CN220393747U - Portable isothermal amplification all-in-one machine - Google Patents
Portable isothermal amplification all-in-one machine Download PDFInfo
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- CN220393747U CN220393747U CN202321760137.8U CN202321760137U CN220393747U CN 220393747 U CN220393747 U CN 220393747U CN 202321760137 U CN202321760137 U CN 202321760137U CN 220393747 U CN220393747 U CN 220393747U
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- isothermal amplification
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- detection
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- 238000011901 isothermal amplification Methods 0.000 title claims abstract description 27
- 238000001514 detection method Methods 0.000 claims abstract description 36
- 238000010438 heat treatment Methods 0.000 claims abstract description 23
- 230000005284 excitation Effects 0.000 claims abstract description 17
- 238000004458 analytical method Methods 0.000 claims abstract description 4
- 239000003153 chemical reaction reagent Substances 0.000 claims description 11
- 230000001681 protective effect Effects 0.000 claims description 8
- 238000009413 insulation Methods 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 3
- 238000009434 installation Methods 0.000 abstract 1
- 238000004321 preservation Methods 0.000 description 8
- 150000007523 nucleic acids Chemical class 0.000 description 4
- 102000039446 nucleic acids Human genes 0.000 description 4
- 108020004707 nucleic acids Proteins 0.000 description 4
- 230000003321 amplification Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 238000007397 LAMP assay Methods 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000001823 molecular biology technique Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
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- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The utility model discloses a portable isothermal amplification integrated machine, which comprises a box body with a top cover; the display screen is connected to the box body, and the installation frame is connected to the inside of the box body; the mounting frame is connected with a placement block with a constant temperature heating module, a plurality of fixing grooves are formed in the placement block, light inlet holes are formed in the placement block at positions corresponding to the bottoms of the fixing grooves, and an excitation light source is arranged at the light inlet holes; the position of the placing block corresponding to one side of the bottom of each fixed tank is provided with a detection hole, and the detection hole is provided with a detection module; the box body is internally connected with a control module, the control module is used for controlling the constant temperature heating module, the excitation light source and the detection module to work, collecting and analyzing signals of the detection module in real time, and transmitting analysis results of the signals to the display screen for display. The utility model not only can reduce the volume of the equipment and make the storage and carrying of the equipment more convenient, but also has the advantage of better protection.
Description
Technical Field
The utility model relates to the field of isothermal amplification equipment, in particular to a portable isothermal amplification all-in-one machine.
Background
In recent years, with rapid development of molecular biology techniques, diagnostic methods based on nucleic acid detection have been established in large numbers and widely used in laboratory detection of diseases, and isothermal amplification techniques have emerged in this context. Isothermal amplification is the rapid amplification of nucleic acids by using a specific DNA polymerase at a constant temperature.
The existing nucleic acid isothermal amplification instrument is large in whole generally, and the detection result is troublesome to read. Therefore, ZL202110636900.5 discloses a portable loop-mediated isothermal amplification device and an operation method thereof, which design the whole device to be small and convenient, and adopt a closed light path design to output the interpretation result in the reaction process in real time through an indicator lamp. However, in the light path design of such equipment, the adjustable excitation light source focusing lens, the constant temperature placing groove, the optical filter, the detection focusing lens and the photoelectric sensor structure are required to be sequentially arranged, so that the whole equipment is large and is not easy to store and carry.
Therefore, the conventional isothermal amplification device has the problems of large equipment and difficulty in storage and carrying.
Disclosure of Invention
The utility model aims to provide a portable isothermal amplification all-in-one machine. The utility model has the advantages of smaller equipment and convenient storage and carrying.
The technical scheme of the utility model is as follows: a portable isothermal amplification all-in-one machine comprises a box body, wherein a top cover is arranged at the top end of the box body; a display screen is connected to one side surface of the box body, and a mounting frame is connected to the inside of the box body; the mounting frame is connected with a placement block, and the placement block is provided with a constant-temperature heating module; the placing block is provided with a plurality of fixing grooves for placing reagent tubes, the placing block is provided with light inlet holes corresponding to the positions below the bottoms of the fixing grooves, and the light inlet holes are provided with excitation light sources; the position of the placing block corresponding to one side of the bottom of each fixed tank is provided with a detection hole, and the detection hole is provided with a detection module; the box body is internally connected with a control module, the control module is used for controlling the constant temperature heating module, the excitation light source and the detection module to work, collecting and analyzing signals of the detection module in real time, and transmitting analysis results of the signals to the display screen for display.
In the portable isothermal amplification integrated machine, the constant temperature heating module comprises a heating plate and a temperature sensor which are connected to the placing block; the heating plate and the detection holes are respectively positioned at two sides of the placement block.
In the portable isothermal amplification integrated machine, a heat preservation block is arranged in the top cover, and a baffle connected to the top cover is arranged below the heat preservation block; and a notch for exposing the heat insulation block is arranged on the baffle plate at a position right above the plurality of fixing grooves.
In the portable isothermal amplification all-in-one machine, the top end of the box body is connected with a protective cover plate, and the protective cover plate is provided with placing holes at positions corresponding to each fixing groove.
In the portable isothermal amplification integrated machine, the box body is connected with a power socket and a control button which are electrically connected with the control module.
In the portable isothermal amplification all-in-one machine, the excitation light source comprises a light guide strip fixed on the mounting frame, and one end of the light guide strip is connected with an LED light source; the light guide strip is provided with a light emitting section which is horizontally arranged below all the light inlet holes, and the bottom of the light emitting section is provided with light teeth.
In the portable isothermal amplification all-in-one machine, the detection module is a camera.
In the portable isothermal amplification all-in-one machine, one side of the top cover is hinged on the box body.
Compared with the prior art, the utility model has the advantages that the light inlet hole and the excitation light source are arranged below the fixed groove, the detection hole and the detection module are arranged on one side of the bottom of the fixed groove, the whole light path is changed from a horizontal line to a vertical bending line, the thickness space of the bottom of the placing block is effectively utilized, the minimum width required by the whole equipment is reduced under the condition that the height is not obviously increased, the volume of the whole equipment is reduced, and the whole equipment is convenient to store and carry.
In addition, the utility model realizes constant temperature control through the heating plate and the temperature sensor, improves the traditional design structure, and arranges the heating part arranged at the bottom of the placing block on the side surface of the placing block, thereby arranging the excitation light source at the bottom of the placing block without additionally increasing the height of the whole equipment.
The heat preservation block is arranged in the top cover in a limiting manner through the baffle plate, when the top cover is arranged at the top of the box body, the heat preservation block can be arranged at the top of the reagent tube through the mask, and has a good heat preservation effect on the reagent tube, so that the reagent tube can perform constant-temperature amplification operation well.
The protection apron on top in the box can seal the protection with the inside components and parts of box, and does not influence the detection operation of putting into the fixed slot with the reagent pipe.
The excitation light source can be formed by combining a single LED light source and a light guide strip, so that the occupied space is small; after the LED light source is excited to form a light source, light can be guided to the light emitting section through the light guide strip, and the light emitting section of the light guide strip can emit light upwards due to the fact that the light teeth are arranged at the bottom of the light emitting section, so that light injection operation is achieved. The combined structure of the single LED light source and the light guide strip can simplify the structure of the excitation light source.
Therefore, the utility model not only can reduce the volume of the equipment and make the storage and carrying of the equipment more convenient, but also has the advantage of better protection.
Drawings
FIG. 1 is a schematic diagram of the structure of the present utility model;
FIG. 2 is an exploded view of the present utility model;
FIG. 3 is a schematic view of the structure of the top cover;
FIG. 4 is a schematic structural view of a protective cover plate;
FIG. 5 is a schematic diagram of the placement block and excitation light source configuration;
FIG. 6 is a schematic view of the structure of the placement block;
fig. 7 is a schematic structural diagram of the control module and the camera.
The marks in the drawings are: the LED light source comprises a 1-box body, a 2-top cover, a 3-display screen, a 4-mounting frame, a 5-placement block, a 6-fixing groove, a 7-light inlet hole, an 8-detection hole, a 9-control module, a 10-heating plate, an 11-temperature sensor, a 12-heat preservation block, a 13-baffle, a 14-notch, a 15-protection cover plate, a 16-placement hole, a 17-power socket, an 18-control button, a 19-light guide bar, a 20-LED light source, a 21-luminous section, 22-light teeth and a 23-camera.
Detailed Description
The utility model is further illustrated by the following figures and examples, which are not intended to be limiting.
Examples. The portable isothermal amplification all-in-one machine is shown in figures 1 to 7, and comprises a box body 1, wherein a top cover 2 is arranged at the top end of the box body 1; a display screen 3 is connected to one side surface of the box body 1, and a mounting frame 4 is connected to the inside of the box body 1; a placement block 5 is connected to the mounting frame 4, and a constant-temperature heating module is arranged on the placement block 5; the placing block 5 is provided with a plurality of fixing grooves 6 for placing reagent tubes, the placing block 5 is provided with light inlet holes 7 corresponding to the positions below the bottom of each fixing groove 6, and the light inlet holes 7 are provided with excitation light sources; the position of the placement block 5 corresponding to one side of the bottom of each fixed groove 6 is provided with a detection hole 8, and the detection hole 8 is provided with a detection module; the box body 1 is internally connected with a control module 9, the control module 9 is used for controlling the constant temperature heating module, the excitation light source and the detection module to work, collecting and analyzing signals of the detection module in real time, and transmitting analysis results of the signals to the display screen 3 for display.
The constant temperature heating module comprises a heating plate 10 and a temperature sensor 11 which are connected to the placing block 5; the heating plate 10 and the detection holes 8 are respectively positioned at two sides of the placement block 5; a heat preservation block 12 is arranged in the top cover 2, and a baffle 13 connected to the top cover 2 is arranged below the heat preservation block 12; notches 14 for exposing the heat insulation blocks 12 are arranged on the baffle 13 at positions corresponding to the positions right above the plurality of fixing grooves 6; the top end of the box body 1 is connected with a protective cover plate 15, and a placement hole 16 is arranged on the protective cover plate 15 at a position corresponding to each fixing groove 6; the box body 1 is connected with a power socket 17 and a control button 18 which are electrically connected with the control module 9; the excitation light source comprises a light guide strip 19 fixed on the mounting frame 4, and one end of the light guide strip 19 is connected with an LED light source 20; the light guide strip 19 is provided with a light emitting section 21, the light emitting section 21 is horizontally arranged below all the light inlet holes 7, and the bottom of the light emitting section 21 is provided with light teeth 22; the detection module is a camera 23; one side of the top cover 2 is hinged on the box body 1.
Working principle: opening the top cover 2, and placing the reagent tube to be detected into the fixed groove 6 on the placing block 5 through the placing hole 16 on the protective cover plate 15; subsequently, the top cover 2 is covered, and the heat insulation block 12 positioned at the notch 14 of the baffle 13 on the top cover 2 can be covered on the top of the reagent tube.
The power socket 17 on the box body 1 can provide power, and the detection operation can be started by pressing the control button 18 on the box body 1; the control module 9 (the model of the chip in the control module 9 can be ARMSTM 32) feeds back the temperature of the placement block 5 in real time through the temperature sensor 11, and controls the heating operation of the heating plate 10, so that the heating constant temperature operation is realized, and the rapid nucleic acid amplification is realized in the reagent tube. The control module 9 can also control the LED light source 20 to emit light, the light is guided to the luminous segments 21 horizontally positioned below all the light inlet holes 7 through the light guide strip 19, and the light teeth 22 at the bottom of the luminous segments 21 can enable the luminous segments 21 to emit light upwards and enable the light to be injected into the reagent tube through the light inlet holes 7. At this time, the camera 23 (the model of the camera 23 may be RER-USB30W04 MT) can detect the light at the detection hole 8, and transmit the signal to the control module 9, and the control module 9 analyzes the signal and then transmits the result to the display 3 for displaying the result.
Claims (8)
1. The portable isothermal amplification all-in-one machine comprises a box body (1), wherein a top cover (2) is arranged at the top end of the box body (1); the method is characterized in that: a display screen (3) is connected to one side surface of the box body (1), and a mounting frame (4) is connected to the inside of the box body (1); a placement block (5) is connected to the mounting frame (4), and a constant-temperature heating module is arranged on the placement block (5); the placing block (5) is provided with a plurality of fixing grooves (6) for placing reagent tubes, the placing block (5) is provided with light inlet holes (7) at positions corresponding to the bottoms of the fixing grooves (6), and excitation light sources are arranged at the light inlet holes (7); the position of the placement block (5) corresponding to one side of the bottom of each fixed groove (6) is provided with a detection hole (8), and the detection hole (8) is provided with a detection module; the box body (1) is internally connected with a control module (9), the control module (9) is used for controlling the constant temperature heating module, the excitation light source and the detection module to work, collecting and analyzing signals of the detection module in real time, and transmitting analysis results of the signals to the display screen (3) for display.
2. The portable isothermal amplification all-in-one machine according to claim 1, wherein: the constant temperature heating module comprises a heating plate (10) and a temperature sensor (11) which are connected to the placing block (5); the heating plate (10) and the detection holes (8) are respectively positioned at two sides of the placement block (5).
3. The portable isothermal amplification all-in-one machine according to claim 1, wherein: a heat insulation block (12) is arranged in the top cover (2), and a baffle (13) connected to the top cover (2) is arranged below the heat insulation block (12); the baffle plate (13) is provided with a notch (14) for exposing the heat insulation block (12) at a position right above the plurality of fixing grooves (6).
4. The portable isothermal amplification all-in-one machine according to claim 1, wherein: the top end of the box body (1) is connected with a protective cover plate (15), and a placement hole (16) is formed in the protective cover plate (15) at a position corresponding to each fixing groove (6).
5. The portable isothermal amplification all-in-one machine according to claim 1, wherein: the box body (1) is connected with a power socket (17) and a control button (18) which are electrically connected with the control module (9).
6. The portable isothermal amplification all-in-one machine according to claim 1, wherein: the excitation light source comprises a light guide strip (19) fixed on the mounting frame (4), and one end of the light guide strip (19) is connected with an LED light source (20); the light guide strip (19) is provided with a light emitting section (21), the light emitting section (21) is horizontally arranged below all the light inlet holes (7), and the bottom of the light emitting section (21) is provided with light teeth (22).
7. The portable isothermal amplification all-in-one machine according to claim 1, wherein: the detection module is a camera (23).
8. The portable isothermal amplification all-in-one machine according to claim 1, wherein: one side of the top cover (2) is hinged on the box body (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321760137.8U CN220393747U (en) | 2023-07-06 | 2023-07-06 | Portable isothermal amplification all-in-one machine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321760137.8U CN220393747U (en) | 2023-07-06 | 2023-07-06 | Portable isothermal amplification all-in-one machine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220393747U true CN220393747U (en) | 2024-01-26 |
Family
ID=89603632
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321760137.8U Active CN220393747U (en) | 2023-07-06 | 2023-07-06 | Portable isothermal amplification all-in-one machine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220393747U (en) |
-
2023
- 2023-07-06 CN CN202321760137.8U patent/CN220393747U/en active Active
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