CN103444699B - A kind of miniature base system, well-bucket and the cryopreservation methods frozen for biomaterial - Google Patents
A kind of miniature base system, well-bucket and the cryopreservation methods frozen for biomaterial Download PDFInfo
- Publication number
- CN103444699B CN103444699B CN201310333111.XA CN201310333111A CN103444699B CN 103444699 B CN103444699 B CN 103444699B CN 201310333111 A CN201310333111 A CN 201310333111A CN 103444699 B CN103444699 B CN 103444699B
- Authority
- CN
- China
- Prior art keywords
- bar
- frozen
- bar cylinder
- year
- well
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a kind of miniature base system frozen for biomaterial and application thereof, form primarily of frozen unit, described frozen unit comprises a year bar, carries bar cover and carry bar cylinder; Carry in bar front end insertion year bar cover and form smooth elongated load units; be loaded into together again and carry in bar cylinder; described year bar cylinder is the blank pipe of at least bottom end closure, mean outside diameter < 8.5mm, and the top or the sidewall that carry bar cylinder are provided with the dress bar mouth and loading protection thereof and taking-up technology that load units can be put into and taken out from year bar cylinder.Miniature base system provided by the invention makes, not affecting the space basis even more increasing ease of use decreased exponentially shared by micro-Frozen Biological, significantly to have saved the floor space needed for stored frozen.Be particularly useful for frozen as embryo of micro-biomaterial.
Description
Technical field
The present invention relates to a kind of miniature base system frozen for biomaterial, particularly relate to a kind of biological to carry out with medical domain micro-biological material vitrified frozen or other freezing time a set of sample loading attachment of using and application thereof.
Background technology
At present, known freezing carrier system has many kinds, can be divided into frozen tubular type and frozen rod-type two class by main load units.The former is that its one end is cecum usually in cylinder shape or partial cylinder type tubular container, and the other end is closed with screw thread or click on fashion by block.This container is usually comparatively large, and volume generally more than 1 milliliter, and is stored on the layer-stepping carrier of liquid nitrogen container by grid mostly for box-packed year.This form of the frozen employing of current most cells; Latter is rod-like device, comprise: the straw shape vessel form that (1) is elongated, can the biological material specimens of frozen 1-5 hectolambda, (2) pipe end is by the elongated form of further drawing-down, utilize elongated portion to carry out frozen, can be used for the frozen of several microliters of sample, (3) also have elongated rod-shaped non-vessel form, there is frozen carrier sheet its front end, this sheet can adhere to the biomaterial within loading number microlitre.It is frozen that latter two form all belongs to micro-example, applies more in the ovum cultivated in vitro or the glass frozen preservation of embryo.For the glass freezing of ovum or embryo, also have the trace of frozen loop type frozen, namely with the liquid containing ovum that microfilament ring adheres to or embryo, then be positioned in tubular container and protect and frozen.
But the frozen device of current above-mentioned various trace (number microlitre within) sample does not take relatively less memory space than constant (hundreds of microlitre causes several milliliters), causes storage area application efficiency low.This reason is wherein: the loading of micro-example identifies simple and operate the needs such as facility with taking also must meet, therefore except sample carrier loading station itself, also must there be other dependency structure and device, helping the loading of sample, close, mark, that classification stores and accesses is convenient etc., the coupling of these structures and device at present defines larger space.
To carry out freezing to the embryo of culture in vitro in Issues of Human Assisted Reproductive Technologies, the glass freezing of its embryo usually adopts frozen rod type (the following special embryo cryopreservation that claims carries bar or is called for short a year bar), the non-vessel form of such as elongated rod-shaped, be the most save space of current embryo cryopreservation and operation also form easily, comprise and carry a bar (one end has frozen), carry bar cover, carry bar cylinder, carry bar cylinder cap and carry bar tub system.Fig. 1 is conventional carriers system schematic, forms by carrying bar 1 ', carrying bar cover 2 ', carry bar cylinder 3 ', carry bar cylinder cap 4 ' and carrying bar tub 5 '.As shown in Figure 1, carry bar 1 ' front end to insert and carry in bar cover 2 ', then be loaded into together and carry in bar cylinder 3 ', and carry bar cylinder 3 ' and carried in bar tub 5 ' by clamping, its top has the bar cylinder cap 4 ' that carries by carrying bar tub 5 ' clamping to cover, and prevents from carrying dropping out of bar 1 '.Wherein carry on bar frozen for placing embryo, bar cover uploaded by its one end quilt cover placing embryo, do not damage to protect this section by external force; Carry bar cylinder then to carry bar for loading and carry bar cover; An aluminium alloy frame is also had namely to carry bar tub, for clamping year bar cylinder and year bar cylinder cap (carrying bar cylinder cap not drop out outside cylinder for the protection of article in cylinder).Carry on bar and can post biomaterial information labels.Carry bar tub upper end and can stick the information labels such as frozen position, form a frozen unit.Generally the multiple samples deriving from same patient being carried bar is loaded in same or multiple frozen unit, and the embryo in different patient source can not load in mixture in same frozen unit, is beneficial to identity marks and resolution.
As above carry bar tub and clamping carry bar cylinder and carry bar cylinder cap not only to have an appointment the thickness of 1-1.5 cm diameter, also have about 29 centimetre lengths altogether.In this covering device, what generation maximum space took has two aspects: (1) carries a bar tub, because it must than carry bar cylinder greatly just can hold clamping year bar cylinder and with carry bar cylinder etc. large carry bar cylinder cap, and this clamping necessity is that will fix a year bar cylinder cap makes it to be in a year bar cylinder openend, do not drop out to protect the bar that carries carried in bar cylinder; (2) being carry bar cover, because it must the one end of bar being placed embryo is large just can put this end than carrying, thus usually creating than carrying the thicker space hold of bar.This makes to carry bar cylinder must have comparatively thick (about 1 centimetre) internal diameter for meeting usual useful load (6-8 embryo needs 3-4 root to carry bar).
In general, the embryo of a patient at least needs to carry bar tub with one, need according to transplanting, the frozen amount that every root carries bar is 1-3 piece of embryo, and a quantity of carrying bar wound packages year bar is between 1 to 4, namely when client need frozen about 8 and following embryo time, it need take a support bar tub, then needs to take 2 or more carry bar tub more than 8.Loading when this carrier system can facilitate freezing, finds rapidly when also conveniently thawing and carries bridge and carry bar from the extraction bar cylinder of carrying of carrying bridge clamping from liquid nitrogen container.But, although this system uses conveniently upper, occupy many spaces.In the liquid nitrogen container of 47 liters of 10 well-buckets, each well-bucket only about can put into 26-28 support bar tub, at least needs a support bar tub to calculate, then the embryo of frozen at most about 28 patients of each well-bucket with each patient.Then the liquid nitrogen container of 47 liters of 10 well-buckets can deposit at most the embryo of about 280 patients.Concerning a relatively large supplementary reproduction center, if on average monthly get the ovum cycle more than 500, even if consider to count the space that embryo of thawing discharges in, its liquid nitrogen container needed for newly-increased frozen embryo may need increase by average every month, within several years, gets off and will cause taking of extra space.This causes restriction to the development of a supplementary reproduction mechanism.Therefore, current micro-Frozen Biological device and the space efficiency utilization of corresponding loading technology relatively low, also cannot meet the frozen and development of the sample of large-scale.
On the other hand, freezing or thaw operation time, the bar tub aluminum alloy material that carries be in liquid nitrogen volatilization steam fog freezes compared with steam in the easier condensation air of other plastic material, and this ice that is condensed after usually causing label segment to expose liquid nitrogen certain hour hides, and causes the inconvenience of operation.
In prior art, the thickest structure of frozen unit is for carrying bar tub, i.e. an aluminium alloy frame, its specification and shape are same, even so that one carries bar and also needs to carry with 4 that bar is the same takies a frozen unit, 5 carried bar and then need with 2 frozen unit, than 4 years many one times of bars.These load all Existential Space wastes.In the present embodiment, carry bar cylinder and be made of plastics, do not need unified specification as aluminium alloy frame, and different size and shape can be made according to actual weight of load, rationally to take up room.3-4 root is needed to carry bar because great majority load 6-8 embryo, can design with 3 that to carry bar useful load be minimum gauge carries bar cylinder, what also increase design 4,5 useful loads carries bar cylinder, and design different year bar cylinder cross-sectional area and shape according to useful load difference, except traditional circle, also comprise ellipse, fan-shaped, square etc.Such 1-3 root can be contained in 3 specification cylinders, and 4-5 root is contained in 4,5 specification cylinders respectively, 6 and above then can with larger design, or adopt the bar cylinder combination of carrying of many above-mentioned specifications to load, as 6 pieces points of 23 dresses, 7 pieces points 13 and 14 dresses, make full use of space; Also can adopt single dress or 2 dress schemes, although take may be slightly some more for its freezing time space, the space can accelerated when thawing is vacateed.Such as single dress scheme, that namely designs minimum only accommodation load units (carry bar and carry bar cover) carries bar cylinder.This all can empty each freezing unit taken out at every turn after making to thaw, and also just will not put back to liquid nitrogen container again by freezing unit, can simplify thaws flow process and accelerate space vacates.
Figure 11 is year bar cylinder shape of cross section designed component schematic diagram of carrier system of the present invention.Carry bar cylinder 4 and can hold multiple load units, can be made of plastics, preferably clear or translucent plastic are made, and different size can be formulated according to actual weight of load: as shown in A in Figure 11, what design was maximum loading specification with 3 load units carries bar cylinder 4,3 load units in it can be in the minimum occupancy state of triangle position automatically, therefore adopt circular cross section design; As shown in B in Figure 11, design year bar cylinder 3 that 4 load units are maximum loading specification, 4 load units in it can be in the minimum occupancy state of diamond shaped positions automatically, therefore oval cross section can be adopted to design; As shown in C in Figure 11, design year bar cylinder 3 that 5 load units are maximum loading specification, 5 load units in it can be in the minimum occupancy state of pentagon position automatically, therefore circular cross section can be adopted to design; Also can design different other year bar cylinder shape of cross section, also comprise fan-shaped, square etc.Such 1-3 root can be contained in carrying in bar cylinder 3 of 3 gauge lattice, and what 4-5 root was contained in 4,5 gauge lattice respectively carries in bar cylinder 3,6 and then can with larger design, or adopt the bar cylinder combination of carrying of many above-mentioned specifications to load above, makes full use of space.Meanwhile, the well-bucket space of a former unit can be separated into multiple (as 3-5) space, to reduce frozen unit number in each space, thus improves the speed finding specific markers.
Summary of the invention
In order to overcome existing micro-Frozen Biological device and the corresponding deficiency of loading technology in storage area utilization ratio thereof, the invention provides a kind of miniature base system frozen for biomaterial and Cryopreservation Technology thereof, with guarantee freezing loading, mark, storage, resolution and taking-up and to thaw etc. each simple and convenient while, the space shared by carrier arrangement can also be reduced more significantly, make each liquid nitrogen container amount of storage (embryo vitrifying freeze for supplementary reproduction then refers to the case load of storing samples) increase about 3-4 doubly.
First aspect of the present invention is to provide a kind of miniature base system frozen for biomaterial, comprises frozen unit, and described frozen unit comprises a year bar, carries bar cover and carry bar cylinder; Wherein,
Within described year, bar cover is hollow structure, and at least one end is provided with the opening be communicated with described hollow structure;
The frozen portion that described year bar comprises the body of rod and be connected with body of rod bottom, carry be provided with socket part between club shaft and frozen portion, the outer surface that club shaft place is being carried in socket part has certain distance with a year club shaft outer surface, this distance equals the wall thickness carrying bar cover opening part, described frozen portion is inserted into the hollow structure interior formation load units carrying bar cover, and described year bar cover is fixed by socket on the socket part of carrying bar;
Described year bar cylinder is that at least bottom end closure, mean outside diameter < 8.5mm(mean outside diameter refer to that maximum outside diameter adds the mean value of minimum outer diameter, be preferably 4-8.5mm, be more preferably 5-7mm) blank pipe, carrying that the top of bar cylinder or sidewall be provided with can by load units from carrying the dress bar mouth put into and take out bar cylinder.
Wherein, between described year bar cylinder inwall and load units, gap can be there is, or a year bar cylinder inwall contacts with load units, there is not gap therebetween, but load units can slide in year bar cylinder.
Wherein, described socket part can be a part of carrying club shaft or frozen.
Described socket part is preferably continues progressively to reduce to frozen direction, more preferably slightly progressively reduces to frozen direction.
In one preferred embodiment, described dress bar mouth is for being arranged at least one notch load units can put into and taken out from year bar cylinder on the sidewall of year bar cylinder, the length of described load units is greater than notch bottom to the length of carrying bottom bar cylinder, but is less than notch centre position to the length of carrying bottom bar cylinder.
Wherein, described notch is preferably strip.
Wherein, the length of described notch can be the length being more than or equal to described load units; Even also can be less than the length of load units, and the load units carried in bar cylinder can be made to be taken out from carrying in bar cylinder by described notch.
Wherein, the length of described load units is preferably less than and is more preferably 1/4 from the 1/3(of distance notch bottom, is more preferably 1/5, is more preferably 1/6, be more preferably 1/7, be more preferably 1/8) length bottom notch position place to year bar cylinder.
Preferably, described notch hypomere is the jaws part that A/F reduces, and the length of described load units is greater than jaws part bottom to the length of carrying bottom bar cylinder, but is less than jaws part top to the length of carrying bottom bar cylinder.
Preferably, described frozen unit also comprises lever, and lever slides carrying the position of carrying more than bar cylinder notch bottom in bar cylinder, thus changes and block length, to open or close dress bar mouth to notch.Lever can all or only be in and carries in bar cylinder by its hypomere.
Further preferably, the top end opening of described year bar cylinder, lever hypomere inserts carry in bar cylinder from carrying bar cylinder top end opening, and pin epimere increases thick, pin epimere external diameter is exceeded carry bar cylinder top end opening internal diameter with fixing pin and locate pin to block position to the maximum of notch.
Preferably, described pin is hard straight blank pipe, and when being all in year bar cylinder for lever, its bottom is blind end, and when for lever, only its hypomere is in year bar cylinder, its two ends are blind end; Breeder tube is provided with at the end face of blind end or peripheral wall surfaces.
Preferably, the bottom of described year bar cylinder is equipped with metal and increases the weight of body.
Another preferred embodiment in, described year bar wound packages bar mouth for be arranged on carry bar cylinder top carry bar cylinder nozzle; Described frozen unit also comprises sleeve, sleeve is the long tube of upper end closed lower ending opening, described top, sleeve upper end and/or perisporium are provided with ventilation hole, described sleeve hypomere is provided with and can inserts from carrying bar cylinder nozzle the linkage section carried in bar cylinder, described linkage section from bottom to top external diameter increases gradually, and the maximum outside diameter of linkage section is more than or equal to the internal diameter of (preferably equaling) year bar cylinder nozzle thus makes sleeve can be inserted into a year bar cylinder nozzle internal fixtion, to close dress bar mouth and to extend frozen unit; Insert the sleeve lower end after fixing is greater than load units length to the distance of carrying bottom bar cylinder; Within described year, be equipped with the iron block that can slide in year bar cylinder bottom bar cylinder.
Wherein, between the lower end of the linkage section of described year bar cylinder inwall and sleeve, there is gap, and described gap is not enough to insert load units.
Gap can be there is between described year bar cylinder inwall and iron block, and described gap is not enough to insert load units; Or carry bar cylinder inwall to contact with iron block, there is not gap therebetween, but iron block can slide in year bar cylinder.
When to above-mentioned year, bar wound packages bar mouth thawed for being arranged on the frozen unit carrying bar cylinder top, described miniature base system also comprises magnetic location dimple, described magnetic location dimple is provided with the groove that can hold and carry bar cylinder, on the sidewall of described groove, the position of distance bottom portion of groove at least more than 3mm (be preferably at least more than 6mm, be more preferably at least more than 9mm) is mounted with magnet piece.
Another preferred embodiment in, the frozen portion of described year bar is fine hollow tubular structure or strip constructions.
Wherein, the external diameter of described fine hollow tubular structure is 0.3-3mm, is more preferably 0.8-2.5mm, is more preferably 1.2-2.2mm.
Wherein, the internal diameter of described fine blank pipe is 0.1-2.8mm, is more preferably 0.5-2.2mm, is more preferably 0.8-1.8mm.
Described strip feature cross-section is preferably spill.
Wherein, described list structure is groove type or arc-like sheet, and forms end at end.Described end can be wedge angle or fillet.
In any one carrier system that the present invention is above-mentioned, the cross section of described year bar cylinder can be comprise circular various shape, as circle, ellipse, semicircle, half elliptic, rectangular circle (rectangular broadside is arc-shaped edges), polygon, not even regular shape, described polygon is as triangle, quadrangle, pentagon, hexagon etc.; But be preferably circular oval or rectangular circle, most preferably be circle.
In any one carrier system that the present invention is above-mentioned, the part of described year bar except frozen can be entity or the hollow shape of cylinder, partial cylinder, prism, cylindroid etc., and preferably cylindrical.
In any one carrier system that the present invention is above-mentioned, described year bar cover can be the shapes such as cylinder, prism, cylindroid, and preferably cylindrical.
Preferably, the body of rod of described year bar is tubulose.
Another preferred embodiment in, the top of the body of rod of described year bar is provided with label paste section, and described label paste section is the thin bar thinner than the body of rod or the flat-shaped body narrower than the body of rod.
The duct such as ventilation hole, breeder tube, liquid-through hole of the upper top of all above-mentioned tube chambers all has the effect that the through-flow and liquid nitrogen vapor of liquid nitrogen is discharged; can enter liquid nitrogen and form cryoprotection when freezing, or when thawing, discharged liquid nitrogen vapor pressure prevents blast.
Another preferred embodiment in, load one or more load units in described year bar cylinder.The bar cylinder that carries that can design different inner diameters and shape as required loads with the load units of applicable different radical or combination loading.
Another preferred embodiment in, described carrier system also comprise carry bar cover a stacking help slot; The stacking of described year bar cover helps slot at least to comprise three walls.
Preferably, described year bar is stacked and is helped slot to comprise two double walls and a diapire, and three walls connect between two, the pyrometric cone groove of a composition opening.
Further, preferably, the dihedral angle formed between two double walls can be wedge angle or fillet, and the arc radius of described fillet is less than the arc radius of frozen groove.
Wherein, the stacking of described year bar cover helps slot to be preferably polygonal taper, as pyrometric cone, quadrangular pyramid etc., and most preferably is triangular pyramidal.
Another preferred embodiment in, described carrier system also comprises well-bucket, for placing frozen unit.
A second aspect of the present invention is to provide a kind of well-bucket being applicable to the side leakage type storing miniature frozen unit, the bottom of described well-bucket is closed, the sidewall of described well-bucket at least preferably exceeds bottom 5mm higher than bottom 3mm(, more preferably exceed bottom 7mm, more preferably exceed bottom 10mm) more than part be provided with liquid-through hole.
Preferably, well-bucket inside is provided with multiple perpendicular to the shim bottom well-bucket, well-bucket interior separation is become multiple space (as 3-5), to reduce frozen unit number in each space, thus improves the speed finding specific markers.
Preferably, be provided with Upright post base in described well-bucket, described Upright post base comprises the base be arranged on bottom well-bucket and the column be vertically set on base.
Preferably, described Upright post base is provided with outside column a circle smooth sagging sagging end, at the bottom of peripheral outer ring of raising for plane, the described sagging end, radiate centered by column at the bottom of outer ring and be provided with multiple base draw-in groove that shim base snaps in of allowing linearly, the bottom of draw-in groove is equal with the sagging end, with the level difference more than at least 1 millimeter at the sagging end at the bottom of the draw-in groove degree of depth and outer ring.This design can make the base draw-in groove in well-bucket a lot.When the freezing unit amount that causes former interval of constantly thawing reduces more, shim can be withdrawn from draw-in groove and can move to again new draw-in groove insert in well-bucket.This when not affecting freezing unit separation in former interval, can realize interval compression, reserving new space for new separation.
Preferably, described column also can be provided with multiple side draw-in groove that a certain side of shim snaps in of allowing perpendicular to base.
Preferably, described base (at the bottom of plane) be provided with multiple be connected with side draw-in groove one_to_one corresponding allow the base draw-in groove that shim base snaps in.
Wherein, preferably, described column is central type column, and the top margin length of described shim equals the distance of bottom land to well-bucket inwall of side draw-in groove, thus produces fixed effect better further.
Preferably, the side of the lower width of described shim never checkpost, inserting side groove reduces (side keeping checkpost, shim inserting side groove is neat limit, perpendicular to base), and the width reduced at least is greater than the degree of depth of side draw-in groove.This make the shim that is plugged upwards withdrawn from expose well-bucket limit to the side reduced time, to direction outside side, shim can be taken out from side draw-in groove, and mobile shim is to another draw-in groove within well-bucket, then side direction snaps in side draw-in groove, is then inserted into base draw-in groove downwards.When the freezing unit amount that causes former interval of constantly thawing reduces more, in this well-bucket, the operation of mobile shim when not affecting that in former interval, freezing unit is separated, can realize interval compression, reserving new space for new separation.
Wherein, preferably, described column also can be inclined central type column, and the width of each shim described is that never homonymy checkpost trench bottom extends to the length of well-bucket inwall along base draw-in groove straight line, therefore width disunity.
Described shim can be different colours.
3rd aspect of the present invention is to provide a kind of cryopreservation methods of biomaterial, adopts above-mentioned miniature base system and above-mentioned well-bucket, comprises the following steps:
Step 1, provides above-mentioned miniature base system, is placed in frozen portion by biomaterial frozen in liquid nitrogen, and bar cover carried by cover, composition load units, then load units is put into a year bar cylinder from the dress bar mouth carrying bar cylinder, closes dress bar mouth, forms frozen unit;
Step 2, provides the well-bucket described in above-mentioned any one, and well-bucket inner space is separated into multiple interval, is placed in by well-bucket in liquid nitrogen container;
Step 3, frozen in the well-bucket that what frozen unit step 1 obtained loaded that step 2 provides be in liquid nitrogen container.In one preferred embodiment, described in described step 1, cover is carried bar cover and is comprised:
Step 1.1, provides a year bar cover stacking to help slot; Described year bar cover is stacked the walls helping slot at least to comprise three direct contacts and is surrounded two double walls and a diapire, intersects triconic angle, the end; Triconic angle, the end is placed on position, the most end, makes that the fillet between two double walls is oblique to be raised; To carry bar cover be placed in single or multiplely carry bar cover a stacking help slot, make carry bar cover a bottom be resisted against on diapire; Carrying bar is enclosed within Action of Gravity Field and two double walls stop, one of its foot carries the center position that bar cover nature is in two double wall fillets;
Step 1.2, is close to and carries bar cover by carrying the front end of bar and stack and help on two double wall walls of slot two double wall fillet, frozen portion then can auto-alignment be in two double wall fillet center positions carry bar cover entrance, will carrying bar mobile to carrying bar cover, being inserted in year bar overlaps.
The present invention has given up year bar tub taken up space most in conventional carriers system, and can do less by carrying a bar cylinder diameter because decreasing year bar overlapping the thickness after uploading bar cover, within this year simultaneously, bar cylinder is designed to multiple size and shape, to adapt to multiple frozen needs under minimum occupy-place, these make the overall storage capacity of liquid nitrogen container expand about original 3-4 to doubly.Meanwhile, usually have the set of condensation ice to hide the inconvenience on label because this frozen unit solves conventional art without the need to aluminium alloy carries bar tub, also stack owing to carrying bar cover the design ap-plication helping slot, the upper cover carrying bar when making frozen operates more simple and fast.
The invention has the beneficial effects as follows, making, not affecting the space basis even more increasing ease of use decreased exponentially shared by micro-Frozen Biological, significantly to have saved the floor space needed for stored frozen.Be particularly useful for frozen as glass freezing embryo etc. of micro-biomaterial.
Accompanying drawing explanation
Fig. 1 is conventional carriers system and loads schematic flow sheet;
Fig. 2 is miniature base system in an embodiment of the present invention and loads schematic flow sheet;
Fig. 3 is miniature base system in an embodiment of the present invention and loading and dismounting schematic flow sheet;
Fig. 4 be existing well-bucket with well-bucket provided by the invention compare schematic diagram, A is existing well-bucket, and bottom surface is provided with liquid-through hole, and B is well-bucket provided by the invention, and sidewall is provided with liquid-through hole;
Fig. 5 is well-bucket provided by the invention and assembling flow path schematic diagram;
Fig. 6 carries the stacking of bar cover to help slot and using method schematic diagram in an embodiment of the present invention;
Fig. 7 carries bar frozen portion structural representation in an embodiment of the present invention;
Fig. 8 carries the structural representation in bar connecting portion and frozen portion insertion year bar cover in an embodiment of the present invention
Fig. 9 is frozen portion groove structure schematic diagram in an embodiment of the present invention;
Figure 10 carries bar inserting stacking groove for the frozen portion of groove type described in Fig. 9 uses described in Fig. 6 and enters to the schematic diagram in year bar cover;
Figure 11 is in multiple load units situation, carries bar cylinder difformity cross sectional representation.
Embodiment
Below in conjunction with accompanying drawing, by specific embodiment, the detailed description of shape is entered to the present invention.
Embodiment 1
The present embodiment by former one end open type carry that bar cylinder 3 ' attenuates instead and open from the side strip window and the notch 31(that lengthen fill bar mouth) and at least bottom end closure form carry bar cylinder 3; Containing picking and placeing of bar to carrying bar cylinder, is by carrying out in tweezers from the side notch 31; That gives up external force clamping type carries bar cylinder cap 4 ', make hypomere into and insert the pin 4 carrying in bar cylinder and be subject to friction fixing from the top end opening carrying bar cylinder, the open length of notch 31 is controlled in the occupy-place length at notch 31 place with pin, its open longer time can carry out carrying a bar 1(load units) loading or unloading operation, then can live carry bar 1 carrying the slip in bar cylinder 3 by shelves time open shorter.So just carry bar cylinder cap without the need to external, thus also given up with regard to corresponding year bar tub for clamping year bar cylinder cap taken up space most.Simultaneously, carry bar cylinder itself also serve bar tub of publishing originally play a part as the frozen unit of independence having different biological sample origin marking, namely mark in a year bar cylinder upper end, still can be convenient to identify and pick and place as bar tub top mark of publishing originally, form independently frozen unit.Such one multiplex, when not affecting original ease-to-operate, eliminate the large quantity space needed for a peripheral matched device.And, plastic material because new frozen unit carries bar cylinder, low heat conductivity, can not produce because label segment exposes liquid nitrogen certain hour the condensation of water in air vapour and freeze, solving current aluminium alloy and carry bar tub usually because condensation ice hides the inconvenience of label.
With reference to Fig. 2, the carrier system that the present embodiment provides forms primarily of a year bar 1, year bar cover 2, year bar cylinder 3, lever 4, the bottom (frozen portion 12) of carrying bar is inserted in year bar cover 2, then is loaded in year bar cylinder 3 together, and lever 4 hypomere inserts in year bar cylinder 3 from carrying bar cylinder 3 top end opening.
Carry the blank pipe that bar cover 2 is upper end open, lower end closed.
Carry the frozen portion 12 that bar 1 comprises the body of rod 11 and is connected with the body of rod 11 bottom, frozen portion 12 is inserted into carry in bar cover 2 and forms load units.
Carry that bar cylinder 3 be bottom end closure, the external diameter of upper end open is that 7mm(can be designed to other external diameter sizes according to actual needs) blank pipe, the sidewall carrying bar cylinder 3 is provided with the notch 31 of strip load units can put into and taken out from year bar cylinder 3.
The hypomere of notch 31 is the jaws part 311 that opening reduces, and the length of described load units is greater than jaws part 311 bottom to the length of carrying bottom bar cylinder 3, but is less than jaws part 311 top to the length of carrying bottom bar cylinder 3.
Carry the top end opening of bar cylinder 3, lever 4 hypomere inserts in year bar cylinder 3 from carrying bar cylinder 3 top end opening, slide carrying the position of carrying more than bar cylinder notch 31 bottom in bar cylinder 3, thus change length is blocked, to open or close dress bar mouth (i.e. notch 31) to notch 31; Pin 4 epimere increases thick, pin 4 epimere external diameter is exceeded carry bar cylinder 3 top end opening internal diameter with fixing pin 4 and locate pin 4 pairs of notches 31 block position.
Pin 4 is hard straight blank pipe, and its two ends are blind end, is provided with breeder tube at the end face of blind end or peripheral wall surfaces.In use, fill liquid nitrogen in blank pipe, to remove the buoyancy that air causes, be beneficial to sinking, simultaneously more filling liquid nitrogen more help freezing unit can postpone the liquid nitrogen volatilization dry time, maintenance cryogenic freezing state being taken out when liquid nitrogen is transported.
The top of carrying the body of rod 11 of bar is provided with label paste section 13, and label paste section 13 is the thin bar thinner than the body of rod or the flat-shaped body narrower than the body of rod.
With reference to Fig. 7 and 8, carry be provided with socket part 14 between club shaft 11 and frozen portion 12, the outer surface that club shaft 11 place is being carried in socket part 14 has certain distance with year club shaft 11 outer surface, this distance equals to carry the wall thickness that bar overlaps 2 opening parts, make to carry bar cover 2 after bar 1 uploaded by cover, form the straight rod-type without evagination with a year bar 1, save the space of usually carrying bar cover and taking because year bar periphery will be held.
But like this, year frozen end of bar sample must have been caused to reduce and carried a bar cover opening and will reduce, this can cause the difficulty year frozen end top of bar sample being visually observed when year bar cover opening inserts location.For this reason, further design locates insertion apparatus automatically with non-naked eyes and method overcomes:
One end, the i.e. frozen portion 12 of carrying bar frozen and stored biological sample will keep certain diameter, if frozen portion is frozen of strip constructions, then frozen is made into groove type, and as shown in Figure 7, and top 15 is pointed.Simultaneously carrier system shown in the present embodiment comprises one and carries the stacking of bar cover and help slot, and Fig. 6 carries the stacking of bar cover to help slot dependency structure, component and method of operating schematic diagram thereof in a kind of preferred embodiment of the present invention.As shown in Figure 7,8, the one end of carrying the frozen sample of bar 1 comprises socket part 14, frozen portion 12 and end, frozen portion 15.The direction in the frozen portion of outer radial, socket part 14 phases down, and is convenient to like this and carries a bar and overlap 2 and be locked gradually, and carries bar 1 after being locked and overlap 2 with a year bar and form straight docking because external diameter is identical, without evagination; Carry the groove type that the frozen portion 12 of bar 1 is strip, and its top is groove is sheared the pointed end 15 of formation, micro-biological sample is such as in the groove at namely 1-3 embryo be placed on this rear portion, end 15, frozen portion close.This groove structure can block frozen embryo, from operation by the danger of grazing.Meanwhile, arrange a triangular pyramidal as shown in Figure 6 and carry the stacking of bar cover and help slot 6, comprise two double walls 61 and bottom surface 62, the grooved of the one side open surrounded, the splicing of two walls 61 surrounds dihedral angle.When carry a bar cover stacking help slot 6 as shown in Figure 6 oblique placement time, the multiple years bar covers 2 wherein stacked can be made automatically neatly to be positioned corner, bottom by Action of Gravity Field, wherein one of bottommost carries bar cover 2 always straight two double walls 61 being close to dihedral angle, and when needing an insertion year bar to overlap 2 after carrying bar 1 and installing biological sample, then as shown in figs. 6 and 10, the two side walls 61 that the stacking of bar cover helps the dihedral angle of slot 6 can be carried by carrying club shaft 11 outer wall concora crush, maybe by the recess carrying the frozen portion 12 of bar front end sheet strip in the face of and push down and carry bar cover and stack and help the two side walls 61 of the dihedral angle of slot 6, to carry a bar overlap 2 directions insert go, at this moment because frozen portion 12 spill makes the tip, frozen portion 15 on its top unsettled by automatically raising, can not contact and carry the both sides double wall 61 that the stacking of bar cover helps slot 6, and the shape of its design and yardstick make this frozen portion most advanced and sophisticated 15 to carry within the opening of bar cover 2 by auto-alignment, which ensure that the frozen portion 12 after running business into particular one still can and more convenient without the need to insert with examining carry bar cover 2.
Embodiment 2
The present embodiment by former one end open type carry that bar cylinder 3 ' attenuates instead and lengthen from top end opening namely carry bar cylinder nozzle and bottom end closure and be equipped with the form of movable iron block at cylinder inner bottom part carry bar cylinder 3; The taking-up of bar is contained then: up and raise and carry bar and expose top and carry that bar cylinder nozzle carries out by magnetic location dimple drive activity iron block to carrying bar cylinder; That gives up external force clamping type carries bar cylinder cap 4 ', and what have hypomere linkage section can insert from carrying bar cylinder nozzle the sleeve 5 carrying in bar cylinder and be subject to friction fixing, to close dress bar mouth and to extend frozen unit to exceeding the dark length of liquid nitrogen container well-bucket bucket instead.Thus, also just give up year bar tub for clamping year bar cylinder cap taken up space most., mark in sleeve upper end meanwhile, still can be convenient to identify and pick and place as bar tub top mark of publishing originally.Like this when not affecting original ease-to-operate, eliminate the large quantity space needed for a peripheral matched device.And, because sleeve 5 is plastic materials, low heat conductivity, when it is tiltedly placed in liquid nitrogen box, the barrel exposing liquid nitrogen part can not affect by its liquid nitrogen, the Conduction At Low Temperature of part is too large, namely can not as aluminium alloy carries bar tub low temperature, therefore can not produce because label segment exposes liquid nitrogen certain hour and solidify icing to the excessive condensation of water in air vapour.Which solve current aluminium alloy and carry bar tub usually because condensation ice hides the inconvenience of label.
With reference to Fig. 3, the carrier system that the present embodiment provides forms primarily of a year bar 1, year bar cover 2, year bar cylinder 3, sleeve 5, the bottom (frozen portion 12) of carrying bar 1 is inserted in year bar cover 2, be loaded into year bar cylinder 3 from the bar cylinder nozzle that carries carrying bar cylinder 3 top together again, sleeve 5 hypomere inserts from year bar cylinder nozzle carrying bar cylinder 3 top and carries in bar cylinder 3.
Carry bar cover 1 described in the present embodiment and carry bar 2 and carry a bar described in embodiment 1 and overlap 1 identical with year bar 2.
Year bar cylinder 3 of the present embodiment is bottom end closure, the external diameter of upper end open is the blank pipe of 6mm, and the upper end open of carrying bar cylinder forms year bar cylinder nozzle (immediate shipment bar mouth) load units can put into and taken out from year bar cylinder.
Sleeve 5 is the long tube of upper end closed lower ending opening, described sleeve 5 hypomere is provided with and inserts from carrying bar cylinder nozzle the linkage section 51 carried in bar cylinder, described linkage section 51 from bottom to top external diameter increases gradually, and the maximum outside diameter of linkage section 51 equals to carry the internal diameter of bar cylinder nozzle thus sleeve 5 can insert and carries a bar cylinder nozzle internal fixtion, to close dress bar mouth and to extend frozen unit; Insert sleeve 5 lower end after fixing is greater than load units length to the distance of carrying bottom bar cylinder 3.
Carry the iron block 32 being equipped with bottom bar cylinder 3 and can sliding in year bar cylinder 3.
Carry and can there is gap between bar cylinder inwall and iron block 32, and described gap is not enough to insert load units; Or carry bar cylinder inwall to contact with iron block 32, there is not gap therebetween, but iron block can slide in year bar cylinder.
When needing load units to take out in year bar cylinder, available attraction carries iron block 32 upward sliding in bar cylinder, drives load units to expose and carries a bar cylinder nozzle.
In one preferred embodiment, described miniature base system also comprises magnetic location dimple 7, described magnetic location dimple is provided with the groove that can hold and carry bar cylinder, and on the sidewall of described groove, the position of distance bottom portion of groove more than at least 3 millimeters is mounted with magnet piece.When needing load units to take out in year bar cylinder, year bar cylinder 3 is placed in the groove of magnetic location dimple, the magnet piece in recess sidewall can cause the iron block 32 carried in bar cylinder to be up to magnet positions, and band dynamic load bar exposes a year bar cylinder nozzle.
Year bar cylinder of the present embodiment also can hold multiple load units, can be made of plastics, and can formulate different size according to actual weight of load, as shown in figure 11.
In above-described embodiment 1 or 2, the frozen portion of carrying bar comprises frozen of strip constructions, or is the frozen microtubule of fine hollow tubular structure.When for fine blank pipe, its external diameter is 0.3-3mm, and preferred external diameter is 1mm, and internal diameter is 0.8mm, and during frozen use, micro-biological sample is placed in fine blank pipe as namely 1-3 embryo be inhaled into.
Embodiment 3
Miniature base system provided by the invention attenuates owing to carrying bar cylinder, and when frozen, the amount of liquid nitrogen of carrying in bar cylinder is few, and the liquid nitrogen when extracting well-bucket in well-bucket is very fast have been leaked from bottom mesh.This makes a small amount of liquid nitrogen carried in bar cylinder be in non-liquid nitrogen environment and easily volatilize completely, and at this moment the frozen position of biomaterial may just not be in the protection of liquid nitrogen, easily causes improper the thawing of biomaterial and makes frozen failure.
With reference to Figure 4 and 5, existing well-bucket 8 ' for sidewall close, bottom is provided with netted liquid-through hole 81 ', present embodiments provide a kind of well-bucket 8 being applicable to store the side leakage type of miniature freezing unit, the bottom of described well-bucket is closed, and the part at least higher than bottom more than 3mm on the sidewall of described well-bucket is provided with liquid-through hole 81.When well-bucket is enhanced the liquid nitrogen surface exceeding liquid nitrogen container, the time is done in the volatilization that bottom well-bucket, the non-liquid nitrogen leaking height can delay a small amount of liquid nitrogen carried in bar cylinder.
In one preferred embodiment, well-bucket inside is provided with multiple perpendicular to the shim 91 bottom well-bucket, well-bucket interior separation is become multiple (as 3-5) space, to reduce frozen unit number in each space, thus improves the speed finding specific markers.
In a further preferred embodiment, Upright post base 9 is provided with in described well-bucket, described Upright post base 9 comprises the base 92 be arranged on bottom well-bucket and the column 93 be vertically set on base 92, wherein, described column 93 is provided with multiple side draw-in groove 94 that a certain side of shim 91 snaps in of allowing perpendicular to base, base 92 be provided with multiple be connected with side draw-in groove 94 one_to_one corresponding allow the base draw-in groove 95 that shim 91 base snaps in.
The top margin length of shim 91 equals the distance of bottom land to well-bucket 8 inwall of side draw-in groove 94, thus produces fixed effect better further.
Another side of the lower width of shim 91 never checkpost, inserting side groove 94 reduces (keeping the side of shim inserting side mark draw-in groove perpendicular to base), and the width reduced at least is greater than the degree of depth of side draw-in groove 94.This make the shim 91 that is plugged upwards withdrawn from expose well-bucket limit to the side reduced time, to direction outside side, shim 91 can be taken out from side card 94 groove, and within well-bucket mobile shim to another draw-in groove unit (being made up of the side draw-in groove be communicated with and base draw-in groove), side direction snaps in side draw-in groove 94 again, is then inserted into base draw-in groove 95 downwards.When the freezing unit amount that causes former interval of constantly thawing reduces more, in this well-bucket, the operation of mobile shim when not affecting that in former interval, freezing unit is separated, can realize interval compression, reserving new space for new separation.
Carry out biomaterial frozen time, the miniature base system that embodiment 1 or embodiment 2 provide can be adopted, biomaterial is placed in frozen portion frozen in liquid nitrogen, bar cover carried by cover, composition load units, again load units is put into from the dress bar mouth carrying bar cylinder and carry bar cylinder, close dress bar mouth, form frozen unit; Well-bucket inner space is separated into multiple interval, well-bucket is placed in liquid nitrogen container; Frozen unit is loaded in the well-bucket be in liquid nitrogen container, frozen loading can be completed.
Above-described embodiment 1-3 can be used as the large quantity space of glass freezing saving that the human embryos vitrified frozen vector system reformed is human embryos (or ovum).
Embodiment 4
Some tissue as ovary tissue sheet, little cartilage sheet, hair root, tumour fritter or other particular organization's fritter in the processes such as scientific research, detection or other application, need single or micro-independence frozen time, with traditional normal capacity cryopreservation tube frozen usually cause thaw after be difficult to find and lose, cryopreservation resuscitation efficiency is low and take too much space when in enormous quantities independence is frozen.And adopt the above-mentioned human embryos vitrified frozen vector system reformed of the present invention, then can be described above, avoid the too much shortcoming that takes up room, and can also solve because the carrying scope in frozen portion is little thaw after be difficult to find and the problem of losing, also because the ramp rates of frozen portion freeze thawing is far away faster than the freeze thawing ramp rates of normal capacity cryopreservation tube, make frozen effective, recovery survival and Quality advance.Its technological system and operating process similar human embryos vitrified frozen vector system and technology, as described in front embodiment 1 and 2, change embryo's biological specimen into particular organization.
Embodiment 5
Some cell as the mankind's trace seminal fluid, testis wear sperm, Trace Blood cell or other specific few cells need in the processes such as scientific research, detection or other application trace independent frozen time, with traditional normal capacity cryopreservation tube frozen usually cause thaw after be difficult to find and lose, cryopreservation resuscitation efficiency is low and take too much space when independently frozen in enormous quantities.And adopt the above-mentioned human embryos vitrified frozen vector system reformed of the present invention, then can be described above, avoid the too much shortcoming that takes up room, and can also because of the carrying scope of frozen little and solve thaw after be difficult to find and the problem of losing, also because the ramp rates of frozen freeze thawing is far away faster than the freeze thawing ramp rates of normal capacity cryopreservation tube, make frozen effective, recovery survival and Quality advance.Its technological system and operating process similar human embryos vitrified frozen vector system and technology, as described in front embodiment 1 and 2, embryo is changed into specific cells drop, but recovery of thawing is by different.
Thawing of embodiment 1,2 and 4 is all insert the frozen portion 12 of carrying bar 1 in the thawing solution of pre-temperature, and wherein thawing solution keeps its temperature not affect by the too fast of room temperature often through more liquid measure.This is for this embodiment, then the few cells thawed can be made to be diffused in volume liquid and to be difficult to carry out specific cells searching, and such as testis is worn sperm and often only had several or dozens of, cannot regather in the liquid of about 1 milliliter.For this reason, for embodiment 5, its course of defrosting inserts the frozen portion 12 of carrying bar 1 in the culture dish mineral oil of pre-temperature, after the frozen drop in frozen portion melts, to be inserted under culture dish mineral oil in another medium drop again, make sample droplets and cell thereof in frozen portion 12 be dissolved into this new medium drop, then find at this new drop and to thaw under few cells carries out mirror further and the operation such as use.
Be described in detail specific embodiments of the invention above, but it is just as example, the present invention is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that the present invention is carried out and substituting also all among category of the present invention.Therefore, equalization conversion done without departing from the spirit and scope of the invention and amendment, all should contain within the scope of the invention.
Claims (18)
1. for the miniature base system that biomaterial is frozen, it is characterized in that, comprise frozen unit, described frozen unit comprises a year bar, carries bar cover and carry bar cylinder; Wherein,
Within described year, bar cover is hollow structure, and at least one end is provided with the opening be communicated with described hollow structure;
The frozen portion that described year bar comprises the body of rod and be connected with body of rod bottom, carry be provided with socket part between club shaft and frozen portion, the outer surface that club shaft place is being carried in socket part has certain distance with a year club shaft outer surface, this distance equals the wall thickness carrying bar cover opening part, described frozen portion is inserted into the hollow structure interior formation load units carrying bar cover, and described year bar cover is fixed by socket on the socket part of carrying bar;
Described year bar cylinder is the blank pipe of at least bottom end closure, mean outside diameter < 8.5mm, and the top or the sidewall that carry bar cylinder are provided with dress bar mouth load units can put into and taken out from year bar cylinder;
The dress bar mouth of described year bar cylinder is for being arranged on year bar cylinder nozzle carrying bar cylinder top; Described frozen unit also comprises sleeve, sleeve is the long tube of upper end closed lower ending opening, described top, sleeve upper end and/or perisporium are provided with ventilation hole, described sleeve hypomere is provided with and can inserts from carrying bar cylinder nozzle the linkage section carried in bar cylinder, described linkage section from bottom to top external diameter increases gradually, and the maximum outside diameter of linkage section is more than or equal to and carries the internal diameter of bar cylinder nozzle thus sleeve can insert and carries a bar cylinder nozzle internal fixtion, to close dress bar mouth and to extend frozen unit; Insert the sleeve lower end after fixing is greater than load units length to the distance of carrying bottom bar cylinder; Within described year, be equipped with the iron block that can slide in year bar cylinder bottom bar cylinder;
Described miniature base system also comprises magnetic location dimple, and described magnetic location dimple is provided with to hold and carries the groove of bar cylinder, and on the sidewall of described groove, the position of distance bottom portion of groove at least more than 3mm is mounted with magnet piece.
2. miniature base system according to claim 1, it is characterized in that, described dress bar mouth is for being arranged at least one notch load units can put into and taken out from year bar cylinder on the sidewall of year bar cylinder, the length of described load units is greater than notch bottom to the length of carrying bottom bar cylinder, but is less than notch centre position to the length of carrying bottom bar cylinder.
3. miniature base system according to claim 2, it is characterized in that, described notch hypomere is the jaws part that A/F reduces, and the length of described load units is greater than jaws part bottom to the length of carrying bottom bar cylinder, but is less than jaws part top to the length of carrying bottom bar cylinder.
4. miniature base system according to claim 2, is characterized in that, described frozen unit also comprises lever, and lever slides carrying the position of carrying more than bar cylinder notch bottom in bar cylinder, thus changes and block length, to open or close dress bar mouth to notch.
5. miniature base system according to claim 4, it is characterized in that, the top end opening of described year bar cylinder, lever hypomere inserts in year bar cylinder from carrying bar cylinder top end opening, pin epimere increases thick, pin epimere external diameter is exceeded carry bar cylinder top end opening internal diameter with fixing pin and locate pin to block position to notch.
6. the miniature base system according to claim 4 or 5, is characterized in that, described pin is hard straight blank pipe, its at least bottom be blind end, be provided with breeder tube at the end face of blind end or peripheral wall surfaces.
7. miniature base system according to claim 1, is characterized in that, the fine hollow tubular structure of to be strip constructions or external diameter be in described frozen portion 0.3-3mm.
8. miniature base system according to claim 7, is characterized in that, the cross section of described strip constructions is spill.
9. miniature base system according to claim 1, is characterized in that, described year club shaft is tubulose.
10. miniature base system according to claim 1, is characterized in that, the top of the body of rod of described year bar is provided with label paste section, and described label paste section is the thin bar thinner than the body of rod or the flat-shaped body narrower than the body of rod.
11. miniature base systems according to claim 10, is characterized in that, load one or more load units in described year bar cylinder.
12. miniature base systems according to claim 1, it is characterized in that, described miniature base system also comprises a year bar cover stacking and helps slot, and within described year, bar sleeve assists groove to comprise two double walls and a diapire, three walls connect between two, the pyrometric cone groove of a composition opening.
13. miniature base systems according to claim 1, is characterized in that, also comprise the well-bucket for placing frozen unit.
14. 1 kinds of well-buckets being applicable to the side leakage type of the miniature base system stored described in the arbitrary claim of claim 1-13, it is characterized in that, the bottom of described well-bucket is closed, and the sidewall of described well-bucket is at least provided with liquid-through hole higher than the part of bottom more than 3mm.
15. well-buckets according to claim 14, it is characterized in that, described well-bucket inside is provided with multiple perpendicular to the shim bottom well-bucket, and well-bucket interior separation is become multiple space, to reduce frozen unit number in each space, thus improve the speed of the frozen unit finding specific markers.
16. well-buckets according to claim 15, it is characterized in that, Upright post base is provided with in described well-bucket, described Upright post base comprises the base be arranged on bottom well-bucket and the column be vertically set on base, described Upright post base is provided with outside column a circle smooth sagging sagging end, at the bottom of peripheral outer ring of raising for plane, the described sagging end, radiate centered by column at the bottom of outer ring and be provided with multiple base draw-in groove that shim base snaps in of allowing linearly, the bottom of draw-in groove is equal with the sagging end.
17. well-buckets according to claim 15, it is characterized in that, Upright post base is provided with in described well-bucket, described Upright post base comprises the base be arranged on bottom well-bucket and the column be vertically set on base, and described column is provided with multiple side draw-in groove that a certain side of shim snaps in of allowing perpendicular to base.
The cryopreservation methods of 18. 1 kinds of biomaterials, is characterized in that, adopts the well-bucket described in any one in miniature base system according to claim 1 and claim 14-17, comprises the following steps:
Step 1, provides miniature base system according to claim 1, is placed in frozen portion by biomaterial frozen in liquid nitrogen, bar cover carried by cover, composition load units, then load units is put into a year bar cylinder from the dress bar mouth carrying bar cylinder, close dress bar mouth, form frozen unit;
Step 2, provides the well-bucket in claim 14-17 described in any one, and well-bucket inner space is separated into multiple interval, is placed in by well-bucket in liquid nitrogen container;
Step 3, frozen in the well-bucket that what frozen unit step 1 obtained loaded that step 2 provides be in liquid nitrogen container.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310333111.XA CN103444699B (en) | 2013-08-01 | 2013-08-01 | A kind of miniature base system, well-bucket and the cryopreservation methods frozen for biomaterial |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310333111.XA CN103444699B (en) | 2013-08-01 | 2013-08-01 | A kind of miniature base system, well-bucket and the cryopreservation methods frozen for biomaterial |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103444699A CN103444699A (en) | 2013-12-18 |
| CN103444699B true CN103444699B (en) | 2015-07-29 |
Family
ID=49727884
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310333111.XA Active CN103444699B (en) | 2013-08-01 | 2013-08-01 | A kind of miniature base system, well-bucket and the cryopreservation methods frozen for biomaterial |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103444699B (en) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103749436B (en) * | 2014-01-23 | 2016-04-13 | 江苏苏云医疗器材有限公司 | Closed embryo cryopreservation carries bar and preparation method thereof and using method |
| CN104719283A (en) * | 2015-03-24 | 2015-06-24 | 阳艳群 | Closed freezing carrier and use method thereof |
| CN104986447B (en) * | 2015-06-10 | 2017-08-11 | 陈子江 | Sample stored frozen pipe and sample stored frozen device |
| CN105191909B (en) * | 2015-10-22 | 2018-02-16 | 复旦大学附属肿瘤医院 | The cryopreservation methods and biological sample bank tissue freezing pipe of biological sample bank tissue |
| CN105918310A (en) * | 2016-06-27 | 2016-09-07 | 广西壮族自治区妇幼保健院 | Trace sperm freezing device with centrifugal function |
| CN106172372A (en) * | 2016-07-07 | 2016-12-07 | 中国科学院东北地理与农业生态研究所 | The making of a kind of sheep frozen spermatic fluid preserves integrated apparatus and application process thereof |
| CN105923530A (en) * | 2016-07-11 | 2016-09-07 | 阳艳群 | Novel multilayer liquid nitrogen tank short bucket |
| CN108496956B (en) * | 2018-03-30 | 2023-09-01 | 浙江大学 | Auxiliary safety docking device under liquid nitrogen of human embryo freezing carrier rod and application method of auxiliary safety docking device |
| CN108626630A (en) * | 2018-05-31 | 2018-10-09 | 浙江大学 | A kind of irradiation unit filling bar for observing embryo under liquid nitrogen |
| CN108801751B (en) * | 2018-06-08 | 2021-02-02 | 湖北省地质勘查装备中心 | Filling hopper for filling rod |
| CN108617641A (en) * | 2018-07-03 | 2018-10-09 | 浙江星博生物科技股份有限公司 | A kind of biological sample glass frozen cryopreservation device |
| CN110637809B (en) * | 2019-10-10 | 2024-02-23 | 广州辅康生物科技有限公司 | Support rod sleeve |
| CN113575572A (en) * | 2021-08-19 | 2021-11-02 | 上海理工大学 | Ultra-fast rewarming method for blood vessel vitrification preservation |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006013825A1 (en) * | 2004-08-06 | 2006-02-09 | Livestock Improvement Association Of Japan Inc | Sample container and crimping device for sample container |
| CN101272861A (en) * | 2005-09-28 | 2008-09-24 | Cryo生物系统股份有限公司 | Kit for packaging predetermined volume of substance to be preserved by cryogenic vitrification |
| CN202127748U (en) * | 2011-05-25 | 2012-02-01 | 中国水产科学研究院黄海水产研究所 | Simple auxiliary device for sperm cryopreservation by liquid nitrogen tank |
| CN202269321U (en) * | 2011-09-14 | 2012-06-13 | 上海理工大学 | Low-temperature storage carrier for cell |
| CN202514485U (en) * | 2011-12-29 | 2012-11-07 | 深圳市易瑞生物技术有限公司 | Carrying tool for vitrified cryopreservation of biological materials |
| CN202773904U (en) * | 2012-09-20 | 2013-03-13 | 石家庄市第四医院 | Biological material vitrification freezing carrier |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011140299A1 (en) * | 2010-05-06 | 2011-11-10 | Butler Brad K | Carrier comprising a seal for cyrogenic storage |
| CN203492657U (en) * | 2013-08-01 | 2014-03-26 | 吕祁峰 | Miniature carrier system for cryopreserving biological materials |
-
2013
- 2013-08-01 CN CN201310333111.XA patent/CN103444699B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006013825A1 (en) * | 2004-08-06 | 2006-02-09 | Livestock Improvement Association Of Japan Inc | Sample container and crimping device for sample container |
| CN101272861A (en) * | 2005-09-28 | 2008-09-24 | Cryo生物系统股份有限公司 | Kit for packaging predetermined volume of substance to be preserved by cryogenic vitrification |
| CN202127748U (en) * | 2011-05-25 | 2012-02-01 | 中国水产科学研究院黄海水产研究所 | Simple auxiliary device for sperm cryopreservation by liquid nitrogen tank |
| CN202269321U (en) * | 2011-09-14 | 2012-06-13 | 上海理工大学 | Low-temperature storage carrier for cell |
| CN202514485U (en) * | 2011-12-29 | 2012-11-07 | 深圳市易瑞生物技术有限公司 | Carrying tool for vitrified cryopreservation of biological materials |
| CN202773904U (en) * | 2012-09-20 | 2013-03-13 | 石家庄市第四医院 | Biological material vitrification freezing carrier |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103444699A (en) | 2013-12-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103444699B (en) | A kind of miniature base system, well-bucket and the cryopreservation methods frozen for biomaterial | |
| JP6114185B2 (en) | Improved micromanipulation and storage apparatus and method | |
| CN203913154U (en) | Carrying tool for use in vitrification cryo-preservation of biomaterial | |
| CN203255458U (en) | Glass slide storage box allowing slide to be looked up conveniently | |
| CN203492657U (en) | Miniature carrier system for cryopreserving biological materials | |
| CN203424216U (en) | Side-leakage type bucket for storing minitype freezing unit | |
| CN203618631U (en) | Vitrification carrier freezing system | |
| CN205661780U (en) | Applicable medical sample drawer type freezes deposits device | |
| CN207054627U (en) | The conserving case of embryo in vitro or cell freezing straw | |
| CN104642299A (en) | Vitrification freezing carrier system | |
| CN206821830U (en) | A kind of rotary-type multi-functional liquid nitrogen container | |
| CN207439788U (en) | A kind of pathology of convenient combined type small sample tissue exempted to wrap up and dismantled fixes embedded box | |
| CN203424215U (en) | Miniature carrier system for organism cryopreservation | |
| CN108617641A (en) | A kind of biological sample glass frozen cryopreservation device | |
| CN203952246U (en) | One is organized refrigerating plant | |
| CN204916573U (en) | Freezing memotron of sample and freezing storage device | |
| CN201104884Y (en) | Field vaccines exemplar sample-taking box | |
| CN206307489U (en) | A kind of laboratory prevents adhesion portable sample freeze box | |
| CN207317336U (en) | A kind of mixed material matter column type freezing storing box | |
| CN208624459U (en) | A kind of vitreous for the carrier cutting-edge structure that recessed spoon and thin slice are combined freezes bar | |
| CN204045124U (en) | Labware Classification Management card | |
| CN214629461U (en) | Freezing support | |
| CN207948733U (en) | Biological sample storage device in a kind of liquid nitrogen container | |
| CN206238189U (en) | Freezing carrier, freezing carrier device and freezing Load System | |
| CN205902715U (en) | Freezing carrier of biological sample that can fix a position freezes deposits support combination |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |