CN110754465A - Oocyte normal atmospheric temperature save set - Google Patents
Oocyte normal atmospheric temperature save set Download PDFInfo
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- CN110754465A CN110754465A CN201911155882.8A CN201911155882A CN110754465A CN 110754465 A CN110754465 A CN 110754465A CN 201911155882 A CN201911155882 A CN 201911155882A CN 110754465 A CN110754465 A CN 110754465A
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- oocyte
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
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Abstract
The invention discloses a normal-temperature oocyte preservation device, relates to the technical field of oocyte preservation, and solves the problem that in the prior art, an oocyte preservation device is easy to damage oocytes, and the technical key points are as follows: the oocyte preservation solution in the liquid storage tank is conveyed to the placing groove by the micro-flow pump through the circulating pipe and flows in the placing groove, an oocyte preservation tube is arranged in the placing groove, the surface of the oocyte preservation tube is also provided with a penetration hole, the oocyte preservation tubes are provided with a plurality of groups, and one oocyte is placed in a single oocyte preservation tube; the invention prevents the oocyte from being damaged in the preservation process.
Description
Technical Field
The invention relates to the technical field of oocyte preservation, in particular to a normal-temperature oocyte preservation device.
Background
In the prior art, cryopreservation is mainly adopted for oocyte preservation, and particularly, the oocyte is carried on a special freezing carrier for preservation. However, with the prior art, the requirements for devices for preserving oocytes are high, and the materials of these devices must be resistant to low temperatures and cannot affect oocytes, so that the material costs are high.
Chinese patent publication No. CN205106147U discloses a pig oocyte normal temperature preservation device, including: the device comprises a glass tube, a glass cover, a porcine oocyte storage plate and a light shield; the glass tube is hermetically connected with the glass cover; the pig oocyte storage plate is fixedly arranged in the glass tube, and a concave grid for storing pig oocytes is arranged on the pig oocyte storage plate; the light shield is sleeved outside the glass tube. The device has realized that pig oocyte can seal the save at normal atmospheric temperature, and this save set simple structure, and it is low to the material requirement, has practiced thrift the cost. However, in the case of this device, when the oocyte is preserved, the concentration of the preservation solution in the device changes with the lapse of the preservation time, and the oocyte is damaged, and therefore, an apparatus for preserving an oocyte at normal temperature has been proposed.
Disclosure of Invention
The invention aims to solve the technical problem and provides a normal-temperature oocyte preservation device, which comprises a box body, wherein the box body is divided into a lower box body and an upper box body, the lower box body is hermetically connected with the upper box body, a placing groove is arranged in the lower box body, the placing groove is connected with a micro-flow pump and a nutrient solution collecting tank through a circulating pipe, the micro-flow pump is communicated with a liquid storage box, oocyte preservation solution is stored in the liquid storage box, the micro-flow pump conveys the oocyte preservation solution in the liquid storage box to the placing groove through the circulating pipe and keeps the oocyte preservation solution in the placing groove to flow, an oocyte preservation pipe is arranged in the placing groove, the surface of the oocyte preservation pipe is also provided with a plurality of groups of permeation holes, and one oocyte is placed in each oocyte preservation pipe, the problem that the oocyte preservation device in the prior art has the risk of easily damaging oocytes is solved.
In order to achieve the purpose, the invention provides the following technical scheme:
the utility model provides an oocyte normal atmospheric temperature save set, comprises a box bod, the box body divide into box body and last box body down, box body and last box body sealing connection down, be equipped with the standing groove in the box body down, the standing groove is connected with microflow pump and nutrient solution collecting vat through the runner pipe, the microflow pump intercommunication has the stock solution box, the storage has oocyte preservative solution in the stock solution box, the oocyte preservative solution in the microflow pump will stock the liquid box is carried to the standing groove and is kept the standing groove in oocyte preservative solution flows through the runner pipe, be equipped with oocyte preservation pipe in the standing groove, the surface of oocyte preservation pipe still is equipped with the infiltration hole, oocyte preservation pipe is equipped with the multiunit, and place an oocyte in the single oocyte preservation pipe.
As a further scheme of the invention, a sealing ring is arranged between the lower box body and the upper box body, the air exhaust hole is communicated with the placing groove, and a one-way valve is arranged in a passage between the air exhaust hole and the placing groove.
As a further scheme of the invention, the lower box body is also provided with two accessory grooves, the two accessory grooves are respectively arranged at two sides of the placing groove, the liquid storage box, the micro-flow pump and the flow valve are arranged in one of the accessory grooves, and the liquid storage box is arranged in the other accessory groove.
As a further proposal of the invention, the diameter of the surface penetration hole of the oocyte preservation tube is less than 0.4 mm.
As a further proposal of the invention, a plurality of groups of liquid outlets are arranged in the placing groove of the circulating pipe; the circulation pipe between the nutrient solution collecting groove and the placing groove is provided with a plurality of groups of liquid inlets in the placing groove.
As a further proposal of the invention, the flow pipe is also provided with a flow valve, and the flow rate of the liquid in the flow pipe is 0.2-0.5 mm/s.
As a further scheme of the invention, the oocyte preservation tube is connected to the box body through a fixing ring, the fixing ring is a mesh tube with the same outer diameter as the oocyte preservation tube, and the fixing ring is inserted into the bottom of the placing groove.
As a further proposal of the invention, the opening part of the oocyte preservation tube is provided with a sealing plug.
In conclusion, compared with the prior art, the invention has the following beneficial effects:
according to the invention, the concentration of the oocyte preservation solution is reduced by slowly flowing and always supplementing a new oocyte preservation solution, so that the preservation environment of the oocyte is maintained, the change of the environment in the cell preservation tube and the concentration of the oocyte preservation solution is small, and the damage of the oocyte in the preservation process is prevented.
Drawings
FIG. 1 is a schematic structural diagram of an oocyte normal temperature preservation device.
FIG. 2 is a full sectional view of the oocyte cryopreservation apparatus.
FIG. 3 is a schematic structural diagram of a lower box body in the oocyte normal temperature preservation device.
Reference numerals: 1-box body, 11-lower box body, 12-upper box body, 13-air exhaust hole, 14-placement groove, 15-accessory groove, 2-oocyte preservation tube, 3-fixing ring, 4-flow tube, 5-liquid storage box, 6-microflow pump, 7-flow valve, 8-nutrient solution collecting tank and 9-one-way valve.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.
Example 1
As shown in fig. 1 to 2, a normal-temperature oocyte preservation device includes a box body 1, the box body 1 is divided into a lower box body 11 and an upper box body 12, the lower box body 11 is hermetically connected with the upper box body 12, a placement groove 14 is arranged in the lower box body 11, the placement groove 14 is connected with a micro-flow pump 6 and a nutrient solution collecting tank 8 through a circulation pipe 4, the micro-flow pump 6 is communicated with a liquid storage box 5, oocyte preservation liquid is stored in the liquid storage box 5, the micro-flow pump 6 conveys the oocyte preservation liquid in the liquid storage box 5 to the placement groove 14 through the circulation pipe 4 and keeps the oocyte preservation liquid in the placement groove 14 to flow, an oocyte preservation pipe 2 is arranged in the placement groove 14, permeation holes are further arranged on the surface of the oocyte preservation pipe 2, multiple groups of oocyte preservation pipes 2 are arranged, and one oocyte is arranged in a single oocyte preservation pipe 2;
as shown in fig. 3, a sealing ring is arranged between the lower box body 11 and the upper box body 12, the lower box body 11 and the upper box body 12 are fixedly connected through screws, the air suction holes 13 are communicated with the placing groove 14, a one-way valve 9 is arranged in a passage between the air suction holes 13 and the placing groove 14 and used for preventing air from entering the placing groove 14, the lower box body 11 is also provided with two accessory grooves 15, the two accessory grooves 15 are respectively arranged at two sides of the placing groove 14, the liquid storage box 5, the micro-flow pump 6 and the flow valve 7 are arranged in one of the accessory grooves 15, and the liquid storage box 5 is arranged in the other accessory groove 15;
preferably, the diameter of the surface penetration hole of the oocyte preservation tube 2 is less than 0.4 mm, and the oocyte preservation tube 2 is a circular tube in the embodiment;
the oocyte preservation tube 2 is connected to the box body 1 through a fixing ring 3, the fixing ring 3 is a mesh tube with the same outer diameter as the oocyte preservation tube 2, and the fixing ring 3 is inserted into the bottom of the placing groove 14;
the circulating pipe 4 is a capillary glass pipe, in the embodiment, the liquid storage box 5 is a glass test tube, the micro-flow pump 6 is connected with the liquid storage box 5 through the circulating pipe 4, and a plurality of groups of liquid outlets are arranged in the placing groove 14 of the circulating pipe 4, so that liquid in the placing groove 14 flows uniformly;
preferably, the flow pipe 4 is also provided with a flow valve 7 for controlling the flow rate of the oocyte preservation solution in the flow pipe 4, and preferably, the flow rate of the liquid in the flow pipe 4 is 0.2-0.5 mm/s;
the nutrient solution collecting tank 8 is a glass test tube, and a plurality of groups of liquid inlets are arranged in the placing groove 14 through the circulating pipe 4 between the nutrient solution collecting tank 8 and the placing groove 14.
Example 2
As shown in fig. 1 to 2, a normal-temperature oocyte preservation device includes a box body 1, the box body 1 is divided into a lower box body 11 and an upper box body 12, the lower box body 11 is hermetically connected with the upper box body 12, a placement groove 14 is arranged in the lower box body 11, the placement groove 14 is connected with a micro-flow pump 6 and a nutrient solution collecting tank 8 through a circulation pipe 4, the micro-flow pump 6 is communicated with a liquid storage box 5, oocyte preservation liquid is stored in the liquid storage box 5, the micro-flow pump 6 conveys the oocyte preservation liquid in the liquid storage box 5 to the placement groove 14 through the circulation pipe 4 and keeps the oocyte preservation liquid in the placement groove 14 to flow, an oocyte preservation pipe 2 is arranged in the placement groove 14, permeation holes are further arranged on the surface of the oocyte preservation pipe 2, multiple groups of oocyte preservation pipes 2 are arranged, and one oocyte is arranged in a single oocyte preservation pipe 2;
preferably, in this embodiment, a sealing plug is disposed at the mouth of the oocyte preservation tube 2, so as to prevent the change of the external environment from affecting the oocytes in the other oocyte preservation tubes 2 when one oocyte preservation tube 2 is taken;
other structures in this embodiment are the same as those in embodiment 1.
In summary, the working principle of the invention is as follows:
the preserving fluid in the standing groove 14 slowly flows for preserving fluid has new preserving fluid to replace throughout in the standing groove 14, thereby it is less to make the preserving fluid concentration change in the standing groove 14, thereby make the save in-process, the oocyte environmental change is less, and 2 surfaces of oocyte preservation tubes are equipped with the infiltration hole, make the oocyte preserving fluid composition of standing groove 14 supply to the oocyte preservation tube 2 all the time in, thereby reduce the environmental change in the oocyte preservation tube 2.
It should be particularly noted that, in this application, it is the innovation point of this application that the preservation environment of oocyte is maintained by reducing the concentration of oocyte preservation solution through slow flow and the mode that has new oocyte preservation solution to supply all the time, and it has effectively solved the problem that oocyte preservation device has the risk of damaging the oocyte among the prior art easily.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, are only for the convenience of description of the invention and to simplify the description, and do not indicate or imply that the referred device or element must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. The oocyte normal-temperature preservation device is characterized by comprising a box body (1), wherein the box body (1) is divided into a lower box body (11) and an upper box body (12), the lower box body (11) is hermetically connected with the upper box body (12), a placement groove (14) is arranged in the lower box body (11), the placement groove (14) is connected with a micro-flow pump (6) and a nutrient solution collecting tank (8) through a circulation pipe (4), the micro-flow pump (6) is communicated with a liquid storage box (5), oocyte preservation liquid is stored in the liquid storage box (5), the micro-flow pump (6) conveys the oocyte preservation liquid in the liquid storage box (5) to the placement groove (14) through the circulation pipe (4) and keeps the oocyte preservation liquid in the placement groove (14) to flow, an oocyte preservation pipe (2) is arranged in the placement groove (14), permeation holes are further formed in the surface of the oocyte preservation pipe (2), and a plurality of groups of oocyte preservation pipes (2) are arranged, and one oocyte is placed in the single oocyte preservation tube (2).
2. The oocyte cryopreservation device according to claim 1, wherein a sealing ring is arranged between the lower box body (11) and the upper box body (12), the air suction hole (13) is communicated with the placement groove (14), and a one-way valve (9) is arranged in a passage between the air suction hole (13) and the placement groove (14).
3. The oocyte cryopreservation apparatus according to claim 1, wherein the lower case (11) is further provided with two attachment grooves (15), the attachment grooves (15) are respectively provided on both sides of the placement groove (14), the fluid reservoir (5), the micro-flow pump (6) and the flow valve (7) are provided in one of the attachment grooves (15), and the fluid reservoir (5) is provided in the other attachment groove (15).
4. The oocyte cryopreservation device according to claim 1, wherein the diameter of the surface penetration hole of the oocyte preservation tube (2) is less than 0.4 mm.
5. The oocyte cryopreservation device according to claim 1, wherein the flow tube (4) is provided with a plurality of groups of liquid outlets in the placement groove (14); a plurality of groups of liquid inlets are arranged in the placing groove (14) of the circulating pipe (4) between the nutrient solution collecting groove (8) and the placing groove (14).
6. The oocyte cryopreservation device according to claim 5, wherein the flow pipe (4) is further provided with a flow valve (7), and the flow rate of the liquid in the flow pipe (4) is 0.2-0.5 mm/s.
7. The oocyte cryopreservation apparatus according to any one of claims 1 to 6, wherein the oocyte preservation tube (2) is connected to the box body (1) through a fixing ring (3), the fixing ring (3) is a mesh tube having the same outer diameter as the oocyte preservation tube (2), and the fixing ring (3) is inserted into the bottom of the placement groove (14).
8. The oocyte cryopreservation device according to claim 7, wherein the mouth of the oocyte preservation tube (2) is provided with a sealing plug.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911155882.8A CN110754465A (en) | 2019-11-22 | 2019-11-22 | Oocyte normal atmospheric temperature save set |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911155882.8A CN110754465A (en) | 2019-11-22 | 2019-11-22 | Oocyte normal atmospheric temperature save set |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110754465A true CN110754465A (en) | 2020-02-07 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911155882.8A Withdrawn CN110754465A (en) | 2019-11-22 | 2019-11-22 | Oocyte normal atmospheric temperature save set |
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| Country | Link |
|---|---|
| CN (1) | CN110754465A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113180035A (en) * | 2021-04-27 | 2021-07-30 | 沭阳县人民医院 | Can draw save conveyer of oocyte fast |
-
2019
- 2019-11-22 CN CN201911155882.8A patent/CN110754465A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113180035A (en) * | 2021-04-27 | 2021-07-30 | 沭阳县人民医院 | Can draw save conveyer of oocyte fast |
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Application publication date: 20200207 |
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| WW01 | Invention patent application withdrawn after publication |