CN210929356U - Closed ovary tissue refrigerating device - Google Patents
Closed ovary tissue refrigerating device Download PDFInfo
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- CN210929356U CN210929356U CN201921775119.0U CN201921775119U CN210929356U CN 210929356 U CN210929356 U CN 210929356U CN 201921775119 U CN201921775119 U CN 201921775119U CN 210929356 U CN210929356 U CN 210929356U
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Abstract
The utility model discloses a closed ovary tissue refrigerating plant, including ovary tissue support, seal bag and a save section of thick bamboo, ovary tissue support is used for placing the ovary tissue stripping and slicing of separation, and the ovary tissue stripping and slicing of separation is placed in the loading trough, can guarantee effectively that ovary tissue keeps better morphological structure at freezing in-process, and the through-hole of tank bottom can increase the area of contact of ovary tissue and external medium, improves cooling and programming rate, and then improves the survival rate after the freezing thawing of ovary tissue. The sealing bag is used for sealing the ovarian tissue scaffold, so that the ovarian tissue can be prevented from being directly contacted with liquid nitrogen in the freezing and storing processes, and the cross contamination among samples and the damage and damage of ozone generated by the liquid nitrogen to the DNA of the ovarian tissue are prevented. The storage cylinder is used for placing the sealed ovarian tissue scaffold, so that the ovarian tissue scaffold can be effectively prevented from being mixed with other individual samples in the storage process, and meanwhile, the collision and breakage of the sealing bag can be safely prevented.
Description
Technical Field
The application belongs to the technical field of reproductive medicine and assisted reproduction, and particularly relates to a closed ovarian tissue freezing device.
Background
Female fertility preservation generally takes three modes: cryopreservation of embryonic, egg or ovarian tissue. Since the first birth of healthy infants after the frozen and thawed ovarian tissue is transplanted in situ in 2004 in the world, nearly 130 infants are estimated to be born after the frozen and thawed ovarian tissue is transplanted.
At present, the cryopreservation of human ovarian tissues is carried out by using an open freezing device. The freezing preservation of the open ovarian tissue, wherein the ovarian tissue is directly contacted with liquid nitrogen in the freezing and preserving processes, has potential cross infection risks among samples, and the liquid nitrogen can damage sample DNA. Therefore, it is urgent to produce an effective ovarian tissue freezing apparatus.
Disclosure of Invention
In order to solve the technical problem, the utility model provides a closed ovary tissue refrigerating plant can avoid ovary tissue sample at freezing and direct and liquid nitrogen contact of save in-process to the ozone that has prevented cross contamination and liquid nitrogen production between the sample is to the damage and the destruction of ovary tissue DNA.
The technical scheme adopted for realizing the purpose of the utility model is that the closed ovary tissue refrigerating device comprises an ovary tissue bracket, a sealing bag used for sealing the ovary tissue bracket and a storage barrel used for placing the ovary tissue bracket;
the ovary tissue bracket comprises a handheld part and a loading part, wherein the loading part comprises more than 1 loading groove, and more than 1 through hole is formed in the groove bottom of the loading groove.
Preferably, the ovarian tissue scaffold is of an integrated structure, the whole structure is long-strip-shaped, one end of the ovarian tissue scaffold is a handheld part, and the rest part of the ovarian tissue scaffold is provided with a loading groove to form the loading part.
Preferably, the loading part comprises more than 2 loading grooves, and the more than 2 loading grooves are distributed in parallel along the length direction of the ovarian tissue scaffold.
Preferably, the sealed bag is integrally long-strip-shaped, and one end of the sealed bag is open and is used for placing the ovarian tissue stent.
Preferably, a sealing strip is arranged at the opening of the sealing bag.
Preferably, the wall of the storage barrel is provided with a loading and unloading opening.
Preferably, the loading/unloading opening is an inclined opening formed by cutting a part of the cylindrical wall on the cylindrical wall in an inclined manner with respect to the cylindrical axis.
Preferably, the length of the inclined opening is 0.5-1 times of the length of the ovarian tissue scaffold.
Preferably, the preservation cylinder is a cylinder with one end open and the other end closed.
Preferably, the bottom end of the inclined opening faces the closed end of the cylinder.
According to the above technical scheme, the utility model provides a closed ovary tissue refrigerating plant, including ovary tissue support, sealing bag and a save section of thick bamboo, wherein: the ovary tissue bracket is used for placing the ovary tissue blocks in the separation, and comprises a handheld portion and a loading portion, the handheld portion is convenient for a person to hold the ovary tissue blocks by hands, the operation is convenient, the loading portion comprises a loading groove with a plurality of groove bottom openings, the ovary tissue blocks in the separation are placed in the loading groove, the ovary tissue can be effectively guaranteed to keep a better morphological structure in the freezing process, through holes in the groove bottoms can increase the contact area between the ovary tissue and external media (freezing media, liquid nitrogen, thawing media, buffer solution and thawing solution), the cooling and heating speed is improved, and the survival rate of the ovary tissue after freezing and thawing is further improved. The sealed bag is used for packaging the ovarian tissue scaffold, and researches show that ionizing radiation generated by ozonolysis in liquid nitrogen can cause cell DNA damage, has the risk of virus transmission and has potential harm to the frozen and stored ovarian tissue. The sealed bag can prevent the ovary tissues from directly contacting with liquid nitrogen in the freezing and storing processes, thereby preventing the cross contamination among samples and the damage and damage of ozone generated by the liquid nitrogen to the DNA of the ovary tissues. The storage cylinder is used for placing the sealed ovarian tissue scaffold, so that the ovarian tissue scaffold can be effectively prevented from being mixed with other individual samples in the storage process, and meanwhile, the collision and breakage of the sealing bag can be safely prevented.
The closed ovarian tissue freezing device isolates the direct contact between the ovarian tissue and liquid nitrogen, can reduce the damage of the liquid nitrogen to the ovarian tissue and the cross infection among the ovarian tissues of different individuals, and can become the development trend of the cryopreservation of the ovarian tissue in the future in clinical application.
Drawings
FIG. 1 is a schematic diagram of an embodiment of the present invention showing an overall structure of a closed ovarian tissue freezing apparatus;
FIG. 2 is a schematic structural diagram of the ovarian tissue scaffold of FIG. 1;
FIG. 3 is a schematic view of the structure of the sealed pouch of FIG. 1;
FIG. 4 is a schematic view of the construction of the retention cartridge of FIG. 1;
description of reference numerals: 1-ovarian tissue scaffold, 11-handheld part, 12-loading part, 13-loading groove and 14-through hole; 2-sealing bag, 21-sealing strip; 3-preservation cylinder, 31-loading and taking port, 32-opening bottom.
Detailed Description
In order to make the present application more clearly understood by those skilled in the art to which the present application pertains, the following detailed description of the present application is made with reference to the accompanying drawings by way of specific embodiments.
In an embodiment of the present invention, a closed ovarian tissue freezing apparatus is used for cryopreservation of ovarian tissue in liquid nitrogen. Referring to fig. 1, the device comprises an ovarian tissue scaffold 1, a sealing bag 2 for packaging the ovarian tissue scaffold 1, and a storage barrel 3 for placing the ovarian tissue scaffold 1; the structure of each of the above-described members is described in detail below:
the ovary tissue bracket 1 is used for placing an isolated ovary tissue section, the ovary tissue bracket 1 comprises a handheld part 11 and a loading part 12, and the handheld part 11 is convenient for being held by hands and is convenient to operate; the loading part 12 comprises more than 1 loading groove, and the separated ovary tissue blocks are placed in the loading grooves 13.
Referring to fig. 2, in the present embodiment, the ovarian tissue scaffold 1 is integrally formed by injection molding, and has a long shape as a whole, one end of which is a planar structure and forms a holding portion 11, and the rest of which is provided with a loading slot 13 and forms a loading portion 12. The ovarian tissue scaffold 1 shown in fig. 2 is provided with 4 loading grooves 13, the 4 loading grooves 13 are distributed in parallel along the length direction of the ovarian tissue scaffold 1, and multiple ovarian tissue cut pieces can be loaded at one time (it should be noted that the same ovarian tissue scaffold 1 can only load one individual isolated ovarian tissue, and the multiple loading is for ensuring the survival amount of the ovarian protocells in the ovarian tissue and meeting the requirement of assisted reproduction). The separated ovary tissue blocks are placed in the loading groove 13, so that the ovary tissue can be effectively kept in a better morphological structure in the freezing process.
Referring to fig. 2, more than 1 through hole 14 is formed at the bottom of the loading groove, the through holes 14 are densely distributed at the bottom of the loading groove, the through holes 14 can increase the contact area of the ovarian tissue and an external medium, the cooling and heating speed is increased, and the survival rate of the ovarian tissue after freezing and thawing is further increased.
The sealing bag 2 is used for sealing the ovarian tissue scaffold, and can prevent the ovarian tissue from directly contacting with liquid nitrogen in the freezing and storing processes, so that cross contamination among samples and damage of ozone generated by the liquid nitrogen to the DNA of the ovarian tissue are prevented.
Referring to fig. 3, the sealing bag 2 is a strip shape, one end of the sealing bag is open, and is used for placing the ovarian tissue stent 1, the sealing bag is a common plastic film bag, and the opening can be sealed by a heating sealing machine.
In a preferred embodiment, a sealing strip 21 is arranged at the opening of the sealing bag 2, and the sealing bag 2 can be closed by kneading the sealing strip 21 after the ovary tissue stent 1 is placed, as shown in fig. 3.
The preservation cylinder 3 is used for placing the sealed ovarian tissue scaffold 1, so that the ovarian tissue scaffold can be effectively prevented from being mixed with other individual samples in the preservation process, and meanwhile, the collision and breakage of the sealing bag can be safely prevented. The preservation cylinder 3 is a cylinder with one end open and the other end closed, and the sealed ovarian tissue scaffold 1 can be put into the preservation cylinder through the opening.
Referring to fig. 4, in a preferred embodiment, the wall of the storage cylinder 3 is provided with an assembling and disassembling opening 31, specifically, the assembling and disassembling opening 31 is an inclined opening formed by obliquely cutting a part of the cylinder wall on the cylinder wall relative to the cylinder axis, a bottom end 32 (i.e. the deepest part of the opening) of the inclined opening faces the closed end of the cylinder, and the axial length of the inclined opening is 0.5-1 times of the length of the ovarian tissue scaffold 1, so that the encapsulated ovarian tissue scaffold 1 can be more conveniently taken out.
Because the placement of the ovarian tissue scaffold 1 is completed in liquid nitrogen, compared with the placement of the encapsulated ovarian tissue scaffold 1 from the end opening, the storage and the taking out of the ovarian tissue scaffold 1 through the side loading and taking-out port 31 are more convenient to operate.
The closed ovary tissue freezing device provided by the utility model is used for freezing and storing ovary tissues, the whole height of the ovary tissue scaffold is 2.5-4 mm, the whole length is 120-150 mm, and the whole width is 10-15 mm; the depth of the loading groove is 1.5-2.5 mm, and the length of the loading groove is 20-25 mm; the aperture of the groove bottom is 1.5-2.5 mm, the pitch of the holes is 3-6 mm, and the specific operation process is as follows:
firstly, a vitrification freezing process:
(1) the isolated ovarian tissue is cleaned by normal saline or related culture solution, then placed into a plastic culture dish (60.0mm by 60.0mm), and loose connective tissue on the inner side of the ovarian cortex (only the cortex in the ovarian tissue contains the ovarian protocells, so the inner connective tissue needs to be removed before freezing, and the ovarian tissue is ensured to be effectively frozen) is removed by scissors.
(2) Cutting the ovarian cortex tissue without the inner connective tissue into small pieces with the size of 1.0cm x 1.0cm, putting the small pieces into an ovarian tissue freezing balanced solution (V1), balancing for 20 minutes at room temperature, and then transferring the balanced small pieces of the ovarian cortex into an ovarian tissue vitrification frozen solution (V2) for processing for 10 minutes;
(3) the small ovarian cortex blocks are placed in a loading groove of a marked ovarian tissue scaffold to be laid evenly, then the small ovarian cortex blocks are sleeved with a sealing bag, and all air bubbles are extruded out (the air bubbles can influence the cooling and heating speed, and can also influence the cryopreservation effect, so that the air bubbles need to be removed). After confirming that no air bubble exists, a heat sealing machine or a direct extrusion sealing strip is used for sealing the sealing bag, so that the content of the sealing bag is completely isolated from the outside.
(4) Quickly putting the sealed ovarian tissue scaffold into a liquid nitrogen tank, transferring the sealed ovarian tissue scaffold into a marked storage cylinder in liquid nitrogen, and then transferring the sealed ovarian tissue scaffold into a liquid nitrogen storage tank for storage.
Secondly, unfreezing:
(1) the ovarian tissue scaffolds were rapidly transferred from liquid nitrogen to a container containing 37 ℃ physiological saline or phosphate buffer, the outer sealed bag was thawed and separated from the ovarian tissue mass of the ovarian tissue scaffolds, and the ovarian tissue scaffolds (and ovarian tissue) were immediately transferred to thawing solution T1(1mol/L sucrose solution) (room temperature) and allowed to stand for 5 minutes (e.g., in a test tube containing 5 ml of thawing solution T1).
(2) The ovarian tissue on the ovarian tissue scaffold was detached from the loading well by forceps and kept in thawing solution T2(0.5mol/L sucrose solution) (room temperature) for 8 minutes (for example, placed in a test tube containing 5 ml of thawing solution T2).
(3) The ovarian tissue was transferred with forceps to a thawing solution T3(0.25mol/L sucrose solution) (room temperature) and kept for 8 minutes (for example, in a test tube containing 5 ml of thawing solution T3).
(4) The ovarian tissue was transferred with forceps to a thawing solution T4 (physiological saline) (room temperature) for 8 minutes (for example, placed in a test tube containing 5 ml of the thawing solution T4).
(5) The ovarian tissue is transferred to a medium containing normal saline or phosphate buffer (room temperature) with forceps to complete in situ or ex situ or to perform tissue culture within two hours.
Through the embodiment, the utility model has the following beneficial effect or advantage:
1) the utility model provides a closed ovary tissue freezing device, which comprises an ovary tissue bracket, a sealing bag and a storage barrel, wherein the ovary tissue bracket can effectively ensure that the ovary tissue keeps a better morphological structure in the freezing process; the sealed bag can prevent the ovary tissue sample from directly contacting with liquid nitrogen in the freezing and storing processes, thereby preventing cross contamination among samples and damage and destruction of ozone generated by the liquid nitrogen to the DNA of the ovary tissue; the preservation cylinder is used for freezing preservation of the ovarian tissue scaffold, can effectively prevent the ovarian tissue scaffold from being mixed with other individual samples in the preservation process, and can safely prevent the sealing bag from collision and rupture.
2) The utility model provides a closed ovary tissue refrigerating plant, ovary tissue support's the dense through-hole of loading groove tank bottom, the through-hole can increase the area of contact of ovary tissue and external medium, improves cooling and programming rate, and then improves the survival rate after the freezing of ovary tissue unfreezes.
3) The utility model provides a closed ovary tissue refrigerating plant sets up the slope opening for a section of thick bamboo axis slope on the section of thick bamboo wall of a save section of thick bamboo, because placing of ovary tissue support is accomplished in the liquid nitrogen, compares in the ovary tissue support of putting into after the encapsulation from the tip opening, deposits and takes out ovary tissue support more convenient operation through side slope opening.
While the preferred embodiments of the present application have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. Therefore, it is intended that the appended claims be interpreted as including preferred embodiments and all alterations and modifications as fall within the scope of the application.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present application without departing from the spirit and scope of the application. Thus, if such modifications and variations of the present application fall within the scope of the claims of the present application and their equivalents, the present application is intended to include such modifications and variations as well.
Claims (10)
1. A closed ovary tissue freezing device is characterized in that: comprises an ovarian tissue scaffold, a sealing bag used for packaging the ovarian tissue scaffold, and a preservation cylinder used for placing the ovarian tissue scaffold;
the ovary tissue bracket comprises a handheld part and a loading part, wherein the loading part comprises more than 1 loading groove, and more than 1 through hole is formed in the groove bottom of the loading groove.
2. The closed ovarian tissue freezing apparatus of claim 1 wherein: the ovary tissue bracket is of an integrated structure, is integrally long-strip-shaped, and is provided with a handheld part at one end and loading grooves in the rest part to form the loading part.
3. The closed ovarian tissue freezing apparatus of claim 2, wherein: the loading part comprises more than 2 loading grooves, and the more than 2 loading grooves are distributed in parallel along the length direction of the ovarian tissue scaffold.
4. The closed ovarian tissue freezing apparatus of claim 2, wherein: the whole sealing bag is in a strip shape, and one end of the sealing bag is opened and is used for placing the ovarian tissue stent.
5. The closed ovarian tissue freezing apparatus of claim 4 wherein: and a sealing strip is arranged at the opening of the sealing bag.
6. The closed ovarian tissue freezing apparatus of any of claims 1 to 5 wherein: the wall of the storage barrel is provided with a loading and unloading opening.
7. The closed ovarian tissue freezing apparatus of claim 6, wherein: the loading and unloading opening is an inclined opening formed by obliquely cutting a part of the cylinder wall relative to the cylinder axis on the cylinder wall.
8. The closed ovarian tissue freezing apparatus of claim 7, wherein: the length of the inclined opening is 0.5-1 time of the length of the ovarian tissue scaffold.
9. The closed ovarian tissue freezing apparatus of claim 7 or 8, wherein: the preservation cylinder is a cylinder with one open end and the other closed end.
10. The closed ovarian tissue freezing apparatus of claim 9, wherein: the bottom end of the inclined opening faces the closed end of the cylinder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921775119.0U CN210929356U (en) | 2019-10-22 | 2019-10-22 | Closed ovary tissue refrigerating device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921775119.0U CN210929356U (en) | 2019-10-22 | 2019-10-22 | Closed ovary tissue refrigerating device |
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| CN210929356U true CN210929356U (en) | 2020-07-07 |
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| CN201921775119.0U Active CN210929356U (en) | 2019-10-22 | 2019-10-22 | Closed ovary tissue refrigerating device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111903666A (en) * | 2020-09-03 | 2020-11-10 | 浙江省畜牧技术推广与种畜禽监测总站(浙江省农业机械试验鉴定推广总站) | Hu sheep semen freezing tube and thawing apparatus |
| WO2021129312A1 (en) * | 2019-12-24 | 2021-07-01 | 上海明悦医疗科技有限公司 | Carrier, vacuumizing device and tissue cryopreservation system |
-
2019
- 2019-10-22 CN CN201921775119.0U patent/CN210929356U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021129312A1 (en) * | 2019-12-24 | 2021-07-01 | 上海明悦医疗科技有限公司 | Carrier, vacuumizing device and tissue cryopreservation system |
| CN111903666A (en) * | 2020-09-03 | 2020-11-10 | 浙江省畜牧技术推广与种畜禽监测总站(浙江省农业机械试验鉴定推广总站) | Hu sheep semen freezing tube and thawing apparatus |
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