CN111903666A

CN111903666A - Hu sheep semen freezing tube and thawing apparatus

Info

Publication number: CN111903666A
Application number: CN202010914558.6A
Authority: CN
Inventors: 章晓炜; 宋美娥; 蔡美红; 任永业; 高慧; 王海燕
Original assignee: Zhejiang Provincial Animal Husbandry Technology Extension And Breeding Livestock Monitoring Station
Current assignee: Zhejiang Provincial Animal Husbandry Technology Extension And Breeding Livestock Monitoring Station
Priority date: 2020-09-03
Filing date: 2020-09-03
Publication date: 2020-11-10
Anticipated expiration: 2040-09-03
Also published as: CN111903666B

Abstract

The invention discloses a Hu sheep semen freezing pipe which comprises a pipe body, a pipe cover matched with an opening at the top of the pipe body and an accommodating cavity arranged in the pipe body, wherein a clamping convex part is arranged on the pipe cover, the pipe body comprises an outer pipe and an inner pipe, annular accommodating cavities are formed between the outer pipe and the inner pipe at intervals, a cavity is arranged on the inner pipe, and the lower end of the cavity is communicated with the outside. Compared with the traditional method that liquid nitrogen only contacts with the outer wall of the tank body, the method increases the contact area between the pipe body and the liquid nitrogen and the contact area between the inner wall of the pipe body and semen in the freezing process, accelerates the freezing speed of the semen, reduces the damage of freezing to the semen and improves the survival rate of the semen.

Description

Hu sheep semen freezing tube and thawing apparatus

Technical Field

The invention relates to the technical field of Hu sheep breeding, in particular to a Hu sheep semen freezing pipe and a thawing device.

Background

The Hu sheep is one of important domestic animals in Taihu plain and is a first-level protective local livestock and poultry variety in China. In Hu sheep physique, there are no corners on male and female, long and narrow head, humped nose bridge, large and drooping ears, long and thin neck, long and narrow trunk, flat back and waist, slight drooping abdomen, flat and round tail, upwarping tail tip, thin and high limbs. White quilt hair, thick, thin and short abdomen hair, and strong physique. The Hu sheep is a rare white lamb skin sheep variety, has the excellent properties of precocity, four-season estrus, two fetuses in one year, more lambs in each fetus, good lactation performance, quick growth and development, ideal meat production performance after improvement, high temperature and high humidity resistance and the like, is distributed in Taihu areas in China, is fed with the Chinese lamb skin sheep variety in houses in the last year, and is attractive in pattern of the small lake lamb skin which is peeled 1-2 days after delivery and is famous for the life.

The semen freezing preservation and artificial insemination technology plays a very important role in the production of modern animal husbandry, the artificial insemination by using the frozen semen can be free from the limitation of time, regions, varieties and individuals, the feeding quantity of the male livestock is greatly reduced, the production cost is reduced, the utilization rate of the male livestock with excellent varieties is improved, and the economic benefit of the breeding industry is improved. Meanwhile, the technology has important significance in the aspects of variety introduction, hybridization improvement, reproductive performance improvement, disease control and protection of genetic diversity. Sheep sperms are weaker than chicken sperms and cattle sperms, and damage is easily caused in the freezing and unfreezing processes.

Disclosure of Invention

The invention aims to solve the problems in the prior art and provides a Hu sheep semen freezing pipe and a thawing device which are beneficial to reducing the damage to sperms.

The purpose of the invention can be realized by the following technical scheme: the utility model provides a hu sheep seminal fluid cryovial, includes the body, with body open-top department matched with tube cap and locate the cavity that holds in the body, be equipped with the centre gripping convex part on the tube cap, the body includes outer tube and inner tube, the interval forms annular chamber that holds between outer tube and the inner tube, be equipped with the cavity on the inner tube, the cavity lower extreme is linked together with the external world.

When the sperm freezing device is used for freezing, liquid nitrogen enters the cavity and contacts with the outer wall of the inner tube, compared with the traditional method that the liquid nitrogen only contacts with the outer wall of the tank body, the contact area between the pipe body and the liquid nitrogen and the contact area between the inner wall of the pipe body and the semen are increased in the freezing process, the freezing speed of the semen is accelerated, the damage of freezing to the semen is reduced, and the survival rate of the semen is improved; in the transportation process of the freezing pipe, the semen is in the annular accommodating cavity, so that the semen is not prone to violent shaking along with shaking of a vehicle, and once the semen is violently shaken, the semen is easy to damage and the survival rate of the semen is influenced; semen is deposited in this freezing intraductal back, because semen is in the annular intracavity that holds, semen can laminate to be one kind as far as on the body wall body and follow the even state of spreading out of pipe wall, whether there is impurity in the operating personnel of being convenient for observes semen.

As a preferred scheme of the invention, a plurality of connecting pipes are connected between the outer pipe and the inner pipe, a flow channel is arranged in each connecting pipe, the outer pipe is provided with a first opening communicated with one end of the flow channel, and the inner pipe is provided with a second opening communicated with the other end of the flow channel; when the freezing pipe is placed in liquid nitrogen for freezing, the liquid nitrogen flows into the flow channel to be contacted with the inner wall of the connecting pipe, the outer wall of the connecting pipe is contacted with semen in the pipe body, the contact area of the semen and the liquid nitrogen is further increased, the freezing speed of the semen is accelerated, the damage of the freezing to the semen is reduced, the survival rate of the semen is improved, and hot gas or hot water flows into the flow channel to be contacted with the inner wall of the connecting pipe when the freezing pipe is heated and thawed, so that the thawing efficiency of the semen is improved; and the setting of a plurality of connecting pipes makes the bulk strength of body improve, is difficult for taking place to damage at the in-process of transportation, is convenient for the save and the transportation of seminal fluid.

As a preferable scheme of the invention, the cross section of the connecting pipe is in an inverted triangle shape, and the inverted triangle shape ensures that when an operator unfreezes semen and pours outwards, the semen in the containing cavity flows downwards and is cut off by the sharp corner of the connecting pipe, so that the usage rate of the semen is effectively prevented from being reduced due to the fact that the semen is adhered to the connecting pipe when the semen flows, the semen in the containing cavity is discharged outwards as much as possible, and the utilization rate is improved.

As a preferable aspect of the present invention, the inner tube is provided with a plurality of first arc-shaped portions along a circumferential direction thereof; a plurality of second arc-shaped parts are arranged on the outer pipe along the circumferential direction of the outer pipe; the setting up of first arc portion and second arc portion makes the area of contact who holds semen and liquid nitrogen or hot water steam of intracavity further improve, helps improving the freezing and the efficiency of unfreezing of semen, and compares traditional cylindrical body and be difficult for taking place when keeping flat on the laboratory bench and roll, places more stably, reduces the damage risk of body.

As a preferred scheme of the invention, two clamping grooves are arranged on the clamping convex part, the two clamping grooves are respectively symmetrically arranged on two sides of the clamping convex part, and the clamping grooves are obliquely and obliquely inclined from outside to inside; tweezers freeze and unfreeze embryo/gamete long-time operation in-process at vitrification, owing to pass in and out the liquid nitrogen repeatedly, the front end of tweezers can freeze, and the sawtooth part of freezing back tweezers front end is covered by ice, and the tweezers surface becomes very smooth, through the setting in both sides centre gripping groove for when the centre gripping convex part is got to tweezers clamp, thereby the tweezers both sides can be gone into respectively and apply certain effort to the centre gripping inslot that corresponds and mention the cryovial, stability when having improved tweezers centre gripping cryovial.

A thawing device of a Hu sheep semen freezing pipe comprises a base and a thawing part arranged on the base, wherein a plurality of thawing grooves for pipe bodies to be inserted are formed in the thawing part, thawing rods inserted into cavities are arranged in the thawing grooves, and a heating driving piece for heating the thawing part and the thawing grooves is arranged on the base; need when thawing the semen in the cryovial, insert the cryovial to the inslot that unfreezes, the stick that unfreezes simultaneously inserts to the cavity of cryovial, the cryovial can play limiting displacement to the cryovial with the inslot wall that unfreezes, thereby make this thawing apparatus can use in step at the in-process of transportation, with the condition that uses to other places after some needs unfreeze of adaptation, and unfreeze in step when the transportation, very big save the preparation time of prophase, in time utilize it after the semen unfreezes, help improving the survival rate of semen.

As a preferable scheme of the present invention, the thawing part has a first thawing chamber therein, the thawing rod has a second thawing chamber therein, the first thawing chamber and the second thawing chamber are communicated, and the first thawing chamber and the second thawing chamber are filled with a thawing solution; according to the invention, the unfreezing liquid is heated by the heating driving piece, and then the heat is transferred to the freezing pipe through the unfreezing liquid to unfreeze the semen, compared with the method of directly transferring the heat of the heating driving piece to the freezing pipe, the unfreezing liquid can absorb and uniformly transfer the heat of the heating driving piece to the freezing pipe to unfreeze the semen, the temperature change range of the freezing liquid is smaller, the temperature can be conveniently controlled within a certain range, and the situations that the semen is unfrozen incompletely due to too low temperature and the semen is inactivated due to too high temperature can be avoided

As the preferred scheme of the invention, the unfreezing part and the unfreezing rod are both made of silica gel materials; the silica gel material enables the unfreezing part and the unfreezing rod to have good heat conducting capacity, so that heat of unfreezing liquid can be conveniently and quickly transferred to the freezing pipe, meanwhile, the silica gel material has good deformation capacity, when the freezing pipe is inserted into the unfreezing groove, the unfreezing part can deform to wrap the freezing pipe, the heat conducting capacity is improved, and meanwhile, the unfreezing rod can also deform to improve the fit degree of the unfreezing rod and the inner wall of the inner pipe, so that the heat conducting capacity of the unfreezing rod is improved; and the portion of unfreezing that the silica gel material was made has very good buffer capacity when filling with unfreezing liquid, even unfreeze at the in-process of transportation, when haulage vehicle took place great vibration, the vibration impact most that the cryovial received can be cushioned by the portion of unfreezing to make the cryovial unfreeze under a comparatively stable condition, guarantee the survival rate of the semen after arriving the destination, and guarantee that the semen can in time be used.

As a preferable scheme of the invention, the volume of the accommodating cavity is 5-15 ml; the volume undersize can lead to the space utilization of cryovial to hang down excessively, makes the cost increase substantially, and the volume is too big at the in-process of cryovial transportation, and the seminal fluid rocks in the cryovial easily, and drops more easily when operating personnel takes with tweezers.

Compared with the prior art, the liquid nitrogen freezing tank has the advantages that the traditional liquid nitrogen is only contacted with the outer wall of the tank body, the contact area between the pipe body and the liquid nitrogen and the contact area between the inner wall of the pipe body and semen are increased in the freezing process, the semen freezing speed is increased, the damage of freezing to the semen is reduced, and the survival rate of the semen is improved.

Drawings

FIG. 1 is a schematic structural diagram of a first embodiment of the present invention;

FIG. 2 is a first cross-sectional view of a first embodiment of the present invention;

FIG. 3 is a partial schematic view of FIG. 2 at A;

FIG. 4 is a partial schematic view of FIG. 2 at B;

FIG. 5 is a second cross-sectional view of the first embodiment of the present invention;

FIG. 6 is a schematic structural diagram of a second embodiment of the present invention;

FIG. 7 is a sectional view of a second embodiment of the present invention;

in the figure, 1, a tube body; 2. a tube cover; 3. an accommodating chamber; 4. a clamping protrusion; 5. an outer tube; 6. an inner tube; 7. An accommodating chamber; 8. a cavity; 9. a connecting pipe; 10. a flow channel; 11. a first opening; 12. a second opening; 13. A first arcuate portion; 14. a second arcuate portion; 15. a clamping groove; 16. a base; 17. a thawing section; 18. a thawing tank; 19. a defrosting rod; 20. heating the driving member; 21. a first thawing chamber; 22. a second thawing chamber.

Detailed Description

The following are specific embodiments of the present invention and are further described with reference to the drawings, but the present invention is not limited to these embodiments.

Example one

As shown in fig. 1-5, a hu sheep semen freezing tube and thawing device comprises a tube body 1, a tube cap 2 matched with the top opening of the tube body 1 and a containing cavity 3 arranged in the tube body 1, wherein the tube body 1 is provided with an opening for filling semen, the tube cap 2 is installed at the opening of the tube body 1 through threads to seal the containing cavity 3, the tube cap 2 is provided with a clamping convex part 4, the clamping convex part 4 is a cylinder which is vertically and upwardly protruded on the upper end surface of the tube cap 2, the clamping convex part 4 and the tube cap 2 are integrally formed, the tube body 1 comprises an outer tube 5 and an inner tube 6, the outer tube 5 and the inner tube 6 are both cylindrical, the inner tube 6 is provided with a plurality of first arc parts 13 along the circumferential direction, the outer tube 5 is provided with a plurality of second arc parts 14 along the circumferential direction, the outer tube 5 is sleeved outside the inner tube 6, and the annular containing cavity 7 is formed between the outer tube 5 and the, the lower part of the sealing cover is communicated with the outside to form a cavity 8, and the lower end of the inner tube 6 is hermetically connected with the lower end of the outer tube 5 through a bottom cover, so that the leakage of the semen in the accommodating cavity 7 is prevented.

Specifically, a plurality of connecting pipes 9 are connected between the outer pipe 5 and the inner pipe 6, the connecting pipes 9 are arranged at equal intervals along the height direction of the pipe body 1, 4 groups of the connecting pipes are arranged at equal intervals along the circumferential direction of the pipe body 1, the connecting pipes 9 are horizontally arranged between the outer pipe 5 and the inner pipe 6, a flow channel 10 is arranged in the connecting pipes 9, the flow channel 10 extends along the length direction of the connecting pipes 9, a first opening 11 communicated with one end of the flow channel 10 is arranged on the outer pipe 5, a second opening 12 communicated with the other end of the flow channel 11 is arranged on the inner pipe 6, the flow channel 10 is communicated with the outer space of the outer pipe 5 through the first opening 11, the flow channel 10 is,

in this embodiment, the cross section of the connecting pipe 9 is an inverted triangle, when the semen is poured into the inverted pipe body 1, the sharp corner of the connecting pipe 9 can divide the semen, so that the semen is prevented from being adhered to the connecting pipe 9, and the utilization rate of the semen is improved.

In this embodiment, in order to prevent that the operator from taking place the landing when pressing from both sides centre gripping convex part 4 with tweezers clamp, be equipped with two centre gripping grooves 15 on the centre gripping convex part 4, centre gripping convex part 4 both sides are located to two centre gripping grooves 15 symmetries respectively, and centre gripping grooves 15 is outside-in slant tilt up setting.

Example two

As shown in fig. 6-7, a thawing device for a hu sheep semen freezing pipe comprises a base 16 and a thawing part 17 arranged on the base 16, wherein the base 16 is square, the thawing part 17 is a cylindrical structure fixed on the upper end surface of the base 16, the thawing part 17 is provided with a plurality of thawing grooves 18 for inserting the pipe body 1, the thawing grooves 18 are formed by downward recessing the upper end surface of the thawing part 17, the groove diameter is the same as the diameter of the outer pipe 5, a thawing rod 19 inserted into the cavity 8 is arranged in the thawing groove 18, the thawing rod 19 is formed by upward protruding the value of the bottom wall of the thawing groove 18, a first thawing cavity 21 is arranged in the thawing part 17, a second thawing cavity 22 is arranged in the thawing rod 19, the first thawing cavity 21 is communicated with the second thawing cavity 22, thawing liquid is filled in both the first thawing cavity 21 and the second thawing cavity 22, the base 16 is provided with a heating driving member 20 for heating the thawing liquid, the heating driving member 20 is made of a commercially available heating rod, the thawing part 17 and the thawing rod 19 are made of silica gel, and the volume of the accommodating cavity 7 is 5-15ml, preferably 10 ml.

The specific embodiments described herein are merely illustrative of the spirit of the invention. Various modifications or additions may be made to the described embodiments or alternatives may be employed by those skilled in the art without departing from the spirit or ambit of the invention as defined in the appended claims.

Although terms are used more often herein, the possibility of using other terms is not excluded. These terms are used merely to more conveniently describe and explain the nature of the present invention; they are to be construed as being without limitation to any additional limitations that may be imposed by the spirit of the present invention.

Claims

1. The utility model provides a hu sheep seminal fluid cryostraw which characterized in that: including body (1), with body (1) top opening part matched with tube cap (2) and locate body (1) in hold chamber (3), be equipped with centre gripping convex part (4) on tube cap (2), body (1) includes outer tube (5) and inner tube (6), the interval forms annular chamber (7) that holds between outer tube (5) and inner tube (6), be equipped with cavity (8) on inner tube (6), cavity (8) lower extreme is linked together with the external world.

2. The hu-sheep semen freezing pipe as claimed in claim 1, wherein: be connected with a plurality of connecting pipes (9) between outer tube (5) and inner tube (6), be equipped with runner (10) in connecting pipe (9), be equipped with first opening (11) that are linked together with runner (10) one end on outer tube (5), be equipped with second opening (12) that are linked together with the runner (11) other end on inner tube (6).

3. The hu-sheep semen freezing pipe as claimed in claim 2, wherein: the cross section of the connecting pipe (9) is in an inverted triangle shape.

4. The hu-sheep semen freezing pipe as claimed in claim 1, wherein: a plurality of first arc-shaped parts (13) are arranged on the inner pipe (6) along the circumferential direction of the inner pipe; a plurality of second arc-shaped parts (14) are arranged on the outer pipe (5) along the circumferential direction of the outer pipe.

5. The hu-sheep semen freezing pipe as claimed in claim 1, wherein: be equipped with two centre gripping grooves (15) on centre gripping convex part (4), two centre gripping groove (15) symmetry respectively locates centre gripping convex part (4) both sides, and centre gripping groove (15) are outside-in slant slope and incline upward and set up.

6. The thawing device of the Hu sheep semen freezing pipe as claimed in claim 1, wherein: including base (16) and locate thawing portion (17) on base (16), be equipped with a plurality of confession bodys (1) male thawing groove (18) on thawing portion (17), be equipped with in thawing groove (18) and be used for inserting stick (19) that unfreeze to in cavity (8), be equipped with on base (16) and be used for heating driving piece (20) to thawing portion (17) and thawing groove (18) heating.

7. The thawing device of the Hu sheep semen freezing pipe as claimed in claim 1, wherein: the thawing device is characterized in that a first thawing cavity (21) is formed in the thawing part (17), a second thawing cavity (22) is formed in the thawing rod (19), the first thawing cavity (21) is communicated with the second thawing cavity (22), and thawing liquid is filled in the first thawing cavity (21) and the second thawing cavity (22).

8. The thawing device of the Hu sheep semen freezing pipe as claimed in claim 7, wherein: the unfreezing part (17) and the unfreezing rod (19) are made of silica gel materials.

9. The thawing device of the Hu sheep semen freezing pipe as claimed in claim 1, wherein: the volume of the accommodating cavity (7) is 5-15 ml.