CN206553533U - A kind of cell recovery device of use for laboratory - Google Patents
A kind of cell recovery device of use for laboratory Download PDFInfo
- Publication number
- CN206553533U CN206553533U CN201720241547.XU CN201720241547U CN206553533U CN 206553533 U CN206553533 U CN 206553533U CN 201720241547 U CN201720241547 U CN 201720241547U CN 206553533 U CN206553533 U CN 206553533U
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- water
- bath room
- electric
- cell recovery
- temperature sensor
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Abstract
The utility model is related to cell biology, specifically disclose a kind of cell recovery device of use for laboratory, including water-bath room, temperature sensor and electric heater unit, water-bath room upper opening, inside is provided with material-putting space, electric heater unit is connected with temperature sensor current output terminal, and temperature sensor current input terminal is connected with power output end, and temperature sensor is electrically connected with temperature controller；Electric and magnetic oscillation plate is provided with above water-bath room, electric and magnetic oscillation plate is contained with water provided with the screens hole for placing cryopreservation tube in material-putting space, and water level is no more than 2cm apart from the distance of electric and magnetic oscillation plate；It is provided with below water-bath room for draining and the outlet pipe equipped with lid.The device is effectively shortened the recovery time, at the same can thermostatic control bath temperature, reduce cell damage unnecessary in resuscitation process, improve the efficiency of cell recovery.
Description
Technical field
The utility model is related to cell biology, the cell recovery device of more particularly to a kind of use for laboratory.
Background technology
It is cryogenic freezing to the important way that cell biological resource is preserved in cell biology, when experiment is needed
When wanting, further take out freeze-stored cell and recovered.Its operating procedure is as follows：1st, burning out for a mug or 1000ml is prepared, it is built-in
2/3 glass 37 DEG C of warm water.2nd, cryopreservation tube is taken out from liquid nitrogen, is immediately placed in warm water and constantly stirs, makes the jelly in cryopreservation tube
Storage was melted within 1 minute.
At present, in laboratory, particularly in laboratory in colleges and universities, most cells recovering experiment is still using tradition at this stage
Method, will need the cell cryopreservation tube of recovery to be placed in the cell bottle of 41 degree of water-baths, and acutely rock body manually, make cell
It is rapid to melt, the effect of " slow speed of freezing is melted ", but one side temperature bad control during due to practical operation are reached, on the other hand manually
Vibration is unable to reach uniform, quick effect, and cell is vulnerable to damage, recovers less efficient.It is thin that fraction laboratory is used
Born of the same parents' recovery device only has temperature control system, and the recovery time is longer, and damage is easily caused to cell.
Utility model content
The purpose of this utility model is that to solve the above problems and research and design goes out a kind of cell of use for laboratory
Recovery device, the device can recovery cell batch simultaneously, and the recovery time is effectively shortened, while being capable of thermostatic control water-bath
Temperature, reduces cell damage unnecessary in resuscitation process, improves the efficiency of cell recovery.
The utility model is achieved through the following technical solutions above-mentioned purpose：
A kind of cell recovery device of use for laboratory, including water-bath room, temperature sensor and electric heater unit, the water-bath
Room upper opening, inside is provided with material-putting space, and the electric heater unit is connected with temperature sensor current output terminal, TEMP
Device current input terminal is connected with power output end, and the temperature sensor is electrically connected with temperature controller；Set above the water-bath room
Electric and magnetic oscillation plate is equipped with, electric and magnetic oscillation plate is contained with water, water provided with the screens hole for placing cryopreservation tube in the material-putting space
Position is no more than 2cm apart from the distance of electric and magnetic oscillation plate；It is provided with below the water-bath room for draining and the outlet pipe equipped with lid.
Moisture is contained in water-bath room, electric heater unit can carry out heat temperature raising, temperature sensor to the moisture in water-bath room
Play temperature control function；Temperature sensor, such as the temperature sensor of thermocouple class, thermocouple respond fast, temperature to temperature change
Degree change causes change in resistance, and then temperature signal is converted into electric signal, and electric signal is flowed into temperature controller, and then controls electricity to add
The break-make of thermal；One temperature range value is set in temperature controller in advance so that at the temperature that temperature sensor is detected
In in the temperature range, electric signal is not transferred to electric heater unit by temperature controller, now, and electric heater unit does not feed water bath indoor water
Divide and heat is provided；If the temperature that temperature sensor is detected is not in the temperature range, electric signal is transferred to electricity by temperature controller
Heater, now, moisture heating in electric heater unit feedwater bathroom；Temperature sensor and temperature controller play thermostatically controlled work
With.Electric and magnetic oscillation plate principle is simple, coil windings lead series diode, and connecting 220V AC powers can work, and pass through card
Position hole is placed on the cryopreservation tube on electric and magnetic oscillation plate, is rocked in the effect of vibration of electric and magnetic oscillation plate, the cell in cryopreservation tube is in
Mobile status, is conducive to cell recovery；Here water level refers to water level to the distance of water-bath room bottom surface, and water level distance
The distance of electric and magnetic oscillation plate refers to that water level subtracts electric and magnetic oscillation plate to the poor absolute value of the distance of water-bath room bottom surface；Water-bath
Room can add water by its upper opening and enter material-putting space, and the outlet pipe then set by lower section discharges water, completes
Change water.
Further, the vibroswitch outside the water-bath room provided with control electric and magnetic oscillation plate current switching in cavity wall and control
The heater switch of electric heater unit current switching processed.
Electric and magnetic oscillation plate and electric heater unit are connected in parallel power supply, respectively right vibroswitch and heater switch control.
Further, the electric heater unit includes heating wire, and the heating wire is no less than two, and subsection setup is in water-bath
Under room in cavity wall.
Using heating wire heat stepwise mode, quick heating.
Further, it is provided with water inlet pipe above the water-bath room.
Due to being additionally provided with electric and magnetic oscillation plate above water-bath room, in order to more easily change water, water inlet pipe is set, water inlet pipe connects
One end connection water source is connect, is introduced the water into water-bath room.
Further, the screens hole is no less than 5.
The presence in multiple screens holes so that cell recovery can be carried out by multiple cryopreservation tubes simultaneously.
Further, the water-bath room is divided into two upper and lower chambers by demarcation strip, respectively upper water-bath room and lower water-bath room；Institute
State and return port is provided with the middle of demarcation strip, being respectively arranged on the left side and the right side one in the demarcation strip is input to the water in lower water-bath room
The water-supply-pipe gone in upper water-bath room, the water-supply-pipe is provided with water circulating pump；The electric heater unit is heating wire, the electric heating
Silk is no less than two, and subsection setup is under lower water-bath room in cavity wall；The temperature sensor is arranged on water-bath room in cavity wall.
Heating wire segmentation is heated to lower water-bath room moisture, now because heat transfer existence time difference, the following water-bath of institute
Room moisture is higher than upper water-bath room moisture temperature；Left and right water circulating pump is opened, the water in lower water-bath room is transported to upper water-bath room
It is interior, it is more than water pressure in lower water-bath room because the water in lower water-bath room has been transported to water pressure in upper water-bath room, upper water-bath room,
Therefore the water of lower section is flowed into lower water-bath room by return port in upper water-bath room, a cyclic process is so completed.It is being circulated throughout
Cheng Zhong, efficiently avoid temperature situation about differing above and below occurring, eliminate above and below temperature a pair of cell recoveries experiments are not caused
Influence.
Further, level sensor is provided with cavity wall on the water-bath room, the level sensor is electrically connected with report
Alarm device.
Level sensor is used to detect liquid level, when liquid level deviate from the liquid level scope of setting, alarm
Device is alarmed, and points out experimenter's water level not reach requirement of experiment.Requirement of experiment said herein is referred to is placed on electricity by cryopreservation tube
When on the screens hole on magnetic vibration plate, the cell in cryopreservation tube will be completely covered in moisture.
Beneficial effect is：Replace artificially rocking cryopreservation tube using electric and magnetic oscillation plate, it is simple to operate, it is ensured that in cryopreservation tube
Frozen stock solution fully melts in a short time, not damaging cells tissue, improves the efficiency of cell recovery；Using temperature sensor and
Temperature controller carries out thermostatic control to moisture in water-bath room, and temperature sensor is temperature sensitive, and measurement temperature is accurate, is effectively prevented from
Excessive temperature damages cell tissue and the temperature too low situation that can not melt in time；Further, using circulation constant temperature water-bath
To carry out thermostatic control, more accurately, self-temperature is just spent more than subzero 100 when being taken out due to cell cryopreservation tube out of liquid nitrogen,
When many solencytes being put into water-bath simultaneously, necessarily causing the water temperature in the region reduces, with conventional method defrosting single tube cell phase
Than extending the time of cell recovery, violating the principle of cell " freezing slowly instant ", increase repeats the risk of crystallization, reduced
Cytoactive, the present apparatus is constantly flowed by circulation constant temperature water-bath, water, is accelerated temperature transmission, be efficiently avoid above-mentioned
The appearance of situation, shortens the cell recovery time, reduces and repeats to crystallize dangerous, raising cytoactive.
Brief description of the drawings
Fig. 1 is a kind of structural representation of the cell recovery device of use for laboratory described in the utility model；
Fig. 2 is a kind of structural representation of the cell recovery device of use for laboratory described in the utility model；
Description of reference numerals is as follows：
1st, water-bath room；101st, upper water-bath room；102nd, lower water-bath room；2nd, electric and magnetic oscillation plate；201st, screens hole；202nd, vibration is opened
Close；3rd, temperature sensor；4th, heating wire；401st, heater switch；5th, demarcation strip；6th, water circulating pump；7th, level sensor；8th, flow back
Hole；9th, water-supply-pipe；10th, demarcation strip；11st, outlet pipe；12nd, water inlet pipe.
Embodiment
The utility model is described in further detail below in conjunction with the accompanying drawings：
Embodiment 1：
A kind of cell recovery device of use for laboratory, including water-bath room 1, temperature sensor 3 and electric heater unit, the water
The upper opening of bathroom 1, inside is provided with material-putting space, is water-bath room 1, the electric heater unit and TEMP, 3 current output terminals
Connection, the current input terminal of temperature sensor 3 is connected with power output end, and the temperature sensor 3 is electrically connected with temperature controller；Institute
State the top of water-bath room 1 and be provided with electric and magnetic oscillation plate 2, electric and magnetic oscillation plate 2 is provided with the screens hole 201 for placing cryopreservation tube, described
Water is contained with water-bath room 1, water level is no more than 2cm apart from the distance of electric and magnetic oscillation plate 2；The lower section of water-bath room 1, which is provided with, to be used for
Draining and the outlet pipe 11 equipped with lid.
Water level is added water into water-bath room 1 at the about 1cm of electric and magnetic oscillation plate 2, is switched on power, sets temperature controller to control temperature
41 ° of degree, when temperature control is to about 41 °, cryopreservation tube is taken out from liquid nitrogen, the screens hole 201 on electric and magnetic oscillation plate 2 is placed on
On, cryopreservation tube while the warm-heat effect in warm water melts medium, and then is completed thin by the effect of vibration of electric and magnetic oscillation plate 2
Born of the same parents recover.
In order to further optimize this programme, lead in the outer chamber wall of water-bath room 1 provided with the electric current of control electric and magnetic oscillation plate 2
Disconnected vibroswitch 202 and the heater switch 401 of control electric heater unit current switching.Electric and magnetic oscillation plate 2 and electric heater unit
It is separately controlled, in use, first opens electric heater unit and preheated；Afterwards when needing to carry out cell recovery, then beat
Vibroswitch 202 is driven, sets two switches to be separately controlled, the energy can be saved.
Optimize this programme in order to further, the electric heater unit includes heating wire 4, and the heating wire 4 is no less than two
Individual, subsection setup is in the cavity wall of water-bath room 1 time.The good quick heating of the heating effect of heating wire 4 of subsection setup.
Optimize this programme in order to further, the top of water-bath room 1 is provided with water inlet pipe 12.The screens hole 201 is many
In 5.5 points by demarcation strip of the water-bath room 1 is two upper and lower chambers, respectively upper water-bath room 101 and lower water-bath room 102；Described
Return port 8 is provided with the middle of demarcation strip 5, the demarcation strip 5 be respectively arranged on the left side and the right side one the water in lower water-bath room 102 is defeated
Enter to the water-supply-pipe 9 in upper water-bath room 101, the water-supply-pipe 9 is provided with water circulating pump 6；The subsection setup of heating wire 4 is in Xia Shui
In the cavity wall of bathroom 102 times 1；The temperature sensor 3 is arranged on water-bath room 1 in cavity wall.Heating wire 4 is segmented to lower water-bath room
1 moisture is heated, now because heat transfer existence time difference, and institute's following water-bath room moisture will than upper water-bath room moisture temperature
It is high；Left and right water circulating pump is opened, the water in lower water-bath room 102 is transported in upper water-bath room 101, due in lower water-bath room 102
Water be transported to upper water-bath room 101, the upper internal water pressure of water-bath room 101 is more than the lower internal water pressure of water-bath room 102, therefore upper water-bath
The water of lower section is flowed into lower water-bath room 102 by return port 8 in room 101, so completes a cyclic process.In cyclic process
In, efficiently avoid temperature situation about differing above and below occurring, eliminate above and below temperature a pair of cell recoveries experiments are not caused
Influence.By the way of circulator bath, further optimization is made to thermostatic control.
Level sensor 7 is provided with cavity wall on the water-bath room 1, the level sensor 7 is electrically connected with warning device.
In order to which guaranteed water level is not less than electric and magnetic oscillation plate 2 more than 2cm, level sensor is set, when not reaching requirement, signal is sent extremely
Warning device is alarmed；So be in order to cryopreservation tube be placed on electric and magnetic oscillation plate 2 when, water can flood completely need recovery
Cellular portions.
Finally, overall among laboratory, the operation of the present apparatus is as follows：First, switch on power, setting temperature controller control
Temperature is 41 ± 1 °, and 1 degree of temperature control error is opened heater switch 401 and water circulating pump 6, when temperature control is at 41 ° or so, beaten
Vibroswitch 202 is driven, the cryopreservation tube taken out from liquid nitrogen is placed on screens hole 201, in the temperature and oscillating plate of water-bath
Vibrate under collective effect, cryopreservation tube inner cell is recovered, after about 15 seconds, cryopreservation tube is taken out；Because having multiple
Screens hole 201, it is possible to which multiple cryopreservation tubes are placed on screens hole 201 while carrying out cell recovery.
By above-mentioned case study on implementation, a kind of cell recovery device of use for laboratory is realized, is replaced using electric and magnetic oscillation plate
Cryopreservation tube artificially is rocked, it is simple to operate, it is ensured that the frozen stock solution in cryopreservation tube fully melts in a short time, not damaging cells group
Knit, improve the efficiency of cell recovery；Thermostatic control, temperature are carried out to moisture in water-bath room using temperature sensor and temperature controller
Sensor for temperature is sensitive, and measurement temperature is accurate, efficiently avoid excessive temperature damage cell tissue and temperature is too low can not
The situation melted in time；Further, thermostatic control is carried out using circulation constant temperature water-bath, more accurately, due to cell cryopreservation
Self-temperature is just in more than subzero 100 degree when pipe takes out out of liquid nitrogen, while when many solencytes are put into water-bath, necessarily causing
The water temperature reduction in the region, compared with conventional method defrosting single tube cell, extends the time of cell recovery, violates cell
The principle of " freezing slowly instant ", increase repeats the risk of crystallization, reduces cytoactive, the present apparatus passes through circulation constant temperature water-bath, water
Constantly flow, accelerate temperature transmission, efficiently avoid the appearance of above-mentioned situation, shorten the cell recovery time, reduce
Repeat to crystallize dangerous, raising cytoactive.
General principle of the present utility model, principal character and advantage has been shown and described above.The technical staff of the industry
It should be appreciated that the utility model is not restricted to the described embodiments, the simply explanation described in above-described embodiment and specification is originally
The principle of utility model, on the premise of the utility model spirit and scope are not departed from, the utility model also has various change
And improvement, these changes and improvements are both fallen within the range of claimed the utility model.Scope is claimed in the utility model
Defined by appended claims and its effect thing.
Claims (7)
1. a kind of cell recovery device of use for laboratory, including water-bath room, temperature sensor and electric heater unit, the water-bath room
Upper opening, inside is provided with material-putting space, it is characterised in that：Temperature sensor described in the electric heater unit connection, institute
State temperature sensor and be electrically connected with temperature controller；Electric and magnetic oscillation plate is provided with above the water-bath room, electric and magnetic oscillation plate is provided with
The screens hole of cryopreservation tube is placed, water is contained with the material-putting space, water level is no more than apart from the distance of electric and magnetic oscillation plate
2cm；It is provided with below the water-bath room for draining and the outlet pipe equipped with lid.
2. a kind of cell recovery device of use for laboratory according to claim 1, it is characterised in that：Outside the water-bath room
The heater switch of vibroswitch and control electric heater unit current switching of the cavity wall provided with control electric and magnetic oscillation plate current switching.
3. a kind of cell recovery device of use for laboratory according to claim 1, it is characterised in that：The electric heater unit
Including heating wire, the heating wire is no less than two, and subsection setup is under water-bath room in cavity wall.
4. a kind of cell recovery device of use for laboratory according to claim 1, it is characterised in that：Above the water-bath room
Provided with water inlet pipe.
5. a kind of cell recovery device of use for laboratory according to claim 1, it is characterised in that：The screens hole is many
In 5.
6. a kind of cell recovery device of use for laboratory according to claim 1, it is characterised in that：The water-bath room is divided
Dividing plate is divided into two upper and lower chambers, respectively upper water-bath room and lower water-bath room；Return port is provided with the middle of the demarcation strip, at described point
Dividing plate is respectively arranged on the left side and the right side one and the water in lower water-bath room is input to the water-supply-pipe gone in upper water-bath room, the water-supply-pipe
It is provided with water circulating pump；The electric heater unit is heating wire, and the heating wire is no less than two, and subsection setup is in lower water-bath room
In lower cavity wall；The temperature sensor is arranged on water-bath room in cavity wall.
7. a kind of cell recovery device of use for laboratory according to claim 1, it is characterised in that：On the water-bath room
Cavity wall is provided with level sensor, and the level sensor is electrically connected with warning device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720241547.XU CN206553533U (en) | 2017-03-13 | 2017-03-13 | A kind of cell recovery device of use for laboratory |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720241547.XU CN206553533U (en) | 2017-03-13 | 2017-03-13 | A kind of cell recovery device of use for laboratory |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206553533U true CN206553533U (en) | 2017-10-13 |
Family
ID=60365648
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720241547.XU Expired - Fee Related CN206553533U (en) | 2017-03-13 | 2017-03-13 | A kind of cell recovery device of use for laboratory |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206553533U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110172400A (en) * | 2019-06-26 | 2019-08-27 | 赛慕特生物工程（上海）有限公司 | A kind of apparatus for the anhydrous dry type recovery of cell |
| CN111763623A (en) * | 2020-07-21 | 2020-10-13 | 四川省人民医院 | Auxiliary activation device for frozen embryos |
| CN113652357A (en) * | 2021-09-16 | 2021-11-16 | 杭州科默斯科技有限公司 | Cell recovery device and cell recovery temperature control method |
-
2017
- 2017-03-13 CN CN201720241547.XU patent/CN206553533U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110172400A (en) * | 2019-06-26 | 2019-08-27 | 赛慕特生物工程（上海）有限公司 | A kind of apparatus for the anhydrous dry type recovery of cell |
| CN111763623A (en) * | 2020-07-21 | 2020-10-13 | 四川省人民医院 | Auxiliary activation device for frozen embryos |
| CN113652357A (en) * | 2021-09-16 | 2021-11-16 | 杭州科默斯科技有限公司 | Cell recovery device and cell recovery temperature control method |
| CN113652357B (en) * | 2021-09-16 | 2024-04-23 | 杭州科默斯科技有限公司 | Cell resuscitating device and cell resuscitating temperature control method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171013 Termination date: 20190313 |