CN111763623A - Auxiliary activation device for frozen embryos - Google Patents
Auxiliary activation device for frozen embryos Download PDFInfo
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- CN111763623A CN111763623A CN202010715166.7A CN202010715166A CN111763623A CN 111763623 A CN111763623 A CN 111763623A CN 202010715166 A CN202010715166 A CN 202010715166A CN 111763623 A CN111763623 A CN 111763623A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/02—Means for providing, directing, scattering or concentrating light located outside the reactor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
Abstract
The utility model provides an auxiliary activation device of freezing embryo, relates to medical equipment technical field, includes that inside has the casing that holds the chamber, and the freezing embryo that holds in the wheat pipe sets up in holding the intracavity, is provided with the light source on the internal face of casing, and the light source is to freezing embryo outgoing low-intensity light. The auxiliary activation device for the frozen embryo can be used as an auxiliary activation means for the frozen embryo, and the survival rate of the frozen embryo recovery is improved, so that the clinical pregnancy rate of the frozen embryo recovery is improved.
Description
Technical Field
The invention relates to the technical field of medical equipment, in particular to an auxiliary activation device for a frozen embryo.
Background
The embryo freezing method is a method of filling the embryo and freezing liquid into a wheat tube, enabling the embryo to be static through two cooling modes of slow (embryo at 2-3 days) and fast (blastocyst at 5-6 days), and storing the embryo in a liquid nitrogen environment at 196 ℃ below zero, and implanting the embryo into a uterine cavity after unfreezing in a natural period or an artificial period, so that the chance of conception is increased, and the method is the only mature method for storing the fertility function so far.
Although embryos can be dormant for a long time in an ultralow temperature environment, the process of thawing, thawing and activating frozen embryos is required to be performed quickly so as to improve the survival rate of the frozen embryos in thawing. In the prior art, the embryo is unfrozen by adopting a quick unfreezing method, and the unfreezing steps comprise: 1. taking out the straw from the liquid nitrogen tank, volatilizing at room temperature for 30s, and then putting into a water bath at 30 ℃ for 30 s; 2. cutting off a tampon at the upper end of a straw, putting the cut cotton into a straw pipettor, cutting off the sealing position at the lower end of the straw, transferring the embryo in the straw into unfreezing T1 liquid (0.5 mol/L1, 2-Propanediol (1,2-Propanediol for short, PROH), 0.2mol/L sucrose, and Phosphate Buffer Solution (PBS) containing 20% Human Serum Albumin (Human Serum Albumin for short, HAS)) and staying for 7 min; 3. transferring embryo into unfreezing T2 solution (0.33mol/L PROH, 0.2mol/L sucrose, PBS solution containing 20% HAS), and standing for 7 min; 4. transferring embryo into unfreezing T3 solution (0.1mol/L PROH, 0.2mol/L sucrose, PBS solution containing 20% HAS), and standing for 7 min; 5. transferring embryo into thawing T4 solution (PBS solution containing 20% HAS), and standing for 7 min; 6. and transferring the embryo into a well balanced culture solution, washing for three times, transferring into the next culture solution, and placing in an incubator for continuous culture for 2 hours for embryo transplantation.
However, the rapid thawing method mainly depends on the natural recovery of embryos, the survival rate of the recovery of the frozen embryos is low, and the clinical pregnancy rate of the recovery of the frozen embryos is less than 50%.
Disclosure of Invention
The invention aims to provide an auxiliary activation device for frozen embryos, which can be used as an auxiliary activation means for frozen embryos, and can improve the survival rate of the recovery of the frozen embryos, so that the clinical pregnancy rate of the recovery of the frozen embryos is improved.
The embodiment of the invention is realized by the following steps:
the embodiment of the invention provides an auxiliary activation device for frozen embryos, which comprises a shell with an accommodating cavity inside, wherein the frozen embryos accommodated in a straw are arranged in the accommodating cavity, a light source is arranged on the inner wall surface of the shell, and the light source emits low-intensity light to the frozen embryos. The auxiliary activation device for the frozen embryo can be used as an auxiliary activation means for the frozen embryo, and the survival rate of the frozen embryo recovery is improved, so that the clinical pregnancy rate of the frozen embryo recovery is improved.
Optionally, in a preferred embodiment of the present invention, a placing platform is further disposed in the accommodating chamber, and the placing platform has a liquid containing tank for containing thawing liquid, so that the frozen embryo is thawed in the thawing liquid.
Optionally, in a preferred embodiment of the present invention, the object placing table is made of a light-transmitting material, the light source is disposed at the bottom of the housing, and the light source emits light toward the object placing table.
Optionally, in a preferred embodiment of the present invention, the wavelength of the light source is 500 to 10000nm, and the power density of the light source is 0.01 to 30mw/cm2。
Optionally, in a preferred embodiment of the present invention, the light sources are point light sources, and the point light sources are uniformly distributed on an inner wall surface of the housing; or the light source is a linear light source which is arranged on the inner wall surface of the shell in parallel; or, the light source is a surface light source.
Optionally, in a preferred embodiment of the present invention, a turntable is further disposed inside the housing, the frozen embryo is disposed on the turntable, and the auxiliary activating device for freezing an embryo further includes a driving assembly, which is in transmission connection with the turntable and is used for driving the turntable to rotate.
Optionally, in a preferred embodiment of the present invention, the casing is made of an opaque material, and/or a light absorbing layer is disposed on an inner wall surface of the casing.
Optionally, in a preferred embodiment of the present invention, an electric heating wire is further disposed inside the casing, the electric heating wire is connected to a power interface through a wire, the power interface is disposed on an outer wall surface of the casing, and a temperature relay is further disposed on the wire for controlling a working state of the electric heating wire.
Optionally, in a preferred embodiment of the present invention, a switch for controlling the on/off of the circuit and an indicator light for displaying the working state of the heating wire are further disposed on the outer wall surface of the housing.
Optionally, in a preferred embodiment of the present invention, a temperature sensor is further disposed inside the casing, a display is further disposed on an outer wall surface of the casing, the device for assisting in activating frozen embryos further includes a controller electrically connected to the temperature sensor and the display, respectively, and the controller controls the display to display a corresponding temperature value according to a received temperature signal detected by the temperature sensor.
The embodiment of the invention has the beneficial effects that:
the auxiliary activation device for the frozen embryo comprises a shell, wherein a containing cavity is arranged in the shell, the frozen embryo originally contained in a straw is moved into the containing cavity for unfreezing, an external environment suitable for unfreezing and reviving of the frozen embryo can be created in the containing cavity closed in the shell through setting of environmental conditions such as unfreezing liquid and the like in the shell, on the basis, a light source arranged on the inner wall surface of the shell is controlled to emit low-intensity light towards the frozen embryo, so that the frozen embryo is excited to have cell activity under the irradiation of the low-intensity light, the revival probability of the embryo in the whole process of unfreezing the frozen embryo is improved, therefore, the auxiliary activation device can be used as an auxiliary activation means for the frozen embryo, the survival rate of reviving of the frozen embryo is improved, the embryo planting rate, the clinical pregnancy rate and the birth rate are improved, and the hatching rate of the blasto, increase the chance of embryo implantation, thereby finally improving the clinical pregnancy rate of frozen embryo resuscitation.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 is a schematic structural diagram of an auxiliary activation device for freezing embryos according to an embodiment of the present invention;
FIG. 2 is a second schematic structural diagram of an auxiliary activation device for freezing embryos according to an embodiment of the present invention;
FIG. 3 is a third schematic structural diagram of an auxiliary activation device for freezing embryos according to an embodiment of the present invention;
FIG. 4 is a fourth schematic view of the auxiliary activation device for freezing embryos according to the embodiment of the present invention.
Icon: 100-auxiliary activation means; 10-a housing; 20-a containment chamber; 30-a light source; 40-a placement table; 41-groove; 42-a limit rib; 200-freezing the embryo; 300-liquid containing groove.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
In the description of the present invention, it should be noted that the terms "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings or orientations or positional relationships that are conventionally placed when the products of the present invention are used, and are used only for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the devices or elements to be referred to must have specific orientations, be constructed in specific orientations, and operate, and thus, should not be construed as limiting the present invention.
In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; either directly or indirectly through intervening media, or may be internal to both elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Examples
Referring to fig. 1 to 4, the present embodiment provides an auxiliary activation device 100 for a frozen embryo 200 (hereinafter referred to as an auxiliary activation device 100), which includes a housing 10 having an accommodating cavity 20 therein, the frozen embryo 200 accommodated in a straw is disposed in the accommodating cavity 20, a light source 30 is disposed on an inner wall surface of the housing 10, and the light source 30 emits low-intensity light to the frozen embryo 200. The auxiliary activation device 100 of the frozen embryo 200 can be used as an auxiliary activation means of the frozen embryo 200, and the recovery survival rate of the frozen embryo 200 is improved, so that the recovery clinical pregnancy rate of the frozen embryo 200 is improved.
It should be noted that therapeutic light sources (including laser and LED) applied in medicine can be generally classified into two types: weak light and strong light. Because of the different biological action mechanisms of strong light and weak light, the purposes and methods of clinical application are different. In the medical field, the intensity of light is not measured by the physical parameters of light itself (such as power and energy), but is distinguished by the intensity of biological effect produced after the light acts on biological tissues. After the biological tissue is irradiated by light, if irreversible damage to the biological tissue is directly caused, the light at the irradiated surface is called strong light; if the injury is not directly caused (i.e. the forward, beneficial stimulation and stimulation is only generated to the biological tissue), the light is called weak light, which is also called low-intensity light.
By continuously irradiating the frozen embryo 200 with the low-intensity light, various stimulation effects on the frozen embryo 200 can be realized, which mainly comprises the steps of obviously improving the embryo planting rate, the clinical pregnancy rate and the parturition rate, also obviously improving the hatching rate of the blastocyst, increasing the chance of implantation of the embryo, finally improving the clinical pregnancy rate and the like, so that the activity of the revived frozen embryo 200 is improved to a certain extent. The low-intensity light may include He-Nc laser (wavelength 632.8nm), GaAlAs laser (wavelength 820nm, 830nm), GaAs laser (wavelength 904nm), Nd: YAG laser (wavelength 1064nm), etc., different types of lasers are selected, and the corresponding lasers for generating the laser light are different, and those skilled in the art can select the low-intensity light according to actual needs, and there is no specific limitation here.
It should be noted that the above-mentioned setting of the frozen embryo 200 accommodated in the straw in the accommodating cavity 20 means that the straw originally stored in a liquid nitrogen tank in frozen form is filled with the frozen embryo 200 and the freezing fluid, and when the process of thawing, thawing and activating the frozen embryo 200 is started, the straw filled with the frozen embryo 200 and the freezing fluid is taken out of the liquid nitrogen tank, and then the frozen embryo 200 is thawed by combining the prior art fast thawing method of the frozen embryo 200 under the assisting activation effect of the assisting activation device 100.
Specifically, the thawing step of thawing the frozen embryo 200 by combining the rapid thawing method of the frozen embryo 200 in the prior art with the auxiliary activation of the auxiliary activation device 100 includes:
s100, taking out the straw from the liquid nitrogen tank, volatilizing the straw at room temperature for 30s, and then putting the straw into a water bath at 30 ℃ for 30 s;
s200, cutting off a cotton plug part at the upper end of a straw, loading the cotton plug part into a straw pipette, cutting off the sealing position at the lower end of the straw, transferring the frozen embryo 200 in the straw into unfreezing T1 liquid (0.5 mol/L1, 2-Propanediol (1,2-Propanediol for short), 0.2mol/L sucrose, Phosphate Buffer Solution (PBS) containing 20% Human Serum Albumin (Human Serum Albumin for short), and placing the frozen embryo 200 and the unfreezing T1 liquid into an auxiliary activation device 100 to irradiate for 7min through a light source 30;
s300, transferring the frozen embryo 200 to unfreezing T2 liquid (0.33mol/L PROH, 0.2mol/L sucrose, PBS liquid containing 20% HAS), putting the frozen embryo 200 and the unfrozen T2 liquid into an auxiliary activation device 100, and irradiating for 7min through a light source 30;
s400, transferring the frozen embryo 200 to unfreezing T3 liquid (0.1mol/L PROH, 0.2mol/L sucrose, PBS liquid containing 20% HAS), putting the frozen embryo 200 and the unfrozen T3 liquid into an auxiliary activation device 100, and irradiating for 7min through a light source 30;
s500, transferring the frozen embryo 200 into unfreezing T4 liquid (PBS liquid containing 20% HAS), putting the frozen embryo 200 and the unfrozen T4 liquid into an auxiliary activation device 100, and irradiating for 7min by a light source 30;
s600, transferring the frozen embryo 200 into a well-balanced culture solution, washing for three times, transferring into the next culture solution, and placing in an incubator to continue to culture for 2 hours for embryo transplantation.
It will be understood by those skilled in the art that the above-described transfer of frozen embryos 200 into various thawing solutions means that the thawing solutions themselves must be previously contained in a container, such as a cup-shaped vessel, a bottle-shaped vessel, etc. in the prior art, or a culture vessel separately designed for thawing and activating frozen embryos 200, such as the fluid container 300 proposed in the present application. For example, after the preparation of the various thawing solutions, the embryos 200 may be placed in a plurality of containers 300, and when thawing the frozen embryos 200, the frozen embryos 200 may be moved into the containers 300 containing the corresponding thawing solutions in sequence, and then the containers 300 may be placed into the accommodating cavities 20 and irradiated by the light source 30. The preparation of the thawing solution, the irradiation duration and the like are designed by referring to the prior art, and in the actual process, a person skilled in the art can carry out certain adjustment according to the thawing condition of the frozen embryo 200.
As shown in fig. 1-4, the housing 10 should be closed during use of the auxiliary activation device 100, but need not be closed or sealed, and can be generally hinged or hinged on one side to open when desired to facilitate access to the frozen embryo 200. Regarding the closing manner of the casing 10, there may be various forms, for example, any one of the side surfaces or the top surface enclosing the casing 10 is selected to be a hinged panel, and the hinged panel is opened when the frozen embryo 200 is to be taken and placed, wherein, as shown in fig. 1, the panel may be a plate body in an integral structure, that is, one side of the plate body is fixed to the casing 10 by a hinge, and the other side of the plate body can rotate around the hinge to open or close the casing 10, as shown in fig. 2 to 4, the panel may also be two plate bodies in a split structure, that is, the two plate bodies are fixed to the casing 10 by a hinge at the sides far away from each other, and the two plate bodies can rotate around the respective hinges to open or close the casing 10. Of course, in other embodiments, the cover plate on the top of the housing 10 may be designed to be opened and closed directly, or opened directly when the frozen embryo 200 is to be handled.
In addition, different irradiation modes can be selected for the irradiation of the light source 30, for example, continuous irradiation of the light source 30, which means that the light output is continuous and stable, and pulsed irradiation of the light source 30, which means that the light irradiation is intermittent. Both of the above two irradiation methods can be used to irradiate the frozen embryo 200 with the light source 30, and those skilled in the art should be able to make a reasonable choice according to the actual situation, and are not limited herein.
As described above, the auxiliary activation device 100 includes the housing 10, the housing 10 has the accommodating chamber 20 therein, the frozen embryo 200 originally accommodated in the straw is moved into the accommodating chamber 20 for thawing, and an external environment suitable for thawing and reviving of the frozen embryo 200 can be created in the accommodating chamber 20 in the housing 10 by setting an environmental condition such as a thawing solution in the housing 10, and on this basis, the light source 30 provided on the inner wall surface of the housing 10 is controlled to emit low-intensity light toward the frozen embryo 200, so that the frozen embryo 200 is irradiated by the low-intensity light to excite the cell activity thereof, thereby improving the revival probability of the embryo in the whole thawing process of the frozen embryo 200, and therefore, the auxiliary activation device 100 can be used as an auxiliary activation means for the frozen embryo 200 to improve the reviving survival rate of the frozen embryo 200, thereby improving the embryo planting rate, the pregnancy clinical rate and the parturition rate, the hatching rate of the blastocyst can be obviously improved, the chance of embryo implantation is increased, and the recovery clinical pregnancy rate of the frozen embryo 200 is finally improved.
As shown in fig. 3 and 4, in the present embodiment, a platform 40 is further disposed in the accommodating cavity 20, the platform 40 has a liquid container 300, and the liquid container 300 is used for containing a thawing solution to thaw the frozen embryo 200 in the thawing solution.
First, in the present embodiment, the extension direction of the placing table 40 may be parallel to the height direction of the housing 10, that is, the placing table 40 may be a T-shaped structure (as shown in fig. 3), a column-shaped structure, a circular table-shaped structure, or a trapezoid-shaped structure extending from the bottom of the housing 10 toward the top of the housing 10, so as to provide the placing and supporting function for the frozen embryo 200. Of course, in other embodiments, as shown in fig. 4, the extension direction of the placing table 40 may also be perpendicular to the height direction of the housing 10, that is, the placing table 40 may be a plate-shaped structure extending from the rear of the housing 10 to the front of the housing 10, as long as the table top of the placing table 40 can provide a placing and supporting function for the frozen embryo 200.
Secondly, as shown in fig. 3 and 4, the top surface of the placement platform 40 generally extends in a direction parallel to the bottom surface of the housing 10, and a groove 41 with a certain depth may be further formed on the top surface of the placement platform 40 by being recessed inward, or a plurality of limiting ribs 42 may be further formed on the top surface of the placement platform 40 by being protruded outward, so that an optimal placement position for placing the frozen embryo 200 is formed by enclosing the plurality of limiting ribs 42, and thus, the irradiation coverage effect of the light source 30 on the frozen embryo 200 placed in the optimal placement position can be optimal in the use process of the auxiliary activation device 100. It should be noted that when the placing table 40 is not disposed inside the housing 10, the frozen embryo 200 can be directly placed on the bottom plate of the housing 10 to be accommodated in the accommodating cavity 20, as shown in fig. 1 and 2, and the above-mentioned manner of forming the optimal placement position for placing the frozen embryo 200 by providing the groove 41 or the limiting rib 42 on the placing table 40 is also applicable to the bottom plate of the housing 10.
As shown in fig. 3 and 4, in the present embodiment, the placing table 40 is made of a light-transmitting material, in this case, the light source 30 may be disposed not only on the side surface or the top portion of the casing 10, but also on the bottom portion of the casing 10, and the light source 30 emits light toward the placing table 40, as long as the low-intensity light emitted through the light source 30 can irradiate the frozen embryo 200. Light can be irradiated on the frozen embryo 200 through the transparent material placing table 40, so that one side of the frozen embryo 200 close to the table top of the placing table 40 can be irradiated by the light source 30. The transparent material may be glass, plastic, etc.
In the present embodiment, the wavelength of the light source 30 is 500-10000 nm, especially 600-1100 nm, and the power density of the light source 30 is 0.01-30 mw/cm2. Illustratively, the wavelength is typically 632.8nm, 820nm, 830nm, or 904 nm; the power density is mostly less than 10mw/cm2(few may be more than 10 mw/cm)2) (ii) a The waveform is continuous wave or pulse wave (1-4000 Hz). It is to be noted that the wavelength and power density of the light source 30 can be reasonably selected and designed by those skilled in the art according to practical situations, and are not particularly limited herein.
Optionally, the light sources 30 are point light sources, and the point light sources are uniformly distributed on the inner wall surface of the casing 10; alternatively, the light source 30 is a linear light source, and the linear light source is disposed in parallel on the inner wall surface of the housing 10; alternatively, the light source 30 is a surface light source.
It should be noted that, firstly, as shown in fig. 2, when the light source 30 is a point light source, the point light source may be uniformly arranged on the inner wall surface of the housing 10, so that the frozen embryo 200 can be uniformly irradiated, and the damage of the frozen embryo 200, which may be caused by the irradiation of light concentrated at some point, can be avoided.
Secondly, as shown in fig. 1, 3 and 4, when the light source 30 is a linear light source, the linear light source may be disposed in parallel on the inner wall surface of the housing 10, and two adjacent linear light sources may be disposed at equal intervals, so that the extending directions of the two adjacent linear light sources are parallel and the intervals are equal. Generally, the number of line light sources can be set to 2-6 to optimize the effect of assisting in activating the frozen embryo 200. When the number of the linear light sources is less, the range of the linear light sources irradiating the frozen embryo 200 may be smaller, and the purpose of improving the survival rate of the frozen embryo 200 can not be achieved; when the number of the line light sources is large, the heat generated when the line light sources irradiate the frozen embryo 200 is large, which may adversely affect the development of the embryo.
Thirdly, when the light source 30 is a surface light source, the surface light source may be disposed on any inner wall surface of the housing 10, and a plurality of surface light sources may be disposed adjacently or oppositely to increase the irradiation range of the frozen embryo 200.
Further, in this embodiment, a rotating disc is further disposed inside the housing 10, the frozen embryo 200 is disposed on the rotating disc, and the auxiliary activation device 100 for freezing the embryo 200 further includes a driving assembly, which is in transmission connection with the rotating disc and is used for driving the rotating disc to rotate.
It should be noted that the inside of the housing 10 is provided with a rotating disc, and the frozen embryo 200 is arranged on the rotating disc, that is, the rotating disc is used as a support for the frozen embryo 200 to provide a placing and supporting function for the frozen embryo 200. Drive assembly is connected with the carousel transmission to when making drive assembly drive carousel rotate, can drive freezing embryo 200 at the casing 10 internal rotation, thereby further improve freezing embryo 200 and receive irradiant uniform degree, and then improve freezing embryo 200's the survival rate of recovering from life or consciousness.
In this embodiment, the casing 10 is made of opaque material, and/or a light-absorbing layer is disposed on the inner wall of the casing 10 to prevent the interference of the illumination of the external environment to the photo-biological effect of the frozen embryo 200.
It should be noted that, firstly, the housing 10 is made of opaque material, and/or a light absorbing layer is disposed on an inner wall surface of the housing 10, which specifically includes the following three conditions:
in the first case, the housing 10 is made of opaque material, for example, the housing 10 can be made of metal, alloy, ceramic, etc., so as to achieve the above-mentioned purpose.
In the second case, a light absorbing layer is disposed on the inner wall surface of the housing 10, and for example, the inner wall surface of the housing 10 may be coated with a light absorbing material, thereby achieving the above-mentioned object.
In the third case, the housing 10 is made of opaque material, and the light absorbing layer is disposed on the inner wall surface of the housing 10, that is, under the precondition that no contradiction exists, the features included in the first two cases are freely combined in the third case, thereby achieving the above-mentioned purpose. Illustratively, the housing 10 is made of a metal material and the inner wall surface of the housing 10 is coated with a light absorbing material, or the housing 10 is made of an alloy material and the inner wall surface of the housing 10 is coated with a light absorbing material, or the housing 10 is made of a ceramic material and the inner wall surface of the housing 10 is coated with a light absorbing material.
Second, when the light absorbing layer is disposed on the inner wall surface of the housing 10, the light source 30 can be disposed where the low-intensity light emitted from the light source 30 can be absorbed when the low-intensity light irradiates the inner wall surface of the housing 10, so that the low-intensity light emitted from the light source 30 can avoid the loss of light energy caused by the light source 30 emitting outside the housing 10.
Since the frozen embryo 200 is originally stored in the ultralow temperature environment of 196 ℃ below zero for resting storage, the temperature environment of the frozen embryo 200 can be raised to some extent during the activation and recovery process of the frozen embryo 200 to assist in activating the frozen embryo 200.
Specifically, in this embodiment, a heating wire is further disposed inside the casing 10, the heating wire is connected to a power interface through a wire, the power interface is disposed on an outer wall surface of the casing 10, and a temperature relay is further disposed on the wire for controlling an operating state of the heating wire.
It should be noted that, regardless of the installation position of other components, the heating wires may be uniformly arranged on each wall surface of the housing 10 to uniformly heat the inside of the housing 10, thereby increasing the ambient temperature inside the housing 10. When the blocking influence of other components exists, the heating wire can be properly avoided. The power interface is disposed on the outer wall surface of the casing 10 to communicate with an external power source through the power interface, so as to provide power for heating the heating wire. A certain temperature threshold, for example, a temperature suitable for resuscitation and activation of the frozen embryo 200, such as 30 ℃, 35 ℃, 37 ℃ and the like, is preset in the temperature relay in advance, so that when the heating wire is in a heating working state and reaches the preset temperature threshold, the heating wire enters a power-off protection state, the heating wire is not heated continuously any more, and the influence of overhigh temperature on the activity of the frozen embryo 200 is avoided.
In this embodiment, a switch for controlling the on/off of the circuit and an indicator light for displaying the working state of the heating wire are further disposed on the outer wall surface of the housing 10.
It should be noted that a switch for controlling the on/off of the circuit is further disposed on the outer wall surface of the casing 10, so that the working state of the heating wire can be controlled not only by the on/off of the power interface and the external power source, but also by the on/off of the switch. An indicator light for displaying the working state of the heating wire is further disposed on the outer wall surface of the housing 10, so that the working state of the heating wire can be visually observed, and the influence caused when the switch is opened or closed by mistake is avoided.
Further, in this embodiment, a temperature sensor is further disposed inside the casing 10, a display is further disposed on the outer wall surface of the casing 10, the auxiliary activation device 100 for freezing the embryo 200 further includes a controller electrically connected to the temperature sensor and the display, respectively, and the controller controls the display to display a corresponding temperature value according to a received temperature signal detected by the temperature sensor, so that the ambient temperature inside the casing 10 can be intuitively monitored in real time.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. The auxiliary activation device for the frozen embryo is characterized by comprising a shell with an accommodating cavity inside, wherein the frozen embryo accommodated in a straw is arranged in the accommodating cavity, a light source is arranged on the inner wall surface of the shell, and the light source emits low-intensity light to the frozen embryo.
2. The device for assisting activation of a frozen embryo according to claim 1, wherein a platform is further disposed in the receiving chamber, the platform having a liquid containing tank for containing a thawing solution for thawing the frozen embryo in the thawing solution.
3. The auxiliary activating device for freezing embryo according to claim 2, wherein the platform is made of light-transmitting material, the light source is disposed at the bottom of the housing, and the light source emits light toward the platform.
4. The auxiliary activation device for frozen embryo according to claim 1, wherein the wavelength of the light source is 500-10000 nm, and the power density of the light source is 0.01-30 mw/cm2。
5. The auxiliary activating device for freezing embryo according to claim 1, wherein the light source is a point light source, and the point light sources are uniformly distributed on the inner wall surface of the shell; or the light source is a linear light source which is arranged on the inner wall surface of the shell in parallel; or, the light source is a surface light source.
6. The device for assisting in activating frozen embryos according to claim 1, wherein a turntable is further disposed inside the housing, the frozen embryos are disposed on the turntable, and the device for assisting in activating frozen embryos further comprises a driving assembly, and the driving assembly is in transmission connection with the turntable and is used for driving the turntable to rotate.
7. The device for assisting in activating a frozen embryo according to claim 1, wherein the housing is made of a light-impermeable material, and/or a light-absorbing layer is disposed on an inner wall surface of the housing.
8. The auxiliary activation device for freezing embryo as claimed in claim 1, wherein a heating wire is further disposed inside the casing, the heating wire is connected to a power interface through a wire, the power interface is disposed on the outer wall surface of the casing, and a temperature relay is further disposed on the wire for controlling the operating state of the heating wire.
9. The auxiliary activating device for freezing embryo according to claim 8, wherein the outer wall of the casing is further provided with a switch for controlling the on/off of the circuit and an indicator light for displaying the working status of the electric heating wire.
10. The device for assisting in activating frozen embryos according to claim 8, wherein a temperature sensor is further disposed inside the casing, a display is further disposed on an outer wall surface of the casing, the device for assisting in activating frozen embryos further comprises a controller electrically connected to the temperature sensor and the display, respectively, and the controller controls the display to display a corresponding temperature value according to a received temperature signal detected by the temperature sensor.
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| CN202010715166.7A CN111763623A (en) | 2020-07-21 | 2020-07-21 | Auxiliary activation device for frozen embryos |
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| CN202010715166.7A CN111763623A (en) | 2020-07-21 | 2020-07-21 | Auxiliary activation device for frozen embryos |
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| CN111763623A true CN111763623A (en) | 2020-10-13 |
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| CN202010715166.7A Pending CN111763623A (en) | 2020-07-21 | 2020-07-21 | Auxiliary activation device for frozen embryos |
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