem that needs to be addressed in the art.
Disclosure of Invention
Aiming at the problems, the invention aims to provide a fluorescent quantitative analyzer which is compact in structure, portable and easy to use, analyzes and detects human blood and urine based on the technical principle of fluorescence immunity and immunochromatography, and solves the technical problems of complex mechanical transmission structure and low utilization rate of light path devices of the existing analyzer.
The invention provides a fluorescence quantitative analyzer, which is characterized by comprising a machine body, a thermal printer arranged at the top of the machine body and a fishtail handle arranged at the bottom of the machine body, wherein: the machine body is of a hollow cuboid structure, a liquid crystal screen for displaying data is arranged on the front face of the machine body, and a detection inlet for placing an object to be detected is formed in the side face of the machine body; the front side of the fishtail handle is provided with a keyboard for information interaction, and the back side of the machine body is provided with a battery box for placing batteries;
still including being used for inserting the detection card that detects the entry, the detection card includes the pick-up plate, lays the test paper strip on the pick-up plate and the application of sample pad that sets up between pick-up plate and the test paper strip, wherein: the front surface of the detection plate is downwards sunken to form a concave table for containing the test strip, the back surface of the detection plate is provided with a transmission rack, one end of the detection plate is erected and then extends inwards to form a suspended table, the other end of the detection plate is erected to form a support plate with the same height as the suspended table, the suspended table is positioned at the top of the sample adding pad, and a sample adding hole is formed in the suspended table;
a detection channel communicated with the detection inlet is arranged in the machine body, an observation window is arranged at the top of the detection channel, and a transmission notch is arranged at the bottom of the detection channel;
the machine body is internally provided with a scanning transmission module for driving the detection card to move, the scanning transmission module comprises a transmission gear, a stepping motor for driving the transmission gear to rotate and a microswitch for sensing the position of the detection card, the transmission gear is arranged in the transmission notch and drives the detection card to move repeatedly by meshing a transmission rack, and the microswitch is arranged at the bottom of the detection channel;
the first light source channel is parallel to the detection channel and comprises a light source and a light source optical filter, wherein the light source optical filter is coaxially arranged on one side of the light source;
the inside first reflection passageway that is used for receiving feedback fluorescence that still is provided with of fuselage, first reflection passageway perpendicular to detection channel just includes coaxial first plano-convex lens, detection light filter, second plano-convex lens, grating and the first fluorescence sensor that sets up, is provided with slope 45 at the crossing department of the axis of first reflection passageway and first light source passageway and is used for the dichroic mirror of split, wherein: the first plano-convex lens is installed at the top of the observation window, the dichroic mirror is located between the first plano-convex lens and the detection optical filter and is located on the same side of the light source as the light source optical filter, and the first fluorescence sensor is used for measuring and receiving the numerical value of the feedback fluorescence.
Further, the fuselage is inside still to be provided with and to collect optic fibre, second fluorescence sensor and two sets of coupling components, the position that the fuselage side corresponds to collecting optic fibre still is provided with the fiber interface, wherein: the collecting optical fiber comprises an incident optical fiber for collecting light into the light source, a receiving optical fiber for leading out fluorescence, and a collecting end for collecting the incident optical fiber and the receiving optical fiber; a coupling component is arranged between the incident optical fiber and the light source, and a coupling component is also arranged between the receiving optical fiber and the second fluorescence sensor; the aggregation end is mounted within the fiber optic interface.
Further, the collection fibers comprise parallel collection fibers or perpendicular collection fibers, wherein:
the parallel collecting optical fiber comprises parallel incident optical fibers, parallel receiving optical fibers and parallel collecting ends, wherein the parallel incident optical fibers are parallel to each other and used for collecting a light source, the parallel receiving optical fibers are used for leading out fluorescence, and the parallel collecting ends are arranged in the optical fiber interfaces and used for collecting the parallel incident optical fibers and the parallel receiving optical fibers;
the vertical collecting optical fiber comprises a vertical incidence optical fiber and a vertical receiving optical fiber which are parallel to each other, and a vertical collecting end which is perpendicular to the vertical incidence optical fiber and the vertical receiving optical fiber respectively, the vertical incidence optical fiber is used for collecting a light source, the vertical receiving optical fiber is used for leading out fluorescence, and the vertical collecting end is arranged in the optical fiber interface and is used for collecting the vertical incidence optical fiber and the vertical receiving optical fiber.
Furthermore, the parallel collecting end is composed of a parallel incidence optical fiber positioned in the center and a parallel receiving optical fiber uniformly surrounding the parallel incidence optical fiber.
Furthermore, the vertical collecting end is composed of a vertical incidence optical fiber positioned in the center and a vertical receiving optical fiber uniformly surrounding the outer side of the vertical incidence optical fiber.
The optical fiber probe further comprises an optical fiber bundle detachably connected with the optical fiber interface, one end of the optical fiber bundle is connected with the collecting end of the collecting optical fiber through a connector, and the other end of the optical fiber bundle is connected with the optical fiber probe through the connector.
Further, the coupling assembly comprises a first convex lens, a narrow band filter and a second convex lens which are coaxially arranged.
Furthermore, the test strip is provided with a detection band for capturing the antigen-antibody complex, a control band for enriching the free fluorescent marker and a two-dimensional code for recording the number.
Further, still be provided with the circuit board in the fuselage, the circuit board include main control chip, amplify filter circuit, fluorescence sensor, light source drive circuit and with ID chip, power supply circuit, AD converting circuit, chronogenesis drive circuit, synchronous control circuit and the motor drive circuit that main control chip links to each other respectively, wherein:
the main control chip is respectively connected with the liquid crystal screen, the keyboard and the thermal printer;
the main control chip acquires the information of the fluorescence sensor through the A/D conversion circuit and the amplifying and filtering circuit in sequence;
the fluorescence sensor is connected with the time sequence driving circuit;
the main control chip drives the light source to work through the synchronous control circuit and the light source driving circuit in sequence;
the main control chip drives the stepping motor to control through a click driving circuit;
the main control chip is connected with the positive electrode and the negative electrode in the battery box through a power circuit.
Further, the light source comprises a laser light source and a hernia lamp light source.
The fluorescent quantitative analyzer has the following beneficial effects:
1) the fluorescent quantitative analyzer is compact in structure, a transmission scheme of matching a rack on the back of the detection card with a gear of a rotating shaft of the stepping motor is adopted, a transmission motion platform scheme is replaced, and devices such as a sliding block, a ruler belt, a guide rail, a test strip slot plate and a positioning device are reduced. Meanwhile, by designing the shape of the detection plate, the detection card can be guided to move by utilizing the shape of the detection channel on the one hand, and a user can conveniently drip samples on the other hand.
2) The fluorescence quantitative analyzer is portable and easy to use, comprises a thermal printer, a battery box and a liquid crystal screen, and can be continuously used under the condition of no external power supply.
3) The fluorescence quantitative analyzer has strong expandability and is provided with an external optical fiber interface, and a reaction area outside the analyzer can be directly analyzed through an optical fiber bundle, a connector and an optical fiber probe. Meanwhile, the light path is reasonably designed, and the light source is shared.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic front view of a first embodiment of the present invention;
FIG. 2 is a schematic view of a backside structure of the first embodiment of the present invention;
FIG. 3 is a schematic side view of the first embodiment of the present invention;
FIG. 4 is a schematic diagram of an internal structure of a detection card according to a first embodiment of the present invention;
FIG. 5 is a front view of a detection card according to a first embodiment of the present invention;
FIG. 6 is a schematic view of a back structure of a detection card according to a first embodiment of the present invention;
fig. 7 is a schematic view of the internal structure of the fuselage according to the first embodiment of the invention;
FIG. 8 is a schematic diagram of the operation of the detection card according to the first embodiment of the present invention;
FIG. 9 is a schematic circuit diagram illustrating a first embodiment of the present invention;
FIG. 10 is a schematic connection diagram of a light source driving circuit according to a first embodiment of the present invention;
FIG. 11 is a schematic diagram of the connection of the amplifying and filtering circuit according to the first embodiment of the present invention;
FIG. 12 is a schematic diagram of the connection of the A/D conversion circuit according to the first embodiment of the present invention;
FIG. 13 is a schematic side view of a second embodiment of the present invention;
FIG. 14 is a schematic front view of a second embodiment of the present invention;
fig. 15 is a schematic view of the internal structure of the fuselage of the second embodiment of the invention;
FIG. 16 is a structural diagram of a coupling assembly according to a second embodiment of the present invention;
FIG. 17 is a schematic diagram of a parallel collection optical fiber configuration according to a second embodiment of the present invention;
FIG. 18 is a schematic view of a second embodiment of the optical fiber bundle of the present invention;
fig. 19 is a schematic structural diagram of an optical fiber connector according to a third embodiment of the invention.
Fig. 20 is a schematic view of the internal structure of the fuselage of the third embodiment of the invention;
FIG. 21 is a schematic view of a vertical collection optical fiber according to a third embodiment of the present invention;
FIG. 22 is a schematic view of a third embodiment of the fiber bundle configuration of the present invention;
in the figure: 1-machine body, 101-liquid crystal screen, 102-detection inlet, 103-optical fiber interface, 2-fishtail handle, 201-keyboard, 202-battery box, 3-thermal printer, 4-detection card, 401-detection board, 402-test paper strip, 403-sample adding pad, 4011-concave table, 4012-suspension table, 4013-support plate, 4014-sample adding hole, 4015-rack, 4021-detection band, 4022-control band, 4023-bar code, 5-detection channel, 501-observation window, 502-transmission notch, 6-scanning transmission module, 601-transmission gear, 602-stepping motor, 603-micro switch 7-first light source channel, 701-light source, 702-light source optical filter, 8-first reflection channel, 801-first plano-convex lens, 802-dichroic mirror, 803-detection optical filter, 804-second plano-convex lens, 805-grating, 806-first fluorescence sensor, 9-circuit board, 10-coupling component, 1001-first convex lens, 1002-narrow band optical filter, 1003-second convex lens, 11-second fluorescence sensor, 12-parallel collection optical fiber, 1201-parallel incidence optical fiber, 1202-parallel receiving optical fiber, 1203-parallel collection end, 13-vertical collection optical fiber, 1301-vertical incidence optical fiber, 1302-vertical receiving optical fiber, 1303-vertical collection end, 14-optical fiber bundle, 15-connector and 16-optical fiber probe.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example one
As shown in fig. 1, a fluorescence quantitative analyzer comprises a body 1, a thermal printer 3 installed on the top of the body 1, and a fishtail handle 2 installed on the bottom of the body 1, wherein: the device comprises a machine body 1, a liquid crystal display screen 101, a detection inlet 102 and a detection control unit, wherein the machine body 1 is of a hollow cuboid structure, the front surface of the machine body 1 is provided with the liquid crystal display screen 101 for displaying data, and the side surface of the machine body 1 is provided with the detection inlet 102 for placing an object to be detected; the front of the fishtail handle 2 is provided with a keyboard 201 for information interaction, the back of the body 1 is provided with a battery box 202 for placing batteries, and the position of the battery box 202 is shown in fig. 2. Specifically, the thermal printer 3 may print the detection data.
As shown in fig. 3 and 4, the test strip further comprises a test card 4 for inserting into the test inlet 102, wherein the test card 4 comprises a test board 401, a test strip 402 mounted on the test board 401, and an application pad 403 arranged between the test board 401 and the test strip 402, wherein: the front surface of the detection plate 401 is recessed downwards to form a concave table 4011 for containing the test strip 402, as shown in fig. 5; the back of the detection plate 401 is provided with a transmission rack 4015, as shown in fig. 6; one end of the detection plate 401 extends inwards to form a suspension table 4012 after being erected, the other end of the detection plate 401 is erected to form a support plate 4013 with the same height as the suspension table 4012, the suspension table 4012 is located at the top of the sample adding pad 403, and a sample adding hole 4014 is formed in the suspension table 4012.
Specifically, the heights of the suspension table 4012 and the supporting plate 4013 should be kept consistent, and should be slightly lower than the height in the detection channel, so as to ensure that the detection card 4 can move smoothly in the detection channel and does not shake.
Specifically, the test strip 402 is provided with a detection strip 4021 for capturing an antigen-antibody complex, a control strip 4022 for enriching a free fluorescent marker, and a two-dimensional code 4023 for recording a number.
Example of the test procedure: first, a detection buffer (CRP antibody 1 labeled with a fluorescent marker) and a blood sample are mixed, and the CRP antibody 1 in the detection buffer and CRP (antigen) in blood react to form an antigen-antibody complex. When the mixture sample passes through the sample adding hole 4014 and the test strip 402 is immersed in the test strip 402, the mixture sample rapidly flows on the test strip 402 through capillary action, the CRP antigen-antibody complex is captured by the CRP antibody 2 immobilized on the detection zone 4021, and is enriched or trapped on the detection zone 4021 of the test strip 402, and the free fluorescent marker is enriched at the control zone 4022 by crossing the detection zone 4021. The more CRP in the blood sample, the more the complex on the detection zone 4021 accumulates, and the content of the fluorescent substance on the detection zone 4021 has a certain correspondence with the CRP concentration.
As shown in fig. 7, a detection channel 5 communicated with the detection inlet 102 is arranged inside the machine body 1, an observation window 501 is arranged at the top of the detection channel 5, and a transmission notch 502 is arranged at the bottom of the detection channel 5; the inside scanning drive module 6 that drives and detect 4 removals of card of fuselage 1 still is provided with, scanning drive module 6 includes drive gear 601, drives drive gear 601 rotatory step motor 602 and the micro-gap switch 603 of the 4 positions of response detection card, drive gear 601 sets up drive detection card 4 repetitive motion through meshing transmission rack 4015 in the transmission breach 502, micro-gap switch 603 sets up the bottom at detection passageway 5.
Specifically, the outer contour of the detection card 4 is slightly smaller than the outer contour of the detection channel 5. When a user puts a detection card 4 to be detected into the detection channel 5, a rack 4015 at the bottom of the detection card 4 is meshed with a transmission gear 601 at the bottom of the detection channel 5, when the detection card 4 is completely put into the detection channel 5, the detection card 4 presses down a microswitch 603 switch at the bottom of the detection channel 5, at the moment, the microswitch 603 switch feeds back trigger information to a main control chip, and after the main control chip acquires information that the detection card 4 is completely inserted, the main control chip starts to control the stepping motor 602 to rotate forwards and backwards so as to realize repeated scanning of the detection card 4.
Specifically, the stepping motor 602 is a 6.5MM two-phase four-wire type stepping motor 602. Preferably, in order to reduce the friction resistance between the detection card 4 and the bottom of the detection channel 5, a ball or roller may be disposed at the bottom of the inner wall of the detection channel 5, so that the sliding friction is changed into rolling friction, or a material with a low friction coefficient may be used to manufacture the detection card 4 and the detection channel 5, such as a nylon material.
As shown in fig. 7, a first light source channel 7 for generating a light source is further disposed inside the body 1, and the first light source channel 7 is parallel to the detection channel 5 and includes a light source 701 coaxially disposed and a light source filter 702 on one side of the light source 701; a first reflection channel 8 for receiving feedback fluorescence is further arranged inside the body 1, the first reflection channel 8 is perpendicular to the detection channel 5 and includes a first plano-convex lens 801, a detection filter 803, a second plano-convex lens 804, a grating 805 and a first fluorescence sensor 806, which are coaxially arranged, a dichroic mirror 802 for splitting light is arranged at the intersection of the first reflection channel 8 and the axis of the channel 7 of the first light source 701, and an angle of 45 degrees is set, wherein: the first plano-convex lens 801 is installed on the top of the observation window 501, the dichroic mirror is located between the first plano-convex lens 801 and the detection filter 803, and is located on the same side of the light source 701 as the light source filter 702, and the first fluorescence sensor 806 is used for measuring the value of the received feedback fluorescence.
Specifically, the grating 805 is a pinhole grating 805.
As shown in fig. 8, the operation of the first light source 701 channel 7 and the first reflection channel 8 is as follows: during detection, the light source 701 is turned on, excitation light passes through the collimating filter, then passes through the dichroic mirror for light splitting arranged at 45 degrees, and is focused on the surface of the test strip 402 by the focusing/collimating first plano-convex lens 801. At this time, the stepping motor 602 drives the test strip 402 to move smoothly.
The excitation light scans the detection area on the test strip 402. The fluorescence excited by the fluorescent material in the detection area on the test strip 402 is collimated into parallel light by the first plano-convex lens 801, the stray light is filtered by the dichroic mirror and the detection filter 803, and then the parallel light is focused on the confocal aperture stop by the second plano-convex lens 804. The light passing through the diaphragm is detected by the first fluorescence sensor 806 and converted into an electrical signal which, after processing and analytical calculation, is converted into a concentration of the sample being measured.
As shown in fig. 9, a circuit board 9 is further disposed in the machine body 1, the circuit board 9 includes a main control chip, an amplifying and filtering circuit, a fluorescence sensor, a light source 701 driving circuit, and an ID chip, a power circuit, an a/D conversion circuit, a timing driving circuit, a synchronous control circuit, and a motor driving circuit, which are respectively connected to the main control chip, wherein: the main control chip is respectively connected with the liquid crystal screen 101, the keyboard 201 and the thermal printer 3; the main control chip acquires the information of the fluorescence sensor through the A/D conversion circuit and the amplifying and filtering circuit in sequence; the fluorescence sensor is connected with the time sequence driving circuit; the main control chip drives the light source 701 to work through the synchronous control circuit and the light source 701 driving circuit in sequence; the main control chip drives the stepping motor 602 to control through a click driving circuit; the main control chip is connected with the positive electrode and the negative electrode in the battery box 202 through a power circuit.
In this embodiment, the light source 701 may be a hernia light source 701.
Fig. 10 is a schematic diagram of the connection of the driving circuit 701 of the light source according to the first embodiment of the present invention, in which the energy storage capacitor C is charged by the power supply, and when the voltage across the energy storage capacitor C reaches a preset value, the voltage stabilizing control element starts to operate to keep the voltage constant. When the flash lamp needs to be flashed, the main control chip controls the trigger circuit through the interface circuit, the capacitor E is rapidly discharged through the resistor Rl to generate strong current, and a ten-thousand-volt high-voltage electric pulse is generated at the secondary stage of the capacitor E through the trigger pulse transformer to ionize gas in the xenon lamp, so that the internal resistance of the lamp is greatly reduced, and a large amount of electric energy stored in the energy storage capacitor C is discharged through the pulse xenon lamp in a very short time, so that very strong flash is generated.
In the embodiment, the CA3450 operational amplifier is used for inverse amplification, and although the CA3450 completes the inverse amplification of the signal, the noise in the output signal is large, and a filter circuit is required to filter the noise, so that the signal quality is improved. Because the frequency of the signal to be obtained is relatively low, a common voltage-controlled voltage source second-order low-pass filter is selected to filter noise, as shown in fig. 11.
The sample and hold stage of this embodiment is mainly implemented by a sample/hold unit, and the quantization and coding stage is mainly implemented by an a/D converter, which uses MAX120 for the a/D converter of this embodiment. Gain adjustment and bipolar bias adjustment are realized by the potentiometers RP2 and RP1 in fig. 12, and the bias adjustment should precede the gain adjustment in the adjustment. There may be some interaction between these two adjustments, requiring repeated adjustments. The adjustment of the offset and gain is a subdivision of the a/D conversion with the aim of improving the accuracy of the a/D.
The fluorescence sensor of the present embodiment may employ a TCD1200D type CCD, or a silicon photocell.
The fluorescence quantitative analyzer provided by the embodiment has the advantages of compact structure, portability and easy use, analyzes and detects human blood and urine based on the technical principle of fluorescence immunity and immunochromatography, and solves the technical problems of complex mechanical transmission structure and low utilization rate of light path devices of the existing analyzer.
Example two
As shown in fig. 13 and 14, in this embodiment, on the basis of the first embodiment, the externally-connectable detection optical fiber is extended, and the optical fiber can be directly used for measurement of an external reaction cell of an analyzer, and is suitable for pesticide detection and seaweed detection. Specifically, the side of the body 1 is provided with an optical fiber interface 103 at a position corresponding to the collection optical fiber, the embodiment further includes an optical fiber bundle 14 detachably connected to the optical fiber interface 103, one end of the optical fiber bundle 14 is connected to the collection end of the collection optical fiber through a connector 15, and the other end of the optical fiber bundle 14 is connected to the optical fiber probe 16 through the connector 15. Specifically, the connector 15 may connect a multi-mode plastic fiber bundle, a single-mode fiber and a fiber probe, thereby generating a transmission path of the fluorescence signal and the excitation light. Specifically, the optical fiber bundle 14 may be an armored small-sized flexible optical cable, as the case may be.
As shown in fig. 15, the system further includes a parallel collection optical fiber 12, a second fluorescence sensor 11, and two sets of coupling assemblies 10, wherein: the parallel collection optical fiber 12 comprises a parallel incidence optical fiber 1201 for collecting light into the light source 701, a parallel receiving optical fiber 1202 for deriving fluorescence, and a parallel collection end 1203 for collecting the parallel incidence optical fiber 1201 and the parallel receiving optical fiber 1202; a coupling component 10 is arranged between the parallel incidence optical fiber 1201 and the light source 701, and a coupling component 10 is also arranged between the parallel receiving optical fiber 1202 and the second fluorescence sensor 11; the parallel aggregation end 1203 is mounted within the fiber optic interface 103.
As shown in fig. 16, the coupling assembly 10 includes a first convex lens 1001, a narrow-band filter 1002, and a second convex lens 1003 coaxially disposed, and the coupling lens is used to collimate the excitation light beam to improve the injection efficiency of the probe; on the other hand, the fluorescence signals can be collected to improve the utilization rate of the fluorescence signals.
The parallel collection optical fiber 12 is shown in fig. 17, the parallel collection end 1203 is shown in fig. 18, and the emergent light spot of the parallel incidence optical fiber 1201 is circularly symmetric, so that the obtained fluorescent light spot is also circularly symmetric. Therefore, the reflected light intensity is received by the symmetrically distributed optical fiber bundle 14 to improve the efficiency and sensitivity of receiving light, and in order to reduce the loss during transmission, the optical fiber used is short, about 60-80 cm.
As shown in fig. 19, the simplest method for coupling the light source 701 to the optical fiber is direct coupling, i.e., aligning the flat end surface of the optical fiber directly with the light emitting surface of the light source 701. This method is simple and convenient, but the coupling efficiency is low. If the numerical aperture of the fiber is 0.26, the maximum efficiency of direct coupling is only 6.7%. By placing a converging lens between the light source 701 and the end face of the optical fiber, the light emitting direction of the pulse xenon lamp or the receiving angle of the optical fiber is changed, and the coupling efficiency can be improved. Considering the detection environment comprehensively, the present embodiment finally selects an all-fiber structure.
The embodiment has an external optical fiber interface 103, and the reaction area outside the analyzer can be directly analyzed through the optical fiber bundle 14, the connector 15 and the optical fiber probe 16, so that the method is particularly suitable for rapid detection under ocean conditions. Meanwhile, the light path is reasonably designed, and the light source 701 is shared.
EXAMPLE III
Compared with the second embodiment, the collecting optical fiber structure of the third embodiment is different, as shown in fig. 20, the third embodiment includes a vertical collecting optical fiber 13, a second fluorescence sensor 11, and two sets of coupling assemblies 10, and an optical fiber interface 103 is further disposed on a side surface of the body 1 corresponding to a position of the collecting optical fiber, where as shown in fig. 21, the vertical collecting optical fiber 13 includes a vertical incident optical fiber 1301 and a vertical receiving optical fiber 1302 that are parallel to each other, and vertical collecting ends perpendicular to the vertical incident optical fiber 1301 and the vertical receiving optical fiber 1302, respectively, the vertical incident optical fiber 1301 is used for collecting into the light source 701, the vertical receiving optical fiber 1302 is used for leading out fluorescence, and the vertical collecting end 1303 is installed in the optical fiber interface 103 and is used for collecting the vertical incident optical fiber 1301 and the vertical receiving optical fiber 1302. As shown in fig. 22, the vertical summing end 1303 is composed of a vertically incident optical fiber 1301 at the center and vertically receiving optical fibers 1302 uniformly surrounding the outside of the vertically incident optical fiber 1301.
Specifically, since the emergent light spot of the parallel incidence optical fiber 1201 is circularly symmetric, the obtained fluorescent light spot is also circularly symmetric. Therefore, the reflected light intensity is received by the symmetrically distributed optical fiber bundle 14 to improve the efficiency and sensitivity of receiving light, and in order to reduce the loss during transmission, the optical fiber used is short, about 60-80 cm.
The C-reactive protein is detected by using the embodiment, the same fluorescent reagent with one concentration level is selected every 3 mg/L within the reagent concentration range of 3.00-39.00 mg/L for test analysis, the average value is taken for 3 times of each concentration level test to obtain the final result, the measured result is subjected to linear regression analysis, the obtained linear regression equation is that y is 0.872x +0.3, and the correlation coefficient R is20.998, the above results indicate that the instrument is a diagnostic instrumentThe linear response characteristic is good within the concentration range of 3.00-39.00 mg/L.
The embodiment has an external optical fiber interface 103, and the reaction area outside the analyzer can be directly analyzed through the optical fiber bundle 14, the connector 15 and the optical fiber probe 16, so that the method is particularly suitable for rapid detection under ocean conditions. Meanwhile, the light path is reasonably designed, and the light source 701 is shared.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (8)
1. The utility model provides a fluorescence quantitative analysis appearance which characterized in that, includes the fish tail handle of the thermal printer of fuselage, fuselage top installation and fuselage bottom installation, wherein: the machine body is of a hollow cuboid structure, a liquid crystal screen for displaying data is arranged on the front face of the machine body, and a detection inlet for placing an object to be detected is formed in the side face of the machine body; the front side of the fishtail handle is provided with a keyboard for information interaction, and the back side of the machine body is provided with a battery box for placing batteries;
still including being used for inserting the detection card that detects the entry, the detection card includes the pick-up plate, lays the test paper strip on the pick-up plate and the application of sample pad that sets up between pick-up plate and the test paper strip, wherein: the front surface of the detection plate is downwards sunken to form a concave table for containing the test strip, the back surface of the detection plate is provided with a transmission rack, one end of the detection plate is erected and then extends inwards to form a suspended table, the other end of the detection plate is erected to form a support plate with the same height as the suspended table, the suspended table is positioned at the top of the sample adding pad, and a sample adding hole is formed in the suspended table;
a detection channel communicated with the detection inlet is arranged in the machine body, an observation window is arranged at the top of the detection channel, and a transmission notch is arranged at the bottom of the detection channel;
the machine body is internally provided with a scanning transmission module for driving the detection card to move, the scanning transmission module comprises a transmission gear, a stepping motor for driving the transmission gear to rotate and a microswitch for sensing the position of the detection card, the transmission gear is arranged in the transmission notch and drives the detection card to move repeatedly by meshing a transmission rack, and the microswitch is arranged at the bottom of the detection channel;
the first light source channel is parallel to the detection channel and comprises a light source and a light source optical filter, wherein the light source optical filter is coaxially arranged on one side of the light source;
the inside first reflection passageway that is used for receiving feedback fluorescence that still is provided with of fuselage, first reflection passageway perpendicular to detection channel just includes coaxial first plano-convex lens, detection light filter, second plano-convex lens, grating and the first fluorescence sensor that sets up, is provided with slope 45 at the crossing department of the axis of first reflection passageway and first light source passageway and is used for the dichroic mirror of split, wherein: the first plano-convex lens is arranged at the top of the observation window, the dichroic mirror is positioned between the first plano-convex lens and the detection optical filter and is positioned at the same side of the light source as the light source optical filter, and the first fluorescence sensor is used for measuring the numerical value of the received feedback fluorescence;
the inside optic fibre, second fluorescence sensor and two sets of coupling subassemblies that still collect that still is provided with of fuselage, the position that the fuselage side corresponds to collecting the optic fibre still is provided with the fiber interface, wherein: the collecting optical fiber comprises an incident optical fiber for collecting light into the light source, a receiving optical fiber for leading out fluorescence, and a collecting end for collecting the incident optical fiber and the receiving optical fiber; a coupling component is arranged between the incident optical fiber and the light source, and a coupling component is also arranged between the receiving optical fiber and the second fluorescence sensor; the collecting end is arranged in the optical fiber interface;
the collection fibers comprise parallel collection fibers or perpendicular collection fibers, wherein:
the parallel collecting optical fiber comprises parallel incident optical fibers, parallel receiving optical fibers and parallel collecting ends, wherein the parallel incident optical fibers are parallel to each other and used for collecting a light source, the parallel receiving optical fibers are used for leading out fluorescence, and the parallel collecting ends are arranged in the optical fiber interfaces and used for collecting the parallel incident optical fibers and the parallel receiving optical fibers;
the vertical collecting optical fiber comprises a vertical incidence optical fiber and a vertical receiving optical fiber which are parallel to each other, and a vertical collecting end which is perpendicular to the vertical incidence optical fiber and the vertical receiving optical fiber respectively, the vertical incidence optical fiber is used for collecting a light source, the vertical receiving optical fiber is used for leading out fluorescence, and the vertical collecting end is arranged in the optical fiber interface and is used for collecting the vertical incidence optical fiber and the vertical receiving optical fiber.
2. The fluorescence quantitative analyzer of claim 1, wherein the parallel collection end is composed of a parallel incident optical fiber at the center and a parallel receiving optical fiber uniformly surrounding the parallel incident optical fiber.
3. The fluorescence quantitative analyzer of claim 1, wherein the vertical collecting end is composed of a vertical incidence optical fiber at the center and a vertical receiving optical fiber uniformly surrounding the outside of the vertical incidence optical fiber.
4. The fluorescence quantitative analyzer of claim 2 or 3, further comprising a fiber bundle detachably connected to the fiber interface, wherein one end of the fiber bundle is connected to the collecting end of the collecting fiber through a connector, and the other end of the fiber bundle is connected to the fiber probe through a connector.
5. The fluorescence quantitative analyzer of claim 4, wherein the coupling assembly comprises a first convex lens, a narrow band filter and a second convex lens coaxially arranged.
6. The fluorescence quantitative analyzer of claim 5, wherein the test strip is provided with a detection band for capturing antigen-antibody complex, a control band for enriching free fluorescent marker, and a two-dimensional code for recording number.
7. The fluorescence quantitative analyzer of claim 6, wherein a circuit board is further disposed in the body, the circuit board comprises a main control chip, an amplifying and filtering circuit, a fluorescence sensor, a light source driving circuit, and an ID chip, a power circuit, an a/D conversion circuit, a timing driving circuit, a synchronous control circuit, and a motor driving circuit, which are respectively connected to the main control chip, wherein:
the main control chip is respectively connected with the liquid crystal screen, the keyboard and the thermal printer;
the main control chip acquires the information of the fluorescence sensor through the A/D conversion circuit and the amplifying and filtering circuit in sequence;
the fluorescence sensor is connected with the time sequence driving circuit;
the main control chip drives the light source to work through the synchronous control circuit and the light source driving circuit in sequence;
the main control chip drives the stepping motor to control through a click driving circuit;
the main control chip is connected with the positive electrode and the negative electrode in the battery box through a power circuit.
8. The fluorescence quantitative analyzer of claim 7, wherein the light source comprises a laser light source, a xenon lamp light source.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810248662.9A CN108181290B (en) | 2018-03-24 | 2018-03-24 | Fluorescent quantitative analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810248662.9A CN108181290B (en) | 2018-03-24 | 2018-03-24 | Fluorescent quantitative analyzer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108181290A CN108181290A (en) | 2018-06-19 |
| CN108181290B true CN108181290B (en) | 2020-07-10 |
Family
ID=62553757
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810248662.9A Active CN108181290B (en) | 2018-03-24 | 2018-03-24 | Fluorescent quantitative analyzer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108181290B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL2033323B1 (en) * | 2022-04-15 | 2023-11-06 | Shanghai Univ Of Medicine And Health Sciences | Immunofluorescence quantitative analyzer |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109211865A (en) * | 2018-11-17 | 2019-01-15 | 郑州亲和力科技有限公司 | A kind of quantitative fluorescence analysis instrument |
| CN109613229A (en) * | 2018-12-27 | 2019-04-12 | 天津博硕科技有限公司 | A kind of guide tracked fluorescence immunity analyzer |
| CN111077306B (en) * | 2019-12-30 | 2023-08-25 | 长沙协大生物科技有限公司 | Quantitative detection analyzer for fluorescent immunoreagent and detection method thereof |
| CN113640508B (en) * | 2021-07-19 | 2024-02-27 | 安徽桐康医疗科技股份有限公司 | Detection plate for fluorescent immunity quantitative analyzer |
| CN114200131B (en) * | 2021-12-13 | 2023-08-15 | 成都云芯医联科技有限公司 | Detection system for luminous characteristics of luminous beads and preparation method of matched detection test paper |
| CN114689838A (en) * | 2022-05-07 | 2022-07-01 | 江西省科学院微生物研究所(江西省流域生态研究所) | Device for reading immunochromatography test strip in high-throughput manner |
| CN117929345B (en) * | 2024-03-22 | 2024-07-02 | 北京万泰生物药业股份有限公司 | Handheld detection device and fluorescence detection system |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101082584A (en) * | 2007-06-28 | 2007-12-05 | 大连海事大学 | Optical fiber biological sensor |
| WO2008140758A1 (en) * | 2007-05-10 | 2008-11-20 | Pacific Biosciences Of California, Inc. | Methods and systems for analyzing fluorescent materials with reduced autofluorescence |
| CN102305866A (en) * | 2011-05-20 | 2012-01-04 | 山东大学 | Detection device for quickly diagnosing acute myocardial infarction |
| CN103091486A (en) * | 2011-11-01 | 2013-05-08 | 马义才 | Test strip detection system |
| CN106990086A (en) * | 2017-06-06 | 2017-07-28 | 中国人民大学 | A kind of multichannel Evanescent Wave All-fiber biology sensor |
-
2018
- 2018-03-24 CN CN201810248662.9A patent/CN108181290B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008140758A1 (en) * | 2007-05-10 | 2008-11-20 | Pacific Biosciences Of California, Inc. | Methods and systems for analyzing fluorescent materials with reduced autofluorescence |
| CN101082584A (en) * | 2007-06-28 | 2007-12-05 | 大连海事大学 | Optical fiber biological sensor |
| CN102305866A (en) * | 2011-05-20 | 2012-01-04 | 山东大学 | Detection device for quickly diagnosing acute myocardial infarction |
| CN103091486A (en) * | 2011-11-01 | 2013-05-08 | 马义才 | Test strip detection system |
| CN106990086A (en) * | 2017-06-06 | 2017-07-28 | 中国人民大学 | A kind of multichannel Evanescent Wave All-fiber biology sensor |
Non-Patent Citations (1)
| Title |
|---|
| 便携式定量荧光免疫分析仪;谢梦圆 等;《仪表技术与传感器》;20141215;第25-27页、图1-4 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL2033323B1 (en) * | 2022-04-15 | 2023-11-06 | Shanghai Univ Of Medicine And Health Sciences | Immunofluorescence quantitative analyzer |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108181290A (en) | 2018-06-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108181290B (en) | Fluorescent quantitative analyzer | |
| CN103675053B (en) | A kind of local electrochemistry imaging test system based on surface plasma resonance | |
| JP2021527822A (en) | Variable multiplexing switches for detector arrays, systems, and how to use them | |
| CN209673807U (en) | A kind of Portable fluorescence immunity analysis instrument | |
| CN110174509A (en) | A kind of spectral analysis device | |
| CN202189010U (en) | Optical detection system for spectrophotometer of automatic biochemistry analyzer | |
| US11959849B2 (en) | Flow cytometers including light collection enhancers, and methods of using the same | |
| CN207908516U (en) | A kind of spectral analysis device | |
| US11994458B2 (en) | Integrated light interrogation modules and methods of use thereof | |
| KR20200040417A (en) | Fluorescence reader device and process in the form of insertion strip for the quantitative measurements | |
| CN207164065U (en) | A kind of Immunofluorescence test device | |
| CN216208536U (en) | Detection unit suitable for portable hair drug detector | |
| KR101764704B1 (en) | Apparatus for Measuring Toxin | |
| CN215116301U (en) | Optical detection system and analysis device of immunofluorescence analyzer | |
| JP2898489B2 (en) | Equipment for spectrophotometric analysis | |
| US20210341380A1 (en) | Flow cytometers including laser assessors, and methods for using the same | |
| CN212340979U (en) | Portable miniature intelligent biochemical analyzer | |
| CN211292604U (en) | Portable gold mark detector based on silicon photocell | |
| CN112630204A (en) | Hand-held fluorescence detector | |
| CN108982389B (en) | Automatic background and reference correction system and method for visible and near infrared spectrums | |
| CN110806399A (en) | Portable fluorescence immunoassay quantitative analyzer | |
| CN219496160U (en) | Water pollution optical detection device based on ESP32 microcontroller | |
| CN206648953U (en) | A kind of fluorimetric analysis device | |
| CN214408690U (en) | Hand-held fluorescence detector | |
| CN213715050U (en) | LED multi-wavelength fluorescence light path debugging device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Wang Bi Inventor after: Jiang Liling Inventor after: Li Jing Inventor after: Shao Xiaoshan Inventor before: Wang Bi Inventor before: Jiang Liling Inventor before: Li Jing |
|
| CB03 | Change of inventor or designer information | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |