CN220467974U

CN220467974U - Reaction tank device and PCR instrument

Info

Publication number: CN220467974U
Application number: CN202321972420.7U
Authority: CN
Inventors: 李小杰; 陈虹旭; 高元鹏; 梁锁锋; 潘良斌
Original assignee: Chengdu Boao Jingxin Biotechnology Co ltd; Sichuan National Academy Of High Performance Medical Devices Co ltd; Beijing Capitalbio Technology Co ltd
Current assignee: Chengdu Boao Jingxin Biotechnology Co ltd; Sichuan National Academy Of High Performance Medical Devices Co ltd; Beijing Capitalbio Technology Co ltd
Priority date: 2023-07-25
Filing date: 2023-07-25
Publication date: 2024-02-09
Anticipated expiration: 2033-07-25

Abstract

The utility model discloses a reaction tank device and a PCR instrument, wherein the reaction tank device comprises: a radiator (1); a heat radiation fan (2), wherein the heat radiation fan (2) can drive air flow to pass through the radiator (1) and exchange heat with the radiator; the reaction tank assembly (3), the reaction tank assembly (3) comprises a reaction tank (32) and a heating and refrigerating piece (31) for heating and cooling the reaction tank (32), the heating and refrigerating piece (31) and the reaction tank (32) are of an integrated structure, and the heating and refrigerating piece (31) is in heat conduction contact with the radiator (1); and a temperature detection structure for detecting the temperature of the reaction tank (32). The reaction tank device provided by the utility model improves the temperature rising and falling rate.

Description

Reaction tank device and PCR instrument

Technical Field

The utility model relates to the technical field of biological detection equipment, in particular to a reaction tank device and a PCR instrument.

Background

The real-time fluorescent quantitative polymerase chain reaction (Polymerase Chain Reaction, PCR) technology can realize quantitative analysis of the DNA template, and has important significance for molecular biology research, medical research and the like.

During the PCR gene amplification process, the temperature of the reaction tank can undergo repeated rapid changes, and one experiment can usually be completed through a plurality of temperature cycles, so that the temperature rise and fall rate of the reaction tank determines the experiment efficiency of the PCR instrument.

The existing reaction tank is installed together with the semiconductor heating and refrigerating piece through the heat conducting medium, and the reaction tank and the semiconductor heating and refrigerating piece are independent individuals, so that when heat is transferred, even if the heat conducting medium exists, certain heat loss can be caused, and the temperature rising and falling rate of the reaction tank is influenced.

Disclosure of Invention

In view of the above, the present utility model provides a reaction tank apparatus to increase the temperature rising and falling rate. The utility model also provides a PCR instrument.

In order to achieve the above purpose, the present utility model provides the following technical solutions:

a reaction cell apparatus, comprising:

a heat sink;

the heat dissipation fan can drive air flow to pass through the radiator and exchange heat with the radiator;

the reaction tank assembly comprises a reaction tank and a heating and refrigerating piece for heating and cooling the reaction tank, the heating and refrigerating piece and the reaction tank are of an integrated structure, and the heating and refrigerating piece is in heat conduction contact with the radiator;

and the temperature detection structure is used for detecting the temperature of the reaction tank.

Optionally, in the reaction tank device, a heat conducting medium for transferring heat is arranged between the reaction tank assembly and the radiator.

Optionally, in the reaction tank device, a plurality of heat dissipation fins are provided on a lower surface of the heat sink;

the heat radiation fan can drive air flow to pass through the heat radiation fins and make heat exchange contact with the surfaces of the heat radiation fins;

one side of the heating and refrigerating piece, which is opposite to the reaction tank, is in heat conduction contact with the upper surface of the radiator;

the upper surface of the radiator is opposite to the lower surface of the radiator.

Optionally, in the reaction tank device, the number of the cooling fans is 2N, and N is more than or equal to 1;

n radiating fans form a first radiating fan group, and N radiating fans form a second radiating fan group;

the first group of radiating fan groups and the second group of radiating fan groups are respectively arranged at two ends of the radiator and are arranged along the extending direction of the radiating fins;

the direction of the air flow driven by the first group of cooling fan groups and the second group of cooling fan groups is the same.

Optionally, in the reaction tank device, the reaction tank includes a reaction tank main body and a plurality of reaction tank columns disposed on an upper surface of the reaction tank main body, and the upper surface of the reaction tank main body is a surface of the reaction tank main body facing away from the heating and refrigerating member;

the reaction tank upright post is provided with an opening for bearing a bearing container used for bearing experiments.

Optionally, in the reaction tank device, a temperature measuring hole without the reaction tank upright post is formed in the center of the reaction tank main body;

the temperature probe of the temperature detection structure is arranged in the temperature measuring hole.

Optionally, the reaction tank device further comprises an optical fiber bundle, wherein the optical fiber bundle is used for detecting the illumination intensity change of the liquid in the bearing container in the reaction tank upright post.

Optionally, in the reaction tank device, the reaction tank upright post is provided with at least two optical fiber holes penetrating through the inner wall and the outer wall of the reaction tank upright post;

at least one of the fiber holes is used for mounting the optical fiber bundle of incident light, and at least one of the fiber holes is used for mounting the optical fiber bundle of receiving light.

Optionally, the reaction tank device further comprises a fixing cover for fixedly connecting the reaction tank assembly and the radiator;

the fixed cover is provided with a hollowed-out avoiding structure for the upright post of the reaction tank to penetrate out and a solid frame structure for positioning the edge of the reaction tank main body and the edge of the heating and refrigerating piece;

one surface of the solid frame structure, which faces the radiator, is abutted to the upper surface of the reaction tank main body, and the solid frame structure is fixedly connected with the radiator.

Optionally, in the reaction tank device, the reaction tank further comprises a heat preservation medium surrounding the periphery of the reaction tank upright post, and one surface of the heat preservation medium, which faces the radiator, is in contact with the fixed cover.

Optionally, the reaction tank device further comprises a digital board, wherein the digital board is positioned on one surface of the heat preservation medium, which is opposite to the radiator;

the digital board is provided with a plurality of through holes which are in one-to-one correspondence with the reaction tank upright posts and can be used for exposing the openings of the reaction tank upright posts;

the upper surface of the digital board is provided with digital marks corresponding to the through holes one by one, and the upper surface of the digital board is the surface of the digital board, which is opposite to the radiator.

Optionally, in the reaction tank device, the reaction tank further includes a connecting piece, one end of the connecting piece is connected with the digital board, and the other end of the connecting piece is connected with the radiator.

The utility model also provides a PCR instrument, which comprises a reaction tank device, and is characterized in that the reaction tank device is the reaction tank device.

According to the technical scheme, the temperature detection structure is used for detecting the temperature of the reaction tank so as to obtain the temperature of the reaction tank; the radiator fan can drive the air flow to pass through the radiator, the radiator fan can be started to drive the air flow to radiate the radiator, and the heating and refrigerating piece is in heat conduction contact with the radiator, so that the temperature adjusting effect of the heating and refrigerating piece can be ensured. And the heating and refrigerating piece is used for heating and cooling the reaction tank, and the heating and refrigerating piece and the reaction tank are of an integrated structure, so that the refrigerating effect of the heating and refrigerating piece can directly act on the reaction tank, the heat transfer efficiency is improved, the heat loss transferred between the heating and refrigerating piece and the reaction tank is reduced, and the heating and cooling rate is further improved.

The embodiment of the utility model also provides a PCR instrument, which comprises a reaction tank device, wherein the reaction tank device is any one of the reaction tank devices. Since the reaction cell apparatus has the above technical effects, the PCR apparatus having the reaction cell apparatus should have the same technical effects, and will not be described in detail herein.

Drawings

In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic structural diagram of a reaction tank device according to an embodiment of the present utility model;

FIG. 2 is an exploded view of a reaction tank apparatus according to an embodiment of the present utility model;

FIG. 3 is an exploded view of a reaction cell assembly according to an embodiment of the present utility model.

Wherein,

the heat radiator comprises a radiator body 1, heat radiating fins 11, a heat radiating fan 2, a reaction tank assembly 3, a heating and refrigerating part 31, a reaction tank 32, a reaction Chi Lizhu-321, an optical fiber hole 3211, a temperature measuring hole 322, a fixed cover 4, a temperature probe 5, an optical fiber bundle 6, a heat preservation medium 7, a digital board 8 and a connecting part 9.

Detailed Description

The utility model discloses a reaction tank device for improving the temperature rising and falling rate. The utility model also provides a PCR instrument.

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

As shown in fig. 1, an embodiment of the present utility model provides a reaction tank device, which includes a radiator 1, a cooling fan 2, a reaction tank assembly 3, and a temperature detection structure.

Wherein, the radiator fan 2 can drive the air flow to pass through the radiator 1 and exchange heat with the radiator; the reaction tank assembly 3 comprises a heating and refrigerating piece 31 and a reaction tank 32, wherein the heating and refrigerating piece 31 is used for heating and cooling the reaction tank 32, the heating and refrigerating piece 31 and the reaction tank 32 are of an integrated structure, and the heating and refrigerating piece 31 is in heat conduction contact with the radiator 1; the temperature detecting structure is used for detecting the temperature of the reaction tank 32.

The reaction tank device provided by the embodiment of the utility model is characterized in that the temperature detection structure is used for detecting the temperature of the reaction tank 32 so as to obtain the temperature of the reaction tank 32; the radiator fan 2 can drive the air flow to pass through the radiator 1, the air flow can be driven to radiate the radiator 1 by the starting of the radiator fan 2, and the heating and refrigerating piece 31 is in heat conduction contact with the radiator 1, so that the temperature adjusting effect of the heating and refrigerating piece 31 can be ensured. And, heating and refrigerating piece 31 is used for carrying out the temperature rise and drop to reaction tank 32, and heating and refrigerating piece 31 and reaction tank 32 are integrated into one piece structure for the refrigeration effect of heating and refrigerating piece 31 can directly act on reaction tank 32, has increased heat transfer efficiency, has reduced the heat loss of transmission between heating and refrigerating piece 31 and reaction tank 32, and then has improved the temperature rise and drop rate.

And by improving the temperature rise and fall rate of the reaction tank device, the time required by the PCR instrument in the experimental process is saved. The heating and refrigerating piece 31 and the reaction tank 32 are of an integrated structure, so that the number of parts in the assembly process of the reaction tank device can be reduced, the reaction tank assembly 3 is convenient to install, the assembly of the reaction tank device is simplified, and the structural complexity of the reaction tank device is reduced.

It will be appreciated that the heating and cooling element 31 and the reaction tank 32 may be manufactured separately and then welded together to form a unitary structure. Other ways may be used to make the heating and cooling element 31 and the reaction tank 32 an integral structure, such as integral injection molding.

Among them, the heating/cooling element 31 is preferably a semiconductor cooling plate, and the semiconductor cooling plate can be heated and cooled by applying voltages with opposite polarities to both ends of the semiconductor cooling plate.

The material of the radiator 1 can be made of aluminum or other metals with high heat conduction speed.

Of course, other devices may be selected as the heating and cooling element 31, and only the heating and cooling element 31 may be required to perform heating and cooling.

Further, a heat transfer medium for transferring heat is provided between the reaction tank assembly 3 and the heat sink 1. Wherein, the heat-conducting medium is evenly smeared between the reaction tank component 3 and the radiator 1, so that the heat transfer efficiency can be effectively increased. Wherein the heat conducting medium is a medium for transferring heat, such as, but not limited to, heat conducting silicone grease.

Preferably, the lower surface of the heat sink 1 has a plurality of heat radiation fins 11; the heat radiation fan 2 can drive air flow to pass through the heat radiation fins 11 and make heat exchange contact with the surfaces of the heat radiation fins 11; one side of the heating and refrigerating piece 31, which is away from the reaction tank 32, is in heat conduction contact with the upper surface of the radiator 1; the upper surface of the heat sink 1 is disposed opposite to the lower surface thereof. Through the arrangement, on the basis of increasing the heat exchange area of the radiator 1, the heat exchange area of the heating and refrigerating piece 31 and the radiator 1 is increased, and then the heat exchange effect of the radiator 1 on the heating and refrigerating piece 31 is ensured.

Further, the number of the radiating fans 2 is 2N, and N is more than or equal to 1; the N radiator fans 2 form a first group of radiator fan groups, and the N radiator fans 2 form a second group of radiator fan groups; the first group of cooling fans and the second group of cooling fans are respectively arranged at two ends of the radiator 1 and are arranged along the extending direction of the cooling fins 11; the direction of the air flow driven by the first group of cooling fan groups and the second group of cooling fan groups is the same. The first group of cooling fan groups and the second group of cooling fan groups can drive air flow to flow through the radiator 1 in the same flow direction, so that air flow in a gap between two adjacent cooling fins 11 of the radiator 1 is effectively quickened, and the heat dissipation efficiency is increased.

That is, one of the first and second sets of cooling fans may blow fresh air from outside between the cooling fins 11, and the other of the first and second sets of cooling fans may draw high-temperature air between the cooling fins 11 out of the radiator 1 and exhaust the high-temperature air into the outside air, thereby realizing the internal and external exchange of air.

In the reaction tank device provided by the embodiment of the utility model, the reaction tank 32 comprises a reaction tank main body and a plurality of reactions Chi Lizhu 321 arranged on the upper surface of the reaction tank main body, wherein the upper surface of the reaction tank main body is the surface of the reaction tank main body facing away from the heating and refrigerating piece 31; the reaction cell post 321 has an opening for carrying a carrying container for experiments. Wherein, the reaction tank upright post 321 can be pressed on the upper surface of the reaction tank main body by adopting a pressing process, and can also be integrally processed with the reaction tank main body.

The specification of the carrying container needs to be matched with parameters such as the number, the interval and the like of the reaction tank stand columns 321 on the reaction tank 32. As shown in fig. 1, the number of the reaction cell pillars 321 is 16 and arranged in a matrix, and the 16 reaction cell pillars 321 are divided into two rows of 8 reaction cell pillars 321 each. That is, the above-mentioned carrying container used for carrying experiments may be eight-row tubes, or may be containers of other specifications, which are not described one by one and are all within the scope of protection.

Further, the center of the reaction tank body is a temperature measuring hole 322 without a reaction tank upright post 321, and a temperature probe 5 of the temperature detecting structure is arranged in the temperature measuring hole 322. Wherein, the temperature measuring hole 322 can be a cylindrical hole for placing the temperature probe 5, and the cylindrical hole can be smeared with a heat conducting medium, thereby increasing the accuracy of the temperature measurement of the detection probe 5. The heat conducting medium may be heat conducting silicone grease, or may be other heat conducting materials, and is not limited to heat conducting silicone grease. And high temperature resistant mediums such as high temperature resistant glue are smeared outside the temperature probe 5 for fixing the temperature probe 5. The center of the reaction tank body is provided with the temperature measuring hole 322, so that the overall average temperature of the reaction tank 32 can be accurately detected.

The reaction tank device provided by the embodiment of the utility model further comprises an optical fiber bundle 6, wherein the optical fiber bundle 6 is used for detecting the illumination intensity change of liquid in the drain pipes positioned in the reaction tank upright post 321.

The reaction cell post 321 has at least two fiber holes 3211 penetrating the inner and outer walls thereof; at least one fiber hole 3211 is used for mounting a fiber bundle 6 for incident light, and at least one fiber hole 3211 is used for mounting a fiber bundle 6 for receiving light. In this embodiment, the reaction cell post 321 has two fiber holes 3211 penetrating the inner wall and the outer wall thereof, that is, two fiber holes 3211 on the same reaction cell post 321, one for mounting the optical fiber bundle 6 for incident light and the other for mounting the optical fiber bundle 6 for receiving light.

The reaction tank device provided by the embodiment of the utility model further comprises a fixed cover 4 for fixedly connecting the reaction tank assembly 3 and the radiator 1; the fixed cover 4 is provided with a hollowed-out avoiding structure for the reaction tank upright post 321 to penetrate out and a solid frame structure for positioning the edge of the reaction tank main body and the edge of the heating and refrigerating piece 31; one surface of the solid frame structure, which faces the radiator 1, is abutted against the upper surface of the reaction tank main body, and the solid frame structure is fixedly connected with the radiator 1. The reaction tank stand 321 is exposed outside the fixed cover 4 through the hollowed-out avoidance structure and is connected with the radiator 1 through the solid frame structure of the fixed cover 4, so that the operation of fixing the reaction tank assembly 3 relative to the radiator 1 is realized.

In order to ensure the heat conducting effect of the heat conducting medium between the heat sink 1 and the reaction tank assembly 3, the heat conducting medium should not be coated too thick or too thin, otherwise the heat conducting efficiency is affected. And, at the time of pressing the reaction tank assembly 3, the fixing cover 4 is connected with the heat sink 1 through a plurality of connection members (e.g., screws). When the connecting pieces are screws, all screws should keep the tightening torque consistent, so that the reaction tank assembly 3 is horizontally arranged on the upper surface of the radiator 1, and the heat conduction efficiency is ensured.

Further, the reaction tank device further comprises a heat preservation medium 7 surrounding the periphery of the reaction tank upright post 321, and one surface of the heat preservation medium 7 facing the radiator 1 is contacted with the fixed cover 4. Through the above arrangement, the reaction tank stand 321 is kept warm. In this embodiment, the heat-insulating medium 7 is a frame structure, and the plurality of reaction tank pillars 321 are all located in the hollow area of the frame structure. The heat preservation medium 7 can be provided with a plurality of through hole structures in a number of the heat preservation medium 7 and the reaction tank stand columns 321 in a one-to-one correspondence manner, and the reaction tank stand columns 321 can penetrate through the through hole structures. The heat preservation medium 7 is made of foam, but is not limited to foam.

The reaction tank device provided by the embodiment of the utility model further comprises a digital board 8, wherein the digital board 8 is positioned on one surface of the heat preservation medium 7, which is opposite to the radiator 1; the digital plate 8 is provided with a plurality of through holes which are in one-to-one correspondence with the reaction tank upright posts 321 and can expose the openings of the reaction tank upright posts 321; the upper surface of the digital board 8 is provided with digital marks corresponding to the through holes one by one, and the upper surface of the digital board 8 is the surface of the digital board 8 facing away from the radiator 1. The function of the plurality of numerical identifiers is to label each reaction cell post 321.

In order to facilitate connection and improve the installation stability of the reaction tank assembly 3, the reaction tank device provided by the embodiment of the utility model further comprises a connecting piece 9, one end of the connecting piece 9 is connected with the digital board 8, and the other end of the connecting piece 9 is connected with the radiator 1. Wherein, the connecting piece 9 can be a hexagonal rod, and the operation of connecting the digital board 8 and the radiator 1 can be completed by rotating the connecting piece 9 by a human hand or a tool.

In the present specification, each embodiment is described in a progressive manner, and each embodiment is mainly described in a different point from other embodiments, and identical and similar parts between the embodiments are all enough to refer to each other.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present utility model. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the utility model. Thus, the present utility model is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A reaction cell apparatus, comprising:

a radiator (1);

a heat radiation fan (2), wherein the heat radiation fan (2) can drive air flow to pass through the radiator (1) and exchange heat with the radiator;

the reaction tank assembly (3), the reaction tank assembly (3) comprises a reaction tank (32) and a heating and refrigerating piece (31) for heating and cooling the reaction tank (32), the heating and refrigerating piece (31) and the reaction tank (32) are of an integrated structure, and the heating and refrigerating piece (31) is in heat conduction contact with the radiator (1);

and a temperature detection structure for detecting the temperature of the reaction tank (32).

2. A reaction cell arrangement according to claim 1, characterized in that a heat conducting medium for transferring heat is present between the reaction cell assembly (3) and the heat sink (1).

3. A reaction cell arrangement according to claim 1, characterized in that the lower surface of the heat sink (1) has a plurality of heat sink fins (11);

the heat radiation fan (2) can drive air flow to pass through the heat radiation fins (11) and is in heat exchange contact with the surfaces of the heat radiation fins (11);

one side of the heating and refrigerating piece (31) facing away from the reaction tank (32) is in heat conduction contact with the upper surface of the radiator (1);

the upper surface of the radiator (1) is arranged opposite to the lower surface thereof.

4. A reaction tank device according to claim 3, characterized in that the number of the cooling fans (2) is 2N, N being equal to or greater than 1;

n radiating fans (2) form a first radiating fan group, and N radiating fans (2) form a second radiating fan group;

the first group of radiating fan groups and the second group of radiating fan groups are respectively arranged at two ends of the radiator (1) and are arranged along the extending direction of the radiating fins (11);

5. The reaction tank device according to claim 1, wherein the reaction tank (32) comprises a reaction tank main body and a plurality of reactions Chi Lizhu (321) arranged on the upper surface of the reaction tank main body, and the upper surface of the reaction tank main body is a surface of the reaction tank main body facing away from the heating and refrigerating member (31);

the reaction Chi Lizhu (321) had an opening for a support vessel used for the support experiments.

6. The reaction cell apparatus according to claim 5, wherein the center of the reaction cell main body is a temperature measurement hole (322) in which the reaction Chi Lizhu (321) is not provided;

the temperature probe (5) of the temperature detection structure is arranged in the temperature measurement hole (322).

7. The reaction cell arrangement of claim 5, further comprising an optical fiber bundle (6), the optical fiber bundle (6) being used for detecting changes in the illumination intensity of the liquid in the carrying container located in the reaction Chi Lizhu (321).

8. The reaction cell arrangement of claim 7, wherein the reaction Chi Lizhu (321) has at least two fiber holes (3211) through its inner and outer walls;

at least one of the fiber holes (3211) is for mounting the fiber bundle (6) for incident light, and at least one of the fiber holes (3211) is for mounting the fiber bundle (6) for receiving light.

9. The reaction cell arrangement according to claim 8, further comprising a stationary cover (4) for fixedly connecting the reaction cell assembly (3) with the heat sink (1);

the fixed cover (4) is provided with a hollowed-out avoiding structure for the reaction Chi Lizhu (321) to penetrate out and a solid frame structure for positioning the edge of the reaction tank main body and the edge of the heating and refrigerating piece (31);

one surface of the solid frame structure, which faces the radiator (1), is abutted to the upper surface of the reaction tank main body, and the solid frame structure is fixedly connected with the radiator (1).

10. The reaction cell arrangement according to claim 9, further comprising a thermal insulation medium (7) surrounding the periphery of the reaction Chi Lizhu (321), the thermal insulation medium (7) being in contact with the stationary cover (4) on a side facing the heat sink (1).

11. The reaction cell arrangement according to claim 10, further comprising a digital board (8), said digital board (8) being located on a side of said thermal insulation medium (7) facing away from said heat sink (1);

the digital board (8) is provided with a plurality of through holes which are in one-to-one correspondence with the reactions Chi Lizhu (321) and can be exposed by the openings of the reactions Chi Lizhu (321);

the upper surface of the digital board (8) is provided with digital marks corresponding to the through holes one by one, and the upper surface of the digital board (8) is the surface of the digital board (8) opposite to the radiator (1).

12. Reaction cell arrangement according to claim 11, further comprising a connection piece (9), one end of the connection piece (9) being connected to the digital board (8), the other end of the connection piece (9) being connected to the heat sink (1).

13. A PCR instrument comprising a reaction cell arrangement according to any one of claims 1 to 12.