CN217093572U - Temperature control assembly and rapid PCR fluorescent quantitative detection analyzer with same - Google Patents
Temperature control assembly and rapid PCR fluorescent quantitative detection analyzer with same Download PDFInfo
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- CN217093572U CN217093572U CN202220398194.5U CN202220398194U CN217093572U CN 217093572 U CN217093572 U CN 217093572U CN 202220398194 U CN202220398194 U CN 202220398194U CN 217093572 U CN217093572 U CN 217093572U
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Abstract
The utility model provides a temperature control assembly and have its quick PCR fluorescence quantitative determination analysis appearance, temperature control assembly wherein, include: a heating module capable of exchanging heat with a reagent in a reagent tube, the heating module having a high temperature portion and a low temperature portion; and a first driving device capable of driving the heating module to switch between a first position and a second position, wherein the first position is a position where the high temperature portion exchanges heat with the reagent in the reagent tube, and the second position is a position where the low temperature portion exchanges heat with the reagent in the reagent tube. The utility model discloses, through a drive arrangement can drive fast the high temperature portion and the low temperature portion of heating module with the switching of the heat exchange of reagent pipe can show and shorten equipment intensification, cooling time, improves detection efficiency.
Description
Technical Field
The utility model belongs to the technical field of molecular biology detects, concretely relates to temperature control component and have its quick PCR fluorescence quantitative determination analysis appearance.
Background
Polymerase Chain Reaction (PCR) is a molecular biology technique used to amplify a specific DNA fragment. The amplification of DNA consists of three basic reaction steps of denaturation, annealing and extension. The trace amount of DNA was greatly increased by several tens of repetitive cycles. Because the PCR amplification needs dozens of cycles, and each cycle needs temperature rise and drop, the temperature change speed can determine the detection efficiency of the whole PCR. Particularly, with the development and development progress of enzyme technology, the annealing time can be shortened by fast enzyme, and the temperature-variable technology becomes a bottleneck influencing the rapid progress of PCR (polymerase chain reaction) because the high-temperature-resistant enzyme does not need denaturation and retention time. The PCR is generally carried out by controlling the temperature and heating time of each reaction stage depending on the PCR instrument. In general, the method adopts a two-step method for proving the quantitative determination, so as to reduce the temperature rise and fall process once and improve the reaction speed.
The real-time fluorescent quantitative PCR detection device in the current market basically adopts a Peltier to control the temperature of PCR amplification reaction, the Peltier has the functions of heating and refrigerating, and the Peltier function is utilized to circularly work of heating and cooling in the PCR amplification reaction process. The method needs about 2 minutes for completing one cycle, and the experiment can be completed within 1-2 hours. Generally, the detection efficiency is improved by increasing the power of the heating and heat sink, or making the PCR tube small, such as making the PCR tube into a capillary tube.
However, by increasing the power of the heating and heat sink, the effect is not obvious, the temperature control precision is also poor, and the sample adding is inconvenient because the PCR tube is made small.
SUMMERY OF THE UTILITY MODEL
Therefore, the to-be-solved technical problem of the utility model is to provide a temperature control component and have its quick PCR fluorescence quantitative determination analysis appearance can show and shorten equipment intensification, cooling time, improve detection efficiency.
In order to solve the above problem, the utility model provides a temperature control assembly, include:
a heating module capable of exchanging heat with a reagent in a reagent tube, the heating module having a high temperature portion and a low temperature portion;
and a first driving device capable of driving the heating module to switch between a first position and a second position, wherein the first position is a position where the high temperature portion exchanges heat with the reagent in the reagent tube, and the second position is a position where the low temperature portion exchanges heat with the reagent in the reagent tube.
In some embodiments, the temperature control assembly further comprises:
the side vertical parts are provided with two side vertical parts which are opposite to each other and are arranged at intervals;
the heating block pressing block is erected at the tops of the two side vertical pieces;
the heating block is provided with a placing structure for accommodating the reagent tube, and the heating block pressing block can press down the heating block and can form heat exchange with the heating block;
when the first driving device is in the first position, the high-temperature portion is in contact with the heating block, and when the first driving device is in the second position, the low-temperature portion is in contact with the heating block.
In some embodiments, at least one side of the high temperature part away from the heating block is provided with an elastic member capable of exerting an elastic force on the high temperature part toward the heating block side; and/or the presence of a gas in the gas,
at least one side of the low-temperature part, which is far away from the heating block, is provided with an elastic component, and the elastic component can apply elastic force towards one side of the heating block on the low-temperature part.
In some embodiments, the high temperature portion comprises a peltier or heating film; alternatively, the first and second electrodes may be,
the low temperature part includes a peltier and a heat sink.
In some embodiments, the heating module has a temperature sensing element and/or an overheat protector thereon; alternatively, the first driving means comprises a first lead screw motor.
In some embodiments, the first preset temperature of the high temperature portion is not lower than the first target temperature of the reagent tube, and the second preset temperature of the low temperature portion is not higher than the second target temperature of the reagent tube.
The utility model also provides a quick PCR fluorescence quantitative determination analysis appearance, including at least one as foretell control by temperature change part.
In some embodiments, the rapid PCR fluorogenic quantitative detection analyzer further comprises:
the temperature control assembly is assembled on the mounting bottom plate, and a linear guide rail is arranged between the bottom of the heating module and the mounting bottom plate.
In some embodiments, the rapid PCR fluorogenic quantitative detection analyzer further comprises:
and the optical detection assembly is assembled on the mounting bottom plate, a heat insulation plate is arranged between the optical detection assembly and the temperature control assembly, and the heat insulation plate can at least partially block the heat of the high-temperature part from being transferred to one side of the optical detection assembly.
In some embodiments, the optical detection assembly comprises: an optical detection unit;
the second driving device comprises a second screw motor and can drive the optical detection part to reciprocate along a straight line;
and the optical fiber installation module is used for connecting one end of an optical fiber so as to realize the fluorescence excitation and detection of the optical detection part on the corresponding reagent in the reagent tube through the optical fiber.
The utility model provides a pair of temperature control assembly and have its quick PCR fluorescence quantitative determination analysis appearance, through a drive arrangement can drive fast heating module's high temperature portion and low temperature portion with the switching of the heat exchange of reagent pipe can show ground and shorten equipment intensification, cooling time, improves detection efficiency.
Drawings
Fig. 1 is a schematic top view of a temperature control assembly according to an embodiment of the present invention;
fig. 2 is a schematic side view of a temperature control assembly according to an embodiment of the present invention;
FIG. 3 is a schematic diagram of the internal structure of a rapid PCR fluorescence quantitative detection analyzer according to another embodiment of the present invention (omitting the outer case, etc.);
fig. 4 is a schematic structural diagram of the optical detection assembly in fig. 3.
The reference numerals are represented as:
11. a side stand member; 12. pressing the heating block; 131. a high-temperature section; 132. a low temperature section; 133. peltier; 134. a heat sink; 14. a heating block; 151. a first lead screw motor; 152. a linear guide rail; 100. a reagent tube; 201. mounting a bottom plate; 300. an optical detection assembly; 301. an optical detection unit; 302. a second lead screw motor; 303. an optical fiber installation module; 400. an insulating panel.
Detailed Description
Referring to fig. 1 to 4, according to an embodiment of the present invention, there is provided a temperature control assembly including: a heat block capable of exchanging heat with the reagent in the reagent tube 100, the heat block having a high temperature part 131 and a low temperature part 132; and a first driving means capable of driving the heating module to switch between a first position where the high temperature part 131 exchanges heat with the reagent in the reagent tube 100 and a second position where the low temperature part 132 exchanges heat with the reagent in the reagent tube 100. In this embodiment, the first driving device can rapidly drive the switching of the heat exchange between the high-temperature portion 131 and the low-temperature portion 132 of the heating module and the reagent tube 100, so that the time for heating and cooling the device can be significantly shortened, and the detection efficiency can be improved.
As a specific implementation manner, the temperature control assembly further includes: the side vertical parts 11 are provided with two, and the two side vertical parts 11 are opposite to each other and are arranged at intervals; the heating block pressing block 12 is erected at the tops of the two side vertical pieces 11; a heating block 14, on which a placing structure for accommodating the reagent tube 100 is configured, the heating block pressing block 12 can press down the heating block 14 and the heating block can form heat exchange with the heating block 14; when the first driving means is in the first position, the high temperature part 131 is in contact with the heating block 14, and when the first driving means is in the second position, the low temperature part 132 is in contact with the heating block 14. In the technical scheme, the heating block 14 is adopted to conduct the heat (or cold) of the heating block to the reagent tube 100, so that the heat exchange is more sufficient and efficient. The placing structures are, for example, groove structures, which can form a complete surrounding for the bottom of the reagent tube 100 and the tube wall with the reagent, obviously improving the efficiency of heat exchange.
In some embodiments, at least a side of the high temperature part 131 away from the heating block 14 has an elastic member capable of exerting an elastic force on the high temperature part 131 toward the heating block 14 side; and/or at least one side of the low-temperature part 132, which is far away from the heating block 14, is provided with an elastic component, the elastic component can apply an elastic force to the low-temperature part 132, which faces one side of the heating block 14, and can ensure that the high-temperature part 131 and the low-temperature part 132 are tightly attached to a heated and cooled target after moving to positions (i.e. after switching positions), so that the heat transfer efficiency is further improved.
As a specific example, the high temperature part 131 includes a peltier 133 or a heating film; alternatively, the low temperature part 132 includes the peltier element 133 and the heat sink 134 (for heat radiation), and a heating element having a planar structure is used, so that heating (or cooling) can be more uniform.
The heating module is provided with a temperature detection element for monitoring the temperature of the module so as to be convenient for temperature adjustment; and/or, an overheat protector is arranged on the heating module, specifically, the overheat protector is arranged for the high-temperature part 131 and is used for cutting off the heating power supply under the condition of overhigh temperature, stopping heating and improving the safety of the assembly.
The first driving device includes a first lead screw motor 151, which can convert the rotary motion of the motor into linear motion, simplifying the assembly structure.
In some embodiments, the first preset temperature of the high temperature part 131 is not lower than the first target temperature of the reagent tube 100, and the second preset temperature of the low temperature part 132 is not higher than the second target temperature of the reagent tube 100, and specifically, the high temperature part 131 or the low temperature part 132 may be a constant temperature module or a non-constant temperature module. The set temperature (i.e., the first preset temperature) T1 of the high temperature part 131 is greater than or equal to a first target temperature Ta, and the set temperature T2 (i.e., the second preset temperature) of the low temperature part 132 is less than or equal to a second target temperature Tb. So can heat or lower the temperature more fast, if object temperature 60 ℃, it is 90 ℃ to need to heat the target temperature, heats faster with the module of 100 ℃ than with the heating of 90 ℃, and efficiency is higher. The same applies to cooling.
According to the embodiment of the utility model, still provide a quick PCR fluorescence quantitative determination analysis appearance, include at least one as foretell temperature control part. That is, the analyzer may have one temperature control unit, and a plurality of temperature control units may be provided. Specifically, the rapid PCR fluorescence quantitative detection analyzer further comprises: the temperature control assembly is assembled on the mounting base plate 201, and a linear guide rail 152 is arranged between the bottom of the heating module and the mounting base plate 201, so that the reliability, stability and smoothness of position switching of the heating module can be guaranteed.
In some embodiments, the rapid PCR fluorogenic quantitative detection analyzer further comprises: an optical detection assembly 300 assembled on the mounting base plate 201, a heat insulation plate 400 is disposed between the optical detection assembly 300 and the temperature control assembly, the heat insulation plate 400 can at least partially block the heat of the high temperature portion 131 from being transferred to one side of the optical detection assembly 300, and at this time, a control board (not shown in the figure) of the analyzer is disposed in an area between the heat insulation plate 400 and the optical detection assembly 300, so as to effectively prevent the heat of the high temperature portion 131 from causing the temperature of the control board to be too high, and ensure the stability and reliability of the control. It will be appreciated that the control board is used to control the power supply of the apparatus, the heating power supply, the processing of information data, etc.
In some embodiments, the optical detection assembly 300 includes: an optical detection portion 301, as will be appreciated, for detecting a quantitative detection of fluorescence, having LED light therein; a second driving means including a second screw motor 302 capable of driving the optical detection portion 301 to reciprocate in a straight line; and the optical fiber installation module 303 is configured to connect one end of an optical fiber to further realize fluorescence excitation and detection of the optical detection unit 301 on the corresponding reagent in the reagent tube 100 through the optical fiber. Specifically, one end of the optical fiber (not shown in the figure) is fixed on the optical fiber installation module 303, the other end of the optical fiber is fixed on one side of the reagent tube 100, the LED light in the optical detection portion 301 is transmitted into the reagent tube through the optical fiber, the fluorescence of the reagent is excited, and the excited fluorescence is transmitted to the detection chip on the optical detection portion 301 through the optical fiber and is subjected to data processing, so that the quantitative detection of the fluorescence is realized.
It is readily understood by a person skilled in the art that the advantageous ways described above can be freely combined, superimposed without conflict.
The above description is only exemplary of the present invention and should not be construed as limiting the present invention, and any modifications, equivalents and improvements made within the spirit and principles of the present invention are intended to be included within the scope of the present invention. The above is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.
Claims (10)
1. A temperature control assembly, comprising:
a heating module capable of exchanging heat with the reagent in the reagent tube (100), the heating module having a high-temperature part (131) and a low-temperature part (132);
and a first driving device capable of driving the heating module to switch between a first position where the high temperature part (131) exchanges heat with the reagent in the reagent tube (100) and a second position where the low temperature part (132) exchanges heat with the reagent in the reagent tube (100).
2. The temperature control assembly of claim 1, further comprising:
the side vertical parts (11) are provided with two, and the two side vertical parts (11) are opposite to each other and are arranged at intervals;
the heating block pressing block (12) is erected at the tops of the two side vertical pieces (11);
a heating block (14) which is provided with a placing structure for accommodating the reagent tube (100), wherein the heating block pressing block (12) can press down the heating block (14) and can exchange heat with the heating block (14);
when the first drive means is in the first position, contact is made between the high temperature portion (131) and the heating block (14), and when the first drive means is in the second position, contact is made between the low temperature portion (132) and the heating block (14).
3. The temperature control assembly of claim 2,
at least one side of the high-temperature part (131) far away from the heating block (14) is provided with an elastic component which can apply elastic force to the high-temperature part (131) towards one side of the heating block (14); and/or the presence of a gas in the gas,
at least one side of the low-temperature part (132) far away from the heating block (14) is provided with an elastic component which can apply elastic force to the low-temperature part (132) towards one side of the heating block (14).
4. The temperature control assembly of claim 1,
the high temperature part (131) includes a Peltier (133) or a heating film; alternatively, the first and second electrodes may be,
the low-temperature part (132) includes a Peltier (133) and a heat sink (134).
5. The temperature control assembly of claim 1,
the heating module is provided with a temperature detection element and/or an overheat protector; alternatively, the first and second electrodes may be,
the first driving means includes a first lead screw motor (151).
6. The temperature control assembly of claim 1,
the first preset temperature of the high-temperature part (131) is not lower than the first target temperature of the reagent tube (100), and the second preset temperature of the low-temperature part (132) is not higher than the second target temperature of the reagent tube (100).
7. A rapid PCR fluorometric quantitative analyzer comprising at least one temperature control assembly according to any of claims 1 to 6.
8. The rapid PCR fluorometric quantitative detection analyzer of claim 7, further comprising:
the temperature control module is assembled on the mounting base plate (201), and a linear guide rail (152) is arranged between the bottom of the heating module and the mounting base plate (201).
9. The rapid PCR fluorometric quantitative detection analyzer of claim 8, further comprising:
the optical detection assembly (300) is assembled on the installation bottom plate (201), a heat insulation plate (400) is arranged between the optical detection assembly (300) and the temperature control assembly, and the heat insulation plate (400) can at least partially block the heat of the high-temperature part (131) from being transferred to one side of the optical detection assembly (300).
10. The rapid PCR fluorometric quantitative detection analyzer of claim 9, wherein the optical detection assembly (300) comprises:
an optical detection unit (301);
a second driving device including a second screw motor (302) capable of driving the optical detection unit (301) to reciprocate linearly;
and the optical fiber installation module (303) is used for connecting one end of an optical fiber so as to realize fluorescence excitation and detection of the optical detection part (301) on the corresponding reagent in the reagent tube (100) through the optical fiber.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220398194.5U CN217093572U (en) | 2022-02-26 | 2022-02-26 | Temperature control assembly and rapid PCR fluorescent quantitative detection analyzer with same |
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| CN202220398194.5U CN217093572U (en) | 2022-02-26 | 2022-02-26 | Temperature control assembly and rapid PCR fluorescent quantitative detection analyzer with same |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114433267A (en) * | 2022-02-26 | 2022-05-06 | 江苏宏微特斯生物科技有限公司 | Temperature control assembly and rapid PCR fluorescent quantitative detection analyzer with same |
| CN117402721A (en) * | 2023-11-03 | 2024-01-16 | 苏州思迈德生物科技有限公司 | Detection device and detection method for multicolor fluorescence detection |
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2022
- 2022-02-26 CN CN202220398194.5U patent/CN217093572U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114433267A (en) * | 2022-02-26 | 2022-05-06 | 江苏宏微特斯生物科技有限公司 | Temperature control assembly and rapid PCR fluorescent quantitative detection analyzer with same |
| CN117402721A (en) * | 2023-11-03 | 2024-01-16 | 苏州思迈德生物科技有限公司 | Detection device and detection method for multicolor fluorescence detection |
| CN117402721B (en) * | 2023-11-03 | 2024-04-19 | 苏州思迈德生物科技有限公司 | Detection device and detection method for multicolor fluorescence detection |
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Address after: Room 1401, building 1, No. 188, Fuchunjiang Road, high tech Zone, Suzhou, Jiangsu 215151 Patentee after: Hongwei TEX (Suzhou) Bioengineering Co.,Ltd. Patentee after: JIANGSU MACRO&MICRO-TEST MED-TECH Co.,Ltd. Address before: Room 1401, building 1, No. 188, Fuchunjiang Road, high tech Zone, Suzhou, Jiangsu 215151 Patentee before: Jiangsu hongweites Biotechnology Co.,Ltd. Patentee before: JIANGSU MACRO&MICRO-TEST MED-TECH Co.,Ltd. |