CN109059418B - Biological product cryopreservation and recovery device and cryopreservation and recovery method - Google Patents
Biological product cryopreservation and recovery device and cryopreservation and recovery method Download PDFInfo
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- CN109059418B CN109059418B CN201811077561.6A CN201811077561A CN109059418B CN 109059418 B CN109059418 B CN 109059418B CN 201811077561 A CN201811077561 A CN 201811077561A CN 109059418 B CN109059418 B CN 109059418B
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
- F25D31/00—Other cooling or freezing apparatus
- F25D31/005—Combined cooling and heating devices
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25B—REFRIGERATION MACHINES, PLANTS OR SYSTEMS; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS
- F25B21/00—Machines, plants or systems, using electric or magnetic effects
- F25B21/02—Machines, plants or systems, using electric or magnetic effects using Peltier effect; using Nernst-Ettinghausen effect
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25B—REFRIGERATION MACHINES, PLANTS OR SYSTEMS; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS
- F25B2321/00—Details of machines, plants or systems, using electric or magnetic effects
- F25B2321/02—Details of machines, plants or systems, using electric or magnetic effects using Peltier effects; using Nernst-Ettinghausen effects
- F25B2321/023—Mounting details thereof
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02B—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO BUILDINGS, e.g. HOUSING, HOUSE APPLIANCES OR RELATED END-USER APPLICATIONS
- Y02B30/00—Energy efficient heating, ventilation or air conditioning [HVAC]
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Abstract
The invention discloses a biological product cryopreservation and resuscitation device and a cryopreservation and resuscitation method. The invention comprises a symmetrically arranged elastic extrusion device, a sample supporting splint and a controller. The elastic extrusion device comprises a heat conduction clamping plate, a thrust plate, a slide bar fixing block and a temperature control device. The temperature control device adopts a superposed refrigeration technology, so that the freezing temperature of the biological product can reach an ultralow temperature state below minus 50 ℃. In the freezing and resuscitating process, the sample is continuously extruded, so that the temperature of the liquid biological product is reduced, and then extrusion is stopped, the product is frozen, the uniform cooling and freezing of the product are ensured, and the activity of the biological product is ensured.
Description
Technical Field
The invention relates to a refrigerating device in the field of medical appliances, in particular to a biological product freezing and recovering device and a freezing and recovering method.
Background
Because the freezing technology has better protection effect on biological characteristics at low temperature, the freezing technology is increasingly widely applied to biological medicines, foods, crops and the like. Two factors directly affecting the activity of biological agents, complex composition of components and spatial structure, so that the liquid biological agent is unstable and needs to be frozen into a solid state for storage for long-term storage or directly subjected to low-temperature sublimation and drying in the solid state. However, in the process of changing from a liquid state to a solid state, mechanical effects and solute effects are generated, so that the activity of the liquid is greatly influenced, and the concentration of the liquid in the liquid is higher and higher due to gradual freezing, so that the activity of cells is lost. It is therefore necessary to add some protective substances and to control the temperature process to influence its crystallization process, thus preserving its activity. But the additives also have a negative effect. In addition, the frozen solid state melts into a liquid state, and there are also mechanical effects and solute effects, so that rapid and uniform dissolution is required to avoid activity loss. It is therefore an urgent need to design a biological cryopreservation and resuscitation device that protects the activity of the biological product.
Disclosure of Invention
The invention aims to solve the technical problems that: provides a biological product freezing and recovering device and a freezing and recovering method for effectively keeping the activity of biological products.
The technical scheme for solving the technical problems is as follows: the utility model provides a biological product cryopreservation and resuscitator which characterized in that: the device comprises an elastic extrusion device, a sample supporting clamp plate and a controller, wherein the elastic extrusion device comprises a heat conduction clamp plate, a thrust plate, a slide bar fixing block and a temperature control device, the temperature control device comprises a supporting clamping seat, a compression plate, a heat exchanger, a secondary heat dissipation semiconductor refrigerating plate, a heat conduction plate and a primary temperature control semiconductor refrigerating plate, the supporting clamping seat comprises a bottom plate and a side plate arranged along the edge of the bottom plate, a refrigerating through hole is formed in the bottom plate, the primary temperature control semiconductor refrigerating plate is arranged at the upper part of the refrigerating through hole, the lower part of the primary temperature control semiconductor refrigerating plate is a refrigerating surface and is provided with a target temperature probe, the primary temperature control semiconductor refrigerating plate is used for cooling an article or space, the heat conduction plate is arranged at the upper part of the primary temperature control semiconductor refrigerating plate, the secondary heat dissipation semiconductor refrigerating plate is arranged at the upper part of the heat conduction plate, the lower part of the secondary heat dissipation semiconductor refrigerating plate is a refrigerating surface and is provided with a secondary temperature probe, the heat exchanger is arranged at the upper part of the secondary semiconductor refrigerating plate, and the heat exchanger is connected with the supporting clamp plate, the heat dissipation plate is electrically connected with the heat dissipation plate and the heat dissipation device, and the heat dissipation device is electrically connected with the heat dissipation device; the two side surfaces of the heat conducting clamping plate are respectively an extrusion side and a driving side, the middle part of the driving side of the heat conducting clamping plate is fixedly connected with a temperature control device, the heat conducting clamping plate is tightly attached to the refrigerating surface of a first-level temperature control semiconductor refrigerating sheet of the temperature control device, at least four slide bar fixing blocks are arranged, the slide bar fixing blocks are fixedly connected with the driving side of the heat conducting clamping plate and uniformly distributed on the periphery of the temperature control device, the thrust plate is provided with bearing fixing holes with the same quantity as that of the slide bar fixing blocks, the axial leads of the bearing fixing holes and the slide bar are overlapped, a linear bearing is arranged in the bearing fixing holes, one end of the slide bar is connected with the slide bar fixing blocks, the other end of the slide bar passes through the linear bearing and is provided with an anti-falling block, two ends of each slide bar are sleeved with a telescopic spring, and the two ends of each telescopic spring are respectively abutted with the thrust plate and the heat conducting clamping plate, the thrust plate is kept away from one side of heat conduction splint and is equipped with flange, be equipped with the screw on the flange, sample support splint is equipped with two, is equipped with connecting device between two sample support splints, sample support splint is located between two elastic extrusion devices, be equipped with temperature probe on the heat conduction splint, temperature probe, temperature control device and controller electrical connection, be equipped with soft sheath between two heat conduction splints, soft sheath and two heat conduction splints enclose into the open cavity in upper portion, and the both sides of soft sheath are laminated with the edge of the drive side one side of two heat conduction splints respectively, elastic extrusion device is equipped with the shell, the shell is the tubulose, the one end and the slide bar fixed block fixed connection of shell to the tip of shell and the soft sheath butt of laminating in heat conduction splint drive side one side.
Preferably, the middle part of the driving side of the heat conducting clamping plate is provided with a bulge for being embedded into a refrigerating through hole of the supporting clamping seat,
the connecting flange is arranged in the middle of the thrust plate.
Preferably, the heat exchanger comprises a heat exchanger shell made of heat conducting materials and a runner arranged inside the heat exchanger shell, and an inlet and an outlet of the runner are arranged on the heat exchanger shell.
Preferably, the sample supporting splint is a frame, and each side of the sample supporting splint is flat.
Preferably, the connecting device of the two sample supporting clamping plates is a screw, and the sample supporting clamping plates are provided with screw holes.
Preferably, the connecting device of the two sample supporting splints comprises a hasp fixing piece and a hasp, and the two sample supporting splints are respectively provided with the hasp fixing piece and the hasp.
Preferably, a ring piece fixedly connected with the edge of the shell is arranged in the shell near one end of the heat conduction clamping plate.
Better, still include mounting fixture, mounting fixture includes the base, be equipped with the spliced pole of two perpendicular to bases on the base, the upper portion of spliced pole is equipped with the screw, the inside butterfly bolt that is equipped with of screw, the tip that the butterfly bolt is located between two spliced poles is equipped with the contact plate, the plane center and the butterfly bolt rotation of contact plate are connected, the contact plate surface is equipped with the rubber pad, be equipped with the screw on the contact plate, the screw matches with the screw on the flange for with flange spiro union connection.
Preferably, the soft sheath is elongated and has a cross-section
The edge of the soft sheath is clamped between the heat conducting clamping plate and the shell.
The application method of the biological product cryopreservation and resuscitation device is characterized by comprising the following steps of:
sample freezing process:
step 1.1, separating two sample support splints, placing a bag of a sample to be frozen in the middle of the sample support splints, clamping edges of the bag by the two sample support splints, locking the two sample support splints,
step 1.2, inserting a sample supporting splint clamped with a sample between two elastic extrusion devices, then injecting antifreeze fluid into a cavity enclosed by the soft sheath and the two heat conducting splints,
step 1.3, connecting a connecting flange of the thrust plate with an external reciprocating mechanism or with a swinging mechanism, and enabling the sample to be tightly attached to the heat conducting clamping plate so that a bag containing the sample is in a flat shape in a vertical state,
step 1.4, starting an external reciprocating mechanism or a swinging mechanism to enable the sample solution to be in a dynamic convection state,
step 1.5, starting a controller to refrigerate the sample, and controlling the temperature of the heat conduction clamping plate to be within 5 ℃ under the range of eutectic points, so that the sample is in a liquid supercooled state at the crystallization temperature;
step 1.6, closing an external reciprocating mechanism or a swinging mechanism to freeze the sample, wherein the method specifically comprises the following steps: the temperature of the heat conduction clamping plate is controlled to be rapidly reduced to 10-20 ℃ lower than the range of the eutectic point, so that the sample is rapidly and uniformly crystallized, and then the controller is closed;
step 1.7, taking out the frozen sample, firstly taking out the sample supporting clamping plate from between the two heat conducting clamping plates, and then opening the sample supporting clamping plate to take out the sample;
sample recovery process:
step 2.1, separating the two sample support clamping plates, placing the bag of the frozen sample in the middle of the sample support clamping plates, clamping the edges of the bag by the two sample support clamping plates, locking the two sample support clamping plates,
step 2.2, inserting a sample supporting clamping plate clamped with a sample between two elastic extrusion devices, and then injecting antifreeze fluid into a cavity surrounded by the soft jacket and the two heat conducting clamping plates;
step 2.3, connecting the connecting flange of the thrust plate with an external reciprocating mechanism or with a swinging mechanism, and enabling the frozen sample to be closely attached to the heat conducting clamping plate,
step 2.4, starting an external reciprocating mechanism or a swinging mechanism,
step 2.5, starting the controller, setting the temperature of the heat conducting clamping plate, controlling the temperature control device to keep constant set temperature,
step 2.6, detecting the temperature of the sample, stopping the external reciprocating mechanism or the swinging mechanism and stopping the controller after the frozen sample is restored to the set temperature;
step 2.7, taking out a sample after thawing and resuscitating, wherein the sample specifically comprises the following components: the sample support clamping plate is taken out from between the two heat conduction clamping plates, and then the sample support clamping plate is opened to take out the sample.
The beneficial effects of the invention are as follows:
1. has the beneficial effect of effectively keeping the activity of biological products in the freezing and resuscitating process;
2. rapidly cooling and reaching ultra-low temperature below-50 ℃;
3. has the function of convenient operation and has simple requirements on external driving equipment.
Drawings
Figure 1 is a schematic diagram of one embodiment of the present invention,
figure 2 is a schematic view of a fixture according to one embodiment of the invention,
figure 3 is a schematic view of a temperature control device according to one embodiment of the present invention,
figure 4 is a schematic view of the appearance of an embodiment of the invention,
FIG. 5 is a schematic view of a sample support splint according to one embodiment of the present invention.
In the figure:
84. a contact plate;
83. a butterfly bolt;
82. a clamping column;
81. a base;
24. a second-stage temperature probe;
23. a target temperature probe;
218. a first-stage temperature-control semiconductor refrigerating sheet;
216. a heat conductive sheet;
215. a second-stage heat-dissipating semiconductor refrigerating sheet;
214. a heat exchanger;
213. a compacting plate;
212. a supporting clamping seat;
2. a temperature control device;
4. a slide bar fixing block;
3. a slide bar;
7. a thrust plate;
1. a heat conductive clamping plate;
10. a sample support splint;
Detailed Description
In order to make the technical scheme and beneficial effects of the present invention clearer, the following further explain the embodiments of the present invention in detail.
As shown in fig. 1, a biological product cryopreservation and resuscitating device comprises a symmetrically arranged elastic pressing means, a sample supporting splint 10 and a controller.
The elastic extrusion device is used for extruding the bagged samples in the cooling process, keeping the samples in a liquid state for cooling, and stopping extruding for freezing. The elastic extrusion device comprises a heat conduction clamping plate 1, a thrust plate 7, a slide bar 3, a slide bar fixing block 4 and a temperature control device 2. The temperature control device 2 is used for cooling the heat conduction clamping plate 1, and the heat conduction clamping plate 1 is used for extruding a sample and cooling the sample. The thrust plate 7 is used for connecting an external driving mechanism and transmitting power to the heat conducting clamping plate 1 through buffering.
Better, in order to achieve the effects of heat conduction, corrosion resistance and good stability, the heat conduction clamping plate 1 is made of 304 stainless steel or 316L stainless steel.
The temperature control device 2 is used for cooling objects and a refrigerating space, and comprises a temperature control housing 211, a supporting clamping seat 212, a pressing plate 213, a heat exchanger 214, a secondary heat dissipation semiconductor refrigerating sheet 215, a heat conducting sheet 216, a connecting terminal and a primary temperature control semiconductor refrigerating sheet 218. The temperature control housing 211 and the support holder 212 wrap the compression plate 213, the heat exchanger 214, the secondary heat dissipation semiconductor refrigeration sheet 215, the heat conduction sheet 216, and the primary temperature control semiconductor refrigeration sheet 218 inside, and fill the empty space with heat insulation materials. The wiring terminal is used for connecting an internal component and an external controller.
The support holder 212 includes a bottom plate and a side plate disposed along an edge of the bottom plate, wherein the side plate is perpendicular to the bottom plate. In order to facilitate the first-level temperature-controlled semiconductor 218 to cool down an object or a refrigerated space, a refrigeration through-hole is provided in the bottom plate. A first-stage temperature-controlled semiconductor cooling fin 218 is provided at the upper portion of the cooling through hole. The primary temperature-controlled semiconductor cooling fin 218 is a semiconductor cooling fin, or called an electric cooling fin, and has two cooling surfaces and a heat dissipating surface. Preferably, the area of the primary temperature-controlled semiconductor cooling fin 218 is greater than the area of the cooling through hole. The method has the beneficial effects that: firstly, the heating surface can be prevented from radiating heat to one side of the support card seat 212; and secondly, the first-stage temperature-control semiconductor refrigerating sheet 218 can be supported so as to be in close contact with other components in a compressed state. The transfer of heat from the cooling surface to the heat dissipating surface is achieved by applying direct current to the primary temperature-controlled semiconductor 218, thereby achieving refrigeration of the object or refrigerated space. In order to facilitate detection of the temperature of the cooling surface of the primary temperature-controlled semiconductor cooling fin 218, a target temperature probe 223 is provided on the cooling surface of the lower portion of the primary temperature-controlled semiconductor cooling fin 218.
When the temperature difference between the cooling surface and the radiating surface of the primary temperature control semiconductor 218 reaches a certain temperature, a refrigerating limit is generated, that is, the heat of the radiating surface is diffused to the cooling surface, so that the refrigerating effect is reduced. To solve this limitation, it is necessary to cool the heat radiation surface, and therefore, a heat radiation device is provided at the upper portion of the primary temperature-controlled semiconductor cooling fin 218. The heat sink includes a heat conductive sheet 216 and a secondary heat dissipating semiconductor cooling sheet 215. Preferably, in order to prevent the heat of the heat dissipation surface of the first-stage temperature-controlled semiconductor cooling fin 218 from being diffused, the area of the heat conduction fin 216 is larger than that of the first-stage temperature-controlled semiconductor cooling fin 218. The heat conductive sheet 216 is disposed on the upper portion of the primary temperature-controlled semiconductor cooling sheet 218, the secondary heat dissipation semiconductor cooling sheet 215 is disposed on the upper portion of the heat conductive sheet, and the area of the secondary heat dissipation semiconductor cooling sheet 215 is larger than the area of the primary temperature-controlled semiconductor cooling sheet 218. In the prior art, the semiconductor refrigeration piece has a smaller area, if the large area cannot be obtained due to the process limitation, the secondary heat dissipation semiconductor refrigeration piece 215 can be realized by adopting a mode of splicing a plurality of semiconductor refrigeration pieces. Also, in order to detect the temperature, a secondary temperature probe 24 is provided on the lower cooling surface of the secondary heat dissipation semiconductor cooling plate 215 to detect the temperature of the cooling surface of the secondary heat dissipation semiconductor cooling plate 215.
Preferably, both sides of the heat conductive sheet 216 are coated with heat conductive silicone grease to increase the heat transfer effect between the primary temperature-controlled semiconductor cooling sheet 218 and the secondary heat-dissipating semiconductor cooling sheet 215.
More preferably, in order to enhance the refrigerating effect, a tertiary heat dissipation semiconductor refrigerating sheet and a second heat conduction sheet are provided between the heat exchanger 214 and the secondary heat dissipation semiconductor refrigerating sheet 215. The heat exchanger 214 is in contact with the heating surface of the third-stage heat dissipation semiconductor refrigeration sheet, two sides of the second heat conduction sheet are respectively in contact with the second-stage heat dissipation semiconductor refrigeration sheet 215 and the third-stage heat dissipation semiconductor refrigeration sheet, and two sides of the heat conduction sheet 216 and the second heat conduction sheet are coated with heat conduction silicone grease to increase the heat conduction effect.
A heat exchanger is a device that transfers a portion of the heat of a hot fluid to a cold fluid, also known as a heat exchanger. The heat exchanger plays an important role in chemical industry, petroleum, power, food and other industrial production, and can be used as a heater, a cooler, a condenser, an evaporator, a reboiler and the like in the chemical industry, so that the heat exchanger has wide application range. The heat exchanger 214 in the present invention includes a heat exchanger housing made of a heat conductive material, a flow passage provided inside the heat exchanger housing, and an inlet and an outlet of the flow passage provided on the heat exchanger housing. The circulation is made by providing a partition perpendicular to the upper and lower surfaces between the upper and lower surfaces inside the housing, and the internal flow channels may be provided as serpentine flow channels or as parallel flow channels depending on the manner in which the partition is arranged. The serpentine flow passage is an inlet and passes through the serpentine flow passage to an outlet. The parallel flow channels are inlet inlets, then enter each parallel flow channel respectively, and finally the parallel flow channels are converged into the outlet. The inlet and outlet of the heat exchanger 214 are connected to an external cold source, typically cold water, cold alcohol, etc.
The two sides of the heat conduction clamping plate 1 are respectively an extrusion side and a driving side, the extrusion side is used for extruding a sample, and the driving side is used for connecting the thrust plate 7. One of the functions of the heat conducting clamping plate 1 is refrigeration, so that the driving side of the heat conducting clamping plate 1 is fixedly connected with the temperature control device 2, and the heat conducting clamping plate 1 is in contact with the refrigeration surface of the temperature control device 2. In order to realize the tight connection with the refrigeration surface of the temperature control device 2 so as to increase the cold energy conduction effect, a bulge is arranged in the middle of the driving side of the heat conduction clamping plate 2, and the bulge in the middle is the same as the refrigeration through hole of the temperature control device 2 in shape, and the bulge is embedded into the refrigeration through hole so as to realize the tight fit of the heat conduction clamping plate and the refrigeration surface of the first-stage temperature control semiconductor refrigeration sheet 218.
In order to realize the connection and disconnection of the sliding rod 3 and the heat conducting clamping plate 1, a sliding rod fixing block 4 is arranged on the driving side of the heat conducting clamping plate. The slide bar fixing block 4 is a rod-shaped object fixing device, and a novel curtain slide bar fixing device with the application number of 200320122864.8 is a slide bar fixing device. In the invention, the slide bar fixing block 4 is fixedly connected with the heat conducting clamping plate 1 and is detachably connected with the slide bar 3, but the connection of the slide bar and the slide bar fixing block 4 can be realized in a fixed connection mode. In order to reduce the loss of cold energy, the slide bar fixing block 4 is made of a material with low heat conductivity coefficient. In order to ensure uniform stress, the slide bar fixing blocks 4 are uniformly distributed on the edges of the side surface of the driving side of the heat conducting clamping plate 1. And more preferably, the heat conducting clamping plates are uniformly distributed around the middle bulge and positioned at the edge of the heat conducting clamping plate 1. The slide bar 3 is vertical to the heat conducting clamping plate 1 and is connected with the slide bar fixing block 4. In order to buffer the impact force of the external driving device, a telescopic spring 6 is sleeved on the sliding rod 3. The two ends of the telescopic spring 6 are respectively abutted against the thrust plate 7 and the driving side of the heat conduction clamping plate 1. The extension spring 6 serves to buffer the pressing force of the external driving device. Another advantage of installing the extension spring 6 is that in case of releasing the external driving force, the vibration state can be maintained for a while, thus reducing disturbance caused by unstable external driving force.
The thrust plate 7 is adapted to receive an external driving force, and is therefore provided with a connection flange on the side of the thrust plate remote from the heat-conducting clamping plate 1. In order to realize better connection with external driving equipment, the connecting flange is provided with screw holes. In order to realize the buffer effect of the telescopic spring 6, the thrust plate 7 needs to realize the relative motion with the slide bar 3, so that the thrust plate 7 is provided with bearing fixing holes, the number of the bearing fixing holes is the same as that of the slide bar 3 and the slide bar fixing block 4, and the axial leads of the bearing fixing holes and the slide bar 3 are coincident. The inside of the bearing fixing hole is provided with a linear bearing 5. The linear bearing 5 is sleeved at one end of the sliding rod 3 far away from the heat conduction clamping plate 1, and the linear bearing 5 is fixedly connected with the inner wall of the bearing fixing hole. In order to prevent the thrust plate 7 from slipping off, an anti-slip block 31 is provided at the end of the slide bar remote from the free end of the heat conducting clamping plate 1.
The extrusion sides of the heat conducting clamping plates 1 of the two elastic extrusion devices are opposite, and an extrusion and refrigeration space is formed between the two extrusion sides. The sample support clamp 10 is used to hold a sample and is placed in the middle of two elastic pressing means. The sample support splint 10 is provided with two. The sample supporting clamping plates 10 are of frame-shaped structures, and the two sample supporting clamping plates 10 are matched to clamp a sample, so that the two sample supporting clamping plates 10 are provided with connecting devices. In order to detect whether the temperature of the surfaces of the two heat conducting clamping plates 1 is uniform, temperature probes are arranged on the heat conducting clamping plates 1, and the temperature probes are arranged on a plurality of extrusion sides of the heat conducting clamping plates 1 and uniformly distributed. The temperature probe, the temperature control device and the controller are electrically connected.
Preferably, the connecting device of the two sample supporting splints 10 is a screw, and the sample supporting splints 10 are provided with screw holes. The combination of the two sample supporting splints 10 is achieved by the cooperation of screws and screw holes.
Preferably, the means for connecting the two sample support splints 10 comprises a snap fastener and a snap. The two sample supporting splints 10 are respectively provided with a snap fastener and a snap fastener. The fastening of the two sample support splints 10 is achieved by a quick connection of the clasp and clasp mount.
Preferably, the invention further comprises a fixing clamp for facilitating connection and placement. The fixing jig includes a base 81, and two clamping posts 82 provided on the base 81. The clamping post 82 is perpendicular to the base 81. The upper portion of the clamping column 82 is provided with a screw hole, a butterfly bolt 83 is arranged in the screw hole, a contact plate 84 is arranged at the end of the butterfly bolt 83, and the center of the contact plate 84 on the plane is rotationally connected with the butterfly bolt 83. Preferably, the contact plate 84 is provided with a through hole or a screw hole for screwing with a screw hole on the connecting flange of the thrust plate 7. Because the telescopic spring 6 is arranged in the invention, after the invention is fixed by the rack, the rack is fixed on the linear reciprocating platform, so that the heat conduction clamping plate 1 can squeeze the equipment.
Better, in order to realize the protection to the sample, prevent the sample from bonding with the heat conduction clamping plates in the freezing process, a soft sheath 9 is arranged between the two heat conduction clamping plates 1. The soft sheath 9 is arranged at the left and right sides and the lower part of the two heat conducting clamping plates 1. Two sides of the soft jacket 9 are respectively attached to edges of the driving side surfaces of the two heat-conducting clamping plates 1, and the soft jacket 9 and the two heat-conducting clamping plates 1 enclose a cavity with an open upper part. The cavity is used to house a sample support splint 10. The two sides of the soft sheath 9 are respectively inserted into the gaps between the heat conducting clamping plates 1 and the shell 8 at the left side and the right side.
Preferably, the soft sheath 9 is strip-shaped and has an pi-shaped cross section, and the soft sheath 9 is made of soft materials such as silica gel, PVC, PE and the like. The soft jacket 9 and the two heat conduction clamping plates 1 enclose a cavity with an open upper part, low-temperature antifreeze fluid can be injected into the cavity, the heat conduction clamping plates 1 and the sample are prevented from being adhered after being frozen when the sample is clamped, and meanwhile, heat transfer media are added, so that the heat transfer efficiency is improved.
Preferably, in order to ensure the operation safety of the equipment and prevent the clamping injury of workers, the invention also provides a shell 8. The shell 8 is sleeved outside the elastic extrusion device, the shell 8 is tubular, and one end of the shell 8 is fixedly connected with the slide bar fixing block 4. A gap is arranged between the end part of the shell 8 and the heat conducting clamping plate 1, and the gap is used for clamping the soft sheath 9, namely, the shell 8 extrudes the soft sheath 9 attached to one surface of the driving side of the heat conducting clamping plate 1 to the driving side of the heat conducting clamping plate 1 so as to realize connection and sealing with the soft sheath 9. Preferably, for easy connection, the end of the housing 8 connected to the slide bar fixing block 4 is provided with a ring piece which is perpendicular to the side wall of the housing and fixedly connected to the end of the housing.
Based on the structure, the method for freezing and resuscitating biological products comprises the following steps:
sample freezing process:
step 1.1, separating the two sample support splints 10, placing the bag of the sample to be frozen in the middle of the sample support splints 10, clamping the edges of the bag by the two sample support splints 10, locking the two sample support splints 10,
step 1.2, inserting a sample supporting splint 10 with a sample between two elastic extrusion devices, then injecting antifreeze fluid into a cavity enclosed by the soft sheath 9 and the two heat conducting splints 1,
step 1.3, connecting a connecting flange of the thrust plate 7 with an external reciprocating mechanism or with a swinging mechanism, and enabling the sample to be tightly attached to the heat conducting clamping plate 1 so that a bag containing the sample is in a flat shape in a vertical state,
step 1.4, starting an external reciprocating mechanism or a swinging mechanism to enable the sample solution to be in a dynamic convection state,
step 1.5, starting a controller to refrigerate a sample, and controlling the temperature of the heat conduction clamping plate 1 to be within 5 ℃ under the range of eutectic points so that the sample is in a liquid supercooled state under the crystallization temperature;
step 1.6, closing an external reciprocating mechanism or a swinging mechanism to freeze the sample, wherein the method specifically comprises the following steps: the temperature of the heat conduction clamping plate 1 is controlled to be rapidly reduced to 10-20 ℃ lower than the eutectic point range, so that a sample is rapidly and uniformly crystallized, and then the controller is closed;
step 1.7, taking out the frozen sample, firstly taking out the sample supporting clamping plate 10 from between the two heat conducting clamping plates 1, and then opening the sample supporting clamping plate 10 to take out the sample;
sample recovery process:
step 2.1, separating the two sample support splints 10, placing the bag of the frozen sample in the middle of the sample support splints 10, clamping the edges of the bag by the two sample support splints 10, locking the two sample support splints 10,
step 2.2, inserting a sample supporting splint 10 with a sample between two elastic extrusion devices, and then injecting antifreeze fluid into a cavity enclosed by the soft sheath 9 and the two heat conducting splints 1;
step 2.3, connecting the connecting flange of the thrust plate 7 with an external reciprocating mechanism or with a swinging mechanism, and enabling the frozen sample to be closely attached to the heat conducting clamping plate 1,
step 2.4, starting an external reciprocating mechanism or a swinging mechanism,
step 2.5, starting the controller, setting the temperature of the heat conducting clamping plate 1, controlling the temperature control device 2 to keep constant set temperature,
step 2.6, detecting the temperature of the sample, stopping the external reciprocating mechanism or the swinging mechanism and stopping the controller after the frozen sample is restored to the set temperature;
step 2.7, taking out a sample after thawing and resuscitating, wherein the sample specifically comprises the following components: the sample support splint 10 is first removed from between the two heat transfer splints 1, and then the sample support splint 10 is opened to remove the sample.
Because the external clamp clamps the sample by using the heat conduction clamping plate through the spring, the heat transfer area between the heat conduction clamping plate and the sample is ensured to be the maximum contact area. The external clamp moves to enable the heat conduction clamping plate to extrude the product, so that convection is formed inside the product, heat exchange is facilitated, and the whole sample is quickly dissolved and resuscitated. In the freezing process, the sample can be reduced to a lower temperature or even reach the freezing temperature in the liquid state, and after the external power is lost, the sample temperature is uniform, the freezing effect is good, and the activity of the product is ensured.
In summary, the above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention, and the related workers can make various changes and modifications without departing from the scope of the technical idea of the present invention. The technical scope of the present invention is not limited to the description, but includes all equivalent changes and modifications in shape, construction, characteristics and spirit according to the scope of the claims.
Claims (9)
1. The utility model provides a biological product cryopreservation and resuscitator which characterized in that:
comprises elastic extrusion devices which are symmetrically arranged, a sample supporting splint (10) and a controller,
the elastic extrusion device comprises a heat conduction clamping plate (1), a thrust plate (7), a slide bar (3), a slide bar fixing block (4) and a temperature control device (2),
the temperature control device (2) comprises a supporting clamping seat (212), a compacting plate (213), a heat exchanger (214), a secondary heat dissipation semiconductor refrigerating sheet (215), a heat conducting sheet (216) and a primary temperature control semiconductor refrigerating sheet (218),
the supporting clamping seat (212) comprises a bottom plate and a side plate arranged along the edge of the bottom plate, the bottom plate is provided with a refrigeration through hole,
the primary temperature control semiconductor refrigerating sheet (218) is arranged at the upper part of the refrigerating through hole, the lower part of the primary temperature control semiconductor refrigerating sheet (218) is a refrigerating surface, a target temperature probe (23) is arranged on the refrigerating surface, the primary temperature control semiconductor refrigerating sheet (218) is used for cooling objects or spaces,
the heat conducting fin (216) is arranged at the upper part of the primary temperature control semiconductor refrigerating fin (218),
the secondary heat dissipation semiconductor refrigerating sheet (215) is arranged at the upper part of the heat conducting sheet (216), the lower part of the secondary heat dissipation semiconductor refrigerating sheet (215) is a refrigerating surface, a secondary temperature probe (24) is arranged on the refrigerating surface,
the heat exchanger (214) is arranged at the upper part of the two-stage radiating semiconductor refrigerating plate (215),
the compressing plate (213) is arranged at the upper part of the heat exchanger (214), the compressing plate (213) is connected with the bottom plate of the supporting clamping seat (212),
the first-stage temperature control semiconductor refrigerating sheet (218), the second-stage heat dissipation semiconductor refrigerating sheet (215), the second-stage temperature probe (24) and the target temperature probe (23) are electrically connected with the controller;
the two side surfaces of the heat conduction clamping plate (1) are respectively an extrusion side and a driving side,
the middle part of the driving side of the heat conduction clamping plate (1) is fixedly connected with the temperature control device (2), the heat conduction clamping plate (1) is tightly attached to the refrigerating surface of the first-stage temperature control semiconductor refrigerating sheet (218) of the temperature control device (2),
the sliding rod fixing blocks (4) are at least provided with four, the sliding rod fixing blocks (4) are fixedly connected with the driving side of the heat conduction clamping plate (1) and are uniformly distributed around the temperature control device (2),
the thrust plate (7) is provided with bearing fixing holes with the same number as the slide bar fixing blocks (4), the bearing fixing holes are coincided with the axial lead of the slide bar (3), the inside of the bearing fixing holes is provided with a linear bearing (5),
one end of the sliding rod (3) is connected with the sliding rod fixing block (4), the other end of the sliding rod passes through the linear bearing (5) and the end part of the sliding rod is provided with an anti-falling block (31),
the slide bar (3) is sleeved with a telescopic spring (6), two ends of the telescopic spring (6) are respectively abutted with the thrust plate (7) and the heat conduction clamping plate (1),
a connecting flange is arranged on one side, far away from the heat conduction clamping plate (1), of the thrust plate (7), and is provided with a screw hole, so that the connecting flange of the thrust plate (7) of the elastic extrusion device is connected with an external reciprocating mechanism or a swinging mechanism;
the two sample supporting splints (10) are arranged, a connecting device is arranged between the two sample supporting splints (10), the sample supporting splints (10) are arranged between the two elastic extrusion devices,
a temperature probe is arranged on the heat conduction clamping plate (1), the temperature probe, the temperature control device and the controller are electrically connected,
a soft sheath (9) is arranged between the two heat-conducting clamping plates (1), the soft sheath (9) and the two heat-conducting clamping plates (1) enclose a cavity with an open upper part, two sides of the soft sheath (9) are respectively attached to the edges of one sides of the driving sides of the two heat-conducting clamping plates (1),
the elastic extrusion device is provided with a shell (8), the shell (8) is tubular, one end of the shell (8) is fixedly connected with the slide bar fixing block (4), and the end of the shell (8) is abutted with a soft sheath (9) attached to one surface of the driving side of the heat conducting clamping plate (1); the inside of the end of the shell (8) close to the heat conduction clamping plate (1) is provided with a ring piece fixedly connected with the edge of the shell (8).
2. The biologic cryopreservation and resuscitation apparatus of claim 1, wherein:
a bulge is arranged in the middle of the driving side of the heat conduction clamping plate (1) and is used for being embedded into a refrigeration through hole of the supporting clamping seat (212),
the connecting flange is arranged in the middle of the thrust plate (7).
3. The biologic cryopreservation and resuscitation apparatus of claim 1, wherein:
the heat exchanger (214) comprises a heat exchanger shell made of heat conducting materials and a runner arranged inside the heat exchanger shell, and an inlet and an outlet of the runner are arranged on the heat exchanger shell.
4. The biologic cryopreservation and resuscitation apparatus of claim 1, wherein:
the sample supporting clamp plate (10) is a frame body, and each side of the sample supporting clamp plate (10) is flat.
5. The biologic cryopreservation and resuscitation apparatus of claim 1, wherein:
the connecting device of the two sample supporting splints (10) is a screw, and screw holes are formed in the sample supporting splints (10).
6. The biologic cryopreservation and resuscitation apparatus of claim 1, wherein:
the connecting device of the two sample supporting splints (10) comprises a hasp fixing piece and a hasp, and the hasp fixing piece and the hasp are respectively arranged on the two sample supporting splints (10).
7. The biologic cryopreservation and resuscitation apparatus of claim 1, wherein:
still include mounting fixture, mounting fixture includes base (81), be equipped with clamping column (82) of two perpendicular to base (81) on base (81), the upper portion of clamping column (82) is equipped with the screw, the inside butterfly bolt (83) that is equipped with of screw, the tip that butterfly bolt (83) are located between two clamping column (82) is equipped with contact plate (84), the plane center and the butterfly bolt (83) of contact plate (84) rotate to be connected, contact plate (84) surface is equipped with the rubber pad, be equipped with the screw on contact plate (84), the screw matches with the screw on the flange for with flange spiro union connection.
8. The biologic cryopreservation and resuscitation apparatus of claim 1, wherein:
the soft sheath (9) is long-strip-shaped, the section of the soft sheath is pi-shaped or C-shaped, and the edge of the soft sheath (9) is clamped between the heat conducting clamping plate (1) and the shell (8).
9. A method of using a biologic cryopreservation and resuscitation apparatus according to claim 3, wherein:
sample freezing process:
step 1.1, separating two sample support splints (10), placing a bag of a sample to be frozen in the middle of the sample support splints (10), clamping edges of the bag by the two sample support splints (10), locking the two sample support splints (10),
step 1.2, inserting a sample supporting splint (10) with a sample between two elastic extrusion devices, then injecting antifreeze fluid into a cavity enclosed by the soft jacket (9) and the two heat conducting splints (1),
step 1.3, connecting a connecting flange of the thrust plate (7) with an external reciprocating mechanism or with a swinging mechanism, and tightly attaching the sample to the heat conducting clamping plate (1) so that a bag containing the sample is in a flat shape in a vertical state,
step 1.4, starting an external reciprocating mechanism or a swinging mechanism to enable the sample solution to be in a dynamic convection state,
step 1.5, starting a controller to refrigerate a sample, and controlling the temperature of the heat conduction clamping plate (1) to be within 5 ℃ under the range of eutectic points so that the sample is in a liquid supercooled state under the crystallization temperature;
step 1.6, closing an external reciprocating mechanism or a swinging mechanism to freeze the sample, wherein the method specifically comprises the following steps: the temperature of the heat conduction clamping plate (1) is controlled to be rapidly reduced to 10-20 ℃ lower than the eutectic point range, so that a sample is rapidly and uniformly crystallized, and then the controller is closed;
step 1.7, taking out the frozen sample, firstly taking out the sample supporting clamp plate (10) from between the two heat conducting clamp plates (1), and then opening the sample supporting clamp plate (10) to take out the sample;
sample recovery process:
step 2.1, separating the two sample support splints (10), placing the bag of the frozen sample in the middle of the sample support splints (10), clamping the edges of the bag by the two sample support splints (10), locking the two sample support splints (10),
step 2.2, inserting a sample supporting clamp plate (10) with a sample clamped between two elastic extrusion devices, and then injecting antifreeze fluid into a cavity enclosed by the soft jacket (9) and the two heat conducting clamp plates (1);
step 2.3, connecting a connecting flange of the thrust plate (7) with an external reciprocating mechanism or with a swinging mechanism, and enabling the frozen sample to be closely attached to the heat conducting clamping plate (1),
step 2.4, starting an external reciprocating mechanism or a swinging mechanism,
step 2.5, starting a controller, setting the temperature of the heat conducting clamping plate (1), controlling the temperature control device (2) to keep constant set temperature,
step 2.6, detecting the temperature of the sample, stopping the external reciprocating mechanism or the swinging mechanism and stopping the controller after the frozen sample is restored to the set temperature;
step 2.7, taking out a sample after thawing and resuscitating, wherein the sample specifically comprises the following components: the sample supporting clamp plate (10) is firstly taken out from the space between the two heat conducting clamp plates (1), and then the sample supporting clamp plate (10) is opened to take out the sample.
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