CN216219784U - Embryonic stem cell cryopreservation device for reproductive medicine research - Google Patents
Embryonic stem cell cryopreservation device for reproductive medicine research Download PDFInfo
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- CN216219784U CN216219784U CN202122916481.9U CN202122916481U CN216219784U CN 216219784 U CN216219784 U CN 216219784U CN 202122916481 U CN202122916481 U CN 202122916481U CN 216219784 U CN216219784 U CN 216219784U
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Abstract
The utility model discloses an embryonic stem cell cryopreservation device for reproductive medicine research, which belongs to the technical field of medical instruments and comprises a cryopreservation box body, wherein the front surface of the cryopreservation box body is provided with a placing groove, an embryonic stem cell placing vessel is placed in the placing groove, the outer side of the cryopreservation box body is provided with a controller, the rear surface of the cryopreservation box body is fixedly connected with a liquid nitrogen storage and conveying box, the liquid nitrogen storage and conveying box is provided with a nitrogen adding assembly, and the top end in the placing groove is provided with a liquid nitrogen concentration detector; through setting up the nitrogen subassembly, can freeze short-term storage when the device uses, can provide the liquid nitrogen again and carry out long-term storage, and through setting up stable assembly, can avoid placing embryonic stem cell's embryonic stem cell and place the ware and take place to rock when adding nitrogen, solved current device storage mode when using single and waste time and energy problem.
Description
Technical Field
The utility model relates to the technical field of medical instruments, in particular to an embryonic stem cell cryopreservation device for reproductive medicine research.
Background
Embryonic stem cells are isolated from early embryos or primitive gonads, have the characteristics of unlimited proliferation, self-renewal and multidirectional differentiation in vitro culture, can be induced to differentiate into almost all cell types of the body in both in vitro and in vivo environments, are remarkably different from ordinary cells, have specific growth characteristics and specific marks, such as very high alkaline phosphatase activity and surface antigens specific to embryo stages, and also have marks of high molecular weight glycoproteins TRA1-60 and TRA-1-81, and can be used for identifying the embryonic stem cells. When a differentiation inhibitor such as leukemia inhibitory factor is added into an in vitro culture system or the embryonic stem cells are cultured on a mouse embryonic fibroblast feeder layer, the embryonic stem cells can be clonally propagated, the karyotype is kept normal and stable for a long time, and the characteristic of no differentiation is not influenced by freezing and thawing.
When the embryonic stem cells are preserved, the embryonic stem cells are usually stored in a refrigerator at minus eighty degrees first, and then stored for a long time in a preservation box with liquid nitrogen, but the existing cryopreservation device in the market at present often has various different problems when being used.
For example, current cryopreservation device can only freeze the transient storage to embryonic stem cell, if need carry out the long-term storage, avoids the conversion storage device, wastes time and energy, great reduction the practicality of device.
SUMMERY OF THE UTILITY MODEL
In view of the defects of the prior art, the utility model aims to provide an embryonic stem cell cryopreservation device for reproductive medicine research, which needs to solve the problems that the device has the following advantages by improvement: the freezing storage and the liquid nitrogen storage are integrated, so that the practicability of the device is greatly improved.
In order to achieve the purpose, the utility model provides the following technical scheme:
the utility model provides an embryonic stem cell cryopreservation device of reproductive medicine research usefulness, includes cryopreservation case box, the standing groove has been seted up to the front surface of cryopreservation case box, the embryo stem cell is placed to the inside of standing groove and is placed the ware, the outside of cryopreservation case box is provided with the controller, the back fixed surface of cryopreservation case box is connected with liquid nitrogen storage delivery box, be provided with on the liquid nitrogen storage delivery box with the nitrogen component, the inside top of standing groove is provided with liquid nitrogen concentration detector, the inside of cryopreservation case box is provided with inhales the nitrogen component.
Through adopting above-mentioned technical scheme, through setting up with the nitrogen subassembly, can add nitrogen to the standing groove is inside when inside liquid nitrogen concentration of standing groove is not enough, inhale the nitrogen subassembly through the setting, can absorb the inside liquid nitrogen of standing groove to avoid when taking out embryonic stem cell, the inside liquid nitrogen of standing groove leaks and causes the problem of resource loss.
Further, the nitrogen adding assembly comprises a first connecting pipe, one end of the first connecting pipe is fixedly connected with the liquid nitrogen storage and conveying box, the other end of the first connecting pipe is fixedly connected with a cavity plate liquid nitrogen flow distribution plate, a plurality of second connecting pipes are fixedly connected to one side of the cavity plate liquid nitrogen flow distribution plate, a plurality of first electromagnetic valves are arranged on the outer sides of the second connecting pipes, one ends of the second connecting pipes are respectively penetrated in the plurality of placing grooves, and limiting assemblies are arranged on the top ends of the placing grooves.
Through adopting above-mentioned technical scheme, add the nitrogen subassembly through the setting, can add nitrogen to the standing groove is inside when the inside liquid nitrogen concentration of standing groove is not enough.
Further, inhale nitrogen subassembly includes that liquid nitrogen absorbs the bin, the bin is absorbed to liquid nitrogen set up in the inside of cryopreservation case box, one side fixedly connected with third connecting pipe of bin is absorbed to liquid nitrogen, the one end fixedly connected with air pump of third connecting pipe, be provided with the fourth connecting pipe on the air pump, the one end of fourth connecting pipe run through in the inside of standing groove.
Through adopting above-mentioned technical scheme, inhale the nitrogen subassembly through the setting, can absorb the inside liquid nitrogen of standing groove to avoid when taking out embryonic stem cell, the inside liquid nitrogen of standing groove leaks and causes the problem of resource loss.
Further, a second electromagnetic valve is arranged on the outer side of the fourth connecting pipe, and the second electromagnetic valve is used for controlling the fourth connecting pipe.
Through adopting above-mentioned technical scheme, through setting up the second solenoid valve, can control the opening and the closed condition of fourth connecting pipe.
Further, the back surface of the box body of the cryopreservation box is fixedly connected with a supporting plate, and the supporting plate is attached to the lower surface of the liquid nitrogen storage and conveying box.
Through adopting above-mentioned technical scheme, through setting up the backup pad, can support the second solenoid valve.
Further, the limiting assembly comprises a cavity rod, the cavity rod is fixedly connected to the top end of the inner portion of the placing groove, a T-shaped rod is movably sleeved on the lower surface of the cavity rod, one end of the T-shaped rod is fixedly connected with a supporting block, a pull rod is fixedly connected to the outer side of one end, far away from the supporting block, of the T-shaped rod, one end of the pull rod penetrates through the outer side of the cavity rod, and a sliding groove matched with the pull rod is formed in the outer side of the cavity rod.
Through adopting above-mentioned technical scheme, through setting up spacing subassembly, can avoid when adding nitrogen embryonic stem cell and place the ware and take place to rock, great increase the stability after embryonic stem cell is placed.
In conclusion, the utility model has the following beneficial effects:
1. through setting up the nitrogen subassembly, can be when the device uses, can freeze short-term storage, can provide the liquid nitrogen again and carry out long-term storage, great increase the practicality of device, and through setting up the stabilizing assembly, can avoid placing the embryonic stem cell of embryonic stem cell and place the ware and take place to rock when adding nitrogen, great increase the stability of device, solved current device storage mode when using single and the problem that wastes time and energy.
2. Through setting up a plurality of standing grooves, and all set up on every standing groove and add the nitrogen subassembly and inhale the nitrogen subassembly, can cut apart the management of placing embryonic stem cell, and can be when taking out single embryonic stem cell, through inhaling the nitrogen subassembly, absorb the inside liquid nitrogen of standing groove, when embryonic stem cell places, the inside to the standing groove is leading-in again with the absorbed liquid nitrogen, so that can make the embryonic stem cell of placing in the embryonic stem cell placing dish inside preserve inside suitable liquid nitrogen concentration, thereby avoid it to take place rotten, can make the inside liquid nitrogen concentration of standing groove reach the concentration that can long-term storage embryonic stem cell, great increase the practicality of device, and avoided the loss of cost.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without inventive exercise.
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic cross-sectional view of the present invention;
FIG. 3 is a rear view of the present invention;
FIG. 4 is a schematic diagram of the structure at A in FIG. 2 according to the present invention;
fig. 5 is a schematic side view of a cavity bar according to the present invention.
In the figure:
1. a freezing preservation box body; 2. a placement groove; 3. placing the embryonic stem cells in a dish; 4. a controller; 5. a liquid nitrogen storage and conveying box; 6. a first connecting pipe; 7. a cavity plate liquid nitrogen flow distribution plate; 8. A second connecting pipe; 9. a first solenoid valve; 10. a liquid nitrogen concentration detector; 11. a liquid nitrogen absorption storage tank; 12. a third connecting pipe; 13. an air pump; 14. a fourth connecting pipe; 15. a second solenoid valve; 16. a support plate; 17. a cavity rod; 18. a T-shaped rod; 19. a resisting block; 20. a pull rod; 21. a sliding groove.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example (b):
the present invention is described in further detail below with reference to figures 1-5.
Referring to fig. 1-5, the present invention provides a technical solution: an embryonic stem cell cryopreservation device for reproductive medicine research comprises a cryopreservation box body 1, wherein a placing groove 2 is formed in the front surface of the cryopreservation box body 1, an embryonic stem cell placing vessel 3 is placed in the placing groove 2, a controller 4 is arranged on the outer side of the cryopreservation box body 1, a liquid nitrogen storage and conveying box 5 is fixedly connected to the rear surface of the cryopreservation box body 1, a nitrogen adding assembly is arranged on the liquid nitrogen storage and conveying box 5, and a liquid nitrogen concentration detector 10 is arranged at the top end of the interior of the placing groove 2; the nitrogen adding assembly comprises a first connecting pipe 6, one end of the first connecting pipe 6 is fixedly connected with the liquid nitrogen storage and conveying box 5, the other end of the first connecting pipe 6 is fixedly connected with a cavity plate liquid nitrogen flow distribution plate 7, one side of the cavity plate liquid nitrogen flow distribution plate 7 is fixedly connected with a plurality of second connecting pipes 8, the outer sides of the plurality of second connecting pipes 8 are respectively provided with a first electromagnetic valve 9, one ends of the plurality of second connecting pipes 8 respectively penetrate through the inside of the plurality of placing grooves 2, and the top end of the inside of the placing grooves 2 is provided with a limiting assembly; the back surface of the freezing storage box body 1 is fixedly connected with a supporting plate 16, and the supporting plate 16 is attached to the lower surface of the liquid nitrogen storage and conveying box 5; spacing subassembly includes cavity pole 17, and cavity pole 17 fixed connection has cup jointed T shape pole 18 on the inside top of standing groove 2, the one end fixedly connected with of T shape pole 18 supports piece 19, and T shape pole 18 is kept away from the one end outside fixedly connected with pull rod 20 that supports piece 19, and the one end of pull rod 20 runs through in the outside of cavity pole 17, and the sliding tray 21 of looks adaptation is seted up for the position of pull rod 20 in the cavity pole 17 outside.
In this embodiment, by providing the liquid nitrogen concentration detector 10, the model of the liquid nitrogen concentration detector 10 is ZBK-1000, the liquid nitrogen concentration inside the placing groove 2 can be detected, when the liquid nitrogen concentration is insufficient, by opening the corresponding first electromagnetic valve 9, the liquid nitrogen stored inside the liquid nitrogen flow distribution plate 7 of the cavity plate can be introduced into the placing groove 2 through the second connecting pipe 8 for adding nitrogen, so that the embryonic stem cells placed inside the embryonic stem cell placing dish 3 can be stored inside the appropriate liquid nitrogen concentration, thereby preventing the deterioration of the embryonic stem cells, greatly increasing the practicability of the device, before the embryonic stem cell placing dish 3 is placed inside the placing groove 2, by pulling the pull rod 20 upwards, the embryonic stem cells can slide inside the sliding groove 21, so that the support block 19 is located at the top end of the embryonic stem cell placing dish 3, when the embryonic stem cell placing dish 3 is placed inside the placing groove 2, the pull rod 20 is loosened, the resisting block 19 moves downwards under the action of gravity until contacting with the outer side of the embryonic stem cell placing dish 3, so that the problem that the embryonic stem cell placing dish 3 shakes is avoided when nitrogen is added, and the stability of the embryonic stem cell placing inside the embryonic stem cell placing dish 3 is greatly improved.
As shown in fig. 2, a nitrogen absorption assembly is arranged inside the freezing preservation box body 1; the nitrogen absorption assembly comprises a liquid nitrogen absorption storage box 11, the liquid nitrogen absorption storage box 11 is arranged inside the freezing storage box body 1, one side of the liquid nitrogen absorption storage box 11 is fixedly connected with a third connecting pipe 12, one end of the third connecting pipe 12 is fixedly connected with an air pump 13, the type of the air pump 13 is RB, a fourth connecting pipe 14 is arranged on the air pump 13, and one end of the fourth connecting pipe 14 penetrates through the inside of the placing groove 2; a second solenoid valve 15 is disposed at an outer side of the fourth connection pipe 14, and the second solenoid valve 15 is disposed to control the fourth connection pipe 14.
In this embodiment, when taking the container 3 for placing the embryonic stem cells, first, the second electromagnetic valve 15 and the air pump 13 are controlled by the controller 4, so that the second electromagnetic valve 15 is in an open state, the liquid nitrogen in the container 2 can be pumped into the liquid nitrogen absorption storage box 11 through the fourth connecting pipe 14 and the third connecting pipe 12 by the air pump 13 for storage, when the embryonic stem cells are placed into the container 3 for placing the embryonic stem cells again, and the container 2 needs to be placed for storage again, by opening the second electromagnetic valve 15, under the action of the air pump 13, the liquid nitrogen stored in the container 11 can be introduced into the container 2 for storage of the embryonic stem cells again, thereby providing external conditions for storage of the embryonic stem cells again.
The working principle is as follows: by arranging the plurality of placing grooves 2 and arranging the nitrogen adding component and the nitrogen absorbing component on each placing groove 2, the problem that the preservation time of the embryonic stem cells is influenced due to the fact that liquid nitrogen in the placing grooves 2 in the existing device leaks out when the embryonic stem cells are taken out can be avoided;
when the embryonic stem cell placing vessel 3 for placing the embryonic stem cells is taken, firstly, the second electromagnetic valve 15 and the air pump 13 are controlled by the controller 4, so that the second electromagnetic valve 15 is in an open state, liquid nitrogen in the placing groove 2 can be pumped into the liquid nitrogen absorbing and storing box 11 through the fourth connecting pipe 14 and the third connecting pipe 12 by the air pump 13 for storage, when the embryonic stem cells are placed into the embryonic stem cell placing vessel 3 again and need to be placed into the placing groove 2 for storage again, the liquid nitrogen stored in the liquid nitrogen absorbing and storing box 11 can be introduced into the placing groove 2 by opening the second electromagnetic valve 15 under the action of the air pump 13, and the liquid nitrogen concentration in the placing groove 2 can be detected by arranging the liquid nitrogen concentration detector 10;
when the concentration of the liquid nitrogen is insufficient, the liquid nitrogen stored in the liquid nitrogen flow distribution plate 7 of the cavity plate can be introduced into the placing groove 2 through the second connecting pipe 8 to be added with the nitrogen by opening the corresponding first electromagnetic valve 9, so that the embryonic stem cells placed in the embryonic stem cell placing vessel 3 can be stored in the proper liquid nitrogen concentration, the deterioration of the embryonic stem cells is avoided, and the practicability of the device is greatly improved;
in addition, before placing the embryonic stem cell placing dish 3 into placing groove 2 inside, through upwards pulling pull rod 20, make its inside slip at sliding tray 21, thereby make to support piece 19 to be in the top that the embryo stem cell placed dish 3, place the dish 3 and place at placing groove 2 inside back when the embryonic stem cell, loosen pull rod 20, under the effect of gravity, support piece 19 downstream, contact until placing the outside of dish 3 with the embryonic stem cell, thereby can be when adding nitrogen, avoid the embryonic stem cell to place the problem emergence that dish 3 took place to rock, great increase the embryonic stem cell and placed and place the inside stability of dish 3 at the embryonic stem cell.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the utility model. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The foregoing shows and describes the general principles, essential features, and advantages of the utility model. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the utility model as claimed.
Claims (6)
1. The utility model provides an embryonic stem cell cryopreservation device of reproductive medicine research usefulness, includes cryopreservation case box (1), standing groove (2) have been seted up to the front surface of cryopreservation case box (1), embryonic stem cell placing vessel (3) have been placed to the inside of standing groove (2), the outside of cryopreservation case box (1) is provided with controller (4), its characterized in that: the rear surface of the cryopreservation box body (1) is fixedly connected with a liquid nitrogen storage conveying box (5), a nitrogen adding assembly is arranged on the liquid nitrogen storage conveying box (5), a liquid nitrogen concentration detector (10) is arranged at the top end of the inside of the placing groove (2), and a nitrogen absorbing assembly is arranged inside the cryopreservation box body (1).
2. The cryopreservation device for embryonic stem cells for reproductive medicine research according to claim 1, wherein: the nitrogen adding assembly comprises a first connecting pipe (6), one end of the first connecting pipe (6) is fixedly connected with the liquid nitrogen storage and conveying box (5), the other end of the first connecting pipe (6) is fixedly connected with a cavity plate liquid nitrogen flow distribution plate (7), one side of the cavity plate liquid nitrogen flow distribution plate (7) is fixedly connected with a plurality of second connecting pipes (8) in a plurality, the outer sides of the second connecting pipes (8) are provided with first electromagnetic valves (9) in a plurality, one ends of the second connecting pipes (8) are respectively penetrated through the plurality of second connecting pipes in the placing groove (2), and the top end of the placing groove (2) is provided with a limiting assembly.
3. The cryopreservation device for embryonic stem cells for reproductive medicine research according to claim 1, wherein: inhale nitrogen subassembly and include that liquid nitrogen absorbs bin (11), liquid nitrogen absorb bin (11) set up in the inside of cryopreservation case box (1), one side fixedly connected with third connecting pipe (12) of bin (11) is absorbed to liquid nitrogen, the one end fixedly connected with air pump (13) of third connecting pipe (12), be provided with fourth connecting pipe (14) on air pump (13), the one end of fourth connecting pipe (14) run through in the inside of standing groove (2).
4. The cryopreservation device for embryonic stem cells for reproductive medicine research according to claim 3, wherein: and a second electromagnetic valve (15) is arranged on the outer side of the fourth connecting pipe (14), and the second electromagnetic valve (15) is used for controlling the fourth connecting pipe (14).
5. The cryopreservation device for embryonic stem cells for reproductive medicine research according to claim 1, wherein: the rear surface of the cryopreservation box body (1) is fixedly connected with a supporting plate (16), and the supporting plate (16) is attached to the lower surface of the liquid nitrogen storage and conveying box (5).
6. The cryopreservation device for embryonic stem cells for reproductive medicine research according to claim 2, wherein: spacing subassembly includes cavity pole (17), cavity pole (17) fixed connection in the inside top of standing groove (2), T shape pole (18) have been cup jointed in the lower surface activity of cavity pole (17), the one end fixedly connected with of T shape pole (18) supports piece (19), T shape pole (18) are kept away from support the one end outside fixedly connected with pull rod (20) of piece (19), the one end of pull rod (20) run through in the outside of cavity pole (17), the sliding tray (21) of looks adaptation is seted up for the position of pull rod (20) in the cavity pole (17) outside.
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| CN202122916481.9U CN216219784U (en) | 2021-11-25 | 2021-11-25 | Embryonic stem cell cryopreservation device for reproductive medicine research |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115669716A (en) * | 2022-11-08 | 2023-02-03 | 浙江海洋大学 | Quick-freezing device for fish balls and operation method thereof |
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2021
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115669716A (en) * | 2022-11-08 | 2023-02-03 | 浙江海洋大学 | Quick-freezing device for fish balls and operation method thereof |
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