US20160363363A1

US20160363363A1 - Sample cryogenic storage pipe and device

Info

Publication number: US20160363363A1
Application number: US14/882,070
Authority: US
Inventors: Zijiang Chen; Keliang Wu; Cheng Li; Jinlong Ma
Original assignee: Shandong Shanda Hospital for Reproductive Medicine Co Ltd
Current assignee: Shandong Shanda Hospital for Reproductive Medicine Co Ltd
Priority date: 2015-06-10
Filing date: 2015-10-13
Publication date: 2016-12-15
Also published as: CN104986447A; CN104986447B

Abstract

The present application discloses a sample cryogenic storage pipe. The sample cryogenic storage pipe includes a pipe body and a one-piece pipe cap removably assembled to the pipe body; the pipe body has an upper opening and a lower opening at the bottom, a pipe sleeve is provided below the upper opening inside the pipe body, and the pipe body is provided with a weighting portion; the pipe cap includes a pipe-cap mating portion and a sample loading rod integrally formed with the pipe-cap mating portion, and the sample loading rod, on at least one side thereof, is provided with a storage groove for storing one or more samples; the pipe-cap mating portion is removably assembled to the upper opening at the top, and the sample loading rod is able to be inserted into the pipe sleeve or be removed from the pipe sleeve. The present application also discloses a sample cryogenic storage device. The sample cryogenic storage pipe and device of the present application have no residual liquid nitrogen trapped therein in use, so that the structure is simple and the operation is easy.

Description

RELATED APPLICATION

This application claims priority to Chinese Patent Application No. 201510315624.7, filed on Jun. 10, 2015, and entitled “SAMPLE CRYOGENIC STORAGE PIPE AND DEVICE,” which is incorporated herein by reference in its entirety.

TECHNICAL FIELD

The present invention relates to sample storage technologies, and more particularly, to a sample cryogenic storage pipe and a sample cryogenic storage device for storing all kinds of tissues and cells of a biological, medical laboratory or the like.

BACKGROUND

Currently, a loading tool, used to cryogenically store all kinds of tissues, cells, oocytes and early embryos in an IVF laboratory, mainly includes three categories: a cryoloop, a cryostraw and a cryogenic loading rod.
There is a higher requirement for a ring mounting technique when the cryoloop is in use. The respective operation can only be smoothly completed by a strictly-trained and skilled operator. And because the cryoloop is made of a plastic material and the loading rod is made of a metal material, a certain gap is formed therebetween, so that liquid nitrogen will reside in the gap. During a melting process, a large amount of bubbles are released or cracks are appeared due to volatilization of the liquid nitrogen, causing loss of one or more samples.
The operation is very complicated when the cryostraw and the cryogenic loading rod are in use. A longer pipe body occupies lots of storage space and space utilization is low. The pipe body has a smaller diameter and sample information cannot be marked clearly and completely on the pipe body, and thus confusion and/or uncertainty easily occurs in use.

SUMMARY

The present application discloses a sample cryogenic storage pipe and a sample cryogenic storage device with features of simple structure and easy operation where there is no residual liquid nitrogen in use.
In one exemplary embodiment, a sample cryogenic storage pipe includes a pipe body and a one-piece pipe cap removably assembled to the pipe body; the pipe body has an upper opening at the top and a lower opening at the bottom, a pipe sleeve is provided below the upper opening inside the pipe body, and the pipe body is provided with a weighting portion; the pipe cap includes a pipe-cap mating portion and a sample loading rod integrally formed with the pipe-cap mating portion, and the sample loading rod, on at least one side thereof, is provided with a storage groove for storing one or more samples; the pipe-cap mating portion is removably assembled to the upper opening, and the sample loading rod is able to be inserted into the pipe sleeve or be removed from the pipe sleeve.

BRIEF DESCRIPTION OF DRAWINGS

These and other features and advantages of the present invention will become better understood with regard to the following description and accompanying drawings in which:

FIG. 1 is a cross-sectional view of an exemplary sample cryogenic storage pipe;

FIG. 2 is a side view of an exemplary pipe body;

FIG. 3 is a side view of the pipe body of FIG. 2, wherein the pipe body includes a marking region;

FIG. 4 is a side view of an exemplary pipe cap;

FIG. 5 is a top view of the pipe cap of FIG. 4;

FIG. 6 is a cross-sectional view of an exemplary sample cryogenic storage device;

FIG. 7 is a side view of an exemplary operating lever; and

FIG. 8 is a top view of an exemplary operating lever.

DETAILED DESCRIPTION

Detailed embodiments of the present application are hereinafter described with reference to the accompanying drawings, wherein identical reference numerals being used to represent identical elements. It should be noted that the words “front”, “back”, “left”, “right”, “up”, “top” and “bottom”, used hereinafter, mean orientations in the drawings, and the words “inside” and “outside” respectively mean the orientations toward and away from geometric center of a certain portion.
The sample cryogenic storage pipe and the sample cryogenic storage device of the present application are mainly used to cryogenically store all kinds of tissues, cells, oocytes and early embryos in a biological or medical laboratory. A tissue or cell to be cryogenically stored is called as a sample, and the pipe and device for cryogenically storing the tissue or cell are correspondingly called as the sample cryogenic storage pipe and the sample cryogenic storage device, respectively.
As shown in FIGS. 1-4, an exemplary sample cryogenic storage pipe 100 includes a pipe body 1 and a one-piece pipe cap 2 removably assembled to the pipe body 1.
The pipe body 1 has an upper opening 11 on the upper end thereof and a lower opening 12 on the lower end thereof. A pipe sleeve 13 is provided below the upper opening 11 within the pipe body 1. The pipe body 1 also includes a weighting portion 3.
The pipe cap 2 includes a pipe-cap mating portion 21 and a sample loading rod 22 integrally formed with the pipe-cap mating portion 21. The sample loading rod 22, on at least one side thereof, is provided with a storage groove 221 for storing one or more samples.
The pipe-cap mating portion 21 removably assembles to the upper opening 11. The sample loading rod 22 is able to be inserted into the pipe sleeve 13 or be removed from the pipe sleeve 13.
That is to say, the sample cryogenic storage pipe 100 mainly consists of the pipe body 1 and the pipe cap 2. The pipe cap 2 is a one-piece pipe cap, which is integrally formed as a whole, so that there is no gap between all of connection portions of the pipe cap 2, preventing residual liquid nitrogen from being trapped in the pipe cap 2.
The pipe body 1 is provided with the upper opening 11 on the upper end thereof and the lower opening 12 on the lower end thereof. The pipe sleeve 13 is also provided within the pipe body 1, which is located below the upper opening 11. When the sample cryogenic storage pipe 100 is inserted into the liquid nitrogen, the liquid nitrogen, may enter the pipe body 1 through the lower opening 12, so as to reduce or maintain the temperature of the sleeve 13, the sample loading rod 22 within the sleeve 13 and the one or more samples therein. Further, the pipe body 1 is provided with the weighting portion 3 for counteracting the buoyant force of the sample cryogenic storage pipe 100 within the liquid nitrogen. The weighting portion 3 may be any object which has weight and can be arranged at any position on the pipe body 1.
The pipe cap 2 includes the pipe-cap mating portion 21 and the sample loading rod 22. The sample loading rod 22 is integrally formed with the pipe-cap mating portion 21, so as to form the above one-piece pipe cap 2. There is no gap between the sample loading rod 22 and the pipe-cap mating portion 21. Therefore, any residual liquid nitrogen is prevented from being trapped when the pipe cap is taken out of the liquid nitrogen. No residual liquid nitrogen is trapped in use, to avoid the defect, i.e. the loss of a sample caused by a large amount of bubbles released by the volatilization of the liquid nitrogen or caused by cracks occurred in the volatilization of the liquid nitrogen during cryogenic storage.
The sample loading rod 22 includes a storage groove 221 on at least one side for storing one or more samples. In some embodiments, the storage groove 221 is located at a lateral side of the sample loading rod 22. In other embodiments, the sample loading rod 22 may include two or more storage grooves 221 that in some cases are symmetrical in shape and/or position. The one or more samples are placed in the storage groove 221 in use. The one or more samples are not lost from the storage groove 221 during the movement of the storage groove 221.
During the connecting operation, the pipe-cap mating portion 21 is configured on the upper opening 11 and removably connected with the upper opening 11, namely, the pipe-cap mating portion 21 can be mounted on the upper opening 11 or removed from the upper opening 11. The connection between the pipe-cap mating portion 21 and the upper opening 11 may be any kind of connection, such as, for example a threaded connection, a keyed connection, a pin connection, or any other suitable connection.
After the connecting operation, the sample loading rod 22 is inserted into the pipe sleeve 13, the bottom of the sleeve 13 is sealed and the storage groove 221 is also located in the pipe sleeve 13, so as to prevent the one or more samples from being polluted by contacting with an environment. When the pipe-cap mating portion 21 is removed, the sample loading rod 22 is moved with the pipe-cap mating portion 21, so as to enable the sample loading rod 22 to be pulled out of the pipe sleeve 13.
Preferably, the pipe body 1, which is of a cylindrical shape or a cuboid shape, is made of a plastic material. The pipe cap 2 is made of a metal material or plastic material, all portions (such as the pipe-cap mating portion 21 and the sample loading rod 22) of which are made of the same material, to ensure that all portions can be integrally formed.
One of various metals (such as copper, iron, stainless steel, aluminum magnesium alloy, aluminum, tin, etc.) can be used as the metal material; and one of various plastics (such as polyethylene (PE), polypropylene (PP), polyvinyl chloride (PVC), polystyrene (PS), ABS, polymethyl methacrylate (PMMA), polyamide (PA), etc.) can be used as the plastic material.
In conclusion, in the sample cryogenic storage pipe of the present application, the pipe cap is arranged in a one-piece manner, the pipe-cap mating portion thereof is integrally formed with the sample loading rod, and there is no gap therebetween, to prevent any residual liquid nitrogen being trapped in the gap when the pipe cap is taken out of the liquid nitrogen, so as to avoid the defect, i.e. the loss of a sample caused by a large amount of bubbles released by the volatilization of the liquid nitrogen or caused by cracks occurred in the volatilization of the liquid nitrogen during storage. During the movement, the loss of one or more samples is avoided.
Now referring to FIGS. 1-3, preferably, the pipe sleeve 13 has a funnel shaped portion 131 that is wide at the top and narrow at the bottom. When the sample loading rod 22 is inserted into the sleeve 13, the funnel shaped portion 131 plays a guiding role, so as to facilitate the sample loading rod 22 being inserted into the pipe sleeve 13.
Now referring to FIGS. 5 and 6, preferably, a first magnetic portion 25 for matching and connecting an external operating lever 4 is provided on an upper surface of the pipe-cap mating portion 21. By providing the first magnetic portion 25, the operating lever 4 is magnetically attracted to the pipe-cap mating portion 21 during the connecting operation, so that the operating lever 4 and the pipe cap 2 are connected together. It is convenient for the operating lever 4 to move the pipe cap 2, so as to insert the pipe cap 2 into the liquid nitrogen or to remove the pipe cap 2 from the liquid nitrogen.
Now referring to FIGS. 5 and 6, preferably, a position opening portion 26 for matching and positioning the operating lever 4 is also provided on the upper surface of the pipe-cap mating portion. The position protrusion 44 on the operating lever is inserted into the position opening portion 26 during the connecting operation, so that the operating lever 4 and the pipe cap 2 are firmly connected together.
Now turning to FIGS. 1-3, preferably, the weighting portion 3 is a metal ball mounted on the pipe sleeve 13 of the pipe body to counteract the buoyant force of the sample cryogenic storage pipe 100 in the liquid nitrogen.
Now referring to FIGS. 1-4 and 6, preferably, the pipe-cap mating portion 21 includes a top mating portion 23 for matching and connecting the external operating lever 4 and a bottom mating portion 24 for matching and connecting the pipe body 1.
A connection portion 14 of the pipe body is provided between the upper opening 11 and the pipe sleeve 13, and the bottom mating portion 24 is removably connected within the connection portion 14 of the pipe body, and the top mating portion 23 covers the upper opening 11.
The connection between the bottom mating portion 24 and the connection portion 14 of the pipe body may be any kind of connection, such as, for example, a threaded connection, a keyed connection, or a pin connection, or any other suitable connection, so that the bottom mating portion 24 can be mounted in the connection portion 14 of the pipe body, and also can be removed from the connection portion 14 of the pipe body.
In some embodiments, the pipe body 1 and the pipe cap 2 each have a circular cross section. The pipe body 1 may have a diameter of 4-22 mm and a height of 9-115 mm. The top mating portion 23 of the pipe cap 2 may have a diameter of 4-22 mm and a height of 1-11 mm; the bottom mating portion 24 may have a diameter of 2-19 mm and a height of 1-11 mm; and the sample loading rod 22 has a diameter of 0.4-17 mm and a height of 4-95 mm. In some embodiments, these dimensions are selected to maximize usage of the cryogenic storage space.
Referring to FIG. 5, preferably, the pipe-cap mating portion 21 is provided with a vent 27 being offset from the sample loading rod 22, which runs through upper and lower surfaces of the pipe-cap mating portion 21. The vent 27 is specifically provided at the bottom mating portion 24 and runs through upper and lower surfaces of the bottom mating portion 24. The vent 27 is offset from the sample loading rod 22 and is not in line with the sample loading rod 22. The vent 27 can be provided on a body of the bottom mating portion 24 or also can be provided at an edge of the bottom mating portion 24. The vent 27 maintains a balance between a pressure in the sleeve 13 of the pipe body and the pressure outside, which avoids the situation that the pipe cap 2 is pushed out or the pipe body bursts due to high pressure in the sleeve 13 of the pipe body when the liquid nitrogen volatilizes, and avoids the lost of the one or more samples.
The storage groove 221 may have a length of 0.5-82 mm and a depth of 0.2-8 mm, and may have any cross-sectional shape. In some embodiments, a cross-section of the storage groove 221 is in a shape of U or in a shape of V. In use, the one or more samples are placed within the V-shaped or U-shaped storage groove 221. When the sample loading rod 22 is moved, the one or more samples remain within the V-shaped or U-shaped storage groove 221 and are not lost.
Now referring to FIGS. 1-3, preferably, the pipe body 1 is provided with a marking region 15. During the operation, information of the sample to be processed can be recorded firstly on the marking region 15 to avoid confusion. The marking region 15 may be rectangular or square, and the marking region 15 may comprise a transparent or colored coating. Preferably, a white coating is adopted. The marking region 15 may cover an area of about 20 mm2 to an area of about 6000 mm2.
In conclusion, the sample cryogenic storage pipe of the present application, has no residual liquid nitrogen trapped therein, and is easy to operate. The height and width of the pipe body and the pipe cap allow full use of the cryogenic storage space, and sufficient surface area so that detailed sample information can be marked in the marking region to avoid confusion and uncertainty.
Now referring to FIG. 6, a sample cryogenic storage device 200 of the present application includes the sample cryogenic storage pipe 100 described above and the operating lever 4.
The structure, construction and working principle of the sample cryogenic storage pipe 100 have been introduced in detail above and will not be described redundantly herein.
The operating lever 4 is removably connectable to the pipe-cap mating portion 21, so that the operating lever 4 can be connected to the pipe-cap mating portion 21 to move the pipe cap 2. When storing the one or more samples, the operating lever 4 is removed from the pipe cap 2, and the pipe cap 2 is placed in the pipe body 1, so as to cryogenically store the one or more samples.
Preferably, the operating lever 4 may be the metal material or the plastic material. One of various metals (such as copper, iron, stainless steel, aluminum magnesium alloy, aluminum, tin, etc.) can be used as the metal material; one of various plastics (such as polyethylene (PE), polypropylene (PP), polyvinyl chloride (PVC), polystyrene (PS), ABS, polymethyl methacrylate (PMMA), polyamide (PA), etc.) can be used as the plastic material.
In conclusion, in an exemplary sample cryogenic storage device of the present application, the pipe cap is arranged in a one-piece manner, the pipe-cap mating portion thereof is integrally formed with the sample loading rod, and there is no gap therebetween, so that no residual liquid nitrogen is trapped in the gap when the pipe cap is taken out of the liquid nitrogen, so as to avoids the defect, i.e. the loss of a sample caused by a large amount of bubbles released by the volatilization of the liquid nitrogen or caused by cracks occurred in the volatilization of the liquid nitrogen during storage. During the movement, the loss of the one or more samples is avoided. The pipe cap is moved by the operating lever, so that it is convenient for operation to cryogenically store the one or more samples.
Now referring to FIGS. 1-3, preferably, the sleeve 13 has a funnel shaped portion 131 that is wide at the top and narrow at the bottom. When the sample loading rod 22 is inserted into the sleeve 13, the funnel shaped portion 131 plays a guiding role, so as to facilitate the sample loading rod 22 being inserted into the sleeve 13.
Now referring to FIGS. 5-8, preferably, the first magnetic portion 25 for matching and connecting an external operating lever 4 is provided on an upper surface of the pipe-cap mating portion 21.
The operating lever 4 includes a main body 41 and a connection portion 42 provided at one end of the main body 41, and a second magnetic portion 43 for matching the first magnetic portion 25 is provided on the connection portion 42 of the operating lever.
Both the first magnetic portion 25 and the second magnetic portion 43 may be magnets. The first magnetic portion 25 and the second magnetic portion 43 stick together during the connecting operation, and thus the operating lever 4 is attracted to the pipe-cap mating portion 21, so that the operating lever 4 and the pipe cap 2 are connected together. Thus, it is convenient for the operating lever 4 to move the pipe cap 2, so as to insert the pipe cap 2 into the liquid nitrogen or to remove the pipe cap 2 from the liquid nitrogen.
Now referring to FIGS. 5-8, preferably, the position opening portion 26 for matching and positioning the operating lever 4 is also provided on the upper surface of the pipe-cap mating portion.
Correspondingly, the connection portion 42 of the operating lever is provided with the position protrusion 44 for matching the position opening portion 26.
The position opening portion 26 is a notch, which may have a plurality of types such as in a square or circular shape, and the position of the notch corresponds to that of the position protrusion. The notch may have a circular shape with a diameter of about 0.4 to about 6 mm and a depth of about 0.4 to about 6 mm.
The position protrusion 44 is made of a metal material or plastic material, coupled with the position notch 26, which is preferably cylindrical with a diameter of about 0.4 to about 6 mm and a depth of about 0.4 to about 6 mm.
The position protrusion 44 is inserted into the position opening portion 26 during the connecting operation, so that the operating lever 4 and the pipe cap 2 are firmly connected together.
Now referring to FIGS. 1-3, preferably, the weighting portion 3 is a metal ball mounted on the sleeve 13 of the pipe body to counteract the buoyant force of the sample cryogenic storage pipe 100 in the liquid nitrogen.
Now referring to FIGS. 1-4 and 6, preferably, the pipe-cap mating portion 21 includes the top mating portion 23 for matching and connecting the external operating lever 4 and the bottom mating portion 24 for matching and connecting the pipe body 1.
The connection portion 14 of the pipe body is provided between the upper opening 11 and the pipe sleeve 13, and the bottom mating portion 24 is removably connected within the connection portion 14 of the pipe body, and the top mating portion 23 covers the upper opening 11.
The bottom mating portion 24 can be connected with the connection portion 14 of the pipe body by a connection way such as the thread connection, the key connection or the pin connection, so that the bottom mating portion 24 can be mounted in the connection portion 14 of the pipe body, and also can be removed from the connection portion 14 of the pipe body.
Now referring to FIG. 5, preferably, the pipe-cap mating portion 21 is provided with the vent 27 that is offset from the sample loading rod 22 that runs through upper and lower surfaces of the pipe-cap mating portion 21. The vent 27 is specifically provided at the bottom mating portion 24, and runs through upper and lower surfaces of the bottom mating portion 24. The vent 27 is offset from the sample loading rod 22 and is not in line with the sample loading rod 22. The vent 27 can be provided on a body of the bottom mating portion 24 or also can be provided at an edge of the bottom mating portion 24. The vent 27 maintains a balance between a pressure in the sleeve 13 of the pipe body and the pressure outside, which prevents the pipe cap 2 from pushed out or the pipe body from bursting due to high pressure in the sleeve 13 of the pipe body when the liquid nitrogen volatilizes, and avoids the loss of the one or more samples.
The storage groove 221 may have a length of 0.5-82 mm and a depth of 0.2-8 mm, and may have any cross-sectional shape. In some embodiments, a cross-section of the storage groove 221 is in a shape of U or in a shape of V. In use, the one or more samples are placed within the V-shaped or U-shaped storage groove 221. When the sample loading rod 22 is moved, the one or more samples remain within the V-shaped or U-shaped storage groove 221 and are not lost.
Now referring to FIGS. 1-3, preferably, the pipe body 1 is provided with a marking region 15. During the operation, information of the sample to be processed can be recorded firstly on the marking region 15 to avoid confusion. The marking region 15 may be rectangular or square, and the marking region 15 may comprise a transparent or colored coating. Preferably, a white coating is adopted. The marking region 15 may cover an area of about 20 mm2 up to area of about 6000 mm2.
The sample cryogenic storage device 200 of the present application may be used in the following way:

- 1. filling or pasting the sample information in the marking region 15 of the pipe body 1;
- 2. connecting the operating lever 4 and the pipe cap 2 together through the first magnetic portion 25 and the second magnetic portion 26, and the position opening portion 26 and the position protrusion 44, and removing the pipe cap 2 from the pipe body 1 through the operating lever 4, and then setting the pipe cap 2 aside along with the pipe body 1, waiting to be used;
- 3. processing the one or more samples with a cryoprotective agent;
- 4. placing the one or more samples and an amount of the cryoprotective agent within the storage groove 221 with a transferring tool; wherein the transferring tool may be held in one hand of an operator and the operating lever 4 may be held in the other hand of the operator; and wherein the operator may orient the storage groove 221 of the sample rod 22 so that it faces towards the operator;
- 5. placing the whole pipe cap within clean liquid nitrogen by the operating lever in the hand, placing the pipe body 1 below the surface of the liquid nitrogen, and holding both the pipe cap and pipe body below the surface of the liquid nitrogen for some time;
- 6. connecting the pipe cap 2 with the pipe body 1 below the surface of the liquid nitrogen;
- 7. removing the operating lever 4 from the pipe cap 2 and placing the sample cryogenic storage pipe 100 in a storage position with a pair of tweezers or forceps; and
- 8. making a record of the operation.

The technical solutions mentioned above can be combined as required to reach best technical effect.
The foregoing is merely a principle and preferred embodiments of the present invention. It should be pointed out that several other variants also can be made on the basis of the principle of the present invention, which shall be included in the protection scope of the present invention.

Claims

What is claimed is:

1. A sample cryogenic storage pipe, comprising:

a pipe body, comprising:

an upper opening,

a lower opening,

a pipe sleeve inside the pipe body and below the upper opening, and

a weighting portion, and

a pipe cap removably coupled to the pipe body, comprising:

a mating portion removably coupled to the upper opening of the pipe body,

a sample loading rod integrally formed with the mating portion, configured to insert into and to remove from the pipe sleeve, and

a storage groove for storing one or more samples, disposed on at least one side of the sample loading rod.

2. The sample cryogenic storage pipe according to claim 1 wherein the pipe sleeve comprises a funnel-shaped portion.

3. The sample cryogenic storage pipe according to claim 1 wherein the mating portion comprises a first magnetic portion for matching and connecting an operating lever, wherein the first magnetic portion is disposed on an upper surface of the mating portion.

4. The sample cryogenic storage pipe according to claim 3 wherein the mating portion further comprises a position opening portion for matching and positioning the operating lever, wherein the position opening portion is disposed on the upper surface of the mating portion.

5. The sample cryogenic storage pipe according to claim 1 wherein the weighting portion is a metal ball mounted on the pipe sleeve.

6. The sample cryogenic storage pipe according to claim 1 wherein the pipe body further comprises a connection portion between the upper opening and the pipe sleeve, wherein the mating portion comprises:

a top mating portion for matching and connecting an operating lever, wherein the top mating portion covers the upper opening, and

a bottom mating portion for matching and connecting the pipe body, wherein the bottom mating portion is removably coupled within the connection portion.

7. The sample cryogenic storage pipe according to claim 1 wherein the mating portion further comprises a vent connecting an upper surface and a lower surface of the mating portion, wherein the vent is spaced apart from the sample loading rod.

8. The sample cryogenic storage pipe according to claim 1 wherein the storage groove has a cross-sectional of a U-shape or a V-shape.

9. The sample cryogenic storage pipe according to claim 1 wherein the pipe body comprises a marking region.

10. A sample cryogenic storage device, comprising:

a sample cryogenic storage pipe, comprising:

a pipe body, comprising:

an upper opening,

a lower opening,

a pipe sleeve inside the pipe body and below the upper opening, and

a weighting portion, and

a pipe cap removably coupled to the pipe body, comprising:

a mating portion removably coupled to the upper opening of the pipe body,

a storage groove for storing one or more samples, disposed on at least one side of the sample loading rod, and

an operating lever configured to removably couple to the mating portion of the pipe cap.

11. The sample cryogenic storage device according to claim 10 wherein the pipe sleeve comprises a funnel-shaped portion.

12. The sample cryogenic storage device according to claim 10 wherein the mating portion comprises a first magnetic portion for matching and connecting an operating lever, wherein the first magnetic portion is disposed on an upper surface of the mating portion, wherein the operating lever comprises:

a main body,

a connecting portion at an end of the main body, and

a second magnetic portion for matching the first magnetic portion, wherein the second magnetic portion is disposed on the connection portion of the operating lever.

13. The sample cryogenic storage device according to claim 12 wherein the mating portion further comprises a position opening portion for matching and positioning the operating lever, wherein the position opening portion is disposed on the upper surface of the mating portion, and wherein the connecting portion of the operating lever comprises a position protrusion for matching the position opening portion.

14. The sample cryogenic storage device according to claim 10 wherein the weighting portion is a metal ball mounted on the pipe sleeve.

15. The sample cryogenic storage device according to claim 10 wherein the pipe body further comprises a connection portion between the upper opening and the pipe sleeve, wherein the mating portion comprises:

16. The sample cryogenic storage device according to claim 10 wherein the mating portion further comprises a vent connecting an upper surface and a lower surface of the mating portion, wherein the vent is spaced apart from the sample loading rod.

17. The sample cryogenic storage device according to claim 10, wherein the storage groove has a cross-sectional of a U-shape or a V-shape.

18. The sample cryogenic storage device according to claim 10, wherein the pipe body comprises a marking region.