CN104986447B - Sample stored frozen pipe and sample stored frozen device - Google Patents
Sample stored frozen pipe and sample stored frozen device Download PDFInfo
- Publication number
- CN104986447B CN104986447B CN201510315624.7A CN201510315624A CN104986447B CN 104986447 B CN104986447 B CN 104986447B CN 201510315624 A CN201510315624 A CN 201510315624A CN 104986447 B CN104986447 B CN 104986447B
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- China
- Prior art keywords
- sample
- pipe
- auxiliary section
- pipe cap
- action bars
- Prior art date
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
- A01N1/0268—Carriers for immersion in cryogenic fluid, both for slow-freezing and vitrification, e.g. open or closed "straws" for embryos, oocytes or semen
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
- F25D3/00—Devices using other cold materials; Devices using cold-storage bodies
- F25D3/10—Devices using other cold materials; Devices using cold-storage bodies using liquefied gases, e.g. liquid air
- F25D3/107—Devices using other cold materials; Devices using cold-storage bodies using liquefied gases, e.g. liquid air portable, i.e. adapted to be carried personally
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5082—Test tubes per se
- B01L3/50825—Closing or opening means, corks, bungs
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/42—Low-temperature sample treatment, e.g. cryofixation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
- B01L2300/042—Caps; Plugs
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/046—Function or devices integrated in the closure
- B01L2300/047—Additional chamber, reservoir
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/046—Function or devices integrated in the closure
- B01L2300/048—Function or devices integrated in the closure enabling gas exchange, e.g. vents
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0848—Specific forms of parts of containers
- B01L2300/0858—Side walls
Abstract
This application discloses a kind of sample freezer storage pipe, including body and the integral type pipe cap being removably attached in the body；The upper end of the body has upper end open, and its lower end has lower ending opening, is located in the body below the upper end open and is additionally provided with body sleeve pipe, exacerbation portion is additionally provided with the body；The pipe cap includes pipe cap auxiliary section and carries bar with the integrally formed sample in the pipe cap auxiliary section, and the sample carries at least side on bar and is provided with the storage tank for storing sample；Wherein, the pipe cap auxiliary section is removably attached in the upper end open of the body, and the sample carries bar and is inserted into extracting in the body sleeve pipe or out of described body sleeve pipe.Disclosed herein as well is a kind of sample freezer storage pipe device.Sample stored frozen pipe and sample stored frozen device that the application is provided, its is simple in construction, easy to operate, no liquid nitrogen residual.
Description
Technical field
The application is related to sample storing technology field, more particularly to a kind of all kinds of for storing biological, medicine laboratory
The sample stored frozen pipe and sample stored frozen device of tissue or cell.
Background technology
Current IVF use for laboratory is main in the carrier of various organization, cell, egg mother cell and body early embryo freezer storage
Forgive freezing ring, freezing straw and freezing and carry bar three major types.
It is higher that freezing ring has a sample dress loop technique requirement in use, need to be by strict training and skilled operators
Operation can be smoothly completed.And because freezing ring is plastic material, load bar is metal material, between being formed with therebetween necessarily
Gap, causes that liquid nitrogen can be remained in the gap.In melting process, liquid nitrogen volatilization occurs a large amount of bubbles or burst, and causes
The loss of sample.
And freezing straw and freezing carries bar and exists in use cumbersome, body is longer to take substantial amounts of storage
Space, low space utilization, pipe diameter is smaller, sample message can not complete display be marked on body, it is when using easy
Appearance is obscured or uncertain defect.
The content of the invention
The application's aims to overcome that noresidue liquid nitrogen when defect of the prior art is used there is provided one kind, structure letter
Single, easy to operate sample stored frozen pipe and sample stored frozen device.
Technical scheme provides a kind of sample freezer storage pipe, including body and is removably attached to the body
In integral type pipe cap；The upper end of the body has upper end open, and its lower end has lower ending opening, is located in the body
Body sleeve pipe is additionally provided with below the upper end open, exacerbation portion is additionally provided with the body；The pipe cap includes pipe
Cap auxiliary section and carry bar with the integrally formed sample in the pipe cap auxiliary section, the sample, which carries on bar at least side and is provided with, to be used for
Store the storage tank of sample；Wherein, the pipe cap auxiliary section is removably attached in the upper end open of the body, institute
State sample and carry that bar is accommodating to be inserted into extracting in the body sleeve pipe or out of described body sleeve pipe.
Brief description of the drawings
With reference to drawings described below, the feature and its advantage of the present invention is better understood with：
The sectional view for the sample stored frozen pipe that Fig. 1 provides for an embodiment；
Fig. 2 is the side view of body；
Fig. 3 is the schematic diagram that marked area is provided with the body shown in Fig. 2；
Fig. 4 is the side view of pipe cap；
Fig. 5 is the top view of the pipe cap shown in Fig. 4；
The sectional view for the sample stored frozen device that Fig. 6 provides for an embodiment；
Fig. 7 is the side view of control stick；
Fig. 8 is the top view of control stick.
Embodiment
Further illustrate the specific embodiment of the application below in conjunction with the accompanying drawings.Wherein identical parts are attached with identical
Icon note is represented.It should be noted that the word "front", "rear", "left", "right", the "up" and "down" that are used below in description refer to
Be direction in accompanying drawing, word " interior " and " outer " are referred respectively to towards or away from the direction of particular elements geometric center.
The sample stored frozen pipe and sample stored frozen device that the application is provided are mainly used in biological, medicine laboratory
In to various organization, cell, egg mother cell and body early embryo carry out stored frozen be used.By storage to be frozen in the application
Tissue, cell, egg mother cell or body early embryo are referred to as sample, and the pipe and device of freezer storage sample are respectively correspondingly referred to as
For sample freezer storage pipe and sample stored frozen device.
As Figure 1-4, the sample freezer storage pipe 100 that the embodiment of the application one is provided, including body 1 and removably
It is assemblied in the integral type pipe cap 2 in body 1.
The upper end of body 1 has upper end open 11, and its lower end has lower ending opening 12, and upper end open is located in body 1
11 lower section is additionally provided with body sleeve pipe 13, and exacerbation portion 3 is additionally provided with body 1.
Pipe cap 2 includes pipe cap auxiliary section 21 and carries bar 22 with the integrally formed sample in pipe cap auxiliary section 21, and sample carries bar 22
On at least side be provided with the storage tank 221 for storing sample.
Wherein, pipe cap auxiliary section 21 is removably attached in the upper end open 11 of body 1, and sample carries bar 22 and is inserted into
Extracted in body sleeve pipe 13 or out of body sleeve pipe 13.
It that is to say, the sample stored frozen pipe 100 is mainly made up of body 1 and pipe cap 2.Pipe cap 2 is integral type pipe cap, its
Whole part is integrally formed to be formed, therefore the gapless between each connection member of pipe cap 2, will not remain liquid nitrogen.
The upper end of body 1 has upper end open 11, and its lower end has lower ending opening 12.Body is additionally provided with body 1
Sleeve pipe 13, it is located at the lower section of upper end open 11.When sample freezer storage pipe 100 is inserted in liquid nitrogen, liquid nitrogen can be from lower end
Opening 12 enters in body 1, carries bar 22 for the sample to body sleeve pipe 13 and in body sleeve pipe 13 and sample drops
Temperature or insulation.And exacerbation portion 3 is set on body 1, the buoyancy for offsetting the sample freezer storage pipe 100 being in liquid nitrogen.
Exacerbation portion 3 can have weight object to be any, it is possible to be arranged on optional position on body 1.
Pipe cap 2 includes pipe cap auxiliary section 21 and sample carries bar 22, and sample carries bar 22 and is integrally formed with pipe cap auxiliary section 21, shape
Into above-mentioned integral type pipe cap 2, gapless between bar 22 and pipe cap auxiliary section 21 is carried in sample, when pipe cap 2 takes out from liquid nitrogen,
It will not be carried in sample between bar 22 and pipe cap auxiliary section 21 and remain liquid nitrogen, remained when using without liquid nitrogen, it is to avoid during stored frozen
There are a large amount of bubbles or burst in liquid nitrogen volatilization, and causes the defect of the loss of sample.
Carried in sample and storage tank 221 is provided with bar 22, storage tank 221 is located at least one side that sample carries bar 22
On, for storing one or more samples.Sample carries bar 22 and potentially includes two or set two or more in other embodiments
Storage tank 221, two or set the shape or positional symmetry of two or more storage tank 221 to set.In use, sample is placed
In storage tank 221, in moving process, sample will not be lost from storage tank 221.
During connection, pipe cap auxiliary section 21 is assemblied in upper end open 11, and detachably connected with upper end open 11.I.e.
It is that pipe cap auxiliary section 21 may be mounted in upper end open 11, can also be removed from upper end open 11.Upper end open 11 with
Connection between pipe cap auxiliary section 21 can be any type of connection, for example, threaded connection, key connection, pin connection etc. and
Other connected modes.
After connection, sample is carried in the insertion body of bar 22 sleeve pipe 13, and the bottom of body sleeve pipe 13 is closed, storage tank 221
It also is located in body sleeve pipe 13, it is to avoid sample is contacted with the external world and polluted.When pulling down, sample carries bar 22 with pipe cap auxiliary section
21 movement, can be extracted out of body sleeve pipe 13.
Preferably, body 1 is plastic material, its cylindrical or rectangular-shape.Pipe cap 2 is metal material or plastics material
Matter, each part (for example, pipe cap auxiliary section 21 and sample carry bar 22) of pipe cap 2 uses same material, it is ensured that each part it
Between can be integrally formed.
Metal material can use one kind of the various metals materials such as copper, iron, stainless steel, almag, aluminium, tin；Plastics
Material can use polyethylene (PE), polypropylene (PP), polyvinyl chloride (PVC), polystyrene (PS), ABS, polymethylacrylic acid
One kind of a variety of plastic materials such as methyl esters (PMMA), polyamide (PA).
To sum up, the application provide sample stored frozen pipe, by the way that pipe cap is set into integral type, its pipe cap auxiliary section with
Sample carries bar and is integrally formed, and gapless, when pipe cap is taken out from liquid nitrogen, liquid nitrogen is not had and remains in gap between the two
In, it is to avoid there are a large amount of bubbles or burst in liquid nitrogen volatilization during storage, causes the defect of the loss of sample.It is also by sample
Carry and holding tank is set on bar, it is to avoid when mobile, cause sample to lose.
As Figure 1-3, it is preferable that it is in funnelform pars infundibularis 131 that the upper end of body sleeve pipe 13, which has,.Pars infundibularis 131
It is wide at the top and narrow at the bottom, when sample is carried in the insertion body of bar 22 sleeve pipe 13 there is provided guide effect, it is easy to sample load bar 22 inserting body
In sleeve pipe 13.
As shown in Figure 5 and Figure 6, it is preferable that be provided with the upper surface of pipe cap auxiliary section 21 for the operation with outside
The first magnetic portion 25 that bar 4 is connected.By setting first magnetic portion 25, in connection, action bars 4 to be adsorbed
On pipe cap auxiliary section 21 so that action bars 4 links together with pipe cap 2, it is easy to action bars 4 to move pipe cap 2, pipe cap 2 is inserted
Taken out inside liquid nitrogen or from liquid nitrogen.
As shown in Figure 5 and Figure 6, it is preferable that be additionally provided with the upper surface of pipe cap auxiliary section 21 for fixed with action bars 4
The locating aperture portion 26 that position coordinates.During connection, the positioning cams 44 on action bars 4 are inserted in locating aperture portion 26, so that
Action bars 4 is obtained to be firmly coupled to each other with pipe cap 2.
As Figure 1-3, it is preferable that exacerbation portion 3 is metal ball, and metal ball is arranged on body sleeve pipe 13, for offsetting
The buoyancy for the sample freezer storage pipe 100 being in liquid nitrogen.
As shown in Fig. 1-4 and Fig. 6, it is preferable that pipe cap auxiliary section 21 includes being used for what is be connected with outside action bars 4
Top auxiliary section 23 and the bottom auxiliary section 24 for being connected with body 1.
Body connecting portion 14 is provided between upper end open 11 and body sleeve pipe 13, bottom auxiliary section 24 removably connects
It is connected in body connecting portion 14, top auxiliary section 23 is covered in upper end open 11.
Connection between bottom auxiliary section 24 and body connecting portion 14 can be any type of connection, for example, screw thread
Connection, key connection or pin connection etc. and the connection of other connected modes, so that bottom auxiliary section 24 may be mounted at body company
In socket part 14, it can also be pulled down from body connecting portion 14.
In one embodiment, body 1, the cross section of pipe cap 2 are circle.A diameter of 4-22mm of body 1, be highly
9-115mm.A diameter of 4-22mm of the top auxiliary section 23 of pipe cap 2, is highly 1-11mm；A diameter of 2- of bottom auxiliary section 24
19mm, is highly 1-11mm；Sample carries a diameter of 0.4-17mm of bar 22, is highly 4-95mm.In one embodiment, it is above-mentioned
Size can be selected reasonably, to utilize freezer storage space to greatest extent.
As shown in Figure 5, it is preferable that the passage of the upper and lower surface of insertion pipe cap auxiliary section 21 is provided with pipe cap auxiliary section 21
27, passage 27 deviates sample load bar 22 and set.Passage 27 is specifically located on bottom auxiliary section 24, and insertion bottom coordinates
The upper and lower surface in portion 24.Passage 27 carries bar 22 with sample and is not arranged on the same straight line, and it deviates sample load bar 22 and set.Can be with
On the body that passage 27 is arranged on to bottom auxiliary section 24, the edge of bottom auxiliary section 24 can also be disposed at.It is logical
Stomata 27 keeps the air pressure and external balance in body sleeve pipe 13, it is to avoid when liquid nitrogen volatilizees, the internal pressure of body sleeve pipe 13 is excessive,
The top of pipe cap 2 is opened or causes body to burst, sample is lost.
The length of storage tank 221 is 0.5-82mm, and depth is 0.2-8mm, also storage tank 221 can be set into other shapes
Formula.In one embodiment, the section of storage tank 221 is V-shaped or U-shaped.In use, sample is placed on the storage tank of V-arrangement or U-shaped
In 221, when mobile example carries bar 22, sample is kept in the storage tank 221 in V-arrangement or U-shaped, will not be lost.
As Figure 1-3, it is preferable that marked area 15 is provided with body 1.In operation, first it can be recorded in marked area 15
The information of good sample to be dealt with, does not result in and obscures.Tag slot 15 can be rectangle or square；It can be transparent or
Each color coating is scribbled, white coating is preferably coated with, its area is 20mm2-6000mm2。
To sum up, the sample stored frozen pipe that the application is provided, its is simple in construction, easy to operate, no liquid nitrogen residual；It is managed
Body, the height of pipe cap, reasonable wide, freezer storage space can be utilized to greatest extent.And with enough space surfaces, can be
Marked area marks sample message in detail, does not result in and obscures or do not know.
As shown in fig. 6, the sample freezer storage device 200 that the embodiment of the application one is provided, including sample described above
Product stored frozen pipe 100 and action bars 4.
Structure, construction and the operation principle of the sample stored frozen pipe 100 are described in detail in previous section, herein no longer
Repeat.
Action bars 4 is detachably connected on pipe cap auxiliary section 21, so action bars 4 can be connected to pipe cap auxiliary section 21
On, to move pipe cap 2.When the one or more samples of storage, action bars 4 is removed from pipe cap 2, pipe cap 2 is placed on pipe
In body 1, to carry out stored frozen sample.
Preferably, action bars 4 can be metal material or plastic material.Metal material can using copper, iron, stainless steel,
One kind of the various metals material such as almag, aluminium, tin；Plastic material can use polyethylene (PE), polypropylene (PP), polychlorostyrene
A variety of plastic materials such as second dilute (PVC), polystyrene (PS), ABS, polymethyl methacrylate (PMMA), polyamide (PA)
It is a kind of.
To sum up, the sample stored frozen device that the application is provided, by the way that pipe cap is set into integral type, its pipe cap auxiliary section
Bar is carried with sample to be integrally formed, gapless, when pipe cap is taken out from liquid nitrogen, liquid nitrogen is not had and remains in gap between the two
In, it is to avoid there are a large amount of bubbles or burst in liquid nitrogen volatilization during storage, causes the defect of the loss of sample.It is also by sample
Carry and holding tank is set on bar, it is to avoid when mobile, cause sample to lose.Pipe cap is moved by action bars, is easy to operation, with to sample
Product carry out stored frozen.
As Figure 1-3, it is preferable that it is in funnelform pars infundibularis 131 that the upper end of body sleeve pipe 13, which has,.Pars infundibularis 131
It is wide at the top and narrow at the bottom, when sample is carried in the insertion body of bar 22 sleeve pipe 13 there is provided guide effect, it is easy to sample load bar 22 inserting body
In sleeve pipe 13.
As viewed in figures 5-8, it is preferable that be provided with and connect for coordinating with action bars 4 on the upper surface of pipe cap auxiliary section 21
The first magnetic portion 25 connect.
Action bars 4 includes action bars main part 41 and is arranged on the action bars connecting portion 42 of the one end of operating main body portion 41,
The second magnetic portion 43 for coordinating with the first magnetic portion 25 is provided with action bars connecting portion 42.
First magnetic portion 25 and the second magnetic portion 43 can be magnet, in connection, the first magnetic portion 25 and the second magnetic
Portion 43 is attached together, so that action bars 4 is adsorbed on pipe cap auxiliary section 21 so that action bars 4 is connected to one with pipe cap 2
Rise, be easy to action bars 4 to move pipe cap 2, pipe cap 2 is inserted inside liquid nitrogen or taken out from liquid nitrogen.
As viewed in figures 5-8, it is preferable that be additionally provided with the upper surface of pipe cap auxiliary section 21 for being positioned with action bars 4
The locating aperture portion 26 of cooperation.
Correspondingly, the positioning cams 44 for coordinating with locating aperture portion 26 are provided with action bars connecting portion 42.
Locating aperture portion 26 is the breach of indent, and its position is corresponding with positioning cams 43, can be square, circular etc. many
Type.Locating aperture portion 26 can be circle, and its notch diameter is 0.4-6mm, and depth is 0.4-6mm.
Positioning cams 43, are plastics or metal material, and it matches with locating notch portion 26, it is therefore preferable to cylinder,
Its a diameter of 0.4-6mm, depth is 0.4-6mm.
During connection, positioning cams 44 are inserted in locating aperture portion 26, to cause action bars 4 firmly to connect with pipe cap 2
It is connected together.
As Figure 1-3, it is preferable that exacerbation portion 3 is metal ball, and metal ball is arranged on body sleeve pipe 13, for offsetting
The buoyancy for the sample freezer storage pipe 100 being in liquid nitrogen.
As shown in Fig. 1-4 and Fig. 6, it is preferable that pipe cap auxiliary section 21 includes being used for what is be connected with outside action bars 4
Top auxiliary section 23 and the bottom auxiliary section 24 for being connected with body 1.
Body connecting portion 14 is provided between upper end open 11 and body sleeve pipe 13, bottom auxiliary section 24 removably connects
It is connected in body connecting portion 14, top auxiliary section 23 is covered in upper end open 11.
Bottom auxiliary section 24 can be connected through a screw thread with body connecting portion 14, key is connected or the connected mode such as pin connection connects
Connect, so that bottom auxiliary section 24 may be mounted in body connecting portion 14, can also be pulled down from body connecting portion 14.
As shown in Figure 5, it is preferable that the passage of the upper and lower surface of insertion pipe cap auxiliary section 21 is provided with pipe cap auxiliary section 21
27, passage 27 deviates sample load bar 22 and set.Passage 27 is specifically located on bottom auxiliary section 24, and insertion bottom coordinates
The upper and lower surface in portion 24.Passage 27 carries bar 22 with sample and is not arranged on the same straight line, and it deviates sample load bar 22 and set.Can be with
On the body that passage 27 is arranged on to bottom auxiliary section 24, the edge of bottom auxiliary section 24 can also be disposed at.It is logical
Stomata 27 keeps the air pressure and external balance in body sleeve pipe 13, it is to avoid when liquid nitrogen volatilizees, the internal pressure of body sleeve pipe 13 is excessive,
The top of pipe cap 2 is opened or causes body to burst, sample is lost.
The length of storage tank 221 is 0.5-82mm, and depth is 0.2-8mm, also storage tank 221 can be set into other shapes
Formula.The section of storage tank 221 is V-shaped or U-shaped.In use, one or more samples are placed on the storage tank 221 of V-arrangement or U-shaped
Interior, when mobile example carries bar 22, sample is kept in the storage tank 221 in V-arrangement or U-shaped, will not be lost.
As Figure 1-3, it is preferable that marked area 15 is provided with body 1.In operation, first it can be recorded in marked area 15
The information of good sample to be dealt with, does not result in and obscures.Tag slot 15 can be rectangle or square；It can be transparent or
Each color coating is scribbled, white coating is preferably coated with, its area is 20mm2-6000mm2。
The application method for the sample stored frozen device 200 that the application is provided is as follows：
1st, first sample message is filled in or is pasted onto the tag slot 15 of body 1.
2nd, by action bars 4 and pipe cap 2 by the first magnetic portion 25 and the second magnetic portion 43, and locating aperture portion 26 and fixed
Position lug boss 44 links together, and is removed pipe cap 2 from body 1 by action bars 4, being positioned over side together with body 1 treats
With.
3rd, sample is handled by cryoprotector.
4th, one or more samples and a small amount of cryoprotector are positioned in storage tank 221 using transfer tool；Wherein,
One hand of operator grips transfer tool, and a hand grips action bars 4 in addition.Operator can rotate sample and carry depositing on bar 22
The direction of groove 221 is put, it is faced operator.
5th, pipe cap 2 is integrally placed in clean liquid nitrogen rapidly by hand-held bar 4, is placed body 1 under liquid nitrogen liquid level, is protected
Hold pipe cap and body to be placed in together below liquid nitrogen liquid level, stand for a moment.
6th, pipe cap 2 and body 1 are connected under liquid nitrogen liquid level.
7th, action bars 4 is taken off from pipe cap 2, freezer storage pipe 100 is positioned over tweezers or pliers and deposited accordingly
Storage space is put.
8th, do exercises and perform record.
As needed, above-mentioned each technical scheme can be combined, to reach best-of-breed technology effect.
Above-described is only the principle and preferred embodiment of the application.It should be pointed out that for the common skill of this area
For art personnel, on the basis of the application principle, some other modifications can also be made, the protection model of the application is also should be regarded as
Enclose.
Claims (18)
1. a kind of sample freezer storage pipe, it is characterised in that including body and the one being removably attached in the body
Formula pipe cap；
Wherein, the body includes positioned at the upper end open of upper end, the lower ending opening positioned at lower end, in the body and is located at
The body sleeve pipe of the lower section of the upper end open and exacerbation portion；
Wherein, the pipe cap includes pipe cap auxiliary section and carries bar, the sample with the integrally formed sample in the pipe cap auxiliary section
Carry at least side on bar and be provided with the storage tank for storing sample；
Wherein, the pipe cap auxiliary section is removably attached in the upper end open of the body, and the sample carries bar energy
Enough insert in the body sleeve pipe or extracted out of described body sleeve pipe.
2. sample freezer storage pipe according to claim 1, it is characterised in that it is in funnel-form to have on the body sleeve pipe
Pars infundibularis.
3. sample freezer storage pipe according to claim 1, it is characterised in that on the upper surface of the pipe cap auxiliary section
It is provided with the first magnetic portion for being connected with outside action bars.
4. sample freezer storage pipe according to claim 3, it is characterised in that on the upper surface of the pipe cap auxiliary section
It is additionally provided with for the locating aperture portion with the action bars location fit.
5. sample freezer storage pipe according to claim 1, it is characterised in that the exacerbation portion is metal ball, the gold
Belong to ball to be arranged on the body sleeve pipe.
6. sample freezer storage pipe according to claim 1, it is characterised in that the pipe cap auxiliary section include being used for it is outer
The top auxiliary section that the action bars in portion is connected and the bottom auxiliary section for being connected with the body；
Wherein, body connecting portion is provided between the upper end open and the body sleeve pipe, the bottom auxiliary section is removable
It is connected to unloading in the body connecting portion, the top auxiliary section is covered in the upper end open.
7. sample freezer storage pipe according to claim 1, it is characterised in that be provided with insertion on the pipe cap auxiliary section
The passage of pipe cap auxiliary section upper and lower surface, the passage deviates the sample and carries bar setting.
8. sample freezer storage pipe according to claim 1, it is characterised in that the section of the storage tank is V-shaped or U
Shape.
9. sample freezer storage pipe according to claim 1, it is characterised in that be provided with marked area on the body.
10. a kind of sample freezer storage device, it is characterised in that including sample stored frozen pipe and action bars；
Wherein, the sample stored frozen pipe includes body and the integral type pipe cap being removably attached in the body；
Wherein, the body includes positioned at the upper end open of upper end, the lower ending opening positioned at lower end, in the body and is located at
The body sleeve pipe of the lower section of the upper end open and exacerbation portion；
Wherein, the pipe cap includes pipe cap auxiliary section and carries bar, the sample with the integrally formed sample in the pipe cap auxiliary section
Carry at least side on bar and be provided with the storage tank for storing sample；
Wherein, the pipe cap auxiliary section is removably attached in the upper end open of the body, and the sample carries bar energy
Enough insert in the body sleeve pipe or extracted out of described body sleeve pipe；
Wherein, the action bars is removably connected with the pipe cap auxiliary section.
11. sample freezer storage device according to claim 10, it is characterised in that have on the body sleeve pipe in leakage
Bucket-shaped pars infundibularis.
12. sample freezer storage device according to claim 10, it is characterised in that the upper table in the pipe cap auxiliary section
The first magnetic portion for being connected with the action bars is provided with face；
Wherein, the action bars includes action bars main part and is arranged on the action bars connecting portion of operating main body portion one end,
The second magnetic portion for coordinating with first magnetic portion is provided with the action bars connecting portion.
13. sample freezer storage device according to claim 12, it is characterised in that the upper table in the pipe cap auxiliary section
It is additionally provided with face for the locating aperture portion with the action bars location fit；
Wherein, the positioning cams for coordinating with the locating aperture portion are provided with the action bars connecting portion.
14. sample freezer storage device according to claim 10, it is characterised in that the exacerbation portion is metal ball, institute
Metal ball is stated on the body sleeve pipe.
15. sample freezer storage device according to claim 10, it is characterised in that the pipe cap auxiliary section includes being used for
The top auxiliary section being connected with the action bars and the bottom auxiliary section for being connected with the body；
Wherein, body connecting portion is provided between the upper end open and the body sleeve pipe, the bottom auxiliary section is removable
It is connected to unloading in the body connecting portion, the top auxiliary section is covered in the upper end open.
16. sample freezer storage device according to claim 10, it is characterised in that be provided with the pipe cap auxiliary section
The passage of pipe cap auxiliary section upper and lower surface described in insertion, the passage deviates the sample and carries bar setting.
17. sample freezer storage device (200) according to claim 10, it is characterised in that the section of the storage tank
V-shaped or U-shaped.
18. sample freezer storage device according to claim 10, it is characterised in that sign is provided with the body
Area.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510315624.7A CN104986447B (en) | 2015-06-10 | 2015-06-10 | Sample stored frozen pipe and sample stored frozen device |
| US14/882,070 US20160363363A1 (en) | 2015-06-10 | 2015-10-13 | Sample cryogenic storage pipe and device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510315624.7A CN104986447B (en) | 2015-06-10 | 2015-06-10 | Sample stored frozen pipe and sample stored frozen device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104986447A CN104986447A (en) | 2015-10-21 |
| CN104986447B true CN104986447B (en) | 2017-08-11 |
Family
ID=54298380
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510315624.7A Active CN104986447B (en) | 2015-06-10 | 2015-06-10 | Sample stored frozen pipe and sample stored frozen device |
Country Status (2)
| Country | Link |
|---|---|
| US (1) | US20160363363A1 (en) |
| CN (1) | CN104986447B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107372470B (en) * | 2017-09-11 | 2021-05-28 | 马博 | Ovum embryo vitrification cryopreservation device |
| US11116206B2 (en) | 2018-10-01 | 2021-09-14 | Cook Medical Technologies Llc | Cryocontainer |
| CN109362705A (en) * | 2018-10-17 | 2019-02-22 | 广州品知医疗器械有限公司 | A kind of closed glass freezing carrier |
| CN110257229B (en) * | 2019-07-08 | 2023-11-07 | 河南省农业科学院畜牧兽医研究所 | Simple embryo suction device and manufacturing method thereof |
| CN110420674A (en) * | 2019-08-30 | 2019-11-08 | 贵州大学 | A kind of liquid nitrogen takes transfer device |
| CN110476952B (en) * | 2019-09-06 | 2021-05-25 | 苏州贝康医疗器械有限公司 | Vitrification freezing carrier |
| CN111081514A (en) * | 2020-01-06 | 2020-04-28 | 南通大学 | A fixed pipe of transmission electron microscope biological sample for long-range sending |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2731663A (en) * | 1953-10-15 | 1956-01-24 | Thompson Murray | Pot lid with magnetic quick-detachable handle |
| US5296998A (en) * | 1991-11-27 | 1994-03-22 | General Electric Company | Lightning arrester for under-oil applications |
| FR2891166B1 (en) * | 2005-09-28 | 2007-11-23 | Cryo Bio System Sa | PACKAGING ENVELOPE OF A PREDETERMINED VOLUME OF A BIOLOGICAL SUBSTANCE INTENDED TO BE DRAINED IN A LIQUID CRYOGENIC AGENT |
| US8168138B2 (en) * | 2010-12-22 | 2012-05-01 | Li Che | Cryogenic vial |
| US9175549B2 (en) * | 2011-06-06 | 2015-11-03 | Sumathi Paturu | Emergency salvage of a crumbled oceanic oil well |
| EP2916135B1 (en) * | 2012-10-30 | 2018-05-02 | Hitachi High-Technologies Corporation | Reagent container and automated analyzer |
| CN202921332U (en) * | 2012-11-26 | 2013-05-08 | 苏州大学 | Automatic access and identification device of freezing tube |
| US9518898B2 (en) * | 2012-12-06 | 2016-12-13 | Cook Medical Technologies Llc | Cryogenic storage container with sealing closure and methods of using the same |
| CN203152371U (en) * | 2013-04-16 | 2013-08-28 | 曾兴光 | Protective cover in embryo freezing carrier device |
| CN103332395B (en) * | 2013-07-04 | 2016-01-13 | 陈子江 | A kind of cryopreservation tube storage device |
| CN103444699B (en) * | 2013-08-01 | 2015-07-29 | 吕祁峰 | A kind of miniature base system, well-bucket and the cryopreservation methods frozen for biomaterial |
| CN203618631U (en) * | 2013-11-21 | 2014-06-04 | 上海交通大学医学院附属第九人民医院 | Vitrification carrier freezing system |
| US20150204598A1 (en) * | 2014-01-20 | 2015-07-23 | Brooks Automation, Inc. | Portable cryogenic workstation |
| CN204244993U (en) * | 2014-11-19 | 2015-04-08 | 遵义医学院 | A kind of novel embryo's closed glassization freezing year bar and sleeve pipe |
| CN204957348U (en) * | 2015-06-10 | 2016-01-13 | 陈子江 | Freezing memotron of sample and freezing storage device of sample |
-
2015
- 2015-06-10 CN CN201510315624.7A patent/CN104986447B/en active Active
- 2015-10-13 US US14/882,070 patent/US20160363363A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20160363363A1 (en) | 2016-12-15 |
| CN104986447A (en) | 2015-10-21 |
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