CN208607236U - A kind of fluoroimmunoassay device - Google Patents
A kind of fluoroimmunoassay device Download PDFInfo
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- CN208607236U CN208607236U CN201821037137.4U CN201821037137U CN208607236U CN 208607236 U CN208607236 U CN 208607236U CN 201821037137 U CN201821037137 U CN 201821037137U CN 208607236 U CN208607236 U CN 208607236U
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Abstract
This application discloses a kind of fluoroimmunoassay device, described device includes: light source, exciting light optical fiber, transmitting light optical fiber, light intensity test device, movement mechanism, sample stage；The light source and the exciting light fiber coupling, the transmitting light optical fiber is coupled with the light intensity test device, the first segment of the exciting light optical fiber is disposed adjacent with the second segment of the transmitting light optical fiber, and the first segment includes light-emitting window, and the second segment includes light inlet；The sample stage drives the light-emitting window and the light inlet successively to pass through the same target position on the target area of the chromatographic test paper for placing chromatographic test paper, the movement mechanism；The exciting light that the light source generates is irradiated to the target position by the light-emitting window, and to excite the fluorescent material of the target location, the transmitting light of generation is transmitted to the light intensity test device by the transmitting light optical fiber；The light intensity test device is used to determine the signal strength of the transmitting light.
Description
Technical field
The utility model relates to bioassay field more particularly to a kind of fluoroimmunoassay devices.
Background technique
With the fast development of medical technology, the analysis to index a certain in blood can be passed through to the tentative diagnosis of disease
Obtain preliminary conclusion.Immediately detection (ponit of care testing, POCT) is that one kind can be quickly obtained test result
Clinical assay, currently, the detection method that generally utilizes of POCT is enzyme immunoassay (EIA), precision, poor repeatability, linearly
Range is relatively narrow.
Utility model content
In view of the above problems, the utility model is proposed to overcome the above problem in order to provide one kind or at least partly solve
The certainly fluoroimmunoassay device of the above problem.
The embodiment of the present application provides a kind of fluoroimmunoassay device, and described device includes:
Light source, exciting light optical fiber, transmitting light optical fiber, light intensity test device, movement mechanism, sample stage；
The light source and the exciting light fiber coupling, the transmitting light optical fiber are coupled with the light intensity test device, institute
The first segment for stating exciting light optical fiber is disposed adjacent with the second segment of the transmitting light optical fiber, and the first segment includes light-emitting window, institute
Stating second segment includes light inlet；
The sample stage is for placing chromatographic test paper, and the movement mechanism is for driving the exciting light optical fiber and described
Emit light fibre movement, and/or driving sample stage movement so that the light-emitting window and the light inlet successively pass through it is described
Same target position on the target area of chromatographic test paper；
The exciting light that the light source generates is irradiated to the target position by light-emitting window injection, to excite the mesh
Fluorescent material at cursor position, generates transmitting light, and the transmitting light enters the transmitting light optical fiber, transmission by the light inlet
To the light intensity test device；
The light intensity test device is used to determine the fluorescence signal intensity of the transmitting light.
Optionally, the diameter of the transmitting light optical fiber is greater than or equal to the diameter of the exciting light optical fiber.
Optionally, the light-emitting window is less than the light inlet to the chromatographic test paper to the distance between the chromatographic test paper
The distance between.
Optionally, the line direction between the center and target's center of the light-emitting window, with the light-emitting window and it is described enter
The moving direction of optical port is parallel, the target's center for the light inlet center the light-emitting window throwing in the plane
Shadow.
Optionally, described device further include: one or more receives light optical fiber, perpendicular to incident light optical fiber movement side
It is arranged side by side to setting, and with the incident light optical fiber, for receiving the transmitting light.
Optionally, the transmitting light optical fiber periphery is wrapped up by aluminium foil or goldleaf.
Optionally, the diameter of the transmitting light optical fiber and the diameter ratio of the exciting light optical fiber are more than or equal to 1 and are less than
Equal to 5.
Optionally, the movement mechanism drives the exciting light optical fiber and the transmitting light optical fiber relative to the chromatography
The movement velocity of test paper is adapted to the service life of target range and the fluorescent material, and the target range is the excitation light
The distance between fine and described transmitting light optical fiber.
By one or more technical solution of the application, the application has the advantages that or advantage:
Fluoroimmunoassay device provided by the embodiments of the present application, including light source, exciting light optical fiber, transmitting light optical fiber, light
Strong detection device, movement mechanism, sample stage；The light source and the exciting light fiber coupling, the transmitting light optical fiber with it is described
Light intensity test device coupling, the first segment of the exciting light optical fiber is disposed adjacent with the second segment of the transmitting light optical fiber, described
First segment includes light-emitting window, and the second segment includes light inlet；The sample stage wraps on chromatographic test paper for placing chromatographic test paper
Include the sample marked by fluorescent material；The movement mechanism is for driving the exciting light optical fiber and the transmitting light optical fiber
Movement, and/or the driving sample stage movement, so that the light-emitting window and the light inlet successively pass through the chromatographic test paper
Same target position on target area；When the light-emitting window passes through the target position, the exciting light of the light source generation
It is irradiated to the target position by light-emitting window injection, to excite the fluorescent material of the target location, generates transmitting
Light, when the light inlet passes through the target position, the transmitting light enters the transmitting light optical fiber by the light inlet,
It is transmitted to the light intensity test device, to realize time-resolved fluoroimmunoassay；The light intensity test device is for determining institute
The fluorescence signal intensity of transmitting light is stated, and then determines the concentration of tested index.Fluoroimmunoassay provided by the embodiments of the present application
Structure in device can be realized time-resolved fluoroimmunoassay, and then significantly improve the precision of tested sample detection and steady
It is qualitative.
Detailed description of the invention
By reading the following detailed description of the preferred embodiment, various other advantages and benefits are common for this field
Technical staff will become clear.The drawings are only for the purpose of illustrating a preferred embodiment, and is not considered as to the application
Limitation.And throughout the drawings, the same reference numbers will be used to refer to the same parts.In the accompanying drawings:
Fig. 1 is a kind of schematic diagram of fluoroimmunoassay device shown in the embodiment of the present application；
Fig. 2 is the schematic diagram of another fluoroimmunoassay device for implementing to exemplify of the application；
Fig. 3 is that there are angles with the direction of motion for the center of light-emitting window and the line direction of target's center in the embodiment of the present application
Schematic diagram；
Fig. 4 is the position view that light optical fiber is received in the embodiment of the present application；
Fig. 5 is the block diagram of another fluoroimmunoassay device provided by the embodiments of the present application.
Specific embodiment
The present embodiment discloses a kind of fluoroimmunoassay device, which includes: light source, exciting light optical fiber, transmitting light light
Fibre, light intensity test device, movement mechanism, sample stage；The light source and the exciting light fiber coupling, the transmitting light optical fiber and
The light intensity test device coupling, the first segment of the exciting light optical fiber are disposed adjacent with the second segment of the transmitting light optical fiber,
The first segment includes light-emitting window, and the second segment includes light inlet；The sample stage is for placing chromatographic test paper, the movement
Mechanism is used to drive the exciting light optical fiber and the transmitting light fibre movement, and/or the driving sample stage movement, so that
The light-emitting window and the light inlet successively pass through the same target position on the target area of the chromatographic test paper；The light source
The exciting light of generation is irradiated to the target position by light-emitting window injection, to excite the fluorescence of the target location
Matter generates transmitting light, and the transmitting light enters the transmitting light optical fiber by the light inlet, is transmitted to the light-intensity test dress
It sets；The light intensity test device is used to determine the fluorescence signal intensity of the transmitting light.
Technical scheme is described in detail below by attached drawing and specific embodiment, it should be understood that the application
Specific features in embodiment and embodiment are the detailed description to technical scheme, rather than to present techniques
The restriction of scheme, in the absence of conflict, the technical characteristic in the embodiment of the present application and embodiment can be combined with each other.
Embodiment one
As shown in Figure 1, for a kind of schematic diagram of fluoroimmunoassay device shown in the embodiment of the present application, the device packet
It includes: light source 11, exciting light optical fiber 12, transmitting light optical fiber 13, light intensity test device 14, movement mechanism 15, sample stage 16；Light source 11
Coupled with exciting light optical fiber 12, transmitting light optical fiber 13 coupled with light intensity test device 14, the first segment 121 of exciting light optical fiber 12 and
The second segment 131 of transmitting light optical fiber 13 is disposed adjacent, and first segment 121 includes light-emitting window 122, and second segment 131 includes light inlet
132；Sample stage 16 is for placing chromatographic test paper, and movement mechanism 15 is for driving exciting light optical fiber 12 and transmitting light optical fiber 13
Movement, and/or driving sample stage 16 move, so that light-emitting window 122 and light inlet 132 successively pass through the target of the chromatographic test paper
Same target position on region；The exciting light that light source 11 generates is irradiated to the target position by the injection of light-emitting window 122,
To excite the fluorescent material of the target location, transmitting light is generated, the transmitting light enters transmitting light light by light inlet 132
Fibre 13, is transmitted to light intensity test device 14；Light intensity test device 14 is used to determine the fluorescence signal intensity of the transmitting light.
In the embodiment of the present application, light source 11 is that can excite light source, such as ultraviolet lamp, laser, LED of fluorescent material etc.,
In one embodiment, light source 11 is that energy is strong, LED ultraviolet lamp of pulse width.Light source 11 is coupled with exciting light optical fiber 12 to be referred to,
The exciting light that light source 11 issues can inject exciting light optical fiber 12 directly or by other component.Emit light optical fiber 13 and light intensity is examined
It surveys the coupling of device 14 to refer to, the transmitting light that emitted optical fiber 13 transmits can be incident on light intensity inspection directly or by other component
It surveys on device 14.Light intensity test device can be selected according to actual needs, such as photomultiplier tube, photodiode, silicon
Photocell etc..
As shown in Fig. 2, for the schematic diagram of another fluoroimmunoassay device for implementing to exemplify of the application, in Fig. 2, light
The exciting light that source 11 generates enters exciting light optical fiber 12 by lens group 21, optical filter 22.Wherein, lens group 21 and optical filter
22 can be selected according to actual needs, and by taking light source 11 is LED ultraviolet lamp as an example, lens group 21 can be ultraviolet lens group,
Optical filter 22 can be ultraviolet filter, and the exciting light that LED ultraviolet lamp generates obtains ultraviolet after being filtered by ultraviolet lens group
Ultraviolet light is gathered one end of exciting light optical fiber by light, ultraviolet lens group, goes out light by exciting light optical fiber 12 by fiber optic conduction
Mouth 122 projects.In one embodiment, plane where the light-emitting window of exciting light optical fiber 12, the light inlet institute for emitting light optical fiber 13
It is parallel with plane where chromatographic test paper in plane.
In the embodiment of the present application, movement mechanism 15 can drive exciting light optical fiber 12 and transmitting light optical fiber 13 relative to layer
The target area for analysing test paper is mobile, it should be understood that relative movement can be movement mechanism 15 and drive exciting light optical fiber 12 and hair
The movement of light optical fiber 13 is penetrated, the sample stage 16 for placing chromatographic test paper is motionless, is also possible to exciting light optical fiber 12 and transmitting light optical fiber 13
Motionless, the movement of sample stage 16 of chromatographic test paper is placed in the driving of movement mechanism 15 or movement mechanism drives exciting light optical fiber simultaneously
12 move with transmitting light optical fiber 13 and sample stage 16.
Target area can be all areas of chromatographic test paper, be also possible to the partial region of chromatographic test paper, for example, target
Region is the test zone including chromatographic test paper T band.In one embodiment, mark is instilled in the sample pad of chromatographic test paper
Note has the sample of long-life phosphors substance, and sample can be upper after whole blood, serum, blood plasma, urine or other body fluid, or dilution
State sample, the biological substance for including in sample can be can be by macro-molecular protein that chromatography scheme detects, small molecule half
One of antigen, nucleic acid or oligonucleotides are a variety of.Sample can chromatograph forward on chromatographic test paper through capillary action, mark
Note has the biological substance of long-life phosphors substance that can be fixed on T band, when the irradiation by exciting light, forms transmitting light,
That is fluorescence.Emit light and transmitting light optical fiber 13 is entered by light inlet 132, is transmitted to light intensity test device 14, passes through light-intensity test
Device 14 detects the fluorescence intensity for the transmitting light that long-life phosphors substance is formed, and can determine that label has substance
The concentration of biological substance.
It should be understood that there may also be fluorescent materials for chromatographic test paper itself, when exciting light is irradiated on chromatographic test paper,
In addition to long-life phosphors substance forms transmitting light, other fluorescent materials also can be excited to form transmitting light, and generally, these are glimmering
The service life of stimulative substance is shorter than the service life of long-life phosphors substance, and the fluorescence of generation, which also can comparatively fast decay, to be finished.Therefore, the application is real
It applies in example, light-emitting window 122 and light inlet 132 reach target using light-emitting window 122 and light inlet 132 successively by target position
The time difference of position so that short life fluorescent material excite the transmitting light to be formed light inlet 132 reach target position before just
Decaying finishes, and the transmitting light that long-life phosphors substance is formed is received when light inlet 132 reaches target position.Effectively avoid
The interference of other fluorescent materials improves the accuracy of detection long-life phosphors intensity.
In the embodiment of the present application, movement mechanism 15 drives exciting light optical fiber 12 to move with respect to chromatographic test paper, can make to excite
Light carries out point by point scanning or progressive scan to target area, in order to be scanned to entire target area, implements at one
In example, movement mechanism 15 drives exciting light optical fiber 12 and transmitting light optical fiber 13 does continuous back and forth movement, or driving sample stage does and connects
Continuous motion in one dimension, makes chromatographic test paper do continuous feed motion, completes the scanning to entire target area.
Light intensity test device 14 may include the elements such as photodiode, silicon photocell, photomultiplier tube, for believing light
Number it is changed into electric signal, can also includes controller, for determining light intensity according to electric signal.In one embodiment, exciting light
Point by point scanning is carried out to target area, after completing the scanning to target area, light intensity test device 14 believes the light received
It number being handled, the sequential test value of available one intensive test point, the test point corresponds to each excitation point of exciting light,
Matched curve is drawn according to sequential test value, and calculates the area of the curve wave crest, so that it is determined that the intensity of fluorescence signal.
Optionally, when the fluorescent material on the target position include the first fluorescent material and the second fluorescent material,
And the service life of first fluorescent material be higher than second fluorescent material service life when, movement mechanism 15 can drive out light
Mouth 122 and light inlet 132 are mobile relative to the target area with pre-set velocity；In light-emitting window 122 and light inlet 132 with described pre-
If speed successively passes through the target position, the exciting light can excite the long-life phosphors substance of the target location
The first transmitting light is formed, and excites short life fluorescent material formation the second transmitting light of the target location, described first
Emit light and enter the transmitting light optical fiber when light inlet 132 is by the target position, the second transmitting light is in light inlet
Decay before the 132 arrival target positions and has finished.
In the embodiment of the present application, long-life phosphors substance can according to need to be selected, in one embodiment, long
Service life fluorescent material may include lanthanide series, for example, rare earth ion chelate or include rare earth ion chelate macromolecule
Fluorescent nano particles.Pre-set velocity can be default value, can also be determined according to fluorescence labeling material.For example, with fluorescence
Matter be lanthanide series for, due to lanthanide series transmitting light perdurabgility be 100~1000 microseconds, movement mechanism 15
Drive exciting light optical fiber light-emitting window 122 and transmitting light optical fiber light inlet 132 by target position time difference be 100~
1000 microseconds.Within the time difference, common fluorescent has been decayed and has been finished such as the background fluorescence of chromatographic test paper, when light inlet 132
When reaching target position, the transmitting light that only lanthanide series inspires can inject light inlet 132, avoid the dry of background fluorescence
It disturbs.According to the time difference, and according to the distance between light-emitting window 122 and light inlet 132, that is, it can determine that movement mechanism drives out light
The pre-set velocity of mouth 122 and light inlet 132.That is, movement mechanism 15 drives exciting light optical fiber 12 and transmitting light optical fiber 13 opposite
It is adapted in the movement velocity of the chromatographic test paper with the service life of target range and the fluorescent material, the target range is sharp
The distance between emitting optical fiber 12 and transmitting light optical fiber 13.
Optionally, the first segment 121 of exciting light optical fiber 12 is disposed adjacent with the second segment 131 of transmitting light optical fiber 13, and first
Section 121 includes light-emitting window 122, and second segment 131 includes light inlet 132.
As shown in Fig. 2, first segment 121 is disposed adjacent with second segment 131 along with the direction of chromatographic test paper relative motion, one
In a embodiment, first segment 121 and second segment 131 can be fixed together by the modes such as pasting, binding, two fiber optic hubs
The distance between the sum of for two fiber radius.So according to the sum of two fiber radius and time difference, then fortune can be determined
The pre-set velocity of motivation structure.It should be understood that the distance between two fiber optic hubs can be greater than or equal to the half of two optical fiber
The sum of diameter needs the thickness in view of aluminium foil for example, when shielding other light using aluminium foil between two optical fiber, and at this time two
The distance between root fiber optic hub is greater than the sum of the radius of two optical fiber.The diameter of exciting light optical fiber 12 and transmitting light optical fiber 13 can
To be selected according to actual needs, in one embodiment, the diameter of exciting light optical fiber 12 and transmitting light optical fiber 13 is
125 microns.
Optionally, the diameter for emitting light optical fiber 13 is greater than or equal to the diameter of exciting light optical fiber 12.Long-life in order to prevent
The exciting light that fluorescent material generates is missed, and transmitting light optical fiber 12 can be done slightly, be expanded the range of receiving of transmitting light optical fiber 12,
In one embodiment, what the diameter by adjusting transmitting light optical fiber 12 was is in apart from 100~900 microns of light inlet center model
Fluorescence in enclosing can enter in transmitting light optical fiber.The diameter of transmitting light optical fiber and exciting light optical fiber can come according to actual needs
It is configured, in one embodiment, the ratio range for emitting light optical fiber and exciting light fibre diameter is 1:1~5:1.
Optionally, light-emitting window 122 to the distance between described chromatographic test paper be less than light inlet 132 to the chromatographic test paper it
Between distance.Emitting light into for common fluorescent substance generation emits light optical fiber in order to prevent, in the embodiment of the present application, Ke Yizeng
Optical port 132 is added and arrives the distance between chromatographic test paper, Lai Yanchang emits light into the distance of light inlet 132, to extend transmitting
Light enters the time of light inlet 132, and common fluorescent is all decayed completely, cannot be introduced into transmitting light optical fiber 13.
It should be understood that since there may be the interference detected to light intensity test device 14 to fluorescence intensity for exciting light, that is,
Exciting light may enter transmitting light optical fiber 13 and be transmitted at light intensity test device 14.In order to avoid the interference of exciting light, this Shen
Exciting light optical fiber 12 and transmitting light optical fiber 13 can please be separated with aluminium foil or goldleaf, as shown in Figure 1, transmitting in embodiment
Light optical fiber periphery includes the aluminium foil that a layer thickness is 1 micron.
In addition, in the embodiment of the present application, optical filtering can also be arranged at light inlet 132 in order to avoid the interference of other light
Piece, to cross other light filtered out other than long-life phosphors.
Optionally, the line direction between the center and target's center of light-emitting window 122, with light-emitting window 122 and light inlet 132
Moving direction it is parallel, the target's center for light inlet 132 center light-emitting window 122 projection in the plane.
Referring to FIG. 3, for the center of light-emitting window and the line direction of target's center and the direction of motion in the embodiment of the present application
There are the schematic diagrames of angle, when the line of the center of light-emitting window 122 and target's center and the direction of motion are not parallel, fluorescent material
Part transmitting light after excitation, which is possible to that light optical fiber can not be launched, to be received, therefore, being capable of whole quilts in order to guarantee to emit light
It receives, needs to keep the center of light-emitting window 122 and the line direction of target's center parallel with the direction of motion, here parallel can be with
It is the angle between line direction and the direction of motion less than a threshold value.
In addition, can be all received to guarantee to emit light, described device further include: one or more receives light light
Fibre is arranged perpendicular to 13 moving direction of incident light optical fiber, and is arranged side by side with incident light optical fiber 13, for receiving the transmitting
Light.
Referring to FIG. 4, to receive the position view of light optical fiber in the embodiment of the present application, due to be arranged side by side one or
More piece-root graftings receive light optical fiber 41, can not when the center of light-emitting window 122 and the line direction of target's center and the direction of motion are not parallel
Transmitting light into light inlet 132 can be received by receiving light optical fiber 41.In one embodiment, receive light optical fiber 41 with
Light intensity test device 14 couples, can be by the transmitting optical transport received to light intensity test device 14, and light intensity test device 14
According to the transmitting light that transmitting light optical fiber 13 and reception light light 41 transmit, to determine fluorescence intensity.
Optionally, movement mechanism 15 drives light inlet 132 by any position of the white space in the target area
When, the background fluorescence of any position enters the transmitting light optical fiber by light inlet 132；Light intensity test device 14, is used for
According to the first transmitting light and the background fluorescence, the fluorescent intensity of the target location is determined.
It include the biological substance for being marked with long-life phosphors substance in the target area of chromatographic test paper in the embodiment of the present application
Region further includes having the biological substance region without containing long-life phosphors substance.In one embodiment, target area includes solid
The region for having determined capture probe is capable of fixing the biological substance for being marked with long-life phosphors substance, further includes that unlocked capture is visited
The white space of needle.When light-emitting window 122 and light inlet 132 are by repeatedly moving to target area scanning, capture is being secured
The region of probe receives the transmitting light of long-life phosphors substance formation, and the determining long-life formed with long-life phosphors substance is glimmering
Light signal strength.Since some components of fluoroimmunoassay device itself are also likely to be present fluorescence, that is, there is background fluorescence,
Therefore, when being scanned to target area, it might have background fluorescence in each scanning element and enter transmitting light optical fiber 13,
Interference is generated to long-life phosphors signal.Therefore, in the embodiment of the present application, when being taken multiple scan to white space, to sky
The background fluorescence signal of white region is received, and determines the mean intensity of background fluorescence signal, strong in long-life phosphors signal
The mean intensity that the background fluorescence signal is subtracted in degree obtains the intensity of final long-life phosphors signal, so that result is more
Accurately.
Embodiment two
As shown in figure 5, for the block diagram of another fluoroimmunoassay device provided by the embodiments of the present application, which includes
Optical system, chromatographic test paper drive system, signal detection and control system.
Optical system, including LED ultraviolet lamp, ultraviolet filter, ultraviolet lens group, exciting light optical fiber, transmitting light optical fiber, light
Electric multiplier tube.Wherein, for LED ultraviolet lamp as light source, the light of generation gathers one end of exciting light optical fiber by ultraviolet lens group,
Ultraviolet filter obtains ultraviolet light after filtering to exciting light, ultraviolet light is passed to by optical fiber and projected by light-emitting window, is irradiated to
Target area on chromatographic test paper.Tested substance on target area is labeled with long-life phosphors substance, in light outside
Under excitation, long-life phosphors substance generates transmitting light, enters transmitting light optical fiber by the light inlet of transmitting light optical fiber, is transmitted to photoelectricity
On the target surface of multiplier tube, photomultiplier tube converts light signals into electric signal.
Chromatographic test paper drive system may include power amplifier, high-precision stepper motor, XY is displaced and optoelectronic switch,
Four components are sequentially connected in series.Chromatographic test paper does continuous motion in one dimension, makes chromatographic test paper under the driving of high-precision stepper motor
Continuous feed motion is done, to realize to the point-by-point of target area or progressive scan.Power amplifier is also connected with single-chip microprocessor MCU
(Microcontroller Unit, micro-control unit), MCU is separately connected RAM, EPROM, keyboard, display, printer and electricity
Source, the excitation optical scanning target area that light source generates after energization, by being sequentially connected in series photomultiplier tube, preamplifier, signal
Amplification and discriminator, A/D conversion circuit will test signal input single-chip microprocessor MCU.
Signal detection and control system, the course of work are as follows: being swashed the target position of the target area of chromatographic test paper
After the excitation that emitting optical fiber light-emitting window projects, light-emitting window is mobile by the example platform for being placed with chromatographic test paper, mobile
To adjacent position, at this point, the long-life phosphors and background fluorescence that generate are through overdamping, when with light-emitting window apart from certain length
When emitting the light inlet arrival target position of light optical fiber, the long-life phosphors in one preset range of light inlet center enter hair
Light optical fiber is penetrated, and enters photomultiplier tube by optical filter, optical signal is become into fluorescence intensity signals, is recorded by single-chip microcontroller
Come, exciting light optical fiber and transmitting light optical fiber are continuously scanned the sample spot on motion path, can constantly carry out the long-life
Fluorescence strength investigation, and short-life fluorescence transmitting light optical fiber light inlet reach before attenuated, not can enter
Optical fiber, thus detect less than, avoid the interference of background fluorescence, realize time-resolved fluorescence detection.
Although the preferred embodiment of the utility model has been described, one of ordinary skilled in the art is once known
Basic creative concept, then additional changes and modifications can be made to these embodiments.So appended claims are intended to explain
Being includes preferred embodiment and all change and modification for falling into the scope of the utility model.
Obviously, it is practical without departing from this can to carry out various modification and variations to the utility model by those skilled in the art
Novel spirit and scope.If in this way, these modifications and variations of the present invention belong to the utility model claims and
Within the scope of its equivalent technologies, then the utility model is also intended to include these modifications and variations.
Claims (8)
1. a kind of fluoroimmunoassay device, which is characterized in that described device includes:
Light source, exciting light optical fiber, transmitting light optical fiber, light intensity test device, movement mechanism, sample stage；
The light source and the exciting light fiber coupling, the transmitting light optical fiber are coupled with the light intensity test device, described to swash
The first segment of emitting optical fiber is disposed adjacent with the second segment of the transmitting light optical fiber, and the first segment includes light-emitting window, and described the
Two sections include light inlet；
The sample stage is for placing chromatographic test paper, and the movement mechanism is for driving the exciting light optical fiber and the transmitting
Light fibre movement, and/or the driving sample stage movement, so that the light-emitting window and the light inlet successively pass through the chromatography
Same target position on the target area of test paper；
The exciting light that the light source generates is irradiated to the target position by light-emitting window injection, to excite the target position
The fluorescent material at place is set, transmitting light is generated, the transmitting light enters the transmitting light optical fiber by the light inlet, is transmitted to institute
State light intensity test device；
The light intensity test device is used to determine the fluorescence signal intensity of the transmitting light.
2. fluoroimmunoassay device according to claim 1, which is characterized in that the diameter of the transmitting light optical fiber is greater than
Or the diameter equal to the exciting light optical fiber.
3. fluoroimmunoassay device according to claim 1, which is characterized in that the light-emitting window to the chromatographic test paper
The distance between be less than described light inlet the distance between to the chromatographic test paper.
4. fluoroimmunoassay device according to claim 1, which is characterized in that in the center and target of the light-emitting window
Line direction between the heart, parallel with the moving direction of the light-emitting window and the light inlet, the target's center enters for described in
The center of optical port the light-emitting window projection in the plane.
5. fluoroimmunoassay device according to claim 1, which is characterized in that described device further include: one or more
Piece-root grafting receives light optical fiber, is arranged perpendicular to the incident light optical fiber moving direction, and is arranged side by side with the incident light optical fiber, is used for
Receive the transmitting light.
6. fluoroimmunoassay device according to claim 1, which is characterized in that transmitting light optical fiber periphery is by aluminium foil
Or goldleaf package.
7. fluoroimmunoassay device according to claim 1, which is characterized in that the diameter of the transmitting light optical fiber and institute
The diameter ratio for stating exciting light optical fiber is more than or equal to 1 and is less than or equal to 5.
8. fluoroimmunoassay device according to claim 1, which is characterized in that the movement mechanism drives the excitation
The movement velocity and target range and the fluorescence of light optical fiber and the transmitting light optical fiber relative to the chromatographic test paper
The service life of matter is adapted to, and the target range is the distance between the exciting light optical fiber and the transmitting light optical fiber.
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| CN201821037137.4U CN208607236U (en) | 2018-06-30 | 2018-06-30 | A kind of fluoroimmunoassay device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110057808A (en) * | 2019-05-27 | 2019-07-26 | 中国人民解放军军事科学院军事医学研究院 | Sample swivel mount and Raman spectrum detector |
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2018
- 2018-06-30 CN CN201821037137.4U patent/CN208607236U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110057808A (en) * | 2019-05-27 | 2019-07-26 | 中国人民解放军军事科学院军事医学研究院 | Sample swivel mount and Raman spectrum detector |
| US11971358B2 (en) | 2019-05-27 | 2024-04-30 | Academy Of Military Medical Sciences | Sample rotating rack and Raman spectrum detector |
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