CN206648953U - A kind of fluorimetric analysis device - Google Patents
A kind of fluorimetric analysis device Download PDFInfo
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- CN206648953U CN206648953U CN201720232283.1U CN201720232283U CN206648953U CN 206648953 U CN206648953 U CN 206648953U CN 201720232283 U CN201720232283 U CN 201720232283U CN 206648953 U CN206648953 U CN 206648953U
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Abstract
The utility model discloses a kind of fluorimetric analysis device, the device includes power supply module, excitation source module, fluoroscopic examination opto-electronic conversion module, controls analysis module, data storage module and display control module；The utility model uses more excitation sources of rational deployment to provide bigger exciting light for sample to be detected, lifting reaches the excitating light strength on sample to be tested, quickly quantitative detection and analysis can be carried out to excited fluorescence, exciting light needs not move through light transmission piece, when exciting the fluorescence to be detected by Photoelectric Detection module on sample, also without passing through optical filter again, light transmission piece, the part such as condenser or grating filters and reduced interference, both light path design is simplified, reduce the difficulty of assembling, also significantly reduce production cost simultaneously, core light path module is solved to optical filter, the Dependence Problem of collector lens and anti-dazzling screen.
Description
Technical field：
It the utility model is related to technical field of fluorescence detection, and in particular to a kind of fluorimetric analysis device.
Background technology：
The method of fluoroscopic examination is that the glimmering of fixed wave length can be produced under special wavelength light source excitation using analyte
The characteristic of light carries out qualitative and quantitative detecting analysis to it.This method is convenient and swift, be generally also provided with higher sensitivity and
Selectivity, so it is readily applied to real-time or in situ detection.
Common dry type immunofluorescence technique, main sensitiveness, the specificity of antigen-antibody reaction for utilizing fluorescent technique, profit
Antibody, antigen are detected with quantitative measurement technology, meanwhile, 0.5-200mg/l is dry type immuno-fluorescence assay scope, tool
The advantages such as linear scope is wide, quick, sensitivity is high, and blood volume is few, and pediatric patients, acute disease detection are more applicable.Time point
It is to use lanthanide series labelled antigen or antibody to distinguish fluoroimmunoassay, according to the luminous characteristics of lanthanide chelate, uses the time
Resolution techniques measure fluorescence, while two parameters of Detection wavelength and time carry out signal resolution, can effectively exclude non-specific glimmering
The interference of light, drastically increases sensitivity for analysis.
But the detection mode that in the present circumstance, general fluorescence detection device uses is beaten in measured matter for single excitation source
On, fluorescence is received and detected into 90 degree of opening position from exciting light, carries out sampling analysis, such mode fluorescence receives efficiency
It is low.Meanwhile there is strict demand to the optical filter in light path, collector lens and anti-dazzling screen etc..
Utility model content：
The purpose of this utility model is to provide a kind of fluorimetric analysis device, use more excitation sources of rational deployment for
Sample to be detected provides exciting light, and quickly quantitative detection and analysis can be carried out to excited fluorescence, solves core light path mould
Dependence Problem of the block to optical filter, collector lens and anti-dazzling screen.
The utility model is achieved by the following technical programs：
A kind of fluorimetric analysis device, the device include power supply module, excitation source module, fluoroscopic examination opto-electronic conversion
Module, control analysis module, data storage module and display control module；The excitation source module include two or two with
On use with one drive circuit and into it is specific to firing angle degree and ensure reach overlap focus on excitation source, the exciting light
Source is LASER Light Source or UV LED light sources；Fluoroscopic examination opto-electronic conversion module is maintained at same plane with excitation source module axle center
On；Fluoroscopic examination opto-electronic conversion module is fixed on core light path device medium with excitation source module according to fixed angle；It is glimmering
Light detection photoelectricity modulus of conversion group is located at the surface of the coincidence focal zone of excitation source module；The core light path device medium
Further respectively have the path channels of exciting light and fluorescence；Excitation source module overlaps focal zone and set on core light path device medium
There is special gap, the underface in the special gap is sample to be tested area；The fluoroscopic examination opto-electronic conversion module includes signal
Amplifying circuit and analog-to-digital conversion module, the control analysis module include DSP core control panel, stepper motor and drive circuit；
After sample to be tested is sent into detection zone, the coincidence focal length exciting light of excitation source module passes through the special of core light path device medium
Interstitial transmissive carries out exciting the light irradiation specific time to be measured to produce to sample surface to be detected to the signaling zone of sample to be tested
Fluorescence, the special gap of fluorescence to be measured through core light path device medium are received by fluoroscopic examination opto-electronic conversion module, fluorescence inspection
Light-metering electricity conversion module obtains fluorescence signal and is converted into electric signal, processing of the signal by control analysis module, is saved in
Data storage module and display control module is sent to provide corresponding measurement result.
The excitation source and horizontal plane of excitation source module work angular range between 30 degree and 60 degree.
Horizontal layout angle, θ between excitation source module excitation source, can be according to formula：The ÷ n degree of θ=360 obtains, wherein
N is excitation source quantity.
As excitation source quantity n=2, θ=180 degree；As excitation source quantity n=3, θ=120 degree；As n=4,
θ=90 degree；As n=5, θ=72 degree.
Especially, the fluoroscopic examination opto-electronic conversion module is selected from photomultiplier and photodiode.
Described two or more than two LASER Light Source or UV LED light sources multiple light courcess use same one drive circuit, excitation wave
Long identical, focusing illumination, there is provided bigger exciting light, lifting reach the excitating light strength on sample to be tested, and then energy on sample
The fluorescence intensity inspired is also bigger.
The light source direct projection of the excitation source module is on sample to be tested, it is not necessary to by light transmission piece, is excited on sample
When fluorescence is detected by Photoelectric Detection module, it is not required that again by optical filter, light transmission piece, the part such as condenser or grating filters
Disturbed with reducing, both simplify light path design, reduce the difficulty of assembling, while also significantly reduce production cost.
The beneficial effects of the utility model are as follows：The utility model uses more excitation sources of rational deployment as test sample to be checked
The bigger exciting light of this offer, lifting reach the excitating light strength on sample to be tested, and it is quickly fixed that excite fluorescence can be carried out
Amount detection and analysis, exciting light needs not move through light transmission piece, when exciting the fluorescence to be detected by Photoelectric Detection module on sample, also not
Needing to pass through optical filter again, light transmission piece, the part such as condenser or grating filters and reduced interference, both simplifies light path design,
The difficulty of assembling is reduced, while also significantly reduces production cost, solves core light path module to optical filter, collector lens
With the Dependence Problem of anti-dazzling screen.
Brief description of the drawings：
Fig. 1 is double light source optical path structural side views of the fluorimetric analysis device described in embodiment 1；
Wherein, the 1, first excitation source, the 2, second excitation source, 3, fluoroscopic examination opto-electronic conversion module, 4, special gap.
Fig. 2 is double light source optical path structure top views of the fluorimetric analysis device described in embodiment 1；
Fig. 3 is the light channel structure top view of three light sources of the fluorimetric analysis device described in embodiment 2；
Fig. 4 is the light channel structure top view of four light sources of the fluorimetric analysis device described in embodiment 3.
Embodiment：
It is to further explanation of the present utility model, rather than to limitation of the present utility model below.
Embodiment 1：Excitation source module includes two excitation sources
A kind of fluorimetric analysis device, the device include power supply module, excitation source module, fluoroscopic examination opto-electronic conversion
Module 3, control analysis module, data storage module and display control module；As shown in the figures 1 and 2, the excitation source module bag
Include two uses with one drive circuit and into it is specific to firing angle degree and ensure reach overlap focus on excitation source：First excites
The excitation source 2 of light source 1 and second, the first excitation source 1 and the second excitation source 2 and horizontal plane work angular range be 30 degree and
Between 60 degree；Especially, first excitation source and the second excitation source are all LASER Light Source or UV LED light sources, and first swashs
The excitation source 2 of light emitting source 1 and second is placed on the both sides of fluoroscopic examination opto-electronic conversion module 3.Excitation source module excitation source
Between horizontal layout angle, θ=180 degree (as shown in Figure 2).Fluoroscopic examination opto-electronic conversion module 3 is protected with excitation source module axle center
Hold at grade；Fluoroscopic examination opto-electronic conversion module 3 is fixed on core light path with excitation source module according to fixed angle
On device medium；Fluoroscopic examination opto-electronic conversion module 3 is located at the surface of the coincidence focal zone of excitation source module；The core
Heart light path device carrier further respectively has the path channels of exciting light and fluorescence；Excitation source module on core light path device medium
Overlap focal zone and be provided with special gap 4, the underface in the special gap 4 is sample to be tested area；The fluoroscopic examination photoelectricity
Conversion module includes signal amplification circuit and analog-to-digital conversion module, and the control analysis module includes DSP core control panel, stepping
Motor and drive circuit；After sample to be tested is sent into detection zone, the coincidence focal length exciting light of excitation source module passes through core light
The special gap 4 of road device medium projects sample surface to be detected, and the signaling zone of sample to be tested is carried out exciting light irradiation special
The fixed time to produce fluorescence to be measured, is stopped, fluorescence to be measured is through core light path device medium after excitation source module
Special gap 4 by fluoroscopic examination opto-electronic conversion module receive, fluoroscopic examination opto-electronic conversion module obtain fluorescence signal simultaneously convert
For electric signal, processing of the signal by control analysis module, control analysis module will carry out data by DSP core control panel
Handle and control the change in displacement of stepper motor and its drive circuit drives the whole fluoroscopic examination opto-electronic conversion module in Fig. 1 to enter
Line position is moved and detected, while controls analysis module to be responsible for storing data into data storage module and being sent to display control module showing
Show on screen to provide corresponding measurement result.Analysis module is controlled to control stepper motor by DSP core control panel
Change in displacement starts to prepare with detection process for exciting for fluorescence next time.Exciting for fluorescence will be held with detection process by circulation
OK, to complete the whole detection of sample to be tested signaling zone.
Embodiment 2：Excitation source module includes three excitation sources
Reference implementation example 1, difference is：Excitation source module includes three excitation sources, and excitation source module swashs
Horizontal layout angle, θ=120 degree (as shown in Figure 3) between light emitting source.
Embodiment 3：Excitation source module includes four excitation sources
Reference implementation example 1, difference is：Excitation source module includes four excitation sources, and excitation source module swashs
Horizontal layout angle, θ=90 degree (as shown in Figure 4) between light emitting source.
Claims (4)
1. a kind of fluorimetric analysis device, it is characterised in that the device includes power supply module, excitation source module, fluorescence inspection
Light-metering electricity conversion module, control analysis module, data storage module and display control module；The excitation source module includes two
Individual or more than two uses with one drive circuit and into it is specific to firing angle degree and ensure reach overlap focus on excitation source,
The excitation source is LASER Light Source or UV LED light sources；Fluoroscopic examination opto-electronic conversion module is kept with excitation source module axle center
At grade；Fluoroscopic examination opto-electronic conversion module is fixed on core light path device with excitation source module according to fixed angle
On carrier；Fluoroscopic examination opto-electronic conversion module is located at the surface of the coincidence focal zone of excitation source module；The core light
Road device medium further respectively has the path channels of exciting light and fluorescence；Excitation source module overlaps on core light path device medium
Focal zone is provided with special gap, and the underface in the special gap is sample to be tested area；The fluoroscopic examination opto-electronic conversion mould
Group include signal amplification circuit and analog-to-digital conversion module, the control analysis module including DSP core control panel, stepper motor and
Drive circuit；After sample to be tested is sent into detection zone, the coincidence focal length exciting light of excitation source module passes through core light path device
The special interstitial transmissive of carrier carries out exciting the light irradiation specific time to sample surface to be detected to the signaling zone of sample to be tested
To produce fluorescence to be measured, the special gap of fluorescence to be measured through core light path device medium is connect by fluoroscopic examination opto-electronic conversion module
Receive, fluoroscopic examination opto-electronic conversion module obtains fluorescence signal and is converted into electric signal, place of the signal by control analysis module
Reason, it is saved in data storage module and is sent to display control module to provide corresponding measurement result.
2. fluorimetric analysis device according to claim 1, it is characterised in that the excitation source of excitation source module with
Horizontal plane works angular range between 30 degree and 60 degree.
3. fluorimetric analysis device according to claim 1 or 2, it is characterised in that excitation source module excitation source
Between horizontal layout angle, θ, can be according to formula：The ÷ n degree of θ=360 obtains, and wherein n is excitation source quantity.
4. fluorimetric analysis device according to claim 1 or 2, it is characterised in that the fluoroscopic examination opto-electronic conversion
Module is selected from photomultiplier and photodiode.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720232283.1U CN206648953U (en) | 2017-03-10 | 2017-03-10 | A kind of fluorimetric analysis device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720232283.1U CN206648953U (en) | 2017-03-10 | 2017-03-10 | A kind of fluorimetric analysis device |
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| CN206648953U true CN206648953U (en) | 2017-11-17 |
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| CN201720232283.1U Expired - Fee Related CN206648953U (en) | 2017-03-10 | 2017-03-10 | A kind of fluorimetric analysis device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110726704A (en) * | 2019-10-11 | 2020-01-24 | 北京茂泽科技有限公司 | Full-automatic multi-wavelength angle excitation fluorescence luminosity detection analyzer |
-
2017
- 2017-03-10 CN CN201720232283.1U patent/CN206648953U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110726704A (en) * | 2019-10-11 | 2020-01-24 | 北京茂泽科技有限公司 | Full-automatic multi-wavelength angle excitation fluorescence luminosity detection analyzer |
| CN110726704B (en) * | 2019-10-11 | 2024-04-12 | 北京茂泽科技有限公司 | Full-automatic multi-wavelength angle excitation fluorescence photometry detection analyzer |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171117 |