CN220767019U - Nucleic acid amplification device - Google Patents
Nucleic acid amplification device Download PDFInfo
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- CN220767019U CN220767019U CN202322267640.6U CN202322267640U CN220767019U CN 220767019 U CN220767019 U CN 220767019U CN 202322267640 U CN202322267640 U CN 202322267640U CN 220767019 U CN220767019 U CN 220767019U
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- 230000003321 amplification Effects 0.000 title claims abstract description 119
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 119
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 25
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 25
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 25
- 230000007246 mechanism Effects 0.000 claims abstract description 58
- 230000001105 regulatory effect Effects 0.000 claims abstract description 24
- 238000009413 insulation Methods 0.000 claims description 64
- 238000010438 heat treatment Methods 0.000 claims description 32
- 238000004321 preservation Methods 0.000 claims description 30
- 239000004020 conductor Substances 0.000 claims description 26
- 238000007667 floating Methods 0.000 claims description 20
- 239000004065 semiconductor Substances 0.000 claims description 20
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 19
- 239000000741 silica gel Substances 0.000 claims description 19
- 229910002027 silica gel Inorganic materials 0.000 claims description 19
- 238000001816 cooling Methods 0.000 claims description 11
- 230000006835 compression Effects 0.000 claims description 9
- 238000007906 compression Methods 0.000 claims description 9
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 36
- 238000006243 chemical reaction Methods 0.000 abstract description 15
- 238000001704 evaporation Methods 0.000 abstract description 7
- 230000008020 evaporation Effects 0.000 abstract description 7
- 230000009286 beneficial effect Effects 0.000 abstract description 6
- 230000002401 inhibitory effect Effects 0.000 abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 3
- 238000005842 biochemical reaction Methods 0.000 abstract description 2
- 239000002904 solvent Substances 0.000 abstract 1
- 230000017525 heat dissipation Effects 0.000 description 33
- 239000002699 waste material Substances 0.000 description 5
- 230000009471 action Effects 0.000 description 4
- 238000000137 annealing Methods 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000012257 pre-denaturation Methods 0.000 description 3
- 230000033228 biological regulation Effects 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 238000004134 energy conservation Methods 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 239000002470 thermal conductor Substances 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000010230 functional analysis Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
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- 230000005855 radiation Effects 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000012916 structural analysis Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000005496 tempering Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a nucleic acid amplification device, which belongs to a biochemical reaction device and comprises a bottom temperature regulating mechanism, a heat cover and an opening and closing mechanism, wherein the bottom temperature regulating mechanism is used for bearing and accommodating a plurality of amplification containers and regulating the temperature of the bottoms of the amplification containers. The thermal cover is used to regulate the temperature of the top cover at the top of the amplification vessel from above, and the bottom is a flexible structure. The opening and closing mechanism is connected with the bottom temperature adjusting mechanism and the heat cover and is used for driving the heat cover to move. The utility model has the beneficial effects that: when the bottom temperature adjusting mechanism heats the bottom of the amplification container, the heat cover is simultaneously abutted against the top covers of all the amplification containers to heat the top covers, so that the temperature of the pipe wall of the upper half part of the amplification container is higher than the temperature of the reagent, thereby inhibiting the evaporation of water and the volatilization of solvents of a reaction system in the amplification container, and ensuring that the top covers of all the amplification containers are uniformly heated.
Description
Technical Field
The utility model relates to a nucleic acid amplification device, which belongs to a biochemical reaction device.
Background
In the field of biochemistry, in particular in the technical field of enzymology or microbiology devices, heating of the reagents is often a very important link. Taking PCR as an example, PCR, namely polymerase chain reaction, is an experimental technique for amplifying a segment of DNA to a sufficient quantity under the joint participation of DNA polymerase and nucleotide substrates by taking the segment of DNA as a template so as to carry out structural and functional analysis, and a PCR detection method has great significance in the aspects of rapidly diagnosing bacterial infectious diseases clinically and the like. In the reaction process of PCR, the steps of pre-denaturation, annealing, extension, heat preservation and preservation are needed, the reaction system is required to be heated for a plurality of times, in the heating process, the temperature of the reagent is higher than the ambient temperature, the moisture in the reagent is evaporated along with the temperature rise, and the reagent is condensed and reflowed on a pipe cover, so that the concentration of the reagent is changed continuously, and the concentration change is unfavorable for the reaction due to the fact that the concentration of the reagent is adapted to the reaction requirement. In order to reduce the concentration variation caused by such evaporation, a heat cover is usually added to the top of the reagent tube, and the heat cover is abutted against the top of the reagent tube and heated so that the temperature above the reagent in the reagent tube is higher than the temperature of the reagent, thereby suppressing the evaporation. However, in order to sufficiently heat the reagent tube, the heat cover is required to be in sufficient contact with the tube cover of the reagent tube, and the heights of the plurality of reagent tubes are not uniform, so that it is difficult to satisfy the requirement that the amplification of a plurality of sets of nucleic acids be performed simultaneously.
Disclosure of Invention
The purpose of the present utility model is to provide a nucleic acid amplification device capable of reducing evaporation of moisture in a reagent.
In order to achieve the above purpose, the present utility model provides the following technical solutions:
a nucleic acid amplification device comprising;
the bottom temperature adjusting mechanism is used for bearing a plurality of amplification containers and adjusting the temperature of the bottoms of the amplification containers, and the top of each amplification container is provided with a top cover;
a thermal cover for heating the top of the top cover of all the amplification containers, the bottom of the thermal cover being of a flexible structure;
the opening and closing mechanism is arranged between the bottom temperature adjusting mechanism and the heat cover, so that the heat cover has freedom degrees in the transverse direction and the longitudinal direction, and is far away from or props against the top cover of the amplified container.
Optionally, the thermal cover comprises
The silica gel heating piece is arranged at the bottom of the heat cover and is used for abutting against and heating the top cover of all the amplification containers.
And the bearing plate is arranged at the top of the heat cover and is used for connecting the opening and closing mechanism and bearing the silica gel heating sheet.
Optionally, the opening and closing mechanism includes:
the rail piece comprises a plurality of linear rails with the same direction, a first end of each linear rail is connected with the bottom temperature adjusting mechanism, and a second end of each linear rail is far away from the bottom temperature adjusting mechanism;
the sliding piece is connected to the linear track in a sliding way, the sliding piece is structurally suitable for the bottom temperature adjusting mechanism, and when reaching the first end of the linear track, the sliding piece is nested outside the bottom temperature adjusting mechanism and is contacted with the bottom temperature adjusting mechanism;
the two ends of the driving piece are respectively connected with the sliding piece and the bottom temperature adjusting mechanism, the driving piece is parallel to the track piece, and the two ends of the driving piece extend in opposite directions and are used for driving the sliding piece to slide along the track piece;
and a floating assembly connecting the slider and the thermal cover and providing the thermal cover with a longitudinal degree of freedom.
Optionally, the floating assembly comprises a floating rod vertically arranged at the top of the sliding piece, a compression spring nested outside the floating rod and a sliding through hole arranged on the heat cover and adapted to the floating rod.
Optionally, the thermal cover further comprises a thermal insulation board, and the thermal insulation board is arranged between the bearing board and the thermal cover.
Optionally, the bottom tempering mechanism comprises:
the second heat preservation bin is constructed into a box-packed structure with a hollowed bottom, a plurality of second pipe support holes are formed in the top of the second heat preservation bin, the amplification container is erected above the second pipe support holes, and the inner walls of the second pipe support holes are adapted to the outer walls of the amplification container;
the heat conductor comprises a base at the bottom and a plurality of heat conducting sleeves above the base, the base is directly or indirectly connected with the hollowed-out position at the bottom of the second thermal insulation bin to form an inner space with the closed bottom, the position of the heat conducting sleeve corresponds to the second pipe support hole, the structure is suitable for the bottom structure of the amplification container, and when the amplification container is erected above the second pipe support hole, the outer wall of the bottom of the amplification container is abutted against the inner wall of the heat conducting sleeve;
the first temperature regulating piece is positioned below the heat conductor and is contacted with part of the bottom surface of the base of the heat conductor.
Optionally, the bottom temperature adjusting mechanism further comprises a temperature measuring piece, wherein the temperature measuring piece is arranged on the inner wall of the thermal guide sleeve and is in contact with the outer wall of the bottom of the amplification container.
Optionally, the bottom temperature adjusting mechanism further comprises a first thermal insulation bin, the first thermal insulation bin is constructed into a box-packed structure with a hollowed bottom, the first thermal insulation bin is nested inside the second thermal insulation bin, the hollowed bottom of the first thermal insulation bin is embedded with the base of the heat conductor, the bottom of the first thermal insulation bin is connected with the bottom of the second thermal insulation bin to form a closed space, a plurality of first pipe rack holes are formed in the top of the first thermal insulation bin, and the first pipe rack holes are located between the heat conduction sleeve and the second pipe rack holes and are adapted to the outer wall of the amplification container.
Optionally, the first temperature regulating piece is a semiconductor refrigerating piece, a heat dissipation component is connected to the bottom of the semiconductor refrigerating piece, and a heat insulation cavity is formed between the upper surface of the heat dissipation component, the lower surface of the heat conductor base, the side surface of the semiconductor refrigerating piece and the inner wall of the first heat insulation bin.
Optionally, the heat dissipation assembly comprises a heat dissipation plate connected with the bottom of the semiconductor refrigeration plate and a heat dissipation fan connected with the bottom of the heat dissipation plate.
The utility model has the beneficial effects that: the opening and closing mechanism drives the heat cover to reach the upper part of the bottom temperature regulating mechanism, the bottom temperature regulating mechanism heats the bottom of the amplification container, namely the reagent of the reaction system, and the heat cover heats the top cover of the amplification container, so that the temperature of the pipe wall of the upper half part of the amplification container is higher than the temperature of the reagent, thereby inhibiting the evaporation of water in the reagent, and reducing the risk that the reaction is affected by the change of the concentration of the reagent. Because the bottom of the heat cover is of a flexible structure, when the heights of the top covers of the amplification containers are slightly different, the top covers of the amplification containers can be abutted against the bottom of the heat cover under the action of the elastic force of the bottom of the heat cover, so that the heat cover is suitable for simultaneous amplification of multiple groups of reaction systems.
Further, the rigid bearing plate is used as a mechanical structure for connecting the opening and closing mechanism, and the silica gel heating plate is connected to the bottom of the bearing plate, so that connection is facilitated. The silica gel heating plate has the advantages of uniform heating and higher elasticity, and is favorable for the heating uniformity of the top cover of each amplification container.
Further, when the nucleic acid amplification device heats, the bearing plate is positioned above the bottom temperature adjusting mechanism, the sliding piece is nested outside the bottom temperature adjusting mechanism and is contacted with the bottom temperature adjusting mechanism, and the silica gel heating piece is abutted to the top of the top cover of the amplification container from the upper part, so that the heat is fully transferred, the heat dissipation is reduced, and the heat preservation and the energy conservation of the upper half part of the amplification container are facilitated. After heating, the bearing plate upwards keeps away from the top of the top cover of the amplification container, and the bearing plate is prevented from sliding along with the opening and closing mechanism to the side due to friction force.
Further, the heat insulation plate is arranged on the bearing plate and the heat cover, so that the high temperature of the heat cover is prevented from affecting the mechanical strength of the bearing plate, the service life of the bearing plate is prolonged, and the risk of device faults is reduced.
Further, the heat conductor has good heat conductivity, can conduct heat with lower loss rate, and effectively keeps the temperature of different positions on the surface at the same level, thereby being beneficial to keeping the temperature uniformity of each amplification container, and the second heat preservation bin reduces heat dissipation and reduces energy waste.
Further, the temperature of the outer wall of the bottom of the amplification container is fed back in real time through the temperature measuring piece, so that the temperature of the reagent of the reaction system can be controlled and regulated.
Further, the first thermal insulation bin, the heat conductor and the outer wall of the amplification container form a small airtight space, internal heat is prevented from escaping to the outside, energy is saved, the temperature of the inner wall of the heat conducting sleeve is easier to adjust, and flexible adjustment of the temperature of the reagent is facilitated. The second heat preservation storehouse, first heat preservation storehouse outer wall and amplification container outer wall form great airtight space, and the heat of amplification container's top cap passes through the amplification container pipe wall and transmits to the amplification container upper half, and the amplification container upper half is arranged in great airtight space, and the second heat preservation storehouse helps the heat preservation of amplification container upper half, reduces the energy waste, helps keeping the amplification container upper half at higher temperature.
Further, the semiconductor refrigerating sheet has the advantages of small volume, rapid temperature regulation and wide temperature regulation range, is beneficial to flexibly regulating the temperature of the reagent in the amplification container, and because the two ends of the semiconductor refrigerating sheet are used for respectively refrigerating and heating, the semiconductor refrigerating sheet needs to be regulated by a heat dissipation component to prevent heat accumulation. Because the two ends of the semiconductor refrigerating sheet have larger temperature difference, the heat-insulating cavity is used for insulating the heat conductor and the heat-radiating component, and the heating effect of the semiconductor refrigerating sheet is beneficial to optimization.
Further, the semiconductor refrigerating sheet intermittently works, the heat dissipation requirement is larger in working, the heat dissipation requirement is smaller in suspending working, the heat dissipation force is adjusted by controlling the working state of the heat dissipation fan, and the energy consumption can be reduced while the heat dissipation requirement is met.
The foregoing description is only an overview of the present utility model, and is intended to provide a better understanding of the present utility model, as it is embodied in the following description, with reference to the preferred embodiments of the present utility model and the accompanying drawings.
Drawings
FIG. 1 is a schematic diagram showing the structure of a nucleic acid amplification apparatus according to the present utility model;
fig. 2 is a cross-sectional view at A-A in fig. 1.
Legend description: 101. an amplification vessel; 102. a top cover; 1. a bottom temperature adjusting mechanism; 11. a first thermal insulation bin; 111. a first pipe rack hole; 12. a second thermal insulation bin; 121. a second pipe rack hole; 13. a thermal conductor; 131. a base; 132. a hot guide sleeve; 14. a first temperature adjusting member; 141. a heat insulating chamber; 15. a temperature measuring member; 16. a heat dissipation assembly; 161. a heat sink; 162. a heat radiation fan; 2. an opening and closing mechanism; 21. a driving member; 211. a motor; 212. a screw rod; 22. a track member; 221. a linear rail; 23. a slider; 231. sliding side surfaces; 232. a connection surface; 24. a floating assembly; 241. a floating rod; 2411. a limit part; 242. a compression spring; 243. a sliding through hole; 3. a thermal cover; 31. a silica gel heating sheet; 32. a heat insulating plate; 33. a carrying plate; 4. and a controller.
Detailed Description
The following description of the embodiments of the present utility model will be made apparent and fully in view of the accompanying drawings, in which some, but not all embodiments of the utility model are shown. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
In the description of the present utility model, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art. In addition, the technical features of the different embodiments of the present utility model described below may be combined with each other as long as they do not collide with each other.
Referring to fig. 1, a nucleic acid amplification apparatus according to a preferred embodiment of the present utility model includes a bottom temperature adjusting mechanism 1, an opening and closing mechanism 2, and a thermal cover 3, wherein the bottom temperature adjusting mechanism 1 carries a plurality of amplification vessels 101 and adjusts the temperature of the bottom of the amplification vessels 101. The top of the amplification vessel 101 is provided with a top cover 102. The thermal cover 3 heats the top cover 102 of the amplification vessel 101 from the top to heat the tube wall of the upper half of the amplification vessel 101, and the bottom of the thermal cover 3 is of a flexible structure. The opening and closing mechanism 2 is arranged between the bottom temperature adjusting mechanism 1 and the heat cover 3, drives the heat cover 3 to be close to or far away from the upper part of the bottom temperature adjusting mechanism 1 in the horizontal direction, and the heat cover 3 slides along the opening and closing mechanism 2 in the vertical direction. In this embodiment, the amplification vessel 101 is a PCR tube.
The utility model has the beneficial effects that: the opening and closing mechanism 2 drives the heat cover 3 to reach the upper part of the bottom temperature regulating mechanism 1, the bottom temperature regulating mechanism 1 heats the bottom of the amplification container 101, namely the reagent of the reaction system, and the heat cover 3 heats the top cover 102 of the amplification container 101, so that the temperature of the pipe wall of the upper half part of the amplification container 101 is higher than the temperature of the reagent, thereby inhibiting the evaporation of water in the reagent, and reducing the risk that the reaction is affected by the change of the concentration of the reagent. Because the bottom of the heat cover 3 is of a flexible structure, when the heights of the top covers 102 of the amplification containers 101 are slightly different, the top covers 102 of the amplification containers 101 can be abutted against the bottom of the heat cover 3 under the action of the elastic force of the bottom of the heat cover 3, so that the method is suitable for simultaneous amplification of multiple groups of reaction systems.
Referring to fig. 1 and 2, the bottom temperature adjustment mechanism 1 includes a first thermal insulation bin 11, a second thermal insulation bin 12, a heat conductor 13, a temperature measuring member 15, a first temperature adjusting member 14, and a heat dissipation assembly 4. The bottom of the second thermal insulation bin 12 is opened, and the first thermal insulation bin 11 is nested below the inner part of the second thermal insulation bin 12, so that the bottom of the second thermal insulation bin 12 is closed. The bottom of the first heat preservation bin 11 is hollowed, and the heat conductor 13 is embedded into the bottom of the first heat preservation bin 11, so that the bottom of the first heat preservation bin 11 is closed. The first temperature adjusting member 14 is located at the bottom of the heat conductor 13 and is in direct contact with the bottom of the heat conductor 13, and the heat dissipating component 16 is located at the bottom of the first temperature adjusting member 14 and is in direct contact with the bottom of the first temperature adjusting member 14.
The heat conductor 13 includes a base 131 and a heat conducting sleeve 132 uniformly arranged above the base 131, the base 131 and the heat conducting sleeve 132 are customized workpieces as a whole, sixteen heat conducting sleeves 132 are arranged in the embodiment uniformly and flush in two rows, and the outer walls of eight heat conducting sleeves 132 in each row are connected with each other. The base 131 is rectangular and flat, part of the bottom surface is in contact with the first temperature adjusting member 14, and the top surface is connected with the thermal guide sleeve 132. The outer wall of the thermal guide sleeve 132 is cylindrical, and the shape of the inner wall is adapted to the shape of the outer wall of the amplification vessel 101. The thermal guide sleeve 132 is highly suitable for the amplification container 101, the top of the thermal guide sleeve 132 is positioned at the height of one third of the volume of the amplification container 101, the sample and the reagent are stored in the amplification container 101, the storage volume is not more than one third of the volume of the amplification container 101, namely, the reagents of the reaction system are all positioned inside the thermal guide sleeve 132, so that the thermal guide sleeve 132 is convenient for heat exchange. The thermal conductor 13 has better overall thermal conductivity, can effectively keep the temperature consistent everywhere on the surface, has smaller heat loss, and is favorable for uniformly transferring the heat on the upper surface of the first temperature regulating member 14 to the outer wall of each amplification container 101.
The inner wall of the thermal guide sleeve 132 is provided with a temperature measuring member 15, in this embodiment, the temperature measuring member 15 preferably uses a temperature measuring wire, and a probe of the temperature measuring wire abuts against the outer wall of the amplification container 101, so as to feed back the temperature of the reagent in real time, and help to control and regulate the temperature of the reagent, thereby meeting the temperature conditions of reagent preservation or reaction. The temperature measuring line has the advantages of small volume, low price, high sensitivity, difficult damage and wide temperature measuring range, and is more convenient to contact the outer wall of the amplification container 101 and timely feed back the actual temperature.
In this embodiment, the first temperature adjusting member 14 preferably uses a semiconductor cooling plate, and the semiconductor cooling plate has both cooling and heating functions, is easy to adjust, and is conducive to flexibly adjusting the temperature of the reagent. Meanwhile, the semiconductor refrigerating sheet has small volume and light weight, and is more suitable for the scene of adjusting the temperature of the reagent. The two ends of the semiconductor refrigerating piece are respectively positioned on the upper surface and the lower surface, and all the upper surfaces are contacted with part of the bottom surface of the base 131 of the heat conductor 13, so that the heat on the upper surface of the first temperature regulating piece 14 is fully transferred to the heat conductor 13, and the heat dissipation waste is prevented. The entire lower surface is in contact with a portion of the top surface of the heat sink assembly 4, contributing to improved heat dissipation efficiency.
The whole rectangular box-shaped structure of first heat preservation storehouse 11, first heat preservation storehouse 11 top is the horizontality, and the bottom opening and outwards extend and be the horizontal stand form. The side of the base 131 of the heat conductor 13 is connected with the inner wall above the bottom of the first heat preservation bin 11, so that the top of the first heat preservation bin 11 is positioned above the top of the heat guide sleeve 132, and a heat preservation space is formed above the heat conductor 13; the bottom of the first heat preservation bin 11 is flush with the bottom of the first temperature regulating piece 14, and a heat insulation cavity 141 is formed with the top of the first temperature regulating piece 14 and the heat dissipation component 4. The top of the first thermal insulation bin 11 is provided with first pipe rack holes 111, the first pipe rack holes 111 are respectively located right above the thermal conductive sleeves 132, the aperture is adapted to the outer wall of the amplification container 101, and sixteen first pipe rack holes 111 are arranged in the embodiment. When the amplification container 101 is placed in the thermal guide sleeve 132, the outer wall of the amplification container 101 is abutted against the inner wall of the first pipe support hole 111, at this time, the outer wall of the amplification container 101, the inner wall of the first heat preservation bin 11 and the upper surface of the heat conductor 13 form a closed space with smaller volume, so that the heat preservation of the reagent is facilitated, the energy waste is reduced, the flexible adjustment of the temperature in the closed space is facilitated, and the rapid adjustment of the reaction temperature is facilitated.
The heat insulation cavity 141 is a hollow cavity surrounding the side surface of the first temperature adjusting member 14, and air is used as a heat insulation medium to achieve a heat insulation effect. Air is easy to obtain and fill, and has good heat insulation effect. Because the first temperature adjusting piece 14 adopts the semiconductor refrigerating piece, a larger temperature difference exists between the upper surface and the lower surface of the semiconductor refrigerating piece, so that a larger temperature difference exists between the top surface of the heat component and the bottom surface of the base 131 of the heat conductor 13, the heat insulation cavity 141 is only in contact with the side surface of the first temperature adjusting piece 14, the top surface of the heat component on the inner wall of the first heat preservation bin 11 and the bottom surface of the base 131 of the heat conductor 13, heat exchange between the top surface of the heat dissipation component 4 and the bottom surface of the base 131 of the heat conductor 13 is prevented, the inner wall temperature of the heat guide sleeve 132 is prevented from being influenced by the lower surface temperature of the first temperature adjusting piece 14, and energy waste is reduced.
The second thermal insulation bin 12 is rectangular and is of a box-shaped structure with an opening at the bottom, the opening at the bottom is nested outside the upper part of the first thermal insulation bin 11, the bottom is closed by being connected with a horizontal table extending out of the first thermal insulation bin 11, and the long side horizontally extends outside. The second thermal insulation bin 12 is highly suitable for the amplification container 101, and a second pipe rack hole 121 is formed in the top. The second pipe rack holes 121 are located directly above the first pipe rack holes 111, and sixteen positions are provided in this embodiment. When the amplification container 101 is placed in the second pipe support hole 121, the outer wall of the amplification container 101 is simultaneously abutted against the inner walls of the first pipe support hole 111, the second pipe support hole 121 and the heat conducting sleeve 132, and the top cover 102 of the amplification container 101 is positioned above the second heat preservation bin 12. The second thermal insulation bin 12 is used for preserving heat of the upper half part of the amplification container 101, when the opening and closing mechanism 2 heats the top cover 102 of the amplification container 101, heat of the top cover 102 is transferred to the middle part of the amplification container 101 along with the outer wall of the amplification container 101, and the second thermal insulation bin 12 is used for preserving temperature of the middle part of the amplification container 101, so that reagent evaporation can be restrained.
The heat dissipation assembly 16 includes the heat dissipation fin 161 and the heat dissipation fan 162, and the heat dissipation requirement of the first temperature adjusting member 116 is higher when working, and the heat dissipation efficiency is improved by the heat dissipation fin 161 and the heat dissipation fan 162. When the first temperature adjusting member 116 is in a halt operation, the heat dissipation requirement is low, and at this time, the heat dissipation fan 162 can be turned off, only the heat dissipation is performed through the heat dissipation fins 161, no power is needed, and the energy consumption is reduced.
The heat cover 3 has a rectangular plate-like structure. The top of the heat cover 3 is provided with a bearing plate 33, and the bearing plate 33 has larger mechanical strength and plays a bearing and connecting role. The bottom surface of the bearing plate 33 is connected with a heat insulation plate 32, and the bottom surface of the heat insulation plate 32 is connected with a silica gel heating plate 31. Since the bearing plate 33 is slidably connected to the floating rod 241, a high requirement is placed on the shape of the bearing plate 33 to ensure smooth sliding. When the thermal cover 3 is in operation, the mechanical structure of the carrier plate 33 is easily affected. By adding the heat insulating plate 32, the risk that the bearing plate 33 is influenced by the heat cover 3 to deform or reduce strength is reduced, and the service life of the bearing plate 33 is prolonged. The silica gel heating plate is heated uniformly and the bottom surface is flexible, when the silica gel heating plate is abutted against the top cover 102 of the amplification container 101, the top cover 102 of the amplification containers 101 can be ensured to be fully contacted with the silica gel heating plate, the heat exchange efficiency is improved, and the uniformity of the temperature of each amplification container 101 is facilitated.
The opening and closing mechanism 2 includes a driving member 21, a rail member 22, a slider 23, and a floating assembly 24. The sliding member 23 is slidably connected to the track member 22 and the upper portion is connected to the bearing plate 33, the driving member 21 is simultaneously connected to the sliding member 23 and the outer wall of the second thermal insulation cabinet 12, and the floating assembly 24 is connected to the upper portion of the sliding member 23.
The track member 22 comprises two straight tracks 221 which are arranged in a flush manner, one ends of the two straight tracks 221 are respectively connected to two side surfaces of the outer wall of the second thermal insulation bin 12, and the two side surfaces are short sides of a rectangle. The other ends of the two linear rails 221 extend horizontally along the outer wall of the second thermal insulation cabinet 12.
The slider 23 comprises two sliding sides 231 and a connecting surface 232. The two sliding sides 231 are respectively slidably connected to the two linear rails 221 and are flush with each other, and the sliding sides 231 are parallel to the linear rails 221 and are vertically arranged. The connecting surface 232 is vertically connected to one end of the two sliding side surfaces 231 far away from the second insulation bin 12, and the bottom of the connecting surface 232 is higher than the top of the linear rail 221. The shape of the inner wall of the sliding part 23 is adapted to the outer wall of the second thermal insulation bin 12, when the sliding part 23 is positioned at one end of the track part 22 connected with the second thermal insulation bin 12, the sliding side 231 is flush with the side of the second thermal insulation bin 12, the connecting surface 232 is in conflict with the outer wall of the second thermal insulation bin 12, and at the moment, the sliding part 23 and the upper bearing plate 33 form a box-like structure, so that heat dissipation is reduced, energy conservation is facilitated, and the energy utilization rate is improved.
The float assembly 24 includes a float rod 241, a compression spring 242, and a sliding through hole 243, the float rod 241 is a vertically disposed cylindrical tube, and the top is configured as a stopper 2411 having a large radius. The floating rods 241 are disposed at four positions and are respectively disposed at two ends of the upper surfaces of the two sliding sides 231. A compression spring 242 is nested below the outside limit portion 2411 of the floating rod 241. The sliding through holes 243 are located at four corners of the heat cover 3, and the positions are adapted to the floating bars 241. The sliding through hole 243 passes through the bearing plate 33 and the heat insulating plate 32, but does not pass through the silica gel heating sheet 31. The aperture of the sliding through hole 243 is larger than the floating lever 241 and smaller than the stopper 2411. The heat cover 3 is slidably connected to the floating rod 241 through the sliding through hole 243 and supported above the compression spring 242, and is limited below the limiting portion 2411. When the nucleic acid amplification apparatus is heated, the silica gel heating sheet 31 under the heat cover 3 is brought into contact with the top cover 102 of the amplification vessel 101 by pressing down the carrier plate 33, thereby improving the heat exchange efficiency. After the temperature adjustment is finished, the external force is removed, and the thermal cover 3 returns to the upper side under the action of the compression spring 242. The silica gel heating sheet 31 is not in contact with the top cover 102 of the amplification vessel 101 any more, and no interaction occurs, so that the friction force between the silica gel heating sheet 31 and the top cover 102 of the amplification vessel 101 is prevented from obstructing the sliding of the slider 23.
The driving member 21 includes a motor 211 and a screw 212. The motor 211 is supported on the outer wall extending from the bottom of the second thermal insulation bin 12 and is fixed at the side of the second thermal insulation bin 12. The screw 212 is horizontally disposed between the two linear rails 221 and parallel to the two linear rails 221. The two fixed ends of the screw rod 212 are fixedly connected between the motor 211 and the second heat preservation, and the movable end is fixedly connected with the sliding piece 23. The motor 211 provides power, and the screw rod 212 plays a role in transmission, converts the force of axial rotation into the force of axial horizontal rotation, and drives the sliding piece 23 to slide along the linear track 221.
The nucleic acid amplification apparatus shown in this embodiment further includes a controller 5. The controller 5 is connected to the outer wall of the second thermal insulation bin 12 and is positioned on the long side far away from the opening and closing mechanism 2. The controller 5 is connected with the first temperature adjusting part 14, the temperature measuring part 15, the cooling fan 162, the motor 211 and the silica gel heating sheet 31. And regulating and controlling the working conditions of the components and parts, and displaying the temperature conditions and the working state of the nucleic acid amplification device. In this embodiment, the controller 5 is further connected with an external data line, so that it can exchange conductive data with external devices and systems.
The nucleic acid amplification apparatus of this embodiment is operated by comprising the steps of:
the motor 211 drives the screw rod 212 to rotate, so that the sliding piece 23 slides relative to the second thermal insulation bin 12 along the track piece 22, and drives the bearing plate 33 to be far away from the upper part of the second thermal insulation bin 12, thereby facilitating the placement of the amplification container 101 in the second thermal insulation bin 12;
after the amplification containers 101 are placed in the second pipe rack holes 121 on the second thermal insulation bin 12, the driving piece 21 drives the sliding piece 23 to move again so that the thermal cover 3 is positioned right above the second thermal insulation bin 12;
the bearing plate 33 slides downwards under the action of external force, the compression spring 242 compresses, the silica gel heating plate 31 and the first temperature adjusting piece 14 are heated simultaneously, and the heat dissipation fan 162 dissipates heat;
after the feedback temperature of the temperature regulating line reaches the maximum pre-denaturation temperature, the first temperature regulating piece 14 and the cooling fan 42 intermittently work, and the temperature is kept until the time reaches the pre-denaturation time and the denaturation time;
the first temperature adjusting piece 14 is refrigerated until the feedback temperature of the temperature adjusting line reaches the highest annealing temperature, and the first temperature adjusting piece 14 and the cooling fan 162 stop working; after the feedback temperature of the temperature regulating line is lower than the minimum annealing temperature, the first temperature regulating member 14 heats again, the cooling fan 162 is started again, and the intermittent operation is performed until the time reaches the annealing time;
heating to the extension temperature again, and repeating the steps for heat preservation;
the silica gel heating plate is closed, the external force applied above the bearing plate 33 is removed, the compression spring 242 is restored, the bearing plate 33 returns to the top of the floating rod 241, the driving piece 21 drives the sliding piece 23 to move again, the heat cover 3 is separated from the position right above the second heat preservation bin 12, and the first temperature adjusting piece 14 is refrigerated to the nucleic acid preservation temperature.
The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.
The above examples illustrate only a few embodiments of the utility model, which are described in detail and are not to be construed as limiting the scope of the utility model. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the utility model, which are all within the scope of the utility model. Accordingly, the scope of protection of the present utility model is to be determined by the appended claims.
Claims (10)
1. A nucleic acid amplification apparatus comprising;
the bottom temperature adjusting mechanism (1) is used for bearing a plurality of amplification containers (101) and adjusting the temperature of the bottoms of the amplification containers (101), and a top cover (102) is arranged at the top of each amplification container (101);
a thermal cover (3) for heating the top of the top cover (102) of all the amplification containers (101), the bottom of the thermal cover (3) being of a flexible structure;
the opening and closing mechanism (2) is arranged between the bottom temperature adjusting mechanism (1) and the thermal cover (3), so that the thermal cover (3) has freedom degrees in the transverse direction and the longitudinal direction, and the thermal cover (3) is far away from or props against the top cover (102) of the amplified container (101).
2. The nucleic acid amplification apparatus as set forth in claim 1, wherein the thermal cover (3) includes a silica gel heating sheet (31) provided at the bottom of the thermal cover (3) for abutting against and heating the top cover (102) of all the amplification containers (101);
the bearing plate (33) is arranged at the top of the thermal cover (3) and is used for connecting the opening and closing mechanism (2) and bearing the silica gel heating sheet (31).
3. The nucleic acid amplification apparatus according to claim 2, wherein the opening/closing mechanism (2) comprises:
the rail piece (22) comprises a plurality of linear rails (221) with the same direction, a first end of each linear rail (221) is connected with the bottom temperature regulating mechanism (1), and a second end of each linear rail (221) is far away from the bottom temperature regulating mechanism (1);
a sliding member (23) slidably connected to the linear rail (221), wherein the sliding member (23) is structurally adapted to the bottom temperature adjusting mechanism (1), and when the sliding member (23) reaches the first end of the linear rail (221), the sliding member (23) is nested outside the bottom temperature adjusting mechanism (1) and is in contact with the bottom temperature adjusting mechanism (1);
the two ends of the driving piece (21) are respectively connected with the sliding piece (23) and the bottom temperature adjusting mechanism (1), the driving piece (21) is parallel to the track piece (22), and the two ends of the driving piece extend in opposite directions and are used for driving the sliding piece (23) to slide along the track piece (22);
-a float assembly (24) connecting said slider (23) with said thermal cover (3) and providing said thermal cover (3) with a longitudinal degree of freedom.
4. A nucleic acid amplification apparatus according to claim 3, characterized in that the floating assembly (24) comprises a floating rod (241) vertically provided on top of the slider (23), a compression spring (242) nested outside the floating rod (241), and a sliding through hole (243) provided in the thermal cover (3) and adapted to the floating rod (241).
5. The nucleic acid amplification apparatus as set forth in claim 2, wherein the thermal cover (3) further comprises a heat shielding plate (32), the heat shielding plate (32) being disposed between the carrier plate (33) and the thermal cover (3).
6. The nucleic acid amplification apparatus as set forth in claim 1, wherein the bottom temperature adjustment mechanism (1) includes:
the second heat preservation bin (12) is constructed into a box-packed structure with a hollowed bottom, a plurality of second pipe support holes (121) are formed in the top of the second heat preservation bin (12), the amplification container (101) is erected above the second pipe support holes (121), and the inner wall of the second pipe support holes (121) is adapted to the outer wall of the amplification container (101);
the heat conductors (13) comprise a base (131) at the bottom and a plurality of heat conducting sleeves (132) above the base (131), the base (131) is directly or indirectly connected with the hollowed-out position at the bottom of the second thermal insulation bin (12) to form an inner space with the closed bottom, the position of each heat conducting sleeve (132) corresponds to the second pipe rack hole (121), the structure is suitable for the bottom structure of the amplification container (101), and when the amplification container (101) is erected above the second pipe rack hole (121), the outer wall of the bottom of the amplification container (101) is abutted against the inner wall of the heat conducting sleeve (132);
and a first temperature regulating member (14) positioned below the heat conductor (13) and in contact with a part of the bottom surface of the base (131) of the heat conductor (13).
7. The nucleic acid amplification apparatus as set forth in claim 6, wherein the bottom temperature adjustment mechanism (1) further comprises a temperature measuring member (15), the temperature measuring member (15) being provided on an inner wall of the heat conducting jacket (132) in contact with an outer wall of the bottom of the amplification vessel (101).
8. The nucleic acid amplification device according to claim 6, wherein the bottom temperature adjusting mechanism (1) further comprises a first thermal insulation bin (11), the first thermal insulation bin (11) is constructed into a box structure with a hollowed bottom, the first thermal insulation bin (11) is nested inside the second thermal insulation bin (12), the hollowed bottom of the first thermal insulation bin (11) is embedded with a base (131) of the heat conductor (13), the bottom of the first thermal insulation bin (11) is connected with the bottom of the second thermal insulation bin (12) to form a closed space, a plurality of first pipe rack holes (111) are formed in the top of the first thermal insulation bin (11), and the first pipe rack holes (111) are located between the thermal conduction sleeve (132) and the second pipe rack holes (121) and are adapted to the outer wall of the amplification container (101).
9. The nucleic acid amplification apparatus as set forth in claim 8, wherein the first temperature adjusting member (14) is a semiconductor cooling plate, a heat dissipating component (16) is connected to the bottom of the semiconductor cooling plate, and a heat insulating cavity (141) is formed between the upper surface of the heat dissipating component (16), the lower surface of the base (131) of the heat conductor (13), the side surface of the semiconductor cooling plate, and the inner wall of the first thermal insulation chamber (11).
10. The nucleic acid amplification apparatus as set forth in claim 9, wherein the heat dissipating assembly (16) includes a heat sink (161) connected to a bottom of the semiconductor cooling fin and a heat dissipating fan (162) connected to a bottom of the heat sink (161).
Priority Applications (1)
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CN202322267640.6U CN220767019U (en) | 2023-08-23 | 2023-08-23 | Nucleic acid amplification device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN202322267640.6U CN220767019U (en) | 2023-08-23 | 2023-08-23 | Nucleic acid amplification device |
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CN220767019U true CN220767019U (en) | 2024-04-12 |
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CN202322267640.6U Active CN220767019U (en) | 2023-08-23 | 2023-08-23 | Nucleic acid amplification device |
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