CN202269321U - Low-temperature storage carrier for cell - Google Patents
Low-temperature storage carrier for cell Download PDFInfo
- Publication number
- CN202269321U CN202269321U CN2011203434502U CN201120343450U CN202269321U CN 202269321 U CN202269321 U CN 202269321U CN 2011203434502 U CN2011203434502 U CN 2011203434502U CN 201120343450 U CN201120343450 U CN 201120343450U CN 202269321 U CN202269321 U CN 202269321U
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- cell
- carrier
- groove
- low
- temperature storage
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Abstract
The utility model discloses a low-temperature storage carrier for a cell, which comprises a cell support plate, wherein the tip end of the cell support plate is dug with a groove, and the bottom of the groove is thinner than the surrounding slide glass. The carrier further comprises an extension rod, a connecting rod and a handle, wherein the cell support plat, the extension rod, the connecting rod and the handle are successively connected. The low-temperature storage carrier for a cell, which is provided by the utility model, is characterized in that the strength of the low-temperature storage carrier is big enough to support the self structure of the carrier because the support plates around the rectangular groove are thicker and have proper width, and the low-temperature storage carrier does not deform when loading the egg cells. Because the thickness of the film of the rectangular groove is lowered to be below 10mu m from the traditional 100 mu m, the thermal resistance of the film is lowered to 10% of the original thermal resistance to greatly improve the cooling rate and perform the active action on the vitrification storage of the egg mother cell loaded on the film. According to the measurement, the cooling rate of the improved carrier is improved by 8% than the cooling rate of the carrier which is not improved.
Description
Technical field
The utility model belongs to technical field of medical instruments, relates to a kind of low temperature and preserves carrier, relates in particular to a kind of carrier that egg mother cell vitrifying low temperature is preserved that is used for.
Background technology
With the reproductive cell long preservation of animal and human's class, be human common ideal always, scientist just attempts preserving sperm with straw as far back as the sixties in last century, and has obtained good effect, and sets up human and animal's sperm bank, is used for the long preservation of sperm.But egg cell is because own vol is bigger, and specific surface area is little, and cooling rate is slower in process of cryopreservation; Form ice crystal easily, cause ice crystal puncture organelle and cell membrane thereof, damaged the structure of cell; Cause cell death, the preservation effect of egg cell is not very desirable all the time.
Modern freezing theory is thought; Can let egg cell lower temperature rapidly through adding cryoprotector with the raising rate of temperature fall, form the unordered solid of vitreousness, this solid does not contain ice crystal; Can cell membrane and organelle damage, can realize the long preservation of egg cell.In order to improve cooling rate, domestic and international research personnel have invented freezing ring (Cryoloop), freezing lid (cryotop) and freezing leaf (cryoleaf) as carrier, realize that the vitrifying of egg cell is preserved.Wherein freezing lid ratio juris is exactly that the egg mother cell that contains frozen solution is added drop-wise to the slide glass surface that is called freezing lid; Utilize capillary to draw unnecessary frozen solution then; Make cell volume minimum; Then freezing lid is together inserted in the liquid nitrogen together with egg mother cell, make the cell realization vitrifying of lowering the temperature rapidly.Find that through Theoretical Calculation in refrigerating process, the thermal resistance of egg cell mainly concentrates on the slide glass of freezing lid, reduce the thickness of slide glass, can effectively improve cooling rate, more be of value to the egg mother cell vitrifying.But because the restriction of material self intensity will become very soft if the slide glass thickness of freezing lid is reduced to below the 100 μ m, be not enough to support himself structure, so freezing lid slide glass commonly used all more than 100 μ m, can not continue to reduce thickness.Limited the rate of temperature fall of egg cell; In order to guarantee the vitrifying effect; Must improve the concentration of frozen solution, because the toxicity of frozen solution self, the frozen solution of higher concentration has great destruction to egg cell; Therefore, existing freezing lid slide glass is not fine to the preservation effect of egg mother cell.
The utility model content
The utility model technical problem to be solved is: provide a kind of cell low temperature to preserve carrier, can improve the preservation effect of cell.
For solving the problems of the technologies described above, the utility model adopts following technical scheme:
A kind of cell low temperature is preserved carrier, and said carrier comprises the cell support plate, takes a groove at cell support plate tip, and the bottom thickness of groove is than slide glass is thin on every side.
As a kind of preferred version of the utility model, said carrier further comprises extension rod, connecting rod, handle, and said cell support plate, extension rod, connecting rod, handle connect successively.
As a kind of preferred version of the utility model, the cross section of said groove is rectangle.
As a kind of preferred version of the utility model, the thickness of said bottom portion of groove is smaller or equal to 10 μ m.
The beneficial effect of the utility model is: the low temperature that the utility model proposes is preserved carrier and on original freezing lid carrier basis, is improved; Take about wide 150 μ m at original polyethylene material cell support plate tip, the rectangle like groove of long 1cm, this depth of groove reaches 90 μ m; The polythene material of its bottom is 10 μ m even thinner; Because the support plate around the rectangular recess is thicker, width is (thickness＞100 μ m, width＞120 μ m) suitably; Its intensity is enough to support the slide glass self structure, and indeformable when loading egg cell.Because the thickness of rectangular recess part film is reduced to below the 10 μ m from 100 traditional μ m, it is original 10% that its thermal resistance also is reduced to, and improved rate of temperature fall greatly, preserves positive role for the vitrifying that loads egg mother cell on it.According to measuring, the slide glass rate of temperature fall after the improvement has not improved 8% than improved.
Description of drawings
Fig. 1 preserves the structural representation of carrier for the utility model low temperature.
Fig. 2 is the enlarged drawing of groove part.
Fig. 3 is that B-B to groove part is to cutaway view.
Fig. 4 is the structural representation of the protective sleeve of carrier.
Embodiment
Specify the preferred embodiment of the utility model below in conjunction with accompanying drawing.
Embodiment one
See also Fig. 1, Fig. 2, Fig. 3, the utility model has disclosed a kind of cell low temperature and has preserved carrier, and said carrier comprises cell support plate 1, takes a groove at cell support plate 1 tip, and the bottom thickness of groove (C place) is than slide glass (E place) is thin on every side.In the present embodiment, the cross section of said groove is rectangle, and the thickness of said bottom portion of groove is smaller or equal to 10 μ m (thickness of conventional grooves bottom is generally about 100 μ m).
Said carrier further comprises extension rod 2, connecting rod 3, handle 4, and said cell support plate 1, extension rod 2, connecting rod 3, handle 4 connect successively.
The utility model in use; The egg mother cell that needs freezing processing or other cell are carried out pre-treatment, and concrete processing method is omitted, and uses the suction pipe of suitable thickness to be loaded into the groove part of freezing lid support plate at microscopically in the cell of handling; Use capillary to draw unnecessary frozen solution; The freezing lid carrier of carrier is dropped in the liquid nitrogen together with cell, with tweezers gripping protective sleeve (Fig. 4 is the structural representation of the protective sleeve of carrier in the present embodiment), for freezing lid has overlapped cover; With global transfer to bigger liquid nitrogen container, immerse the liquid nitrogen long preservation.Rewarming is opened protective sleeve with tweezers in liquid nitrogen if desired, and the slide glass part of freezing lid is inserted rapidly in the rewarming liquid, returns to room temperature through subsequent treatment, carries out other associative operation and cultivation.Use this support plate to carry out cell freezing, its survival rate improves more than 10% than contrast.
In sum, the low temperature that the utility model proposes is preserved carrier and on original freezing lid carrier basis, is improved, and takes about wide 150 μ m at original polyethylene material cell support plate tip; The rectangle like groove of long 1cm; This depth of groove reaches 90 μ m, and the polythene material of its bottom is 10 μ m even thinner, because the support plate around the rectangular recess is thicker; Width is (thickness＞100 μ m suitably; Width＞120 μ m), its intensity is enough to support the slide glass self structure, and indeformable when loading egg cell.Because the thickness of rectangular recess part film is reduced to below the 10 μ m from 100 traditional μ m, it is original 10% that its thermal resistance also is reduced to, and improved rate of temperature fall greatly, preserves positive role for the vitrifying that loads egg mother cell on it.According to measuring, the slide glass rate of temperature fall after the improvement has not improved 8% than improved.
Here description of the utility model and application is illustrative, is not to want the scope of the utility model is limited in the above-described embodiments.Here the distortion of the embodiment that is disclosed and change are possible, and the replacement of embodiment is known with the various parts of equivalence for those those of ordinary skill in the art.Those skilled in the art are noted that under the situation of spirit that does not break away from the utility model or substantive characteristics, and the utility model can be with other form, structure, layout, ratio, and realize with other assembly, material and parts.Under the situation that does not break away from the utility model scope and spirit, can carry out other distortion and change here to the embodiment that is disclosed.
Claims (4)
1. a cell low temperature is preserved carrier, it is characterized in that said carrier comprises the cell support plate, takes a groove at cell support plate tip, and the bottom thickness of groove is than slide glass is thin on every side.
2. cell low temperature according to claim 1 is preserved carrier, it is characterized in that:
Said carrier further comprises extension rod, connecting rod, handle, and said cell support plate, extension rod, connecting rod, handle connect successively.
3. cell low temperature according to claim 1 is preserved carrier, it is characterized in that:
The cross section of said groove is rectangle.
4. cell low temperature according to claim 1 is preserved carrier, it is characterized in that:
The thickness of said bottom portion of groove is smaller or equal to 10 μ m.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011203434502U CN202269321U (en) | 2011-09-14 | 2011-09-14 | Low-temperature storage carrier for cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011203434502U CN202269321U (en) | 2011-09-14 | 2011-09-14 | Low-temperature storage carrier for cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202269321U true CN202269321U (en) | 2012-06-13 |
Family
ID=46189718
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011203434502U Expired - Fee Related CN202269321U (en) | 2011-09-14 | 2011-09-14 | Low-temperature storage carrier for cell |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202269321U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103444699A (en) * | 2013-08-01 | 2013-12-18 | 吕祁峰 | Miniature carrier system for cryopreservation of biological materials, bucket and cryopreservation method |
| CN106489915A (en) * | 2016-12-28 | 2017-03-15 | 中信湘雅生殖与遗传专科医院有限公司 | Freezing carrier mechanism and the freezing carrier unit including the freezing carrier mechanism |
-
2011
- 2011-09-14 CN CN2011203434502U patent/CN202269321U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103444699A (en) * | 2013-08-01 | 2013-12-18 | 吕祁峰 | Miniature carrier system for cryopreservation of biological materials, bucket and cryopreservation method |
| CN103444699B (en) * | 2013-08-01 | 2015-07-29 | 吕祁峰 | A kind of miniature base system, well-bucket and the cryopreservation methods frozen for biomaterial |
| CN106489915A (en) * | 2016-12-28 | 2017-03-15 | 中信湘雅生殖与遗传专科医院有限公司 | Freezing carrier mechanism and the freezing carrier unit including the freezing carrier mechanism |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120613 Termination date: 20120914 |