CN215365788U

CN215365788U - Detection assembly for nucleic acid detection and nucleic acid analysis device

Info

Publication number: CN215365788U
Application number: CN202121671232.1U
Authority: CN
Inventors: 周承茂; 邓杏飞; 梁坚诚; 曾汉兵
Original assignee: Guangdong Ardent Biomed Technology Co ltd
Current assignee: Guangdong Ardent Biomed Technology Co ltd
Priority date: 2021-07-21
Filing date: 2021-07-21
Publication date: 2021-12-31
Anticipated expiration: 2031-07-21

Abstract

The present invention relates to the field of nucleic acid detection, and more particularly, to a detection module for nucleic acid detection and a nucleic acid analyzer. A detection assembly for nucleic acid detection, the detection assembly comprising: the mounting box is used for mounting the test paper; a slide rail; the mounting box is connected with the slide rail in a sliding manner; the first detector and the second detector are arranged at intervals along the extending direction of the sliding rail; the mounting box and the sliding rail can slide relatively, so that the mounting box can be located at a first position detected by the first detector and at a second position detected by the second detector. Through the matching of the sliding rail, the first detector and the second detector, the test paper in the mounting box can be detected by different detectors, and different information on the test paper can be read; the integration level of the detection assembly is improved; the floor area of each part in the detection assembly is reduced.

Description

Detection assembly for nucleic acid detection and nucleic acid analysis device

Technical Field

The present invention relates to the field of nucleic acid detection, and more particularly, to a detection module for nucleic acid detection and a nucleic acid analyzer.

Background

Organisms all contain nucleic acids, including deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). In clinical laboratory tests, if a nucleic acid sequence specific for a virus is detected in a patient sample, it is suggested that the patient may be infected with the virus; for example, a nucleic acid detector is used to detect the virus.

However, the existing nucleic acid detector has low integration level and large floor area.

SUMMERY OF THE UTILITY MODEL

An object of the embodiments of the present application is to provide a detection assembly for nucleic acid detection and a nucleic acid analysis apparatus, which aim to solve the problems of low integration and large occupied area of the existing nucleic acid detection apparatus.

The present application provides a detection assembly for nucleic acid detection, the detection assembly comprising:

the mounting box is used for mounting the test paper;

a slide rail; the mounting box is connected with the slide rail in a sliding manner; and

the first detector and the second detector are arranged at intervals along the extending direction of the sliding rail;

the mounting box and the sliding rail can slide relatively, so that the mounting box can be located at a first position detected by the first detector and at a second position detected by the second detector.

Through the matching of the sliding rail, the first detector and the second detector, the test paper in the mounting box can be detected by different detectors, and different information on the test paper can be read; the integration level of the detection assembly is improved; the floor area of each part in the detection assembly is reduced.

In some embodiments of the present application, the detecting assembly further includes a driving member, the driving member is in transmission connection with the mounting box, and the driving member is configured to drive the mounting box to slide relative to the sliding rail, so that the mounting box can be located at the first position and the second position.

In some embodiments of this application, the driving piece includes interconnect's motor, hold-in range, the hold-in range with the mounting box butt, thereby the motor can drive hold-in range motion drives the mounting box is relative the slide rail slides.

In some embodiments of the present application, the first detector is a fluorescence detector;

the second detector is an image collector.

In some embodiments of the present application, the detection assembly for nucleic acid detection further comprises an illumination lamp disposed within the housing, the illumination lamp being configured to illuminate the mounting cartridge at the second position.

The present application also provides a nucleic acid analysis device including:

a housing provided with a test opening;

a test paper incubation component; the test paper incubation component is used for incubating test paper; and

the above-mentioned detecting assembly; the test opening is communicated with the mounting box of the detection assembly;

the test paper incubation assembly and the detection assembly are both arranged in the shell.

The nucleic acid analysis device has all advantages of the detection assembly, in addition, the test paper incubation assembly and the detection assembly are integrated in the shell, the purposes of test paper incubation and result detection can be achieved through the nucleic acid analysis device, and the problems that in the prior art, the occupied area is large and the operation is complex due to the fact that the test paper incubation assembly and the detection assembly need to be independently arranged are solved.

In some embodiments of the present application, the nucleic acid analysis device further comprises a virus inactivation component and a nucleic acid amplification component;

the virus inactivation component and the nucleic acid amplification component are both disposed within the housing.

In some embodiments of the present application, a virus inactivation assembly is configured with a first heating module and a first temperature sensor;

the nucleic acid amplification assembly is configured with a second heating module and a second temperature sensor;

the nucleic acid analysis device further comprises a controller, and the first heating module, the first temperature sensor, the second heating module and the second temperature sensor are all in signal connection with the controller.

In some embodiments of the present application, a strip incubation assembly includes a third heating module and an incubation cavity disposed in the third heating module;

the inner wall of the incubation cavity is provided with an elastic piece for clamping the test paper; the opening of the incubation cavity extends through the housing.

In some embodiments of the present application, the nucleic acid analysis device further comprises an outer cover, the outer cover is rotatably connected to the housing, a display screen is disposed on a side of the outer cover close to the housing, and the display screen is in signal connection with the controller.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained from the drawings without inventive effort.

FIG. 1 is a schematic view showing the structure of a nucleic acid analysis apparatus provided in an embodiment of the present application;

FIG. 2 is a schematic view showing the internal structure of a nucleic acid analysis apparatus according to an embodiment of the present application;

FIG. 3 is a schematic diagram showing a partial structure of a test strip incubation assembly provided in an embodiment of the present application;

fig. 4 shows a schematic structural diagram of a detection assembly provided in an embodiment of the present application.

Icon: 100-a nucleic acid analysis device; 1-a rear cover of a display screen; 2-front cover of display screen; 3-displaying a touch screen; 4-a housing; 6-mounting holes; 7-amplification zone cover; 8-an installation part; 9-opening; 10-an indicator light; 11-test opening; 12-a rubber foot pad; 14-a first heating module, 15-a first temperature sensor; 16-a second heating module; 17-a second temperature sensor; 18-a third heating module; 19-a third temperature sensor; 20-an elastic member; 21-mounting a box; 22-a slide block; 23-a slide rail; 24-a synchronous belt; 25-a motor; 26-a first detector; 27-a second detector; 28-lighting lamp; 29-IC card reading module; 30-a controller; 31-radiator fan.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are some embodiments of the present application, but not all embodiments. The components of the embodiments of the present application, generally described and illustrated in the figures herein, can be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present application, presented in the accompanying drawings, is not intended to limit the scope of the claimed application, but is merely representative of selected embodiments of the application. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In the description of the embodiments of the present application, it should be understood that the terms "inside", "outside", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, or orientations or positional relationships that are conventionally placed when products of the application are used, or orientations or positional relationships that are conventionally understood by those skilled in the art, and are used only for convenience in describing the application and simplifying the description, but do not indicate or imply that the devices or elements that are referred to must have specific orientations, be constructed in specific orientations, and operate, and therefore, should not be construed as limiting the application.

Furthermore, the terms "first," "second," "third," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance.

In the description of the embodiments of the present application, it should also be noted that, unless otherwise explicitly stated or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood in a specific case by those of ordinary skill in the art.

Examples

Fig. 1 shows a schematic configuration diagram of a nucleic acid analysis device 100 provided in an embodiment of the present application, and fig. 2 shows a schematic internal configuration diagram of the nucleic acid analysis device 100 provided in an embodiment of the present application. Referring to fig. 1 and 2, an embodiment of the present application provides a nucleic acid analysis apparatus 100, where the nucleic acid analysis apparatus 100 includes a housing 4, a virus inactivation module, a nucleic acid amplification module, a test strip incubation module, and a detection module.

In the present embodiment, the nucleic acid analysis apparatus 100 is designed mainly based on the isothermal amplification principle; it will be appreciated that in other embodiments of the present application, the nucleic acid amplification module may be designed based on other amplification principles, such as based on PCR amplification principles and the like.

In this embodiment, the viral inactivation module, the nucleic acid amplification module, the strip incubation module, and the detection module are all integrated within the housing 4.

In the present embodiment, the housing 4 is substantially square, and the bottom of the housing 4 is provided with a rubber pad 12 to prevent the housing 4 from sliding relative to the console. In other embodiments of the present application, the shape of the housing 4 is not limited to a square, and may be other shapes such as a cylinder, an ellipse, and the like; accordingly, the rubber foot 12 at the bottom of the housing 4 can be set according to actual requirements.

It is noted that, in some embodiments of the present application, the nucleic acid analysis apparatus 100 may include only a virus inactivation module, a nucleic acid amplification module; in embodiments that include only a viral inactivation module and a nucleic acid amplification module, the amplification of the sample to be detected may be used. Or the nucleic acid analysis device 100 may include only a strip incubation component and a detection component, in this embodiment, may be used to incubate and read the strip.

Referring to fig. 1 and 2, the virus inactivation module is provided with a plurality of mounting holes 6 for receiving the test tubes, and the mounting holes 6 are opened on the housing 4.

The virus inactivation assembly comprises a first heating module 14 and a first temperature sensor 15; the first heating module 14 is connected with the inner wall of the shell 4, and the first temperature sensor 15 is used for detecting the temperature of the first heating module 14; mounting hole 6 sets up on first heating module 14, and first heating module 14 heats for the test tube that is located mounting hole 6, and first temperature sensor 15 is used for detecting the temperature that is heated, and the later stage control temperature of being convenient for contains the sample and heats in the virus inactivation subassembly, makes the virus inactivated.

In the using process, after a sample is inactivated in the virus inactivation assembly, materials such as enzyme, buffer solution and the like are added into the sample and then placed in the nucleic acid amplification assembly for isothermal amplification.

In the present embodiment, the nucleic acid amplification module includes a second heating module 16, a second temperature sensor 17, a mounting portion 8; the mounting part 8 is positioned on the shell 4, and the mounting part 8 is provided with a plurality of mounting holes for mounting sample tubes for amplification; the second heater module 16 heats the mounting portion 8, and the second temperature sensor 17 is connected to the second heater module 16 and detects the temperature of the second heater module 16.

In this embodiment, the mounting portion 8 is further provided with an amplification region cover 7, the amplification region cover 7 is rotatably connected to the housing 4, and the amplification region cover 7 can cover or uncover the mounting portion 8.

The nucleic acid amplification component mainly has the function of providing a relatively stable temperature for the sample tube in the mounting hole, so that materials in the sample tube react under the condition of relatively balanced temperature.

After the reaction of the materials in the sample tube in the nucleic acid amplification component is finished, the materials in the sample tube are dripped onto the test paper, and then the test paper is incubated.

Fig. 3 shows a schematic structural diagram of a part of a test strip incubation assembly provided in an embodiment of the present application.

Referring to fig. 3, 2 and 1, the strip incubation assembly includes a third heating module 18 and a third temperature sensor 19; the third heating module 18 is connected with the third temperature sensor 19, and the third heating module 18 is provided with an incubation cavity for accommodating the test paper.

The third heating module 18 is used for heating the test paper in the incubation cavity to enable materials on the test paper to fully react, and the third temperature sensor 19 is used for detecting the temperature of the third heating module 18.

The opening 9 of the incubation cavity penetrates through the outer shell, the test paper is installed in the incubation cavity from the opening 9, and then the test paper is heated in the incubation cavity by the third heating module 18, so that the materials on the test paper are incubated.

In this application, a plurality of incubation cavities are arranged on the third heating module 18, the incubation cavities are arranged at intervals, and one test paper is installed in one incubation cavity, so that a plurality of test papers can be incubated simultaneously. It should be noted that in other embodiments of the present application, only one incubation cavity may be disposed on the third heating module 18, or the number of incubation cavities may be set according to actual requirements.

In this application, for the convenience of the test paper is relatively fixed at hatching the intracavity, the inner wall of hatching the chamber is provided with elastic component 20, elastic component 20 and the inner wall connection of hatching the chamber, and after the test paper stretched into hatching the intracavity wall, with the test paper locking at hatching the intracavity under elastic component 20's effect, avoid the test paper to remove at hatching the intracavity.

In this embodiment, the elastic member 20 is a spring piece, the spring piece has elastic potential energy in the depth direction of the incubation cavity, and the test paper is fastened by the spring piece after the test paper extends into the incubation cavity; for example, one end of the test paper is supported by the end of the spring piece, and the other end is supported by the inner wall of the shell 4; the test paper is locked.

In other embodiments of the present application, the elastic member is not limited to the spring plate, and may be a member having elastic potential energy, such as an elastic rubber strip or a spring.

It should be noted that, in other embodiments of the present application, the elastic member is not necessary, and the third heating module 18 may not be provided with the elastic member.

The test paper is heated by the third heating module 18 in the incubation cavity, so that the material on the test paper is incubated, and after the incubation is finished, the test paper is placed in the detection assembly for detection.

Referring to fig. 4 and fig. 1, the detecting assembly is mainly used for detecting the incubated material on the test paper.

In the application, the detection assembly comprises a mounting box 21, a slide rail 23, a first detector 26 and a second detector 27, wherein the mounting box 21 is connected with the slide rail 23 in a sliding manner; the mounting box 21 is used for mounting test paper; the first detector 26 and the second detector 27 are disposed at intervals along the extending direction of the slide rail 23.

The test opening 11 of the mounting box 21 is located on the housing 4, and the test paper is mounted in the mounting box 21 through the test opening 11 of the housing 4.

After the mounting box 21 slides relative to the slide rail 23, the mounting box 21 can be located at a first position detected by the first detector 26 and can be located at a second position detected by the second detector 27.

In other words, after the mounting box 21 slides relative to the slide rail 23, the relative position between the mounting box 21 and the slide rail 23 changes, and the first detector 26 and the second detector 27 are both located in the extending direction of the slide rail 23, so that the test paper in the mounting box 21 can be located in the detection area of the first detector 26 and the detection area of the second detector 27.

Further, in the embodiment of the present application, the mounting box 21 is provided with the sliding block 22, the sliding block 22 is located on a side wall of the mounting box 21, and the relative sliding between the sliding block 22 and the sliding rail 23 is realized through the relative sliding between the sliding block 22 and the sliding rail 23. It will be appreciated that in other embodiments of the present application, the slider 22 may be disposed at the bottom of the mounting box 21.

In this embodiment, the detecting assembly further includes a driving member for driving the mounting box 21 to slide relative to the slide rail 23.

In this embodiment, the driving member includes a motor 25 and a timing belt 24, the timing belt 24 abuts against the mounting box 21, and the motor 25 can drive the timing belt 24 to move so as to drive the mounting box 21 to slide relative to the slide rail 23. The motor 25 is connected with the synchronous belt 24, the motor 25 drives the synchronous belt 24 to move, and the moving direction of the synchronous belt 24 is the same as the extending direction of the sliding rail 23.

The slider 22 of the mounting box 21 abuts against the synchronous belt 24, and after the synchronous belt 24 moves, the slider 22 and the synchronous belt 24 move together, so that the mounting box 21 is driven to move along the moving direction of the synchronous belt 24.

As described above, the first detector 26 and the second detector 27 can detect the test paper in the mounting cassette 21 during the movement of the mounting cassette 21 in the movement direction of the timing belt 24.

For example, in the embodiment of the present application, the motor 25 is a stepping motor, and it should be noted that in other embodiments of the present application, the motor may be another type of motor. In addition, in other embodiments of the present application, the driving member may be other components, for example, the driving member includes a motor and a rack and pinion, the motor outputs a rotary motion, and the rotary motion is converted into a linear motion by the conversion of the rack and pinion.

Alternatively, in other embodiments of the present application, no driving member may be provided, for example, a manual force is used to push the mounting box 21, so that the mounting box 21 moves along the sliding rail 23, and the mounting box 21 can reach the areas detectable by the first detector 26 and the second detector 27.

In this embodiment, in order to cool down the motor 25, the detection assembly is further provided with a cooling fan 31 for cooling down the motor 25, the cooling fan 31 is installed on the housing 4, and the heat dissipated by the motor 25 is dissipated to the outside of the housing 4 through the effect of the circulating air.

In the present embodiment, the first detector 26 is a fluorescence detector; the first detector 26 is used for detecting whether the material on the test paper fluoresces and outputting corresponding fluorescence band information. The second detector 27 is an image collector, for example, the second detector 27 is a camera or a video camera, and the second detector 27 is configured to obtain information on the test strip and output corresponding information.

It should be noted that, in other embodiments of the present application, the first detector 26 and the second detector 27 may be other detection devices, and are set according to the performance and information that need to be detected on the test strip.

Further, in order to preferably collect information on the test paper, the detection assembly is further provided with an illumination lamp 28, and the illumination lamp 28 is used for illuminating the mounting box 21 located at the second position. In other words, the illuminating lamp 28 is mainly used to provide light to the area that can be irradiated by the second detector 27.

It is understood that in other embodiments of the present application, the illumination lamp 28 may not be provided, for example, a part of the housing 4 may be provided as a transparent window, and natural light may be used to facilitate the information of the mounting box 21 at the second position.

Referring to fig. 4 again, in the present embodiment, the nucleic acid analyzer 100 further includes a controller 30, and the controller 30 is mainly used for providing signal control for the heating module and the like inside the nucleic acid analyzer 100.

For example, in the present embodiment, the first heater module 14, the second heater module 16, the third heater module 18, the third temperature sensor 19, the first temperature sensor 15, and the second temperature sensor 17 are all in signal connection with the controller 30.

For example, the third temperature sensor 19, the first temperature sensor 15, and the second temperature sensor 17 transmit the detected temperature signals to the controller 30, and the controller 30 compares the signals with a target temperature, and controls the first heater module 14, the second heater module 16, and the third heater module 18 to keep their temperatures within a predetermined temperature range.

Further, the controller 30 may be configured to control whether to turn on the first heating module 14, the second heating module 16, or the third heating module 18 according to the current use state of the nucleic acid analysis device 100. For example, when the sample is in the strip incubation period, the controller 30 may control the first heating module 14, the second heating module 16 to turn off, and control the temperature of the third heating module 18 to reach a preset value.

Further, the controller 30 may be in signal connection with the first detector 26, the second detector 27, the motor 25, and the illumination lamp 28.

The first detector 26 and the second detector 27 convert the detected information into electrical signals respectively and transmit the electrical signals to the controller 30, and the controller 30 transmits the information to the receiving end.

The controller 30 can control whether the motor 25 and the illuminating lamp 28 are turned on or not; further, the controller 30 may also be configured to control the motor 25 to rotate forward or the motor 25 to rotate backward, etc. according to actual requirements.

For example, the controller 30 may be a single chip microcomputer.

Referring to fig. 1 again, in the present embodiment, the nucleic acid analysis device 100 further includes a display screen rear cover 1, a display screen front cover 2, and a display touch screen 3; lid 1 and display screen protecgulum 2 fixed connection behind the display screen, show that touch-sensitive screen 3 sets up on display screen protecgulum 2, and lid 1 and display screen protecgulum 2 all rotate with shell 4 and are connected behind the display screen.

The first detector 26 and the second detector 27 are both in signal connection with the controller 30, and the controller 30 is in signal connection with the display touch screen 3.

In the present embodiment, the controller 30 transmits information detected by the first and second detectors 26 and 27 to the display touch screen 3; information can be read on the display touch screen 3. The touch screen 3 may also be used to input commands, for example, preset temperatures of the first, second, and

third heating modules

14, 16, and 18 are input, and the input commands are transmitted to the controller 30, and the controller 30 controls the first, second, and

third heating modules

14, 16, and 18 to actually obtain the required heating temperatures according to the commands.

In this embodiment, an IC card reading module 29 is further disposed in the housing 4, the IC card reading module 29 is in signal connection with a controller 30, and the controller 30 can read information in the IC card reading module 29.

In the embodiment, the housing 4 is provided with the indicator lamp 10, the indicator lamp 10 is in signal connection with the controller 30, and when the information on the test paper is being detected by the first detector 26 and the second detector 27, the controller 30 controls the indicator lamp 10 to emit light; when the information on the test strip is detected by the first detector 26 and the second detector 27, the controller 30 controls the indicator lamp 10 not to emit light.

It should be noted that, in other embodiments of the present application, the display rear cover 1, the display front cover 2, and the display touch screen 3 are not essential, and may be independent of the nucleic acid analysis device 100; the display touch screen 3 may be provided to other terminals, for example.

The detection assembly provided by the embodiment of the application has at least the following advantages:

through the matching of the slide rail, the first detector 26 and the second detector 27, the test paper in the mounting box 21 can be detected by different detectors, and different information on the test paper can be read; the integration level of the detection assembly is improved; the floor area of each part in the detection assembly is reduced.

The nucleic acid analysis device 100 according to the embodiment of the present application has at least the following advantages:

the nucleic acid analysis device 100 has all the advantages of the detection assembly, and in addition, the test paper incubation assembly and the detection assembly are integrated in the shell 4, so that the purposes of test paper incubation and result detection can be realized through the nucleic acid analysis device 100, and the problems of large occupied area and complex operation caused by the need of separately arranging the test paper incubation assembly and the detection assembly in the prior art are solved.

Further, in some embodiments, the nucleic acid analysis device 100 also has a virus inactivation module, a nucleic acid amplification module; the steps of virus inactivation, nucleic acid amplification, strip incubation and result detection can be completed in the nucleic acid analysis device 100, and the integration level of the nucleic acid analysis device 100 is further improved.

The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A detection assembly for nucleic acid detection, the detection assembly comprising:

the mounting box is used for mounting the test paper;

2. The detection assembly for nucleic acid detection according to claim 1, further comprising a driving member drivingly coupled to the mounting block, the driving member configured to drive the mounting block to slide relative to the slide rail so that the mounting block can be located at the first position and the second position.

3. The detecting assembly for detecting nucleic acid according to claim 2, wherein the driving member includes a motor and a synchronous belt connected to each other, the synchronous belt abuts against the mounting box, and the motor can drive the synchronous belt to move so as to drive the mounting box to slide relative to the slide rail.

4. The detection assembly for nucleic acid detection according to claim 1, wherein the first detector is a fluorescence detector;

the second detector is an image collector.

5. The detection assembly for nucleic acid detection according to claim 4, further comprising an illumination lamp for illuminating the mounting cartridge at the second position.

6. A nucleic acid analysis device, comprising:

a housing provided with a test opening;

the detection assembly of any one of claims 1-5; the test opening is communicated with the mounting box of the detection assembly;

7. The nucleic acid analysis device according to claim 6, further comprising a virus inactivation module and a nucleic acid amplification module;

8. The nucleic acid analysis device according to claim 7, wherein the virus inactivation module is provided with a first heating module and a first temperature sensor;

9. The nucleic acid analysis device of claim 6, wherein the strip incubation assembly comprises a third heating module and an incubation cavity disposed in the third heating module;

10. The nucleic acid analysis device of claim 8, further comprising an outer cover rotatably coupled to the housing, wherein a display screen is disposed on a side of the outer cover adjacent to the housing, and the display screen is in signal communication with the controller.