CN216931621U - Freezing save set of biomaterial - Google Patents
Freezing save set of biomaterial Download PDFInfo
- Publication number
- CN216931621U CN216931621U CN202123364524.3U CN202123364524U CN216931621U CN 216931621 U CN216931621 U CN 216931621U CN 202123364524 U CN202123364524 U CN 202123364524U CN 216931621 U CN216931621 U CN 216931621U
- Authority
- CN
- China
- Prior art keywords
- biological material
- pipeline
- freezing
- oil
- cryopreservation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Abstract
The utility model provides a freezing and storing device for biological materials, which comprises a water-in-oil droplet forming chip and a freezing carrying rod, wherein the water-in-oil droplet forming chip comprises a main biological material flowing pipeline, a freezing and storing agent branch pipeline and an oil phase material branch pipeline which are arranged in the chip; one end of the main pipeline is a biomaterial inlet, and the other end of the main pipeline is a biomaterial oil drop outlet; a cryopreservation agent branch pipeline communicating port and an oil phase material branch pipeline communicating port are arranged on the main pipeline in sequence along a biological material flowing path; one end of the cryopreservation agent branch pipeline is communicated with the biological material main circulation pipeline through a cryopreservation agent branch pipeline communication port, and the other end of the cryopreservation agent branch pipeline is a cryopreservation agent inlet; one end of the oil phase material branch pipeline is communicated with the main pipeline through an oil phase material branch pipeline communication port, and the other end of the oil phase material branch pipeline is an oil phase material inlet; the freezing carrying rod is provided with a carrying groove for accommodating biological material oil drops. The cryopreservation device can improve the sealing property and firmness of biological material preservation.
Description
Technical Field
The utility model relates to a device for freezing and preserving biological materials, in particular to a device for generating and freezing and preserving biological materials, which comprises a water-in-oil droplet forming chip and a freezing carrying rod.
Background
In the field of fertility technology, some biological materials such as somatic cells, sperm, ova, day 1-6 embryos, etc. often require cryopreservation. For example, in tube infant therapy, many of the ova removed patients are not suitable for fresh embryo implantation, and therefore, the fertilized ova or blastocysts are frozen to be implanted preferentially when the patients return to a suitable implantation state. In addition, in order to prevent the failure of one implantation, the ovum which is taken out at the same time and is not used for a long time is frozen and preserved, the situation that the ovum needs to be taken out again for a female is prevented, and the like, all of which relate to the freezing and the unfreezing of the embryo.
Freezing embryos is the only established method for preserving fertility. Freezing embryo and freezing liquid are filled into freezing tube, and the embryo can be made to stand and stored in liquid nitrogen at 196 ℃ below zero by two cooling modes of slow (embryo at 2-3 days) and fast (blastocyst at 5-6 days). The artificial uterine tube is implanted into a uterine cavity after being thawed in a natural cycle or an artificial cycle, so that the opportunity of conception of a woman ready for pregnancy is increased. According to statistics, the clinical pregnancy rate of frozen embryo resuscitation is 48.28%, and the clinical pregnancy rate of blastocyst frozen resuscitation is 63.48%.
Fast freezing is more advantageous than slow freezing. Conventional freezing refers to a slow method of slowly cooling the embryo until it eventually freezes, and the major problem with this approach is the formation of ice crystals during freezing that can produce damage to the embryo. The rapid method is also called as a vitrification freezing method, and the vitrification freezing method can avoid the occurrence of ice crystals, improve the success rate of embryo implantation and effectively protect embryos from being damaged in the freezing process. The vitrification freezing method is gradually replacing the conventional method.
The freezing carrying rod is an essential consumable material for the embryo freezing link and is closely related to the freezing-recovery effect of the embryo. Currently, most reproductive centers mainly use three types of vitrification freezing carrying rods, open, semi-closed and closed. The open type carrying rod and the semi-closed type carrying rod enable embryos to be in direct contact with liquid nitrogen when the embryos are frozen and stored, cross contamination among the embryos can exist, or potential safety hazards exist on the embryos due to embryo contamination caused by microbial contamination existing in the liquid nitrogen. For the reproductive center, the safety of the embryo is very important, so the use of a closed freezing carrying rod is especially necessary.
CN112674076A discloses a closed type freezing carrying rod, which comprises an inner tube, an outer tube, a tube cap, an air bag, a tapered closing-in and a soft closing ring. When in use, the air bag is matched with the conical closing-in to absorb or lead out sperms and ova; after the absorption, the outer tube is moved, a part of the soft sealing ring arranged at the tapered closing opening is pushed forwards by utilizing the appearance, and the soft sealing ring is inwards extruded and contracted by matching with the appearance, so that the tapered closing opening is sealed; otherwise, the soft sealing ring is opened and reset. However, in the freezing rod, the rod body and the cap material of the rod are not consistent, the surface tension is not uniform, a gap is easily caused by expansion with heat and contraction with cold, and the 'sealing' in the true sense is difficult to realize.
CN209882898U discloses a vitrified freezing straw, which comprises a carrying rod and a sealing sleeve made of the same material, wherein a stepped plug structure is arranged on the carrying rod, so as to realize the sealing between the carrying rod and the sleeve. However, even if the material of the rod body of the carrying rod and the material of the closing cap are the same, the complete close fit of the rod body and the closing cap is difficult to ensure, and the leakage risk still exists.
In addition, the carrying rod in the prior art is not provided with a position for fixing and placing the embryo, but is placed at a certain position of the carrying rod according to the habit of a doctor, and the position of the embryo needs to be searched during observation; there are some loading rods that are simply provided with pits as loading grooves for placing embryos, however, since the loading rods are usually long, the operation needs to be carefully and smoothly performed, otherwise, the embryos are easily ejected or knocked off due to the inclination or vibration of the loading rods, and there is a bad result that the embryos cannot be retrieved when being thawed.
SUMMERY OF THE UTILITY MODEL
It is an object of the present invention to provide an apparatus for cryopreservation of biological materials.
The inventor of the present invention has proposed a novel method for cryopreservation of a biological material, wherein the biological material is a somatic cell, sperm, ovum or embryo (D1-D6) or plant cell, etc. from a human or an animal, the method comprising: encapsulating the biological material to be frozen in an oil phase by adopting a water-in-oil technology to form biological material oil drops; and (3) placing the biological material oil drops on a freezing carrying rod, covering inert oil on the biological material oil drops for sealing, and then performing liquid nitrogen freezing preservation.
In order to preserve the biological material more conveniently, the utility model provides a cryopreservation device for the biological material, which comprises a water-in-oil droplet formation chip and a freezing carrying rod, wherein:
the water-in-oil droplet forming chip comprises a main biological material circulation pipeline, a cryopreservation agent branch pipeline and an oil phase material branch pipeline which are arranged in the chip; wherein: one end of the biological material main flow pipeline is a biological material inlet, and the other end of the biological material main flow pipeline is a biological material oil drop outlet; a cryopreservation agent branch pipe communicating port and an oil phase material branch pipe communicating port are sequentially arranged on the biological material flowing main pipeline along a biological material flowing path; one end of the cryopreservation agent branch pipeline is communicated with the biological material main circulation pipeline through a cryopreservation agent branch pipeline communication port, and the other end of the cryopreservation agent branch pipeline is a cryopreservation agent inlet; one end of the oil phase material branch pipeline is communicated with the biological material main circulation pipeline through an oil phase material branch pipeline communication port, and the other end of the oil phase material branch pipeline is an oil phase material inlet; the biological material inlet, the biological material oil drop outlet, the freezing preservative inlet and the oil phase material inlet are formed in the surface of the chip;
the freezing carrying rod is provided with a carrying groove for containing biological material oil drops, and the carrying groove is detachably communicated with a biological material oil drop outlet of the water-in-oil drop forming chip.
According to the device for freezing and storing the biological material, the water-in-oil droplet forming chip can realize automatic formation of adding the freezing and storing agent and generating the water-in-oil droplets by means of a microfluidic technology, manual operation is reduced, and efficiency is improved.
According to a specific embodiment of the present invention, in the apparatus for cryopreservation of a biological material of the present invention, the main flow pipeline of a biological material of the water-in-oil droplet formation chip may be further provided with one or more (e.g., 2, 3, 4, or 5) spare branch pipeline communication ports, which may be disposed upstream or downstream of the cryopreservative branch pipeline communication port.
According to a specific embodiment of the present invention, in the cryopreservation apparatus for biological materials of the present invention, in the water-in-oil droplet-forming chip, the one or more auxiliary branch channel communication ports may be provided upstream of the oil-phase material branch channel communication port.
According to a specific embodiment of the present invention, in the cryopreservation apparatus for biological materials of the present invention, the whole of the water-in-oil droplet formation chip is a Polydimethylsiloxane (PDMS) plate-shaped microfluidic chip.
According to a specific embodiment of the present invention, in the cryopreservation apparatus for biological materials of the present invention, the water-in-oil droplet forming chip has a length of 20 to 40mm, a width of 10 to 20mm, and a thickness of 4 to 10 mm.
According to a specific embodiment of the utility model, in the device for cryopreservation of a biological material, in the water-in-oil droplet formation chip, the sizes of a biological material inlet and a biological material oil droplet outlet of the main biological material flow pipeline are respectively and independently 1.5-2.5mm in caliber and 2.5-3.5mm in depth; the size of the inlet of each branch pipeline is 1.5-2.5mm in caliber and 2.5-3.5mm in depth respectively and independently. The biological material inlet, the biological material oil drop outlet and the inlet of each branch pipeline of the biological material main circulation pipeline can be the same or different in size.
According to a specific embodiment of the present invention, in the cryopreservation apparatus for biological materials of the present invention, in the water-in-oil droplet formation chip, the main flow channel and each branch channel of the biological materials have a width of 0.4 to 0.6mm and a depth of 1.5 to 2.5mm (i.e., each channel has a rectangular cross section). The sizes of the main biomaterial flowing pipeline and the branch pipelines can be the same or different.
According to a specific embodiment of the present invention, in the cryopreservation apparatus for biological materials of the present invention, in the water-in-oil droplet formation chip, the length of the main flow pipe of biological materials is 15 to 25mm, the length of the cryopreservation agent branch pipe is 3 to 8mm, and the length of the oil phase material branch pipe is 3 to 8 mm. The lengths of the branch ducts may be the same or different. In the present invention, the length of each of the conduits does not include the orifice depth.
According to a specific embodiment of the present invention, in the cryopreservation apparatus for a biological material according to the present invention, in the water-in-oil droplet formation chip, a flow direction distance between the cryopreservation agent branch conduit communication port and the biological material inlet is 3 to 5mm, a flow direction distance between the oil phase material branch conduit communication port and the biological material droplet outlet is 3 to 5mm, and a flow direction distance between the cryopreservation agent branch conduit communication port and the oil phase material branch conduit communication port is 3 to 15 mm. In the utility model, the flow direction distance between the freezing preservative branch pipeline communicating port and the biological material inlet, and the flow direction distance between the oil phase material branch pipeline communicating port and the biological material oil drop outlet do not comprise the depth of the pipe orifice.
According to a specific embodiment of the present invention, in the cryopreservation apparatus for biological materials of the present invention, in the water-in-oil droplet formation chip, each of the branch pipes is preferably disposed perpendicular to the main pipe.
According to the embodiment of the utility model, the water-in-oil droplet forming chip comprises four branch pipelines, and the branch pipelines and the communication port of the main pipeline are uniformly distributed along the flow direction of the main pipeline.
According to a specific embodiment of the present invention, in the cryopreservation apparatus for biological materials of the present invention, the water-in-oil droplets form a chip, and the overall size is 30mm in length, 15mm in width, and 5mm in thickness; the sizes of the biological material inlet, the biological material oil drop outlet and the inlets of the branch pipelines of the biological material main circulation pipeline are respectively 2mm in caliber and 3mm in depth; the width and the depth of each pipeline are respectively 0.5mm and 2 mm; the main pipeline length is 20mm, and each branch pipeline length is 5 mm. Preferably, the chip comprises four branch pipelines, and the distance (flowing direction along the main pipeline) between each branch pipeline and the adjacent communication port of the main pipeline is 4 mm.
In the freezing preservation device for the biological material, the object carrying groove of the freezing carrier rod is detachably communicated with the biological material oil drop outlet of the water-in-oil drop forming chip, and the object carrying groove of the freezing carrier rod and the biological material oil drop outlet of the water-in-oil drop forming chip are capable of separating the freezing carrier rod from the water-in-oil drop forming chip so as to conveniently carry out freezing preservation on the freezing carrier rod carrying the biological material oil drops.
In some embodiments of the utility model, the carrier groove on the freezing carrier rod is communicated with the biomaterial oil drop outlet of the water-in-oil drop forming chip through a flexible conduit. The flexible conduit is used for leading the biological material oil drops out of the water-in-oil drop forming chip and then leading the biological material oil drops into the carrying groove of the freezing carrying rod. The flexible conduit is detachable.
According to some embodiments of the present invention, in the biomaterial cryopreservation device according to the present invention, the opening of the loading groove on the freezing loading bar is circular or elliptical, the bottom of the groove is the biomaterial storage bin, the biomaterial storage bin has a circular or elliptical upper opening, and the opening area of the biomaterial storage bin is smaller than the opening area of the groove, an inclined groove side wall is formed from the opening of the groove to the upper opening of the biomaterial storage bin, the inclination of the groove side wall is 50 to 70 degrees, the length of the groove side wall in the inclined direction is 3 to 7mm, and the vertical depth from the groove opening to the bottom of the biomaterial storage bin is 3 to 7 mm.
According to some embodiments of the present invention, in the biomaterial cryopreservation device of the present invention, when the freezing loading rod is in use, the biomaterial oil droplets slide down along the side walls of the grooves to the biomaterial storage bin at the bottom, and the biomaterial oil droplets are covered with the inert oil for sealing, so that the biomaterial can be cryopreserved by liquid nitrogen. Wherein, the biomaterial storage bin can also be pre-filled with partial biomaterial protecting liquid or inert oil.
According to some embodiments of the utility model, the diameter of the opening of the loading groove of the freezing loading rod is 2-8mm, and the diameter of the opening of the biological material storage bin is 0.5-3 mm.
According to some embodiments of the present invention, when the opening of the loading groove of the freezing loading bar or the opening of the biological material storage bin is an ellipse, the diameter refers to the major axis and the minor axis of the ellipse, i.e. the major axis and the minor axis of the ellipse are both in the diameter size range.
According to some embodiments of the utility model, the biomaterial storage bin of the freezing loading rod is a spherical or semi-spherical cavity with a diameter of 1-3 mm.
According to some embodiments of the present invention, in the biomaterial cryopreservation apparatus according to the present invention, the biomaterial storage silo opening area of the freezing support rod is smaller than the maximum cross-sectional area of the biomaterial storage silo. The design is beneficial to better cover inert oil on the biomaterial oil drops in the biomaterial storage bin for sealing.
According to some embodiments of the present invention, the connection between the inclined groove sidewall of the freezing loading rod and the upper opening of the biomaterial storage bin is a rounded chamfer design, and the radius of the chamfer is less than or equal to 2mm, for example, 0.05-2 mm.
According to some embodiments of the present invention, in the biomaterial cryopreservation device according to the present invention, the opening of the loading groove of the freezing loading bar is circular with a diameter of 6mm, the slope of the side wall of the groove is 60 degrees, and the vertical depth from the opening of the groove to the bottom of the biomaterial storage bin is about 5 mm. The biomaterial storage bin is a hemispherical cavity with the diameter of 3 mm.
According to some embodiments of the utility model, in the cryopreservation device for biological materials, the inner wall of the carrying groove of the freezing carrying rod is made of PPO, F-4 polytetrafluoroethylene or P1 polyimide, which is beneficial to the preservation of biological material oil drops.
According to some embodiments of the present invention, in the cryopreservation apparatus for biological materials of the present invention, the freezing support rod of the freezing support rod is a freezing support rod made of PPO polyphenylene oxide, F-4 polytetrafluoroethylene or P1 polyimide.
According to some embodiments of the present invention, in the cryopreservation apparatus for biological materials of the present invention, the freezing support rod is a cylinder having a length of 10 to 20cm and a diameter of 5 to 25 mm; the carrying groove is arranged at the position 1-3cm away from the front end of the head of the freezing carrying rod.
According to some embodiments of the present invention, in the cryopreservation apparatus for biological materials of the present invention, the tail of the freezing carrying rod is a handheld end, and a biological material sample information bar code region is arranged on the tail of the freezing carrying rod.
In the present invention, terms used have substantially the same meaning as the related art unless otherwise noted.
For example, in the present invention, the "head" and "tail" of the freezing bar are described as conventional in the art, and the "tail" refers to the hand-held end and the other end is the head (or front end).
For example, in the present invention, the term "bottom" refers to the position of the groove opening upward.
For example, in the present disclosure, the term "spherical" means substantially spherical, and may include segments having a height above the full sphere height 3/4, for example; "hemispherical" means substantially hemispherical, and may include segments having a height of 3/4-1/4 of a full sphere, for example.
For example, in the present invention, the "inclination of the side wall of the groove" refers to an angle between the inclination direction of the side wall and the horizontal plane when the opening of the groove is upward.
According to some embodiments of the present invention, in the cryopreservation apparatus for biological material of the present invention, the front end of the head of the freezing loading rod is provided with a concave hole as a loading groove, and the opening end of the concave hole is detachably sleeved on the biological material oil drop outlet of the water-in-oil drop forming chip. The biomaterial oil drops directly enter the loading groove of the freezing loading rod after flowing out of the biomaterial oil drop outlet.
According to the specific embodiment of the utility model, after biological material oil drops are placed in the carrying groove on the freezing carrying rod, the biological material oil drops can be covered with an oil phase material (inert oil) for sealing, and then the biological material oil drops are subjected to liquid nitrogen freezing storage.
In conclusion, the utility model provides a closed type freezing device based on a water-in-oil technology, which can greatly improve the sealing property, realize the real 'closing', and avoid the embryo pollution risk caused by contacting liquid nitrogen; and the storage position of the biological material is relatively fixed, the biological material has high preservation firmness, and the possibility that the biological material is ejected or knocked down due to the inclination or the vibration of the carrying rod does not exist basically. In addition, the utility model can realize the automation of adding the cryopreservation agent and generating the water-in-oil droplets by means of the microfluidic technology, reduce manual operation, improve efficiency and avoid the possibility of embryo confusion and error caused by multiple steps in the traditional embryo freezing technology.
Drawings
FIG. 1 is a schematic view showing the structure of an apparatus for cryopreservation of biological materials in one embodiment of the present invention.
Fig. 2 is a schematic cross-sectional view of the loading groove of the freezing loading bar of the cryopreservation apparatus for biological materials according to an embodiment of the utility model.
FIG. 3 is a schematic view showing the structure of an apparatus for cryopreservation of biological materials in another embodiment of the present invention.
Detailed Description
For a more clear understanding of the technical features, objects and advantages of the present invention, reference is now made to the following detailed description taken in conjunction with the accompanying specific embodiments, and the technical solutions of the present invention are described, it being understood that these examples are intended to illustrate the present invention and are not intended to limit the scope of the present invention.
Example 1
As shown in FIG. 1, the present embodiment provides an apparatus for cryopreservation of a biological material, which comprises a water-in-oil droplet formation chip 10 and a freezing bar 20.
The water-in-oil droplet forming chip 10 includes a main circulation pipeline of biological material, a branch pipeline of cryopreservation agent, and a branch pipeline of oil phase material: one end of the main biomaterial flowing pipeline is a biomaterial inlet 101, and the other end of the main biomaterial flowing pipeline is a biomaterial oil drop outlet 102; a cryopreservation agent branch pipeline communicating port and an oil phase material branch pipeline communicating port are arranged on the biological material main flow pipeline in sequence along a biological material flow path; one end of the cryopreservation agent branch pipeline is communicated with the main biological material flowing pipeline through a cryopreservation agent branch pipeline communication port, and the other end of the cryopreservation agent branch pipeline is provided with a cryopreservation agent inlet 104; one end of the oil phase material branch pipeline is communicated with the biological material main circulation pipeline through an oil phase material branch pipeline communication port, and the other end of the oil phase material branch pipeline is an oil phase material inlet 103. The main circulation pipeline of the biological material of the water-in-oil droplet forming chip 10 is also provided with a plurality of spare branch pipelines, 2 spare branch pipelines are shown in figure 1, the surfaces of the chips are respectively provided with material inlets 105 and 106, the spare branch pipelines are arranged at the downstream of the cryopreservation agent branch pipeline and the upstream of the oil phase material branch pipeline, and are used for standby or used as liquid inlet of other reagents required by embryo freezing, such as cane sugar and the like, and can be selectively used or not used according to actual needs. The water-in-oil droplet forming chip 10 is a PDMS plate-like microfluidic chip as a whole, the main pipeline and each branch pipeline are arranged inside the chip, and the inlet of each pipeline and the outlet of the main pipeline are arranged on the surface of the chip (preferably on the same side surface of the chip). All reagents can be injected manually by syringe or automatically by electronic pumping means.
In this embodiment, the water-in-oil droplet forming chip 10 has an overall size of 30mm in length, 15mm in width and 5mm in thickness; the sizes of the biological material inlet, the biological material oil drop outlet and the inlets of the branch pipelines of the biological material main circulation pipeline are respectively 2mm and 3 mm; the width and the depth of each pipeline are respectively 0.5mm and 2 mm; the main pipeline length is 20mm, and each branch pipeline length is 5 mm. The distance between each branch pipeline and the adjacent communicating port of the main pipeline is 4 mm.
The whole freezing carrier rod 20 is made of PPO polyphenylene oxide. The head of the freezing carrier rod 20 is provided with a carrier groove 201 for accommodating the biological material (the opening of the carrier groove 201 is substantially circular). The tail part of the freezing carrying rod 20 is a handheld end and is provided with a biological material sample information bar code area 202.
In this embodiment, the whole freezing carrying rod 20 is a cylinder with a length of 15cm and a diameter of 25 mm; the loading groove 201 is arranged at the position 2cm away from the front end of the head of the freezing loading rod, and the biological material sample information bar code area 202 is arranged at the position 2cm away from the tail end.
Referring to fig. 2 again, in the present embodiment, the opening of the loading recess 201 is circular with a diameter of 6mm, the bottom of the recess is a biomaterial storage silo 2011, the biomaterial storage silo 2011 is a multi-hemispherical cavity with a diameter of 3mm, and has a circular upper opening, an inclined recess sidewall 2012 with a tapered opening is formed between the opening of the recess and the upper opening of the biomaterial storage silo, the inclined slope of the recess sidewall 2012 is 60 degrees, and the vertical depth from the recess opening to the bottom of the biomaterial storage silo is about 5 mm. The connection of the inclined groove side walls to the upper opening of the biomaterial storage silo (as indicated by the arrow in fig. 2) may be designed with a rounded chamfer, which may have a radius of 0.1mm, for example.
In a specific application, the cryoprotectant can be pre-loaded into the chip 10 through the inlet 104, the inlet 105 and/or the inlet 106; injecting a cryoprotectant oil into the chip through inlet 103; the biological material to be preserved is injected from the inlet 101. The water-in-oil droplets that have the biomaterial encapsulated therein are collected through the biomaterial oil droplet outlet 102. After the water-in-oil droplets flow out from the biomaterial droplet outlet 102, the water-in-oil droplets can be guided into the carrying groove 201 of the embryo freezing carrying rod 20 through the transparent flexible conduit 30 for liquid nitrogen freezing preservation.
Example 2
This example provides another device for cryopreservation of embryos, such as day 5-6 blastocysts, which is shown in FIG. 3, and comprises a water-in-oil droplet formation chip 10 and a freezing carrier rod 30. The structure of the water-in-oil droplet forming chip 10 is substantially the same as that of example 1. The front end of the head of the freezing carrying rod 30 is provided with a concave hole as a carrying groove 301, and the opening end of the carrying groove 301 is detachably sleeved on the biomaterial oil drop outlet 102 of the water-in-oil drop forming chip. The tail of the freezing carrying rod 30 is a handheld end and is provided with a biological material sample information bar code area 302.
The biomaterial oil drop A formed by the water-in-oil drop forming chip 10 directly enters the loading groove 301 of the freezing loading rod 30 after flowing out of the biomaterial oil drop outlet 102, and then the biomaterial oil drop A is covered with an oil phase material (inert oil) B for sealing. Then, the freezing bar 30 is detached from the water-in-oil droplet forming chip 10, and the freezing bar 30 carrying the biomaterial droplets A is subjected to liquid nitrogen cryopreservation.
Claims (10)
1. A cryopreservation apparatus for biological materials, comprising a water-in-oil droplet formation chip and a freezing carrier rod, wherein:
the water-in-oil droplet forming chip comprises a main biological material flowing pipeline, a cryopreservation agent branch pipeline and an oil phase material branch pipeline which are arranged in the chip; wherein: one end of the biological material main flow pipeline is a biological material inlet, and the other end of the biological material main flow pipeline is a biological material oil drop outlet; a cryopreservation agent branch pipeline communicating port and an oil phase material branch pipeline communicating port are arranged on the biological material main flow pipeline in sequence along a biological material flow path; one end of the cryopreservation agent branch pipeline is communicated with the biological material main circulation pipeline through a cryopreservation agent branch pipeline communication port, and the other end of the cryopreservation agent branch pipeline is a cryopreservation agent inlet; one end of the oil phase material branch pipeline is communicated with the biological material main circulation pipeline through an oil phase material branch pipeline communication port, and the other end of the oil phase material branch pipeline is an oil phase material inlet; the biological material inlet, the biological material oil drop outlet, the freezing preservative inlet and the oil phase material inlet are formed in the surface of the chip;
the freezing carrying rod is provided with a carrying groove for accommodating biological material oil drops, and the carrying groove is detachably communicated with a biological material oil drop outlet of the water-in-oil drop forming chip.
2. The cryopreservation device for biological materials as claimed in claim 1, wherein the water-in-oil droplet forming chip is a polydimethylsiloxane plate microfluidic chip as a whole.
3. The cryopreservation apparatus of biological material as claimed in claim 1, wherein the water-in-oil droplet forming chips have a length of 20-40mm, a width of 10-20mm and a thickness of 4-10 mm;
the sizes of the biological material inlet and the biological material oil drop outlet of the biological material main circulation pipeline are respectively and independently 1.5-2.5mm in caliber and 2.5-3.5mm in depth; the size of the inlet of each branch pipeline is respectively and independently 1.5-2.5mm in caliber and 2.5-3.5mm in depth; the biological material inlet, the biological material oil drop outlet and the inlets of the branch pipelines of the biological material main circulation pipeline are the same or different in size;
the sizes of the main circulation pipeline and each branch pipeline of the biological material are respectively and independently 0.4-0.6mm in width and 1.5-2.5mm in depth; the sizes of the main biological material flowing pipeline and the branch pipelines are the same or different;
the length of the main biological material flowing pipeline is 15-25mm, the length of the cryopreservation agent branch pipeline is 3-8mm, and the length of the oil phase material branch pipeline is 3-8 mm;
the flow direction distance between the cryopreservation agent branch pipeline communicating port and the biological material inlet is 3-5mm, the flow direction distance between the oil phase material branch pipeline communicating port and the biological material oil drop outlet is 3-5mm, and the flow direction distance between the cryopreservation agent branch pipeline communicating port and the oil phase material branch pipeline communicating port is 3-15 mm.
4. The cryopreservation apparatus for biological materials as claimed in claim 1, wherein the overall size of the water-in-oil droplet formation chip is 30mm long, 15mm wide and 5mm thick; the sizes of the biological material inlet, the biological material oil drop outlet and the inlets of the branch pipelines of the biological material main circulation pipeline are respectively 2mm in caliber and 3mm in depth; the width and the depth of each pipeline are respectively 0.5mm and 2 mm; the main pipeline length is 20mm, and each branch pipeline length is 5 mm.
5. The cryopreservation device for biological materials as claimed in claim 1, wherein the carrying grooves on the freezing carrying rod are communicated with the biological material oil drop outlet of the water-in-oil drop forming chip through a flexible conduit.
6. The apparatus for cryopreservation of a biological material according to claim 1 or 5, wherein the opening of the loading groove on the freezing loading bar is circular or elliptical, the bottom of the groove is a biological material storage bin, the biological material storage bin has a circular or elliptical upper opening, and the opening area of the biological material storage bin is smaller than the opening area of the groove, an inclined groove side wall is formed from the opening of the groove to the upper opening of the biological material storage bin, the inclination of the groove side wall is 50 to 70 degrees, the length of the groove side wall in the inclined direction is 3 to 7mm, and the vertical depth from the groove opening to the bottom of the biological material storage bin is 3 to 7 mm;
the opening diameter of the carrying groove is 2-8mm, and the opening diameter of the biological material storage bin is 0.5-3 mm;
the biomaterial storage bin is a spherical cavity or a hemispherical cavity with the diameter of 1-3 mm.
7. The apparatus for cryopreservation of biological materials as claimed in claim 1 or 5, wherein the opening of the loading groove is circular with a diameter of 6mm, the slope of the side wall of the groove is 60 degrees, and the vertical depth from the opening of the groove to the bottom of the biological material storage bin is 5 mm; the biomaterial storage bin is a hemispherical cavity with the diameter of 3 mm.
8. The apparatus for cryopreservation of biological materials as claimed in claim 1 or 5, wherein the freezing bar is a polyphenylene ether freezing bar, a polytetrafluoroethylene freezing bar, or a polyimide freezing bar.
9. The cryopreservation apparatus for biological materials as claimed in claim 1 or 5, wherein the whole freezing carrying rod is a cylinder with a length of 10-20cm and a diameter of 5-25 mm;
the carrying groove is arranged at the position 1-3cm away from the front end of the head of the freezing carrying rod;
the tail part of the freezing carrying rod is a handheld end and is provided with a biological material sample information bar code area.
10. The cryopreservation apparatus for biological materials as claimed in claim 1, wherein the front end of the head of the freezing carrying rod is provided with a concave hole as a carrying groove, and the opening end of the concave hole is detachably sleeved on the biological material oil drop outlet of the water-in-oil drop forming chip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123364524.3U CN216931621U (en) | 2021-12-29 | 2021-12-29 | Freezing save set of biomaterial |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123364524.3U CN216931621U (en) | 2021-12-29 | 2021-12-29 | Freezing save set of biomaterial |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216931621U true CN216931621U (en) | 2022-07-12 |
Family
ID=82311799
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202123364524.3U Active CN216931621U (en) | 2021-12-29 | 2021-12-29 | Freezing save set of biomaterial |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216931621U (en) |
-
2021
- 2021-12-29 CN CN202123364524.3U patent/CN216931621U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Hafez | Preservation and cryopreservation of gametes and embryos | |
| JP5278978B2 (en) | Tubular for vitrification preservation of animal embryos or eggs | |
| US20140308655A1 (en) | Device for Manipulating Biological Materials in a Process of Cryopreservation and a Use of Such a Device | |
| WO2010056755A4 (en) | Microfluidic embryo and gamete culture systems | |
| CN103179852A (en) | Improved micromanipulation and storage apparatus and methods | |
| JP6475493B2 (en) | Germ cell preservation tool and germ cell preservation method | |
| US5495719A (en) | Method of preserving spermatozoa | |
| CN102160546B (en) | The system and method for Cell Cryopreservation | |
| JP2009148221A (en) | Freeze preservation vessel for reproductive cell and method for freeze preservation of reproductive cell | |
| CN216931621U (en) | Freezing save set of biomaterial | |
| US10512261B2 (en) | Containers for liquid nitrogen storage of semen straws | |
| JP4431754B2 (en) | Vitrification preservation tool of embryo and vitrification preservation method of embryo using the same | |
| CN216701452U (en) | Chip for forming water-in-oil droplets for cryopreservation of biological materials | |
| CN114223650A (en) | Cryopreservation method and device for biological material | |
| JP2006149231A (en) | Narrow tube for freeze preservation of biological specimen, freeze preservation method for biological specimen and method for melting after freeze preservation | |
| US20080269549A1 (en) | Method, article, and apparatus for cryopreservation of biological samples | |
| JP2014184056A (en) | Mammalian embryo storing device and mammalian embryo implantation straw, and application thereof | |
| CN114762498B (en) | Biological material processing device | |
| JP2016067807A (en) | Straw tube, and cryopreservation method of semen or fertilized egg | |
| CN215075025U (en) | Single sperm freezing carrier | |
| CN212393695U (en) | Sperm freezing carrier system | |
| JP6734524B2 (en) | Straws for preservation, thawing, dilution, and transplantation of vitrified mammalian embryos and methods of using the same | |
| WO2010049774A2 (en) | Inspection block for use in microscopic inspection of embryos or other biological material inside a container unit | |
| Jones | Cryopreservation of bovine embryos | |
| CN215224290U (en) | Multi-functional cell cryopreserving box |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |