CN221028439U

CN221028439U - Portable nucleic acid detecting device

Info

Publication number: CN221028439U
Application number: CN202322591645.4U
Authority: CN
Inventors: 耿静; 周颖; 马勃; 游杰颖; 陈彦佑; 吴彦宏; 黄柏尧; 萧永庆; 卢琛; 张微石
Original assignee: Delta Electronics International Singapore Pte Ltd
Current assignee: Delta Electronics International Singapore Pte Ltd
Priority date: 2022-09-30
Filing date: 2023-09-22
Publication date: 2024-05-28
Anticipated expiration: 2033-09-22
Also published as: JP2024052616A; TWM654791U; CN117801935A; TW202415451A

Abstract

The utility model discloses a portable nucleic acid detection device. The portable nucleic acid detecting device comprises a base, a circuit board, a heating module, an optical module, a fluid cassette and a shell. The base is provided with an accommodating space, and the circuit board is arranged in the accommodating space of the base. The heating module and the optical module are electrically connected to the circuit board. The fluid cassette is correspondingly arranged on the circuit board and the heating module. The shell is correspondingly covered on the base and seals the accommodating space. After the sample is placed in the fluid cassette, the sample is heated by a heating component of the heating module, the temperature is maintained at a set temperature, so that the sample is subjected to nucleic acid amplification, the sample is irradiated by a light emitter of the optical module, the irradiated spectrum is received by the light sensor, detection comparison is performed, and a detection result is displayed.

Description

Portable nucleic acid detecting device

Technical Field

The present utility model relates to a portable nucleic acid detecting apparatus, and more particularly, to a portable nucleic acid detecting apparatus capable of rapidly amplifying and detecting multiple nucleic acids.

Background

In order to obtain a large amount of specific DNA fragments according to different requirements, the development has been vigorous in recent years, and scientists have been striving to find an efficient way to meet the goal, and polymerase chain reaction (Polymerase chain reaction, PCR) is one of the most economical and rapid technologies, and can obtain billions of copies of specific DNA fragments in a short time. PCR techniques can be applied in a variety of fields, such as selective DNA isolation for genetic identification, forensic analysis for analysis of ancient DNA in archaeology, medical applications for gene detection and tissue type, rapid and accurate diagnosis of infectious diseases in hospitals and research institutions, environmental hazard inspection for food safety, and gene fingerprinting for investigation of criminals, etc. The PCR technique can detect amplified DNA fragments by fluorescent molecules by using only a small amount of DNA sample extracted from blood or tissue and adding a fluorescent dye to a nucleic acid solution.

Infectious diseases are caused by pathogens, including bacteria, viruses and parasites. To effectively prevent an outbreak of an infectious disease, a rapid, low cost, accurate on-site diagnosis is needed. Therefore, there is a real-time urgent need for low-cost, rapid point-of-care testing (POCT) technology that can be developed for infectious pathogens outside of hospitals and laboratories.

The current state of the art rapid diagnostic kits do not provide low cost multiplex assays. For example, cue Health, lucira, healthPod currently on the market are both single disease diagnoses, which provide only a single target test with different sensitivities, but not multiple tests. As for other companies that may provide multiple tests, more expensive techniques and complex fluid control systems are employed, resulting in higher machine and test costs, the pricing and requirements of such complex mechanical valve systems are burdensome to the general public. For example, if the general public wants to perform multiple PCR assays, the samples need to be sent to a large laboratory to perform multiple PCR assays through multiple complex mechanical valve systems, which not only requires expensive testing equipment and complex testing procedures, but also consumes a lot of labor and time costs.

Therefore, in order to improve the drawbacks of the known complex multiplex PCR detection devices and detection procedures, a portable nucleic acid detection device that is portable, usable anytime and anywhere, i.e. cost-effective, should be developed to provide highly sensitive multiplex diagnostic medical devices to the public, and should be simple, easy to handle and carry, so that the user can understand and use with minimal training, and can quickly learn the detection results.

Disclosure of utility model

The utility model aims to provide a portable nucleic acid detection device, which is formed by arranging a fluid cartridge, a heating module, an optical module and a circuit board in a single device formed by assembling a base and a shell, so that the portable nucleic acid detection device has the advantages of compact structure, smaller volume and lighter weight, is convenient for a user to carry, and can perform multiple nucleic acid amplification and detection at any time and any place, thereby saving detection time and cost.

According to one aspect of the present utility model, there is provided a portable nucleic acid detecting apparatus comprising: a base, a circuit board, a heating module, an optical module, a fluid cartridge, and a housing. The base is provided with an accommodating space, and the circuit board is arranged in the accommodating space of the base. The heating module is provided with at least one heating component and is electrically connected to the circuit board. The optical module is provided with at least one light emitter and at least one light sensor and is electrically connected to the circuit board. The fluid cassette is correspondingly arranged on the circuit board and the heating module. The shell is correspondingly covered on the base and seals the accommodating space. After a sample is placed in the fluid cassette, the sample is heated by at least one heating component of the heating module and kept at a constant temperature at a set temperature, so that the sample is subjected to nucleic acid amplification, the sample is irradiated by at least one light emitter of the optical module, the irradiated spectrum is received by at least one light sensor, detection comparison is performed, and a detection result is displayed.

According to one embodiment of the present utility model, the portable nucleic acid detecting apparatus further comprises a button and a display.

According to one embodiment of the present utility model, the key and the display portion are disposed on the upper surface of the housing.

According to one embodiment of the present utility model, the upper surface of the housing further has an opening, and the opening corresponds to the detection cartridge body of the fluid cartridge.

According to one embodiment of the present utility model, the portable nucleic acid detecting apparatus further has a cover, and is correspondingly sealed on the opening of the housing.

According to one embodiment of the present utility model, the key and the display portion are disposed on the base.

According to one embodiment of the utility model, the circuit board is formed by a main circuit board and an additional circuit board, and the additional circuit board is arranged perpendicular to the main circuit board.

According to one embodiment of the utility model, a plurality of indicator lights are provided on the additional circuit board.

According to one embodiment of the present utility model, the portable nucleic acid detecting apparatus further comprises a positioning seat correspondingly disposed between the circuit board and the fluid cartridge, and a recess is formed on a side surface of the positioning seat for corresponding engagement with the additional circuit board to assist positioning.

According to another aspect of the present utility model, there is provided a portable nucleic acid detecting apparatus comprising: a base, a circuit board, a heating module, an optical module, a fluid cartridge, a housing, and a cover. The base is provided with an accommodating space, and the circuit board is arranged in the accommodating space of the base. The heating module is provided with at least one heating component and is electrically connected to the circuit board. The optical module is provided with at least one light emitter and at least one light sensor and is electrically connected to the circuit board. A fluid cassette is correspondingly arranged on the circuit board and the heating module. The shell is correspondingly covered on the base and is provided with an opening, and the opening corresponds to the detection box body of the fluid cartridge. The cover is correspondingly arranged on the opening of the shell so as to isolate the fluid cassette from the external environment. After the sample is placed in the fluid cassette, the sample is heated by at least one heating component of the heating module and kept at a constant temperature at a set temperature, so that the sample is subjected to nucleic acid amplification, the sample is irradiated by at least one light emitter of the optical module, the irradiated spectrum is received by at least one light sensor, detection and comparison are performed, and the detection result is displayed.

The utility model has the beneficial effects that the utility model provides the portable nucleic acid detection device, which enables the sample in the fluid cassette to carry out isothermal nucleic acid amplification by tightly matching the fluid cassette with the circuit board and the heating module thereon, and carries out nucleic acid detection by the optical module correspondingly arranged on the circuit board. Therefore, the fluid cassette, the heating module, the optical module and the circuit board are arranged in a single device formed by assembling the base and the shell, so that the portable nucleic acid detection device has the advantages of compact structure, smaller volume and lighter weight, is convenient for a user to carry, can amplify and detect multiple nucleic acids anytime and anywhere, does not need to send a sample to a large laboratory for nucleic acid detection, and can greatly save detection time and cost.

Drawings

FIG. 1 is a schematic view of a portable nucleic acid detecting apparatus according to a first embodiment of the present utility model.

FIG. 2 is a schematic diagram of an exploded structure of the portable nucleic acid detecting apparatus shown in FIG. 1.

FIG. 3 is a schematic diagram showing a partially assembled structure of the portable nucleic acid detecting apparatus shown in FIG. 1.

FIG. 4 is a schematic diagram of a control system of the portable nucleic acid detecting apparatus shown in FIG. 1.

FIG. 5 is a schematic diagram showing the appearance of a portable nucleic acid detecting apparatus according to a second preferred embodiment of the present utility model.

FIG. 6 is a schematic diagram of an exploded structure of the portable nucleic acid detecting apparatus shown in FIG. 5.

FIG. 7 is a schematic diagram showing a partially assembled structure of the portable nucleic acid detecting apparatus shown in FIG. 5.

The reference numerals are as follows:

1. 3: portable nucleic acid detecting device

10. 30: Base seat

100. 300: Accommodating space

101: Bottom plate

102: Side wall

103. 311: Display unit

104. 312: Key-press

11. 31: Shell body

12. 32: Circuit board

120: Micro control unit

121: Main circuit board

122: Additional circuit board

13: Heating module

131: Heating assembly

132: Thermal sensor

14: Optical module

141: Light emitter

142: Light sensor

143: Optical driver

144. 321: Indicating lamp

15: Power supply module

151: Battery management system

152: Battery cell

16: Regulator

17: USB port

18: Crystal oscillator

19: Positioning seat

190: Circular opening

191: Concave part

2. 4: Fluid cassette

20: Body

21. 41: Detection box body

310: An opening

313: Upper surface of

33: Cover cap

Detailed Description

Some exemplary embodiments embodying features and advantages of the present utility model will be described in detail in the following description. It will be understood that the utility model is capable of modification in various other forms without departing from the scope of the utility model, and that the description and illustrations herein are intended to be by way of illustration only and not to be construed as limiting the utility model.

Please refer to fig. 1, 2, 3 and 4. FIG. 1 is a schematic view of a portable nucleic acid detecting apparatus according to a first embodiment of the present utility model. FIG. 2 is a schematic diagram of an exploded structure of the portable nucleic acid detecting apparatus shown in FIG. 1. FIG. 3 is a schematic diagram showing a partially assembled structure of the portable nucleic acid detecting apparatus shown in FIG. 1. FIG. 4 is a schematic diagram of a control system of the portable nucleic acid detecting apparatus shown in FIG. 1. As shown in the drawing, the portable nucleic acid detecting apparatus 1 of the present utility model comprises a base 10, a housing 11, a circuit board 12, a heating module 13 (shown in FIG. 4), an optical module 14 (shown in FIG. 4), and a fluid cartridge 2. The base 10 has an accommodating space 100 therein, and the housing 11 is correspondingly covered on the base 10 and seals the accommodating space 100 of the base 10. The circuit board 12 is disposed in the accommodating space 100 of the base 10. As shown in fig. 4, the heating module 13 has at least one heating element 131 and is electrically connected to the circuit board 12; the optical module 14 has at least one light emitter 141 and at least one light sensor 142, and is electrically connected to the circuit board 12. In some embodiments, the heating module 13 and the optical module 14 are disposed on the circuit board 12, but not limited thereto. The fluid cassette 2 is disposed in the accommodating space 100 of the base 10 and is disposed on the heating module 13 and the circuit board 12 correspondingly. After a sample is placed in the fluid cassette 2, the sample is heated by at least one heating element 131 of the heating module 13 and maintained at a constant temperature to amplify the nucleic acid, and then the sample is irradiated by at least one light emitter 141 of the optical module 14 and the irradiated spectrum is received by at least one light sensor 142 to perform detection and comparison, and the detection result is displayed.

Please refer to fig. 1, fig. 2 and fig. 3. As shown in the drawing, the base 10 of the present embodiment is a box structure formed by a bottom plate 101 and a side wall 102 surrounding the bottom plate 101, and has a receiving space 100 therein for correspondingly receiving the circuit board 12, the heating module 13, the optical module 14 and the fluid cartridge 2. As shown in fig. 1, when the housing 11 is covered on the base 10, it can completely seal the accommodating space 100, and the components such as the circuit board 12, the heating module 13, the optical module 14, and the fluid cartridge 2 disposed therein are effectively isolated from the external environment, so as to achieve the effect of protection and isolation. The outer surface of the side wall 102 of the base 10 is provided with a key 104 and a display part 103, the key 104 and the display part 103 are electrically connected to the circuit board 12, the key 104 is used for starting or closing the portable nucleic acid detecting device 1, and the display part 103 is used for displaying the detection result. In this embodiment, the display unit 103 may be, but not limited to, a plurality of display windows for displaying the detected light signal, so that the user can quickly and easily understand the detection result. However, in other embodiments, the display portion 103 may be, but is not limited to, a liquid crystal display for viewing the detailed detection result, and the display portion 103 is mainly used for displaying the detection result, and the type and the shape thereof may be changed according to the actual implementation situation, which is not limited to the foregoing embodiment.

As shown in fig. 2 and 3, in the present embodiment, the circuit board 12 is disposed in the accommodating space 100 of the base 10 and disposed on the bottom plate 101. In addition, in the present embodiment, the portable nucleic acid detecting apparatus 1 further includes a positioning seat 19, where the positioning seat 19 is disposed between the circuit board 12 and the fluid cassette 2, and has a circular opening 190, and the appearance and the size of the circular opening 190 correspond to the detecting box 21 of the fluid cassette 2. As shown in fig. 3, the positioning seat 19 is correspondingly disposed on the circuit board 12, so that the detection cartridge 21 of the fluid cartridge 2 is correspondingly disposed on the circuit board 12 through the circular opening 190, thereby assisting in positioning the detection cartridge 21 on the heating module 13 and the detection module 14 of the circuit board 12. In addition, in the present embodiment, the circuit board 12 may be, but is not limited to, a main circuit board 121 and an additional circuit board 122, wherein the additional circuit board 122 has a smaller area than the main circuit board 121 and is disposed perpendicular to the main circuit board 121, but the size, the disposition and the disposition of the additional circuit board 122 are not limited thereto. In some embodiments, the additional circuit board 122 may be, but is not limited to, one or more light guide plates. In still other embodiments, the circuit board 12 may be a single circuit board, and is not limited to the implementation with two circuit boards. As shown in fig. 2, the light emitters 141 and the light sensors 142 of the optical module 14 of the present embodiment are all connected and disposed on the main circuit board 121, and the main circuit board 121 is mainly used for accommodating necessary related chips and electronic components. In addition, an indicator light 144 is disposed on the additional circuit board 122, and the indicator light 144 is configured to receive a signal sent by the micro control unit 120 (as shown in fig. 4) of the circuit board 12, so as to correspondingly display the detection result. In some embodiments, the additional circuit board 122 is used to display the detection result and/or to correspondingly activate the detection through a switch (or button) (not shown), and the switch (or button) is electrically connected to the key 104 of the base 10. In the present embodiment, when the circuit board 12 is correspondingly disposed in the accommodating space 100 of the base 10, the additional circuit board 122 is disposed corresponding to the front side wall 102 of the base 10. That is, the position of the indicator light 144 on the additional circuit board 122 corresponds to the display window of the display portion 103 on the side wall 102. Therefore, when the signal received by the indicator light 144 is correspondingly lightened, a user can quickly obtain the detection result through the lightened display window of the display part 103 on the base 10. In addition, in the present embodiment, the positioning seat 19 further has a recess 191 on a side surface thereof corresponding to the additional circuit board 122 for accommodating the additional circuit board 122. When the circuit board 12 and the positioning seat 19 are disposed in the accommodating space 100 of the base 10, the positioning seat 19 can be more firmly engaged with the circuit board 12 by the corresponding arrangement of the recess 191 on the side surface of the positioning seat 19 and the additional circuit board 122.

Please refer to fig. 4. FIG. 4 is a schematic diagram of a control system of the portable nucleic acid detecting apparatus shown in FIG. 1. In the present embodiment, as shown in fig. 4, the control system of the circuit board 12 is shown, wherein the micro control unit 120 is disposed on the circuit board 12 and electrically connected to each functional module (the heating module 13, the optical module 14, the power module 15 and the crystal oscillator 18) to receive the related information transmitted by the plurality of functional modules, perform data calculation, analysis and processing, and further control the plurality of functional modules to operate. As shown, the heating module 13 includes a heating element 131 and a thermal sensor 132, wherein the thermal sensor 132 is used for sensing temperature and transmitting the sensed temperature information to the micro-controller unit 102 for precise temperature control. In some embodiments, the heating element 131 may be, but is not limited to, a heating chip disposed on the circuit board 12, which may be heated by joule heating or peltier effect. The thermal sensor 132 and the heating element 131 in the heating module 13 are used for continuously sensing the temperature and precisely controlling the temperature to maintain the temperature at a constant temperature state with a specific temperature, so that the sample can be subjected to multiple reactions such as isothermal nucleic acid amplification, polymerase Chain Reaction (PCR) and the like.

In addition, as shown, the optical module 14 is composed of a plurality of optical components for detecting the optical signal generated by the nucleic acid amplification. The optical module 14 includes components such as a light emitter 141, a light sensor 142, and a light driver 143. In this embodiment, the light emitters 141 may be, but are not limited to, LEDs, and each light emitter 141 is electrically connected to a corresponding light driver 143 for driving the light emitter 141 to emit light beams. Of course, each light emitter 141 is also disposed corresponding to one light sensor 142, and when the light emitter 141 is driven by the light driver 143 to emit light beams, the corresponding light sensor 142 can receive the optical signals after the sample is irradiated, and then transmit the optical signals to the corresponding indicator light 144 to display the detection result. In some embodiments, the detection method may include, but is not limited to, colorimetric detection, fluorescent detection …, and the like.

In the present embodiment, the portable nucleic acid detecting apparatus 1 can be used as a stand-alone device or connected to an external electronic device (e.g., a computer or a mobile phone) via Bluetooth or a cable. In some embodiments, the micro control unit 120 may support bluetooth functions, USB data transmission, and the like, but is not limited thereto. The detection result of the optical signal is transmitted to the micro-control unit 120, so that calculation and analysis can be performed according to the detection result, and a plurality of analysis data and test results are stored in the micro-controller unit 120. In other embodiments, the analysis data and the test results may be transmitted to an external electronic device (e.g., a computer or a mobile phone) via a wired or wireless connection. For example, the micro controller unit 120 is electrically connected to the USB port 17 through the regulator 16 to perform USB data transmission, but not limited to this. In other embodiments, the USB port 17 may be, but is not limited to, a USB Type C port. In this embodiment, the portable nucleic acid detecting apparatus 1 further includes a power module 15 for providing power, the power module 15 includes a battery management system 151 and at least one battery 152, and the battery management system 151 regulates and manages the at least one battery 152 to output the required power. In some embodiments, the battery 152 is a rechargeable battery, but is not limited thereto. In other embodiments, the portable nucleic acid detecting apparatus 1 can be further connected to an electronic calculator, a charging device or an ac mains supply via a cable (not shown), but is not limited thereto.

Please refer to fig. 2, fig. 3 and fig. 4. As shown in the drawing, in the present embodiment, the method for detecting the portable nucleic acid detecting apparatus 1 mainly includes the following steps: firstly, a user firstly obtains a swab sample, then, the swab sample is placed in the body 20 of the fluid cartridge 2, and the body 20 is correspondingly arranged in the detection box 21, so that the swab sample can correspondingly flow into the detection box 21. In the present embodiment, the detection cartridge 21 has a plurality of detection chambers (not shown), and the detection cartridge 21 is correspondingly disposed on the heating module 13. Thereafter, when the user subsequently covers the housing 11 on the base 10, the sample to be tested is isolated from the outside. And, when the user presses the key 104 on the base 10, the key 104 is electrically connected with a switch (or a button) on the circuit board 12, so as to start the portable nucleic acid detection device 1, and the micro control unit 120 sends a control signal to start heating the heating module 13, so that the sample is subjected to reactions such as isothermal nucleic acid amplification, polymerase Chain Reaction (PCR) and the like; after a specific reaction time (for example, 20 minutes or 30 minutes), the optical module 14 can detect the optical signal generated after the nucleic acid amplification, and the indicator light 144 on the circuit board 12 displays the detection result, so that the user can directly and quickly understand the detection result through the display part 103 on the base 10, and further automatically complete the nucleic acid detection.

In some embodiments, the portable nucleic acid detecting apparatus 1 can also be configured to automatically start the nucleic acid detecting process after the user covers the housing 11 on the base 10, in other words, it is not necessary to press the key 104 by the user to start the portable nucleic acid detecting apparatus 1, but not limited thereto. In the embodiment, the housing 11 can ensure the close contact between the fluid cartridge 2 and the circuit board 12 and the heating module 13 thereon, so that heat can be effectively transferred to the fluid cartridge 2 above the circuit board 12, and the heating module 13 can be directly activated in the close-fitting state of the housing 11 and the base 10 to perform isothermal nucleic acid amplification and subsequent nucleic acid detection procedures. In addition, in other embodiments, in order to ensure the close contact between the fluid cartridge 2 and the circuit board 12 and the heating module 13 thereon, the circuit board 12 may also be designed as a floating circuit board, for example, the circuit board 12 may be supported by an elastic component such as a spring, so that the circuit board 12 has a spatial degree of freedom capable of being displaced up and down, so that the initial position when the circuit board is not under pressure is higher, but if the fluid cartridge 2 is pressed downward according to the housing 11, the circuit board 12 is synchronously pushed downward, and the body 20 of the fluid cartridge 2 is engaged with the fluid cartridge 2, so as to contact the heating module 13 disposed thereunder. Of course, in this embodiment, a locking mechanism (not shown) may also be provided, such as: the concave-convex corresponds to the clamping mechanism so as to maintain the fluid cassette 2 and the circuit board 12 and the heating module 13 thereon in a stable and close contact state, thereby effectively performing heat transfer and subsequent multiplex PCR amplification and detection procedures. In addition, in other embodiments, the portable nucleic acid detecting apparatus 1 may also perform the nucleic acid detecting process without the housing 11, which may be changed according to the actual implementation situation, and is not limited to the above embodiments.

Please refer to fig. 5, 6 and 7. FIG. 5 is a schematic diagram showing the appearance of a portable nucleic acid detecting apparatus according to a second preferred embodiment of the present utility model. FIG. 6 is a schematic diagram of an exploded structure of the portable nucleic acid detecting apparatus shown in FIG. 5. FIG. 7 is a schematic diagram showing a partially assembled structure of the portable nucleic acid detecting apparatus shown in FIG. 5. As shown in the drawing, the portable nucleic acid detecting apparatus 3 of the present utility model comprises a base 30, a housing 31, a circuit board 32, a heating module (not shown), an optical module (not shown) and a fluid cartridge 4. The base 30 also has a receiving space 300 therein, and the housing 31 is correspondingly covered on the base 30 and seals the receiving space 300 of the base 30. The circuit board 32 is disposed in the accommodating space 300 of the base 30. In the present embodiment, the heating module and the optical module are disposed on the circuit board 32 in a connecting manner, but not limited thereto. The fluid cassette 4 is disposed in the accommodating space 300 of the base 30, and the corresponding arrangement is on the circuit board 32 and the heating module thereof. In the present embodiment, the heating module, the optical module, and the control system and related components on the circuit board 32 are the same as those in the previous embodiment, so that the description thereof is omitted. After a sample is placed in the fluid cassette 4, the sample is heated by at least one heating component of the heating module and kept at a constant temperature at a set temperature, so that the sample is subjected to nucleic acid amplification to form an object to be detected, and then the sample is irradiated by at least one light emitter of the optical module and irradiated by at least one light emitter, and the irradiated spectrum is received by at least one light sensor to perform detection comparison and display a detection result. However, in this embodiment, the design of the appearance of the portable nucleic acid detecting apparatus 3 is slightly different from that of the previous embodiment. That is, the housing 31 of the present embodiment has an opening 310, and the opening 310 is disposed on the upper surface 313 of the housing 31, and its appearance and size correspond to those of the detection cartridge 41 of the fluid cartridge 4. Therefore, after the circuit board 32 and the detection cartridge 41 are correspondingly disposed in the accommodating space 300 of the base 30, the housing 31 is sealed on the base 30, and at this time, as shown in fig. 7, the detection cartridge 41 correspondingly disposed on the circuit board 32 can be seen through the opening 310 of the housing 31. In addition, in the present embodiment, the opening 310 provided on the housing 31 is helpful for assisting the positioning of the detection cartridge 41 of the fluid cartridge 4 in the accommodating space 300 of the base 30, which is equivalent to the function of the housing 31 to position the detection cartridge 41, so that the components of the portable nucleic acid detecting device 3 are simplified without using an additional positioning seat in the present embodiment. In addition, in the present embodiment, the upper surface 313 of the housing 31 is further provided with a button 312 and a display portion 311, so that the user can press the button 312 to start/close the portable nucleic acid detecting apparatus 3, and directly observe the nucleic acid detecting result through the display window of the display portion 311. In addition, in the present embodiment, the portable nucleic acid detecting apparatus 3 further includes another cover 33, which is correspondingly sealed on the opening 310 of the housing 31, so as to isolate the fluid cartridge 4 from the external environment.

In this embodiment, the method for detecting the portable nucleic acid detecting apparatus 3 is similar to the previous embodiment, and mainly includes the following steps: first, when the user covers the housing 31 on the base 30, the detection cartridge 41 disposed on the circuit board 32 can be seen through the opening 310 of the housing 31. Next, the user first obtains a swab sample and places the swab sample in the body 40 of the fluid cartridge 4; thereafter, as shown in fig. 7, the body 40 is disposed on the detection cartridge 41 through the opening 310 of the housing 31, so that the swab sample can flow into the detection cartridge 41. Similarly, the detection cartridge 41 also has a plurality of detection chambers (not shown) for multiple nucleic acid amplification and detection, so that the swab sample can detect multiple targets or diseases simultaneously in a single detection cartridge 41. In the present embodiment, the detecting box 41 is also disposed on the circuit board 32 and the heating module thereof. Therefore, when the user covers the cover 33 on the opening 310 of the housing 31, the sample to be tested is isolated from the outside, and the fluid cartridge 4 is ensured to be in close contact with the circuit board 32 and the heating module thereon. Therefore, when the user presses the button 312 on the housing 31, the button 312 is electrically connected with a switch (or button) on the circuit board 32, so as to start the portable nucleic acid detecting device 3, so that the heating module starts heating, and the heat can be effectively transferred to the fluid cartridge 41 above the circuit board 32, so that the sample can be subjected to the reactions such as isothermal nucleic acid amplification, polymerase Chain Reaction (PCR), and the like. After a specific reaction time (for example, 20 minutes or 30 minutes), the optical module can be used to detect the optical signal generated after the nucleic acid amplification, and the indicator 321 on the circuit board 32 can display the detection result, so that the user can directly and rapidly know that the detection has been completed through the display window 311 of the housing 31, and further automatically complete the nucleic acid detection.

In addition, in the present embodiment, the portable nucleic acid detecting apparatus 3 has only a single circuit board 32, in other words, the above-mentioned multiple necessary related chips and electronic components, for example: the heating module, the optical module, the power module, the crystal oscillator, the transmission module, the switch, the indicator lamp …, etc. can be disposed on the single circuit board 32, and can also be formed by one or more light guide plates, which is not limited thereto. Therefore, the number and arrangement positions of the circuit boards 32 in the portable nucleic acid detecting apparatus 3 can be changed according to the actual implementation, and are not limited to these embodiments.

In summary, the present utility model provides a portable nucleic acid detecting device, which is configured by tightly matching a fluid cartridge with a circuit board and a heating module thereon, so that a sample in the fluid cartridge can be subjected to isothermal nucleic acid amplification, and the nucleic acid detecting device can detect nucleic acid by an optical module correspondingly disposed on the circuit board. Therefore, the fluid cassette, the heating module, the optical module and the circuit board are arranged in a single device formed by assembling the base and the shell, so that the portable nucleic acid detection device has the advantages of compact structure, smaller volume and lighter weight, is convenient for a user to carry, can amplify and detect multiple nucleic acids anytime and anywhere, does not need to send a sample to a large laboratory for nucleic acid detection, and can greatly save detection time and cost.

Claims

1. A portable nucleic acid detecting apparatus, comprising:

a base having a receiving space;

the circuit board is arranged in the accommodating space of the base;

The heating module is provided with at least one heating component and is electrically connected with the circuit board;

An optical module having at least one light emitter and at least one light sensor and electrically connected to the circuit board;

The fluid cassette is correspondingly arranged on the circuit board and the heating module; and

The shell is correspondingly covered on the base and seals the accommodating space;

After a sample is placed in the fluid cassette, the sample is heated by the at least one heating component of the heating module, and the temperature is maintained at a set temperature, so that the sample is subjected to nucleic acid amplification, the sample is irradiated by the at least one light emitter of the optical module, the irradiated spectrum is received by the at least one light sensor to perform detection comparison, and the detection result is displayed.

2. The portable nucleic acid detecting apparatus according to claim 1, further comprising a button and a display.

3. The portable nucleic acid detecting apparatus according to claim 2, wherein the key and the display are disposed on an upper surface of the housing.

4. The portable nucleic acid testing device of claim 2, wherein the upper surface of the housing further has an opening, and the shape of the opening corresponds to a testing cartridge of the fluid cartridge.

5. The portable nucleic acid detecting apparatus according to claim 4, further comprising a cover, and the cover is sealed to the opening of the housing.

6. The portable nucleic acid detecting apparatus according to claim 2, wherein the key and the display portion are disposed on the base.

7. The portable nucleic acid detecting apparatus according to claim 1, wherein the circuit board is composed of a main circuit board and an additional circuit board, and the additional circuit board is disposed perpendicular to the main circuit board.

8. The portable nucleic acid detecting apparatus according to claim 7, wherein the additional circuit board is provided with a plurality of indicator lamps.

9. The portable nucleic acid detecting apparatus according to claim 7, further comprising a positioning seat disposed between the circuit board and the fluid cartridge, wherein a side of the positioning seat has a recess for corresponding engagement with the additional circuit board to assist positioning.

10. A portable nucleic acid detecting apparatus, comprising:

a base having a receiving space;

the circuit board is arranged in the accommodating space of the base;

The fluid cassette is correspondingly arranged on the circuit board and the heating module;

The shell is correspondingly covered on the base and is provided with an opening, and the opening corresponds to a detection box body of the fluid cassette; and

A cover cap correspondingly arranged on the opening of the shell so as to isolate the fluid cassette from the external environment;

After a sample is placed in the fluid cassette, the sample is heated by the at least one heating component of the heating module, and the temperature is maintained at a set temperature, so that the sample is subjected to nucleic acid amplification, the sample is irradiated by the at least one light emitter of the optical module, and the irradiated spectrum is received by the at least one light sensor to perform detection comparison and display a detection result.

11. The portable nucleic acid detecting apparatus according to claim 10, further comprising a button and a display portion, wherein the button and the display portion are disposed on the upper surface of the housing.