WO2023050711A1

WO2023050711A1 - Multi-channel lamp detector

Info

Publication number: WO2023050711A1
Application number: PCT/CN2022/078305
Authority: WO
Inventors: 颜菁; 俞涛
Original assignee: 江苏汇先医药技术有限公司
Priority date: 2021-09-28
Filing date: 2022-02-28
Publication date: 2023-04-06

Abstract

Disclosed is a multi-channel LAMP detector, comprising: a housing, chip ports being provided in the housing; chip bins, which are provided with a plurality of chip slots, the plurality of chip slots being arranged in parallel in the front-rear direction, and each chip slot being provided with a notch opposite to the chip ports; heating assemblies, which are used for performing constant temperature heating on microfluidic chips in the chip slots; a robotic arm mechanism, which comprises a plurality of robotic arms that can be connected to pistons of the microfluidic chips in the chip bins respectively, and the robotic arms can be movably arranged in the left-right direction and are located on the right sides of the chip bins; and an optical detection mechanism, which may be movably arranged in the housing in the left-right direction and the front-rear direction, the optical detection mechanism being provided with a plurality of detection positions, each chip slot being provided with a plurality of detection sites arranged in the left-right direction, and each detection site of each chip slot corresponds to one detection position. The present invention may process at one time samples in a plurality of microfluidic chips.

Description

A multi-channel LAMP detector

priority

This application claims the priority of the Chinese patent applications with application numbers CN2021111414091 and CN202120109588X filed on September 28, 2021.

technical field

The invention belongs to the technical field of nucleic acid detection and relates to a multi-channel LAMP detector.

Background technique

LAMP (Loop-mediated isothermal amplification, Loop-mediated isothermal amplification) technology is widely used in the field of biological diagnosis due to its mild reaction conditions (lower reaction temperature) and short reaction time, such as nucleic acid amplification detection to diagnose samples presence of pathogens. At present, LAMP technology is to provide in vitro amplification conditions for nucleic acid fragments to make them exponentially amplify in large quantities and add fluorescent dyes or fluorescent markers during the nucleic acid amplification process, and use optical devices to detect the strength of fluorescent signals. The process by which the analysis of fluorescent signals yields the results of nucleic acid amplification. During the nucleic acid amplification reaction, the reaction system needs to be heated. The current LAMP detector can integrate nucleic acid amplification detection. When the detection chip (usually a microfluidic chip) is placed in the LAMP detector, the reaction chamber (usually an amplification reaction chamber) of the detection chip can be heated, Lighting, detection, etc. When the sample volume to be detected is large, a LAMP detector capable of detecting multiple samples at one time is required.

Contents of the invention

In view of the above technical problems, an object of the present invention is to provide a multi-channel LAMP detector, which can detect samples in multiple microfluidic chips at one time, improve detection efficiency, and have a small volume.

Another object of the present invention is to provide a control method for a multi-channel LAMP detector, which automatically detects samples of multiple microfluidic chips at one time without additional device cost, thereby improving detection efficiency.

According to a first aspect of the present invention, a multi-channel LAMP detector comprises:

The shell is provided with a chip port for inserting the microfluidic chip;

A chip chamber, which is arranged in the housing, and the chip chamber has a plurality of chip slots for accommodating microfluidic chips, and the plurality of chip slots are arranged side by side along the front and rear directions, and each of the chip slots Respectively have notches opposite to the chip ports;

a heating component, which is used for constant temperature heating of the microfluidic chip in the chip slot;

A mechanical arm mechanism, which includes a plurality of mechanical arms capable of engaging with the pistons of the microfluidic chips in the chip chamber, each of the chip slots corresponds to at least one of the mechanical arms, and each of the mechanical arms can move along the left and right is arranged in the casing to move in direction and is located on the right side of the chip compartment; and

An optical detection mechanism, which can be moved in the left-right direction and the front-rear direction, is arranged in the housing, and the optical detection mechanism is located below the chip compartment;

Wherein, the optical detection mechanism has a plurality of detection positions, each of the chip slots has a plurality of detection positions arranged along the left and right directions, and each of the detection positions of each of the chip slots corresponds to one The detection position.

According to a preferred embodiment, the multi-channel LAMP detector also includes:

a door, which is used to close the chip opening, and the door is movably arranged on the inner wall of the housing;

The first driving mechanism is used to drive the door to move.

According to a preferred embodiment, the inner wall of the housing is provided with a first guide rail, the door is movably arranged on the first guide rail, the first drive mechanism includes a first motor and is driven by the first guide rail. A motor drives a rotating first threaded rod, and the first threaded rod is connected with the door.

According to a preferred embodiment, the multi-channel LAMP detector further includes a chip cover, and the chip cover is arranged between the inner wall of the housing surrounding the chip opening and the chip chamber and between the chip slots, The gap between the chip chamber and the inner wall of the casing and the gap between the chip slots are closed.

More preferably, the multi-channel LAMP detector also includes a code scanner for reading the barcode on the microfluidic chip, the code scanner is arranged in the housing, and the housing is provided with a scanning window , the scanning part of the code scanner is located in the scanning window or facing the scanning window.

Further, the multi-channel LAMP detector also includes a controller and a touch screen, the controller is electrically connected to the scanner and the touch screen respectively, and the touch screen has a first A display state, in the first display state, the touch screen has a code scanning input button.

According to a preferred embodiment, each of the chip sockets corresponds to at least one of the heating components, and the heating components are arranged on the side walls of the chip sockets.

More preferably, the mechanical arm mechanism includes a bracket disposed on the inner wall of the housing, a lower mounting plate capable of being disposed on the bracket along the left-right direction, and an upper mounting plate capable of being moved along the left-right direction on the bracket. plate, the upper mounting plate is located above the lower mounting plate, the upper mounting plate is provided with a plurality of first mechanical arms, and the lower mounting plate is provided with a plurality of second mechanical arms, each of the The chip sockets correspond to at least one first robot arm and at least one second robot arm respectively, and at an initial position, the second robot arm is located at the lower left side of the first robot arm.

According to a preferred embodiment, the first mechanical arm and/or the second mechanical arm respectively have engagement grooves for the piston of the microfluidic chip to snap into, and the engagement grooves have notches facing upward.

According to a preferred embodiment, the mechanical arm mechanism further includes a second drive mechanism for driving the upper mounting plate to move left and right, and a third drive mechanism for driving the lower mounting plate to move left and right, and the bracket A second guide rail and a third guide rail extending in the left-right direction are arranged on the top, the upper mounting plate is arranged on the second guide rail so as to be movable in the left-right direction, and the lower mounting plate is arranged on the second guide rail so as to be movable in the left-right direction. On the third guide rail, the second driving mechanism includes a second motor and a second screw driven by the second motor, the second screw is connected to the upper mounting plate, and the third The driving mechanism includes a third motor and a third lead screw driven and rotated by the third motor, and the third lead screw is connected with the lower mounting plate.

According to a preferred embodiment, the mechanical arm mechanism further includes a first photoelectric switch for detecting whether the first mechanical arm has reached its initial position, and a photoelectric switch for detecting whether the second mechanical arm has reached its initial position. The second photoelectric switch.

According to a preferred embodiment, the bracket has a blocking portion for preventing the upper mounting plate and the lower mounting plate from moving rightward beyond their set strokes.

According to a preferred embodiment, the multi-channel LAMP detector includes a base provided on the inner wall of the housing, and the base has an X guide rail extending along the left and right directions; the multi-channel LAMP detector also includes a An installation block arranged on the X-guiding rail to move left and right, the installation block has a Y-guiding rail extending along the front-rear direction; the optical detection mechanism is arranged on the Y-guiding rail so as to be able to move along the front-rear direction.

According to a preferred embodiment, the multi-channel LAMP detector further includes a fourth driving mechanism for driving the mounting block to move along the X-guiding rail, and a fourth driving mechanism for driving the optical detection mechanism to move along the Y-guiding rail. The fifth driving mechanism for moving, the X-direction start detection switch for detecting whether the installation block reaches its starting point, the X-direction end detection switch for detecting whether the installation block reaches its end point, and the X-direction end detection switch for detecting whether the optical Whether the detection mechanism reaches the Y-direction starting point detection switch and the Y-direction end-point detection switch for detecting whether the optical detection mechanism reaches its end point, the X-direction start-point detection switch and the X-direction end-point detection switch are respectively The Y-direction start detection switch and the Y-direction end detection switch are respectively close to the two ends of the Y-direction guide rail.

According to a preferred embodiment, the multi-channel LAMP detector also includes a PCB board, a mounting frame is provided in the housing, a guide chute is provided on the mounting frame, and the edge of the PCB board is inserted into the in the guide chute.

The utility model adopts the above scheme, and has the following advantages compared with the prior art:

A multi-channel LAMP detector of the utility model is provided with multiple chip slots in the chip chamber, which can process multiple microfluidic chips at one time, and the number of samples that can be processed once the detection program is run is greatly increased, and has High detection efficiency; moreover, multiple detection channels of the LAMP detector can share some components, such as mechanical arm mechanism, optical detection mechanism, etc. Compared with the single-channel LAMP detector, the space is effectively used, and the volume of the whole machine is smaller. It will not increase significantly, and will not take up too much space.

Description of drawings

In order to illustrate the technical solution of the present invention more clearly, the accompanying drawings that need to be used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings in the following description are only some embodiments of the present invention. Ordinary technicians can also obtain other drawings based on these drawings on the premise of not paying creative work.

Fig. 1 is the overall structure schematic diagram of a kind of multi-channel LAMP detector of the present embodiment;

Fig. 2 is a schematic diagram of the internal structure of a multi-channel LAMP detector of the present embodiment;

FIG. 3 is a schematic diagram of the internal structure of another viewing angle of a multi-channel LAMP detector of the present embodiment;

FIG. 4 is an internal schematic diagram of a housing of a multi-channel LAMP detector of the present embodiment;

FIG. 5 is a schematic structural diagram of the scanner bracket of this embodiment;

FIG. 6 is a structural schematic diagram of another viewing angle of the scanner bracket of this embodiment;

FIG. 7 is a schematic structural view of the first heating module and the second heating module of this embodiment;

Fig. 8 is a structural schematic view of another viewing angle of the first heating module and the second heating module of this embodiment;

FIG. 9 is a schematic structural view of the mechanical arm mechanism of this embodiment;

Fig. 10 is another structural schematic diagram of the mechanical arm mechanism of this embodiment;

Fig. 11a is an axonometric view of the optical detection mechanism of this embodiment;

Fig. 11b is a top view of the optical detection mechanism of this embodiment;

FIG. 12 is a schematic structural view of the mounting frame of the present embodiment;

FIG. 13 is a schematic structural view of the microfluidic chip provided by the LAMP detector of this embodiment;

FIG. 14 is a schematic structural diagram of the chip cap of this embodiment;

FIG. 15a and FIG. 15b are schematic structural views of a chip socket in this embodiment;

Fig. 16 is a schematic diagram of detection sites in this embodiment.

in,

1-shell; 2-chip warehouse; 3-door; 4-chip cover; 6-heating component; 7-mechanical arm mechanism; 8-optical detection mechanism; 10-chip slot; 11-base; 12-foot ; 13-connection hole;

100-microfluidic chip; 100a-piston; 100a1-neck;

101-chip port; 102-notch;

301-the first guide rail; 302-the first motor; 303-the first screw rod;

501-code scanner; 502-code scanner bracket; 5021-base; 5022-support table; 503-scanning window; 504-display screen;

601-the first heating module; 6011-the first insulation cotton compact; 6012-the first heating block; 6013-the first heating film; 6014-the first heat insulation sheet; 6015-the first insulation cotton; 602-the second heating Module; 6021-the second insulation cotton compact; 6022-the second heating block; 6023-the second heating film; 6024-the second heat insulation sheet; 6025-the second insulation cotton; 603-the first temperature sensor; 604-the first Two temperature sensors;

701-bracket; 7011-blocking part; 702-upper mounting plate; 703-lower mounting plate; 704-first mechanical arm; 7041-first photoelectric switch induction bracket; 705-second mechanical arm; 7051-second photoelectric switch Induction bracket; 706-joint groove; 7071-second motor; 7072-second screw; 7081-third motor; 709-second guide rail; 711-first photoelectric switch; 712-second photoelectric switch;

801-X direction start detection switch; 802-X direction end detection switch; 803-Y direction start detection switch; 804-Y direction end detection switch; 805-X direction motor; 806-Y direction motor; 809-X direction rail; 810-Y guide rail;

91-main PCB board; 92-manipulator control PCB board; 93-door control PCB board;

101-mounting frame; 101a-guiding chute.

Detailed ways

The preferred embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings, so that the advantages and features of the present invention can be more easily understood by those skilled in the art. It should be noted here that the descriptions of these embodiments are used to help understand the present invention, but are not intended to limit the present invention.

In the description of the present application, it should be understood that the terms "upper", "lower", "left", "right", "front", "rear", "vertical", "horizontal", "inner" and " The orientation or positional relationship indicated by "outside" and so on is based on the orientation or positional relationship shown in Figures 1 and 2, which is only for the convenience of describing the application and simplifying the description, rather than indicating or implying that the referred device or element must have a specific Orientation, construction and operation in a particular orientation and therefore should not be construed as limiting the application.

As shown in this specification and claims, the terms "comprising" and "comprising" only suggest the inclusion of explicitly identified steps and elements, and these steps and elements do not constitute an exclusive list, and the method or device may also include other steps or elements. As used herein, the term "and/or" includes any combination of one or more of the associated listed items.

It should be noted that, unless otherwise specified, when a feature is called "fixed" or "connected" to another feature, it can be directly fixed and connected to another feature, or indirectly fixed and connected to another feature. on a feature.

Unless the context clearly dictates otherwise, the indefinite articles "a", "an" and "an" preceding an element or component herein are to be read as including one or at least one, and singular forms of said element or component also include plural form.

It can be further understood that the terms "first", "second", etc. are used to describe various information, but the information should not be limited to these terms. These terms are only used to distinguish information of the same type from one another, and do not imply a specific order or degree of importance. In fact, expressions such as "first" and "second" can be used interchangeably. For example, without departing from the scope of the present disclosure, first information may also be called second information, and similarly, second information may also be called first information.

FIG. 1 to FIG. 16 show a multi-channel LAMP detector according to this embodiment, which can automatically perform nucleic acid amplification detection based on the LAMP reaction. As shown in FIG. 1 , the LAMP detector includes a housing 1 , an inner cavity for installing internal components is formed in the housing 1 , and a chip port 101 for inserting a microfluidic chip 100 is provided on the housing 1 . The housing 1 is provided with a chip chamber 2 , and the chip chamber 2 has a plurality of chip slots 10 for accommodating microfluidic chips 100 . Specifically in this embodiment, there are three chip slots 10 inside the chip chamber 2, and each chip slot 10 corresponds to a detection channel. In the chip warehouse 2, a plurality of chip slots 10 are arranged side by side along the front and rear direction, and each chip slot 10 has a notch 102 opposite to the chip opening 101, specifically, the chip slot 10 is located directly below the chip opening 101 , the notch of the chip socket 10 faces upward, and the microfluidic chip 100 is inserted into the chip socket 10 from top to bottom.

As shown in FIG. 1 and FIG. 4 , the multi-channel LAMP detector further includes a door 3 movably arranged on the inner wall of the housing 1 , and the door 3 is used to close the chip opening 101 . Specifically, the door 3 is movably disposed on the inner wall of the upper side of the casing 1 . Further, a first guide rail 301 extending in the left-right direction is provided on the upper inner wall of the casing 1 , and the door 3 is movably arranged on the first guide rail 301 through a slider. The door 3 is driven to open and close by the first driving mechanism. Specifically, the first driving mechanism includes a first motor 302 and a first screw 303 driven to rotate by the first motor 302 , and the first screw 303 is connected to the door 3 through threads (for example, through a screw nut). The first screw 303 can be driven by the first motor 302 to rotate, and through the cooperation of the screw and the screw nut, the rotational motion of the first motor 302 is converted into linear motion, so that the door 3 can move left and right. There are two first guide rails 301 parallel to each other, and they are respectively arranged on the front and rear sides of the door 3 . The opening and closing of the door 3 is detected by the door sensor, the door sensor is electrically connected to a door controller, and the door controller is also electrically connected to the first motor 302; when the door sensor is triggered, a detection indicating that the door 3 is opened or closed is sent out After receiving the detection signal, the door controller sends a control signal to the first motor 302 to control its forward rotation or reverse rotation, thereby driving the door 3 to close or open. The door controller is arranged on a door control PCB 93 , and the PCB 93 is arranged on the inner wall of the casing 1 . After the microfluidic chip is loaded, the door 3 is automatically closed to seal the chip port 101 to protect the internal components, which is more beautiful.

Referring to FIG. 1 , FIG. 2 , FIG. 5 and FIG. 6 , the multi-channel LAMP detector further includes a code scanner 501 for reading the barcode on the microfluidic chip 100 . The code scanner 501 is installed in the housing 1 through the code scanner bracket 502 . The housing 1 is provided with a scanning window 503 . The scanning part of the code scanner 501 is located in the scanning window 503 or facing the scanning window 503 . The scanner bracket 502 includes a base 5021 and a supporting platform 5022 arranged in parallel on the base 5021 . The supporting platform 5022 is wedge-shaped. A connecting hole 13 is also provided on the base 5021 , and the scanner bracket 502 is fixedly arranged on the inner wall of the housing 1 through a connecting piece passing through the connecting hole 13 . Both the chip port 101 and the code scanner 501 are located on the upper side of the housing 1 and both face upward.

The multi-channel LAMP detector also includes a main controller and a display screen 504 (specifically, a touch screen), and the main controller is electrically connected to the code scanner 501 and the display screen 504 respectively. The main controller is arranged on the main PCB board 91 . The display screen 504 has a first display state, and in the first display state, the display screen 504 has a code scanning input button. After pressing the code scanning input button for the first time, the information of the microfluidic chip 100 read by the code scanner 501 is associated with the first detection channel (for example, the chip slot 10 on the front side); After scanning the code input button, the information of the microfluidic chip 100 read by the code scanner 501 is associated with the second detection channel (for example, the chip slot 10 in the middle); after pressing the code scanning input button for the third time, The information of the microfluidic chip 100 read by the scanner 501 is associated with the third detection channel (eg, the last chip slot 10 ). The display screen 504 is located on the left side of the housing 1 .

Referring to FIG. 7 to FIG. 8 , the multi-channel LAMP detector further includes a heating assembly for heating the microfluidic chip 100 in the chip slot 10 at a constant temperature. Specifically, the heating assembly is used to heat the reaction chamber of the microfluidic chip 100 . Each chip socket 10 corresponds to at least one heating component, and the heating component is attached to the side wall of the chip socket 10 . Each heating assembly includes a first heating module 601 and a second heating module 602, and the first heating module 601 includes a first thermal insulation cotton pressing block 6011, a first heating block 6012, a first heating film 6013, a first thermal insulation cotton 6015 and a first thermal insulation cotton 6015. A thermal insulation sheet 6014 , the second heating module 602 includes a second thermal insulation cotton pressing block 6021 , a second heating block 6022 , a second heating film 6023 , a second thermal insulation cotton 6025 and a second thermal insulation sheet 6024 . The structures of the first heating module 601 and the second heating module 602 are similar, and the difference is that the shapes of the second thermal insulation cotton pressing block 6021 and the second heating block 6022 of the second heating module 602 are similar to those of the first heating block 602 of the first heating module 601. The shape of the thermal insulation cotton pressing block 6011 and the first heating block 6012 is different. Taking the first heating module 601 as an example here, the first heating block 6012 and the first heat insulation sheet 6014 are covered and arranged on the first heating film 6013. The surface of the first heating film 6013 has an adhesive layer, and the first heating film 6013 is bonded Covering and setting on the front of the first thermal insulation cotton 6015, the back of the first thermal insulation cotton 6015 is provided with a first thermal insulation cotton compact 6011, the first thermal insulation cotton compact 6011 has a connection hole 13, through the connection hole 13 The connecting member disposes the first heating module 601 on the sidewall of the chip socket 10 . The first temperature sensor 603 is fixedly arranged on the first heating block 6012, and the second temperature sensor 604 is fixedly arranged on the second heating block 6022. The first temperature sensor 603 and the second temperature sensor 604 are connected with a controller (which can be the main controller) electrical connection. The controller is used to correspondingly control the magnitude of the voltage supplied to the first heating film 6013 according to the temperature information detected by the first temperature sensor 603, thereby maintaining the heating temperature of the first heating block 6012 constant; The temperature information detected by the second temperature sensor 604 controls the magnitude of the voltage supplied to the second heating film 6023 accordingly, so as to keep the heating temperature of the second heating block 6022 constant.

Fig. 15a and Fig. 15b show the partial structure of a single chip socket 10 in the chip chamber, in which the microfluidic chip 100 can be inserted. As shown in Figure 15a, a hollow area 10a is provided on the side wall of the chip socket 10, so as to cooperate with the heating assembly; Cooperate. The first heating block 6012 matches the shape of a hollowed out area 10a on the upper part of the side wall of the chip socket 10, and is embedded in the hollowed out area 10a, so that it can be attached to the microfluidic chip in the chip socket 10; similarly The second heating block 6022 matches the shape of a hollowed-out area 10a at the lower part of the side wall of the chip socket 10, and is embedded in the hollowed-out area 10a, so that it can be attached to the microfluidic chip in the chip socket 10. In addition, the part that is not attached to the microfluidic chip is provided with a thermal insulation sheet, that is, the high-temperature component of the first heating module 601 is separated from the side wall of the chip socket 10 by the first thermal insulation sheet 6014, and the second insulation sheet 6014 The thermal sheet 6024 separates the high-temperature components of the second heating module 602 from the sidewall of the chip socket 10 to avoid the problem of premature aging caused by the chip socket 10 being exposed to a high-heat environment for a long time.

9 and shown in FIG. 10, the multi-channel LAMP detector also includes a mechanical arm mechanism 7, the mechanical arm mechanism 7 includes a plurality of mechanical arms that can engage with the piston 100a of the microfluidic chip 100 in the chip warehouse 2, and the mechanical arm Used to drive the piston 100a to move. Each chip slot 10 corresponds to at least one mechanical arm, and each mechanical arm is arranged in the casing 1 and is located on the right side of the chip magazine 2 so as to be movable in the left and right directions. The mechanical arm mechanism 7 includes a bracket 701 arranged on the inner wall of the housing 1, an upper mounting plate 702 that can be moved along the front and rear directions on the bracket 701, and a lower mounting plate 703 that can be arranged on the bracket 701 along the left and right directions. 702 is located above the lower mounting plate 703. The upper mounting plate 702 is provided with a plurality of first mechanical arms 704, and the lower mounting plate 703 is provided with a plurality of second mechanical arms 705. Each chip slot 10 is respectively connected to at least one of the first mechanical arms. A robot arm 704 and at least one second robot arm 705 correspond to each other. In the initial position, the second robot arm 705 is located at the lower left of the first robot arm 704 .

Referring to Fig. 9, Fig. 10 and Fig. 13, the first mechanical arm 704 and the second mechanical arm 705 respectively have holes for the end of the piston 100a of the microfluidic chip 100 (specifically, the neck 100a1 of the piston 100a) to snap into. Engagement slot 706 having notch 102 facing upwards. When viewed from above, it can be seen that the first mechanical arm 704 and the second mechanical arm 705 are spaced apart from each other in front, rear, left and right, and can be combined with two pistons 100a at the same time. Specifically, the second mechanical arm 705 is located at the lower rear of the first mechanical arm 704 , that is, the second mechanical arm 705 is located at a distance from the rear side of the first mechanical arm 704 to match the positions of the two pistons 100 a.

9 and 10, the mechanical arm mechanism 7 also includes a second drive mechanism for driving the upper mounting plate 702 to move left and right, and a third drive mechanism for driving the lower mounting plate 703 to move left and right. There are second guide rails 709 and third guide rails (not shown) extending in the left-right direction. The upper mounting plate 702 is arranged on the second guide rail 709 to be movable in the left-right direction, and the lower mounting plate 703 is movable in the left-right direction. Set on the third guide rail, the second driving mechanism includes a second motor 7071 and a second screw 7072 driven by the second motor 7071, the second screw 7072 is connected to the upper mounting plate 702, and the third driving mechanism includes the second Three motors 7081 and a third lead screw (not shown) driven by the third motor 7081 to rotate, the third lead screw is connected with the lower mounting plate 703 . The mechanical arm mechanism 7 also includes a first photoelectric switch 711 for detecting whether the first mechanical arm 704 has reached its initial position, and a second photoelectric switch 712 for detecting whether the second mechanical arm 705 has reached its initial position. The bracket 701 has a blocking portion 7011 for preventing the upper mounting plate 702 and the lower mounting plate 703 from moving rightward beyond their set stroke. The second motor 7071 and the third motor 7081 are also respectively electrically connected to the controller of the robotic arm. The controller of the robotic arm is used to send control signals to control the operation of the second motor 7071 and the third motor 7081 respectively. A photoelectric switch 711 is electrically connected to the second photoelectric switch 712 to receive signals from the first photoelectric switch 711 and the second photoelectric switch 712 . The robotic arm controller is located on the robotic arm control PCB 92 . This embodiment can precisely control the first mechanical arm 704 and the second mechanical arm 705, the three first mechanical arms 704 are controlled by the same drive system, and the three second mechanical arms 705 are controlled by the same drive system, which can synchronously control the three micro The movement of the piston of the fluidic chip 100 has high control precision and avoids excessive cost increase.

The controller of the mechanical arm will send a control signal to the second motor 7071 to make the second motor 7071 start to run. The second motor 7071 drives the second screw rod 7072 to rotate and drives the upper mounting plate 702 to move linearly. The upper mounting plate 702 is provided with a first mechanical arm 704 , and the first mechanical arm 704 will move linearly along with the upper mounting plate 702 . The first mechanical arm 704 is provided with a first photoelectric switch sensing bracket 7041, and the first photoelectric switch sensing bracket 7041 will move linearly along the second guide rail 709 with the first mechanical arm 704 until the first photoelectric switch sensing bracket 7041 moves to At the first photoelectric switch 711, the first photoelectric switch 711 is triggered to send a detection signal, which is transmitted to the controller of the robotic arm. After receiving the detection signal, the controller of the robotic arm controls the second motor 7071 to run in reverse or stop. When the second motor 7071 is running, the second screw 7072 moves forward or backward along the front-back direction, and the first mechanical arm 704 moves forward or backward along the second guide rail 709, thereby driving the upper part of the microfluidic chip 100 The piston 100a moves left and right, and then provides positive or negative pressure to the liquid in the microfluidic chip 100, thereby providing power for liquid circulation.

Similarly, the controller of the robotic arm sends a control signal to the third motor 7081 to make the third motor 7081 start to run. The third motor 7081 drives the third screw to rotate and drives the lower mounting plate 703 to move linearly. A second mechanical arm 705 is disposed on the lower mounting plate 703 , and the second mechanical arm 705 will move linearly along with the lower mounting plate 703 . The second mechanical arm 705 is provided with a second photoelectric switch induction bracket 7051 . The second photoelectric switch sensing bracket 7051 will move linearly along the third guide rail with the second mechanical arm 705 until the second photoelectric switch sensing bracket 7051 moves to the second photoelectric switch 712, and the second photoelectric switch 712 is triggered to send a detection The signal is transmitted to the controller of the robotic arm. After receiving the detection signal, the controller of the robotic arm controls the third motor 7081 to run in reverse or to stop running. When the third motor 7081 is running, the third screw rod moves left or right along the left and right directions, and the second mechanical arm 705 moves left or right along the third guide rail, thereby driving the lower piston of the microfluidic chip 100 100a moves left and right, and then provides positive pressure or negative pressure to the liquid in the microfluidic chip 100 to provide power for liquid circulation, or switch the connection between chambers in the microfluidic chip 100 .

11a and 11b, the multi-channel LAMP detector includes an optical detection mechanism 8, the optical detection mechanism 8 can be moved along the left and right direction and the front and rear directions in the housing 1, the optical detection mechanism 8 is located in the chip chamber 2 Below, each amplification detection chamber of the microfluidic chip 100 in each chip slot 10 is detected one by one in order, wherein a plurality of microfluidic chips 100 are arranged along the front and rear directions, and each microfluidic chip 100 has a A plurality of amplification detection chambers arranged at intervals in the direction, each amplification detection chamber is a detection site, as shown in FIG. 16 . For example, the optical detection mechanism 8 moves along the left and right direction, and can scan multiple detection sites of the microfluidic chip 100 in a certain chip slot 10 one by one; It is aligned with another microfluidic chip 100 , and then moves in the left and right directions to scan the multiple detection sites of the microfluidic chip 100 one by one.

Further, the multi-channel LAMP detector also includes a base 11 arranged on the inner wall of the housing 1, the base 11 has an X guide rail 809 extending in the left and right directions; the multi-channel LAMP detector also includes a device capable of moving in the left and right directions. For the installation block on the X guide rail 809, the installation block has a Y guide rail 810 extending along the front-rear direction; The multi-channel LAMP detector also includes an X-direction driving mechanism for driving the mounting block to move along the X-guiding rail 809, a Y-direction driving mechanism for driving the optical detection mechanism 8 to move along the Y-guiding rail 810, and a Y-direction driving mechanism for detecting the optical detection mechanism 8 to move along the Y-guiding rail 810. Whether the detection mechanism 8 arrives at its starting point on the left-right direction to the X-direction detection switch 801, whether it is used to detect whether the optical detection mechanism 8 reaches its end point on the left-right direction to the X-direction detection switch 802, for detecting the optical detection Whether mechanism 8 arrives at its starting point on the front-back direction to the Y direction detection switch 803, and is used to detect whether the optical detection mechanism 8 arrives at its Y direction end point detection switch 804 on the front-back direction, X to the starting point detection switch 801 and X-direction end detection switch 802 are respectively close to both ends of X-direction guide rail 809 , and Y-direction start detection switch 803 and Y-direction end detection switch 804 are respectively close to two ends of Y-direction guide rail 810 . Specifically in this embodiment, the X-direction drive mechanism includes an X-direction motor 805, and the X-direction motor 805 is arranged on the base 11 and drives the Y guide rail 810 to move left and right through a screw and a nut; the Y-direction drive mechanism includes a Y-direction motor 806 , the Y-direction motor 806 is arranged on the mounting block and drives the optical detection mechanism 8 to move forward and backward along the Y-direction rail through a screw rod and a nut. The X-direction start detection switch 801 , the X-direction end detection switch 802 , the Y-direction start detection switch 803 , and the Y-direction end detection switch 804 are photoelectric detection switches, respectively. Referring to Fig. 16, the X-direction start detection switch 801 sends out a first detection signal when it detects that the optical detection mechanism 8 reaches its starting point in the left-right direction, and the X-direction end detection switch 802 is used to detect that the optical detection mechanism 8 arrives. It sends the second detection signal when the end point on the left-right direction, Y to the starting point detection switch 803 for sending the third detection signal when detecting that the optical detection mechanism 8 arrives at its starting point on the front-back direction, Y to the end point detection switch 804 It is used for sending out a fourth detection signal when it is detected that the optical detection mechanism 8 reaches its end point in the front-rear direction.

The optical detection mechanism 8 has a plurality of detection positions, and each chip socket 10 has a plurality of detection positions arranged along the left and right directions, and each detection position of each chip socket 10 corresponds to one detection position. Specifically in this embodiment, as shown in Figure 16, the microfluidic chip in each chip slot 10 corresponds to one detection channel, a total of three detection channels C1, C2 and C3, and each chip slot 10 has four detection sites, there are three chip slots 10, so there are twelve detection sites; specifically, detection channel C1 has four detection sites P11, P12, P13 and P14, and detection channel C2 has four detection sites Sites P21, P22, P23 and P24; detection channel C3 has four detection sites P31, P32, P33 and P34. Taking the chip slot 10 at the front as an example, the optical detection mechanism 8 starts to run from the origin, and moves according to the coordinates formed by the detection points. After the optical detection mechanism 8 passes through four detection points, the optical detection mechanism 8 Move to the next chip socket 10 along the Y direction, detect the second chip socket 10, and so on.

The main controller is electrically connected to the X-direction starting point detection switch 801, the X-direction end point detection switch 802, the Y-direction starting point detection switch 803, and the Y-direction end point detection switch 804, and controls the X-direction driving mechanism to move forward after receiving the first detection signal. To run, and after receiving the second detection signal, control the X-direction drive mechanism to run in reverse, and after receiving the third detection signal, control the Y-direction drive mechanism to run forward, and after receiving the fourth detection signal The Y-direction driving mechanism is controlled to run in reverse.

Referring to FIG. 3 and FIG. 12 , the housing 1 is provided with a mounting frame 101 , and the mounting frame 101 is located between the display screen 504 and the chip compartment 2 . The installation frame 101 is provided with a guide chute 101a, and the edge of the main PCB board 91 is inserted into the guide chute 101a. The PCB board can be installed in a simple structure and low-cost manner, the volume is small, and the installation is convenient, especially in the case of a relatively narrow installation space, the assembly and maintenance are relatively convenient.

As shown in Figure 14, the multi-channel LAMP detector also includes a chip cover 4, and the chip cover 4 is arranged between the inner wall of the housing 1 around the chip port 101 and the chip chamber 2 and between the chip slots 10, and its hollowed out part and The slots of each chip socket 10 correspond to allow the microfluidic chip 100 to be inserted into the chip socket 10 . The chip cover 4 is used to seal the gap between the chip chamber 2 and the inner wall of the housing 1 and the gap between the chip slots 10 to prevent foreign matter from falling in, prevent dust, and protect internal components. The chip cover 4 can also play a role of shielding the parts inside the tester, which is more beautiful. The chip cap 4 adopts 3D printing technology, which has a simple structure and can solve the problem of high manufacturing cost.

The working process of the multi-channel LAMP detector of the present embodiment is as follows:

After aligning the microfluidic chip 100 with the code scanner 501 and scanning the code, insert it into the chip slot 10, the door 3 is automatically closed, and the first mechanical arm 704 and the second mechanical arm 705 of the mechanical arm mechanism 7 follow the set sequence. Move, drive the piston 100a to move, communicate with the cavity, and provide positive or negative pressure for fluid flow, so that the sample and reagent of the microfluidic chip 100 are mixed or the reagent is circulated into the target chamber until it enters the reaction chamber for further processing. Amplification reaction; power on the heating component to heat the corresponding chamber of the microfluidic chip 100; after the reaction, the optical detection mechanism 8 is powered on, and moves to each detection position in sequence according to the set time sequence, for each An amplification detection chamber is used for optical detection.

The present embodiment also provides a control method of a multi-channel LAMP detector, which includes the following process:

A. Scan the code of the microfluidic chip 100 to enter the sample information and associate it with the corresponding detection channel C1, C2 or C3, that is, install the microfluidic chip 100;

B. Heating the microfluidic chip 100 at a constant temperature:

C. Control the movement of the first mechanical arm 704 and the second mechanical arm 705, so that the reaction systems such as samples and primers of the microfluidic chip 100 are mixed and distributed to each amplification chamber;

D. Control the movement of the optical detection mechanism 8 to detect the detection points one by one.

Referring to Figure 16, process D specifically includes the following steps:

S101, the optical detection mechanism 8 is at the origin position or moved to the origin position (that is, the detection point P11), the X-direction starting point detection switch 801 and the Y-direction starting point detection switch 803 are both triggered to send out the first detection signal and the third detection signal, after receiving the first detection signal and the third detection signal, the main controller judges that the optical detection mechanism 8 is at the origin, and detects the detection point P11 of the first microfluidic chip 100 . The main controller establishes a corresponding relationship between the detection information sent by the optical detection mechanism 8 at this time and the detection point P11 of the first detection channel C1.

S102, the main controller controls the X-direction motor 805 to run forward, so that the optical detection mechanism 8 moves to the right for a set distance d1 and then stops to the detection point P12 for detection; specifically, by setting the distance d1 (that is, two adjacent distance between detection points) to convert the rotation angle of the X-direction motor 805, and stop when the X-direction motor 805 turns over the set angle. The main controller establishes a corresponding relationship between the detection information sent by the optical detection mechanism 8 at this time and the detection point P12 of the first detection channel C1.

By analogy, control the optical detection mechanism 8 to sequentially move to the detection positions P13 and P14 for detection, and the main controller sequentially establishes the detection information sent by the optical detection mechanism 8 with the detection positions P13 and P14 of the first detection channel C1 Correspondence.

When detecting the detection point P14 of the first microfluidic chip 100 , the X-direction endpoint detection switch 802 is triggered to send a second detection signal.

S103. After the main controller receives the second detection signal and waits for the set time, it controls the X-direction motor 805 to run in reverse, so that the optical detection mechanism 8 moves leftward to return to the origin, and at this time the X-direction starting point detection switch 801 is triggered The first detection signal is issued; the set time is at least longer than the time required by the optical detection mechanism 8 to complete the optical detection of one detection point.

S104, after the optical detection mechanism 8 returns to the origin, the main controller receives the first detection signal and the third detection signal, controls the Y-direction motor 806 to move backward by a set distance d2, and then stops the detection of the second microfluidic chip 100 The main controller establishes a corresponding relationship between the detection information sent by the optical detection mechanism 8 at this time and the detection point P21 of the second detection channel C2. Specifically, the rotation angle of the Y-direction motor 806 is converted by setting the distance d2 (that is, the distance between two adjacent microfluidic chips 100), and the Y-direction motor 806 stops when it rotates through the set angle.

S105, the main controller controls the X-direction motor 805 to run forward, so that the optical detection mechanism 8 moves to the right for a set distance d1 and then stops to the detection position P22 for detection; The information establishes a corresponding relationship with the detection point P22 of the second detection channel C2.

By analogy, the optical detection mechanism 8 is controlled to move to the detection positions P23 and P24 in turn for detection, and the main controller sequentially establishes the detection information sent by the optical detection mechanism 8 with the detection positions P23 and P24 of the second detection channel C2. Correspondence.

When detecting the detection point P24 of the second microfluidic chip 100 , the X-direction endpoint detection switch 802 is triggered to send a second detection signal.

S106. After receiving the second detection signal and waiting for the set time, the main controller controls the X-direction motor 805 to run in reverse, so that the optical detection mechanism 8 moves to the left until the X-direction starting point detection switch 801 is triggered to send out the first detection signal. A heartbeat.

S107, after the optical detection mechanism 8 returns to the origin, the main controller receives the first detection signal, and after the main controller receives the first detection signal, controls the Y-direction motor 806 to move backward d2 to the detection of the last microfluidic chip 100 The main controller establishes a corresponding relationship between the detection information sent by the optical detection mechanism 8 at this time and the detection point P31 of the third detection channel C3.

S108, the main controller controls the X-direction motor 805 to run forward, so that the optical detection mechanism 8 moves to the right for a set distance d1 and then stops to the detection position P32 for detection; The information establishes a corresponding relationship with the detection point P32 of the third detection channel C3.

By analogy, the optical detection mechanism 8 is controlled to move to the detection positions P33 and P34 in sequence for detection, and the main controller sequentially establishes the detection information sent by the optical detection mechanism 8 with the detection positions P33 and P34 of the third detection channel C3. corresponding relationship.

When detecting the detection point P44 of the third microfluidic chip 100 , the X-direction endpoint detection switch 802 is triggered to send a second detection signal.

S109. After receiving the second detection signal and waiting for the set time, the main controller controls the X-direction motor 805 to run in reverse, so that the optical detection mechanism 8 moves to the left until the X-direction starting point detection switch 801 is triggered to send out The first detection signal; meanwhile, in the process of detecting the third detection channel, the Y-to-end detection switch 804 is triggered to send the fourth detection signal;

S110. After the main controller receives the first detection signal and the fourth detection signal, it controls the Y direction motor 806 to run in reverse, so that the optical detection mechanism 8 moves forward until the X direction starting point detection switch 801 and the Y direction starting point detection switch 803 are both Triggered to send out the first detection signal and the third detection signal, the Y-direction motor 806 stops running, so that the optical detection mechanism 8 returns to the origin and waits for the next detection.

Process A specifically includes:

S201. Switch the display screen 504 to the first display state, receive the user's first scan code input instruction through the display screen 504, and read the first bar code information of the first microfluidic chip 100 through the code scanner 501, and the main The controller associates the first barcode information with the first detection channel C1 after receiving the first code scanning input instruction and the first barcode information;

S202. Receive the user's second code scanning input instruction through the display screen 504, and read the second barcode information of the second microfluidic chip 100 through the code scanner 501. After receiving the second scanning code, the main controller After the code input instruction and the second barcode information, associate the second barcode information with the second detection channel C2;

S203. Receive the user's third code scanning input instruction through the display screen 504, and read the third barcode information of the third microfluidic chip 100 through the code scanner 501. After receiving the third scanning code, the main controller After the code input instruction and the second barcode information, associate the third barcode information with the third detection channel C3.

The above-mentioned barcode information includes the information of the sample to be tested, such as serial number, corresponding subject's name, source and so on.

After the detection is completed, the display screen 504 is switched to the second display state, and the detection results (yin/yang) of each detection site, the associated number, the name of the subject, etc. are displayed.

In the multi-channel LAMP detector provided in this embodiment, the chip chamber 2 is provided with multiple chip slots 10, which can detect multiple chips at the same time, and can process multiple microfluidic chips 100 at one time. The detection procedure The number of samples that can be processed in one operation is greatly improved, and has a high detection efficiency; the door 3 and the chip cover 4 can prevent dust and foreign matter from entering the gap between the chip chamber 2 and the inner wall, and the chip cover 4 can also It can shield the components inside the detector and make the appearance more beautiful; multiple detection channels of the LAMP detector can share some components, such as the mechanical arm mechanism, optical detection mechanism 8, etc. Compared with the single-channel LAMP detector, it is more effective The space is utilized, the volume of the whole machine will not increase significantly, and no additional space will be taken up. On the premise of adding multiple detection channels, the volume of the detector is still similar to that of a single-channel detector, with a more compact structure, optimized space occupation, small size, simple operation, convenient use, and cost savings.

The above-described embodiment is only to illustrate the technical concept and characteristics of the present invention. It is a preferred embodiment. Its purpose is that those familiar with this technology can understand the content of the present invention and implement it accordingly, and cannot limit the scope of the present invention. protected range. All equivalent changes or modifications made according to the principles of the present invention shall fall within the protection scope of the present invention.

Claims

A multi-channel LAMP detector is characterized in that, comprising:

The shell is provided with a chip port for inserting the microfluidic chip;

A chip chamber, which is arranged in the housing, and the chip chamber has a plurality of chip slots for accommodating microfluidic chips, and the plurality of chip slots are arranged side by side along the front and rear directions, and each of the chip slots Respectively have notches opposite to the chip ports;

a heating component, which is used for constant temperature heating of the microfluidic chip in the chip slot;

A mechanical arm mechanism, which includes a plurality of mechanical arms capable of engaging with the pistons of the microfluidic chips in the chip chamber, each of the chip slots corresponds to at least one of the mechanical arms, and each of the mechanical arms can move along the left and right is arranged in the casing to move in direction and is located on the right side of the chip compartment; and

An optical detection mechanism, which can be moved in the left-right direction and the front-rear direction, is arranged in the housing, and the optical detection mechanism is located below the chip compartment;

Wherein, the optical detection mechanism has a plurality of detection positions, each of the chip slots has a plurality of detection positions arranged along the left and right directions, and each of the detection positions of each of the chip slots corresponds to one The detection position.
The multi-channel LAMP detector according to claim 1, wherein the multi-channel LAMP detector also includes:

a door, which is used to close the chip opening, and the door is movably arranged on the inner wall of the housing;

The first driving mechanism is used to drive the door to move.
The multi-channel LAMP detector according to claim 2, wherein a first guide rail is provided on the inner wall of the housing, the door is movably arranged on the first guide rail, and the first drive The mechanism includes a first motor and a first screw driven by the first motor, and the first screw is connected with the door.
The multi-channel LAMP detector according to claim 1, wherein the multi-channel LAMP detector further comprises a chip cover, and the chip cover is arranged between the inner wall of the housing surrounding the chip port and the chip chamber between and between the chip sockets.
The multi-channel LAMP detector according to claim 1, wherein the multi-channel LAMP detector also includes a code scanner for reading the barcode on the microfluidic chip, and the code scanner is located at the The casing is provided with a scanning window, and the scanning part of the code scanner is located in the scanning window or facing the scanning window.
The multi-channel LAMP detector according to claim 5, wherein the multi-channel LAMP detector also includes a controller and a touch screen, and the controller is connected to the scanner and the touch screen respectively. The display screen is electrically connected, and the touch display screen has a first display state, and in the first display state, the touch display screen has a code scanning input button.
The multi-channel LAMP detector according to claim 1, wherein each of the chip slots corresponds to at least one of the heating components, and the heating components are arranged on the side walls of the chip slots.
The multi-channel LAMP detector according to claim 1, wherein the mechanical arm mechanism includes a bracket arranged on the inner wall of the housing, a lower mounting plate that can be arranged on the bracket along the left and right directions, and a lower mounting plate that can be arranged along the inner wall of the housing. The upper mounting plate arranged on the bracket to move left and right, the upper mounting plate is located above the lower mounting plate, a plurality of first mechanical arms are arranged on the upper mounting plate, and the lower mounting plate A plurality of second mechanical arms are provided, each of the chip sockets corresponds to at least one of the first mechanical arms and at least one of the second mechanical arms, and at the initial position, the second mechanical arms are located at the the bottom left of the first mechanical arm.
The multi-channel LAMP detector according to claim 8, wherein the first mechanical arm and/or the second mechanical arm respectively have engagement grooves for the piston of the microfluidic chip to snap into, and the The engagement groove has an upwardly facing notch.
The LAMP detector according to claim 8, wherein the mechanical arm mechanism further comprises a second drive mechanism for driving the upper mounting plate to move left and right, and a second drive mechanism for driving the lower mounting plate to move left and right. The third driving mechanism, the bracket is provided with a second guide rail and a third guide rail extending in the left and right direction, the upper mounting plate is arranged on the second guide rail so as to be movable in the left and right direction, and the lower mounting plate can be It is arranged on the third guide rail so as to move in the left and right directions, the second driving mechanism includes a second motor and a second screw driven by the second motor, the second screw and the upper mounting The third driving mechanism includes a third motor and a third screw driven by the third motor, and the third screw is connected to the lower mounting plate.
The LAMP detector according to claim 8, wherein the mechanical arm mechanism further comprises a first photoelectric switch for detecting whether the first mechanical arm reaches its initial position, and a first photoelectric switch for detecting whether the second mechanical arm reaches its initial position. Whether the mechanical arm has reached its initial position or not is the second photoelectric switch.
The LAMP detector according to claim 8, wherein the bracket has a blocking portion for preventing the upper mounting plate and the lower mounting plate from moving rightward beyond their set travel.
The multi-channel LAMP detector according to claim 1, wherein the multi-channel LAMP detector comprises a base arranged on the inner wall of the housing, and the base has an X-guiding rail extending in the left-right direction; The multi-channel LAMP detector also includes a mounting block that can move along the left and right directions and is arranged on the X-guiding rail, and the mounting block has a Y-guiding rail extending along the front-to-back direction; the optical detection mechanism can move along the front-to-back direction The ground is set on the Y guide rail.
The multi-channel LAMP detector according to claim 9, wherein the multi-channel LAMP detector further comprises a fourth drive mechanism for driving the mounting block to move along the X guide rail, for driving the The fifth driving mechanism for the optical detection mechanism to move along the Y guide rail, the X-direction start detection switch for detecting whether the installation block reaches its starting point, and the X-direction detection switch for detecting whether the installation block reaches its end point An end detection switch, a Y-direction start detection switch for detecting whether the optical detection mechanism reaches its starting point, and a Y-direction end detection switch for detecting whether the optical detection mechanism reaches its end point, and the X-direction start detection The switch and the X-direction end detection switch are respectively close to the two ends of the X-direction rail, and the Y-direction start detection switch and the Y-direction end detection switch are respectively close to the two ends of the Y-direction rail.
The multi-channel LAMP detector according to claim 1, wherein the multi-channel LAMP detector also includes a main PCB board, the housing is provided with a mounting frame, and the mounting frame is provided with a guide chute, The edge portion of the main PCB board is inserted into the guide chute.