CN110172400A - A kind of apparatus for the anhydrous dry type recovery of cell - Google Patents
A kind of apparatus for the anhydrous dry type recovery of cell Download PDFInfo
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- CN110172400A CN110172400A CN201910564380.4A CN201910564380A CN110172400A CN 110172400 A CN110172400 A CN 110172400A CN 201910564380 A CN201910564380 A CN 201910564380A CN 110172400 A CN110172400 A CN 110172400A
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- rewarming
- double function
- recovery
- pallets
- cell
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- 238000011084 recovery Methods 0.000 title claims abstract description 172
- 238000005138 cryopreservation Methods 0.000 claims abstract description 137
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims abstract description 38
- 238000010438 heat treatment Methods 0.000 claims description 59
- 238000012544 monitoring process Methods 0.000 claims description 25
- 230000033001 locomotion Effects 0.000 claims description 22
- 230000005855 radiation Effects 0.000 claims description 22
- 230000010355 oscillation Effects 0.000 claims description 13
- 230000000694 effects Effects 0.000 claims description 8
- 238000004891 communication Methods 0.000 claims description 7
- 238000001514 detection method Methods 0.000 claims description 7
- 229910052751 metal Inorganic materials 0.000 claims description 7
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- 238000000034 method Methods 0.000 claims description 5
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- 238000011010 flushing procedure Methods 0.000 claims description 3
- 238000007710 freezing Methods 0.000 claims description 3
- 230000008014 freezing Effects 0.000 claims description 3
- 238000009434 installation Methods 0.000 claims description 3
- 230000003139 buffering effect Effects 0.000 claims 1
- 230000003287 optical effect Effects 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 142
- 238000013016 damping Methods 0.000 description 17
- 108010066057 cabin-1 Proteins 0.000 description 11
- 239000013078 crystal Substances 0.000 description 7
- 229920000049 Carbon (fiber) Polymers 0.000 description 6
- 239000004917 carbon fiber Substances 0.000 description 6
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 6
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 6
- 239000000203 mixture Substances 0.000 description 5
- 239000007788 liquid Substances 0.000 description 4
- 230000003534 oscillatory effect Effects 0.000 description 4
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 3
- 239000000919 ceramic Substances 0.000 description 3
- 238000009792 diffusion process Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
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- 229910052697 platinum Inorganic materials 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 229910052710 silicon Inorganic materials 0.000 description 3
- 239000010703 silicon Substances 0.000 description 3
- 238000010257 thawing Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000002659 cell therapy Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
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- 230000036541 health Effects 0.000 description 2
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- 239000010980 sapphire Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000011357 CAR T-cell therapy Methods 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
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- 208000037887 cell injury Diseases 0.000 description 1
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- 230000003111 delayed effect Effects 0.000 description 1
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- 238000006073 displacement reaction Methods 0.000 description 1
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- 239000000446 fuel Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
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- 235000015110 jellies Nutrition 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
Abstract
The invention discloses a kind of apparatus for the anhydrous dry type recovery of cell, including recovery cabin, pedestal and collet formula evenly mixing device, recovery freight space is above pedestal, recovery cabin includes recovery cabin and the recovery hatchcover for being movably connected on recovery cabin, recovery hatchcover can be covered on recovery cabin, form closed recovery space, double function rewarming pallets are equipped in recovery cabin, double function rewarming heat lids are equipped in recovery hatchcover, the power source that collet formula rubs evenly mixing device is set in pedestal, is flexibly connected by powered support with double function rewarming pallets.The present invention is suitable for the anhydrous dry type recovery application of cell cryopreservation bag or cell cryopreservation tube.
Description
Technical field
The present invention relates to cell recovery fields, and in particular to a kind of apparatus for the anhydrous dry type recovery of cell.
Background technique
With the development of Life Science, cell therapy technology is maked rapid progress, main including stem cell therapy and thin with CAR-T
Born of the same parents are the immune cell therapy of representative.Cell involved in the preparation process of cell product freeze and defrosting of recovering.Cell recovery
When defrosting, optimum operation is that the cell cryopreservation bag or cell cryopreservation tube that are just taken out by liquid nitrogen container are rapidly heated to 37 DEG C, is allowed to
Most easily damaged -5 DEG C~0 DEG C of cell is run through, recrystallizes to avoid ice crystal to cell damage, leads to the dead of cell
It dies.
Cell cryopreservation container is common cell cryopreservation tube and two kinds of cell cryopreservation bag.Cell cryopreservation tube how small freeze capacity
Cell cryopreservation demand multiselect cell cryopreservation bag in 5ml, more than 5ml.Cell cryopreservation bag is more suitable for stem cell and is immunized thin
The commercial applications of born of the same parents, such as CAR-T cell therapy.Currently, water-bath recovery mode or dry can be used in the recovery of cell cryopreservation bag
Formula recovery mode, the former water-bath recovery mode need whole manual operation, can not carry out normalizing operation, and it is thin to be easy pollution
Born of the same parents gradually eliminate and use, and the current product of the latter's dry type recovery mode is limited, and recovery mode is single, and recovery efficiency is still not to the utmost
Such as people's will, also it is significantly improved and growth requirement.
In any heating device, thermal energy of the heat source in the form of three kinds of convection current, radiation and conduction passes to heating object
Body.It is thermally conductive to refer to when object each section is directly contacted without relative displacement or different objects by material molecule, atom and free electricity
The warm-up movement of the microcosmic particles such as son and the heat transfer phenomenon carried out.Convection current is by fluid motion, heat by a transmitting
The phenomenon that another place.Either thermally conductive or convection current, it is necessary to directly contact or rely on conventional substances by cold and hot object
Heat is transmitted for medium.But the principle of heat radiation is then entirely different, it be externally emit by body surface it is visible and can not
The ray seen transmits heat.The transmission speed of radiant heating is fast, and not by any medium, thus greatly reduces thermal energy biography
Loss during passing, to improve heat utilization rate.
In far infrared heating technology based on prominent radiant heating.When far infrared is irradiated to the object being heated, one
Ray is divided to be reflected back, a part is pierced over.When the far infrared wavelength of transmitting and the absorbing wavelength of heating object
When consistent, the object being heated largely absorbs far infrared so that interior of articles molecule and atom occur "Resonance" --- it generates
Strong vibration, rotation, and vibrate and rotation increases object temperature, to reach heating purpose.
Far infrared is a kind of light being invisible to the naked eye, it, which is different from general infrared ray (near infrared ray), only has human body
Thermotherapy effect；It is converted into Far-infrared spoke ray via thermal energy of the specific infra-red material expeditiously by arround；And 3~10 μm
The far infrared of wave-length coverage can be absorbed by hydrone and the intracorporal organic molecule of biology, therefore energy can be passed into
In vivo, induce generate a succession of pair of human health beneficial to physiological action.Using this mechanism, far infrared can be answered widely
With in health care, referred to as " far infrared therapy ".
The energy transmitting mode of carbon-crystal electric hot plate or carbon fiber electrothermal plate be based on far infrared radiation, carbon-crystal electric hot plate or
Carbon fiber electrothermal plate can give off the infrared energy that can heat substance after energization.The infrared wave of radiation a length of 2.3
~14 μm, the far infrared line spectrum of this section of wavelength is referred to as " light of life ", accounts for 80% or more of whole wavelength.It can be by moisture
Son, which absorbs, generates resonance friction fuel factor, realizes the effect for quickly improving heated material temperature.Especially can effectively it live
Change human tissue cell, promotes blood circulation, accelerate newly to be metabolized, enhance the health-care effects such as immunocompetence.
Currently, still not simultaneously using warm plate conduction heating and Far-infrared ray radiation heating while to carry out cell jelly
Deposit the anhydrous dry type recovery application of bag or cell cryopreservation tube.The present invention is therefore.
Summary of the invention
It is an object of the invention to provide a kind of apparatus for the anhydrous dry type recovery of cell, solve the above-mentioned prior art and ask
One or more in topic.
A kind of apparatus for the anhydrous dry type recovery of cell according to the present invention, including recovery cabin, pedestal and collet
Formula evenly mixing device, the recovery freight space is above the pedestal；
The recovery cabin includes recovery cabin and the recovery hatchcover for being movably connected on the recovery cabin, the recovery hatchcover
It can be covered on the recovery cabin, form closed recovery space, double function rewarming pallets are equipped in the recovery cabin, it is described multiple
Hatchcover of reviving is interior to be equipped with double function rewarming heat lids；
The collet formula evenly mixing device be set to the pedestal in, and with the intracorporal double function rewarming pallets in the recovery cabin
It is connected.
A kind of apparatus for the anhydrous dry type recovery of cell provided by the present invention, can be not only used for cell cryopreservation bag
Anhydrous dry type recovery, and can be used for the anhydrous dry type recovery of high-flux cell cryopreservation tube；Conduction heating, far infrared will be contacted simultaneously
Beta radiation heats two kinds of rewarming modes and combines, and the rewarming of cell cryopreservation bag or cell cryopreservation tube is more efficient.
In some embodiments, double function rewarming pallets are detachable and interchangeable, double function rewarming heat lids with it is described multiple
Buffer unit and pressure sensor are equipped between Soviet Union's hatchcover, double function rewarming heat lids and the recovery hatchcover pass through described
Buffer unit and pressure sensor are flexibly connected, and double function rewarming heat lids are detachable and interchangeable, are additionally provided in the recovery hatchcover
Exhaust fan.
In some embodiments, air is sucked from the ventilation opening of the base bottom, side wall and rear wall, along recovery cabin chamber
The gap in internal portion rises, and is discharged by the exhaust fan, and the pedestal is equipped with numerical control device, communication device and touch screen.
In some embodiments, double function rewarming pallets include tray frame and the first calandria, the tray frame
Frame is made of metal, and first calandria is set in the tray frame, and first calandria is while there is conduction to add
The electric hot plate of heat and two kinds of heating functions of Far-infrared ray radiation heating, first calandria and the tray frame are fixed to be connected
It connects, the tray frame is equipped with the first temperature control temperature sensor, and the first temperature control temperature number of sensors is 1~6, described
First calandria is equipped with through-hole, and the first rewarming monitoring temperature sensor is equipped in through-hole, and the first rewarming monitoring temperature passes
Sensor number is 1~6；
The operating temperature range of double function rewarming pallets is 10 DEG C~70 DEG C, is painted with cell on double function rewarming pallets
Freeze the placement position guide line of bag or cell cryopreservation tube.
In some embodiments, the temperature control temperature sensor and the rewarming monitoring temperature sensor include contact
Temperature sensor and non-contact temperature sensor, the contact type temperature sensor include thermistor (temperature) sensor, thermocouple
Sensor, thermocouple string sensor, digital sensor, platinum sensor etc., the non-contact temperature sensor includes thermoelectricity
Heap array non-contact temperature sensor, infrared temperature probe temperature sensor, thermal infrared imager temperature sensor etc..
In some embodiments, double function rewarming heat lids include hot cover frame and the second calandria, the hot lid frame
Frame is made of metal, and second calandria is located in the hot cover frame, and second calandria is while there is conduction to add
The electric hot plate of heat and two kinds of heating functions of Far-infrared ray radiation heating, second calandria and the fixed company of the hot cover frame
It connects, the hot cover frame is equipped with the second temperature control temperature sensor, and the second temperature control temperature number of sensors is 1~6, described
Second calandria is equipped with through-hole, and the second rewarming monitoring temperature sensor is equipped in through-hole, and the second rewarming monitoring temperature passes
Sensor number is 1~6, and the operating temperature range of double function rewarming heat lids is 10 DEG C~70 DEG C, double function rewarming heat lids
On be painted with the placement position guide line of cell cryopreservation bag or cell cryopreservation tube.
In some embodiments, the buffer unit be hydraulic bjuffer, spring buffer or damping buffer, it is described
Damping buffer includes that air class damping buffer, gear class damping buffer, rack gear class damping buffer, cylindric damping are slow
Rush device.
In some embodiments, the pressure sensor is piezoresistive pressure sensor, ceramic pressure sensor, diffusion
Silicon pressure sensor, sapphire pressure sensor, piezoelectric pressure indicator.
In some embodiments, the collet formula evenly mixing device includes power source and powered support, and the power source is logical
It crosses bolt and is fixedly connected on bottom in the pedestal, the power source includes servo motor and motor fixing frame, the servo electricity
The motor shaft end of machine is equipped with eccentric wheel, and the number of the servo motor is 2 or 4, and the number of the powered support is 4,
The number of the eccentric wheel is 4, and the dimensions of 4 eccentric wheels is identical, and when installation can adjust 4 bias respectively
The original eccentricity distance of vertical direction is taken turns, the eccentric wheel and double function rewarming pallets are passed through ball-and-socket type knot by the powered support
Structure is flexibly connected, and by the regulation of the numerical control device, the collet formula evenly mixing device can drive institute for the operation of the servo motor
It states double function rewarming pallets and realizes that collet formula rubs oscillation and mixes movement.
In some embodiments, the collet formula evenly mixing device can also be electronic fast hydraulic pressure mandril, electric hydaulic pushes away
The number of bar, electric pushrod etc., the electronic fast hydraulic pressure mandril or electric drive hydraulic thruster or electric pushrod etc. be it is a, respectively
It is flexibly connected with double function rewarming pallets, the electronic fast hydraulic pressure mandril or electric drive hydraulic thruster or electric pushrod etc.
Regulation of the operation by the numerical control device.
In some embodiments, the communication device can with internet or local area network by wired or wireless networking, into
Row data exchange, data storage and data query.
A kind of working method of the apparatus for the anhydrous dry type recovery of cell, wherein include the following steps:
(1) start instrument, set recovery required temperature, select resuscitative efforts mode, open pre- heat function, double function rewarming supports
Disk and double function rewarming heat lids start to preheat, and the temperature of double function rewarming pallets and double function rewarming heat lids starts to increase, and emit remote red
Outside line after preheating, cell cryopreservation bag or cell cryopreservation tube is placed on double function rewarming pallets, covers and fixes recovery cabin
Lid, under the action of buffer unit between double function rewarming heat lids and recovery hatchcover, cell cryopreservation bag or cell cryopreservation tube are double
Function rewarming pallet and double function rewarming heat lids clamp, and cell recovery starts, and double function rewarming pallets and double function rewarming heat are covered and frozen to cell
It deposits bag or cell cryopreservation tube while passing through two kinds of heat exchange pattern transmitting heats of conduction and radiation, collet formula evenly mixing device starts work
Make, under the regulation of numerical control device, the starting operating angle of 4 eccentric wheels is different, and the speed of service is different, drives double function rewarming supports
Disk, cell cryopreservation bag or cell cryopreservation tube, double function rewarming heat lids carry out collet formula together and rub oscillation mixing movement, mix movement
The strength applied can be detected by the pressure sensor between double function rewarming heat lids and recovery hatchcover, be transmitted to numerical control device,
Intelligence Feedback adjusts the operation of power source, prevents the application excessive damaging cells of strength from freezing bag or instrument；
(2) double function rewarming pallets and double function rewarming heat lids by temperature control temperature sensor monitor at any time double function rewarming pallets with
Double function rewarming heat cover the temperature of itself, and detection data is transferred to numerical control device, and numerical control device controls double function rewarming pallets and double function
The heating process of rewarming heat lid, intelligently maintains set temperature；
(3) the rewarming mesh of rewarming monitoring temperature sensor detection cell cryopreservation bag or cell cryopreservation tube Current Temperatures and setting
Whether mark temperature matches, and matches and stablizes the judgement time not higher than 5 seconds, if being lower than rewarming target temperature, opens and keep to heat
Rewarming state stops heating if being higher than rewarming target temperature immediately；
(4) when being in heating mode, double function heating pallets and double function rewarming heat lids are in heating state, disk body and Re Gaiwen
Degree increases, and emitting far-infrared, and heat is conducted and radiated two kinds of heat exchange patterns to cell cryopreservation bag or cell cryopreservation tube
It transmits heat and carries out rewarming；
(5) when rewarming monitoring temperature sensor detects that cell cryopreservation bag temperature is more than 0 DEG C~8 DEG C, it can determine whether that cell freezes
It deposits after the ice cube in bag or cell cryopreservation tube melts completely, then closes heating mode, double function rewarming pallets and double function rewarming heat lids
It stops working, while starting exhaust fan, air is sucked from the ventilation opening of the bottom of pedestal, side wall and rear wall, along recovery cavity
Internal gap rises, and is finally discharged from exhaust fan, takes away the heat of double function rewarming pallets and double function rewarming heat lids, and collet formula is mixed
Even device persistently carries out collet formula and rubs oscillation mixing movement；
(6) cell recovery of cell cryopreservation bag or cell cryopreservation tube work is completed, and indicator light flashing, loudspeaker sounding are passed through
Equal sound and light signals remind operator to take the cell cryopreservation bag or cell cryopreservation tube recovered away in time.
The present invention have the advantage that and the utility model has the advantages that
1, the present invention can be not only used for the anhydrous dry type recovery of cell cryopreservation bag, and can be used for the nothing of high-flux cell cryopreservation tube
The recovery of water dry type；
2, the present invention uses a kind of electric hot plate member simultaneously with two kinds of functions of flat heater and Far-infrared ray radiation heating
Device, while contact two kinds of conduction heating, Far-infrared ray radiation heating rewarming modes being combined, make cell cryopreservation bag or
The rewarming of cell cryopreservation tube is more efficient；
3, double function rewarming pallets are placed in recovery cabin, can be carried out the oscillation of collet formula and be mixed movement, built-in mixing work
Mode compared to the mixing operating mode of external is not necessarily to that seal operation cabin, double function rewarming supports are arranged on double function rewarming pallets
Consolidation body weight is light, and structure is simpler, is easy to technology realization；
4, the number of the servo motor of the collet formula evenly mixing device of double function rewarming pallets of four-point supporting type is 4, utilizes 4
4 eccentric wheels are flexibly connected with double function rewarming pallets by 4 supporting points by a connecting rod, and the operation of servo motor receives numerical control
The regulation of device, by changing the revolving speed of servo motor and the starting angle of operation of eccentric wheel, double function rewarmings of four-point supporting type
Pallet may be implemented the oscillation of complicated and diversified collet formula and mix movement, and mixed effect is significantly stronger than only 1 servo motor
Two o'clock support type seesaw type mixed effect；
5, the buffer unit in collet formula evenly mixing device cooperation recovery hatchcover, can make cell cryopreservation bag or cell cryopreservation tube
It is clamped by double function rewarming pallets and double function rewarming heat lids, is conducive to heat transfer, collet formula evenly mixing device can apply cell cryopreservation bag
Add extruding to act, is very beneficial for the flowing mixing of cell cryopreservation bag liquid, it can be high by the relative temperature heated by periphery
Liquid close on the low liquid of the relative temperature of ice cube with inside and mix in time, on the one hand cell is protected to be not affected by high temperatures and lose
It is living, on the other hand convenient for uniformly taking around ice cube heat to rapidly, realize that quick-thawing melts；
6, it is additionally provided with pressure sensor in addition to buffer unit between recovery hatchcover and double function rewarming heat lids, collet formula mixes
The strength that the power source of device is applied can be detected by the pressure sensor between double function rewarming heat lids and recovery hatchcover, be transmitted
To numerical control device, Intelligence Feedback adjusts the operation of power source, prevents from applying the excessive damage for leading to cell cryopreservation bag or instrument of strength
Wound；
7, exhaust fan is additionally provided in recovery hatchcover, air is sucked from the ventilation opening of base bottom, side wall and rear wall, along recovery
Gap inside cabin rises, and is finally discharged from exhaust fan, takes away the heat of double function rewarming pallets and double function rewarming heat lids, is conducive to
Double function rewarming pallets and double hot lid amount of residual heat of function rewarming are reduced after rewarming to the shadow of cell cryopreservation bag or cell cryopreservation tube
It rings.
Detailed description of the invention
Fig. 1 is that a kind of structure of apparatus for the anhydrous dry type recovery of cell of one embodiment of the present invention is shown
It is intended to；
Fig. 2 is that a kind of double function of apparatus for the anhydrous dry type recovery of cell of one embodiment of the present invention are multiple
Warm pallet, double function rewarming heat lids, buffer unit and collet formula evenly mixing device axonometric drawing；
Fig. 3 is a kind of vertical view of apparatus for the anhydrous dry type recovery of cell of one embodiment of the present invention
Figure；
Fig. 4 is that a kind of double function of apparatus for the anhydrous dry type recovery of cell of one embodiment of the present invention are multiple
The right view of warm pallet, double function rewarming heat lids and buffer unit；
Fig. 5 is that a kind of double function of apparatus for the anhydrous dry type recovery of cell of one embodiment of the present invention are multiple
Warm pallet, double function rewarming heat lids, buffer unit and collet formula evenly mixing device axis survey bottom view.
Specific embodiment
With reference to the accompanying drawings of the specification, the present invention is described in more detail.
Embodiment 1:
As shown in fig. 1, a kind of apparatus for the anhydrous dry type recovery of cell, including recovery cabin 1, pedestal 2 and bottom
Support formula evenly mixing device 3, recovery cabin 1 are installed on 2 top of pedestal, and recovery cabin 1 includes recovery cabin 11 and is movably connected on recovery cabin
The recovery hatchcover 12 of body 11, recovery hatchcover 12 can be covered on the recovery cabin 11, form closed recovery space, recovery cabin
It is equipped with double function rewarming pallets 111 in 11, the hot lid 121 of double function rewarmings, double function rewarming pallets 111 and double function are equipped in recovery hatchcover 12
Conjunction can be covered between the hot lid 121 of rewarming, cell cryopreservation bag or cell cryopreservation tube can be placed on double function rewarming pallets 111 and double function rewarming heat
Recovery operation is carried out between lid 121.
Collet formula evenly mixing device 3 is that collet formula rubs evenly mixing device, and collet formula rubs evenly mixing device and is installed in pedestal 2,
And be connected with double function rewarming pallets 111 in recovery cabin 11, recovery cabin 1 is fixed in the present embodiment, is pressed by collet formula
Rubbing evenly mixing device drives double 111 progress collet formulas of function rewarming pallets to rub oscillation mixing movement.
A kind of apparatus for the anhydrous dry type recovery of cell provided by the present invention, can be not only used for cell cryopreservation bag
Anhydrous dry type recovery, and can be used for the anhydrous dry type recovery of high-flux cell cryopreservation tube；Conduction heating, far infrared will be contacted simultaneously
Beta radiation heats two kinds of rewarming modes and combines, and the rewarming of cell cryopreservation bag or cell cryopreservation tube is more efficient.
Among the above, as shown in Fig. 2~4, double function rewarming pallets 111 are detachable and interchangeable, double hot lids 121 of function rewarming and recovery
Buffer unit 122 and pressure sensor 124 are installed, double hot lids 121 of function rewarming pass through slow with recovery hatchcover 12 between hatchcover 12
Flushing device 122 and pressure sensor 124 are flexibly connected, and pressure sensor 124 is installed among buffer unit 122 or side, are delayed
Flushing device 122 is spring in the present embodiment, and one end, which is bolted, is installed on hot 121 four corners of lid of double function rewarmings, another
End, which is bolted, to be installed on recovery hatchcover 12, and double hot lids 121 of function rewarming are also detachable and interchangeable, also set in recovery hatchcover 12
There is exhaust fan 123.Double function rewarming pallets 111 simultaneously will contact conduction heating and far-infrared radiation with the hot lid 121 of double function rewarmings
It heats two kinds of rewarming modes to combine, rewarming is more efficient.
Air is sucked from the ventilation opening of 2 bottom of pedestal, side wall and rear wall, is risen along the gap of 1 inside cavity of recovery cabin, by
Exhaust fan 123 is discharged, and pedestal 2 is equipped with numerical control device 21, communication device 22 and touch screen 23.
Double function rewarming pallets 111 include tray frame 1111 and the first calandria 1112, and the tray frame 1111 is by gold
Category is made, and the first calandria 1112 is installed in tray frame 1111, the first calandria 1112 be simultaneously have conduction heating with
The electric hot plate of two kinds of heating functions of Far-infrared ray radiation heating, electric hot plate include carbon-crystal electric hot plate, carbon fiber electrothermal plate etc., and first
Calandria 1112 is bolted to connection with tray frame 1111, and tray frame 1111 is built-in with the first temperature control temperature sensing
Device, can the first calandria 1112 described in at-once monitor temperature, the first temperature control temperature number of sensors is 1~6, the first heating
Through-hole is offered on body 1112, is equipped with the first rewarming monitoring temperature sensor 1114 in through-hole, it can at-once monitor cell cryopreservation bag
Temperature, 1114 number of the first rewarming monitoring temperature sensor be 1~6, in the present embodiment quantity be 2.
The operating temperature range of double function rewarming pallets 111 is 10 DEG C~70 DEG C, can be set by touch screen 23, double function
The placement position guide line of cell cryopreservation bag or cell cryopreservation tube is painted on rewarming pallet 111, double function rewarming pallets 111 can be flat
Chassis is suitble to the dry type recovery of cell cryopreservation bag.
Double function rewarming pallets 111 can also offer multiple cryopreservation tube vallecular cavities, and cryopreservation tube vallecular cavity is in long rectangle, and cross section is in half
Round shape, is suitble to the dry type recovery of high-flux cell cryopreservation tube, and double function rewarming pallets 111 are filled with the numerical control device 21 by connector
Set electrical connection.
Temperature control temperature sensor 1113 and rewarming monitoring temperature sensor 1114 include contact type temperature sensor and non-connect
Touch temperature sensor, contact type temperature sensor include thermistor (temperature) sensor, thermocouple sensor, thermocouple string sensing
Device, digital sensor, platinum sensor etc., non-contact temperature sensor include thermopile array non-contact temperature sensing
Device, infrared temperature probe temperature sensor, thermal infrared imager temperature sensor etc..
Double hot lids 121 of function rewarming include hot cover frame 1211 and the second calandria 1212, and hot cover frame 1211 is by made of metal
At the second calandria 1212 is located in hot cover frame 1211, and the second calandria 1212 is while having conduction heating and far infrared
Beta radiation heats the electric hot plate of two kinds of heating functions, and electric hot plate includes carbon-crystal electric hot plate, carbon fiber electrothermal plate etc., the second calandria
1212 are fixedly connected with hot cover frame 1211, and hot cover frame 1211 is equipped with the second temperature control temperature sensor 1213, can at-once monitor
The temperature of second calandria 1212,1213 number of the second temperature control temperature sensor are 1~6, and the second calandria 1212 is equipped with
Through-hole is equipped with the second rewarming monitoring temperature sensor 1214 in through-hole, can at-once monitor cell cryopreservation bag temperature, the second rewarming
1214 number of monitoring temperature sensor is 1~6, and the operating temperature range of double hot lids 121 of function rewarming is 10 DEG C~70 DEG C, can be led to
It crosses touch screen 23 to be set, the placement position guidance of cell cryopreservation bag or cell cryopreservation tube is painted on double hot lids 121 of function rewarming
Line.
Double hot lids 121 of function rewarming can be tabular, be suitble to the dry type recovery of cell cryopreservation bag, double hot lids 121 of function rewarming are also
Multiple cryopreservation tube vallecular cavities can be offered, cryopreservation tube vallecular cavity is in long rectangle, and cross section is in semicircle shape, is suitble to high-flux cell cryopreservation tube
Dry type recovery, the cryopreservation tube vallecular cavity of double hot lids 121 of function rewarming and the cryopreservation tube vallecular cavity of pair function rewarming pallets 111 be correspondingly arranged,
Double hot lids 121 of function rewarming are electrically connected with numerical control device 21 by piecing devices.
Buffer unit 122 is hydraulic bjuffer, spring buffer or damping buffer, and damping buffer includes the resistance of air class
Buddhist nun's buffer, gear class damping buffer, rack gear class damping buffer, cylindric damping buffer.
Pressure sensor 124 is piezoresistive pressure sensor, ceramic pressure sensor, diffusion silicon pressure sensor, Lan Bao
Stone pressure sensor, piezoelectric pressure indicator.
As shown in Figure 5, it includes power source 31 and powered support 32 that collet formula, which rubs evenly mixing device, and power source 31 is fixed to be connected
It is connected to bottom in pedestal 2, power source 31 includes servo motor 311 and motor fixing frame 312, the motor shaft end of servo motor 311
Equipped with eccentric wheel 313, the number of servo motor 311 is 2 or 4, and the number of powered support 32 is 4, the number of eccentric wheel 313
Mesh is 4, and the dimensions of 4 eccentric wheels 313 is identical, and when installation can adjust the initial of 4 313 vertical directions of eccentric wheel respectively
Eccentric wheel 313 is connect with double function rewarming pallets 111 by ball-and-socket type structure movement by eccentricity, powered support 32, servo motor
By the regulation of numerical control device 21, collet formula, which rubs evenly mixing device 3, can drive double function rewarming pallets 111 to realize collet for 311 operation
Formula rubs oscillation and mixes movement.
Among the above, collet formula, which rubs evenly mixing device, can also be electronic fast hydraulic pressure mandril, electric drive hydraulic thruster, electric pushrod
Number Deng, electronic fast hydraulic pressure mandril or electric drive hydraulic thruster or electric pushrod etc. is 4, respectively with double function rewarming pallets
111 are flexibly connected, and the operation of electronic fast hydraulic pressure mandril or electric drive hydraulic thruster or electric pushrod etc. is by the numerical control device 21
Regulation.
Communication device 22 can carry out data exchange, data storage with internet or local area network by wired or wireless networking
And data query.
A kind of apparatus for the anhydrous dry type recovery of cell of embodiment 1, recovery cabin and pedestal, which are that one is fixed, to be connected
It connects, double function rewarming pallets are placed in recovery cabin, and collet formula, which rubs evenly mixing device, can drive double function rewarming pallets to carry out collet formula
It rubs oscillation and mixes movement, double function rewarming pallets move in recovery cabin, are a kind of built-in mixing operating modes.
The working method of a kind of apparatus for the anhydrous dry type recovery of cell of embodiment 1, wherein including walking as follows
It is rapid:
(1) start instrument, set recovery required temperature, select resuscitative efforts mode, open pre- heat function, double function rewarming supports
Disk and double function rewarming heat lids start to preheat, and the temperature of double function rewarming pallets and double function rewarming heat lids starts to increase, and emit remote red
Outside line after preheating, cell cryopreservation bag or cell cryopreservation tube is placed on double function rewarming pallets, covers and fixes recovery cabin
Lid, under the action of buffer unit between double function rewarming heat lids and recovery hatchcover, cell cryopreservation bag or cell cryopreservation tube are double
Function rewarming pallet and double function rewarming heat lids clamp, and cell recovery starts, and double function rewarming pallets and double function rewarming heat are covered and frozen to cell
It deposits bag or cell cryopreservation tube while passing through two kinds of heat exchange pattern transmitting heats of conduction and radiation, collet formula rubs evenly mixing device and opens
Beginning work, under the regulation of numerical control device, the starting operating angle of 4 eccentric wheels is different, and the speed of service is different, drives double function multiple
Warm pallet, cell cryopreservation bag or cell cryopreservation tube, double function rewarming heat lids carry out collet formula together and rub oscillation mixing movement, mix
Moving applied strength can be detected by the pressure sensor between double function rewarming heat lids and recovery hatchcover, be transmitted to numerical control dress
It sets, Intelligence Feedback adjusts the operation of power source, prevents the application excessive damaging cells of strength from freezing bag or instrument；
(2) double function rewarming pallets and double function rewarming heat lids by temperature control temperature sensor monitor at any time double function rewarming pallets with
Double function rewarming heat cover the temperature of itself, and detection data is transferred to numerical control device, and numerical control device controls double function rewarming pallets and double function
The heating process of rewarming heat lid, intelligently maintains set temperature；
(3) the rewarming mesh of rewarming monitoring temperature sensor detection cell cryopreservation bag or cell cryopreservation tube Current Temperatures and setting
Whether mark temperature matches, and matches and stablizes the judgement time not higher than 5 seconds, if being lower than rewarming target temperature, opens and keep to heat
Rewarming state stops heating if being higher than rewarming target temperature immediately；
(4) when being in heating mode, double function heating pallets and double function rewarming heat lids are in heating state, disk body and Re Gaiwen
Degree increases, and emitting far-infrared, and heat is conducted and radiated two kinds of heat exchange patterns to cell cryopreservation bag or cell cryopreservation tube
It transmits heat and carries out rewarming；
(5) when rewarming monitoring temperature sensor detects that cell cryopreservation bag temperature is more than 0 DEG C~8 DEG C, it can determine whether that cell freezes
It deposits after the ice cube in bag or cell cryopreservation tube melts completely, then closes heating mode, double function rewarming pallets and double function rewarming heat lids
It stops working, while starting exhaust fan, air is sucked from the ventilation opening of the bottom of pedestal, side wall and rear wall, along recovery cabin
The gap in portion rises, and is finally discharged from exhaust fan, takes away the heat of double function rewarming pallets and double function rewarming heat lids, and collet formula rubs
Evenly mixing device persistently carries out collet formula and rubs oscillation mixing movement；
(6) cell recovery of cell cryopreservation bag or cell cryopreservation tube work is completed, and indicator light flashing, loudspeaker sounding are passed through
Equal sound and light signals remind operator to take the cell cryopreservation bag or cell cryopreservation tube recovered away in time.
Embodiment 2:
A kind of apparatus for the anhydrous dry type recovery of cell, including recovery cabin 1, pedestal 2 and collet formula evenly mixing device
3, collet formula evenly mixing device 3 is collet formula three-dimensional evenly mixing device, 2 split-type design of recovery cabin 1 and pedestal, the mixing of collet formula three-dimensional
Device is installed in pedestal 2, and recovery cabin 1 and pedestal 2 are flexibly connected by the powered support 32 of collet formula three-dimensional evenly mixing device, bottom
Support formula three-dimensional evenly mixing device supports entire recovery cabin 1 by 4 powered supports 32.
Recovery cabin 1 includes recovery cabin 11 and recovery hatchcover 12, and recovery cabin 11 is flexibly connected with recovery hatchcover 12, is recovered
Hatchcover 12 can be covered on recovery cabin 11, form closed recovery space, and double function rewarming pallets 111 are equipped in recovery cabin 11,
Double function rewarming pallets 111 are detachable and interchangeable, are equipped with the hot lid 121 of double function rewarmings in recovery hatchcover 12, double hot lids 121 of function rewarming with it is multiple
Hatchcover 12 of reviving is flexibly connected, and is equipped with buffer unit 122 and pressure experience device between the hot lid 121 of double function rewarmings and recovery hatchcover 12
124, double hot lids 121 of function rewarming are detachable and interchangeable.
Numerical control device, communication device and touch screen are additionally provided in pedestal 2.
Double function rewarming pallets 111 include tray frame 1111 and the first calandria 1112, and tray frame 1111 is by made of metal
At the first calandria 1112 is located in tray frame 1111, and the first calandria 1112 is while having conduction heating and far infrared
Beta radiation heats the electric hot plate of two kinds of heating functions, and electric hot plate includes carbon-crystal electric hot plate, carbon fiber electrothermal plate etc., the first calandria
1112 are fixedly connected with tray frame 1111, and tray frame 1111 is built-in with temperature control temperature sensor, can at-once monitor first plus
The temperature of hot body 1112, temperature control temperature number of sensors are 1~6, and preferably 2,1112 centre bit of the first calandria installs
Have a through-hole, rewarming monitoring temperature sensor 1114 be equipped in through-hole, can at-once monitor cell cryopreservation bag temperature, rewarming monitoring temperature
Spending 1114 number of sensor is 1~6, and preferably 2, the operating temperature range of double function rewarming pallets 111 is 10 DEG C~70 DEG C,
It can be set by touch screen, be painted with cell cryopreservation bag on double function rewarming pallets 111 or the placement position of cell cryopreservation tube refers to
Conducting wire.
Double function rewarming pallets 111 can be flat chassis, be suitble to the dry type recovery of cell cryopreservation bag, double function rewarming pallets 111 are also
Multiple cryopreservation tube vallecular cavities can be offered, cryopreservation tube vallecular cavity is in long rectangle, and cross section is in semicircle shape, is suitble to high-flux cell cryopreservation tube
Dry type recovery, double function rewarming pallets 111 are electrically connected with recovery cabin 11 by piecing devices.
Temperature control temperature sensor and rewarming monitoring temperature sensor include contact type temperature sensor and non-contact temperature
Sensor, contact type temperature sensor include thermistor (temperature) sensor, thermocouple sensor, thermocouple string sensor, number biography
Sensor and platinum sensor etc., non-contact temperature sensor include thermopile array non-contact temperature sensor, infrared survey
Warm probe temperature sensor, thermal infrared imager temperature sensor etc..
Double hot lids 112 of function rewarming include hot cover frame 1121 and the second calandria 1122, and hot cover frame 1121 is by made of metal
At the second calandria 1122 is located in hot cover frame 1121, and the second calandria 1122 is while having conduction heating and far infrared
Beta radiation heats the electric hot plate of two kinds of heating functions, and electric hot plate includes carbon-crystal electric hot plate, carbon fiber electrothermal plate etc., the second calandria
1122 are fixedly connected with hot cover frame 1121, and hot cover frame 1121 is equipped with temperature control temperature sensor 1123, can at-once monitor second
The temperature of calandria 1122,1123 number of temperature control temperature sensor be 1~6, preferably 2, the work of double hot lids 112 of function rewarming
Make temperature range be 10 DEG C~70 DEG C, can be set by touch screen, be painted on double hot lids 112 of function rewarming cell cryopreservation bag or
The placement position guide line of cell cryopreservation tube.
Double hot lids 112 of function rewarming can be tabular, be suitble to the dry type recovery of cell cryopreservation bag, double hot lids 112 of function rewarming are also
Multiple cryopreservation tube vallecular cavities can be offered, cryopreservation tube vallecular cavity is in long rectangle, and cross section is in semicircle shape, is suitble to high-flux cell cryopreservation tube
Dry type recovery, the cryopreservation tube vallecular cavity of the cryopreservation tube vallecular cavity of double hot lids 112 of function rewarming and double function rewarming pallets 111 corresponds,
Double hot lids 112 of function rewarming are electrically connected with recovery cabin 11 by piecing devices.
Buffer unit 122 includes hydraulic bjuffer, spring buffer, damping buffer, and damping buffer includes air class
Damping buffer, gear class damping buffer, rack gear class damping buffer, cylindric damping buffer.
Pressure sensor is piezoresistive pressure sensor, ceramic pressure sensor, diffusion silicon pressure sensor, sapphire pressure
Force snesor, piezoelectric pressure indicator.
Collet formula three-dimensional evenly mixing device includes power source 31 and powered support 32, and power source 31 is set in pedestal 2, fixed to connect
It is connected to bottom in pedestal 2, power source 31 includes servo motor 311, motor fixing frame 312 and eccentric wheel 313, servo motor 311
One or both ends be equipped with eccentric wheel 313, the number of servo motor 311 is 2 or 4, and the number of powered support 32 is 4,
The number of eccentric wheel 313 is 4, and powered support 32 connects eccentric wheel 313 and recovery cabin 1 by 4 ball-and-socket type tie point activities
Connect, the operation of servo motor 311 by numerical control device regulation, under the regulation of numerical control device, the starting work of 4 eccentric wheels 313
Make angle difference, the speed of service is different, and collet formula three-dimensional evenly mixing device can drive recovery cabin 1 integrally to realize that complicated three-dimensional is jolted
Oscillatory type mixes movement.
Collet formula three-dimensional evenly mixing device can also be electronic fast hydraulic pressure mandril, electric drive hydraulic thruster, electric pushrod etc., electronic
The number of fast hydraulic pressure mandril, electric drive hydraulic thruster, electric pushrod etc. is 4, passes through 4 tie point activities with recovery cabin respectively
Connection, regulation of the operation of electronic fast hydraulic pressure mandril, electric drive hydraulic thruster, electric pushrod etc. by numerical control device, the speed of service
Different with height, collet formula three-dimensional evenly mixing device can drive recovery cabin integrally to realize that complicated three-dimensional is jolted oscillatory type mixing
Movement.
Communication device can with internet or local area network by wired or wireless networking, carry out data exchange, data storage and
Data query.
A kind of apparatus for the anhydrous dry type recovery of cell of embodiment 2, recovery cabin and pedestal are split type settings
, collet formula three-dimensional evenly mixing device main body is set in pedestal, and recovery cabin and pedestal pass through the power of collet formula three-dimensional evenly mixing device
Bracket is flexibly connected, and collet formula three-dimensional evenly mixing device can drive recovery cabin integrally to carry out the complicated three-dimensional oscillatory type that jolts and mix fortune
Double function rewarming tray motions dynamic, that the movement in recovery cabin drives it internal, are a kind of mixing operating modes of external.
The working method of a kind of apparatus for the anhydrous dry type recovery of cell of embodiment 2, wherein including walking as follows
It is rapid:
(1) start instrument, select recovery mode, open pre- heat function, double function rewarming pallets and double function rewarming heat lids start
The temperature of preheating, double function rewarming pallets and double function rewarming heat lids starts to increase, and emitting far-infrared, will be thin after preheating
Born of the same parents freeze bag or cell cryopreservation tube is placed on double function rewarming pallets, cover and fix recovery hatchcover, cell recovery starts, double function
Rewarming pallet and double function rewarming heat are covered to cell cryopreservation bag or cell cryopreservation tube while passing through conduction and radiation two ways transmitting
Heat, collet formula three-dimensional evenly mixing device are started to work, and are driven entire recovery cabin to carry out the complicated three-dimensional oscillatory type that jolts and are mixed fortune
It is dynamic；
(2) double function rewarming pallets and double function rewarming heat lids by temperature control temperature sensor monitor at any time double function rewarming pallets with
Double function rewarming heat cover the temperature of itself, and detection data is transferred to numerical control device, and numerical control device controls double function rewarming pallets and double function
The heating process of rewarming heat lid, intelligently maintains set temperature；
(3) rewarming monitoring temperature sensor at-once monitor cell cryopreservation bag or cell cryopreservation tube Current Temperatures are answered with what is set
Whether warm target temperature matches, and matches and stablizes the judgement time not higher than 5 seconds, if being lower than rewarming target temperature, opens and keeps
Rewarming mode is heated, if being higher than rewarming target temperature, stops heating immediately；
(4) when being in heating mode, double function heating pallets and double function rewarming heat lids are in heating state, disk body and Re Gaiwen
Degree increases, and emitting far-infrared, and heat is conducted and radiated two ways to cell cryopreservation bag or cell cryopreservation tube transmitting heat
Amount carries out rewarming；
(5) when rewarming monitoring temperature sensor detects that cell cryopreservation bag temperature is more than 0 DEG C~8 DEG C, it can determine whether that cell freezes
It deposits after the ice cube in bag or cell cryopreservation tube melts completely, then closes heating mode, double function rewarming pallets and double function rewarming heat lids
It stops working, the cell recovery of cell cryopreservation bag or cell cryopreservation tube, which works, to be completed, and recovery hatchcover automatically opens, and passes through instruction
The sound and light signals such as lamp flashing, loudspeaker sounding remind operator to take the cell cryopreservation bag or cell cryopreservation tube recovered away in time.
The above is only preferred embodiment of the invention, it is noted that those skilled in the art, not
Under the premise of being detached from the invention design, some similar deformation and improvement can be made, these are also considered as invention protection
Within.
Claims (9)
1. a kind of apparatus for the anhydrous dry type recovery of cell, it is characterised in that: including recovery cabin (1), pedestal (2) and bottom
Support formula evenly mixing device (3), the recovery cabin (1) are located above the pedestal (2), and the recovery cabin (1) includes recovery cabin
(11) and it is movably connected on the recovery hatchcover (12) of the recovery cabin (11), the recovery hatchcover (12) can be covered on described multiple
It revives cabin (11), forms closed recovery space, double function rewarming pallets (111) are equipped in the recovery cabin (11), it is described multiple
Reviving, hatchcover (12) are interior to be equipped with double function rewarming heat lids (121)；The collet formula evenly mixing device (3) is set in the pedestal (2), and with
Double function rewarming pallets (111) in the recovery cabin (11) are connected.
2. a kind of apparatus for the anhydrous dry type recovery of cell according to claim 1, it is characterised in that: described double
Function rewarming pallet (111) is detachable and interchangeable, and buffering is equipped between double function rewarming heat lids (121) and the recovery hatchcover (12)
Device (122) and pressure sensor (124), double function rewarming heat lids (121) and the recovery hatchcover (12) pass through described slow
Flushing device (122) and pressure sensor (124) are flexibly connected, and double function rewarming heat lids (121) are detachable and interchangeable, the recovery
Exhaust fan (123) are additionally provided in hatchcover (12).
3. a kind of apparatus for cell anhydrous dry type recovery according to claim 2, it is characterised in that: air from
The ventilation opening of pedestal (2) bottom, side wall and rear wall sucks, and rises along the gap of recovery cabin (1) inside cavity, by the row
Fan (123) discharge, the pedestal (2) are equipped with numerical control device (21), communication device (22) and touch screen (23).
4. a kind of apparatus for the anhydrous dry type recovery of cell according to claim 2, it is characterised in that: described double
Function rewarming pallet (111) includes tray frame (1111) and the first calandria (1112), and the tray frame (1111) is by metal
It is made, first calandria (1112) is set in the tray frame (1111), and first calandria (1112) is simultaneously
Electric hot plate with two kinds of heating functions of conduction heating and Far-infrared ray radiation heating, first calandria (1112) with it is described
Tray frame (1111) is fixedly connected, and the tray frame (1111) is equipped with the first temperature control temperature sensor (1113), and described the
One temperature control temperature sensor (1113) number is 1~6, and first calandria (1112) is equipped with through-hole, is equipped in through-hole
First rewarming monitoring temperature sensor (1114), can at-once monitor cell cryopreservation bag temperature, the first rewarming monitoring temperature
Sensor (1114) number is 1~6；
The operating temperature range of double function rewarming pallets (111) is 10 DEG C~70 DEG C, is drawn on double function rewarming pallets (111)
There is the placement position guide line of cell cryopreservation bag or cell cryopreservation tube.
5. a kind of apparatus for the anhydrous dry type recovery of cell according to claim 2, it is characterised in that: described double
Function rewarming heat lid (121) includes hot cover frame (1211) and the second calandria (1212), and the hot cover frame (1211) is by metal
It is made, second calandria (1212) is located in the hot cover frame (1211), and second calandria (1212) is simultaneously
Electric hot plate with two kinds of heating functions of conduction heating and Far-infrared ray radiation heating, second calandria (1212) with it is described
Hot cover frame (1211) is fixedly connected, and the hot cover frame (1211) is equipped with the second temperature control temperature sensor (1213), and described the
Two temperature control temperature sensor (1213) numbers are 1~6, and second calandria (1212) is equipped with through-hole, are equipped in through-hole
Second rewarming monitoring temperature sensor (1214), the second rewarming monitoring temperature sensor (1214) number are 1~6, institute
The operating temperature range for stating double function rewarming heat lids (121) is 10 DEG C~70 DEG C, is painted with cell on double function rewarming heat lids (121)
Freeze the placement position guide line of bag or cell cryopreservation tube.
6. a kind of apparatus for the anhydrous dry type recovery of cell according to claim 2, it is characterised in that: the bottom
Support formula evenly mixing device (3) includes power source (31) and powered support (32), and the power source (31) is fixedly connected on the pedestal
(2) interior bottom, the power source (31) include servo motor (311) and motor fixing frame (312), the servo motor (311)
Motor shaft end be equipped with eccentric wheel (313), the number of the servo motor (311) is 2 or 4, the powered support (32)
Number be 4, the number of the eccentric wheel (313) is 4, and the dimensions of 4 eccentric wheels (313) is identical, installation
When can adjust the original eccentricity distances of 4 eccentric wheel (313) vertical directions respectively, the powered support (32) is by the bias
Wheel (313) is flexibly connected with double function rewarming pallets (111), and the operation of the servo motor (311) is by the numerical control device
(21) regulation, the collet formula evenly mixing device (3) can drive double function rewarming pallets (111) to realize that collet formula rubs oscillation
Mix movement.
7. a kind of apparatus for the anhydrous dry type recovery of cell according to claim 2, it is characterised in that: the bottom
Support formula evenly mixing device (3) can also be electronic fast hydraulic pressure mandril, electric drive hydraulic thruster or electric pushrod, the electronic fast hydraulic pressure
The number of mandril or electric drive hydraulic thruster or electric pushrod etc. is 4, respectively even with double function rewarming pallets (111) activities
It connects, the operation of the electronic fast hydraulic pressure mandril or electric drive hydraulic thruster or electric pushrod etc. is by the numerical control device (21)
Regulation.
8. a kind of apparatus for the anhydrous dry type recovery of cell according to claim 1, it is characterised in that: described logical
T unit (22) can with internet or local area network by wired or wireless networking, carry out data exchange, data storage sum number it is investigated that
It askes.
9. based on a kind of working method of apparatus for the anhydrous dry type recovery of cell described in claim 1~8,
In, include the following steps:
(1) start instrument, set recovery required temperature, select resuscitative efforts mode, open pre- heat function, double function rewarming pallets and
Double function rewarming heat lids start to preheat, and the temperature of double function rewarming pallets and double function rewarming heat lids starts to increase, and emitting far-infrared,
After preheating, cell cryopreservation bag or cell cryopreservation tube are placed on double function rewarming pallets, recovery hatchcover is covered and fix,
Under the action of buffer unit between double function rewarming heat lids and recovery hatchcover, cell cryopreservation bag or cell cryopreservation tube are by double function rewarmings
Pallet and double function rewarming heat lids clamp, and cell recovery starts, double function rewarming pallets and double function rewarming heat cover to cell cryopreservation bag or
Cell cryopreservation tube passes through two kinds of heat exchange pattern transmitting heats of conduction and radiation simultaneously, and collet formula evenly mixing device is started to work,
Under the regulation of numerical control device, the starting operating angle of 4 eccentric wheels is different, and the speed of service is different, drives double function rewarming pallets, thin
Born of the same parents freeze bag or cell cryopreservation tube, double function rewarming heat lids carry out the oscillation of collet formula together and mix movement, mix what movement was applied
Strength can be detected by the pressure sensor between double function rewarming heat lids and recovery hatchcover, be transmitted to numerical control device, Intelligence Feedback
The operation for adjusting power source prevents the application excessive damaging cells of strength from freezing bag or instrument；
(2) double function rewarming pallets and double function rewarming heat lids monitor double function rewarming pallets and pair function by temperature control temperature sensor at any time
Rewarming heat covers the temperature of itself, and detection data is transferred to numerical control device, and numerical control device controls double function rewarming pallets and double function rewarmings
The heating process of heat lid, intelligently maintains set temperature；
(3) the rewarming target temperature of rewarming monitoring temperature sensor detection cell cryopreservation bag or cell cryopreservation tube Current Temperatures and setting
Whether degree matches, and matches and stablizes the judgement time not higher than 5 seconds, if being lower than rewarming target temperature, opens and keep to heat rewarming
State stops heating if being higher than rewarming target temperature immediately；
(4) when being in heating mode, double function heating pallets and double function rewarming heat lids are in heating state, disk body and Re Gai temperature liter
Height, and it is emitting far-infrared, and heat is conducted and is radiated two kinds of heat exchange patterns and transmitted to cell cryopreservation bag or cell cryopreservation tube
Heat carries out rewarming；
(5) when rewarming monitoring temperature sensor detects that cell cryopreservation bag temperature is more than 0 DEG C~8 DEG C, it can determine whether cell cryopreservation bag
Or after the ice cube in cell cryopreservation tube melts completely, then heating mode, double function rewarming pallets and the hot Gai Junting of double function rewarmings are closed
It only works, while starting exhaust fan, air is sucked from the ventilation opening of the bottom of pedestal, side wall and rear wall, inside recovery cabin
Gap rises, and is finally discharged from exhaust fan, takes away the heat of double function rewarming pallets and double function rewarming heat lids, collet formula evenly mixing device
It persistently carries out the oscillation of collet formula and mixes movement；
(6) cell recovery of cell cryopreservation bag or cell cryopreservation tube work is completed, and the sound such as indicator light flashing, loudspeaker sounding are passed through
Optical signal reminds operator to take the cell cryopreservation bag or cell cryopreservation tube recovered away in time.
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| CN201910564380.4A CN110172400A (en) | 2019-06-26 | 2019-06-26 | A kind of apparatus for the anhydrous dry type recovery of cell |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910564380.4A CN110172400A (en) | 2019-06-26 | 2019-06-26 | A kind of apparatus for the anhydrous dry type recovery of cell |
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| CN110172400A true CN110172400A (en) | 2019-08-27 |
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Cited By (1)
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| CN109059418A (en) * | 2018-09-15 | 2018-12-21 | 乔燕春 | A kind of biological products freeze and recovery device and freeze and method for resuscitation |
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