CN215224290U

CN215224290U - Multi-functional cell cryopreserving box

Info

Publication number: CN215224290U
Application number: CN202121455642.2U
Authority: CN
Inventors: 胡传圣; 沈益行; 张蕊
Original assignee: Suzhou Jingmai Biotechnology Co ltd
Current assignee: Suzhou Jingmai Biotechnology Co ltd
Priority date: 2021-06-29
Filing date: 2021-06-29
Publication date: 2021-12-21
Anticipated expiration: 2031-06-29

Abstract

The utility model provides a multi-functional cell cryopreserving box, including insulation can and freezer, the insulation can includes insulation can and lower insulation can, the freezer sets up in insulation can down, be provided with centrifugal device between freezer and insulation can, it is provided with the rubber gasket unanimous with through-hole size and quantity to go up on the insulation can, be provided with two spring leafs in the through-hole, place the cryopreserving pipe between the spring leaf, the other label shell that is provided with of through-hole, insulation can's right side middle part is provided with the glass pipe down, be provided with the scale mark on the glass pipe, the inside of glass pipe and insulation can is linked together down, insulation can's right side bottom is provided with the drain pipe down, be provided with the drain valve on the drain pipe, insulation can box body left side is provided with the feed liquor pipe down, be provided with the feed liquor valve on the feed liquor pipe, drain pipe and feed liquor pipe are connected with the freezer. Wherein the utility model has the advantages that: the freezing and storing pipes with different sizes can be stored, and the freezing and storing operation and the storing can be carried out simultaneously.

Description

Multi-functional cell cryopreserving box

Technical Field

The utility model relates to a biological equipment technical field, in particular to multi-functional cell cryopreserving box.

Background

There are two common ways of freezing cells: the traditional freezing storage mode is that the cells are placed at 4 ℃ for 30-60 minutes, transferred to-20 ℃ for 30-60 minutes, then transferred to-80 ℃ for 16-18 hours, and finally placed in liquid nitrogen for long-term storage, and the traditional freezing storage mode has multiple steps, long time consumption, difficult control of the time of placing the cells at 4 ℃ and-20 ℃, non-uniform cooling rate, relatively large damage to the cells and low survival rate after recovery; the other type is a program cooling box sold in the market, cells added with freezing liquid are placed in the box, the cells are directly placed in liquid nitrogen for long-term storage at minus 80 ℃ overnight, good slow cooling rate can be provided, isopropanol is required to be added into the program cooling box, the isopropanol is volatile, in order to play a good program cooling effect, the amount of the isopropanol is required to be guaranteed and cannot be lower than a certain amount, the existing program cooling box generally adopts a low-temperature-resistant PE material, the transparency is poor, the liquid level height in the cooling box is not easy to observe, the liquid level height needs to be checked by opening a box cover, so that cold air in the freezing box is quickly lost to cause cell thawing, the survival rate of the recovered cells is influenced, in addition, the existing program cooling box is single in function, only freezing tubes with a single structure can be placed, and freezing tubes with different heights cannot be stored.

SUMMERY OF THE UTILITY MODEL

In order to solve the technical problem, the utility model discloses a hold not unidimensional cell cryopreserving box that can carry out cell cryopreserving, the technical scheme of the utility model is implemented like this:

a multifunctional cell cryopreservation box comprises an incubator and a freezing box, wherein the incubator comprises an upper incubator body and a lower incubator body, the freezing box is arranged in the lower incubator body, a centrifugal device is arranged between the freezing box and the incubator body, the bottom of the lower incubator body is provided with a TypeC interface, the TypeC interface is connected with the centrifugal device, one end of the upper incubator body is connected with the lower incubator body through a hinge, 9 through holes are uniformly arranged in the freezing box, a sealing rubber ring with the size and the number consistent with those of the through holes is arranged on the upper incubator body, two spring pieces are arranged in the through holes, the spring pieces are symmetrically arranged along the through holes in an axial line manner, a cryopreservation tube is arranged between the spring pieces, a label shell is arranged beside the through holes, and a glass tube is arranged in the middle part of the right side of the lower incubator body, the refrigerator is characterized in that scale marks are arranged on the glass tube, the glass tube is communicated with the interior of the lower heat preservation box body, a liquid outlet tube is arranged at the bottom of the right side of the lower heat preservation box body, a liquid outlet valve is arranged on the liquid outlet tube, a liquid inlet tube is arranged on the left side of the box body of the lower heat preservation box body, a liquid inlet valve is arranged on the liquid inlet tube, and the liquid outlet tube and the liquid inlet tube are connected with the refrigerator.

Preferably, the left side and the right side of the freezing box are provided with valve interfaces, and the valve interfaces are respectively connected with the liquid inlet pipe and the liquid outlet pipe.

Preferably, a connecting valve is arranged on the valve interface.

Preferably, a handle is arranged in the middle of the top of the upper heat preservation box body.

Preferably, the upper heat preservation box body is provided with an upper buckle, the lower heat preservation box body is provided with a lower buckle, and the upper heat preservation box body and the lower heat preservation box body are connected through the upper buckle and the lower buckle.

Preferably, a sealing ring is arranged on the upper heat preservation box body, and the size of the sealing ring is consistent with the size of the space between the lower heat preservation box body and the freezing box.

Preferably, a protective cover is arranged on the TypeC interface.

By implementing the technical scheme of the utility model, the problems that the freezing storage box can only store freezing storage pipes with certain sizes and the storage and freezing storage operations need to be separated in the prior art can be solved; implement the technical scheme of the utility model, through setting up centrifuge and feed liquor pipe and drain pipe between spring leaf and insulation can and the freezer, can realize depositing not unidimensional cryopreserved pipe to and can realize freezing the operation of depositing and preserve the technological effect of going on with the machine.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only one embodiment of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.

In which like parts are designated by like reference numerals. It should be noted that the terms "front," "back," "left," "right," "upper" and "lower" used in the following description refer to directions in the drawings, and the terms "bottom" and "top," "inner" and "outer" refer to directions toward and away from, respectively, the geometric center of a particular component.

FIG. 1 is a schematic diagram of a cell cryopreservation box structure;

FIG. 2 is a schematic diagram of a rear view structure of the cell cryopreservation box.

In the above drawings, the reference numerals denote:

1 Upper insulation box

2 lower thermal insulation box body

3 freezing box

4 liquid inlet pipe

5 sealing rubber ring

6 go up buckle

7 sealing ring

8 liquid outlet pipe

9 glass tube

10 lower buckle

11 through hole

12 hinge

13type C interface

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

Examples

In a preferred embodiment, as shown in fig. 1, a multifunctional cell cryopreservation box comprises an incubator and a freezing box, wherein the incubator comprises an upper incubator body 1 and a lower incubator body 2, the freezing box 3 is arranged in the lower incubator body 2, a centrifugal device is arranged between the freezing box 3 and the lower incubator body 2, a TypeC interface 13 is arranged at the bottom of the lower incubator body 2, the TypeC interface 13 is connected with the centrifugal device, one end of the upper incubator body 1 is connected with the lower incubator body 2 through a hinge 12, 9 x 9 through holes 11 are uniformly arranged in the freezing box 3, sealing rubber rings 5 with the size and the number consistent with those of the through holes 11 are arranged on the upper incubator body 1, two spring pieces are arranged in the through holes, the spring pieces are axially and symmetrically arranged along the through holes, a cryopreservation tube is arranged between the spring pieces, a label shell is arranged beside the through holes 11, a glass tube 9 is arranged in the middle part of the right side of the lower incubator body 2, be provided with the scale mark on the glass pipe 9, the inside of glass pipe 9 and heat preservation box 2 is linked together down, and the right side bottom of heat preservation box 2 is provided with drain pipe 8 down, is provided with the drain valve on the

drain pipe

8, and 2 left sides of heat preservation box are provided with feed liquor pipe 4 down, are provided with the feed liquor valve on the feed liquor pipe 4, and drain pipe 8 and feed liquor pipe 4 are connected with freezer 3.

In this specific embodiment, adopt insulation can and freezer 3 two parts, the insulation can includes insulation can 1 and lower insulation can 2 two parts, wherein, freezer 3 sets up in insulation can 2 down, the one end of upper insulation can 1 and lower insulation can 2 is connected through loose-leaf 12, the other end of lower insulation can 1 and upper insulation can 2 is connected fixedly through the buckle, set up buckle 6 on the upper insulation can 1, set up buckle 10 down on the lower insulation can 2, connect through buckle 6 and buckle 10 down and can effectually guarantee to go up the connection between insulation can 1 and the lower insulation can 2, prevent to take place the problem that the sample in the freezing box scatters in the middle of the in-process of transportation, be provided with the handle in the top intermediate position of upper insulation can 1, can conveniently transport the cell freezing box. Freezer 3 sets up in heat preservation box 2 down, freezer 3 is connected fixedly through centrifugal device with heat preservation box 2 down, be provided with feed liquor pipe 4 and drain pipe 8 under on heat preservation box 2, feed liquor pipe 4 and drain pipe 8 set up the left and right sides at heat preservation box 2 down respectively, feed liquor pipe 4 can be to the interior isopropyl alcohol of input of freezer 3, be provided with the glass pipe 9 that is carved with scale under simultaneously on heat preservation box 2, because isopropyl alcohol volatilizees easily, through setting up the content of the observation isopropyl alcohol that has scale glass pipe 9 and can understand, can supply through feed liquor pipe 4 simultaneously, perhaps discharge unnecessary liquid through fluid-discharge tube 8. At the centrifugal device that sets up between freezer 3 and the insulation can body 2 down, set up type C interface 13 on insulation can body 2 down, type C interface 13 is connected with centrifugal device, can provide power to centrifugal device, because type C interface 13 has fine compatibility, consequently chooses for use type C interface 13, still is provided with the safety cover on type C interface 13, and the safety cover can effectually protect type C interface 13 to prevent to be infected with liquid or suffer wearing and tearing etc.. Be provided with 9 x 9 through-holes 11 in freezer 3, set up two spring leafs in the through-hole 11 and realized can both preserving and transporting to the cryopreserved pipe of unidimensional, set up in last heat preservation box body 1 and through-hole 11 size unanimous sealed rubber ring 5 can guarantee to fix the cryopreserved pipe of depositing in through-hole 11 in, prevent to freeze the transportation of depositing the box and deposit the pipe and bump, can play certain sealed effect simultaneously. Still be provided with the label box beside through-hole 11, can make things convenient for operating personnel to carry out the efficiency that corresponding operation improved the experiment according to the suggestion. The freezer is connected with feed liquor pipe 4 and drain pipe 8 respectively, is provided with the valve interface on freezer 3, and the valve interface is connected with feed liquor pipe and drain pipe respectively, sets up the connecting valve on the valve interface, and the connecting valve can control the connection of feed liquor pipe 4 and drain pipe 8 and freezer 3, and when the disconnection, can guarantee the leakproofness of freezer 3 simultaneously. Prevent to be equipped with the cryopreserved pipe of cell in through-hole 11, add trypsin to cryopreserved intraductal, with type C interface 13 on being connected to the power, start centrifugal device, realize the cryopreserved operation to the cell.

In a preferred embodiment, the left and right sides of the freezer compartment 3 are provided with valve ports, which are connected to the liquid inlet pipe 4 and the liquid outlet pipe 8, respectively.

In this particular embodiment, the freezer compartment 3 is connected to an inlet conduit 4 and an outlet conduit 8 to enable timely replenishment of the freezer compartment 3 and removal of associated liquid.

In a preferred embodiment, a connecting valve is arranged on the valve interface.

In this specific embodiment, set up the connecting valve on the valve interface, the connecting valve can control the connection of feed liquor pipe 4 and drain pipe 8 and freezer 3, when the disconnection, can guarantee the leakproofness of freezer 3 simultaneously.

In a preferred embodiment, a handle is arranged in the middle of the top of the upper heat preservation box body 1.

In this specific embodiment, a handle is arranged at the middle position of the top of the upper thermal insulation box body 1, so that the cell freezing box can be conveniently transported.

In a preferred embodiment, the upper heat preservation box body 1 is provided with an upper buckle 6, the lower heat preservation box body 2 is provided with a lower buckle 10, and the upper heat preservation box body 1 and the lower heat preservation box body 2 are connected through the upper buckle 6 and the lower buckle 10.

In this concrete embodiment, the other end of heat preservation box 2 and last heat preservation box 1 is connected fixedly through the buckle down, goes up to set up buckle 6 on the heat preservation box 1, sets up buckle 10 down on the heat preservation box 2 down, connects through last buckle 6 and buckle 10 down and can effectually guarantee to go up being connected between heat preservation box 1 and the heat preservation box 2 down, prevents to take place to freeze the problem that the sample in the case of depositing scatters in the middle of the process of transportation.

In a preferred embodiment, the upper thermal container 1 is provided with a gasket 7, the gasket 7 having a size corresponding to the size of the space between the lower thermal container 2 and the freezer compartment 3.

In this specific embodiment, the upper heat-insulating box body 1 is provided with the sealing ring 7, a certain gap exists between the freezer 3 and the lower heat-insulating box body 2, and the sealing ring 7 with the size consistent with that of the gap is arranged on the upper heat-insulating box body 1, so that air in the heat-insulating box can be effectively prevented from flowing, and the heat-insulating effect is improved.

In a preferred embodiment, a protective cover is provided on the TypeC interface 13.

In this specific embodiment, a protective cover is further disposed on the TypeC interface 13, and the protective cover can effectively protect the TypeC interface 13 from being contaminated with liquid or being worn and torn.

It should be understood that the above description is only exemplary of the present invention, and is not intended to limit the present invention, and that any modifications, equivalents, improvements, etc. made within the spirit and principle of the present invention should be included within the scope of the present invention.

Claims

1. The utility model provides a multi-functional cell cryopreserving box, includes insulation can and freezer, the insulation can includes insulation can and lower insulation can, the freezer sets up in the lower insulation can, its characterized in that: a centrifugal device is arranged between the freezing box and the heat preservation box body, a TypeC interface is arranged at the bottom of the lower heat preservation box body and is connected with the centrifugal device, one end of the upper heat preservation box body is connected with the lower heat preservation box body through a hinge, 9X 9 through holes are uniformly arranged in the freezing box body, a sealing rubber ring with the size and the number consistent with those of the through holes is arranged on the upper heat preservation box body, two spring pieces are arranged in the through holes and symmetrically arranged along the through holes in an axial line manner, a cryopreservation tube is arranged between the spring pieces, a label shell is arranged beside the through holes, a glass tube is arranged in the middle of the right side of the lower heat preservation box body, a scale mark is arranged on the glass tube, the glass tube is communicated with the inside of the lower heat preservation box body, a liquid outlet tube is arranged at the bottom of the right side of the lower heat preservation box body, and a liquid outlet valve is arranged on the liquid outlet tube, the left side of the box body of the lower heat preservation box body is provided with a liquid inlet pipe, a liquid inlet valve is arranged on the liquid inlet pipe, and the liquid outlet pipe and the liquid inlet pipe are connected with the freezing box.

2. The multifunctional cell cryopreservation box of claim 1, wherein: the left side and the right side of the freezing box are provided with valve interfaces which are respectively connected with the liquid inlet pipe and the liquid outlet pipe.

3. The multifunctional cell cryopreservation box of claim 2, wherein: and a connecting valve is arranged on the valve port.

4. A multifunctional cell cryopreservation kit as claimed in claim 3, wherein: and a handle is arranged in the middle of the top of the upper heat-insulating box body.

5. The multifunctional cell cryopreservation box of claim 4, wherein: the upper heat preservation box body is provided with an upper buckle, the lower heat preservation box body is provided with a lower buckle, and the upper heat preservation box body and the lower heat preservation box body are connected through the upper buckle and the lower buckle.

6. The multifunctional cell cryopreservation box of claim 5, wherein: and a sealing ring is arranged on the upper heat preservation box body, and the size of the sealing ring is consistent with the size of the space between the lower heat preservation box body and the freezing box.

7. The multifunctional cell cryopreservation box of claim 6, wherein: and a protective cover is arranged on the TypeC interface.