CN113324825A - Multi-sample tissue homogenizer - Google Patents
Multi-sample tissue homogenizer Download PDFInfo
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- CN113324825A CN113324825A CN202110618400.9A CN202110618400A CN113324825A CN 113324825 A CN113324825 A CN 113324825A CN 202110618400 A CN202110618400 A CN 202110618400A CN 113324825 A CN113324825 A CN 113324825A
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- 239000002184 metal Substances 0.000 claims abstract description 62
- 230000017525 heat dissipation Effects 0.000 claims abstract description 54
- 238000005057 refrigeration Methods 0.000 claims abstract description 47
- 238000004321 preservation Methods 0.000 claims abstract description 10
- 239000004065 semiconductor Substances 0.000 claims description 21
- 238000009529 body temperature measurement Methods 0.000 claims description 11
- 238000000265 homogenisation Methods 0.000 claims description 11
- 238000004861 thermometry Methods 0.000 claims description 7
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- 230000000712 assembly Effects 0.000 abstract description 17
- 239000007788 liquid Substances 0.000 description 22
- 238000001816 cooling Methods 0.000 description 15
- 230000000694 effects Effects 0.000 description 14
- 238000007670 refining Methods 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 7
- 230000015556 catabolic process Effects 0.000 description 6
- 238000006731 degradation reaction Methods 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 230000033228 biological regulation Effects 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
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- 238000010438 heat treatment Methods 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000005679 Peltier effect Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
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- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
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- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
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- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2866—Grinding or homogeneising
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Abstract
The invention discloses a multi-sample tissue homogenizer which comprises a base, at least two metal bath assemblies, a homogenizing assembly and a heat dissipation module, wherein the metal bath assemblies are arranged on the base and comprise heat conduction blocks, heat preservation jackets and refrigeration modules, the heat preservation jackets are arranged on the outer sides of the heat conduction blocks, the refrigeration modules are arranged between the heat preservation jackets and the heat conduction blocks, cavities are formed in the heat conduction blocks and used for placing samples, the homogenizing assemblies are arranged in the cavities and can move in the cavities, and the heat dissipation modules are connected with the refrigeration modules and used for reducing the temperature of the refrigeration modules. The invention has the advantages of saving experimental time, being controllable in temperature, simple, convenient, fast and efficient in homogenating tissues of multiple groups and multiple samples.
Description
Technical Field
The invention relates to the field of homogenizers, in particular to a multi-sample tissue homogenizer.
Background
In biological experiments, the content of protein or nucleic acid in a plurality of tissue samples needs to be tested simultaneously, so as to research the influence of the experiment on substances to be detected. In scientific experiments, various errors exist, and in order to reduce the influence of the errors on the experimental conclusion as much as possible, each treatment needs to perform measurement on a plurality of samples. During the detection process, several samples need to be homogenized. At present, a homogenizer in a laboratory can only homogenize one sample, when a certain experimental sample is homogenized, other samples need to be placed on ice to keep the sample at a low temperature, and the degradation speed of the sample is reduced by hopefully passing through the low temperature, so that the states of the samples are kept consistent, and the error is reduced. However, the time period required by the homogenizer in the laboratory to process a plurality of samples is long, and the samples are easily affected by the temperature of the laboratory, the weather condition and the environmental factors, so that the difference among the samples in the group is increased, and the experimental error is overlarge.
Disclosure of Invention
It is an object of the present invention to provide a multiple sample tissue homogenizer that addresses at least one of the problems of the prior art described above.
In order to solve the above problems, according to one aspect of the present invention, there is provided a multi-sample tissue homogenizer comprising a base, at least two metal bath assemblies mounted on the base and including a heat conduction block, a heat-insulating jacket disposed outside the heat conduction block, and a cooling module disposed between the heat-insulating jacket and the heat conduction block, the heat conduction block being provided with a cavity to house a sample, at least a portion of the homogenizing assemblies being disposed within the cavity and movable within the cavity, and the cooling module being connected to the cooling module and serving to reduce a temperature of the cooling module.
In one embodiment, the refrigeration module includes a semiconductor heat sink having opposing first and second surfaces, the first surface in direct or indirect contact with the heat conduction block, the second surface mated with the heat dissipation module.
In one embodiment, the multi-sample tissue homogenizer further comprises a thermometry module cooperating with the thermally conductive block to measure the temperature of the thermally conductive block.
In one embodiment, the multi-sample tissue homogenizer further comprises a control module, which is connected to and controls the operation of the refrigeration module, the homogenization assembly, the heat dissipation module, and the temperature measurement module.
In one embodiment, the heat dissipation module includes a heat conduction member disposed on the second surface of the semiconductor heat sink, a liquid guide tube, and a heat dissipation cold bar in fluid communication with the heat conduction member through the liquid guide tube to cool the heat conduction member.
In one embodiment, the heat dissipation module further includes a fan installed at one side of the heat dissipation cold row to dissipate heat of the heat dissipation cold row.
In one embodiment, the base comprises an upper base in which the homogenizing assembly is disposed and a lower base in which the metal bath assembly is disposed.
In one embodiment, the base further comprises a post, and the upper base and the lower base are connected by the post.
In one embodiment, the homogenizing assembly comprises a motor and a homogenizing element, the homogenizing element extending into the chamber of the heat conducting block, the motor being connected to the homogenizing element and driving the homogenizing element in motion in the chamber.
In one embodiment, the metal bath modules are independent of each other, and/or the homogenizing modules are independent of each other.
The multi-sample tissue homogenizer of the present application has at least one of the following beneficial technical effects:
firstly, the temperature is controllable. The refrigeration module and the temperature measurement module are controlled by the control module to form a temperature regulation loop, so that the temperature of the metal bath is accurately controlled, and the temperature of each sample tube and different batches of experiments is kept consistent.
Secondly, the method is convenient, fast and efficient. Through control module, realize the timing of independent homogenate subassembly and open and stop, speed control for the treatment effect, convenient operation, practicality are good.
Thirdly, the experimental time is saved. The device can simultaneously carry out tissue homogenate of multiple groups and multiple samples in a low-temperature environment, and the experimental time is saved.
Drawings
Fig. 1 is a schematic perspective view of an embodiment of the present invention.
Detailed Description
The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings so that the objects, features and advantages of the invention can be more clearly understood. It should be understood that the embodiments shown in the drawings are not intended to limit the scope of the present invention, but are merely intended to illustrate the spirit of the technical solution of the present invention.
In the following description, for the purposes of illustrating various disclosed embodiments, certain specific details are set forth in order to provide a thorough understanding of the various disclosed embodiments. One skilled in the relevant art will recognize, however, that the embodiments may be practiced without one or more of the specific details. In other instances, well-known devices, structures and techniques associated with this application may not be shown or described in detail to avoid unnecessarily obscuring the description of the embodiments.
Reference throughout this specification to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment. Thus, the appearances of the phrases "in one embodiment" or "in an embodiment" in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.
In the following description, for the purposes of clearly illustrating the structure and operation of the present invention, directional terms will be used, but terms such as "front", "rear", "left", "right", "outer", "inner", "outer", "inward", "upper", "lower", etc. should be construed as words of convenience and should not be construed as limiting terms.
The present invention relates generally to a temperature-controlled, simple, convenient, rapid, efficient device for simultaneously homogenizing multiple groups of multiple samples.
The multi-sample tissue homogenizer comprises a base, at least two metal bath assemblies, a homogenizing assembly and a heat dissipation module, wherein the metal bath assemblies are installed on the base and comprise heat conduction blocks, heat preservation jackets and refrigeration modules, the heat preservation jackets are arranged on the outer sides of the heat conduction blocks, the refrigeration modules are arranged between the heat preservation jackets and the heat conduction blocks, the heat conduction blocks are provided with cavities for placing samples, at least one part of the homogenizing assembly is arranged in the cavities and can move in the cavities, and the heat dissipation module is connected with the refrigeration modules and used for reducing the temperature of the refrigeration modules.
When a sample needs to be homogenized, the homogenizing assembly is taken out of a cavity of a heat conducting block of the metal bath assembly, so that a disposable sample tube can be placed in the cavity of the heat conducting block of the metal bath assembly, the disposable sample tube is placed in the cavity of the heat conducting block of the metal bath assembly, then the multi-sample tissue homogenizer is connected with an external power supply, a refrigeration module starts to work, the temperature is reduced, the low temperature is transmitted to the disposable sample tube through the heat conducting block, so that the sample is in a low-temperature state, meanwhile, a heat insulation jacket arranged on the outer side of the heat conducting block has the effect of effectively reducing heat exchange between the sample tube and the room temperature, the refrigeration efficiency of the refrigeration module is improved, meanwhile, a heat dissipation module connected with the refrigeration module also works to reduce the temperature of the refrigeration module, the refrigeration effect of the refrigeration module is more obvious, the metal bath assembly and the heat dissipation module are mutually matched, so that the sample keeps low temperature, the degradation speed of the sample is reduced, so that the state of the sample is kept consistent, and the error is reduced. In addition, the homogenizing assembly is arranged in the cavity of the heat conducting block and moves to homogenize the sample. And the metal bath assembly, the homogenizing assembly and the heat dissipation module are all fixedly arranged on the base.
In an embodiment of the present invention, the cooling module may also be a semiconductor heat sink. When a sample needs to be homogenized, the homogenizing assembly is taken out of a cavity of a heat conducting block in the metal bath assembly, so that a disposable sample tube can be placed in the cavity of the heat conducting block in the metal bath assembly, the disposable sample tube is placed in the cavity of the heat conducting block in the metal bath assembly, then the multi-sample tissue homogenizer is connected with an external power supply, the semiconductor radiating fin starts to work, the refrigerating end of the semiconductor radiating fin reduces the temperature and conducts low temperature to the disposable sample tube through the heat conducting block through low-temperature contact, so that the sample is in a low-temperature state, meanwhile, the heat-insulating jacket arranged on the outer side of the heat conducting block has the function of effectively reducing the heat exchange between the sample tube and the room temperature, the refrigerating efficiency of the semiconductor radiating fin is improved, and meanwhile, the radiating module connected with the heating end of the semiconductor radiating fin also works to reduce the temperature of the heating end of the semiconductor radiating fin, so that the refrigerating effect of the cooling end of the semiconductor radiating fin is more obvious, the metal bath component and the heat dissipation module are matched with each other, so that the sample is kept at low temperature, the degradation speed of the sample is reduced, the state of the sample is kept consistent, and errors are reduced. In addition, the homogenizing assembly is arranged in the cavity of the heat conducting block and moves to homogenize the sample.
In an embodiment of the present invention, the multi-sample tissue homogenizer further comprises a temperature measurement module, which is in contact with the heat-conducting block and is used for measuring the temperature of the heat-conducting block. When the homogenate is handled to the sample to needs, take out in the cavity of heat conduction piece in the subassembly is bathed to the homogenate subassembly follow metal, make can place disposable sample cell in the cavity that heat conduction piece was equipped with in the subassembly is bathed to the metal, disposable sample cell is placed and is connected external power with many sample tissue homogenizer behind the cavity of heat conduction piece in the subassembly is bathed to the metal, refrigeration module begins work, reduce the temperature and pass through the heat conduction piece conduction with the low temperature and give disposable sample cell and make the sample be in the low temperature state, the effect of placing the heat preservation overcoat in the heat conduction piece outside simultaneously is effectively to reduce the heat exchange of sample cell and room temperature, improve refrigeration module's refrigeration efficiency, the radiating module that is connected with refrigeration module simultaneously also is being used for reducing refrigeration module's temperature at work, make refrigeration module's refrigeration effect more showing. And the temperature measuring module contacted with the heat conducting block measures the temperature of the heat conducting block in the working process of the multi-sample tissue homogenizer. The metal bath component and the heat dissipation module are matched with each other, so that the sample is kept at low temperature, the degradation speed of the sample is reduced, the state of the sample is kept consistent, and errors are reduced. In addition, the homogenizing assembly is arranged in the cavity of the heat conducting block and moves to homogenize the sample.
In an embodiment of the present invention, the multi-sample tissue homogenizer further includes a control module, the control module is in signal communication with the refrigeration module, the homogenization assembly, the heat dissipation module and the temperature measurement module, and the control module controls the operation of the refrigeration module, the homogenization assembly, the heat dissipation module and the temperature measurement module. When a sample needs to be homogenized, the homogenizing assembly is taken out of a cavity of a heat conducting block in the metal bath assembly, so that a disposable sample tube can be placed in the cavity formed by the heat conducting block in the metal bath assembly, the disposable sample tube is placed in the cavity of the heat conducting block in the metal bath assembly, then the multi-sample tissue homogenizer is connected with an external power supply, a temperature measuring module in contact with the heat conducting block starts to work, when the temperature measuring module measures that the temperature of the heat conducting block is higher than the required temperature, the temperature measuring module transmits information to a refrigerating module through a control module to control the refrigerating module to start refrigerating, and meanwhile, the control module transmits a signal to a heat dissipation module to control the heat dissipation module to cooperate with the refrigerating module, so that the refrigerating efficiency of the refrigerating module is higher; when the temperature measuring module measures that the temperature of the heat conducting block is lower than the required temperature, the temperature measuring module transmits information to the refrigerating module through the control module to control the refrigerating module to stop refrigerating, meanwhile, the control module transmits signals to the heat dissipation module to control the heat dissipation module to stop heat dissipation, and temperature measurement and control are achieved and the temperature of a sample is stabilized through the control module. In addition, the control module controls the movement of the homogenizing assembly in the cavity of the heat-conducting block to homogenize the sample. The temperature control device comprises at least two metal bath components, the refrigeration module and the temperature measurement module are controlled by a circuit to form a temperature regulation loop, the temperature of each metal bath is regulated and stabilized, and the cavity is reserved on the metal block according to the size and the specification of the disposable sample tube, so that the temperature of each sample tube and different batches of experiments can be kept consistent. In addition, the motor is controlled by the circuit, so that the homogenate rod can be started and stopped at fixed time, and the speed is adjusted, thereby accelerating the processing effect and facilitating the operation.
In an embodiment of the invention, the heat dissipation module of the multi-sample tissue homogenizer comprises a heat conduction member, a liquid guide tube and a heat dissipation cold bar, when a sample needs to be homogenized, the homogenizing assembly is taken out from a cavity of a heat conduction block in a metal bath assembly, so that a disposable sample tube can be placed in a cavity arranged in the heat conduction block in the metal bath assembly, the multi-sample tissue homogenizer is connected with an external power supply after the disposable sample tube is placed in the cavity of the heat conduction block in the metal bath assembly, the refrigeration module starts to work, the temperature is reduced, the low temperature is conducted to the disposable sample tube through the heat conduction block, so that the sample is in a low-temperature state, meanwhile, a heat insulation outer sleeve arranged on the outer side of the heat conduction block is used for effectively reducing the heat exchange between the sample tube and the room temperature, the refrigeration efficiency of the refrigeration module is improved, and meanwhile, the heat dissipation cold bar cools the liquid transmitted by the heat conduction member through the liquid guide tube, make refrigeration module's refrigeration effect more showing, metal bath subassembly, heat dissipation module mutually support for the sample keeps the low temperature, reduces the degradation speed of sample, thereby makes the state of sample keep unanimous, reduces the error.
In an embodiment of the present invention, the heat dissipation module of the multi-sample tissue homogenizer further comprises a fan, when a sample needs to be homogenized, the homogenizing assembly is taken out from the cavity of the heat conduction block in the metal bath assembly, so that the disposable sample tube can be placed in the cavity of the heat conduction block in the metal bath assembly, the multi-sample tissue homogenizer is connected to an external power supply after the disposable sample tube is placed in the cavity of the heat conduction block in the metal bath assembly, the refrigeration module starts to operate, the temperature is reduced, the low temperature is conducted to the disposable sample tube through the heat conduction block, so that the sample is in a low temperature state, meanwhile, the heat insulation jacket placed outside the heat conduction block has the function of effectively reducing the heat exchange between the sample tube and the room temperature, the refrigeration efficiency of the refrigeration module is improved, meanwhile, the heat dissipation cold drain cools the liquid transmitted by the heat conduction member through the liquid guide tube, and the fan further accelerates the cooling of the liquid transmitted by the heat conduction member through the liquid guide tube, make refrigeration module's refrigeration effect more showing, metal bath subassembly, heat dissipation module mutually support for the sample keeps the low temperature, reduces the degradation speed of sample, thereby makes the state of sample keep unanimous, reduces the error. In addition, the homogenizing assembly is arranged in the cavity of the heat conducting block and moves to homogenize the sample. And the metal bath assembly, the homogenizing assembly and the heat dissipation module are all fixedly arranged on the base.
In an embodiment of the present invention, the base of the multi-sample tissue homogenizer includes an upper base and a lower base, the upper base and the lower base can be spliced into an integral fixed installation metal bath assembly, homogenizing assembly and heat dissipation module, and can be separated from each other to separate the metal bath assembly from the homogenizing assembly and leave a space for placing a disposable sample tube. Optionally, the upper base and the lower base are provided with cavities, and the metal bath assembly, the homogenizing assembly and the heat dissipation module can be fixed in the cavities; or the lower base is provided with a cavity, so that the metal bath component can be fixedly arranged in the cavity of the lower base, the upper base is only a flat plate vertical to the side wall of the lower base, the flat plate can be inserted into the side wall of the lower base and fixed with the side wall of the lower base, and the homogenizing component is fixedly arranged on the flat plate; in addition, other forms of upper base and lower base which can realize the fixed installation of the metal bath component, the homogenizing component and the heat dissipation module can be adopted.
In one embodiment of the present invention, the homogenizing element of the multi-sample tissue homogenizer is fixedly mounted to the upper base. The homogenizing assembly is fixedly arranged on the upper base and is positioned right above the level of the metal bath assembly, so that the homogenizing assembly can move in a cavity of a heat conducting block of the metal bath assembly to homogenize a sample.
In one embodiment of the present invention, the metal bath components of the multi-sample tissue homogenizer are independent of each other, and the homogenizing components are independent of each other, so that, during the operation of the multi-sample tissue homogenizer, homogenization of each sample can be performed independently, for example, homogenization can be performed on a plurality of sample tubes at the same time; or only one sample tube can be placed to homogenize one sample; it is also possible to homogenize a portion of the sample while the other portion is refrigerated. In addition to this, it is also possible to subject only the sample to a homogenization treatment or only the sample to a refrigeration treatment. Homogenizing and refrigerating different samples according to different requirements, so that energy is saved.
One embodiment of a multiple sample tissue homogenizer according to the present invention is described below with reference to fig. 1.
Fig. 1 is a schematic perspective view of a multiple sample tissue homogenizer 100 according to an embodiment of the present invention. As shown in FIG. 1, a multi-sample tissue homogenizer 100 comprises a base 10, six metal bath assemblies 20, six homogenizing assemblies 30, a heat sink module 40, a thermometry module 50, and a control module 60. The base 10 is composed of an upper base 11, an upright post 12 and a lower base 13 from top to bottom in the vertical direction, the upper base 11 and the lower base 13 are both quadrilateral frame plate structures, the upper base 11 and the lower base 13 are spaced at a certain distance in the vertical direction, and the upper base 11 and the lower base 13 are both opposite and have a cavity so that an accommodating space is formed below the upper base 11 in the vertical direction and above the lower base 13 in the vertical direction. The upright 12 is vertically fixed to the lower base 13 and connects and supports the upper base 11, and the upper base 11, the upright 12 and the lower base 13 are fixed to each other and form an integral stand which can be moved freely. Alternatively, the upper base 11 can be removed from the fixed unit of the lower base 13 and the column 12, and a space is left for the chamber 24 of the heat-conducting block 21 in the metal bath assembly 20 to place a sample tube, and the upper base 11 can still be mounted on the column 12 after placing the sample tube. The upper base 11, the upright 12 and the lower base 13 are fixed to each other and form an integral stand.
Although in the embodiment shown in fig. 1, multi-sample tissue homogenizer 100 comprises six metal bath assemblies 20, six homogenizing assemblies 30, one thermal module 40, one thermometry module 50, and one control module 60, in other embodiments, multi-sample tissue homogenizer 100 may comprise greater than or less than six metal bath assemblies 20, such as two or three metal bath assemblies 20, and may also comprise greater than or less than six homogenizing assemblies 30, such as two or three homogenizing assemblies 30. In addition, in other embodiments, a plurality of heat dissipation modules 40, temperature measurement modules 50, control modules 60, and the like may be included.
The metal bath assembly 20 includes a heat conducting block 21, a semiconductor heat sink 22, and a heat insulating jacket 23. The heat conducting block 21 is provided with a chamber 24 for placing a sample tube (not shown); the heat-insulating jacket 23 is disposed outside the heat-conducting block 21 to reduce heat exchange between the sample tube placed in the chamber 24 and room temperature. The semiconductor heat sink 22 is disposed between the heat insulating jacket 23 and the heat conductive block 21. The semiconductor cooling fin is also called a semiconductor refrigerating fin and is also called a thermoelectric refrigerating fin, and the aim of refrigerating can be fulfilled by utilizing the Peltier effect of semiconductor materials and respectively absorbing heat and releasing heat at two ends of a couple when direct current passes through the couple formed by connecting two different semiconductor materials in series. The refrigeration end of the semiconductor cooling fin 22 is matched with the heat conducting block 21 and transmits the low temperature to the sample tube arranged in the cavity 24 through the heat conducting block 21, and the hot end of the semiconductor cooling fin 22 is matched with the cooling module 40 and takes away the heat through the cooling module 40. Alternatively, the six metal bath assemblies 20 of FIG. 1 are independent of each other and fixedly mounted on the lower base 13.
In one embodiment, heat dissipation module 40 includes a thermally conductive member 41, a conduit 42, a heat dissipating cold row 43, and a fan 44. The heat conducting member 41 is in contact connection with the hot end of the semiconductor heat sink 22, the heat dissipation cold row 43 is in fluid communication with the water-cooling heat conducting block 41 through a liquid guide tube 42, the heat dissipation cold row 43 and the fan 44 are both nested and mounted on the side wall of the lower base 13, the heat dissipation cold row 43 is mounted on the inner side of the cavity of the lower base 13, and the fan 44 is mounted on the outer side of the cavity of the lower base 13. The heat dissipation cold row 43 cools the liquid transferred from the heat conduction member 41 through the liquid guide tube 42, and the fan 44 further accelerates the cooling of the liquid transferred from the heat conduction member 41 through the liquid guide tube 42. Alternatively, six heat-conducting members 41 are mounted on each of the six metal bath assemblies 20, and each of the six heat-conducting members 41 is connected to a heat-dissipating cold row 43 and a fan 44 via liquid guide tubes 42. Alternatively, the heat conducting member 41 is a water-cooled heat conducting block, and the liquid flowing through the liquid guiding tube 42 is water. The heat-conducting member 41 may be a metal heat-conducting block or the like.
It should be noted that the heat dissipation module 40 including the heat conduction member 41, the liquid guide tube 42, the heat dissipation cold air duct 43 and the fan 44 is only an alternative implementation manner of the heat dissipation module 40, and in other embodiments, the heat dissipation module 40 may be implemented in other manners, for example, the fan 44 may not be included.
Continuing to refer to fig. 1 for a description of the refining assembly 30 according to an embodiment of the present invention, the refining assembly 30 includes a refining element 32, the refining element 32 extends into the chamber 24 of the heat conducting block 21 to homogenize the sample in the chamber 24, and optionally, the refining assembly 30 may further include a control motor 31 to manually or otherwise control the movement of the refining element 32, and the refining element 32 is connected to the control motor 31 and controls the operation of the refining assembly 30 through the control motor 31. In the embodiment shown in fig. 1, the control motor 31 and the homogenizing assembly 30 are fixedly mounted on the upper base 11, the rotating shaft of the control motor 31 is connected with the homogenizing element 32, and the homogenizing element 32 can be rod-shaped, spiral-shaped, rotary-knife-shaped, and the like to realize the shape of homogenizing.
In one embodiment of the present invention, the multi-sample tissue homogenizer 100 may further comprise a thermometry module 50 and a control module 60, the thermometry module 50 in signal communication with the control module 60 and in contact with the thermally conductive block 21 to measure the temperature of the thermally conductive block 21 and transmit the temperature to the control module 60 for temperature control, for example, by forming a temperature regulation loop with the thermometry module 50, the control module 60 regulates and stabilizes each metal bath temperature. The control module 60 may be mounted on the upper base 11, for example, and optionally, the control module 60 is in signal communication with the homogenizing assembly 30, the heat sink module 40, and the temperature module 50 to control the operation of the homogenizing assembly 30, the heat sink module 40, and the temperature module 50. The temperature measuring module 50 may be implemented by a resistance thermometer, a pressure thermometer, or the like.
When the sample needs to be homogenized, the sample tube is placed in the cavity 24 of the heat conduction block 21, the power supply is switched on, and the homogenizing assembly 30, the heat dissipation module 40 and the temperature measurement module 50 start to work and stir the sample for homogenization. The temperature measuring module 50 transmits the temperature information of the heat conducting block 21 to the control module 60, the control module 60 compares the temperature with a preset temperature, if the temperature is higher than the preset temperature, the control module 60 controls the heat dissipation module 40 to work, the heat dissipation cold bar 43 cools the heat conducting member 41 in contact with the semiconductor heat sink 22 through the liquid transmitted by the liquid guide tube 42, and the fan 44 further accelerates the cooling of the liquid from the heat conducting member 41 through the liquid guide tube 42, so as to strengthen the heat dissipation effect of the heat dissipation cold bar 43. The control module 60 can also adjust the speed of the homogenizing motor 31 and control the timing start and stop of the homogenizing motor through the received information.
As shown in fig. 1, since the six metal bath modules 20 and the six homogenization modules 30 of the multi-sample tissue homogenizer 100 are independently connected, the multi-sample tissue homogenizer 100 shown in fig. 1 can simultaneously perform timed start-stop and speed adjustment of the single or multiple metal bath modules 20 and homogenization modules 30.
In conclusion, the multi-sample tissue homogenizer has the beneficial technical effects of controllable temperature, simplicity, convenience, high efficiency, rapidness, experiment time saving, capability of homogenizing tissues of multiple groups simultaneously and the like.
According to the invention, each independent low-temperature metal bath is independently controlled by the circuit, a plurality of samples are homogenized simultaneously, the problem that the homogenizer in a laboratory can only homogenize one sample at present is solved, and the problems of long time period and the like of the sample homogenization at present are solved by controlling the independent homogenizing component by the control module. Compared with domestic similar research and product achievements, the low-temperature multi-sample tissue homogenizer at least has the following beneficial technical effects:
firstly, the temperature is controllable. The refrigeration module and the temperature measurement module are controlled by the control module to form a temperature regulation loop, so that the temperature of the independent low-temperature metal bath is accurately controlled, and the temperature of each sample tube and different batches of experiments is kept consistent.
Secondly, the method is convenient, fast and efficient. The motor is controlled through the control module, the timing start and stop of the independent homogenate component are realized, the speed is adjusted, the processing effect is accelerated, the operation is convenient, and the practicability is good.
Thirdly, the experimental time is saved. The device can simultaneously carry out tissue homogenate of multiple groups and multiple samples in a low-temperature environment, and the experimental time is saved.
While the preferred embodiments of the present invention have been illustrated and described in detail, it should be understood that various changes and modifications of the invention can be effected therein by those skilled in the art after reading the above teachings of the invention. Such equivalents are intended to fall within the scope of the claims appended hereto.
Claims (10)
1. The utility model provides a many samples tissue homogenizer, its characterized in that, many samples tissue homogenizer includes base, two at least metal bath subassemblies, homogenate subassembly and thermal module, metal bath subassembly install in on the base and including heat conduction piece, heat preservation overcoat and refrigeration module, the heat preservation overcoat set up in the heat conduction piece outside, refrigeration module set up in heat preservation overcoat with between the heat conduction piece, the heat conduction piece is equipped with the cavity in order to place the sample, at least some of homogenate subassembly set up in the cavity and can be in the cavity internal motion, and thermal module with refrigeration module is connected and is used for reducing refrigeration module's temperature.
2. The multi-sample tissue homogenizer of claim 1, wherein the refrigeration module comprises a semiconductor heat sink having opposing first and second surfaces, the first surface in direct or indirect contact with the heat conduction block, the second surface cooperating with the heat dissipation module.
3. The multi-sample tissue homogenizer of claim 1, further comprising a thermometry module cooperating with the thermally conductive block to measure the temperature of the thermally conductive block.
4. The multi-sample tissue homogenizer of claim 3, further comprising a control module connected to and controlling the operation of the refrigeration module, homogenization assembly, heat dissipation module, and temperature measurement module.
5. The multi-sample tissue homogenizer of claim 2, wherein the heat dissipation module comprises a thermally conductive member disposed on the second surface of the semiconductor heat sink, a fluid conduit, and a heat sink cold bar in fluid communication with the thermally conductive member via the fluid conduit to cool the thermally conductive member.
6. The multi-sample tissue homogenizer of claim 5, wherein the heat dissipation module further comprises a fan mounted on one side of the heat dissipation cold row to dissipate heat therefrom.
7. The multi-sample tissue homogenizer of claim 1, wherein the base comprises an upper base and a lower base, the homogenizing assembly being disposed within the upper base, and the metal bath assembly being disposed within the lower base.
8. The multi-sample tissue homogenizer of claim 7, wherein the base further comprises a post, and the upper base and the lower base are connected by the post.
9. The multi-sample tissue homogenizer of claim 1, wherein the homogenizing assembly comprises a motor and a homogenizing element, the homogenizing element extending into the chamber of the heat-conducting block, the motor being coupled to the homogenizing element and driving the homogenizing element in motion within the chamber.
10. The multi-sample tissue homogenizer of claim 1, wherein the metal bath components are independent of each other, and/or the homogenizing components are independent of each other.
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| CN202110618400.9A CN113324825A (en) | 2021-06-03 | 2021-06-03 | Multi-sample tissue homogenizer |
| GB2207642.6A GB2608698B (en) | 2021-06-03 | 2022-05-25 | Multi-sample tissue homogenizer |
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| GB2608698B (en) * | 2021-06-03 | 2023-09-13 | Univ Zhejiang | Multi-sample tissue homogenizer |
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| GB2608698A (en) | 2023-01-11 |
| GB202207642D0 (en) | 2022-07-06 |
| GB2608698B (en) | 2023-09-13 |
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